Rh4AG119600

Xylose isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003254
Physical Location & Seq
Forward (+)
25842265 .. 25849383
7119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG119600.1

Sequence Viewer

Length: 1440 bp
ATGATGAAAATGAAGGCAGAGAAGATATTGTTGCTGCTTGTTGGTGTCAGCTTCTTTATCTTTGGAGTGACTGCTGGTCCGCAGACGTGCCCTGCAGATCTTGAGAGTGATTGCAGTGGTTCTGGGGAATGGAAAGGGGAGTTTTTCCCTGAAATTCCCAAAATTAAATATGAGGGCCCCTCTAGCAAGAACCCCCTTGCGTACAAATGGTATAACGCAGACGAGGAGATTCTGGGGAAGAAAATGAAGGATTGGTTGAGGTTTAGTGTTGCATTCTGGCACACATTCCGTGGAACAGGAGCTGACCCATTCGGTGCGCCTACAAAAAATTGGCCTTGGGAGGATGGAACTAACTCTGTTGCTATGGCCAAGAGAAGAATGAAAGCCAACTTTGAGTTCATAAATAAGCTTGGAGTCGATAGGTGGTGTTTCCATGACAGGGATATTGCTCCGGATGGCAAAACCTTGGAGGAAACTAATAAAAACTTGGATGAGGTGGTGGCCCTTGCCAAGGAGCTTCAGGGAACCAAAATTCGACCTTTGTGGGGCACAGCTCAGCTGTTTATGCATCCTCGCTACATGCACGGTGGTGCCACTAGCCCTGAACTAGGTGTATATGCATATGCTGCTGCTCAAGTTAAGAAAGCTATTGAGGTCACGCATTATTTAGGTGGTGAAAATTATGTGTTTTGGGGTGGCCGTGAGGGTTACCAGAGTCTCCTGAATACCGACATGGGACGAGAGCTTGATCATCTGGCAAGGTTTCTTGAAGCTGCTGTTGCTTACAAGAAGAAAATTGGATTCAATGGAACACTGTTGATTGAACCGAAGCCTCAAGAACCTACTAAACACCAGTATGACTGGGATGCTGCAACAACAGCAAATTTCCTGCGAAAATATGGCCTTATAGGAGAATTTAAACTTAACATTGAGTGCAACCATGCCACCCTATCTGGTCACAGCTGTCATCATGAGCTTGAAACTGCAAGACTCAATGGTTTACTGGGAAACATTGATGCAAACACTGGAGATCCTCAAATTGGATGGGATACAGATGAATTTCTCACAGACATTGCAGAGGCAACTCGAGTTATGCTTACTGTGGTAAAAAATGGAGGAGTGGCACCAGGAGGCTTCAACTTTGATGCAAAATTACGGAGGGAAAGCACAGATGTCGAGGATTTGTTCATTGCTCATATCAGTGGAATGGATACCCTGGCTCGTGGACTCCGTAATGTCGCCAAGCTTATTGAGGACGGTTCTTTGGCTGAGCTTGTTCGCAAACGATATGAGAGTTTTGATACAGAGGTTGGGGCCCAGATAGAGGCTGGTAAGGGTGATTTTGAATATCTTGAGAAGAAGGCCATGGAATGGGGTGAACCAAAAGTTCCTTCTGCCAAGCAGGAACTTGCGGAGAGGCATTTCCAGTCTGCTTTGTAG

Protein Analysis

479

Amino Acids

53.63

Weight (kDa)

5.8

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP_endonuc_2 PF01261 162 - 321 4.3e-06 Xylose isomerase-like TIM barrel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 590, 1123
AccB7I CCANNNNNTGG 1 cut(s) 1371
AccIII TCCGGA 1 cut(s) 451
AciI CCGC 2 cut(s) 80, 1412
AclWI GGATC 1 cut(s) 1025
AcoI YGGCCR 2 cut(s) 366, 697
AcsI RAATTY 5 cut(s) 153, 531, 883, 914, 1058
AcuI CTGAAG 1 cut(s) 503
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 7 cut(s) 439, 511, 545, 608, 1040, 1324, 1371
AgsI TTSAA 6 cut(s) 770, 805, 824, 980, 1138, 1346
AhdI GACNNNNNGTC 1 cut(s) 75
AjiI CACGTC 1 cut(s) 87
AjnI CCWGG 2 cut(s) 1126, 1215
AjuI GAANNNNNNNTTGG 4 cut(s) 380, 412, 1375, 1407
AleI CACNNNNGTG 1 cut(s) 588
Alw26I GTCTC 1 cut(s) 722
AlwI GGATC 1 cut(s) 1025
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 1086
Aor13HI TCCGGA 1 cut(s) 451
AoxI GGCC 8 cut(s) 175, 332, 366, 501, 697, 901, 1314, 1362
ApaI GGGCCC 2 cut(s) 179, 1318
ApeKI GCWGC 5 cut(s) 34, 626, 629, 773, 869
ApoI RAATTY 5 cut(s) 153, 531, 883, 914, 1058
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 6 cut(s) 77, 175, 176, 502, 1314, 1315
AsuHPI GGTGA 3 cut(s) 686, 1349, 1388
AvaI CYCGRG 1 cut(s) 1086
AvaII GGWCC 1 cut(s) 77
BaeGI GKGCMC 4 cut(s) 92, 179, 551, 1318
BaeI ACNNNNGTAYC 2 cut(s) 1293, 1326
BalI TGGCCA 1 cut(s) 368
BanI GGYRCC 2 cut(s) 590, 1123
BanII GRGCYC 2 cut(s) 179, 1318
BauI CACGAG 1 cut(s) 1221
BbvI GCAGC 5 cut(s) 21, 613, 616, 760, 856
BccI CCATC 3 cut(s) 338, 449, 1038
BceAI ACGGC 1 cut(s) 684
BcgI CGANNNNNNTGC 2 cut(s) 1156, 1190
BciT130I CCWGG 2 cut(s) 1128, 1217
BciVI GTATCC 2 cut(s) 1042, 1204
BclI TGATCA 1 cut(s) 748
BcoDI GTCTC 1 cut(s) 722
BfaI CTAG 3 cut(s) 183, 597, 608
BfmI CTRYAG 1 cut(s) 93
BfuI GTATCC 2 cut(s) 1042, 1204
BglII AGATCT 1 cut(s) 97
BisI GCNGC 5 cut(s) 35, 627, 630, 774, 870
BlpI GCTNAGC 2 cut(s) 555, 1269
BlsI GCNGC 5 cut(s) 36, 628, 631, 775, 871
Bme1390I CCNGG 2 cut(s) 1128, 1217
Bme18I GGWCC 1 cut(s) 77
BmeRI GACNNNNNGTC 1 cut(s) 75
BmeT110I CYCGRG 1 cut(s) 1086
BmgBI CACGTC 1 cut(s) 87
BmgT120I GGNCC 6 cut(s) 77, 175, 176, 502, 1314, 1315
BmiI GGNNCC 7 cut(s) 177, 178, 526, 592, 1125, 1315, 1316
BmrFI CCNGG 2 cut(s) 1128, 1217
BmrI ACTGGG 2 cut(s) 871, 1013
BmsI GCATC 4 cut(s) 577, 856, 1006, 1135
BmuI ACTGGG 2 cut(s) 871, 1013
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 1 cut(s) 1047
Bpu1102I GCTNAGC 2 cut(s) 555, 1269
BpuEI CTTGAG 4 cut(s) 122, 618, 819, 1373
BsaJI CCNNGG 6 cut(s) 289, 335, 465, 510, 1215, 1365
BsaWI WCCGGW 1 cut(s) 451
Bsc4I CCNNNNNNNGG 7 cut(s) 439, 511, 545, 608, 1040, 1324, 1371
Bse1I ACTGG 5 cut(s) 853, 866, 1008, 1030, 1426
Bse3DI GCAATG 2 cut(s) 1071, 1188
BseAI TCCGGA 1 cut(s) 451
BseBI CCWGG 2 cut(s) 1128, 1217
BseDI CCNNGG 6 cut(s) 289, 335, 465, 510, 1215, 1365
BseGI GGATG 6 cut(s) 349, 460, 496, 568, 871, 1049
BseLI CCNNNNNNNGG 7 cut(s) 439, 511, 545, 608, 1040, 1324, 1371
BseMI GCAATG 2 cut(s) 1071, 1188
BseMII CTCAG 2 cut(s) 569, 1260
BseNI ACTGG 5 cut(s) 853, 866, 1008, 1030, 1426
BseRI GAGGAG 2 cut(s) 239, 1131
BseSI GKGCMC 4 cut(s) 92, 179, 551, 1318
BseXI GCAGC 5 cut(s) 21, 613, 616, 760, 856
BshFI GGCC 8 cut(s) 177, 334, 368, 503, 699, 903, 1316, 1364
BshNI GGYRCC 2 cut(s) 590, 1123
BsiHKCI CYCGRG 1 cut(s) 1086
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 750
BslI CCNNNNNNNGG 7 cut(s) 439, 511, 545, 608, 1040, 1324, 1371
BsmAI GTCTC 1 cut(s) 722
BsmFI GGGAC 1 cut(s) 750
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 8 cut(s) 177, 334, 368, 503, 699, 903, 1316, 1364
BsoBI CYCGRG 1 cut(s) 1086
Bsp120I GGGCCC 2 cut(s) 175, 1314
Bsp1286I GDGCHC 4 cut(s) 92, 179, 551, 1318
Bsp13I TCCGGA 1 cut(s) 451
Bsp143I GATC 3 cut(s) 97, 748, 1030
Bsp1720I GCTNAGC 2 cut(s) 555, 1269
Bsp19I CCATGG 1 cut(s) 1365
BspACI CCGC 2 cut(s) 80, 1412
BspANI GGCC 8 cut(s) 177, 334, 368, 503, 699, 903, 1316, 1364
BspCNI CTCAG 2 cut(s) 568, 1261
BspEI TCCGGA 1 cut(s) 451
BspHI TCATGA 1 cut(s) 970
BspLI GGNNCC 7 cut(s) 177, 178, 526, 592, 1125, 1315, 1316
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 1 cut(s) 1025
BspT107I GGYRCC 2 cut(s) 590, 1123
BsrDI GCAATG 2 cut(s) 1071, 1188
BsrI ACTGG 5 cut(s) 853, 866, 1008, 1030, 1426
BssECI CCNNGG 6 cut(s) 289, 335, 465, 510, 1215, 1365
BssMI GATC 3 cut(s) 97, 748, 1030
BssSI CACGAG 1 cut(s) 1221
BssT1I CCWWGG 4 cut(s) 335, 465, 510, 1365
Bst2BI CACGAG 1 cut(s) 1221
Bst2UI CCWGG 2 cut(s) 1128, 1217
Bst4CI ACNGT 4 cut(s) 587, 816, 1102, 1259
BstAPI GCANNNNNTGC 1 cut(s) 626
BstDEI CTNAG 2 cut(s) 555, 1269
BstDSI CCRYGG 2 cut(s) 289, 1365
BstEII GGTNACC 1 cut(s) 707
BstENI CCTNNNNNAGG 2 cut(s) 509, 606
BstF5I GGATG 6 cut(s) 349, 460, 496, 568, 871, 1049
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 3 cut(s) 100, 751, 1033
BstMAI GTCTC 1 cut(s) 722
BstMBI GATC 3 cut(s) 97, 748, 1030
BstMWI GCNNNNNNNGC 5 cut(s) 183, 565, 626, 779, 878
BstNI CCWGG 2 cut(s) 1128, 1217
BstNSI RCATGY 1 cut(s) 583
BstPI GGTNACC 1 cut(s) 707
BstSCI CCNGG 2 cut(s) 1126, 1215
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 4 cut(s) 92, 179, 551, 1318
BstV1I GCAGC 5 cut(s) 21, 613, 616, 760, 856
BstX2I RGATCY 2 cut(s) 97, 1030
BstYI RGATCY 2 cut(s) 97, 1030
BsuI GTATCC 2 cut(s) 1042, 1204
BsuRI GGCC 8 cut(s) 177, 334, 368, 503, 699, 903, 1316, 1364
BtgI CCRYGG 2 cut(s) 289, 1365
BtrI CACGTC 1 cut(s) 87
BtsCI GGATG 6 cut(s) 349, 460, 496, 568, 871, 1049
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 4 cut(s) 121, 812, 1023, 1207
CaiI CAGNNNCTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 970
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 6 cut(s) 77, 175, 176, 502, 1314, 1315
Csp6I GTAC 1 cut(s) 202
CviAII CATG 6 cut(s) 434, 580, 733, 941, 971, 1366
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 555, 1269
DpnI GATC 3 cut(s) 99, 750, 1032
DpnII GATC 3 cut(s) 97, 748, 1030
DraI TTTAAA 1 cut(s) 919
DriI GACNNNNNGTC 1 cut(s) 75
EaeI YGGCCR 2 cut(s) 366, 697
Eam1105I GACNNNNNGTC 1 cut(s) 75
Eco130I CCWWGG 4 cut(s) 335, 465, 510, 1365
Eco24I GRGCYC 2 cut(s) 179, 1318
Eco47I GGWCC 1 cut(s) 77
Eco57I CTGAAG 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 1086
Eco91I GGTNACC 1 cut(s) 707
EcoNI CCTNNNNNAGG 2 cut(s) 509, 606
EcoO109I RGGNCCY 3 cut(s) 175, 176, 1314
EcoO65I GGTNACC 1 cut(s) 707
EcoRII CCWGG 2 cut(s) 1126, 1215
EcoT14I CCWWGG 4 cut(s) 335, 465, 510, 1365
EcoT22I ATGCAT 2 cut(s) 570, 622
EcoT38I GRGCYC 2 cut(s) 179, 1318
ErhI CCWWGG 4 cut(s) 335, 465, 510, 1365
FaeI CATG 6 cut(s) 437, 583, 736, 944, 974, 1369
FaqI GGGAC 1 cut(s) 750
FatI CATG 6 cut(s) 433, 579, 732, 940, 970, 1365
FauNDI CATATG 1 cut(s) 622
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 5 cut(s) 35, 627, 630, 774, 870
FokI GGATG 6 cut(s) 356, 467, 503, 555, 878, 1056
FriOI GRGCYC 2 cut(s) 179, 1318
Fsp4HI GCNGC 5 cut(s) 35, 627, 630, 774, 870
FspBI CTAG 3 cut(s) 183, 597, 608
GlaI GCGC 1 cut(s) 318
GluI GCNGC 5 cut(s) 35, 627, 630, 774, 870
GsuI CTGGAG 1 cut(s) 1047
HaeIII GGCC 8 cut(s) 177, 334, 368, 503, 699, 903, 1316, 1364
HapII CCGG 1 cut(s) 452
HhaI GCGC 1 cut(s) 319
Hin1II CATG 6 cut(s) 437, 583, 736, 944, 974, 1369
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HindIII AAGCTT 2 cut(s) 407, 1244
HinfI GANTC 6 cut(s) 229, 414, 715, 801, 990, 1227
HpaII CCGG 1 cut(s) 452
HphI GGTGA 3 cut(s) 686, 1349, 1388
Hpy166II GTNNAC 3 cut(s) 1001, 1226, 1379
Hpy188III TCNNGA 7 cut(s) 101, 452, 721, 767, 836, 971, 1352
Hpy8I GTNNAC 3 cut(s) 1001, 1226, 1379
HpyAV CCTTC 4 cut(s) 7, 241, 1354, 1401
HpyCH4III ACNGT 4 cut(s) 587, 816, 1102, 1259
HpyCH4IV ACGT 1 cut(s) 86
HpyF10VI GCNNNNNNNGC 5 cut(s) 183, 565, 626, 779, 878
HpyF3I CTNAG 2 cut(s) 555, 1269
HpySE526I ACGT 1 cut(s) 86
Hsp92II CATG 6 cut(s) 437, 583, 736, 944, 974, 1369
HspAI GCGC 1 cut(s) 317
Kpn2I TCCGGA 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 748
Kzo9I GATC 3 cut(s) 97, 748, 1030
LmnI GCTCC 3 cut(s) 299, 454, 514
Lsp1109I GCAGC 5 cut(s) 21, 613, 616, 760, 856
LweI GCATC 4 cut(s) 577, 856, 1006, 1135
MaeI CTAG 3 cut(s) 183, 597, 608
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 4 cut(s) 67, 655, 707, 956
MalI GATC 3 cut(s) 99, 750, 1032
MboI GATC 3 cut(s) 97, 748, 1030
MboII GAAGA 5 cut(s) 34, 250, 387, 802, 1369
MflI RGATCY 2 cut(s) 97, 1030
MhlI GDGCHC 4 cut(s) 92, 179, 551, 1318
MlsI TGGCCA 1 cut(s) 368
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 4 cut(s) 423, 724, 984, 1221
Mox20I TGGCCA 1 cut(s) 368
Mph1103I ATGCAT 2 cut(s) 570, 622
MroI TCCGGA 1 cut(s) 451
MscI TGGCCA 1 cut(s) 368
MseI TTAA 4 cut(s) 165, 639, 918, 924
MslI CAYNNNNRTG 3 cut(s) 588, 855, 1200
Msp20I TGGCCA 1 cut(s) 368
MspA1I CMGCKG 2 cut(s) 559, 963
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 2 cut(s) 1128, 1217
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 2 cut(s) 1128, 1217
MwoI GCNNNNNNNGC 5 cut(s) 183, 565, 626, 779, 878
NcoI CCATGG 1 cut(s) 1365
NdeI CATATG 1 cut(s) 622
NdeII GATC 3 cut(s) 97, 748, 1030
NlaIII CATG 6 cut(s) 437, 583, 736, 944, 974, 1369
NlaIV GGNNCC 7 cut(s) 177, 178, 526, 592, 1125, 1315, 1316
NmuCI GTSAC 3 cut(s) 67, 655, 956
NsiI ATGCAT 2 cut(s) 570, 622
NspI RCATGY 1 cut(s) 583
OliI CACNNNNGTG 1 cut(s) 588
PaeR7I CTCGAG 1 cut(s) 1086
PagI TCATGA 1 cut(s) 970
PcsI WCGNNNNNNNCGW 1 cut(s) 1228
PctI GAATGC 1 cut(s) 272
PfeI GAWTC 2 cut(s) 229, 801
PflMI CCANNNNNTGG 1 cut(s) 1371
PkrI GCNGC 5 cut(s) 36, 628, 631, 775, 871
PleI GAGTC 4 cut(s) 422, 723, 984, 1221
PpsI GAGTC 4 cut(s) 422, 723, 984, 1221
Psp6I CCWGG 2 cut(s) 1126, 1215
PspEI GGTNACC 1 cut(s) 707
PspGI CCWGG 2 cut(s) 1126, 1215
PspN4I GGNNCC 7 cut(s) 177, 178, 526, 592, 1125, 1315, 1316
PspOMI GGGCCC 2 cut(s) 175, 1314
PspPI GGNCC 6 cut(s) 77, 175, 176, 502, 1314, 1315
PspXI VCTCGAGB 1 cut(s) 1086
PstI CTGCAG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 2 cut(s) 97, 1030
PvuII CAGCTG 2 cut(s) 559, 963
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 3 cut(s) 588, 855, 1200
SaqAI TTAA 4 cut(s) 165, 639, 918, 924
SatI GCNGC 5 cut(s) 35, 627, 630, 774, 870
Sau3AI GATC 3 cut(s) 97, 748, 1030
Sau96I GGNCC 6 cut(s) 77, 175, 176, 502, 1314, 1315
SchI GAGTC 4 cut(s) 423, 724, 984, 1221
ScrFI CCNGG 2 cut(s) 1128, 1217
SduI GDGCHC 4 cut(s) 92, 179, 551, 1318
SfaNI GCATC 4 cut(s) 577, 856, 1006, 1135
SfcI CTRYAG 1 cut(s) 93
Sfr274I CTCGAG 1 cut(s) 1086
SinI GGWCC 1 cut(s) 77
SlaI CTCGAG 1 cut(s) 1086
SmiMI CAYNNNNRTG 3 cut(s) 588, 855, 1200
SmlI CTYRAG 5 cut(s) 101, 633, 834, 1086, 1352
SmoI CTYRAG 5 cut(s) 101, 633, 834, 1086, 1352
SsiI CCGC 2 cut(s) 80, 1412
SspMI CTAG 3 cut(s) 183, 597, 608
StyD4I CCNGG 2 cut(s) 1126, 1215
StyI CCWWGG 4 cut(s) 335, 465, 510, 1365
TaaI ACNGT 4 cut(s) 587, 816, 1102, 1259
TaiI ACGT 1 cut(s) 89
TaqI TCGA 4 cut(s) 417, 535, 1087, 1176
TfiI GAWTC 2 cut(s) 229, 801
Tru1I TTAA 4 cut(s) 165, 639, 918, 924
Tru9I TTAA 4 cut(s) 165, 639, 918, 924
TscAI CASTG 4 cut(s) 121, 819, 1030, 1207
TseFI GTSAC 3 cut(s) 67, 655, 956
TseI GCWGC 5 cut(s) 34, 626, 629, 773, 869
Tsp45I GTSAC 3 cut(s) 67, 655, 956
TspDTI ATGAA 7 cut(s) 20, 26, 260, 388, 395, 1071, 1177
TspGWI ACGGA 3 cut(s) 278, 1171, 1220
TspRI CASTG 4 cut(s) 121, 819, 1030, 1207
Van91I CCANNNNNTGG 1 cut(s) 1371
VpaK11BI GGWCC 1 cut(s) 77
XagI CCTNNNNNAGG 2 cut(s) 509, 606
XapI RAATTY 5 cut(s) 153, 531, 883, 914, 1058
XceI RCATGY 1 cut(s) 583
XcmI CCANNNNNNNNNTGG 1 cut(s) 1325
XhoI CTCGAG 1 cut(s) 1086
XspI CTAG 3 cut(s) 183, 597, 608
Zsp2I ATGCAT 2 cut(s) 570, 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.