AT5G19860

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006472
Physical Location & Seq
Reverse (-)
6713953 .. 6715918
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G19860.1

Sequence Viewer

Length: 546 bp
ATGGCCCTTTATCCTTTCTTCATCTCAATCTTCATCTTCTCTTTGACCCTCTTCACTACGACGACCCATTCCCTCAACGAACCCGACCCGGATTCGATTTCCACCGTTTACGAACTCCTCCCAAAGTACGGTCTCCCAAGCGGTCTCTTACCGGACACCGTCACCGATTTCACCCTCTCCGACGATGGCCGATTCGTCGTTCACTTGCCCAATTCCTGTGAGATTGAATTTGACTACCTCGTTCACTACGATAAGACCATATCTGGTCGTATCGGTTACGGTTCAATCACCGAGCTCAAGGGTATTCAGGTGAAGAAATTCTTTATTTGGCTCGATGTCGATGAGATCAAGGTCGATCTTCCACCTTCTGATTCAATCTACTTCAAAGTTGGCTTTATCAATAAGAAGCTTGATATTGACCAGTTTAAGACTATACATTCTTGTCACGATAATGGTGTCTCTGGCTCTTGTGGAGATTCATGGAAGAGTTTTCTCGAGAAAGGGCAAGGAATGATGGATGAAGCGGAAATGTTAATCACCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

181

Amino Acids

20.44

Weight (kDa)

4.62

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 36 - 143 2.2e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 141, 524
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 2 cut(s) 227, 317
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 128
AgsI TTSAA 4 cut(s) 227, 285, 375, 385
AluBI AGCT 2 cut(s) 295, 409
AluI AGCT 2 cut(s) 295, 409
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 3 cut(s) 137, 149, 463
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 2 cut(s) 3, 187
ApoI RAATTY 2 cut(s) 227, 317
Asp700I GAANNNNTTC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 5 cut(s) 154, 163, 280, 322, 529
AvaI CYCGRG 1 cut(s) 494
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 297
BccI CCATC 2 cut(s) 179, 508
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 3 cut(s) 137, 149, 463
Bme1390I CCNGG 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 89
BsaI GGTCTC 2 cut(s) 137, 149
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 421
BseGI GGATG 1 cut(s) 523
BseLI CCNNNNNNNGG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 1 cut(s) 107
BshFI GGCC 2 cut(s) 5, 189
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 2 cut(s) 89, 152
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 3 cut(s) 137, 149, 463
BsnI GGCC 2 cut(s) 5, 189
Bso31I GGTCTC 2 cut(s) 137, 149
BsoBI CYCGRG 1 cut(s) 494
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 2 cut(s) 345, 355
BspACI CCGC 2 cut(s) 141, 524
BspANI GGCC 2 cut(s) 5, 189
BspTNI GGTCTC 2 cut(s) 137, 149
BsrI ACTGG 1 cut(s) 421
BssMI GATC 2 cut(s) 345, 355
Bst4CI ACNGT 4 cut(s) 106, 131, 160, 281
Bst6I CTCTTC 2 cut(s) 56, 479
BstF5I GGATG 1 cut(s) 523
BstKTI GATC 2 cut(s) 348, 358
BstMAI GTCTC 3 cut(s) 137, 149, 463
BstMBI GATC 2 cut(s) 345, 355
BstSCI CCNGG 1 cut(s) 87
BsuRI GGCC 2 cut(s) 5, 189
BtsCI GGATG 1 cut(s) 523
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 127
CviAII CATG 1 cut(s) 480
CviJI RGCY 7 cut(s) 5, 189, 295, 331, 393, 409, 465
CviKI_1 RGCY 7 cut(s) 5, 189, 295, 331, 393, 409, 465
CviQI GTAC 1 cut(s) 127
DpnI GATC 2 cut(s) 347, 357
DpnII GATC 2 cut(s) 345, 355
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 2 cut(s) 56, 479
EarI CTCTTC 2 cut(s) 56, 479
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 297
Eco31I GGTCTC 2 cut(s) 137, 149
Eco53kI GAGCTC 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 494
EcoICRI GAGCTC 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 1 cut(s) 483
FaiI YATR 3 cut(s) 260, 434, 481
FalI AAGNNNNNCTT 2 cut(s) 305, 337
FatI CATG 1 cut(s) 479
FokI GGATG 1 cut(s) 530
FriOI GRGCYC 1 cut(s) 297
HaeIII GGCC 2 cut(s) 5, 189
HapII CCGG 2 cut(s) 89, 152
Hin1II CATG 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 4 cut(s) 92, 192, 371, 476
HpaII CCGG 2 cut(s) 89, 152
HphI GGTGA 5 cut(s) 154, 163, 280, 322, 529
Hpy166II GTNNAC 3 cut(s) 109, 202, 244
Hpy188I TCNGA 2 cut(s) 181, 370
Hpy188III TCNNGA 3 cut(s) 446, 494, 496
Hpy8I GTNNAC 3 cut(s) 109, 202, 244
Hpy99I CGWCG 3 cut(s) 64, 185, 200
HpyAV CCTTC 1 cut(s) 375
HpyCH4III ACNGT 4 cut(s) 106, 131, 160, 281
Hsp92II CATG 1 cut(s) 483
Kzo9I GATC 2 cut(s) 345, 355
LpnPI CCDG 7 cut(s) 102, 165, 229, 249, 293, 434, 447
MaeIII GTNAC 3 cut(s) 160, 275, 443
MalI GATC 2 cut(s) 347, 357
MboI GATC 2 cut(s) 345, 355
MboII GAAGA 7 cut(s) 10, 22, 28, 43, 325, 350, 496
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 3 cut(s) 211, 227, 317
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 5 cut(s) 59, 83, 128, 185, 248
MroXI GAANNNNTTC 1 cut(s) 317
MseI TTAA 2 cut(s) 426, 533
MslI CAYNNNNRTG 1 cut(s) 450
MspI CCGG 2 cut(s) 89, 152
MspR9I CCNGG 1 cut(s) 89
NciI CCSGG 1 cut(s) 89
NdeII GATC 2 cut(s) 345, 355
NlaIII CATG 1 cut(s) 483
NmuCI GTSAC 2 cut(s) 160, 443
PaeR7I CTCGAG 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 317
PfeI GAWTC 4 cut(s) 92, 192, 371, 476
PflFI GACNNNGTC 1 cut(s) 158
Psp124BI GAGCTC 1 cut(s) 297
PspPI GGNCC 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 158
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 450
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 426, 533
Sau3AI GATC 2 cut(s) 345, 355
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 297
SetI ASST 6 cut(s) 240, 297, 312, 354, 367, 411
Sfr274I CTCGAG 1 cut(s) 494
SlaI CTCGAG 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 450
SmlI CTYRAG 2 cut(s) 296, 494
SmoI CTYRAG 2 cut(s) 296, 494
Sse9I AATT 3 cut(s) 211, 227, 317
SsiI CCGC 2 cut(s) 141, 524
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 4 cut(s) 106, 131, 160, 281
TaqI TCGA 5 cut(s) 95, 333, 339, 354, 495
TasI AATT 3 cut(s) 211, 227, 317
TfiI GAWTC 4 cut(s) 92, 192, 371, 476
Tru1I TTAA 2 cut(s) 426, 533
Tru9I TTAA 2 cut(s) 426, 533
TseFI GTSAC 2 cut(s) 160, 443
Tsp45I GTSAC 2 cut(s) 160, 443
TspDTI ATGAA 4 cut(s) 10, 22, 468, 534
Tth111I GACNNNGTC 1 cut(s) 158
XapI RAATTY 2 cut(s) 227, 317
XhoI CTCGAG 1 cut(s) 494
XmnI GAANNNNTTC 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.