RLG00000008967

Xylose isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003254
Physical Location & Seq
Reverse (-)
43949048 .. 43954229
5182 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008967

Sequence Viewer

Length: 1323 bp
ATGGGAACTGGCTATAAAAACACCACTTCCCCACAAGTGTTTGCTTTAGATGATGAAAATGAAGGCAGAGAAGATATTGGTCCCTCTAGCAAGAACCCCCTTGCATACAAATGGTATAATGCAGAGGAGGAGATTCTGGGGAAGAAAATGAAGGATTGGTTGAGGTTTAGTGTTGCATTTTGGCACACATTCCGTGGAACCGGAGCTGACCCATTCGGTGCGCCTACAAAAAATTGGCCTTGGGAGGATGGAACTAACTCTGTTGCTATGGCCAAGAGAAGAAGTGAGAACATCCTCTGTTATTTTTCTATTTATGTGACAGAGGATATTGCTCCAGATGGCAAAACCTTGGAGGAAACTAATAAAAACTTGGATGAGGTGGTGGCCCTTGCCAAGGAGCTTCAGGGAACCAAAATTCGACCTTTGTGGGGCACAGCTCAGCTGTTTATGCATCCTCGCTACATGCATGGTGGTGCCACTAGCCCTGAACTAGGTGTATATGCATATGCTGCTGCTCAAGTTAAGAAAGCTATTGAGGTCACGCATTATTTAGGTGGTGAAAATTATGTGTTTTGGGGTGGCCGTGAGGGTTACCAGAGTCTCCTGAATACCGACATGGGACGAGAGCTTGATCATCTGGCAAGGTTTCTTGAAGCTGCTGTTGCTTACAAGAAGAAAATTGGATTCAATGGAACACTGTTGATTGAACCGAAGCCTCAAGAACCTACTAAACACCAGTATGACTGGGATGCTGCAACAACAGCAAATTTCCTGCGAAAATATGGCCTTATAGGAGAATTTAAACTTAACATTGAGTGCAACCATGCCACTCTATCTGGTCACAGCTGTCATCATGAGCTTGAAACTGCAAGACTCAATGGTTTACTGGGAAACATTGATGCAAACACTGGAGATCCTCAAATTGGATGGGATACAGATGAATTTCTCACAGACATTGCAGAGGCGACTCGAGTTATGCTTACTGTGGTAAAAAATGGAGGATTGGCACCAGGAGGCTTCAACTTTGATGCAAAATTACGGAGGGAAAGCACAGATGTCGAGGATTTGTTCATTGCTCATATCAGTGGAATGGATACCCTTGCCCGTGGACTCCGTAATGTCGCCAAGCTTATTGAGGACGGTTCTCTGGCTGAGCTTGTTCGCAAACGATATGAGAGTTTTGATACAGAGGTTGGGGCCCAGATAGAGGCTGGTAAGGGTGATTTTGAATATCTTGAGAAGAAGGCCATGGAATGGGGTGAACCAAAAGTTCCTTCTGCCAAGCAGGAACTTGCGGAGAGGCATTTCCAGTCTGCTTTGTAG

Protein Analysis

441

Amino Acids

49.19

Weight (kDa)

5.37

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP_endonuc_2 PF01261 125 - 282 5.6e-06 Xylose isomerase-like TIM barrel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 473, 1006
AccB7I CCANNNNNTGG 1 cut(s) 1254
AciI CCGC 1 cut(s) 1295
AclWI GGATC 1 cut(s) 908
AcoI YGGCCR 2 cut(s) 270, 580
AcsI RAATTY 4 cut(s) 414, 766, 797, 941
AcuI CTGAAG 1 cut(s) 386
AfiI CCNNNNNNNGG 6 cut(s) 394, 428, 491, 923, 1207, 1254
AgsI TTSAA 6 cut(s) 653, 688, 707, 863, 1021, 1229
AjnI CCWGG 1 cut(s) 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 1258, 1290
Alw26I GTCTC 1 cut(s) 605
AlwI GGATC 1 cut(s) 908
Ama87I CYCGRG 1 cut(s) 969
AoxI GGCC 7 cut(s) 236, 270, 384, 580, 784, 1197, 1245
ApaI GGGCCC 1 cut(s) 1201
ApeKI GCWGC 4 cut(s) 509, 512, 656, 752
ApoI RAATTY 4 cut(s) 414, 766, 797, 941
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 4 cut(s) 80, 385, 1197, 1198
AsuHPI GGTGA 3 cut(s) 569, 1232, 1271
AvaI CYCGRG 1 cut(s) 969
AvaII GGWCC 1 cut(s) 80
BaeGI GKGCMC 2 cut(s) 434, 1201
BaeI ACNNNNGTAYC 2 cut(s) 1176, 1209
BalI TGGCCA 1 cut(s) 272
BanI GGYRCC 2 cut(s) 473, 1006
BanII GRGCYC 1 cut(s) 1201
BbvI GCAGC 4 cut(s) 496, 499, 643, 739
BccI CCATC 3 cut(s) 242, 332, 921
BceAI ACGGC 1 cut(s) 567
BcgI CGANNNNNNTGC 2 cut(s) 1039, 1073
BciT130I CCWGG 1 cut(s) 1011
BciVI GTATCC 2 cut(s) 925, 1087
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 1 cut(s) 605
BfaI CTAG 3 cut(s) 87, 480, 491
BfuI GTATCC 2 cut(s) 925, 1087
BisI GCNGC 4 cut(s) 510, 513, 657, 753
BlpI GCTNAGC 2 cut(s) 438, 1152
BlsI GCNGC 4 cut(s) 511, 514, 658, 754
Bme1390I CCNGG 1 cut(s) 1011
Bme18I GGWCC 1 cut(s) 80
BmeT110I CYCGRG 1 cut(s) 969
BmgT120I GGNCC 4 cut(s) 80, 385, 1197, 1198
BmiI GGNNCC 7 cut(s) 82, 199, 409, 475, 1008, 1198, 1199
BmrFI CCNGG 1 cut(s) 1011
BmrI ACTGGG 2 cut(s) 754, 896
BmsI GCATC 4 cut(s) 460, 739, 889, 1018
BmuI ACTGGG 2 cut(s) 754, 896
BpmI CTGGAG 2 cut(s) 318, 930
Bpu1102I GCTNAGC 2 cut(s) 438, 1152
BpuEI CTTGAG 3 cut(s) 501, 702, 1256
BsaJI CCNNGG 6 cut(s) 193, 239, 348, 393, 1105, 1248
BsaWI WCCGGW 1 cut(s) 200
Bsc4I CCNNNNNNNGG 6 cut(s) 394, 428, 491, 923, 1207, 1254
Bse1I ACTGG 6 cut(s) 13, 736, 749, 891, 913, 1309
Bse3DI GCAATG 2 cut(s) 954, 1071
BseBI CCWGG 1 cut(s) 1011
BseDI CCNNGG 6 cut(s) 193, 239, 348, 393, 1105, 1248
BseGI GGATG 6 cut(s) 253, 291, 379, 451, 754, 932
BseLI CCNNNNNNNGG 6 cut(s) 394, 428, 491, 923, 1207, 1254
BseMI GCAATG 2 cut(s) 954, 1071
BseMII CTCAG 2 cut(s) 452, 1143
BseNI ACTGG 6 cut(s) 13, 736, 749, 891, 913, 1309
BseRI GAGGAG 2 cut(s) 140, 143
BseSI GKGCMC 2 cut(s) 434, 1201
BseXI GCAGC 4 cut(s) 496, 499, 643, 739
BshFI GGCC 7 cut(s) 238, 272, 386, 582, 786, 1199, 1247
BshNI GGYRCC 2 cut(s) 473, 1006
BsiHKCI CYCGRG 1 cut(s) 969
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 2 cut(s) 66, 633
BslI CCNNNNNNNGG 6 cut(s) 394, 428, 491, 923, 1207, 1254
BsmAI GTCTC 1 cut(s) 605
BsmFI GGGAC 2 cut(s) 66, 633
BsnI GGCC 7 cut(s) 238, 272, 386, 582, 786, 1199, 1247
BsoBI CYCGRG 1 cut(s) 969
Bsp120I GGGCCC 1 cut(s) 1197
Bsp1286I GDGCHC 2 cut(s) 434, 1201
Bsp143I GATC 2 cut(s) 631, 913
Bsp1720I GCTNAGC 2 cut(s) 438, 1152
Bsp19I CCATGG 1 cut(s) 1248
BspACI CCGC 1 cut(s) 1295
BspANI GGCC 7 cut(s) 238, 272, 386, 582, 786, 1199, 1247
BspCNI CTCAG 2 cut(s) 451, 1144
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 7 cut(s) 82, 199, 409, 475, 1008, 1198, 1199
BspPI GGATC 1 cut(s) 908
BspT107I GGYRCC 2 cut(s) 473, 1006
BsrDI GCAATG 2 cut(s) 954, 1071
BsrI ACTGG 6 cut(s) 13, 736, 749, 891, 913, 1309
BssECI CCNNGG 6 cut(s) 193, 239, 348, 393, 1105, 1248
BssMI GATC 2 cut(s) 631, 913
BssT1I CCWWGG 4 cut(s) 239, 348, 393, 1248
Bst2UI CCWGG 1 cut(s) 1011
Bst4CI ACNGT 3 cut(s) 699, 985, 1142
BstAPI GCANNNNNTGC 1 cut(s) 509
BstDEI CTNAG 2 cut(s) 438, 1152
BstDSI CCRYGG 3 cut(s) 193, 1105, 1248
BstEII GGTNACC 1 cut(s) 590
BstENI CCTNNNNNAGG 2 cut(s) 392, 489
BstF5I GGATG 6 cut(s) 253, 291, 379, 451, 754, 932
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 2 cut(s) 634, 916
BstMAI GTCTC 1 cut(s) 605
BstMBI GATC 2 cut(s) 631, 913
BstMWI GCNNNNNNNGC 4 cut(s) 448, 509, 662, 761
BstNI CCWGG 1 cut(s) 1011
BstNSI RCATGY 1 cut(s) 466
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 1 cut(s) 1009
BstSLI GKGCMC 2 cut(s) 434, 1201
BstV1I GCAGC 4 cut(s) 496, 499, 643, 739
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
BsuI GTATCC 2 cut(s) 925, 1087
BsuRI GGCC 7 cut(s) 238, 272, 386, 582, 786, 1199, 1247
BtgI CCRYGG 3 cut(s) 193, 1105, 1248
BtsCI GGATG 6 cut(s) 253, 291, 379, 451, 754, 932
BtsIMutI CAGTG 3 cut(s) 695, 906, 1090
CciI TCATGA 1 cut(s) 853
CfoI GCGC 1 cut(s) 223
Cfr13I GGNCC 4 cut(s) 80, 385, 1197, 1198
CviAII CATG 6 cut(s) 463, 467, 616, 824, 854, 1249
DdeI CTNAG 2 cut(s) 438, 1152
DpnI GATC 2 cut(s) 633, 915
DpnII GATC 2 cut(s) 631, 913
DraI TTTAAA 1 cut(s) 802
EaeI YGGCCR 2 cut(s) 270, 580
Eco130I CCWWGG 4 cut(s) 239, 348, 393, 1248
Eco24I GRGCYC 1 cut(s) 1201
Eco47I GGWCC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 386
Eco88I CYCGRG 1 cut(s) 969
Eco91I GGTNACC 1 cut(s) 590
EcoNI CCTNNNNNAGG 2 cut(s) 392, 489
EcoO109I RGGNCCY 1 cut(s) 1197
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 1009
EcoT14I CCWWGG 4 cut(s) 239, 348, 393, 1248
EcoT22I ATGCAT 3 cut(s) 453, 468, 505
EcoT38I GRGCYC 1 cut(s) 1201
ErhI CCWWGG 4 cut(s) 239, 348, 393, 1248
FaeI CATG 6 cut(s) 466, 470, 619, 827, 857, 1252
FaqI GGGAC 2 cut(s) 66, 633
FatI CATG 6 cut(s) 462, 466, 615, 823, 853, 1248
FauNDI CATATG 1 cut(s) 505
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 4 cut(s) 510, 513, 657, 753
FokI GGATG 6 cut(s) 260, 278, 386, 438, 761, 939
FriOI GRGCYC 1 cut(s) 1201
Fsp4HI GCNGC 4 cut(s) 510, 513, 657, 753
FspBI CTAG 3 cut(s) 87, 480, 491
GlaI GCGC 1 cut(s) 222
GluI GCNGC 4 cut(s) 510, 513, 657, 753
GsuI CTGGAG 2 cut(s) 318, 930
HaeIII GGCC 7 cut(s) 238, 272, 386, 582, 786, 1199, 1247
HapII CCGG 1 cut(s) 201
HhaI GCGC 1 cut(s) 223
Hin1II CATG 6 cut(s) 466, 470, 619, 827, 857, 1252
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HindIII AAGCTT 1 cut(s) 1127
HinfI GANTC 6 cut(s) 133, 598, 684, 873, 967, 1110
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 569, 1232, 1271
Hpy166II GTNNAC 3 cut(s) 884, 1109, 1262
Hpy188III TCNNGA 6 cut(s) 335, 604, 650, 719, 854, 1235
Hpy8I GTNNAC 3 cut(s) 884, 1109, 1262
HpyAV CCTTC 4 cut(s) 56, 145, 1237, 1284
HpyCH4III ACNGT 3 cut(s) 699, 985, 1142
HpyF10VI GCNNNNNNNGC 4 cut(s) 448, 509, 662, 761
HpyF3I CTNAG 2 cut(s) 438, 1152
Hsp92II CATG 6 cut(s) 466, 470, 619, 827, 857, 1252
HspAI GCGC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 2 cut(s) 631, 913
LmnI GCTCC 3 cut(s) 203, 337, 397
Lsp1109I GCAGC 4 cut(s) 496, 499, 643, 739
LweI GCATC 4 cut(s) 460, 739, 889, 1018
MaeI CTAG 3 cut(s) 87, 480, 491
MaeIII GTNAC 4 cut(s) 316, 538, 590, 839
MalI GATC 2 cut(s) 633, 915
MboI GATC 2 cut(s) 631, 913
MboII GAAGA 5 cut(s) 83, 154, 291, 685, 1252
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 2 cut(s) 434, 1201
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 9 cut(s) 232, 414, 562, 678, 766, 797, 921, 941, 1034
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 4 cut(s) 607, 867, 961, 1104
Mox20I TGGCCA 1 cut(s) 272
Mph1103I ATGCAT 3 cut(s) 453, 468, 505
MscI TGGCCA 1 cut(s) 272
MseI TTAA 3 cut(s) 522, 801, 807
MslI CAYNNNNRTG 4 cut(s) 109, 471, 738, 1083
Msp20I TGGCCA 1 cut(s) 272
MspA1I CMGCKG 2 cut(s) 442, 846
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 1011
MvaI CCWGG 1 cut(s) 1011
MwoI GCNNNNNNNGC 4 cut(s) 448, 509, 662, 761
NcoI CCATGG 1 cut(s) 1248
NdeI CATATG 1 cut(s) 505
NdeII GATC 2 cut(s) 631, 913
NlaIII CATG 6 cut(s) 466, 470, 619, 827, 857, 1252
NlaIV GGNNCC 7 cut(s) 82, 199, 409, 475, 1008, 1198, 1199
NmuCI GTSAC 3 cut(s) 316, 538, 839
NsiI ATGCAT 3 cut(s) 453, 468, 505
NspI RCATGY 1 cut(s) 466
PaeR7I CTCGAG 1 cut(s) 969
PagI TCATGA 1 cut(s) 853
PfeI GAWTC 2 cut(s) 133, 684
PflMI CCANNNNNTGG 1 cut(s) 1254
PkrI GCNGC 4 cut(s) 511, 514, 658, 754
PleI GAGTC 4 cut(s) 606, 867, 961, 1104
PpsI GAGTC 4 cut(s) 606, 867, 961, 1104
Psp6I CCWGG 1 cut(s) 1009
PspEI GGTNACC 1 cut(s) 590
PspGI CCWGG 1 cut(s) 1009
PspN4I GGNNCC 7 cut(s) 82, 199, 409, 475, 1008, 1198, 1199
PspOMI GGGCCC 1 cut(s) 1197
PspPI GGNCC 4 cut(s) 80, 385, 1197, 1198
PspXI VCTCGAGB 1 cut(s) 969
PsuI RGATCY 1 cut(s) 913
PvuII CAGCTG 2 cut(s) 442, 846
RseI CAYNNNNRTG 4 cut(s) 109, 471, 738, 1083
SaqAI TTAA 3 cut(s) 522, 801, 807
SatI GCNGC 4 cut(s) 510, 513, 657, 753
Sau3AI GATC 2 cut(s) 631, 913
Sau96I GGNCC 4 cut(s) 80, 385, 1197, 1198
SchI GAGTC 4 cut(s) 607, 867, 961, 1104
ScrFI CCNGG 1 cut(s) 1011
SduI GDGCHC 2 cut(s) 434, 1201
SfaNI GCATC 4 cut(s) 460, 739, 889, 1018
Sfr274I CTCGAG 1 cut(s) 969
SinI GGWCC 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 969
SmiMI CAYNNNNRTG 4 cut(s) 109, 471, 738, 1083
SmlI CTYRAG 4 cut(s) 516, 717, 969, 1235
SmoI CTYRAG 4 cut(s) 516, 717, 969, 1235
Sse9I AATT 9 cut(s) 232, 414, 562, 678, 766, 797, 921, 941, 1034
SsiI CCGC 1 cut(s) 1295
SspMI CTAG 3 cut(s) 87, 480, 491
StyD4I CCNGG 1 cut(s) 1009
StyI CCWWGG 4 cut(s) 239, 348, 393, 1248
TaaI ACNGT 3 cut(s) 699, 985, 1142
TaqI TCGA 3 cut(s) 418, 970, 1059
TasI AATT 9 cut(s) 232, 414, 562, 678, 766, 797, 921, 941, 1034
TfiI GAWTC 2 cut(s) 133, 684
Tru1I TTAA 3 cut(s) 522, 801, 807
Tru9I TTAA 3 cut(s) 522, 801, 807
TscAI CASTG 3 cut(s) 702, 913, 1090
TseFI GTSAC 3 cut(s) 316, 538, 839
TseI GCWGC 4 cut(s) 509, 512, 656, 752
Tsp45I GTSAC 3 cut(s) 316, 538, 839
TspDTI ATGAA 5 cut(s) 69, 75, 164, 954, 1060
TspGWI ACGGA 3 cut(s) 182, 1054, 1103
TspRI CASTG 3 cut(s) 702, 913, 1090
Van91I CCANNNNNTGG 1 cut(s) 1254
VpaK11BI GGWCC 1 cut(s) 80
XagI CCTNNNNNAGG 2 cut(s) 392, 489
XapI RAATTY 4 cut(s) 414, 766, 797, 941
XceI RCATGY 1 cut(s) 466
XcmI CCANNNNNNNNNTGG 1 cut(s) 1208
XhoI CTCGAG 1 cut(s) 969
XspI CTAG 3 cut(s) 87, 480, 491
Zsp2I ATGCAT 3 cut(s) 453, 468, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.