Rorug06G0144800

Acyl-coenzyme A thioesterase 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003743
Physical Location & Seq
Forward (+)
20628464 .. 20630131
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0144800.1

Sequence Viewer

Length: 501 bp
ATGGCTGCAGGAAGAATTAAAACTTACTTGGAAAGGGTTGATGCTCGGCTAGGTGGAGGAATCAAGAAAAAATCTGTGACCCTTATATCTGGCCATGAGAAGTCGGCCAGGACAAGGACAGATCTGTGTCACCGATTCTGTGTCATTAGCCAACTCCCAAAGGAAGATGGAGCAAAGGAGAAGCAAAAGGTCATGTACCTTGATTTCTCAAGAGCGTGTAGTTTTAGGCCAGATAAAATGCTCGAGATAGCAAAGAGCTTTCCCTTGCATGGAGATAATTTGTTGAAAAATATGACGTATGGTAGAATTTCGAAAATTTCTGCTAAAGATATTTATAAGGAACAAAAGAAGTTATATGATGAAGCAAAGGCAAAAATAAGAGCAGATGGAAGGTTTGGTCTACTGGTAGTGAACGATGCTCCAAACTTAAATATAAGAGAACTTCCACTATGTCAAAAAGGCCTTCAGACGACAGAACTGTTCTGTCGTCTGAACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

166

Amino Acids

18.86

Weight (kDa)

9.66

Isoelectric Point (pI)

29.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad51 PF08423 6 - 140 2.9e-19 Rad51
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AccI GTMKAC 1 cut(s) 400
AcoI YGGCCR 2 cut(s) 91, 105
AcsI RAATTY 2 cut(s) 306, 315
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 114, 269
AgsI TTSAA 1 cut(s) 286
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 1 cut(s) 258
AluI AGCT 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 4 cut(s) 91, 105, 227, 460
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 306, 315
AsuHPI GGTGA 1 cut(s) 122
AsuII TTCGAA 1 cut(s) 311
AvaI CYCGRG 1 cut(s) 242
BalI TGGCCA 1 cut(s) 93
BccI CCATC 2 cut(s) 161, 380
BciT130I CCWGG 1 cut(s) 109
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 121
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 242
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 2 cut(s) 31, 406
Bpu14I TTCGAA 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 193
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 269
Bse1I ACTGG 1 cut(s) 408
BseBI CCWGG 1 cut(s) 109
BseLI CCNNNNNNNGG 2 cut(s) 114, 269
BseNI ACTGG 1 cut(s) 408
BshFI GGCC 4 cut(s) 93, 107, 229, 462
BsiHKCI CYCGRG 1 cut(s) 242
BslI CCNNNNNNNGG 2 cut(s) 114, 269
BsnI GGCC 4 cut(s) 93, 107, 229, 462
BsoBI CYCGRG 1 cut(s) 242
Bsp119I TTCGAA 1 cut(s) 311
Bsp143I GATC 1 cut(s) 121
BspANI GGCC 4 cut(s) 93, 107, 229, 462
BspMAI CTGCAG 1 cut(s) 10
BspT104I TTCGAA 1 cut(s) 311
BsrI ACTGG 1 cut(s) 408
BssMI GATC 1 cut(s) 121
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 480
BstBI TTCGAA 1 cut(s) 311
BstKTI GATC 1 cut(s) 124
BstMBI GATC 1 cut(s) 121
BstNI CCWGG 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 6
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 4 cut(s) 93, 107, 229, 462
Csp6I GTAC 1 cut(s) 196
CviAII CATG 3 cut(s) 95, 193, 269
CviJI RGCY 8 cut(s) 5, 49, 93, 107, 150, 229, 258, 462
CviKI_1 RGCY 8 cut(s) 5, 49, 93, 107, 150, 229, 258, 462
CviQI GTAC 1 cut(s) 196
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
EaeI YGGCCR 2 cut(s) 91, 105
Eco147I AGGCCT 1 cut(s) 462
Eco57I CTGAAG 1 cut(s) 449
Eco88I CYCGRG 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 107
FaeI CATG 3 cut(s) 98, 196, 272
FatI CATG 3 cut(s) 94, 192, 268
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 1 cut(s) 6
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 50
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 4 cut(s) 93, 107, 229, 462
Hin1II CATG 3 cut(s) 98, 196, 272
HinfI GANTC 2 cut(s) 60, 135
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 401, 412
Hpy188I TCNGA 2 cut(s) 468, 492
Hpy188III TCNNGA 3 cut(s) 64, 210, 244
Hpy8I GTNNAC 2 cut(s) 401, 412
HpyAV CCTTC 2 cut(s) 384, 473
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 2 cut(s) 8, 268
HpySE526I ACGT 1 cut(s) 296
Hsp92II CATG 3 cut(s) 98, 196, 272
Kzo9I GATC 1 cut(s) 121
LmnI GCTCC 2 cut(s) 170, 424
LpnPI CCDG 5 cut(s) 75, 94, 121, 243, 389
LweI GCATC 2 cut(s) 31, 406
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 2 cut(s) 76, 128
MalI GATC 1 cut(s) 123
MboI GATC 1 cut(s) 121
MboII GAAGA 2 cut(s) 24, 176
MflI RGATCY 1 cut(s) 121
MlsI TGGCCA 1 cut(s) 93
MluCI AATT 4 cut(s) 15, 277, 306, 315
MluNI TGGCCA 1 cut(s) 93
MnlI CCTC 1 cut(s) 50
Mox20I TGGCCA 1 cut(s) 93
MscI TGGCCA 1 cut(s) 93
MseI TTAA 2 cut(s) 18, 428
Msp20I TGGCCA 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 109
MvaI CCWGG 1 cut(s) 109
NdeII GATC 1 cut(s) 121
NlaIII CATG 3 cut(s) 98, 196, 272
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 2 cut(s) 76, 128
NspV TTCGAA 1 cut(s) 311
PaeR7I CTCGAG 1 cut(s) 242
PceI AGGCCT 1 cut(s) 462
PfeI GAWTC 2 cut(s) 60, 135
PkrI GCNGC 1 cut(s) 7
PsiI TTATAA 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SaqAI TTAA 2 cut(s) 18, 428
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 121
ScrFI CCNGG 1 cut(s) 109
SetI ASST 6 cut(s) 55, 192, 201, 260, 299, 395
SfaNI GCATC 2 cut(s) 31, 406
SfcI CTRYAG 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 242
SfuI TTCGAA 1 cut(s) 311
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 2 cut(s) 208, 242
SmoI CTYRAG 2 cut(s) 208, 242
Sse9I AATT 4 cut(s) 15, 277, 306, 315
SseBI AGGCCT 1 cut(s) 462
SspMI CTAG 1 cut(s) 50
StuI AGGCCT 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 107
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 1 cut(s) 299
TaqI TCGA 2 cut(s) 243, 311
TasI AATT 4 cut(s) 15, 277, 306, 315
TfiI GAWTC 2 cut(s) 60, 135
Tru1I TTAA 2 cut(s) 18, 428
Tru9I TTAA 2 cut(s) 18, 428
TseFI GTSAC 2 cut(s) 76, 128
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 76, 128
TspDTI ATGAA 1 cut(s) 375
XapI RAATTY 2 cut(s) 306, 315
XhoI CTCGAG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 400
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.