FvH4_4g32520

Xylose isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003254
Physical Location & Seq
Reverse (-)
31170047 .. 31177116
7070 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32520.t2

Sequence Viewer

Length: 1437 bp
ATGAAAATGAAGGCAGAGAAGATATTGCTGCTGCTTGTTTGTGTCAGCTTCTGGATTTTTGGGGTGACTGCTGGTCCCCAGACGTGCCCTGCAGATCTTGAGAGTGATTGCAATGATTCTGGGGAATGGAAAGGGGAATTTTTCCCTGAAATTCCCAAGATTAAATACGAGGGCCCCTCTAGCAAGAACCCCCTTTCATACAAATGGTATAATGCAGAGGAGGAGATTCTAGGAAAGAAAATGAAGGATTGGATGAGGTTTAGTGTTGCATTTTGGCACACATTCCGTGGATCAGGAGCTGATCCATTCGGTGCGCCTACAAAAAATTGGCCTTGGGAGGATGGAACTAACTCTGTGGCTATGGCCAAGAGAAGAATGAAAGCCAACTTTGAGTTCATAAATAAGCTCGGAGTCGACAGGTGGTGTTTCCATGACAGGGATATTGCTCCGGATGGCAAAACTTTGGAGGAAACTAATAAAAACTTGGATGAAGTGGTGGCCCTTGCCAAGGATCTTCAGGGAGCCAAAATTCGACCTTTGTGGGGCACAGCTCAGCTGTTTATGCATCCTCGCTACATGCACGGTGGTGCCACTAGCTCTGAACTAGGTGTGTATGCATATGCTGCTGCTCAAGTTAAGAAAGCTATTGAGGTTACACATTATTTAGGTGGTGAAAATTATGTGTTTTGGGGTGGCCGTGAGGGTTATCAGAGTCTCCTAAATACTGACATGGGACGAGAGCTTGATCATCTGGCAAGGTTTCTTGAAGCTGCTGTTGCTTACAAGAAGAAAATTGGATTTAATGGAACATTATTGATTGAACCAAAGCCTCAAGAACCTACTAAACACCAGTATGACTGGGATGCTGCAACAACAGCAAATTTCCTTCGAAAATATGGTCTTTTAGGAGAATTTAAACTTAACATTGAGTGCAACCATGCCACTCTATCTGGTCACAGCTGTCATCATGAGCTCGAAACTGCAAGACTCAATGGTTTACTGGGAAACATTGATGCAAACACTGGAGATCCTCAAATTGGATGGGATACAGATGAGTTTCTCACAGACATTGCAGAGGCAACTCGAGTTATGCTCACTGTGGTAAAAAATGGAGGACTGGCACCAGGAGGTTTTAACTTTGATGCAAAATTACGGAGAGAAAGCACAGATGTTGAGGATTTGTTCGTTGCTCATATTAGTGGAATGGATACGCTGGCACGTGGACTTCATAATGTTGCCAAGCTTATTGAGGACGGTTCTCTTGCTGAGCTTGTTCGCAAACGATATGAGAGTTTCGATACAGAAGTTGGGGCCCAGATAGAGGCTGGTAAGGGTGATTTTGAATATCTTGAGAAGAAGGCCATGGAATGGGGTGAACCAAAAGTTCCTTCTGCCAAGCAGGAACTTGCGGAGAGACTTTTCCAATTCGCTTTGTAG

Protein Analysis

479

Amino Acids

53.67

Weight (kDa)

5.65

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP_endonuc_2 PF01261 162 - 320 9.9e-06 Xylose isomerase-like TIM barrel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 587, 1120
AccB7I CCANNNNNTGG 1 cut(s) 1368
AccI GTMKAC 1 cut(s) 414
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 1 cut(s) 1409
AclWI GGATC 4 cut(s) 296, 298, 519, 1022
AcoI YGGCCR 2 cut(s) 363, 694
AcsI RAATTY 5 cut(s) 137, 150, 528, 880, 911
AcuI CTGAAG 1 cut(s) 500
AcvI CACGTG 1 cut(s) 1220
AfiI CCNNNNNNNGG 6 cut(s) 436, 508, 542, 1037, 1321, 1368
AgsI TTSAA 3 cut(s) 767, 821, 1343
AhdI GACNNNNNGTC 1 cut(s) 72
AjiI CACGTC 1 cut(s) 84
AjnI CCWGG 1 cut(s) 1123
AjuI GAANNNNNNNTTGG 4 cut(s) 377, 409, 1372, 1404
AleI CACNNNNGTG 1 cut(s) 585
Alw21I GWGCWC 1 cut(s) 975
Alw26I GTCTC 2 cut(s) 719, 1408
AlwI GGATC 4 cut(s) 296, 298, 519, 1022
AlwNI CAGNNNCTG 2 cut(s) 51, 299
Ama87I CYCGRG 1 cut(s) 1083
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 7 cut(s) 172, 329, 363, 498, 694, 1311, 1359
ApaI GGGCCC 2 cut(s) 176, 1315
ApeKI GCWGC 6 cut(s) 28, 31, 623, 626, 770, 866
ApoI RAATTY 5 cut(s) 137, 150, 528, 880, 911
ArsI GACNNNNNNTTYG 2 cut(s) 883, 915
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 6 cut(s) 74, 172, 173, 499, 1311, 1312
AsuHPI GGTGA 4 cut(s) 76, 683, 1346, 1385
AsuII TTCGAA 1 cut(s) 889
AvaI CYCGRG 1 cut(s) 1083
AvaII GGWCC 1 cut(s) 74
BaeGI GKGCMC 4 cut(s) 89, 176, 548, 1315
BaeI ACNNNNGTAYC 2 cut(s) 1290, 1323
BalI TGGCCA 1 cut(s) 365
BanI GGYRCC 2 cut(s) 587, 1120
BanII GRGCYC 3 cut(s) 176, 975, 1315
BbrPI CACGTG 1 cut(s) 1220
Bbv12I GWGCWC 1 cut(s) 975
BbvI GCAGC 6 cut(s) 15, 18, 610, 613, 757, 853
BccI CCATC 3 cut(s) 335, 446, 1035
BceAI ACGGC 1 cut(s) 681
BciT130I CCWGG 1 cut(s) 1125
BciVI GTATCC 2 cut(s) 1039, 1201
BclI TGATCA 1 cut(s) 745
BcoDI GTCTC 2 cut(s) 719, 1408
BfaI CTAG 4 cut(s) 180, 230, 594, 605
BfmI CTRYAG 1 cut(s) 90
BfuI GTATCC 2 cut(s) 1039, 1201
BglII AGATCT 1 cut(s) 94
BisI GCNGC 6 cut(s) 29, 32, 624, 627, 771, 867
BlpI GCTNAGC 2 cut(s) 552, 1266
BlsI GCNGC 6 cut(s) 30, 33, 625, 628, 772, 868
Bme1390I CCNGG 1 cut(s) 1125
Bme18I GGWCC 1 cut(s) 74
BmeRI GACNNNNNGTC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 1083
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 6 cut(s) 74, 172, 173, 499, 1311, 1312
BmiI GGNNCC 8 cut(s) 76, 174, 175, 523, 589, 1122, 1312, 1313
BmrFI CCNGG 1 cut(s) 1125
BmrI ACTGGG 2 cut(s) 868, 1010
BmsI GCATC 4 cut(s) 574, 853, 1003, 1132
BmuI ACTGGG 2 cut(s) 868, 1010
BplI GAGNNNNNCTC 4 cut(s) 161, 193, 1077, 1109
BpmI CTGGAG 1 cut(s) 1044
Bpu1102I GCTNAGC 2 cut(s) 552, 1266
Bpu14I TTCGAA 1 cut(s) 889
BpuEI CTTGAG 4 cut(s) 119, 615, 816, 1370
BsaAI YACGTR 1 cut(s) 1220
BsaJI CCNNGG 4 cut(s) 286, 332, 507, 1362
BsaWI WCCGGW 1 cut(s) 448
Bsc4I CCNNNNNNNGG 6 cut(s) 436, 508, 542, 1037, 1321, 1368
Bse1I ACTGG 5 cut(s) 850, 863, 1005, 1027, 1122
Bse3DI GCAATG 2 cut(s) 118, 1068
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 1125
BseDI CCNNGG 4 cut(s) 286, 332, 507, 1362
BseGI GGATG 7 cut(s) 258, 346, 457, 493, 565, 868, 1046
BseLI CCNNNNNNNGG 6 cut(s) 436, 508, 542, 1037, 1321, 1368
BseMI GCAATG 2 cut(s) 118, 1068
BseMII CTCAG 2 cut(s) 566, 1257
BseNI ACTGG 5 cut(s) 850, 863, 1005, 1027, 1122
BseRI GAGGAG 2 cut(s) 233, 236
BseSI GKGCMC 4 cut(s) 89, 176, 548, 1315
BseXI GCAGC 6 cut(s) 15, 18, 610, 613, 757, 853
BshFI GGCC 7 cut(s) 174, 331, 365, 500, 696, 1313, 1361
BshNI GGYRCC 2 cut(s) 587, 1120
BsiHKAI GWGCWC 1 cut(s) 975
BsiHKCI CYCGRG 1 cut(s) 1083
BsiSI CCGG 1 cut(s) 449
BslFI GGGAC 2 cut(s) 60, 747
BslI CCNNNNNNNGG 6 cut(s) 436, 508, 542, 1037, 1321, 1368
BsmAI GTCTC 2 cut(s) 719, 1408
BsmFI GGGAC 2 cut(s) 60, 747
BsnI GGCC 7 cut(s) 174, 331, 365, 500, 696, 1313, 1361
BsoBI CYCGRG 1 cut(s) 1083
Bsp119I TTCGAA 1 cut(s) 889
Bsp120I GGGCCC 2 cut(s) 172, 1311
Bsp1286I GDGCHC 5 cut(s) 89, 176, 548, 975, 1315
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 6 cut(s) 94, 290, 301, 511, 745, 1027
Bsp1720I GCTNAGC 2 cut(s) 552, 1266
Bsp19I CCATGG 1 cut(s) 1362
BspACI CCGC 1 cut(s) 1409
BspANI GGCC 7 cut(s) 174, 331, 365, 500, 696, 1313, 1361
BspCNI CTCAG 2 cut(s) 565, 1258
BspEI TCCGGA 1 cut(s) 448
BspHI TCATGA 1 cut(s) 967
BspLI GGNNCC 8 cut(s) 76, 174, 175, 523, 589, 1122, 1312, 1313
BspMAI CTGCAG 1 cut(s) 94
BspPI GGATC 4 cut(s) 296, 298, 519, 1022
BspT104I TTCGAA 1 cut(s) 889
BspT107I GGYRCC 2 cut(s) 587, 1120
BsrDI GCAATG 2 cut(s) 118, 1068
BsrI ACTGG 5 cut(s) 850, 863, 1005, 1027, 1122
BssECI CCNNGG 4 cut(s) 286, 332, 507, 1362
BssMI GATC 6 cut(s) 94, 290, 301, 511, 745, 1027
BssT1I CCWWGG 3 cut(s) 332, 507, 1362
Bst2UI CCWGG 1 cut(s) 1125
Bst4CI ACNGT 3 cut(s) 584, 1099, 1256
BstAPI GCANNNNNTGC 1 cut(s) 623
BstBAI YACGTR 1 cut(s) 1220
BstBI TTCGAA 1 cut(s) 889
BstC8I GCNNGC 1 cut(s) 1215
BstDEI CTNAG 2 cut(s) 552, 1266
BstDSI CCRYGG 2 cut(s) 286, 1362
BstENI CCTNNNNNAGG 1 cut(s) 506
BstF5I GGATG 7 cut(s) 258, 346, 457, 493, 565, 868, 1046
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 6 cut(s) 97, 293, 304, 514, 748, 1030
BstMAI GTCTC 2 cut(s) 719, 1408
BstMBI GATC 6 cut(s) 94, 290, 301, 511, 745, 1027
BstMWI GCNNNNNNNGC 5 cut(s) 180, 562, 623, 776, 875
BstNI CCWGG 1 cut(s) 1125
BstNSI RCATGY 1 cut(s) 580
BstSCI CCNGG 1 cut(s) 1123
BstSFI CTRYAG 1 cut(s) 90
BstSLI GKGCMC 4 cut(s) 89, 176, 548, 1315
BstV1I GCAGC 6 cut(s) 15, 18, 610, 613, 757, 853
BstX2I RGATCY 3 cut(s) 94, 511, 1027
BstYI RGATCY 3 cut(s) 94, 511, 1027
BsuI GTATCC 2 cut(s) 1039, 1201
BsuRI GGCC 7 cut(s) 174, 331, 365, 500, 696, 1313, 1361
BtgI CCRYGG 2 cut(s) 286, 1362
BtrI CACGTC 1 cut(s) 84
BtsCI GGATG 7 cut(s) 258, 346, 457, 493, 565, 868, 1046
BtsIMutI CAGTG 2 cut(s) 1020, 1095
Cac8I GCNNGC 1 cut(s) 1215
CaiI CAGNNNCTG 2 cut(s) 51, 299
CciI TCATGA 1 cut(s) 967
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 6 cut(s) 74, 172, 173, 499, 1311, 1312
CviAII CATG 6 cut(s) 431, 577, 730, 938, 968, 1363
DdeI CTNAG 2 cut(s) 552, 1266
DpnI GATC 6 cut(s) 96, 292, 303, 513, 747, 1029
DpnII GATC 6 cut(s) 94, 290, 301, 511, 745, 1027
DraI TTTAAA 1 cut(s) 916
DriI GACNNNNNGTC 1 cut(s) 72
EaeI YGGCCR 2 cut(s) 363, 694
Eam1105I GACNNNNNGTC 1 cut(s) 72
Ecl136II GAGCTC 1 cut(s) 973
Eco130I CCWWGG 3 cut(s) 332, 507, 1362
Eco24I GRGCYC 3 cut(s) 176, 975, 1315
Eco47I GGWCC 1 cut(s) 74
Eco53kI GAGCTC 1 cut(s) 973
Eco57I CTGAAG 1 cut(s) 500
Eco72I CACGTG 1 cut(s) 1220
Eco88I CYCGRG 1 cut(s) 1083
EcoICRI GAGCTC 1 cut(s) 973
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoO109I RGGNCCY 3 cut(s) 172, 173, 1311
EcoRII CCWGG 1 cut(s) 1123
EcoT14I CCWWGG 3 cut(s) 332, 507, 1362
EcoT22I ATGCAT 2 cut(s) 567, 619
EcoT38I GRGCYC 3 cut(s) 176, 975, 1315
ErhI CCWWGG 3 cut(s) 332, 507, 1362
FaeI CATG 6 cut(s) 434, 580, 733, 941, 971, 1366
FaqI GGGAC 2 cut(s) 60, 747
FatI CATG 6 cut(s) 430, 576, 729, 937, 967, 1362
FauNDI CATATG 1 cut(s) 619
FbaI TGATCA 1 cut(s) 745
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 6 cut(s) 29, 32, 624, 627, 771, 867
FokI GGATG 7 cut(s) 265, 353, 464, 500, 552, 875, 1053
FriOI GRGCYC 3 cut(s) 176, 975, 1315
Fsp4HI GCNGC 6 cut(s) 29, 32, 624, 627, 771, 867
FspBI CTAG 4 cut(s) 180, 230, 594, 605
GlaI GCGC 1 cut(s) 315
GluI GCNGC 6 cut(s) 29, 32, 624, 627, 771, 867
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 7 cut(s) 174, 331, 365, 500, 696, 1313, 1361
HapII CCGG 1 cut(s) 449
HhaI GCGC 1 cut(s) 316
Hin1II CATG 6 cut(s) 434, 580, 733, 941, 971, 1366
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HindIII AAGCTT 1 cut(s) 1241
HinfI GANTC 5 cut(s) 116, 226, 411, 712, 987
HpaII CCGG 1 cut(s) 449
HphI GGTGA 4 cut(s) 76, 683, 1346, 1385
Hpy166II GTNNAC 4 cut(s) 415, 998, 1223, 1376
Hpy188I TCNGA 3 cut(s) 410, 601, 711
Hpy188III TCNNGA 8 cut(s) 52, 98, 294, 449, 764, 833, 968, 1349
Hpy8I GTNNAC 4 cut(s) 415, 998, 1223, 1376
HpyAV CCTTC 5 cut(s) 4, 238, 896, 1351, 1398
HpyCH4III ACNGT 3 cut(s) 584, 1099, 1256
HpyCH4IV ACGT 2 cut(s) 83, 1219
HpyF10VI GCNNNNNNNGC 5 cut(s) 180, 562, 623, 776, 875
HpyF3I CTNAG 2 cut(s) 552, 1266
HpySE526I ACGT 2 cut(s) 83, 1219
Hsp92II CATG 6 cut(s) 434, 580, 733, 941, 971, 1366
HspAI GCGC 1 cut(s) 314
Kpn2I TCCGGA 1 cut(s) 448
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 6 cut(s) 94, 290, 301, 511, 745, 1027
LmnI GCTCC 3 cut(s) 296, 451, 521
Lsp1109I GCAGC 6 cut(s) 15, 18, 610, 613, 757, 853
LweI GCATC 4 cut(s) 574, 853, 1003, 1132
MaeI CTAG 4 cut(s) 180, 230, 594, 605
MaeII ACGT 2 cut(s) 83, 1219
MaeIII GTNAC 3 cut(s) 64, 652, 953
MalI GATC 6 cut(s) 96, 292, 303, 513, 747, 1029
MboI GATC 6 cut(s) 94, 290, 301, 511, 745, 1027
MboII GAAGA 5 cut(s) 31, 384, 506, 799, 1366
MflI RGATCY 3 cut(s) 94, 511, 1027
MhlI GDGCHC 5 cut(s) 89, 176, 548, 975, 1315
MlsI TGGCCA 1 cut(s) 365
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 3 cut(s) 420, 721, 981
Mox20I TGGCCA 1 cut(s) 365
Mph1103I ATGCAT 2 cut(s) 567, 619
MroI TCCGGA 1 cut(s) 448
MscI TGGCCA 1 cut(s) 365
MseI TTAA 6 cut(s) 162, 636, 801, 915, 921, 1134
MslI CAYNNNNRTG 4 cut(s) 202, 585, 852, 1197
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 2 cut(s) 556, 960
MspI CCGG 1 cut(s) 449
MspR9I CCNGG 1 cut(s) 1125
MvaI CCWGG 1 cut(s) 1125
MwoI GCNNNNNNNGC 5 cut(s) 180, 562, 623, 776, 875
NcoI CCATGG 1 cut(s) 1362
NdeI CATATG 1 cut(s) 619
NdeII GATC 6 cut(s) 94, 290, 301, 511, 745, 1027
NlaIII CATG 6 cut(s) 434, 580, 733, 941, 971, 1366
NlaIV GGNNCC 8 cut(s) 76, 174, 175, 523, 589, 1122, 1312, 1313
NmuCI GTSAC 2 cut(s) 64, 953
NsiI ATGCAT 2 cut(s) 567, 619
NspI RCATGY 1 cut(s) 580
NspV TTCGAA 1 cut(s) 889
OliI CACNNNNGTG 1 cut(s) 585
PaeR7I CTCGAG 1 cut(s) 1083
PagI TCATGA 1 cut(s) 967
PfeI GAWTC 2 cut(s) 116, 226
PflMI CCANNNNNTGG 1 cut(s) 1368
PkrI GCNGC 6 cut(s) 30, 33, 625, 628, 772, 868
PleI GAGTC 3 cut(s) 419, 720, 981
PmaCI CACGTG 1 cut(s) 1220
PmlI CACGTG 1 cut(s) 1220
PpsI GAGTC 3 cut(s) 419, 720, 981
Ppu21I YACGTR 1 cut(s) 1220
Psp124BI GAGCTC 1 cut(s) 975
Psp6I CCWGG 1 cut(s) 1123
PspCI CACGTG 1 cut(s) 1220
PspGI CCWGG 1 cut(s) 1123
PspN4I GGNNCC 8 cut(s) 76, 174, 175, 523, 589, 1122, 1312, 1313
PspOMI GGGCCC 2 cut(s) 172, 1311
PspPI GGNCC 6 cut(s) 74, 172, 173, 499, 1311, 1312
PspXI VCTCGAGB 1 cut(s) 1083
PstI CTGCAG 1 cut(s) 94
PstNI CAGNNNCTG 2 cut(s) 51, 299
PsuI RGATCY 3 cut(s) 94, 511, 1027
PvuII CAGCTG 2 cut(s) 556, 960
RseI CAYNNNNRTG 4 cut(s) 202, 585, 852, 1197
SacI GAGCTC 1 cut(s) 975
SalI GTCGAC 1 cut(s) 413
SaqAI TTAA 6 cut(s) 162, 636, 801, 915, 921, 1134
SatI GCNGC 6 cut(s) 29, 32, 624, 627, 771, 867
Sau3AI GATC 6 cut(s) 94, 290, 301, 511, 745, 1027
Sau96I GGNCC 6 cut(s) 74, 172, 173, 499, 1311, 1312
SchI GAGTC 3 cut(s) 420, 721, 981
ScrFI CCNGG 1 cut(s) 1125
SduI GDGCHC 5 cut(s) 89, 176, 548, 975, 1315
SfaNI GCATC 4 cut(s) 574, 853, 1003, 1132
SfcI CTRYAG 1 cut(s) 90
Sfr274I CTCGAG 1 cut(s) 1083
SfuI TTCGAA 1 cut(s) 889
SinI GGWCC 1 cut(s) 74
SlaI CTCGAG 1 cut(s) 1083
SmiMI CAYNNNNRTG 4 cut(s) 202, 585, 852, 1197
SmlI CTYRAG 5 cut(s) 98, 630, 831, 1083, 1349
SmoI CTYRAG 5 cut(s) 98, 630, 831, 1083, 1349
SsiI CCGC 1 cut(s) 1409
SspMI CTAG 4 cut(s) 180, 230, 594, 605
SstI GAGCTC 1 cut(s) 975
StyD4I CCNGG 1 cut(s) 1123
StyI CCWWGG 3 cut(s) 332, 507, 1362
TaaI ACNGT 3 cut(s) 584, 1099, 1256
TaiI ACGT 2 cut(s) 86, 1222
TaqI TCGA 6 cut(s) 414, 532, 889, 975, 1084, 1296
TfiI GAWTC 2 cut(s) 116, 226
Tru1I TTAA 6 cut(s) 162, 636, 801, 915, 921, 1134
Tru9I TTAA 6 cut(s) 162, 636, 801, 915, 921, 1134
TscAI CASTG 2 cut(s) 1027, 1102
TseFI GTSAC 2 cut(s) 64, 953
TseI GCWGC 6 cut(s) 28, 31, 623, 626, 770, 866
Tsp45I GTSAC 2 cut(s) 64, 953
TspDTI ATGAA 8 cut(s) 17, 23, 186, 257, 385, 392, 504, 1217
TspGWI ACGGA 2 cut(s) 275, 1168
TspRI CASTG 2 cut(s) 1027, 1102
Van91I CCANNNNNTGG 1 cut(s) 1368
VpaK11BI GGWCC 1 cut(s) 74
XagI CCTNNNNNAGG 1 cut(s) 506
XapI RAATTY 5 cut(s) 137, 150, 528, 880, 911
XceI RCATGY 1 cut(s) 580
XcmI CCANNNNNNNNNTGG 1 cut(s) 1322
XhoI CTCGAG 1 cut(s) 1083
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 4 cut(s) 180, 230, 594, 605
Zsp2I ATGCAT 2 cut(s) 567, 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.