Rh5DG563700

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006472
Physical Location & Seq
Forward (+)
86800963 .. 86803186
2224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG563700.1

Sequence Viewer

Length: 561 bp
ATGGCTCCATTTCCAAAACCCCATCCGGGTCTAATCCCAATCCTCCTCCTCCTCCTCTCCACCGCCGCAAGCCCCTCACTTTCTCTCTCCTCCGACTCCTCCGCCTCGGTCTTCGAGCTCTTACCCAAATATGGGCTCCCCAGTGGGCTCCTACCCGCCTCGGTTTCCAACTACACGTTGTCCGCCGACGACGGCCGATTCGTCGTCGTTTTGGATAAGCCGTGCTATATTCAGTTCGAGTACTTGGTGTACTACGAGACCCGCATCACCGGAAAGCTGAGCTACGGCGCGATCACGGAGCTGAAGGGCATTCAGGTCCAAAGATTGTTCTTTTGGTTCGACGTCGATGAGATCAGGGTCGATTTGCCGCCGTCCGATAGTATTTACTTCACCGTTGGGATCATCAACAAGAAGCTCGATGTCGATCAGTTCCAGAGCGTTCATTCTTGCCGGGACGGGCTTTCGGGTTCGGGTTCGGGTTCTTGCCTCGGGTCGTTGAAACGGGTCATTCAGCTTCCATCTCCACTGGAAGAAGAGATAAAGATGCTACTTACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

186

Amino Acids

20.41

Weight (kDa)

5.04

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 39 - 145 4.8e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
AccII CGCG 1 cut(s) 290
AciI CCGC 7 cut(s) 63, 66, 102, 156, 183, 262, 368
AclWI GGATC 1 cut(s) 407
AcoI YGGCCR 1 cut(s) 193
AcuI CTGAAG 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 2 cut(s) 242, 251
AfiI CCNNNNNNNGG 3 cut(s) 26, 131, 132
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 1 cut(s) 499
AluBI AGCT 6 cut(s) 118, 277, 282, 301, 415, 514
AluI AGCT 6 cut(s) 118, 277, 282, 301, 415, 514
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 1 cut(s) 193
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 2 cut(s) 27, 452
AsuHPI GGTGA 2 cut(s) 259, 382
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 1 cut(s) 316
BanII GRGCYC 3 cut(s) 120, 138, 150
BbsI GAAGAC 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 120
BccI CCATC 2 cut(s) 30, 526
BceAI ACGGC 4 cut(s) 205, 208, 301, 355
BcnI CCSGG 2 cut(s) 27, 452
BcoDI GTCTC 1 cut(s) 251
BisI GCNGC 2 cut(s) 66, 368
BlpI GCTNAGC 1 cut(s) 278
BlsI GCNGC 2 cut(s) 67, 369
BmcAI AGTACT 1 cut(s) 242
Bme1390I CCNGG 2 cut(s) 27, 452
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 3 cut(s) 6, 137, 149
BmrFI CCNGG 2 cut(s) 27, 452
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 2 cut(s) 273, 534
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 103
Bpu1102I GCTNAGC 1 cut(s) 278
BpuMI CCSGG 2 cut(s) 27, 452
BsaBI GATNNNNATC 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 3 cut(s) 105, 159, 487
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 131, 132
Bse1I ACTGG 2 cut(s) 141, 531
Bse8I GATNNNNATC 1 cut(s) 423
BseDI CCNNGG 3 cut(s) 105, 159, 487
BseGI GGATG 1 cut(s) 22
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 3 cut(s) 26, 131, 132
BseMII CTCAG 2 cut(s) 269, 546
BseNI ACTGG 2 cut(s) 141, 531
BseRI GAGGAG 6 cut(s) 35, 38, 41, 44, 79, 88
BseX3I CGGCCG 1 cut(s) 193
Bsh1236I CGCG 1 cut(s) 290
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 1 cut(s) 195
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 3 cut(s) 26, 270, 451
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 3 cut(s) 26, 131, 132
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 309
BsnI GGCC 1 cut(s) 195
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 3 cut(s) 120, 138, 150
Bsp143I GATC 4 cut(s) 291, 351, 399, 424
Bsp1720I GCTNAGC 1 cut(s) 278
BspACI CCGC 7 cut(s) 63, 66, 102, 156, 183, 262, 368
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 2 cut(s) 270, 547
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 3 cut(s) 6, 137, 149
BspPI GGATC 1 cut(s) 407
BspTNI GGTCTC 1 cut(s) 251
BsrI ACTGG 2 cut(s) 141, 531
BssECI CCNNGG 3 cut(s) 105, 159, 487
BssMI GATC 4 cut(s) 291, 351, 399, 424
BssNI GRCGYC 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 1 cut(s) 528
BstACI GRCGYC 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 2 cut(s) 278, 555
BstF5I GGATG 1 cut(s) 22
BstFNI CGCG 1 cut(s) 290
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 4 cut(s) 294, 354, 402, 427
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 4 cut(s) 291, 351, 399, 424
BstMCI CGRYCG 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 25, 450
BstUI CGCG 1 cut(s) 290
BstV2I GAAGAC 1 cut(s) 103
BstZI CGGCCG 1 cut(s) 193
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 148, 524
Cac8I GCNNGC 1 cut(s) 70
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 316
Csp6I GTAC 2 cut(s) 241, 250
CviQI GTAC 2 cut(s) 241, 250
DdeI CTNAG 2 cut(s) 278, 555
DpnI GATC 4 cut(s) 293, 353, 401, 426
DpnII GATC 4 cut(s) 291, 351, 399, 424
EaeI YGGCCR 1 cut(s) 193
EagI CGGCCG 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
EciI GGCGGA 2 cut(s) 91, 172
Ecl136II GAGCTC 1 cut(s) 118
EclXI CGGCCG 1 cut(s) 193
Eco24I GRGCYC 3 cut(s) 120, 138, 150
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 316
Eco52I CGGCCG 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 118
EcoT38I GRGCYC 3 cut(s) 120, 138, 150
FaiI YATR 2 cut(s) 132, 228
FaqI GGGAC 1 cut(s) 467
FauI CCCGC 2 cut(s) 163, 269
Fnu4HI GCNGC 2 cut(s) 66, 368
FokI GGATG 1 cut(s) 9
FriOI GRGCYC 3 cut(s) 120, 138, 150
Fsp4HI GCNGC 2 cut(s) 66, 368
GlaI GCGC 1 cut(s) 289
GluI GCNGC 2 cut(s) 66, 368
HaeIII GGCC 1 cut(s) 195
HapII CCGG 3 cut(s) 26, 270, 451
HhaI GCGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 342
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HinfI GANTC 2 cut(s) 95, 198
HpaII CCGG 3 cut(s) 26, 270, 451
HphI GGTGA 2 cut(s) 259, 382
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 94, 376
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 6 cut(s) 191, 194, 206, 209, 344, 347
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 2 cut(s) 176, 342
HpyF3I CTNAG 2 cut(s) 278, 555
HpySE526I ACGT 2 cut(s) 176, 342
Hsp92I GRCGYC 1 cut(s) 342
HspAI GCGC 1 cut(s) 288
Kzo9I GATC 4 cut(s) 291, 351, 399, 424
LmnI GCTCC 4 cut(s) 10, 141, 153, 298
LpnPI CCDG 8 cut(s) 39, 154, 283, 299, 340, 446, 464, 512
LweI GCATC 2 cut(s) 273, 534
MaeII ACGT 2 cut(s) 176, 342
MalI GATC 4 cut(s) 293, 353, 401, 426
MboI GATC 4 cut(s) 291, 351, 399, 424
MboII GAAGA 3 cut(s) 103, 542, 545
MhlI GDGCHC 3 cut(s) 120, 138, 150
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 117, 192
MspI CCGG 3 cut(s) 26, 270, 451
MspR9I CCNGG 2 cut(s) 27, 452
Mva1269I GAATGC 1 cut(s) 309
MvnI CGCG 1 cut(s) 290
NciI CCSGG 2 cut(s) 27, 452
NdeII GATC 4 cut(s) 291, 351, 399, 424
NlaIV GGNNCC 3 cut(s) 6, 137, 149
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 309
PfeI GAWTC 1 cut(s) 198
PkrI GCNGC 2 cut(s) 67, 369
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp124BI GAGCTC 1 cut(s) 120
PspN4I GGNNCC 3 cut(s) 6, 137, 149
PspPI GGNCC 1 cut(s) 316
RsaI GTAC 2 cut(s) 242, 251
RsaNI GTAC 2 cut(s) 241, 250
SacI GAGCTC 1 cut(s) 120
SatI GCNGC 2 cut(s) 66, 368
Sau3AI GATC 4 cut(s) 291, 351, 399, 424
Sau96I GGNCC 1 cut(s) 316
ScaI AGTACT 1 cut(s) 242
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 27, 452
SduI GDGCHC 3 cut(s) 120, 138, 150
SetI ASST 9 cut(s) 120, 179, 279, 284, 303, 318, 345, 417, 516
SfaNI GCATC 2 cut(s) 273, 534
SinI GGWCC 1 cut(s) 316
SsiI CCGC 7 cut(s) 63, 66, 102, 156, 183, 262, 368
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 25, 450
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 2 cut(s) 179, 345
TaqI TCGA 7 cut(s) 114, 237, 339, 345, 360, 417, 423
TaqII GACCGA 1 cut(s) 97
TatI WGTACW 2 cut(s) 240, 249
TauI GCSGC 2 cut(s) 68, 370
TfiI GAWTC 1 cut(s) 198
TscAI CASTG 2 cut(s) 148, 531
TspDTI ATGAA 1 cut(s) 431
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 2 cut(s) 148, 531
VpaK11BI GGWCC 1 cut(s) 316
ZraI GACGTC 1 cut(s) 343
ZrmI AGTACT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.