RchiOBHm_Chr5g0081571

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006472
Physical Location & Seq
Forward (+)
87534289 .. 87538548
4260 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35582

Sequence Viewer

Length: 564 bp
ATGGCTCCATTTCCAAAACCCCATCCGGGTCTAATCCCAATCCTCCTCCTCCTCCTCCTCTCCACCGCCGCAAGCCCCTCTCTTTCTCTCTCCTCCGACTCCTCCGCCTCGGTCTTCGAGCTCTTACCCAAATATGGGCTCCCCAGTGGGCTCCTACCCGCCTCGGTTTCCAACTACACGTTGTCCGCCGACGACGGCCGATTCGTCGTCGTTTTGGAGAAGCCGTGCTATATCCAGTTCGAGTACTTGGTGTACTACGAGACCCGCATCACCGGAAAGCTGAGCTACGGCGCGATCACGGAGCTGAAGGGCATTCAGGTCCAAAGATTGTTCTTTTGGTTCGACGTCGACGAGATCAGGGTCGATTTGCCGCCGTCCGATAGTATTTACTTCACCGTTGGGATCATCAACAAGAAGCTCGATGTCGATCAGTTCCAGAGCGTTCATTCTTGCCGCGACGGGCTTTCGGGTTCGGGTTCGGGTTCTTGCCTCGGGTCCTTGAAACGGGTCATTCAGCTTCCATCTCCGCTGGAAGAAGAGATAAAGATGCTACTTACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

187

Amino Acids

20.53

Weight (kDa)

5.05

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 40 - 146 4.6e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccI GTMKAC 1 cut(s) 348
AccII CGCG 2 cut(s) 293, 456
AciI CCGC 9 cut(s) 66, 69, 105, 159, 186, 265, 371, 454, 527
AclWI GGATC 1 cut(s) 410
AcoI YGGCCR 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 326
AcyI GRCGYC 1 cut(s) 345
AfaI GTAC 2 cut(s) 245, 254
AfiI CCNNNNNNNGG 4 cut(s) 26, 134, 135, 504
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 1 cut(s) 502
AluBI AGCT 6 cut(s) 121, 280, 285, 304, 418, 517
AluI AGCT 6 cut(s) 121, 280, 285, 304, 418, 517
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 254
AlwI GGATC 1 cut(s) 410
Ama87I CYCGRG 1 cut(s) 491
AoxI GGCC 1 cut(s) 196
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 319, 495
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 262, 385
AvaI CYCGRG 1 cut(s) 491
AvaII GGWCC 2 cut(s) 319, 495
BanII GRGCYC 3 cut(s) 123, 141, 153
BbsI GAAGAC 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 123
BccI CCATC 2 cut(s) 30, 529
BceAI ACGGC 4 cut(s) 208, 211, 304, 358
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 254
BisI GCNGC 3 cut(s) 69, 371, 454
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 3 cut(s) 70, 372, 455
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 2 cut(s) 319, 495
BmeT110I CYCGRG 1 cut(s) 491
BmgT120I GGNCC 2 cut(s) 319, 495
BmiI GGNNCC 4 cut(s) 6, 140, 152, 496
BmrFI CCNGG 1 cut(s) 27
BmrI ACTGGG 1 cut(s) 138
BmsI GCATC 2 cut(s) 276, 537
BmuI ACTGGG 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 106
Bpu1102I GCTNAGC 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 3 cut(s) 108, 162, 490
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 4 cut(s) 26, 134, 135, 504
Bse1I ACTGG 2 cut(s) 144, 235
Bse8I GATNNNNATC 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 108, 162, 490
BseGI GGATG 1 cut(s) 22
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 4 cut(s) 26, 134, 135, 504
BseMII CTCAG 2 cut(s) 272, 549
BseNI ACTGG 2 cut(s) 144, 235
BseRI GAGGAG 7 cut(s) 35, 38, 41, 44, 47, 82, 91
BseX3I CGGCCG 1 cut(s) 196
Bsh1236I CGCG 2 cut(s) 293, 456
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 1 cut(s) 198
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 491
BsiSI CCGG 2 cut(s) 26, 273
BslI CCNNNNNNNGG 4 cut(s) 26, 134, 135, 504
BsmAI GTCTC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 491
Bsp1286I GDGCHC 3 cut(s) 123, 141, 153
Bsp143I GATC 4 cut(s) 294, 354, 402, 427
Bsp1720I GCTNAGC 1 cut(s) 281
BspACI CCGC 9 cut(s) 66, 69, 105, 159, 186, 265, 371, 454, 527
BspANI GGCC 1 cut(s) 198
BspCNI CTCAG 2 cut(s) 273, 550
BspFNI CGCG 2 cut(s) 293, 456
BspLI GGNNCC 4 cut(s) 6, 140, 152, 496
BspPI GGATC 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 254
BsrI ACTGG 2 cut(s) 144, 235
BssECI CCNNGG 3 cut(s) 108, 162, 490
BssMI GATC 4 cut(s) 294, 354, 402, 427
BssNI GRCGYC 1 cut(s) 345
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 1 cut(s) 531
BstACI GRCGYC 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 2 cut(s) 281, 558
BstF5I GGATG 1 cut(s) 22
BstFNI CGCG 2 cut(s) 293, 456
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 4 cut(s) 297, 357, 405, 430
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 4 cut(s) 294, 354, 402, 427
BstMCI CGRYCG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 25
BstUI CGCG 2 cut(s) 293, 456
BstV2I GAAGAC 1 cut(s) 106
BstZI CGGCCG 1 cut(s) 196
BsuRI GGCC 1 cut(s) 198
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 319, 495
Csp6I GTAC 2 cut(s) 244, 253
CviQI GTAC 2 cut(s) 244, 253
DdeI CTNAG 2 cut(s) 281, 558
DpnI GATC 4 cut(s) 296, 356, 404, 429
DpnII GATC 4 cut(s) 294, 354, 402, 427
EaeI YGGCCR 1 cut(s) 196
EagI CGGCCG 1 cut(s) 196
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
EciI GGCGGA 2 cut(s) 94, 175
Ecl136II GAGCTC 1 cut(s) 121
EclXI CGGCCG 1 cut(s) 196
Eco24I GRGCYC 3 cut(s) 123, 141, 153
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 2 cut(s) 319, 495
Eco52I CGGCCG 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 491
EcoICRI GAGCTC 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 495
EcoT38I GRGCYC 3 cut(s) 123, 141, 153
FaiI YATR 2 cut(s) 135, 231
FauI CCCGC 2 cut(s) 166, 272
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 3 cut(s) 69, 371, 454
FokI GGATG 1 cut(s) 9
FriOI GRGCYC 3 cut(s) 123, 141, 153
Fsp4HI GCNGC 3 cut(s) 69, 371, 454
GlaI GCGC 1 cut(s) 292
GluI GCNGC 3 cut(s) 69, 371, 454
HaeIII GGCC 1 cut(s) 198
HapII CCGG 2 cut(s) 26, 273
HhaI GCGC 1 cut(s) 293
Hin1I GRCGYC 1 cut(s) 345
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HinfI GANTC 2 cut(s) 98, 201
HpaII CCGG 2 cut(s) 26, 273
HphI GGTGA 2 cut(s) 262, 385
Hpy166II GTNNAC 2 cut(s) 253, 349
Hpy188I TCNGA 2 cut(s) 97, 379
Hpy188III TCNNGA 1 cut(s) 436
Hpy8I GTNNAC 2 cut(s) 253, 349
Hpy99I CGWCG 8 cut(s) 194, 197, 209, 212, 347, 350, 353, 461
HpyAV CCTTC 1 cut(s) 301
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 2 cut(s) 179, 345
HpyF3I CTNAG 2 cut(s) 281, 558
HpySE526I ACGT 2 cut(s) 179, 345
Hsp92I GRCGYC 1 cut(s) 345
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 4 cut(s) 294, 354, 402, 427
LmnI GCTCC 4 cut(s) 10, 144, 156, 301
LpnPI CCDG 8 cut(s) 39, 157, 248, 286, 302, 343, 449, 515
LweI GCATC 2 cut(s) 276, 537
MaeII ACGT 2 cut(s) 179, 345
MalI GATC 4 cut(s) 296, 356, 404, 429
MboI GATC 4 cut(s) 294, 354, 402, 427
MboII GAAGA 3 cut(s) 106, 545, 548
MhlI GDGCHC 3 cut(s) 123, 141, 153
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 2 cut(s) 120, 195
MspA1I CMGCKG 1 cut(s) 529
MspI CCGG 2 cut(s) 26, 273
MspR9I CCNGG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 312
MvnI CGCG 2 cut(s) 293, 456
NciI CCSGG 1 cut(s) 27
NdeII GATC 4 cut(s) 294, 354, 402, 427
NlaIV GGNNCC 4 cut(s) 6, 140, 152, 496
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 348
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 201
PkrI GCNGC 3 cut(s) 70, 372, 455
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 495
PspN4I GGNNCC 4 cut(s) 6, 140, 152, 496
PspPI GGNCC 2 cut(s) 319, 495
PspPPI RGGWCCY 1 cut(s) 495
RsaI GTAC 2 cut(s) 245, 254
RsaNI GTAC 2 cut(s) 244, 253
SacI GAGCTC 1 cut(s) 123
SalI GTCGAC 1 cut(s) 347
SatI GCNGC 3 cut(s) 69, 371, 454
Sau3AI GATC 4 cut(s) 294, 354, 402, 427
Sau96I GGNCC 2 cut(s) 319, 495
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 3 cut(s) 123, 141, 153
SetI ASST 9 cut(s) 123, 182, 282, 287, 306, 321, 348, 420, 519
SfaNI GCATC 2 cut(s) 276, 537
SgrDI CGTCGACG 1 cut(s) 347
SinI GGWCC 2 cut(s) 319, 495
SsiI CCGC 9 cut(s) 66, 69, 105, 159, 186, 265, 371, 454, 527
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 25
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 2 cut(s) 182, 348
TaqI TCGA 7 cut(s) 117, 240, 342, 348, 363, 420, 426
TaqII GACCGA 1 cut(s) 100
TatI WGTACW 2 cut(s) 243, 252
TauI GCSGC 3 cut(s) 71, 373, 456
TfiI GAWTC 1 cut(s) 201
TscAI CASTG 1 cut(s) 151
TspDTI ATGAA 1 cut(s) 434
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 2 cut(s) 319, 495
XmiI GTMKAC 1 cut(s) 348
ZraI GACGTC 1 cut(s) 346
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.