Rh5DG563800

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006472
Physical Location & Seq
Forward (+)
86825822 .. 86838149
12328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG563800.1

Sequence Viewer

Length: 597 bp
ATGCCAATCTTCCACACGTCCGCTGCTACCATTGACCATCCGCCGTCGCTATCGCCGTCTGCAATCCTGACAGTTTTCTCACAACAGTCGTTGCCACCACCACCAATTTGGATGCTAACCTCTGCCGTCGTCTGCCCGAGAATCAGGCCTGCAACTCCATCCTCAAAATCTGCAGATCTGCCTCCGTCGAAAGCCAACAAGGAAGAGAAAGAGAGCCTCGCCACCAAGCCTTGTGCCGACAGCCTCCTCGCCACAAAAGCCCGAGTCCCTCCATTCGACTACTTGGTGTACTACGAGACCCGCATCACCGGAAAGCTAAGCTACGGCGCGATCACGGAGCTGAAGGGCATTCAGGTCCAAAGATTGTTCTTTTGGTTCGACGTCGATGAGATCAGGGTCGATTTGCCGCCGTCCGATAGTATTTACTTCACCGTTGGGATCATCAACAAGAAGCTCGATGTCGATCAGTTCCAGAGCGTTCATTCTTGCCGGGACGGGCTTTCGGGTTCGGGTTCGGGTTCTTGCCTCGGGTCGTTGAAACGGGTCATTCAGCTTCCATCTCCACTGGAAGAAGAGATAAAGATGCCTGCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

198

Amino Acids

21.71

Weight (kDa)

6.82

Isoelectric Point (pI)

61.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 93 - 158 1.1e-17 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 384
AccII CGCG 1 cut(s) 329
AciI CCGC 4 cut(s) 21, 41, 301, 407
AclWI GGATC 1 cut(s) 446
AcuI CTGAAG 1 cut(s) 362
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 1 cut(s) 538
AjiI CACGTC 1 cut(s) 18
AluBI AGCT 5 cut(s) 316, 321, 340, 454, 553
AluI AGCT 5 cut(s) 316, 321, 340, 454, 553
Alw26I GTCTC 1 cut(s) 290
AlwI GGATC 1 cut(s) 446
Ama87I CYCGRG 3 cut(s) 136, 261, 527
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 1 cut(s) 355
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 2 cut(s) 298, 421
AvaI CYCGRG 3 cut(s) 136, 261, 527
AvaII GGWCC 1 cut(s) 355
BbvI GCAGC 1 cut(s) 10
BccI CCATC 3 cut(s) 45, 166, 565
BceAI ACGGC 5 cut(s) 28, 40, 110, 340, 394
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 171
BglII AGATCT 1 cut(s) 175
BisI GCNGC 2 cut(s) 24, 407
BlpI GCTNAGC 1 cut(s) 317
BlsI GCNGC 2 cut(s) 25, 408
Bme1390I CCNGG 1 cut(s) 491
Bme18I GGWCC 1 cut(s) 355
BmeT110I CYCGRG 3 cut(s) 136, 261, 527
BmgBI CACGTC 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 491
BmsI GCATC 3 cut(s) 102, 312, 573
Bpu1102I GCTNAGC 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 491
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 1 cut(s) 526
BsaWI WCCGGW 1 cut(s) 308
Bse1I ACTGG 1 cut(s) 570
Bse8I GATNNNNATC 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 526
BseGI GGATG 3 cut(s) 37, 117, 158
BseJI GATNNNNATC 1 cut(s) 462
BseNI ACTGG 1 cut(s) 570
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 1 cut(s) 148
BsiHKCI CYCGRG 3 cut(s) 136, 261, 527
BsiSI CCGG 2 cut(s) 309, 490
BslFI GGGAC 2 cut(s) 251, 506
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 2 cut(s) 251, 506
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 1 cut(s) 148
Bso31I GGTCTC 1 cut(s) 290
BsoBI CYCGRG 3 cut(s) 136, 261, 527
Bsp143I GATC 5 cut(s) 175, 330, 390, 438, 463
Bsp1720I GCTNAGC 1 cut(s) 317
BspACI CCGC 4 cut(s) 21, 41, 301, 407
BspANI GGCC 1 cut(s) 148
BspFNI CGCG 1 cut(s) 329
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 290
BsrI ACTGG 1 cut(s) 570
BssECI CCNNGG 1 cut(s) 526
BssMI GATC 5 cut(s) 175, 330, 390, 438, 463
BssNI GRCGYC 1 cut(s) 381
Bst4CI ACNGT 3 cut(s) 73, 87, 433
Bst6I CTCTTC 2 cut(s) 198, 567
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 2 cut(s) 150, 588
BstDEI CTNAG 1 cut(s) 317
BstF5I GGATG 3 cut(s) 37, 117, 158
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 5 cut(s) 178, 333, 393, 441, 466
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 5 cut(s) 175, 330, 390, 438, 463
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 489
BstSFI CTRYAG 1 cut(s) 171
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 175
BstXI CCANNNNNNTGG 1 cut(s) 108
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 1 cut(s) 148
BtrI CACGTC 1 cut(s) 18
BtsCI GGATG 3 cut(s) 37, 117, 158
BtsIMutI CAGTG 1 cut(s) 563
Cac8I GCNNGC 2 cut(s) 150, 588
CfoI GCGC 1 cut(s) 329
Cfr13I GGNCC 1 cut(s) 355
Csp6I GTAC 1 cut(s) 289
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 317
DpnI GATC 5 cut(s) 177, 332, 392, 440, 465
DpnII GATC 5 cut(s) 175, 330, 390, 438, 463
Eam1104I CTCTTC 2 cut(s) 198, 567
EarI CTCTTC 2 cut(s) 198, 567
EciI GGCGGA 1 cut(s) 30
Eco147I AGGCCT 1 cut(s) 148
Eco31I GGTCTC 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 362
Eco88I CYCGRG 3 cut(s) 136, 261, 527
FaqI GGGAC 2 cut(s) 251, 506
FauI CCCGC 1 cut(s) 308
Fnu4HI GCNGC 2 cut(s) 24, 407
FokI GGATG 3 cut(s) 24, 124, 145
Fsp4HI GCNGC 2 cut(s) 24, 407
GlaI GCGC 1 cut(s) 328
GluI GCNGC 2 cut(s) 24, 407
HaeIII GGCC 1 cut(s) 148
HapII CCGG 2 cut(s) 309, 490
HhaI GCGC 1 cut(s) 329
Hin1I GRCGYC 1 cut(s) 381
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HinfI GANTC 2 cut(s) 141, 264
HpaII CCGG 2 cut(s) 309, 490
HphI GGTGA 2 cut(s) 298, 421
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 1 cut(s) 415
Hpy188III TCNNGA 2 cut(s) 67, 472
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 5 cut(s) 49, 131, 190, 383, 386
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 3 cut(s) 73, 87, 433
HpyCH4IV ACGT 2 cut(s) 17, 381
HpyCH4V TGCA 3 cut(s) 62, 152, 173
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 1 cut(s) 317
HpySE526I ACGT 2 cut(s) 17, 381
Hsp92I GRCGYC 1 cut(s) 381
HspAI GCGC 1 cut(s) 327
Kzo9I GATC 5 cut(s) 175, 330, 390, 438, 463
LmnI GCTCC 1 cut(s) 337
LpnPI CCDG 9 cut(s) 80, 130, 162, 322, 338, 379, 485, 503, 551
Lsp1109I GCAGC 1 cut(s) 10
LweI GCATC 3 cut(s) 102, 312, 573
MaeII ACGT 2 cut(s) 17, 381
MalI GATC 5 cut(s) 177, 332, 392, 440, 465
MboI GATC 5 cut(s) 175, 330, 390, 438, 463
MboII GAAGA 3 cut(s) 215, 581, 584
MflI RGATCY 1 cut(s) 175
MluCI AATT 1 cut(s) 105
MlyI GAGTC 1 cut(s) 273
MnlI CCTC 8 cut(s) 130, 172, 192, 227, 254, 257, 279, 536
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 2 cut(s) 309, 490
MspR9I CCNGG 1 cut(s) 491
Mva1269I GAATGC 1 cut(s) 348
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 1 cut(s) 257
NciI CCSGG 1 cut(s) 491
NdeII GATC 5 cut(s) 175, 330, 390, 438, 463
PceI AGGCCT 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 141
PkrI GCNGC 2 cut(s) 25, 408
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PspPI GGNCC 1 cut(s) 355
PstI CTGCAG 1 cut(s) 175
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SatI GCNGC 2 cut(s) 24, 407
Sau3AI GATC 5 cut(s) 175, 330, 390, 438, 463
Sau96I GGNCC 1 cut(s) 355
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 1 cut(s) 491
SetI ASST 9 cut(s) 20, 122, 318, 323, 342, 357, 384, 456, 555
SfaNI GCATC 3 cut(s) 102, 312, 573
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 1 cut(s) 355
Sse9I AATT 1 cut(s) 105
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 4 cut(s) 21, 41, 301, 407
StuI AGGCCT 1 cut(s) 148
StyD4I CCNGG 1 cut(s) 489
TaaI ACNGT 3 cut(s) 73, 87, 433
TaiI ACGT 2 cut(s) 20, 384
TaqI TCGA 7 cut(s) 188, 276, 378, 384, 399, 456, 462
TasI AATT 1 cut(s) 105
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 1 cut(s) 141
TscAI CASTG 1 cut(s) 570
TseI GCWGC 1 cut(s) 23
TspDTI ATGAA 1 cut(s) 470
TspGWI ACGGA 2 cut(s) 174, 350
TspRI CASTG 1 cut(s) 570
VpaK11BI GGWCC 1 cut(s) 355
XcmI CCANNNNNNNNNTGG 1 cut(s) 105
ZraI GACGTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.