Rh4BG193100

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003153
Physical Location & Seq
Reverse (-)
33350034 .. 33352224
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG193100.1

Sequence Viewer

Length: 660 bp
ATGGCTGCTGTGAGGTTGAACGCAAAGATTTTCACCGACGGCTACTTCTCCTTGTACCCCTGCAAGCTCGAAGAGGTCAACATCAAGTGCGGCGAGATCTCAAAATTTACAGATCTCAAACCCGGCAGCTGTTCCAGTTTCACGCTCCACCTTCTTCATTTTATACATCGAGCGGTCTCTCTCTTTTCCTCTGCGACTAGTCTTCCCACCCTTTGGAGCTCGCTGTCACCGCCATTGCCTCGAACCGAAGAAGCTCTTGGTACCCTACCCGGTAGAACTCGTTTTCTGAATAAGTGTTCAGTTTCAACAAGTTTTTCTGTACAAGCTTCCTTGCCGGAGAATAGCGATAAATTTAAAGTGGAATACACTCCATGGATGATTGTTGGATTGGGTAACCCTGAAAATAAGTACCATGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAATGCAAGGCATTGTGATGAATACAATTCAGTCAAAGGCACTGGTTGGAATAGTGGGGAAGCGAGTTTATTTTGAGTTTCAGCATAATGTTTGTGGTAGATCAATATATGCAGAGGGTACAGTTGATGCTGTAATTTTCCTTGCTAAGAAGACGCAGTTCATGATGGAAACCATGACAGCTCTCATTGAGAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

219

Amino Acids

24.4

Weight (kDa)

8.94

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 126 - 171 4.7e-12 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 260
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 213
AccBSI CCGCTC 1 cut(s) 173
AciI CCGC 3 cut(s) 90, 173, 230
AcsI RAATTY 2 cut(s) 104, 350
AfaI GTAC 5 cut(s) 56, 262, 321, 410, 580
AfiI CCNNNNNNNGG 1 cut(s) 213
AgsI TTSAA 3 cut(s) 19, 306, 440
AhlI ACTAGT 1 cut(s) 197
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AluBI AGCT 7 cut(s) 67, 129, 219, 254, 326, 641, 655
AluI AGCT 7 cut(s) 67, 129, 219, 254, 326, 641, 655
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 181
ApeKI GCWGC 2 cut(s) 5, 126
ApoI RAATTY 2 cut(s) 104, 350
Asp718I GGTACC 1 cut(s) 260
AsuC2I CCSGG 2 cut(s) 123, 270
AsuHPI GGTGA 2 cut(s) 25, 219
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 194, 617
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 138
BccI CCATC 1 cut(s) 619
BceAI ACGGC 1 cut(s) 55
BcnI CCSGG 2 cut(s) 123, 270
BcoDI GTCTC 1 cut(s) 181
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 2 cut(s) 198, 420
BfmI CTRYAG 1 cut(s) 656
BglII AGATCT 2 cut(s) 96, 112
BisI GCNGC 3 cut(s) 6, 91, 127
BlsI GCNGC 3 cut(s) 7, 92, 128
Bme1390I CCNGG 2 cut(s) 123, 270
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 2 cut(s) 123, 270
BmsI GCATC 1 cut(s) 577
BpiI GAAGAC 2 cut(s) 194, 617
BpuMI CCSGG 2 cut(s) 123, 270
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 2 cut(s) 371, 412
Bsc4I CCNNNNNNNGG 1 cut(s) 213
Bse1I ACTGG 2 cut(s) 135, 507
Bse3DI GCAATG 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 371, 412
BseGI GGATG 1 cut(s) 381
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMI GCAATG 1 cut(s) 233
BseNI ACTGG 2 cut(s) 135, 507
BseXI GCAGC 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 3 cut(s) 123, 270, 335
BslI CCNNNNNNNGG 1 cut(s) 213
BsmAI GTCTC 1 cut(s) 181
Bso31I GGTCTC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 221
Bsp1407I TGTACA 1 cut(s) 319
Bsp143I GATC 3 cut(s) 96, 112, 560
Bsp19I CCATGG 2 cut(s) 371, 412
BspACI CCGC 3 cut(s) 90, 173, 230
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 181
BsrBI CCGCTC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 319
BsrI ACTGG 2 cut(s) 135, 507
BssECI CCNNGG 2 cut(s) 371, 412
BssMI GATC 3 cut(s) 96, 112, 560
BssT1I CCWWGG 2 cut(s) 371, 412
Bst4CI ACNGT 1 cut(s) 583
Bst6I CTCTTC 1 cut(s) 66
BstAUI TGTACA 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 65, 221
BstDEI CTNAG 1 cut(s) 606
BstDSI CCRYGG 2 cut(s) 371, 412
BstEII GGTNACC 1 cut(s) 392
BstF5I GGATG 1 cut(s) 381
BstKTI GATC 3 cut(s) 99, 115, 563
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 3 cut(s) 96, 112, 560
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstPI GGTNACC 1 cut(s) 392
BstSCI CCNGG 2 cut(s) 121, 268
BstSFI CTRYAG 1 cut(s) 656
BstV1I GCAGC 1 cut(s) 138
BstV2I GAAGAC 2 cut(s) 194, 617
BstX2I RGATCY 2 cut(s) 96, 112
BstYI RGATCY 2 cut(s) 96, 112
BtgI CCRYGG 2 cut(s) 371, 412
BtsCI GGATG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 500
Cac8I GCNNGC 2 cut(s) 65, 221
CciI TCATGA 1 cut(s) 621
CseI GACGC 1 cut(s) 622
Csp6I GTAC 5 cut(s) 55, 261, 320, 409, 579
CviAII CATG 4 cut(s) 372, 413, 622, 634
CviJI RGCY 9 cut(s) 5, 42, 67, 129, 219, 254, 326, 641, 655
CviKI_1 RGCY 9 cut(s) 5, 42, 67, 129, 219, 254, 326, 641, 655
CviQI GTAC 5 cut(s) 55, 261, 320, 409, 579
DdeI CTNAG 1 cut(s) 606
DpnI GATC 3 cut(s) 98, 114, 562
DpnII GATC 3 cut(s) 96, 112, 560
DraI TTTAAA 1 cut(s) 355
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Ecl136II GAGCTC 1 cut(s) 219
Eco130I CCWWGG 2 cut(s) 371, 412
Eco24I GRGCYC 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 219
EcoO65I GGTNACC 1 cut(s) 392
EcoT14I CCWWGG 2 cut(s) 371, 412
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 2 cut(s) 371, 412
FaeI CATG 4 cut(s) 375, 416, 625, 637
FaiI YATR 8 cut(s) 164, 373, 414, 546, 568, 570, 623, 635
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 4 cut(s) 371, 412, 621, 633
Fnu4HI GCNGC 3 cut(s) 6, 91, 127
FokI GGATG 1 cut(s) 388
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 3 cut(s) 6, 91, 127
FspBI CTAG 2 cut(s) 198, 420
GluI GCNGC 3 cut(s) 6, 91, 127
HapII CCGG 3 cut(s) 123, 270, 335
HgaI GACGC 1 cut(s) 622
Hin1II CATG 4 cut(s) 375, 416, 625, 637
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 1 cut(s) 324
HpaII CCGG 3 cut(s) 123, 270, 335
HphI GGTGA 2 cut(s) 25, 219
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 288
Hpy188III TCNNGA 1 cut(s) 622
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 1 cut(s) 583
HpyCH4V TGCA 3 cut(s) 63, 466, 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
HpyF3I CTNAG 1 cut(s) 606
Hsp92II CATG 4 cut(s) 375, 416, 625, 637
KpnI GGTACC 1 cut(s) 264
Kzo9I GATC 3 cut(s) 96, 112, 560
LmnI GCTCC 2 cut(s) 150, 216
LpnPI CCDG 7 cut(s) 73, 136, 148, 283, 348, 411, 488
Lsp1109I GCAGC 1 cut(s) 138
LweI GCATC 1 cut(s) 577
MaeI CTAG 2 cut(s) 198, 420
MaeIII GTNAC 2 cut(s) 225, 392
MalI GATC 3 cut(s) 98, 114, 562
MbiI CCGCTC 1 cut(s) 173
MboI GATC 3 cut(s) 96, 112, 560
MboII GAAGA 5 cut(s) 83, 146, 194, 260, 622
MflI RGATCY 2 cut(s) 96, 112
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 4 cut(s) 104, 350, 486, 594
MmeI TCCRAC 2 cut(s) 364, 487
MnlI CCTC 5 cut(s) 6, 67, 199, 249, 568
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 1 cut(s) 476
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 3 cut(s) 123, 270, 335
MspR9I CCNGG 2 cut(s) 123, 270
MwoI GCNNNNNNNGC 1 cut(s) 229
NciI CCSGG 2 cut(s) 123, 270
NcoI CCATGG 2 cut(s) 371, 412
NdeII GATC 3 cut(s) 96, 112, 560
NlaIII CATG 4 cut(s) 375, 416, 625, 637
NlaIV GGNNCC 1 cut(s) 262
NmuCI GTSAC 1 cut(s) 225
PagI TCATGA 1 cut(s) 621
PflMI CCANNNNNTGG 1 cut(s) 213
PkrI GCNGC 3 cut(s) 7, 92, 128
Psp124BI GAGCTC 1 cut(s) 221
PspEI GGTNACC 1 cut(s) 392
PspN4I GGNNCC 1 cut(s) 262
PsuI RGATCY 2 cut(s) 96, 112
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 5 cut(s) 56, 262, 321, 410, 580
RsaNI GTAC 5 cut(s) 55, 261, 320, 409, 579
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 221
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 3 cut(s) 6, 91, 127
Sau3AI GATC 3 cut(s) 96, 112, 560
ScrFI CCNGG 2 cut(s) 123, 270
SduI GDGCHC 1 cut(s) 221
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 476
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 4 cut(s) 104, 350, 486, 594
SsiI CCGC 3 cut(s) 90, 173, 230
SspMI CTAG 2 cut(s) 198, 420
SstI GAGCTC 1 cut(s) 221
StyD4I CCNGG 2 cut(s) 121, 268
StyI CCWWGG 2 cut(s) 371, 412
TaaI ACNGT 1 cut(s) 583
TaqI TCGA 3 cut(s) 69, 169, 241
TasI AATT 4 cut(s) 104, 350, 486, 594
TatI WGTACW 1 cut(s) 319
TauI GCSGC 1 cut(s) 93
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 1 cut(s) 507
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 2 cut(s) 5, 126
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 3 cut(s) 146, 494, 610
TspRI CASTG 1 cut(s) 507
Van91I CCANNNNNTGG 1 cut(s) 213
XapI RAATTY 2 cut(s) 104, 350
XspI CTAG 2 cut(s) 198, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.