RchiOBHm_Chr2g0119421

Chloroplastic group IIB intron splicing facilitator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003153
Physical Location & Seq
Forward (+)
31788527 .. 31791687
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49213

Sequence Viewer

Length: 492 bp
ATGTTTTGTCCTGGTTTTGTGCATGTAAACGTGTTGCATCTATCATATCCACGAAGACCCGGTAGAACTCGTTTTCTGAATAAGTGTTCAGTTTCAACAAGTTTTTCTGTACAAGCTTCCTTGCCGGAGAATAATGATAGATTTAAAGTGGAATACACTCCTTGGATGATTGTTGGATTGGGTAACCCTGGAAATAAGTACCATGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAATGCAGGGCATTGTGATGAATACAATTCAGTCAAAGGCACTGGTTGGAATAGGTTCCATTGGAGAGGTACCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCGGTTGGACCACTTGCAGCATATTATCAAGTACCTCTACATCATATATTATTGGTTTATGATGAGATGATCCTACCAAATGGTGTTTCAAGATTGCAGCCAAAAGGAGGACATGAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004045 GO:0016787 GO:0016788 GO:0052689 GO:0140098 GO:0140101
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pept_tRNA_hydro

Protein Analysis

163

Amino Acids

18.08

Weight (kDa)

9.14

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 56 - 162 3.4e-34 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 319
AccB1I GGYRCC 1 cut(s) 319
AciI CCGC 1 cut(s) 371
AclWI GGATC 1 cut(s) 433
AfaI GTAC 4 cut(s) 111, 200, 321, 402
AfiI CCNNNNNNNGG 1 cut(s) 476
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 3 cut(s) 96, 230, 459
AjnI CCWGG 2 cut(s) 10, 187
AjuI GAANNNNNNNTTGG 2 cut(s) 439, 471
AluBI AGCT 2 cut(s) 116, 347
AluI AGCT 2 cut(s) 116, 347
AlwI GGATC 1 cut(s) 433
ApeKI GCWGC 2 cut(s) 386, 466
Asp700I GAANNNNTTC 1 cut(s) 304
Asp718I GGTACC 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 377
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 346
AvaII GGWCC 1 cut(s) 377
BanI GGYRCC 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 61
BbvI GCAGC 2 cut(s) 398, 478
BciT130I CCWGG 2 cut(s) 12, 189
BcnI CCSGG 1 cut(s) 60
BfaI CTAG 2 cut(s) 210, 490
BisI GCNGC 2 cut(s) 387, 467
BlsI GCNGC 2 cut(s) 388, 468
Bme1390I CCNGG 3 cut(s) 12, 60, 189
Bme18I GGWCC 1 cut(s) 377
BmgT120I GGNCC 1 cut(s) 377
BmiI GGNNCC 2 cut(s) 307, 321
BmrFI CCNGG 3 cut(s) 12, 60, 189
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 60
BsaJI CCNNGG 3 cut(s) 161, 187, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 476
Bse1I ACTGG 1 cut(s) 297
BseBI CCWGG 2 cut(s) 12, 189
BseDI CCNNGG 3 cut(s) 161, 187, 202
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 476
BseNI ACTGG 1 cut(s) 297
BseXI GCAGC 2 cut(s) 398, 478
BshNI GGYRCC 1 cut(s) 319
BsiSI CCGG 2 cut(s) 60, 125
BslI CCNNNNNNNGG 1 cut(s) 476
Bsp1407I TGTACA 1 cut(s) 109
Bsp143I GATC 1 cut(s) 438
Bsp19I CCATGG 1 cut(s) 202
BspACI CCGC 1 cut(s) 371
BspLI GGNNCC 2 cut(s) 307, 321
BspPI GGATC 1 cut(s) 433
BspT107I GGYRCC 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 109
BsrI ACTGG 1 cut(s) 297
BssECI CCNNGG 3 cut(s) 161, 187, 202
BssMI GATC 1 cut(s) 438
BssT1I CCWWGG 2 cut(s) 161, 202
Bst2UI CCWGG 2 cut(s) 12, 189
BstAUI TGTACA 1 cut(s) 109
BstDSI CCRYGG 1 cut(s) 202
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 1 cut(s) 171
BstKTI GATC 1 cut(s) 441
BstMBI GATC 1 cut(s) 438
BstNI CCWGG 2 cut(s) 12, 189
BstNSI RCATGY 1 cut(s) 26
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 3 cut(s) 10, 58, 187
BstV1I GCAGC 2 cut(s) 398, 478
BstV2I GAAGAC 1 cut(s) 61
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 1 cut(s) 171
BtsIMutI CAGTG 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 377
Csp6I GTAC 4 cut(s) 110, 199, 320, 401
CspCI CAANNNNNGTGG 2 cut(s) 312, 347
CviAII CATG 4 cut(s) 23, 203, 352, 482
CviJI RGCY 4 cut(s) 116, 340, 347, 469
CviKI_1 RGCY 4 cut(s) 116, 340, 347, 469
CviQI GTAC 4 cut(s) 110, 199, 320, 401
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
DraI TTTAAA 1 cut(s) 145
Eco130I CCWWGG 2 cut(s) 161, 202
Eco47I GGWCC 1 cut(s) 377
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 10, 187
EcoT14I CCWWGG 2 cut(s) 161, 202
ErhI CCWWGG 2 cut(s) 161, 202
FaeI CATG 4 cut(s) 26, 206, 355, 485
FatI CATG 4 cut(s) 22, 202, 351, 481
Fnu4HI GCNGC 2 cut(s) 387, 467
FokI GGATG 1 cut(s) 178
Fsp4HI GCNGC 2 cut(s) 387, 467
FspBI CTAG 2 cut(s) 210, 490
GluI GCNGC 2 cut(s) 387, 467
HapII CCGG 2 cut(s) 60, 125
Hin1II CATG 4 cut(s) 26, 206, 355, 485
HindIII AAGCTT 2 cut(s) 114, 345
HpaII CCGG 2 cut(s) 60, 125
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 1 cut(s) 78
Hpy188III TCNNGA 1 cut(s) 459
Hpy8I GTNNAC 1 cut(s) 28
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 5 cut(s) 22, 37, 256, 386, 466
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 4 cut(s) 26, 206, 355, 485
KpnI GGTACC 1 cut(s) 323
Kzo9I GATC 1 cut(s) 438
LpnPI CCDG 7 cut(s) 24, 73, 138, 174, 201, 242, 278
Lsp1109I GCAGC 2 cut(s) 398, 478
LweI GCATC 1 cut(s) 46
MaeI CTAG 2 cut(s) 210, 490
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 182
MalI GATC 1 cut(s) 440
MboI GATC 1 cut(s) 438
MboII GAAGA 1 cut(s) 66
MluCI AATT 3 cut(s) 276, 324, 355
MmeI TCCRAC 3 cut(s) 154, 277, 355
MnlI CCTC 4 cut(s) 310, 351, 414, 470
MroXI GAANNNNTTC 1 cut(s) 304
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 1 cut(s) 266
MspI CCGG 2 cut(s) 60, 125
MspR9I CCNGG 3 cut(s) 12, 60, 189
MvaI CCWGG 2 cut(s) 12, 189
NciI CCSGG 1 cut(s) 60
NcoI CCATGG 1 cut(s) 202
NdeII GATC 1 cut(s) 438
NlaIII CATG 4 cut(s) 26, 206, 355, 485
NlaIV GGNNCC 2 cut(s) 307, 321
NspI RCATGY 1 cut(s) 26
PdmI GAANNNNTTC 1 cut(s) 304
PkrI GCNGC 2 cut(s) 388, 468
Psp6I CCWGG 2 cut(s) 10, 187
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 2 cut(s) 10, 187
PspN4I GGNNCC 2 cut(s) 307, 321
PspPI GGNCC 1 cut(s) 377
RsaI GTAC 4 cut(s) 111, 200, 321, 402
RsaNI GTAC 4 cut(s) 110, 199, 320, 401
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 2 cut(s) 387, 467
Sau3AI GATC 1 cut(s) 438
Sau96I GGNCC 1 cut(s) 377
ScrFI CCNGG 3 cut(s) 12, 60, 189
SetI ASST 6 cut(s) 33, 118, 307, 321, 349, 406
SfaNI GCATC 1 cut(s) 46
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
Sse9I AATT 3 cut(s) 276, 324, 355
SsiI CCGC 1 cut(s) 371
SspMI CTAG 2 cut(s) 210, 490
StyD4I CCNGG 3 cut(s) 10, 58, 187
StyI CCWWGG 2 cut(s) 161, 202
TaiI ACGT 1 cut(s) 33
TasI AATT 3 cut(s) 276, 324, 355
TatI WGTACW 1 cut(s) 109
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 1 cut(s) 297
TseI GCWGC 2 cut(s) 386, 466
TspDTI ATGAA 2 cut(s) 284, 368
TspRI CASTG 1 cut(s) 297
VpaK11BI GGWCC 1 cut(s) 377
XceI RCATGY 1 cut(s) 26
XmnI GAANNNNTTC 1 cut(s) 304
XspI CTAG 2 cut(s) 210, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.