AT5G57655

Xylose isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003254
Physical Location & Seq
Forward (+)
23346675 .. 23350065
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57655.2

Sequence Viewer

Length: 864 bp
ATGAAGAAAGTTGAGTTTTTTATGCTCCTTCTCTGCTTCATCGCAGCGTCATCTCTAGTGTCTGCTGATCCACCAACATGTCCTGCTGATTTGGGAGGCAAGTGTAGTGATTCTGATGACTGGCAAGGTGATTTCTTCCCTGAAATCCCCAAGATTAAGTATGAGGGACCTTCGAGTAAGAACCCACTTGCTTACAGGTGGTATAACGCTGAAGAAGAGATTCTTGGGAAGAAAATGAAGGATTGGTTTAGATTCAGTGTTGCGTTTTGGCATACTTTCCGTGGGACTGGAGGTGATCCATTTGGTGCTGCTACTAAGTATTGGCCTTGGGAAGATGGTACTAACTCTGTGTCTATGGCTAAGAGAAGAATGAGAGCTAACTTTGAGTTCTTGAAGAAACTTGGAGTTGATTGGTGGTGTTTTCATGACAGAGACATAGCACCTGATGGCACTACACTTGAGGAATCTAACAAAAACTTGGATGAAGTTATCGAACTCGCCAAAGAACTCCAAAAGGGGAGCAAAATCAAACCATTGTGGGGAACAGCTCAGCTTTTCTTGCATCCTCGTTATATGCACGGAGGAGCGACCAGCTCTGAAGTTGGAGTGTATGCTTACGCTGCTGCTCAGGTGAAGAAAGCCATGGAGGTAACACATTACTTAGGTGGTGAAAACTACGTTTTTTGGGGTGGTCGTGAAGGTTATCAGACACTCTTGAACACTGATATGGGAAGAGAGCTTGATCATCTAGCCAGGTTCTTTGAAGCTGCTGTTGCTTACAAGAAGAAGATTGGATTCAAAGGTAAGTCTACTGCTTCTTTTTCTTGTCCTGTATTCAGTCTTATGGGAGTTATTGCACAATAA

Protein Analysis

287

Amino Acids

32.41

Weight (kDa)

7.62

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP_endonuc_2 PF01261 128 - 339 8.5e-07 Xylose isomerase-like TIM barrel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 809
AclWI GGATC 2 cut(s) 62, 290
AcuI CTGAAG 2 cut(s) 231, 618
AfaI GTAC 1 cut(s) 340
AfiI CCNNNNNNNGG 1 cut(s) 539
AflIII ACRYGT 1 cut(s) 77
AgsI TTSAA 4 cut(s) 394, 718, 764, 799
AjnI CCWGG 1 cut(s) 752
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AluBI AGCT 6 cut(s) 377, 548, 553, 594, 739, 767
AluI AGCT 6 cut(s) 377, 548, 553, 594, 739, 767
Alw26I GTCTC 1 cut(s) 426
AlwI GGATC 2 cut(s) 62, 290
AoxI GGCC 1 cut(s) 323
ApeKI GCWGC 5 cut(s) 44, 308, 620, 623, 767
Asp700I GAANNNNTTC 1 cut(s) 219
AspS9I GGNCC 1 cut(s) 167
AsuHPI GGTGA 4 cut(s) 140, 305, 643, 680
AvaII GGWCC 1 cut(s) 167
BbvI GCAGC 5 cut(s) 56, 295, 607, 610, 754
BccI CCATC 2 cut(s) 329, 440
BciT130I CCWGG 1 cut(s) 754
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 2 cut(s) 56, 749
BisI GCNGC 5 cut(s) 45, 309, 621, 624, 768
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 5 cut(s) 46, 310, 622, 625, 769
Bme1390I CCNGG 1 cut(s) 754
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 754
BmsI GCATC 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 627
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 479
BsaJI CCNNGG 3 cut(s) 280, 326, 642
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse1I ACTGG 2 cut(s) 125, 292
BseBI CCWGG 1 cut(s) 754
BseDI CCNNGG 3 cut(s) 280, 326, 642
BseGI GGATG 2 cut(s) 487, 562
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMII CTCAG 2 cut(s) 563, 641
BseNI ACTGG 2 cut(s) 125, 292
BseRI GAGGAG 1 cut(s) 597
BseXI GCAGC 5 cut(s) 56, 295, 607, 610, 754
BshFI GGCC 1 cut(s) 325
BslFI GGGAC 2 cut(s) 180, 298
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 1 cut(s) 426
BsmFI GGGAC 2 cut(s) 180, 298
BsnI GGCC 1 cut(s) 325
Bsp143I GATC 3 cut(s) 67, 295, 742
Bsp1720I GCTNAGC 1 cut(s) 549
Bsp19I CCATGG 1 cut(s) 642
BspANI GGCC 1 cut(s) 325
BspCNI CTCAG 2 cut(s) 562, 640
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 2 cut(s) 62, 290
BsrI ACTGG 2 cut(s) 125, 292
BssECI CCNNGG 3 cut(s) 280, 326, 642
BssMI GATC 3 cut(s) 67, 295, 742
BssT1I CCWWGG 2 cut(s) 326, 642
Bst2UI CCWGG 1 cut(s) 754
Bst6I CTCTTC 2 cut(s) 210, 727
BstDEI CTNAG 5 cut(s) 315, 360, 549, 627, 661
BstDSI CCRYGG 2 cut(s) 280, 642
BstF5I GGATG 2 cut(s) 487, 562
BstKTI GATC 3 cut(s) 70, 298, 745
BstMAI GTCTC 1 cut(s) 426
BstMBI GATC 3 cut(s) 67, 295, 742
BstMWI GCNNNNNNNGC 3 cut(s) 559, 620, 773
BstNI CCWGG 1 cut(s) 754
BstNSI RCATGY 1 cut(s) 81
BstSCI CCNGG 1 cut(s) 752
BstV1I GCAGC 5 cut(s) 56, 295, 607, 610, 754
BsuRI GGCC 1 cut(s) 325
BtgI CCRYGG 2 cut(s) 280, 642
BtgZI GCGATG 1 cut(s) 25
BtsCI GGATG 2 cut(s) 487, 562
BtsIMutI CAGTG 2 cut(s) 262, 720
CciI TCATGA 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 167
CseI GACGC 1 cut(s) 36
Csp6I GTAC 1 cut(s) 339
CviAII CATG 3 cut(s) 78, 425, 643
CviQI GTAC 1 cut(s) 339
DdeI CTNAG 5 cut(s) 315, 360, 549, 627, 661
DpnI GATC 3 cut(s) 69, 297, 744
DpnII GATC 3 cut(s) 67, 295, 742
Eam1104I CTCTTC 2 cut(s) 210, 727
EarI CTCTTC 2 cut(s) 210, 727
Eco130I CCWWGG 2 cut(s) 326, 642
Eco47I GGWCC 1 cut(s) 167
Eco57I CTGAAG 2 cut(s) 231, 618
EcoO109I RGGNCCY 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 752
EcoT14I CCWWGG 2 cut(s) 326, 642
ErhI CCWWGG 2 cut(s) 326, 642
FaeI CATG 3 cut(s) 81, 428, 646
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FaqI GGGAC 2 cut(s) 180, 298
FatI CATG 3 cut(s) 77, 424, 642
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 1 cut(s) 809
Fnu4HI GCNGC 5 cut(s) 45, 309, 621, 624, 768
FokI GGATG 2 cut(s) 494, 549
Fsp4HI GCNGC 5 cut(s) 45, 309, 621, 624, 768
FspBI CTAG 2 cut(s) 56, 749
GluI GCNGC 5 cut(s) 45, 309, 621, 624, 768
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 1 cut(s) 325
HgaI GACGC 1 cut(s) 36
Hin1II CATG 3 cut(s) 81, 428, 646
HinfI GANTC 5 cut(s) 110, 220, 252, 464, 795
HphI GGTGA 4 cut(s) 140, 305, 643, 680
Hpy166II GTNNAC 1 cut(s) 810
Hpy188I TCNGA 3 cut(s) 115, 598, 708
Hpy188III TCNNGA 4 cut(s) 391, 425, 695, 715
Hpy8I GTNNAC 1 cut(s) 810
HpyAV CCTTC 4 cut(s) 38, 180, 232, 692
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 3 cut(s) 562, 577, 857
HpyF10VI GCNNNNNNNGC 3 cut(s) 559, 620, 773
HpyF3I CTNAG 5 cut(s) 315, 360, 549, 627, 661
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 3 cut(s) 81, 428, 646
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 3 cut(s) 67, 295, 742
LmnI GCTCC 3 cut(s) 30, 519, 584
Lsp1109I GCAGC 5 cut(s) 56, 295, 607, 610, 754
LweI GCATC 1 cut(s) 571
MaeI CTAG 2 cut(s) 56, 749
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 1 cut(s) 649
MalI GATC 3 cut(s) 69, 297, 744
MboI GATC 3 cut(s) 67, 295, 742
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 7 cut(s) 89, 157, 284, 454, 575, 576, 640
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 1 cut(s) 156
MslI CAYNNNNRTG 2 cut(s) 76, 725
MspR9I CCNGG 1 cut(s) 754
MvaI CCWGG 1 cut(s) 754
MwoI GCNNNNNNNGC 3 cut(s) 559, 620, 773
NcoI CCATGG 1 cut(s) 642
NdeII GATC 3 cut(s) 67, 295, 742
NlaIII CATG 3 cut(s) 81, 428, 646
NlaIV GGNNCC 1 cut(s) 168
NspI RCATGY 1 cut(s) 81
PagI TCATGA 1 cut(s) 424
PciI ACATGT 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 5 cut(s) 110, 220, 252, 464, 795
PkrI GCNGC 5 cut(s) 46, 310, 622, 625, 769
PpuMI RGGWCCY 1 cut(s) 167
PscI ACATGT 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 752
PspGI CCWGG 1 cut(s) 752
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 1 cut(s) 167
PspPPI RGGWCCY 1 cut(s) 167
RsaI GTAC 1 cut(s) 340
RsaNI GTAC 1 cut(s) 339
RseI CAYNNNNRTG 2 cut(s) 76, 725
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 5 cut(s) 45, 309, 621, 624, 768
Sau3AI GATC 3 cut(s) 67, 295, 742
Sau96I GGNCC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 754
SfaNI GCATC 1 cut(s) 571
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 2 cut(s) 76, 725
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SspMI CTAG 2 cut(s) 56, 749
StyD4I CCNGG 1 cut(s) 752
StyI CCWWGG 2 cut(s) 326, 642
TaiI ACGT 1 cut(s) 681
TaqI TCGA 2 cut(s) 173, 492
TfiI GAWTC 5 cut(s) 110, 220, 252, 464, 795
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 262, 727
TseI GCWGC 5 cut(s) 44, 308, 620, 623, 767
TspDTI ATGAA 5 cut(s) 17, 28, 251, 413, 498
TspGWI ACGGA 2 cut(s) 269, 594
TspRI CASTG 2 cut(s) 262, 727
VpaK11BI GGWCC 1 cut(s) 167
XceI RCATGY 1 cut(s) 81
XmiI GTMKAC 1 cut(s) 809
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 2 cut(s) 56, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.