Rw5G049200

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006472
Physical Location & Seq
Forward (+)
85279090 .. 85281946
2857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049200.1

Sequence Viewer

Length: 573 bp
ATGGCTCCATTTCCAAAACCCCATCCGGGTCTAATCCCAATCCTCCTCCTCCTCCTCCTCCTCTCCACCGCCGCAAGCCCCTCTCTTTCTCTCTCCTCCGACTCCTCCGCCTCGGTCTTCGAGCTCTTACCCAAATATGGGCTGCCCAGTGGGCTCCTACCCGCCTCGGTTTCCAACTACACGTTGTCCGCCGACGACGGCCGATTCGTCGTCGTTTTGGAGAAGCCGTGCTATATTCAGTTCGAGTACTTGGTGTACTACGAGACCCGCATCACCGGCAAGCTGAGCTACGGCGCGATCACGGAGCTGAAGGGCATTCAGGTCCAAAGATTGTTCTTTTGGTTCGACGTCGATGAGATCAGGGTCGATTTGCCGCCGTCCGATAGTATTTACTTCACGGTTGGGATCATCAACAAGAAGCTCGATGTCGATCAGTTCCAGAGCGTTCATTCTTGCCGCGACGGGCTTTCGGGTTCGGGTTCGGGTTCGGGTTCTTGCCTCGGGTCGTTGAAACGGGTCATTCAGCTTCCATCTCCACTGGAAGAAGAGATAAAGATGCTACTTACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

190

Amino Acids

20.79

Weight (kDa)

5.05

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 41 - 147 4.8e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
AccII CGCG 2 cut(s) 296, 459
AciI CCGC 8 cut(s) 69, 72, 108, 162, 189, 268, 374, 457
AclWI GGATC 1 cut(s) 413
AcoI YGGCCR 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 348
AfaI GTAC 2 cut(s) 248, 257
AfiI CCNNNNNNNGG 3 cut(s) 26, 137, 138
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 1 cut(s) 511
AluBI AGCT 6 cut(s) 124, 283, 288, 307, 421, 526
AluI AGCT 6 cut(s) 124, 283, 288, 307, 421, 526
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 1 cut(s) 413
Ama87I CYCGRG 1 cut(s) 500
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 1 cut(s) 142
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 500
AvaII GGWCC 1 cut(s) 322
BanII GRGCYC 2 cut(s) 126, 156
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 129
BccI CCATC 2 cut(s) 30, 538
BceAI ACGGC 4 cut(s) 211, 214, 307, 361
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 257
BglI GCCNNNNNGGC 1 cut(s) 151
BisI GCNGC 4 cut(s) 72, 143, 374, 457
BlpI GCTNAGC 1 cut(s) 284
BlsI GCNGC 4 cut(s) 73, 144, 375, 458
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 2 cut(s) 6, 155
BmrFI CCNGG 1 cut(s) 27
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 2 cut(s) 279, 546
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 109
Bpu1102I GCTNAGC 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 111, 165, 499
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 137, 138
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 147, 543
Bse8I GATNNNNATC 1 cut(s) 429
BseDI CCNNGG 3 cut(s) 111, 165, 499
BseGI GGATG 1 cut(s) 22
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 26, 137, 138
BseMII CTCAG 2 cut(s) 275, 558
BseNI ACTGG 2 cut(s) 147, 543
BseRI GAGGAG 8 cut(s) 35, 38, 41, 44, 47, 50, 85, 94
BseX3I CGGCCG 1 cut(s) 199
BseXI GCAGC 1 cut(s) 129
Bsh1236I CGCG 2 cut(s) 296, 459
Bsh1285I CGRYCG 1 cut(s) 202
BshFI GGCC 1 cut(s) 201
BsiEI CGRYCG 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 500
BsiSI CCGG 2 cut(s) 26, 276
BslI CCNNNNNNNGG 3 cut(s) 26, 137, 138
BsmAI GTCTC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 1 cut(s) 201
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 500
Bsp1286I GDGCHC 2 cut(s) 126, 156
Bsp143I GATC 4 cut(s) 297, 357, 405, 430
Bsp1720I GCTNAGC 1 cut(s) 284
BspACI CCGC 8 cut(s) 69, 72, 108, 162, 189, 268, 374, 457
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 2 cut(s) 276, 559
BspFNI CGCG 2 cut(s) 296, 459
BspLI GGNNCC 2 cut(s) 6, 155
BspPI GGATC 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 257
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 2 cut(s) 147, 543
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 111, 165, 499
BssMI GATC 4 cut(s) 297, 357, 405, 430
BssNI GRCGYC 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 1 cut(s) 540
BstACI GRCGYC 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 76, 281
BstDEI CTNAG 2 cut(s) 284, 567
BstF5I GGATG 1 cut(s) 22
BstFNI CGCG 2 cut(s) 296, 459
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 4 cut(s) 300, 360, 408, 433
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 4 cut(s) 297, 357, 405, 430
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 3 cut(s) 151, 276, 285
BstSCI CCNGG 1 cut(s) 25
BstUI CGCG 2 cut(s) 296, 459
BstV1I GCAGC 1 cut(s) 129
BstV2I GAAGAC 1 cut(s) 109
BstZI CGGCCG 1 cut(s) 199
BsuRI GGCC 1 cut(s) 201
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 2 cut(s) 154, 536
Cac8I GCNNGC 2 cut(s) 76, 281
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 247, 256
CviQI GTAC 2 cut(s) 247, 256
DdeI CTNAG 2 cut(s) 284, 567
DpnI GATC 4 cut(s) 299, 359, 407, 432
DpnII GATC 4 cut(s) 297, 357, 405, 430
EaeI YGGCCR 1 cut(s) 199
EagI CGGCCG 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
EciI GGCGGA 2 cut(s) 97, 178
Ecl136II GAGCTC 1 cut(s) 124
EclXI CGGCCG 1 cut(s) 199
Eco24I GRGCYC 2 cut(s) 126, 156
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 322
Eco52I CGGCCG 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 500
EcoICRI GAGCTC 1 cut(s) 124
EcoT38I GRGCYC 2 cut(s) 126, 156
FaiI YATR 2 cut(s) 138, 234
FauI CCCGC 2 cut(s) 169, 275
Fnu4HI GCNGC 4 cut(s) 72, 143, 374, 457
FokI GGATG 1 cut(s) 9
FriOI GRGCYC 2 cut(s) 126, 156
Fsp4HI GCNGC 4 cut(s) 72, 143, 374, 457
GlaI GCGC 1 cut(s) 295
GluI GCNGC 4 cut(s) 72, 143, 374, 457
HaeIII GGCC 1 cut(s) 201
HapII CCGG 2 cut(s) 26, 276
HhaI GCGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 348
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HinfI GANTC 2 cut(s) 101, 204
HpaII CCGG 2 cut(s) 26, 276
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 2 cut(s) 100, 382
Hpy188III TCNNGA 1 cut(s) 439
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 7 cut(s) 197, 200, 212, 215, 350, 353, 464
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 2 cut(s) 182, 348
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 276, 285
HpyF3I CTNAG 2 cut(s) 284, 567
HpySE526I ACGT 2 cut(s) 182, 348
Hsp92I GRCGYC 1 cut(s) 348
HspAI GCGC 1 cut(s) 294
Kzo9I GATC 4 cut(s) 297, 357, 405, 430
LmnI GCTCC 3 cut(s) 10, 159, 304
LpnPI CCDG 7 cut(s) 39, 160, 289, 305, 346, 452, 524
Lsp1109I GCAGC 1 cut(s) 129
LweI GCATC 2 cut(s) 279, 546
MaeII ACGT 2 cut(s) 182, 348
MalI GATC 4 cut(s) 299, 359, 407, 432
MboI GATC 4 cut(s) 297, 357, 405, 430
MboII GAAGA 3 cut(s) 109, 554, 557
MhlI GDGCHC 2 cut(s) 126, 156
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 2 cut(s) 123, 198
MspI CCGG 2 cut(s) 26, 276
MspR9I CCNGG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 315
MvnI CGCG 2 cut(s) 296, 459
MwoI GCNNNNNNNGC 3 cut(s) 151, 276, 285
NciI CCSGG 1 cut(s) 27
NdeII GATC 4 cut(s) 297, 357, 405, 430
NlaIV GGNNCC 2 cut(s) 6, 155
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 204
PkrI GCNGC 4 cut(s) 73, 144, 375, 458
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 126
PspN4I GGNNCC 2 cut(s) 6, 155
PspPI GGNCC 1 cut(s) 322
RsaI GTAC 2 cut(s) 248, 257
RsaNI GTAC 2 cut(s) 247, 256
SacI GAGCTC 1 cut(s) 126
SatI GCNGC 4 cut(s) 72, 143, 374, 457
Sau3AI GATC 4 cut(s) 297, 357, 405, 430
Sau96I GGNCC 1 cut(s) 322
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 2 cut(s) 126, 156
SetI ASST 9 cut(s) 126, 185, 285, 290, 309, 324, 351, 423, 528
SfaNI GCATC 2 cut(s) 279, 546
SinI GGWCC 1 cut(s) 322
SsiI CCGC 8 cut(s) 69, 72, 108, 162, 189, 268, 374, 457
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 25
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 2 cut(s) 185, 351
TaqI TCGA 7 cut(s) 120, 243, 345, 351, 366, 423, 429
TaqII GACCGA 1 cut(s) 103
TatI WGTACW 2 cut(s) 246, 255
TauI GCSGC 3 cut(s) 74, 376, 459
TfiI GAWTC 1 cut(s) 204
TscAI CASTG 2 cut(s) 154, 543
TseI GCWGC 1 cut(s) 142
TspDTI ATGAA 1 cut(s) 437
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 2 cut(s) 154, 543
VpaK11BI GGWCC 1 cut(s) 322
ZraI GACGTC 1 cut(s) 349
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.