Rorug04G0217000

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003153
Physical Location & Seq
Forward (+)
37515645 .. 37516104
460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0217000.1

Sequence Viewer

Length: 423 bp
ATGGCTGAGGAAATGGTGGCACAACTATCGGCGATGTTGGTTAATTCGGCTGCAATTGCGGAAACTTTCTTCTACCTCGTTGGAAGAAGGGTCAGTCACAAGGCCGGGAAATGGGTGCCAAGAACCCTAGCCAAAGTGTGGGGCTTGAAGGATCGCCTCTCGATCACTGTACAAGCTGAGGGACGGTTCTTGTTCCATTTCAAAAAGAAGGAGGAGTGCAACAAAATCCTCTACAGCGGCCCCTGGCATTTCAATGGCCATATGTTTCTGGGTGAATATGATGGTTTGGTTAATGTCGCCGGCGTGTCGCTGTCTTCTTTGAAAATTTGGGTGGTGGTGAGGAAGCTACCACCACAGCTGAGGACAGAGCAGTTGCTAATACTCTTGGGATCCTCAATGGGAAGATTTTTGCACGTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

140

Amino Acids

15.86

Weight (kDa)

9.81

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 41 - 90 2.4e-10 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 115
AccB7I CCANNNNNTGG 1 cut(s) 138
AciI CCGC 2 cut(s) 59, 237
AclWI GGATC 3 cut(s) 159, 384, 397
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 1 cut(s) 324
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 2 cut(s) 111, 138
AgsI TTSAA 4 cut(s) 148, 202, 253, 322
AjnI CCWGG 1 cut(s) 242
AluBI AGCT 3 cut(s) 176, 346, 358
AluI AGCT 3 cut(s) 176, 346, 358
AlwI GGATC 3 cut(s) 159, 384, 397
AoxI GGCC 3 cut(s) 102, 238, 256
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 239
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 284, 349
BalI TGGCCA 1 cut(s) 258
BamHI GGATCC 1 cut(s) 389
BanI GGYRCC 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 306
BbvCI CCTCAGC 3 cut(s) 6, 177, 359
BbvI GCAGC 1 cut(s) 37
BccI CCATC 1 cut(s) 275
BcgI CGANNNNNNTGC 2 cut(s) 9, 43
BciT130I CCWGG 1 cut(s) 244
BcnI CCSGG 1 cut(s) 106
BfaI CTAG 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 232
BisI GCNGC 2 cut(s) 51, 238
BlsI GCNGC 2 cut(s) 52, 239
Bme1390I CCNGG 2 cut(s) 106, 244
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 3 cut(s) 117, 241, 391
BmrFI CCNGG 2 cut(s) 106, 244
BpiI GAAGAC 1 cut(s) 306
Bpu10I CCTNAGC 3 cut(s) 6, 177, 359
BpuMI CCSGG 1 cut(s) 106
BsaJI CCNNGG 1 cut(s) 242
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 138
Bse118I RCCGGY 1 cut(s) 299
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 111, 138
BseMII CTCAG 2 cut(s) 168, 350
BseRI GAGGAG 1 cut(s) 227
BseXI GCAGC 1 cut(s) 37
BshFI GGCC 3 cut(s) 104, 240, 258
BshNI GGYRCC 1 cut(s) 115
BsiSI CCGG 2 cut(s) 105, 300
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 2 cut(s) 111, 138
BsmFI GGGAC 1 cut(s) 195
BsnI GGCC 3 cut(s) 104, 240, 258
Bsp1407I TGTACA 1 cut(s) 169
Bsp143I GATC 3 cut(s) 151, 162, 389
BspACI CCGC 2 cut(s) 59, 237
BspANI GGCC 3 cut(s) 104, 240, 258
BspCNI CTCAG 2 cut(s) 169, 351
BspLI GGNNCC 3 cut(s) 117, 241, 391
BspPI GGATC 3 cut(s) 159, 384, 397
BspT107I GGYRCC 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 299
BsrGI TGTACA 1 cut(s) 169
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 3 cut(s) 151, 162, 389
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 2 cut(s) 169, 186
BstAUI TGTACA 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 3 cut(s) 6, 177, 359
BstKTI GATC 3 cut(s) 154, 165, 392
BstMBI GATC 3 cut(s) 151, 162, 389
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNI CCWGG 1 cut(s) 244
BstSCI CCNGG 2 cut(s) 104, 242
BstSFI CTRYAG 1 cut(s) 232
BstV1I GCAGC 1 cut(s) 37
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
BsuRI GGCC 3 cut(s) 104, 240, 258
BtgZI GCGATG 1 cut(s) 47
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 1 cut(s) 301
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 170
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 3 cut(s) 6, 177, 359
DpnI GATC 3 cut(s) 153, 164, 391
DpnII GATC 3 cut(s) 151, 162, 389
EaeI YGGCCR 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 242
FaiI YATR 3 cut(s) 261, 263, 279
FaqI GGGAC 1 cut(s) 195
FauNDI CATATG 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 51, 238
Fsp4HI GCNGC 2 cut(s) 51, 238
FspBI CTAG 1 cut(s) 128
GluI GCNGC 2 cut(s) 51, 238
HaeIII GGCC 3 cut(s) 104, 240, 258
HapII CCGG 2 cut(s) 105, 300
HpaII CCGG 2 cut(s) 105, 300
HphI GGTGA 2 cut(s) 284, 349
Hpy188III TCNNGA 1 cut(s) 160
HpyAV CCTTC 3 cut(s) 81, 142, 202
HpyCH4III ACNGT 2 cut(s) 169, 186
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 3 cut(s) 53, 219, 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpyF3I CTNAG 3 cut(s) 6, 177, 359
HpySE526I ACGT 1 cut(s) 414
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
Kzo9I GATC 3 cut(s) 151, 162, 389
LpnPI CCDG 5 cut(s) 118, 229, 254, 256, 313
Lsp1109I GCAGC 1 cut(s) 37
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 95
MalI GATC 3 cut(s) 153, 164, 391
MboI GATC 3 cut(s) 151, 162, 389
MboII GAAGA 4 cut(s) 61, 96, 306, 414
MfeI CAATTG 1 cut(s) 54
MflI RGATCY 1 cut(s) 389
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 3 cut(s) 43, 54, 324
MluNI TGGCCA 1 cut(s) 258
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 8 cut(s) 86, 167, 172, 205, 239, 333, 354, 403
Mox20I TGGCCA 1 cut(s) 258
MreI CGCCGGCG 1 cut(s) 299
MroNI GCCGGC 1 cut(s) 299
MscI TGGCCA 1 cut(s) 258
MseI TTAA 3 cut(s) 42, 291, 421
MslI CAYNNNNRTG 1 cut(s) 252
Msp20I TGGCCA 1 cut(s) 258
MspA1I CMGCKG 2 cut(s) 237, 358
MspI CCGG 2 cut(s) 105, 300
MspR9I CCNGG 2 cut(s) 106, 244
MunI CAATTG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 244
MwoI GCNNNNNNNGC 1 cut(s) 56
NaeI GCCGGC 1 cut(s) 301
NciI CCSGG 1 cut(s) 106
NdeI CATATG 1 cut(s) 261
NdeII GATC 3 cut(s) 151, 162, 389
NgoMIV GCCGGC 1 cut(s) 299
NlaIV GGNNCC 3 cut(s) 117, 241, 391
NmuCI GTSAC 1 cut(s) 95
PdiI GCCGGC 1 cut(s) 301
PflMI CCANNNNNTGG 1 cut(s) 138
PkrI GCNGC 2 cut(s) 52, 239
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 3 cut(s) 117, 241, 391
PspPI GGNCC 1 cut(s) 239
PsuI RGATCY 1 cut(s) 389
PvuII CAGCTG 1 cut(s) 358
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 3 cut(s) 42, 291, 421
SatI GCNGC 2 cut(s) 51, 238
Sau3AI GATC 3 cut(s) 151, 162, 389
Sau96I GGNCC 1 cut(s) 239
ScrFI CCNGG 2 cut(s) 106, 244
SetI ASST 5 cut(s) 78, 178, 348, 360, 417
SfcI CTRYAG 1 cut(s) 232
SgrAI CRCCGGYG 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 252
Sse9I AATT 3 cut(s) 43, 54, 324
SsiI CCGC 2 cut(s) 59, 237
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 2 cut(s) 104, 242
TaaI ACNGT 2 cut(s) 169, 186
TaiI ACGT 1 cut(s) 417
TaqI TCGA 1 cut(s) 161
TasI AATT 3 cut(s) 43, 54, 324
TatI WGTACW 1 cut(s) 169
TauI GCSGC 1 cut(s) 240
Tru1I TTAA 3 cut(s) 42, 291, 421
Tru9I TTAA 3 cut(s) 42, 291, 421
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 95
TspRI CASTG 1 cut(s) 172
Van91I CCANNNNNTGG 1 cut(s) 138
XapI RAATTY 1 cut(s) 324
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.