MD03G1007800.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006472
Physical Location & Seq
Reverse (-)
617965 .. 620909
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1007800.v1.1.491

Sequence Viewer

Length: 540 bp
ATGTCACCGACCCCGAAACCCCAACTGGGTCTGATCCCGATCCTCCTCCTCTCTCTCTCCGTCATCCCAAGTCTCTCTCTTTCTCTCTCTGACTCCCCTCCCACAGTGTTTGACATTTTACCCAAATTCGGCCTTCCCCGCGGCCTCTTACCGGCATCGGTCTCCAACTACACGCTCTCCGACGACGGACGATTCGTCGTCGTTTTGCCCAAGACCTGCTACCTGCAGTTCGATTACTTGGTCTACTACGAAAAGACCATAACGGGCAAGCTCACGTACGGGGCGATCACCGACTTGAAGGGCATTCAGGTCCAGAGGTTCTTGTTCTGGCTCGGCGTCGGCGAGATCAGGGTCGATTTGCCGCCGTCCGATAACATTTACTTCACTGTGGGGATCATCAATAAGAAGCTGGATATCGGTCAGTTCCAAAATGTCCGGCCTTGCCGTGACGGGTTATCGGGTTCTTGCGTCGGGTCGTTCAAACGGGGCATTCAGCTTCCATCTCCAGTGGAAGAAATTGAGATGCTAATAACCGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

180

Amino Acids

19.75

Weight (kDa)

6.29

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 38 - 143 1.2e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 224, 231
AccI GTMKAC 1 cut(s) 243
AccII CGCG 1 cut(s) 141
AciI CCGC 3 cut(s) 139, 141, 362
AclWI GGATC 3 cut(s) 28, 34, 401
AcsI RAATTY 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 3 cut(s) 26, 128, 151
AgsI TTSAA 2 cut(s) 298, 481
AhdI GACNNNNNGTC 1 cut(s) 194
AluBI AGCT 3 cut(s) 271, 409, 496
AluI AGCT 3 cut(s) 271, 409, 496
Alw26I GTCTC 2 cut(s) 77, 166
AlwI GGATC 3 cut(s) 28, 34, 401
AoxI GGCC 3 cut(s) 130, 142, 437
ApoI RAATTY 1 cut(s) 125
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AspS9I GGNCC 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 280
AvaII GGWCC 1 cut(s) 310
BccI CCATC 1 cut(s) 508
BceAI ACGGC 2 cut(s) 349, 429
BcoDI GTCTC 2 cut(s) 77, 166
BfmI CTRYAG 1 cut(s) 224
BfuAI ACCTGC 2 cut(s) 224, 231
BisI GCNGC 2 cut(s) 142, 362
BlsI GCNGC 2 cut(s) 143, 363
Bme18I GGWCC 1 cut(s) 310
BmeRI GACNNNNNGTC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 310
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 2 cut(s) 164, 513
BmuI ACTGGG 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 489
BsaAI YACGTR 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 38
BsaHI GRCGYC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 128, 151
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 2 cut(s) 30, 506
Bse8I GATNNNNATC 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 1 cut(s) 63
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 3 cut(s) 26, 128, 151
BseNI ACTGG 2 cut(s) 30, 506
BseRI GAGGAG 2 cut(s) 35, 38
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 132, 144, 439
BsiSI CCGG 2 cut(s) 152, 436
BsiWI CGTACG 1 cut(s) 276
BslI CCNNNNNNNGG 3 cut(s) 26, 128, 151
BsmAI GTCTC 2 cut(s) 77, 166
BsmI GAATGC 2 cut(s) 303, 489
BsnI GGCC 3 cut(s) 132, 144, 439
Bso31I GGTCTC 1 cut(s) 166
Bsp143I GATC 5 cut(s) 33, 39, 285, 345, 393
BspACI CCGC 3 cut(s) 139, 141, 362
BspANI GGCC 3 cut(s) 132, 144, 439
BspFNI CGCG 1 cut(s) 141
BspMAI CTGCAG 1 cut(s) 228
BspMI ACCTGC 2 cut(s) 224, 231
BspPI GGATC 3 cut(s) 28, 34, 401
BspTNI GGTCTC 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 151
BsrI ACTGG 2 cut(s) 30, 506
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 5 cut(s) 33, 39, 285, 345, 393
BssNI GRCGYC 1 cut(s) 336
Bst4CI ACNGT 2 cut(s) 106, 388
BstACI GRCGYC 1 cut(s) 336
BstBAI YACGTR 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 269
BstDSI CCRYGG 1 cut(s) 139
BstF5I GGATG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 141
BstKTI GATC 5 cut(s) 36, 42, 288, 348, 396
BstMAI GTCTC 2 cut(s) 77, 166
BstMBI GATC 5 cut(s) 33, 39, 285, 345, 393
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 224
BstUI CGCG 1 cut(s) 141
BsuRI GGCC 3 cut(s) 132, 144, 439
BtgI CCRYGG 1 cut(s) 139
BtsCI GGATG 1 cut(s) 63
BtsIMutI CAGTG 3 cut(s) 111, 384, 513
BveI ACCTGC 2 cut(s) 224, 231
Cac8I GCNNGC 1 cut(s) 269
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 310
Cfr42I CCGCGG 1 cut(s) 142
CseI GACGC 2 cut(s) 325, 457
Csp6I GTAC 1 cut(s) 277
CspCI CAANNNNNGTGG 2 cut(s) 91, 126
CviJI RGCY 7 cut(s) 132, 144, 271, 331, 409, 439, 496
CviKI_1 RGCY 7 cut(s) 132, 144, 271, 331, 409, 439, 496
CviQI GTAC 1 cut(s) 277
DpnI GATC 5 cut(s) 35, 41, 287, 347, 395
DpnII GATC 5 cut(s) 33, 39, 285, 345, 393
DriI GACNNNNNGTC 1 cut(s) 194
Eam1105I GACNNNNNGTC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 166
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 310
EcoRV GATATC 1 cut(s) 415
FaiI YATR 1 cut(s) 260
FauI CCCGC 1 cut(s) 146
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 142, 362
FokI GGATG 1 cut(s) 50
Fsp4HI GCNGC 2 cut(s) 142, 362
GluI GCNGC 2 cut(s) 142, 362
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 3 cut(s) 132, 144, 439
HapII CCGG 2 cut(s) 152, 436
HgaI GACGC 2 cut(s) 325, 457
Hin1I GRCGYC 1 cut(s) 336
HinfI GANTC 2 cut(s) 92, 192
HpaII CCGG 2 cut(s) 152, 436
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 4 cut(s) 33, 91, 181, 370
Hpy188III TCNNGA 2 cut(s) 37, 313
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 6 cut(s) 185, 188, 200, 203, 341, 473
HpyAV CCTTC 2 cut(s) 143, 292
HpyCH4III ACNGT 2 cut(s) 106, 388
HpyCH4IV ACGT 1 cut(s) 275
HpyCH4V TGCA 1 cut(s) 226
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 275
Hsp92I GRCGYC 1 cut(s) 336
KspI CCGCGG 1 cut(s) 142
Kzo9I GATC 5 cut(s) 33, 39, 285, 345, 393
LweI GCATC 2 cut(s) 164, 513
MaeII ACGT 1 cut(s) 275
MaeIII GTNAC 2 cut(s) 3, 446
MalI GATC 5 cut(s) 35, 41, 287, 347, 395
MboI GATC 5 cut(s) 33, 39, 285, 345, 393
MboII GAAGA 1 cut(s) 524
MluCI AATT 2 cut(s) 125, 516
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 2 cut(s) 189, 204
MnlI CCTC 6 cut(s) 53, 56, 59, 108, 155, 309
MspA1I CMGCKG 1 cut(s) 141
MspI CCGG 2 cut(s) 152, 436
Mva1269I GAATGC 2 cut(s) 303, 489
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 1 cut(s) 138
NdeII GATC 5 cut(s) 33, 39, 285, 345, 393
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 2 cut(s) 3, 446
PcsI WCGNNNNNNNCGW 3 cut(s) 192, 281, 339
PctI GAATGC 2 cut(s) 303, 489
PfeI GAWTC 1 cut(s) 192
Pfl23II CGTACG 1 cut(s) 276
PkrI GCNGC 2 cut(s) 143, 363
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 276
PspLI CGTACG 1 cut(s) 276
PspPI GGNCC 1 cut(s) 310
PstI CTGCAG 1 cut(s) 228
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SacII CCGCGG 1 cut(s) 142
SatI GCNGC 2 cut(s) 142, 362
Sau3AI GATC 5 cut(s) 33, 39, 285, 345, 393
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 1 cut(s) 86
SetI ASST 8 cut(s) 218, 225, 273, 278, 312, 320, 411, 498
SfaNI GCATC 2 cut(s) 164, 513
SfcI CTRYAG 1 cut(s) 224
Sfr303I CCGCGG 1 cut(s) 142
SgrBI CCGCGG 1 cut(s) 142
SinI GGWCC 1 cut(s) 310
Sse9I AATT 2 cut(s) 125, 516
SsiI CCGC 3 cut(s) 139, 141, 362
TaaI ACNGT 2 cut(s) 106, 388
TaiI ACGT 1 cut(s) 278
TaqI TCGA 2 cut(s) 231, 354
TaqII GACCGA 2 cut(s) 148, 407
TasI AATT 2 cut(s) 125, 516
TauI GCSGC 2 cut(s) 144, 364
TfiI GAWTC 1 cut(s) 192
TscAI CASTG 3 cut(s) 111, 391, 513
TseFI GTSAC 2 cut(s) 3, 446
Tsp45I GTSAC 2 cut(s) 3, 446
TspGWI ACGGA 2 cut(s) 49, 201
TspRI CASTG 3 cut(s) 111, 391, 513
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.