Rh7BG345800

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0003153
Physical Location & Seq
Reverse (-)
36148621 .. 36152236
3616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG345800.1

Sequence Viewer

Length: 798 bp
ATGGCAGCAGTCTCTCTCTTTTCCTCTGCGACTAGTCTCCCCACCCTTTGGAGCTCGCTGTCACCGCCATTGCCTCGAACCGAAGAAGCTCTCGGTACCCTACCCGGTAGAACTCGTTTTCTGAATAAGTGTTCAGTTTCAACAAGTTTTTCTGTACAAGCTTCCTTGCCCGAGAATAATAGCGATAGATTTAAAGTGGAATACACTCCTTGGATGATTGTTGGATTGGGTAACCCTGGAAATAAGTACCATGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAACGCAGGGCATTGTGATGAATACAATTCAGTCAAAGGCGCTGGTTGGAATAGGTTCCATTGGTGAAGTACCGATTTTGGTGGTGAAGCCTCAATCTTACATGAATTATAGTGGGGAAGCGGTTGGACCACTTGCAGCATATTATCAAGTACCTCTACGTCATATATTATTGGTTTATGATGAGATGAGCCTACCAAATGGTGTTCTAAGATTGCAGCCAAAAGGAGGACATGGCTATCACAATGGTGTGAAGAGTGTAGTGGGCCATCTGGATGGCTGCCGTGATTTTCCTCGCTTATGTATAGGTATTGGAAATCCACCTGGTACTATGGACGTGAAAGCCTATCTTCTACAAAAATTCAGTACAGTAGAAAGACATCAGATTGATGCAGCACTGGGTCAAGGCGTTGAGGCTGTGAAGACACTGGTGCTGAATGGATTTGACCCGAATATCACTAGTTTCAATTTGAGGCAAAAATACAAGTATCACAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

265

Amino Acids

28.92

Weight (kDa)

9.28

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 72 - 255 1.3e-56 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 95
AccB7I CCANNNNNTGG 1 cut(s) 48
AciI CCGC 2 cut(s) 65, 419
AcsI RAATTY 1 cut(s) 656
AdeI CACNNNGTG 1 cut(s) 793
AfaI GTAC 7 cut(s) 97, 156, 248, 369, 450, 625, 664
AfiI CCNNNNNNNGG 2 cut(s) 48, 524
AgsI TTSAA 3 cut(s) 141, 278, 763
AhlI ACTAGT 2 cut(s) 32, 755
AjiI CACGTC 1 cut(s) 634
AjnI CCWGG 2 cut(s) 235, 619
AleI CACNNNNGTG 1 cut(s) 543
AluBI AGCT 3 cut(s) 54, 89, 161
AluI AGCT 3 cut(s) 54, 89, 161
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 16, 41
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 1 cut(s) 562
ApeKI GCWGC 5 cut(s) 5, 434, 514, 576, 689
ApoI RAATTY 1 cut(s) 656
Asp700I GAANNNNTTC 1 cut(s) 352
Asp718I GGTACC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 2 cut(s) 425, 562
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 3 cut(s) 54, 374, 394
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 425
BanI GGYRCC 1 cut(s) 95
BanII GRGCYC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 725
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 5 cut(s) 17, 446, 526, 563, 701
BccI CCATC 2 cut(s) 566, 573
BceAI ACGGC 1 cut(s) 564
BciT130I CCWGG 2 cut(s) 237, 621
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 2 cut(s) 16, 41
BcuI ACTAGT 2 cut(s) 32, 755
BfaI CTAG 3 cut(s) 33, 258, 756
BfoI RGCGCY 1 cut(s) 341
BisI GCNGC 5 cut(s) 6, 435, 515, 577, 690
BlsI GCNGC 5 cut(s) 7, 436, 516, 578, 691
Bme1390I CCNGG 3 cut(s) 105, 237, 621
Bme18I GGWCC 1 cut(s) 425
BmeT110I CYCGRG 1 cut(s) 170
BmgBI CACGTC 1 cut(s) 634
BmgT120I GGNCC 2 cut(s) 425, 562
BmiI GGNNCC 2 cut(s) 97, 355
BmrFI CCNGG 3 cut(s) 105, 237, 621
BmrI ACTGGG 1 cut(s) 704
BmsI GCATC 1 cut(s) 676
BmuI ACTGGG 1 cut(s) 704
BpiI GAAGAC 1 cut(s) 725
BpuMI CCSGG 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 209, 235, 250
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 524
Bse1I ACTGG 2 cut(s) 699, 729
Bse3DI GCAATG 1 cut(s) 68
BseBI CCWGG 2 cut(s) 237, 621
BseDI CCNNGG 3 cut(s) 209, 235, 250
BseGI GGATG 2 cut(s) 219, 577
BseLI CCNNNNNNNGG 2 cut(s) 48, 524
BseMI GCAATG 1 cut(s) 68
BseNI ACTGG 2 cut(s) 699, 729
BseXI GCAGC 5 cut(s) 17, 446, 526, 563, 701
BshFI GGCC 1 cut(s) 564
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 105
BslI CCNNNNNNNGG 2 cut(s) 48, 524
BsmAI GTCTC 2 cut(s) 16, 41
BsnI GGCC 1 cut(s) 564
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 154
Bsp19I CCATGG 1 cut(s) 250
BspACI CCGC 2 cut(s) 65, 419
BspANI GGCC 1 cut(s) 564
BspLI GGNNCC 2 cut(s) 97, 355
BspT107I GGYRCC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 154
BsrI ACTGG 2 cut(s) 699, 729
BssECI CCNNGG 3 cut(s) 209, 235, 250
BssT1I CCWWGG 2 cut(s) 209, 250
Bst2UI CCWGG 2 cut(s) 237, 621
Bst4CI ACNGT 1 cut(s) 667
Bst6I CTCTTC 1 cut(s) 545
BstAUI TGTACA 1 cut(s) 154
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 1 cut(s) 506
BstDSI CCRYGG 1 cut(s) 250
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 2 cut(s) 219, 577
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 1 cut(s) 340
BstMAI GTCTC 2 cut(s) 16, 41
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNI CCWGG 2 cut(s) 237, 621
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 3 cut(s) 103, 235, 619
BstV1I GCAGC 5 cut(s) 17, 446, 526, 563, 701
BstV2I GAAGAC 1 cut(s) 725
BstXI CCANNNNNNTGG 1 cut(s) 572
BsuRI GGCC 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 250
BtrI CACGTC 1 cut(s) 634
BtsCI GGATG 2 cut(s) 219, 577
BtsIMutI CAGTG 2 cut(s) 692, 722
Cac8I GCNNGC 1 cut(s) 56
CfoI GCGC 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 425, 562
CsiI ACCWGGT 1 cut(s) 619
Csp6I GTAC 7 cut(s) 96, 155, 247, 368, 449, 624, 663
CviAII CATG 3 cut(s) 251, 400, 530
CviQI GTAC 7 cut(s) 96, 155, 247, 368, 449, 624, 663
DdeI CTNAG 1 cut(s) 506
DraI TTTAAA 1 cut(s) 193
DraIII CACNNNGTG 1 cut(s) 793
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Ecl136II GAGCTC 1 cut(s) 54
Eco130I CCWWGG 2 cut(s) 209, 250
Eco24I GRGCYC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 230
EcoRII CCWGG 2 cut(s) 235, 619
EcoT14I CCWWGG 2 cut(s) 209, 250
EcoT38I GRGCYC 1 cut(s) 56
ErhI CCWWGG 2 cut(s) 209, 250
FaeI CATG 3 cut(s) 254, 403, 533
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FatI CATG 3 cut(s) 250, 399, 529
Fnu4HI GCNGC 5 cut(s) 6, 435, 515, 577, 690
FokI GGATG 2 cut(s) 226, 584
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 5 cut(s) 6, 435, 515, 577, 690
FspBI CTAG 3 cut(s) 33, 258, 756
GlaI GCGC 1 cut(s) 339
GluI GCNGC 5 cut(s) 6, 435, 515, 577, 690
HaeII RGCGCY 1 cut(s) 341
HaeIII GGCC 1 cut(s) 564
HapII CCGG 1 cut(s) 105
HhaI GCGC 1 cut(s) 340
Hin1II CATG 3 cut(s) 254, 403, 533
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HindIII AAGCTT 1 cut(s) 159
HpaII CCGG 1 cut(s) 105
HphI GGTGA 3 cut(s) 54, 374, 394
Hpy188I TCNGA 2 cut(s) 123, 681
Hpy188III TCNNGA 1 cut(s) 569
HpyCH4III ACNGT 1 cut(s) 667
HpyCH4IV ACGT 2 cut(s) 457, 633
HpyCH4V TGCA 3 cut(s) 434, 514, 689
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpyF3I CTNAG 1 cut(s) 506
HpySE526I ACGT 2 cut(s) 457, 633
Hsp92II CATG 3 cut(s) 254, 403, 533
HspAI GCGC 1 cut(s) 338
KpnI GGTACC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 51
Lsp1109I GCAGC 5 cut(s) 17, 446, 526, 563, 701
LweI GCATC 1 cut(s) 676
MabI ACCWGGT 1 cut(s) 619
MaeI CTAG 3 cut(s) 33, 258, 756
MaeII ACGT 2 cut(s) 457, 633
MaeIII GTNAC 2 cut(s) 60, 230
MboII GAAGA 4 cut(s) 95, 562, 638, 730
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 4 cut(s) 324, 403, 656, 763
MmeI TCCRAC 3 cut(s) 202, 325, 403
MnlI CCTC 8 cut(s) 34, 84, 399, 462, 518, 600, 703, 762
MroXI GAANNNNTTC 1 cut(s) 352
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 3 cut(s) 314, 543, 570
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 3 cut(s) 105, 237, 621
MvaI CCWGG 2 cut(s) 237, 621
MwoI GCNNNNNNNGC 1 cut(s) 64
NciI CCSGG 1 cut(s) 105
NcoI CCATGG 1 cut(s) 250
NlaIII CATG 3 cut(s) 254, 403, 533
NlaIV GGNNCC 2 cut(s) 97, 355
NmuCI GTSAC 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 352
PflMI CCANNNNNTGG 1 cut(s) 48
PkrI GCNGC 5 cut(s) 7, 436, 516, 578, 691
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 235, 619
PspEI GGTNACC 1 cut(s) 230
PspGI CCWGG 2 cut(s) 235, 619
PspN4I GGNNCC 2 cut(s) 97, 355
PspPI GGNCC 2 cut(s) 425, 562
RsaI GTAC 7 cut(s) 97, 156, 248, 369, 450, 625, 664
RsaNI GTAC 7 cut(s) 96, 155, 247, 368, 449, 624, 663
RseI CAYNNNNRTG 3 cut(s) 314, 543, 570
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 5 cut(s) 6, 435, 515, 577, 690
Sau96I GGNCC 2 cut(s) 425, 562
ScrFI CCNGG 3 cut(s) 105, 237, 621
SduI GDGCHC 1 cut(s) 56
SetI ASST 9 cut(s) 56, 91, 163, 355, 454, 460, 607, 622, 636
SexAI ACCWGGT 1 cut(s) 619
SfaNI GCATC 1 cut(s) 676
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 3 cut(s) 314, 543, 570
SpeI ACTAGT 2 cut(s) 32, 755
Sse9I AATT 4 cut(s) 324, 403, 656, 763
SsiI CCGC 2 cut(s) 65, 419
SspMI CTAG 3 cut(s) 33, 258, 756
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 3 cut(s) 103, 235, 619
StyI CCWWGG 2 cut(s) 209, 250
TaaI ACNGT 1 cut(s) 667
TaiI ACGT 2 cut(s) 460, 636
TaqI TCGA 1 cut(s) 76
TasI AATT 4 cut(s) 324, 403, 656, 763
TatI WGTACW 2 cut(s) 154, 662
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 2 cut(s) 699, 729
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 5 cut(s) 5, 434, 514, 576, 689
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 2 cut(s) 332, 416
TspRI CASTG 2 cut(s) 699, 729
Van91I CCANNNNNTGG 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 425
XapI RAATTY 1 cut(s) 656
XmnI GAANNNNTTC 1 cut(s) 352
XspI CTAG 3 cut(s) 33, 258, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.