Rroxscaffold_1G00001980

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006472
Physical Location & Seq
Reverse (-)
2412198 .. 2415786
3589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00001980.1

Sequence Viewer

Length: 567 bp
ATGGCTCCATTTCCAAAACCCCATCCGGGTCTAATCCCAATCCTCCTCCTCCTCCTCTCCACCGCCGCAAGCCCCTCTCTTTCTATCTCCTCCGACTCCTCCGCCTCGGTCTTCGAGCTATTACCCAAATTCGGGCTCCCCAGTGGGCTCCTACCCGCCTCGGTTTCCAACTACACGTTGTCCGCCGACGACGGCCGATTCGTCGTCGTTTTGGAGAAGCCGTGCTATATTCAGTTCGAGTACTTGGTGTACTACGAGACCCGCATCACCGGAAAACTGAGCTACGGCGCGATCACGGAGCTGAAGGGCATTCAGGTCCAAAGATTGTTCTTTTGGTTCGACGTCGATGAGATCAGGGTCGATTTGCCGCCGTCCGATAGTATTTACTTCACCGTTGGGATCATCAACAAGAAGCTCGATGTCGATCAGTTCCAGAGCGTTCATTCTTGCCGCGACGGGCTTTCGGGTTCGGGTTCGGGTTCGGGTTCTTGCCTCGGGTCCTTGAAACGGGTCATTCAGCTTCCATCTCCACTGGAAGAAGAGATAAAGATGCTACTTACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

188

Amino Acids

20.55

Weight (kDa)

5.05

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 39 - 145 1.1e-32 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
AccII CGCG 2 cut(s) 290, 453
AciI CCGC 8 cut(s) 63, 66, 102, 156, 183, 262, 368, 451
AclWI GGATC 1 cut(s) 407
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 2 cut(s) 242, 251
AfiI CCNNNNNNNGG 4 cut(s) 26, 131, 132, 507
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 1 cut(s) 505
AluBI AGCT 5 cut(s) 118, 282, 301, 415, 520
AluI AGCT 5 cut(s) 118, 282, 301, 415, 520
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 407
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 1 cut(s) 193
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 2 cut(s) 316, 498
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 259, 382
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 2 cut(s) 316, 498
BanII GRGCYC 2 cut(s) 138, 150
BbsI GAAGAC 1 cut(s) 103
BccI CCATC 2 cut(s) 30, 532
BceAI ACGGC 4 cut(s) 205, 208, 301, 355
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 251
BisI GCNGC 3 cut(s) 66, 368, 451
BlsI GCNGC 3 cut(s) 67, 369, 452
BmcAI AGTACT 1 cut(s) 242
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 2 cut(s) 316, 498
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 2 cut(s) 316, 498
BmiI GGNNCC 4 cut(s) 6, 137, 149, 499
BmrFI CCNGG 1 cut(s) 27
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 2 cut(s) 273, 540
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 103
BpuMI CCSGG 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 3 cut(s) 105, 159, 493
BsaWI WCCGGW 1 cut(s) 269
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 4 cut(s) 26, 131, 132, 507
Bse1I ACTGG 2 cut(s) 141, 537
Bse8I GATNNNNATC 1 cut(s) 423
BseDI CCNNGG 3 cut(s) 105, 159, 493
BseGI GGATG 1 cut(s) 22
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 4 cut(s) 26, 131, 132, 507
BseMII CTCAG 2 cut(s) 269, 552
BseNI ACTGG 2 cut(s) 141, 537
BseRI GAGGAG 6 cut(s) 35, 38, 41, 44, 79, 88
BseX3I CGGCCG 1 cut(s) 193
Bsh1236I CGCG 2 cut(s) 290, 453
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 1 cut(s) 195
BsiEI CGRYCG 1 cut(s) 196
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 2 cut(s) 26, 270
BslI CCNNNNNNNGG 4 cut(s) 26, 131, 132, 507
BsmAI GTCTC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 309
BsnI GGCC 1 cut(s) 195
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 494
Bsp1286I GDGCHC 2 cut(s) 138, 150
Bsp143I GATC 4 cut(s) 291, 351, 399, 424
BspACI CCGC 8 cut(s) 63, 66, 102, 156, 183, 262, 368, 451
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 2 cut(s) 270, 553
BspFNI CGCG 2 cut(s) 290, 453
BspLI GGNNCC 4 cut(s) 6, 137, 149, 499
BspPI GGATC 1 cut(s) 407
BspTNI GGTCTC 1 cut(s) 251
BsrI ACTGG 2 cut(s) 141, 537
BssECI CCNNGG 3 cut(s) 105, 159, 493
BssMI GATC 4 cut(s) 291, 351, 399, 424
BssNI GRCGYC 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 1 cut(s) 534
BstACI GRCGYC 1 cut(s) 342
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 2 cut(s) 278, 561
BstF5I GGATG 1 cut(s) 22
BstFNI CGCG 2 cut(s) 290, 453
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 4 cut(s) 294, 354, 402, 427
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 4 cut(s) 291, 351, 399, 424
BstMCI CGRYCG 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 25
BstUI CGCG 2 cut(s) 290, 453
BstV2I GAAGAC 1 cut(s) 103
BstZI CGGCCG 1 cut(s) 193
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 148, 530
Cac8I GCNNGC 1 cut(s) 70
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 2 cut(s) 316, 498
Csp6I GTAC 2 cut(s) 241, 250
CviQI GTAC 2 cut(s) 241, 250
DdeI CTNAG 2 cut(s) 278, 561
DpnI GATC 4 cut(s) 293, 353, 401, 426
DpnII GATC 4 cut(s) 291, 351, 399, 424
EaeI YGGCCR 1 cut(s) 193
EagI CGGCCG 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
EciI GGCGGA 2 cut(s) 91, 172
EclXI CGGCCG 1 cut(s) 193
Eco24I GRGCYC 2 cut(s) 138, 150
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 2 cut(s) 316, 498
Eco52I CGGCCG 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 498
EcoT38I GRGCYC 2 cut(s) 138, 150
FaiI YATR 1 cut(s) 228
FauI CCCGC 2 cut(s) 163, 269
Fnu4HI GCNGC 3 cut(s) 66, 368, 451
FokI GGATG 1 cut(s) 9
FriOI GRGCYC 2 cut(s) 138, 150
Fsp4HI GCNGC 3 cut(s) 66, 368, 451
GlaI GCGC 1 cut(s) 289
GluI GCNGC 3 cut(s) 66, 368, 451
HaeIII GGCC 1 cut(s) 195
HapII CCGG 2 cut(s) 26, 270
HhaI GCGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 342
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HinfI GANTC 2 cut(s) 95, 198
HpaII CCGG 2 cut(s) 26, 270
HphI GGTGA 2 cut(s) 259, 382
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 94, 376
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 7 cut(s) 191, 194, 206, 209, 344, 347, 458
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 2 cut(s) 176, 342
HpyF3I CTNAG 2 cut(s) 278, 561
HpySE526I ACGT 2 cut(s) 176, 342
Hsp92I GRCGYC 1 cut(s) 342
HspAI GCGC 1 cut(s) 288
Kzo9I GATC 4 cut(s) 291, 351, 399, 424
LmnI GCTCC 4 cut(s) 10, 141, 153, 298
LpnPI CCDG 7 cut(s) 39, 154, 283, 299, 340, 446, 518
LweI GCATC 2 cut(s) 273, 540
MaeII ACGT 2 cut(s) 176, 342
MalI GATC 4 cut(s) 293, 353, 401, 426
MboI GATC 4 cut(s) 291, 351, 399, 424
MboII GAAGA 3 cut(s) 103, 548, 551
MhlI GDGCHC 2 cut(s) 138, 150
MluCI AATT 1 cut(s) 128
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 117, 192
MspI CCGG 2 cut(s) 26, 270
MspR9I CCNGG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 309
MvnI CGCG 2 cut(s) 290, 453
NciI CCSGG 1 cut(s) 27
NdeII GATC 4 cut(s) 291, 351, 399, 424
NlaIV GGNNCC 4 cut(s) 6, 137, 149, 499
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 309
PfeI GAWTC 1 cut(s) 198
PkrI GCNGC 3 cut(s) 67, 369, 452
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
PpuMI RGGWCCY 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 498
PspN4I GGNNCC 4 cut(s) 6, 137, 149, 499
PspPI GGNCC 2 cut(s) 316, 498
PspPPI RGGWCCY 1 cut(s) 498
RsaI GTAC 2 cut(s) 242, 251
RsaNI GTAC 2 cut(s) 241, 250
SatI GCNGC 3 cut(s) 66, 368, 451
Sau3AI GATC 4 cut(s) 291, 351, 399, 424
Sau96I GGNCC 2 cut(s) 316, 498
ScaI AGTACT 1 cut(s) 242
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 2 cut(s) 138, 150
SetI ASST 8 cut(s) 120, 179, 284, 303, 318, 345, 417, 522
SfaNI GCATC 2 cut(s) 273, 540
SinI GGWCC 2 cut(s) 316, 498
Sse9I AATT 1 cut(s) 128
SsiI CCGC 8 cut(s) 63, 66, 102, 156, 183, 262, 368, 451
StyD4I CCNGG 1 cut(s) 25
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 2 cut(s) 179, 345
TaqI TCGA 7 cut(s) 114, 237, 339, 345, 360, 417, 423
TaqII GACCGA 1 cut(s) 97
TasI AATT 1 cut(s) 128
TatI WGTACW 2 cut(s) 240, 249
TauI GCSGC 3 cut(s) 68, 370, 453
TfiI GAWTC 1 cut(s) 198
TscAI CASTG 2 cut(s) 148, 537
TspDTI ATGAA 1 cut(s) 431
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 2 cut(s) 148, 537
VpaK11BI GGWCC 2 cut(s) 316, 498
XapI RAATTY 1 cut(s) 128
ZraI GACGTC 1 cut(s) 343
ZrmI AGTACT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.