AT5G48370

thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003743
Physical Location & Seq
Reverse (-)
19598486 .. 19600708
2223 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G48370.3

Sequence Viewer

Length: 1317 bp
ATGAATTCCCCAAGACCCATCTCCGTCGTCTCCACCTTCGCTTCACCATCCTCCACCTCTGACCCAACCCGAAAACCCTTAAGCTTATGGCCAGGTATGTACCATTCACCTGTCACCACCGCTCTTTGGGAAGCCAGATCCAAAATCTTCGAATCTCTTCTCGATCCTCCTAAAGACGCTCCACCACAGAGCCAGCTTCTCACCAGAACTCCTTCTCATAGCCGTACCACCATTTTCTACCCTTTCTCCACTGATTTCATTCTCAGAGAGCAATACAGAGATCCTTGGAACGAGGTCCGTATCGGGATCTTGCTCGAAGATCTCGATGCTTTAGCCGGCACTATCTCCGTCAAGCATTGTTCTGATGATGATAGTACAACGAGGCCACTCTTGCTTGTTACAGCTTCTGTTCATAAGATTGTCTTAAAGAAGCCGATTTGTGTCGACATTGATCTCAAGATTGTTGCTTCTGTCATTTGGGTTGGTCGTTCATCTATCGAGATTCAACTCGAAGTTATGCAATCCGAATTGAAAGATGTCAAGGCTTCTTCAGATTCTGTTGCTTTAACTGCAAATTTCATTTTTGTGGCGCGTGATTCTAAGACTGGTAAAGCTGCTCCTATCAACCGTCTTTCTCCTGAAACCGAGGTTGAGAAACTTCTCTTTGAGGAAGCTGAAGCTAGGAATAACTTAAGGAAGAAGAAGAGAGGAGGTGACAGAAGGGAATTTGATCACGGGGAGTGTAAGAAGCTTGAGGCTTGGTTGGCTGAAGGAAGGATTTTCTCTGACATGCCAGCTCTTGCTGACAGAAACAGCATTCTTCTTAAAGACACTCGTCTTGAGAATTCGCTGATATGCCAACCGCAGCAGAGGAACATCCATGGTAGGATCTTTGGAGGGTTTTTAATGCATAGAGCATTTGAGTTGGCTTTCTCTACTGCTTATACGTTTGCGGGTCTAGTGCCTTACTTCTTAGAAGTTGATCACGTCGATTTCCTAAGACCGGTGGACGTCGGGGATTTCTTACGTTTCAAATCCTGTGTTCTTTACACTCAACTGGATAAACAGGATTGTCCGCTCATCAATATCGAAGTTGTTGCTCATGTTACAAGTCCAGAGATTCGCTCCAGTGAGGTTTCAAATACATTCTACTTCAAGTTCACTGTAAGGCCAGAGGCAAAGGCCAGAAACAATGGGTTTAAACTTCGAAATGTAGTTCCTGCCACAGAGGAAGAAGCTCGTCATATCCTCGAGCGCATGGATGCAGAAGCTTTGAAGTCAAGCAAACAACAATGTGTAGGCACAATTCTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

438

Amino Acids

49.44

Weight (kDa)

7.14

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1014
AccBSI CCGCTC 2 cut(s) 122, 1078
AccI GTMKAC 1 cut(s) 444
AccII CGCG 1 cut(s) 592
AciI CCGC 4 cut(s) 120, 863, 953, 1076
AclWI GGATC 5 cut(s) 132, 158, 275, 314, 896
AcoI YGGCCR 1 cut(s) 89
AcsI RAATTY 4 cut(s) 4, 574, 725, 844
AcuI CTGAAG 4 cut(s) 534, 696, 789, 1295
AcyI GRCGYC 1 cut(s) 1011
AfaI GTAC 3 cut(s) 101, 226, 376
AfiI CCNNNNNNNGG 2 cut(s) 126, 1003
AflII CTTAAG 2 cut(s) 79, 691
AgeI ACCGGT 1 cut(s) 1003
AgsI TTSAA 6 cut(s) 506, 532, 1033, 1140, 1156, 1276
AjiI CACGTC 1 cut(s) 988
AjnI CCWGG 1 cut(s) 91
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 5 cut(s) 132, 158, 275, 314, 896
AlwNI CAGNNNCTG 2 cut(s) 407, 557
Ama87I CYCGRG 1 cut(s) 1250
AoxI GGCC 4 cut(s) 89, 383, 1169, 1182
ApeKI GCWGC 2 cut(s) 614, 865
ApoI RAATTY 4 cut(s) 4, 574, 725, 844
AsiGI ACCGGT 1 cut(s) 1003
Asp700I GAANNNNTTC 2 cut(s) 156, 211
AspLEI GCGC 2 cut(s) 592, 1257
AspS9I GGNCC 1 cut(s) 295
AsuHPI GGTGA 5 cut(s) 36, 99, 106, 193, 725
AsuII TTCGAA 2 cut(s) 150, 1207
AvaI CYCGRG 1 cut(s) 1250
AvaII GGWCC 1 cut(s) 295
BalI TGGCCA 1 cut(s) 91
BbvI GCAGC 2 cut(s) 601, 877
BccI CCATC 2 cut(s) 26, 55
BceAI ACGGC 1 cut(s) 207
BcgI CGANNNNNNTGC 2 cut(s) 1079, 1113
BciT130I CCWGG 1 cut(s) 93
BclI TGATCA 2 cut(s) 730, 982
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 2 cut(s) 681, 959
BfrI CTTAAG 2 cut(s) 79, 691
BglII AGATCT 1 cut(s) 319
BisI GCNGC 2 cut(s) 615, 866
BlsI GCNGC 2 cut(s) 616, 867
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 1250
BmgBI CACGTC 1 cut(s) 988
BmgT120I GGNCC 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 316, 1252
BoxI GACNNNNGTC 1 cut(s) 834
BplI GAGNNNNNCTC 2 cut(s) 1109, 1141
BpmI CTGGAG 1 cut(s) 1111
Bpu14I TTCGAA 2 cut(s) 150, 1207
BpuEI CTTGAG 3 cut(s) 440, 773, 860
BsaHI GRCGYC 1 cut(s) 1011
BsaJI CCNNGG 3 cut(s) 284, 645, 880
BsaWI WCCGGW 1 cut(s) 1003
BsaXI ACNNNNNCTCC 4 cut(s) 193, 223, 230, 260
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 1003
Bse118I RCCGGY 2 cut(s) 335, 1003
Bse1I ACTGG 3 cut(s) 610, 1062, 1128
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 3 cut(s) 284, 645, 880
BseGI GGATG 3 cut(s) 47, 876, 1267
BseLI CCNNNNNNNGG 2 cut(s) 126, 1003
BseMII CTCAG 1 cut(s) 277
BseNI ACTGG 3 cut(s) 610, 1062, 1128
BseRI GAGGAG 1 cut(s) 723
BseXI GCAGC 2 cut(s) 601, 877
Bsh1236I CGCG 1 cut(s) 592
BshFI GGCC 4 cut(s) 91, 385, 1171, 1184
BshTI ACCGGT 1 cut(s) 1003
BsiHKCI CYCGRG 1 cut(s) 1250
BsiSI CCGG 2 cut(s) 336, 1004
BslI CCNNNNNNNGG 2 cut(s) 126, 1003
BsmAI GTCTC 1 cut(s) 34
BsmBI CGTCTC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 816
BsnI GGCC 4 cut(s) 91, 385, 1171, 1184
BsoBI CYCGRG 1 cut(s) 1250
Bsp119I TTCGAA 2 cut(s) 150, 1207
Bsp143I GATC 9 cut(s) 137, 163, 280, 306, 319, 451, 730, 888, 982
Bsp19I CCATGG 1 cut(s) 880
BspACI CCGC 4 cut(s) 120, 863, 953, 1076
BspANI GGCC 4 cut(s) 91, 385, 1171, 1184
BspCNI CTCAG 1 cut(s) 276
BspFNI CGCG 1 cut(s) 592
BspPI GGATC 5 cut(s) 132, 158, 275, 314, 896
BspT104I TTCGAA 2 cut(s) 150, 1207
BspTI CTTAAG 2 cut(s) 79, 691
BsrBI CCGCTC 2 cut(s) 122, 1078
BsrFI RCCGGY 2 cut(s) 335, 1003
BsrI ACTGG 3 cut(s) 610, 1062, 1128
BssAI RCCGGY 2 cut(s) 335, 1003
BssECI CCNNGG 3 cut(s) 284, 645, 880
BssMI GATC 9 cut(s) 137, 163, 280, 306, 319, 451, 730, 888, 982
BssNI GRCGYC 1 cut(s) 1011
BssT1I CCWWGG 2 cut(s) 284, 880
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 629, 1165
Bst6I CTCTTC 2 cut(s) 162, 698
BstACI GRCGYC 1 cut(s) 1011
BstAFI CTTAAG 2 cut(s) 79, 691
BstBI TTCGAA 2 cut(s) 150, 1207
BstC8I GCNNGC 3 cut(s) 194, 337, 795
BstDEI CTNAG 4 cut(s) 263, 600, 973, 998
BstDSI CCRYGG 1 cut(s) 880
BstF5I GGATG 3 cut(s) 47, 876, 1267
BstFNI CGCG 1 cut(s) 592
BstHHI GCGC 2 cut(s) 592, 1257
BstKTI GATC 9 cut(s) 140, 166, 283, 309, 322, 454, 733, 891, 985
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 9 cut(s) 137, 163, 280, 306, 319, 451, 730, 888, 982
BstMWI GCNNNNNNNGC 3 cut(s) 391, 569, 764
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 1 cut(s) 793
BstPAI GACNNNNGTC 1 cut(s) 834
BstSCI CCNGG 1 cut(s) 91
BstUI CGCG 1 cut(s) 592
BstV1I GCAGC 2 cut(s) 601, 877
BstX2I RGATCY 5 cut(s) 137, 280, 306, 319, 888
BstYI RGATCY 5 cut(s) 137, 280, 306, 319, 888
BsuRI GGCC 4 cut(s) 91, 385, 1171, 1184
BtgI CCRYGG 1 cut(s) 880
BtrI CACGTC 1 cut(s) 988
BtsCI GGATG 3 cut(s) 47, 876, 1267
BtsIMutI CAGTG 3 cut(s) 249, 1135, 1161
Cac8I GCNNGC 3 cut(s) 194, 337, 795
CaiI CAGNNNCTG 2 cut(s) 407, 557
CfoI GCGC 2 cut(s) 592, 1257
Cfr10I RCCGGY 2 cut(s) 335, 1003
Cfr13I GGNCC 1 cut(s) 295
CseI GACGC 1 cut(s) 185
Csp6I GTAC 3 cut(s) 100, 225, 375
CspAI ACCGGT 1 cut(s) 1003
CviAII CATG 4 cut(s) 790, 881, 1103, 1258
CviQI GTAC 3 cut(s) 100, 225, 375
DdeI CTNAG 4 cut(s) 263, 600, 973, 998
DpnI GATC 9 cut(s) 139, 165, 282, 308, 321, 453, 732, 890, 984
DpnII GATC 9 cut(s) 137, 163, 280, 306, 319, 451, 730, 888, 982
DraI TTTAAA 1 cut(s) 1201
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 2 cut(s) 162, 698
EarI CTCTTC 2 cut(s) 162, 698
Eco130I CCWWGG 2 cut(s) 284, 880
Eco47I GGWCC 1 cut(s) 295
Eco57I CTGAAG 4 cut(s) 534, 696, 789, 1295
Eco88I CYCGRG 1 cut(s) 1250
EcoRI GAATTC 2 cut(s) 4, 844
EcoRII CCWGG 1 cut(s) 91
EcoT14I CCWWGG 2 cut(s) 284, 880
EcoT22I ATGCAT 1 cut(s) 912
ErhI CCWWGG 2 cut(s) 284, 880
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 4 cut(s) 793, 884, 1106, 1261
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FatI CATG 4 cut(s) 789, 880, 1102, 1257
FauI CCCGC 1 cut(s) 946
FbaI TGATCA 2 cut(s) 730, 982
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 2 cut(s) 615, 866
FokI GGATG 3 cut(s) 34, 863, 1274
Fsp4HI GCNGC 2 cut(s) 615, 866
FspBI CTAG 2 cut(s) 681, 959
GlaI GCGC 2 cut(s) 591, 1256
GluI GCNGC 2 cut(s) 615, 866
GsuI CTGGAG 1 cut(s) 1111
HaeIII GGCC 4 cut(s) 91, 385, 1171, 1184
HapII CCGG 2 cut(s) 336, 1004
HgaI GACGC 1 cut(s) 185
HhaI GCGC 2 cut(s) 592, 1257
Hin1I GRCGYC 1 cut(s) 1011
Hin1II CATG 4 cut(s) 793, 884, 1106, 1261
Hin6I GCGC 2 cut(s) 590, 1255
HinP1I GCGC 2 cut(s) 590, 1255
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HindIII AAGCTT 3 cut(s) 82, 749, 1269
HinfI GANTC 5 cut(s) 152, 502, 554, 596, 1120
HpaII CCGG 2 cut(s) 336, 1004
HphI GGTGA 5 cut(s) 36, 99, 106, 193, 725
Hpy166II GTNNAC 3 cut(s) 445, 1009, 1161
Hpy188I TCNGA 6 cut(s) 61, 266, 364, 526, 553, 787
Hpy188III TCNNGA 8 cut(s) 161, 304, 323, 457, 499, 638, 839, 1115
Hpy8I GTNNAC 3 cut(s) 445, 1009, 1161
Hpy99I CGWCG 3 cut(s) 29, 992, 1016
HpyAV CCTTC 5 cut(s) 46, 222, 714, 764, 768
HpyCH4III ACNGT 2 cut(s) 629, 1165
HpyCH4IV ACGT 4 cut(s) 947, 987, 1011, 1027
HpyCH4V TGCA 4 cut(s) 520, 572, 910, 1265
HpyF10VI GCNNNNNNNGC 3 cut(s) 391, 569, 764
HpyF3I CTNAG 4 cut(s) 263, 600, 973, 998
HpySE526I ACGT 4 cut(s) 947, 987, 1011, 1027
Hsp92I GRCGYC 1 cut(s) 1011
Hsp92II CATG 4 cut(s) 793, 884, 1106, 1261
HspAI GCGC 2 cut(s) 590, 1255
KroI GCCGGC 1 cut(s) 335
KroNI GCCGGC 1 cut(s) 337
Ksp22I TGATCA 2 cut(s) 730, 982
Kzo9I GATC 9 cut(s) 137, 163, 280, 306, 319, 451, 730, 888, 982
LmnI GCTCC 3 cut(s) 184, 622, 1130
Lsp1109I GCAGC 2 cut(s) 601, 877
LweI GCATC 2 cut(s) 316, 1252
MaeI CTAG 2 cut(s) 681, 959
MaeII ACGT 4 cut(s) 947, 987, 1011, 1027
MaeIII GTNAC 4 cut(s) 112, 397, 713, 1105
MalI GATC 9 cut(s) 139, 165, 282, 308, 321, 453, 732, 890, 984
MbiI CCGCTC 2 cut(s) 122, 1078
MboI GATC 9 cut(s) 137, 163, 280, 306, 319, 451, 730, 888, 982
MflI RGATCY 5 cut(s) 137, 280, 306, 319, 888
MlsI TGGCCA 1 cut(s) 91
MluCI AATT 6 cut(s) 4, 527, 574, 725, 844, 1305
MluNI TGGCCA 1 cut(s) 91
Mox20I TGGCCA 1 cut(s) 91
Mph1103I ATGCAT 1 cut(s) 912
MroNI GCCGGC 1 cut(s) 335
MroXI GAANNNNTTC 2 cut(s) 156, 211
MscI TGGCCA 1 cut(s) 91
MseI TTAA 7 cut(s) 80, 425, 566, 692, 825, 905, 1200
MslI CAYNNNNRTG 1 cut(s) 584
Msp20I TGGCCA 1 cut(s) 91
MspCI CTTAAG 2 cut(s) 79, 691
MspI CCGG 2 cut(s) 336, 1004
MspR9I CCNGG 1 cut(s) 93
MssI GTTTAAAC 1 cut(s) 1201
Mva1269I GAATGC 1 cut(s) 816
MvaI CCWGG 1 cut(s) 93
MvnI CGCG 1 cut(s) 592
MwoI GCNNNNNNNGC 3 cut(s) 391, 569, 764
NaeI GCCGGC 1 cut(s) 337
NcoI CCATGG 1 cut(s) 880
NdeII GATC 9 cut(s) 137, 163, 280, 306, 319, 451, 730, 888, 982
NgoMIV GCCGGC 1 cut(s) 335
NlaIII CATG 4 cut(s) 793, 884, 1106, 1261
NmuCI GTSAC 2 cut(s) 112, 713
NsiI ATGCAT 1 cut(s) 912
NspI RCATGY 1 cut(s) 793
NspV TTCGAA 2 cut(s) 150, 1207
PaeR7I CTCGAG 1 cut(s) 1250
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PctI GAATGC 1 cut(s) 816
PdiI GCCGGC 1 cut(s) 337
PdmI GAANNNNTTC 2 cut(s) 156, 211
PfeI GAWTC 5 cut(s) 152, 502, 554, 596, 1120
PinAI ACCGGT 1 cut(s) 1003
PkrI GCNGC 2 cut(s) 616, 867
PmeI GTTTAAAC 1 cut(s) 1201
PshAI GACNNNNGTC 1 cut(s) 834
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspPI GGNCC 1 cut(s) 295
PspXI VCTCGAGB 1 cut(s) 1250
PstNI CAGNNNCTG 2 cut(s) 407, 557
PsuI RGATCY 5 cut(s) 137, 280, 306, 319, 888
RsaI GTAC 3 cut(s) 101, 226, 376
RsaNI GTAC 3 cut(s) 100, 225, 375
RseI CAYNNNNRTG 1 cut(s) 584
SalI GTCGAC 1 cut(s) 443
SaqAI TTAA 7 cut(s) 80, 425, 566, 692, 825, 905, 1200
SatI GCNGC 2 cut(s) 615, 866
Sau3AI GATC 9 cut(s) 137, 163, 280, 306, 319, 451, 730, 888, 982
Sau96I GGNCC 1 cut(s) 295
ScrFI CCNGG 1 cut(s) 93
SfaNI GCATC 2 cut(s) 316, 1252
Sfr274I CTCGAG 1 cut(s) 1250
SfuI TTCGAA 2 cut(s) 150, 1207
SinI GGWCC 1 cut(s) 295
SlaI CTCGAG 1 cut(s) 1250
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 6 cut(s) 79, 455, 691, 752, 839, 1250
SmoI CTYRAG 6 cut(s) 79, 455, 691, 752, 839, 1250
Sse9I AATT 6 cut(s) 4, 527, 574, 725, 844, 1305
SsiI CCGC 4 cut(s) 120, 863, 953, 1076
SspMI CTAG 2 cut(s) 681, 959
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 2 cut(s) 284, 880
TaaI ACNGT 2 cut(s) 629, 1165
TaiI ACGT 4 cut(s) 950, 990, 1014, 1030
TasI AATT 6 cut(s) 4, 527, 574, 725, 844, 1305
TatI WGTACW 1 cut(s) 374
TfiI GAWTC 5 cut(s) 152, 502, 554, 596, 1120
Tru1I TTAA 7 cut(s) 80, 425, 566, 692, 825, 905, 1200
Tru9I TTAA 7 cut(s) 80, 425, 566, 692, 825, 905, 1200
TscAI CASTG 3 cut(s) 256, 1135, 1168
TseFI GTSAC 2 cut(s) 112, 713
TseI GCWGC 2 cut(s) 614, 865
Tsp45I GTSAC 2 cut(s) 112, 713
TspDTI ATGAA 5 cut(s) 17, 247, 401, 480, 568
TspGWI ACGGA 3 cut(s) 13, 287, 337
TspRI CASTG 3 cut(s) 256, 1135, 1168
Vha464I CTTAAG 2 cut(s) 79, 691
VpaK11BI GGWCC 1 cut(s) 295
XapI RAATTY 4 cut(s) 4, 574, 725, 844
XceI RCATGY 1 cut(s) 793
XhoI CTCGAG 1 cut(s) 1250
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 2 cut(s) 156, 211
XspI CTAG 2 cut(s) 681, 959
ZraI GACGTC 1 cut(s) 1012
Zsp2I ATGCAT 1 cut(s) 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.