FvH4_3g44860

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006472
Physical Location & Seq
Forward (+)
37339970 .. 37342509
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44860.t1

Sequence Viewer

Length: 543 bp
ATGGCTCCATTTCCAAAACCGGGTCTAATCCCAATCCTCCTCCTCCTCCTCCTCCTCTCCGCCGCCCCAAGACTCTCCCTTTCCGACTCCACCGTCTTCGAGCTCCTCCCCAAGTACGGCCTCCCCAGCGGTCTCCTCCCCGCCTCCGTCTCCAACTACACTTTATCCGACGACGGCCGATTCGTCGTCGTTTTGGACAAGCCCTGCTACATCCAGTTCGAGTACTTGGTCTACTACGAGACCAAGATCACCGGAAAACTCAGCTACGGCGCGATCACGGATCTCAAGGGCATTCAGGTCCAAAGACTTTTCTTTTGGTTCGACGTCGATGAGATCCGGGTCGATTTGCCGCCGTCCGATAGCATCTATTTCACGGTTGGGATCATCAACAAGAAGCTCGATGTTGATCAGTTCCAGAGTGTTCATTCTTGCCGAGATGGGCTTTCGGGTTCGGGTTCGGGTTCTTGTCTCGGGTCGTTGAAACGGGTCATTCAGCTTCCGTCTCCACTGGAAGAAGAGATAAAGATGCTACTTACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

181

Amino Acids

19.94

Weight (kDa)

5.04

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 34 - 139 3.2e-32 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AatII GACGTC 1 cut(s) 327
AccI GTMKAC 1 cut(s) 231
AccII CGCG 1 cut(s) 272
AciI CCGC 5 cut(s) 60, 63, 129, 141, 350
AclWI GGATC 3 cut(s) 288, 328, 389
AcoI YGGCCR 1 cut(s) 175
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 2 cut(s) 116, 224
AfiI CCNNNNNNNGG 2 cut(s) 20, 116
AgsI TTSAA 1 cut(s) 481
AluBI AGCT 4 cut(s) 103, 264, 397, 496
AluI AGCT 4 cut(s) 103, 264, 397, 496
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 5 cut(s) 137, 154, 233, 473, 507
AlwI GGATC 3 cut(s) 288, 328, 389
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 2 cut(s) 118, 175
ArsI GACNNNNNNTTYG 4 cut(s) 164, 196, 297, 329
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 2 cut(s) 21, 338
AsuHPI GGTGA 1 cut(s) 241
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 105
BccI CCATC 1 cut(s) 431
BceAI ACGGC 4 cut(s) 133, 190, 283, 337
BclI TGATCA 1 cut(s) 406
BcnI CCSGG 2 cut(s) 21, 338
BcoDI GTCTC 5 cut(s) 137, 154, 233, 473, 507
BisI GCNGC 2 cut(s) 63, 350
BlsI GCNGC 2 cut(s) 64, 351
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 21, 338
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 21, 338
BmsI GCATC 2 cut(s) 372, 516
BpiI GAAGAC 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 2 cut(s) 21, 338
BsaBI GATNNNNATC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 324
BsaI GGTCTC 2 cut(s) 137, 233
BsaWI WCCGGW 1 cut(s) 251
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 116
Bse1I ACTGG 2 cut(s) 214, 513
Bse8I GATNNNNATC 1 cut(s) 405
BseGI GGATG 1 cut(s) 210
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 2 cut(s) 20, 116
BseMII CTCAG 2 cut(s) 274, 528
BseNI ACTGG 2 cut(s) 214, 513
BseRI GAGGAG 8 cut(s) 29, 32, 35, 38, 41, 44, 95, 125
BseX3I CGGCCG 1 cut(s) 175
BseYI CCCAGC 1 cut(s) 125
Bsh1236I CGCG 1 cut(s) 272
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 2 cut(s) 120, 177
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 3 cut(s) 20, 252, 337
BslI CCNNNNNNNGG 2 cut(s) 20, 116
BsmAI GTCTC 5 cut(s) 137, 154, 233, 473, 507
BsmBI CGTCTC 2 cut(s) 154, 507
BsmI GAATGC 1 cut(s) 291
BsnI GGCC 2 cut(s) 120, 177
Bso31I GGTCTC 2 cut(s) 137, 233
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 6 cut(s) 246, 273, 280, 333, 381, 406
BspACI CCGC 5 cut(s) 60, 63, 129, 141, 350
BspANI GGCC 2 cut(s) 120, 177
BspCNI CTCAG 2 cut(s) 273, 529
BspFNI CGCG 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 3 cut(s) 288, 328, 389
BspTNI GGTCTC 2 cut(s) 137, 233
BsrI ACTGG 2 cut(s) 214, 513
BssMI GATC 6 cut(s) 246, 273, 280, 333, 381, 406
BssNI GRCGYC 1 cut(s) 324
Bst4CI ACNGT 2 cut(s) 94, 376
Bst6I CTCTTC 1 cut(s) 510
BstACI GRCGYC 1 cut(s) 324
BstDEI CTNAG 2 cut(s) 260, 537
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 1 cut(s) 272
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 6 cut(s) 249, 276, 283, 336, 384, 409
BstMAI GTCTC 5 cut(s) 137, 154, 233, 473, 507
BstMBI GATC 6 cut(s) 246, 273, 280, 333, 381, 406
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 1 cut(s) 126
BstSCI CCNGG 2 cut(s) 19, 336
BstUI CGCG 1 cut(s) 272
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 280, 333
BstYI RGATCY 2 cut(s) 280, 333
BstZI CGGCCG 1 cut(s) 175
BsuRI GGCC 2 cut(s) 120, 177
BtsCI GGATG 1 cut(s) 210
BtsIMutI CAGTG 1 cut(s) 506
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 115, 223
CviJI RGCY 9 cut(s) 5, 103, 120, 177, 202, 264, 397, 442, 496
CviKI_1 RGCY 9 cut(s) 5, 103, 120, 177, 202, 264, 397, 442, 496
CviQI GTAC 2 cut(s) 115, 223
DdeI CTNAG 2 cut(s) 260, 537
DpnI GATC 6 cut(s) 248, 275, 282, 335, 383, 408
DpnII GATC 6 cut(s) 246, 273, 280, 333, 381, 406
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 175
EagI CGGCCG 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
EciI GGCGGA 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 103
EclXI CGGCCG 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 2 cut(s) 137, 233
Eco47I GGWCC 1 cut(s) 298
Eco52I CGGCCG 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 105
Esp3I CGTCTC 2 cut(s) 154, 507
FauI CCCGC 1 cut(s) 148
FbaI TGATCA 1 cut(s) 406
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 2 cut(s) 63, 350
FokI GGATG 1 cut(s) 197
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 63, 350
GlaI GCGC 1 cut(s) 271
GluI GCNGC 2 cut(s) 63, 350
GsaI CCCAGC 1 cut(s) 129
HaeIII GGCC 2 cut(s) 120, 177
HapII CCGG 3 cut(s) 20, 252, 337
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 1 cut(s) 324
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HinfI GANTC 3 cut(s) 72, 86, 180
HpaII CCGG 3 cut(s) 20, 252, 337
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 3 cut(s) 85, 169, 358
Hpy188III TCNNGA 1 cut(s) 415
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 6 cut(s) 173, 176, 188, 191, 326, 329
HpyCH4III ACNGT 2 cut(s) 94, 376
HpyCH4IV ACGT 1 cut(s) 324
HpyF10VI GCNNNNNNNGC 1 cut(s) 126
HpyF3I CTNAG 2 cut(s) 260, 537
HpySE526I ACGT 1 cut(s) 324
Hsp92I GRCGYC 1 cut(s) 324
HspAI GCGC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 6 cut(s) 246, 273, 280, 333, 381, 406
LmnI GCTCC 2 cut(s) 10, 108
LpnPI CCDG 9 cut(s) 33, 139, 217, 227, 265, 281, 350, 428, 494
LweI GCATC 2 cut(s) 372, 516
MaeII ACGT 1 cut(s) 324
MalI GATC 6 cut(s) 248, 275, 282, 335, 383, 408
MboI GATC 6 cut(s) 246, 273, 280, 333, 381, 406
MboII GAAGA 3 cut(s) 88, 524, 527
MflI RGATCY 2 cut(s) 280, 333
MhlI GDGCHC 1 cut(s) 105
MlyI GAGTC 2 cut(s) 66, 80
MmeI TCCRAC 3 cut(s) 108, 177, 192
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 3 cut(s) 20, 252, 337
MspR9I CCNGG 2 cut(s) 21, 338
Mva1269I GAATGC 1 cut(s) 291
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 1 cut(s) 126
NciI CCSGG 2 cut(s) 21, 338
NdeII GATC 6 cut(s) 246, 273, 280, 333, 381, 406
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 458
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 1 cut(s) 291
PfeI GAWTC 1 cut(s) 180
PkrI GCNGC 2 cut(s) 64, 351
PleI GAGTC 2 cut(s) 66, 80
PpsI GAGTC 2 cut(s) 66, 80
Psp124BI GAGCTC 1 cut(s) 105
PspFI CCCAGC 1 cut(s) 125
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 298
PsuI RGATCY 2 cut(s) 280, 333
RsaI GTAC 2 cut(s) 116, 224
RsaNI GTAC 2 cut(s) 115, 223
SacI GAGCTC 1 cut(s) 105
SatI GCNGC 2 cut(s) 63, 350
Sau3AI GATC 6 cut(s) 246, 273, 280, 333, 381, 406
Sau96I GGNCC 1 cut(s) 298
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 2 cut(s) 66, 80
ScrFI CCNGG 2 cut(s) 21, 338
SduI GDGCHC 1 cut(s) 105
SetI ASST 6 cut(s) 105, 266, 300, 327, 399, 498
SfaNI GCATC 2 cut(s) 372, 516
SinI GGWCC 1 cut(s) 298
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SsiI CCGC 5 cut(s) 60, 63, 129, 141, 350
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 2 cut(s) 19, 336
TaaI ACNGT 2 cut(s) 94, 376
TaiI ACGT 1 cut(s) 327
TaqI TCGA 6 cut(s) 99, 219, 321, 327, 342, 399
TatI WGTACW 1 cut(s) 222
TauI GCSGC 2 cut(s) 65, 352
TfiI GAWTC 1 cut(s) 180
TscAI CASTG 1 cut(s) 513
TspDTI ATGAA 1 cut(s) 413
TspGWI ACGGA 3 cut(s) 136, 293, 489
TspRI CASTG 1 cut(s) 513
VpaK11BI GGWCC 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 231
ZraI GACGTC 1 cut(s) 325
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.