Rh5BG553500

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006472
Physical Location & Seq
Forward (+)
87891651 .. 87893515
1865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG553500.1

Sequence Viewer

Length: 570 bp
ATGGCTCCATTTCCAAAACCCCATCCGGGTCTAATCCCAATCCTCCTCCTCCTCCTCCTCTCCACCGCCGCAAGCCCCTCTCTTTCTCTCTCCTCCGACTCCTCCGCCTCGGTCTTCGAGCTCTTACCCAAATATGGGCTCCCCAGTGGGCTCCTACCCGCCTCGGTTTCCAACTACACGTTGTCCGCCGACGACGGCCGATTCGTCGTCGTTTTGGAGAAGCCGTGCTATATCCAGTTCGAGTACTTGGTGTACTACGAGACCCGCATCACCGGAAAGCTGAGCTACGGCGCGATCACGGAGCTGAAGGGCATTCAGGTCCAAAGATTGTTCTTTTGGTTCGACGTCGATGAGATCAGGGTCGATTTGCCGCCGTCCGATAGTATTTACTTCACGGTTGGGATCATCAACAAGAAGCTCGATGTCGATCAGTTCCAGAGCGTTCATTCTTGCCGCGACGGGCTTTCGGGTTCGGGTTCGGGTTCGGGTTCTTGCCTCGGGTCGTTGAAACGGGTCGTTCAGCTTCCATCTCCACTGGAAGAAGAGATAAAGATGCTACTTACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

189

Amino Acids

20.66

Weight (kDa)

5.05

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 40 - 146 4.7e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccII CGCG 2 cut(s) 293, 456
AciI CCGC 8 cut(s) 66, 69, 105, 159, 186, 265, 371, 454
AclWI GGATC 1 cut(s) 410
AcoI YGGCCR 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 326
AcyI GRCGYC 1 cut(s) 345
AfaI GTAC 2 cut(s) 245, 254
AfiI CCNNNNNNNGG 3 cut(s) 26, 134, 135
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 1 cut(s) 508
AluBI AGCT 6 cut(s) 121, 280, 285, 304, 418, 523
AluI AGCT 6 cut(s) 121, 280, 285, 304, 418, 523
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 254
AlwI GGATC 1 cut(s) 410
Ama87I CYCGRG 1 cut(s) 497
AoxI GGCC 1 cut(s) 196
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 1 cut(s) 319
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 1 cut(s) 262
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 1 cut(s) 319
BanII GRGCYC 3 cut(s) 123, 141, 153
BbsI GAAGAC 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 123
BccI CCATC 2 cut(s) 30, 535
BceAI ACGGC 4 cut(s) 208, 211, 304, 358
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 254
BisI GCNGC 3 cut(s) 69, 371, 454
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 3 cut(s) 70, 372, 455
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 1 cut(s) 319
BmeT110I CYCGRG 1 cut(s) 497
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 3 cut(s) 6, 140, 152
BmrFI CCNGG 1 cut(s) 27
BmrI ACTGGG 1 cut(s) 138
BmsI GCATC 2 cut(s) 276, 543
BmuI ACTGGG 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 106
Bpu1102I GCTNAGC 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 3 cut(s) 108, 162, 496
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 134, 135
Bse1I ACTGG 3 cut(s) 144, 235, 540
Bse8I GATNNNNATC 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 108, 162, 496
BseGI GGATG 1 cut(s) 22
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 3 cut(s) 26, 134, 135
BseMII CTCAG 2 cut(s) 272, 555
BseNI ACTGG 3 cut(s) 144, 235, 540
BseRI GAGGAG 7 cut(s) 35, 38, 41, 44, 47, 82, 91
BseX3I CGGCCG 1 cut(s) 196
Bsh1236I CGCG 2 cut(s) 293, 456
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 1 cut(s) 198
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 497
BsiSI CCGG 2 cut(s) 26, 273
BslI CCNNNNNNNGG 3 cut(s) 26, 134, 135
BsmAI GTCTC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 497
Bsp1286I GDGCHC 3 cut(s) 123, 141, 153
Bsp143I GATC 4 cut(s) 294, 354, 402, 427
Bsp1720I GCTNAGC 1 cut(s) 281
BspACI CCGC 8 cut(s) 66, 69, 105, 159, 186, 265, 371, 454
BspANI GGCC 1 cut(s) 198
BspCNI CTCAG 2 cut(s) 273, 556
BspFNI CGCG 2 cut(s) 293, 456
BspLI GGNNCC 3 cut(s) 6, 140, 152
BspPI GGATC 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 254
BsrI ACTGG 3 cut(s) 144, 235, 540
BssECI CCNNGG 3 cut(s) 108, 162, 496
BssMI GATC 4 cut(s) 294, 354, 402, 427
BssNI GRCGYC 1 cut(s) 345
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 1 cut(s) 537
BstACI GRCGYC 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 2 cut(s) 281, 564
BstF5I GGATG 1 cut(s) 22
BstFNI CGCG 2 cut(s) 293, 456
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 4 cut(s) 297, 357, 405, 430
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 4 cut(s) 294, 354, 402, 427
BstMCI CGRYCG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 25
BstUI CGCG 2 cut(s) 293, 456
BstV2I GAAGAC 1 cut(s) 106
BstZI CGGCCG 1 cut(s) 196
BsuRI GGCC 1 cut(s) 198
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 151, 533
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 319
Csp6I GTAC 2 cut(s) 244, 253
CviQI GTAC 2 cut(s) 244, 253
DdeI CTNAG 2 cut(s) 281, 564
DpnI GATC 4 cut(s) 296, 356, 404, 429
DpnII GATC 4 cut(s) 294, 354, 402, 427
EaeI YGGCCR 1 cut(s) 196
EagI CGGCCG 1 cut(s) 196
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
EciI GGCGGA 2 cut(s) 94, 175
Ecl136II GAGCTC 1 cut(s) 121
EclXI CGGCCG 1 cut(s) 196
Eco24I GRGCYC 3 cut(s) 123, 141, 153
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 319
Eco52I CGGCCG 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 497
EcoICRI GAGCTC 1 cut(s) 121
EcoT38I GRGCYC 3 cut(s) 123, 141, 153
FaiI YATR 2 cut(s) 135, 231
FauI CCCGC 2 cut(s) 166, 272
Fnu4HI GCNGC 3 cut(s) 69, 371, 454
FokI GGATG 1 cut(s) 9
FriOI GRGCYC 3 cut(s) 123, 141, 153
Fsp4HI GCNGC 3 cut(s) 69, 371, 454
GlaI GCGC 1 cut(s) 292
GluI GCNGC 3 cut(s) 69, 371, 454
HaeIII GGCC 1 cut(s) 198
HapII CCGG 2 cut(s) 26, 273
HhaI GCGC 1 cut(s) 293
Hin1I GRCGYC 1 cut(s) 345
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HinfI GANTC 2 cut(s) 98, 201
HpaII CCGG 2 cut(s) 26, 273
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 2 cut(s) 97, 379
Hpy188III TCNNGA 1 cut(s) 436
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 7 cut(s) 194, 197, 209, 212, 347, 350, 461
HpyAV CCTTC 1 cut(s) 301
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 2 cut(s) 179, 345
HpyF3I CTNAG 2 cut(s) 281, 564
HpySE526I ACGT 2 cut(s) 179, 345
Hsp92I GRCGYC 1 cut(s) 345
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 4 cut(s) 294, 354, 402, 427
LmnI GCTCC 4 cut(s) 10, 144, 156, 301
LpnPI CCDG 8 cut(s) 39, 157, 248, 286, 302, 343, 449, 521
LweI GCATC 2 cut(s) 276, 543
MaeII ACGT 2 cut(s) 179, 345
MalI GATC 4 cut(s) 296, 356, 404, 429
MboI GATC 4 cut(s) 294, 354, 402, 427
MboII GAAGA 3 cut(s) 106, 551, 554
MhlI GDGCHC 3 cut(s) 123, 141, 153
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 2 cut(s) 120, 195
MspI CCGG 2 cut(s) 26, 273
MspR9I CCNGG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 312
MvnI CGCG 2 cut(s) 293, 456
NciI CCSGG 1 cut(s) 27
NdeII GATC 4 cut(s) 294, 354, 402, 427
NlaIV GGNNCC 3 cut(s) 6, 140, 152
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 201
PkrI GCNGC 3 cut(s) 70, 372, 455
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Psp124BI GAGCTC 1 cut(s) 123
PspN4I GGNNCC 3 cut(s) 6, 140, 152
PspPI GGNCC 1 cut(s) 319
RsaI GTAC 2 cut(s) 245, 254
RsaNI GTAC 2 cut(s) 244, 253
SacI GAGCTC 1 cut(s) 123
SatI GCNGC 3 cut(s) 69, 371, 454
Sau3AI GATC 4 cut(s) 294, 354, 402, 427
Sau96I GGNCC 1 cut(s) 319
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 3 cut(s) 123, 141, 153
SetI ASST 9 cut(s) 123, 182, 282, 287, 306, 321, 348, 420, 525
SfaNI GCATC 2 cut(s) 276, 543
SinI GGWCC 1 cut(s) 319
SsiI CCGC 8 cut(s) 66, 69, 105, 159, 186, 265, 371, 454
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 25
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 2 cut(s) 182, 348
TaqI TCGA 7 cut(s) 117, 240, 342, 348, 363, 420, 426
TaqII GACCGA 1 cut(s) 100
TatI WGTACW 2 cut(s) 243, 252
TauI GCSGC 3 cut(s) 71, 373, 456
TfiI GAWTC 1 cut(s) 201
TscAI CASTG 2 cut(s) 151, 540
TspDTI ATGAA 1 cut(s) 434
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 2 cut(s) 151, 540
VpaK11BI GGWCC 1 cut(s) 319
ZraI GACGTC 1 cut(s) 346
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.