AT5G57960

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003824
Physical Location & Seq
Reverse (-)
23462704 .. 23466678
3975 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57960.2

Sequence Viewer

Length: 1623 bp
ATGAGCTCGTTTTACCTTTCTTCTTCTCCAATTTTCAAACTTCAGTGGCACGCAAACCATAAACCTAAACCCAATCGTGCAATAGTTTCTTTTCCACCTTCTCGTCTTCATGCCAACTGCTATTCGTGGAGATTATCTTGTAACCTTGCTCAACATGGTATTGAGTTGGAGGAAACTGTGGAGGAAGATGAAATCCTTCAAGTTCTCGACTTACCAACAGAGGAGACAAATCTCGACAACGAAACGATTGCTTCTCCATCTAAAATGTTGAGGAAGAAAAAAGGAGATGAAGAGAGCTTGGACGATAGATTCAAGCTCAGAAATGGAAAGGAGATTTTCGAAGAGAAGGCATATCTAGTGGGCGTGGAAAGAAAGGGTGATGGAGAATGCTTGTTTAATATTGAGGAATCTCTTGAGGAGCTAGAACAACTTGCGGATACTGCTGGGCTTGCAGTGGTTGGTTCTACTTATCAAAAGCTAGCTTCCCCAAATCCTAGAACATACATTGGATCAGGAAAGGTCGCAGAGATAAAAAGTGCAATTAATGCTTTGGACGTTGAGACTGTGATATTTGACGATGAACTTTCACCAGGGCAATTGCGTAACCTGGAAAAGGCCTTTGGTGGGGATGTTCGAGTTTGTGACCGCACCGCCCTTATCTTGGACATTTTCAACCAAAGGGCCGCAACTCATGAAGCAGCCTTGCAGGTTGCTCTAGCACAAATGGAGTACCAGTTGCCACGATTAACTAGAATGTGGACTCATCTTGAGCGTCAATCTGGTGGCCAAGTTAAGGGTATGGGAGAAAAACAAATTGAAGTTGATAAGCGAATCTTGCGTACTCAAATTGGAGTTCTCAAAAAGGAGTTGGAATCTGTAAGAAAGCATCGGAAGCAGTATAGAAGCAGGCGTGTTGCTATACCTGTACCTGTTGTATCCTTGGTCGGTTACACAAACGCTGGAAAGAGTACACTATTGAACCAATTGACTGGTGCTAATGTTCTCGCTGAAAATCGTTTGTTTGCAACTCTTGATCCAACTACGAGAAGGGTTCAGATGCAAAACGGGAAGGAATTTCTTCTCACAGATACTGTTGGTTTTATCCAAAAGTTACCAACCACTCTGGTTGCTGCTTTCAGAGCAACACTTGAAGAAATAGCAGAGTCAAGCCTTTTGGTGCATGTTGTTGACATCAGCCACCCACTGGCAGAGCAACAAATAGAAGCTGTGGAGAAGGTTATGTCTGAACTCGACGTTTCATCAATTCCAAAATTGGTCGTGTGGAATAAGGTTGATAGAGTGGATGATCCTCAAAAGGTCAAGCTGGAAGCAGAGGAAACTGGGGATACAATTTGTATATCTGCTCTGACTGGAGAAGGACTAGACGACTTCTGCAATGCTGTTCATGAGAAGCTCAAGGATTCAATGGTTTGGGTTGAAGCCCTTTTGCCATTTGATAAAGGGGACCTTCTAAGCACCATACACAAGGTTGGAATGGTGAAAGAAACTGAATATACAGAGAATGGGACACTTATCAGGGCACACGTTCCGCTACGTTTTGCACAGCTGCTTAAACCTATGAGACACTTGGTCAAAGATACTTCAATAAGCCAAAGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

540

Amino Acids

60.53

Weight (kDa)

5.76

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-bdg_N PF13167 3 - 70 4.2e-23 GTP-binding GTPase N-terminal
GTP-bdg_M PF16360 73 - 150 2.3e-27 GTP-binding GTPase Middle Region
MMR_HSR1 PF01926 158 - 276 3.6e-22 50S ribosome-binding GTPase
FeoB_N PF02421 158 - 307 1.5e-06 Ferrous iron transport protein B
HflX_C PF19275 287 - 357 6.9e-09 HflX C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 697
AccB7I CCANNNNNTGG 1 cut(s) 1204
AciI CCGC 5 cut(s) 434, 646, 651, 684, 1550
AclWI GGATC 3 cut(s) 517, 1028, 1301
AcoI YGGCCR 1 cut(s) 784
AcsI RAATTY 1 cut(s) 1073
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 4 cut(s) 731, 841, 927, 970
AfiI CCNNNNNNNGG 5 cut(s) 613, 624, 661, 793, 1204
AflIII ACRYGT 1 cut(s) 1543
AhdI GACNNNNNGTC 1 cut(s) 1589
AjnI CCWGG 2 cut(s) 589, 606
AjuI GAANNNNNNNTTGG 2 cut(s) 603, 635
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 3 cut(s) 218, 554, 1576
AlwI GGATC 3 cut(s) 517, 1028, 1301
AlwNI CAGNNNCTG 1 cut(s) 1091
AoxI GGCC 3 cut(s) 615, 681, 784
ApeKI GCWGC 3 cut(s) 698, 1130, 1567
ApoI RAATTY 1 cut(s) 1073
AseI ATTAAT 1 cut(s) 543
Asp700I GAANNNNTTC 2 cut(s) 195, 1077
AspS9I GGNCC 2 cut(s) 681, 1465
AsuHPI GGTGA 3 cut(s) 389, 579, 1510
AsuII TTCGAA 1 cut(s) 339
AsuNHI GCTAGC 1 cut(s) 478
AvaII GGWCC 1 cut(s) 1465
BaeGI GKGCMC 1 cut(s) 1543
BalI TGGCCA 1 cut(s) 786
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 3 cut(s) 710, 1117, 1554
BccI CCATC 2 cut(s) 265, 374
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 2 cut(s) 591, 608
BciVI GTATCC 3 cut(s) 430, 946, 1339
BcoDI GTCTC 3 cut(s) 218, 554, 1576
BfaI CTAG 7 cut(s) 356, 422, 479, 495, 716, 750, 1382
BfuAI ACCTGC 1 cut(s) 697
BfuI GTATCC 3 cut(s) 430, 946, 1339
BisI GCNGC 4 cut(s) 684, 699, 1131, 1568
BlsI GCNGC 4 cut(s) 685, 700, 1132, 1569
Bme1390I CCNGG 2 cut(s) 591, 608
Bme18I GGWCC 1 cut(s) 1465
BmeRI GACNNNNNGTC 1 cut(s) 1589
BmgT120I GGNCC 2 cut(s) 681, 1465
BmiI GGNNCC 1 cut(s) 1466
BmrFI CCNGG 2 cut(s) 591, 608
BmrI ACTGGG 1 cut(s) 1350
BmsI GCATC 2 cut(s) 895, 1047
BmtI GCTAGC 1 cut(s) 482
BmuI ACTGGG 1 cut(s) 1350
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 1392
Bpu14I TTCGAA 1 cut(s) 339
BpuEI CTTGAG 3 cut(s) 434, 788, 1400
BsaJI CCNNGG 2 cut(s) 590, 939
BsaXI ACNNNNNCTCC 2 cut(s) 719, 749
Bsc4I CCNNNNNNNGG 5 cut(s) 613, 624, 661, 793, 1204
Bse1I ACTGG 5 cut(s) 733, 994, 1209, 1345, 1375
Bse3DI GCAATG 1 cut(s) 1402
BseBI CCWGG 2 cut(s) 591, 608
BseDI CCNNGG 2 cut(s) 590, 939
BseGI GGATG 2 cut(s) 634, 1309
BseLI CCNNNNNNNGG 5 cut(s) 613, 624, 661, 793, 1204
BseMI GCAATG 1 cut(s) 1402
BseMII CTCAG 1 cut(s) 331
BseNI ACTGG 5 cut(s) 733, 994, 1209, 1345, 1375
BseRI GAGGAG 2 cut(s) 236, 431
BseSI GKGCMC 1 cut(s) 1543
BseXI GCAGC 3 cut(s) 710, 1117, 1554
BseYI CCCAGC 1 cut(s) 443
BshFI GGCC 3 cut(s) 617, 683, 786
BsiHKAI GWGCWC 1 cut(s) 8
BslFI GGGAC 2 cut(s) 1478, 1540
BslI CCNNNNNNNGG 5 cut(s) 613, 624, 661, 793, 1204
BsmAI GTCTC 3 cut(s) 218, 554, 1576
BsmFI GGGAC 2 cut(s) 1478, 1540
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 3 cut(s) 617, 683, 786
Bsp119I TTCGAA 1 cut(s) 339
Bsp1286I GDGCHC 2 cut(s) 8, 1543
Bsp143I GATC 3 cut(s) 509, 1033, 1306
BspACI CCGC 5 cut(s) 434, 646, 651, 684, 1550
BspANI GGCC 3 cut(s) 617, 683, 786
BspCNI CTCAG 1 cut(s) 330
BspHI TCATGA 2 cut(s) 691, 1405
BspLI GGNNCC 1 cut(s) 1466
BspMI ACCTGC 1 cut(s) 697
BspOI GCTAGC 1 cut(s) 482
BspPI GGATC 3 cut(s) 517, 1028, 1301
BspT104I TTCGAA 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 1402
BsrI ACTGG 5 cut(s) 733, 994, 1209, 1345, 1375
BssECI CCNNGG 2 cut(s) 590, 939
BssMI GATC 3 cut(s) 509, 1033, 1306
BssT1I CCWWGG 1 cut(s) 939
Bst2UI CCWGG 2 cut(s) 591, 608
Bst4CI ACNGT 3 cut(s) 178, 565, 1093
Bst6I CTCTTC 2 cut(s) 285, 336
BstAPI GCANNNNNTGC 1 cut(s) 545
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 4 cut(s) 51, 450, 480, 908
BstDEI CTNAG 2 cut(s) 317, 1472
BstENI CCTNNNNNAGG 1 cut(s) 611
BstF5I GGATG 2 cut(s) 634, 1309
BstKTI GATC 3 cut(s) 512, 1036, 1309
BstMAI GTCTC 3 cut(s) 218, 554, 1576
BstMBI GATC 3 cut(s) 509, 1033, 1306
BstMWI GCNNNNNNNGC 6 cut(s) 440, 449, 545, 835, 892, 1139
BstNI CCWGG 2 cut(s) 591, 608
BstNSI RCATGY 1 cut(s) 1184
BstSCI CCNGG 2 cut(s) 589, 606
BstSLI GKGCMC 1 cut(s) 1543
BstV1I GCAGC 3 cut(s) 710, 1117, 1554
BstV2I GAAGAC 1 cut(s) 98
BstXI CCANNNNNNTGG 1 cut(s) 989
BsuI GTATCC 3 cut(s) 430, 946, 1339
BsuRI GGCC 3 cut(s) 617, 683, 786
BtsCI GGATG 2 cut(s) 634, 1309
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 3 cut(s) 50, 459, 1202
BveI ACCTGC 1 cut(s) 697
Cac8I GCNNGC 4 cut(s) 51, 450, 480, 908
CaiI CAGNNNCTG 1 cut(s) 1091
CciI TCATGA 2 cut(s) 691, 1405
Cfr13I GGNCC 2 cut(s) 681, 1465
CseI GACGC 1 cut(s) 761
Csp6I GTAC 4 cut(s) 730, 840, 926, 969
CspCI CAANNNNNGTGG 2 cut(s) 1108, 1143
CviAII CATG 5 cut(s) 110, 155, 692, 1181, 1406
CviQI GTAC 4 cut(s) 730, 840, 926, 969
DdeI CTNAG 2 cut(s) 317, 1472
DpnI GATC 3 cut(s) 511, 1035, 1308
DpnII GATC 3 cut(s) 509, 1033, 1306
DriI GACNNNNNGTC 1 cut(s) 1589
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 2 cut(s) 285, 336
Eam1105I GACNNNNNGTC 1 cut(s) 1589
EarI CTCTTC 2 cut(s) 285, 336
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 1 cut(s) 939
Eco147I AGGCCT 1 cut(s) 617
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 1465
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 611
EcoO109I RGGNCCY 1 cut(s) 1465
EcoRII CCWGG 2 cut(s) 589, 606
EcoT14I CCWWGG 1 cut(s) 939
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 1 cut(s) 939
FaeI CATG 5 cut(s) 113, 158, 695, 1184, 1409
FalI AAGNNNNNCTT 4 cut(s) 818, 850, 1452, 1484
FaqI GGGAC 2 cut(s) 1478, 1540
FatI CATG 5 cut(s) 109, 154, 691, 1180, 1405
Fnu4HI GCNGC 4 cut(s) 684, 699, 1131, 1568
FokI GGATG 2 cut(s) 641, 1316
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 4 cut(s) 684, 699, 1131, 1568
FspBI CTAG 7 cut(s) 356, 422, 479, 495, 716, 750, 1382
GluI GCNGC 4 cut(s) 684, 699, 1131, 1568
GsaI CCCAGC 1 cut(s) 447
GsuI CTGGAG 1 cut(s) 1392
HaeIII GGCC 3 cut(s) 617, 683, 786
HgaI GACGC 1 cut(s) 761
Hin1II CATG 5 cut(s) 113, 158, 695, 1184, 1409
HincII GTYRAC 1 cut(s) 1189
HindII GTYRAC 1 cut(s) 1189
HinfI GANTC 7 cut(s) 309, 407, 760, 831, 872, 1163, 1421
HphI GGTGA 3 cut(s) 389, 579, 1510
Hpy166II GTNNAC 3 cut(s) 759, 971, 1189
Hpy188I TCNGA 6 cut(s) 320, 891, 1056, 1139, 1246, 1368
Hpy188III TCNNGA 8 cut(s) 206, 233, 413, 513, 692, 767, 1031, 1406
Hpy8I GTNNAC 3 cut(s) 759, 971, 1189
Hpy99I CGWCG 1 cut(s) 1256
HpyAV CCTTC 8 cut(s) 108, 206, 340, 1041, 1063, 1228, 1370, 1478
HpyCH4III ACNGT 3 cut(s) 178, 565, 1093
HpyCH4IV ACGT 4 cut(s) 555, 1254, 1545, 1555
HpyCH4V TGCA 9 cut(s) 80, 452, 539, 706, 1025, 1060, 1180, 1395, 1562
HpyF10VI GCNNNNNNNGC 6 cut(s) 440, 449, 545, 835, 892, 1139
HpyF3I CTNAG 2 cut(s) 317, 1472
HpySE526I ACGT 4 cut(s) 555, 1254, 1545, 1555
Hsp92II CATG 5 cut(s) 113, 158, 695, 1184, 1409
Kzo9I GATC 3 cut(s) 509, 1033, 1306
LmnI GCTCC 1 cut(s) 418
Lsp1109I GCAGC 3 cut(s) 710, 1117, 1554
LweI GCATC 2 cut(s) 895, 1047
MaeI CTAG 7 cut(s) 356, 422, 479, 495, 716, 750, 1382
MaeII ACGT 4 cut(s) 555, 1254, 1545, 1555
MaeIII GTNAC 5 cut(s) 140, 602, 641, 947, 1110
MalI GATC 3 cut(s) 511, 1035, 1308
MboI GATC 3 cut(s) 509, 1033, 1306
MboII GAAGA 9 cut(s) 12, 15, 98, 197, 286, 302, 353, 1070, 1163
MfeI CAATTG 2 cut(s) 596, 983
MhlI GDGCHC 2 cut(s) 8, 1543
MlsI TGGCCA 1 cut(s) 786
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 2 cut(s) 754, 1172
MmeI TCCRAC 4 cut(s) 147, 849, 1061, 1471
MnlI CCTC 9 cut(s) 163, 175, 214, 264, 397, 409, 1320, 1327, 1610
Mox20I TGGCCA 1 cut(s) 786
MroXI GAANNNNTTC 2 cut(s) 195, 1077
MscI TGGCCA 1 cut(s) 786
MseI TTAA 5 cut(s) 396, 543, 746, 792, 1572
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 1567
MspR9I CCNGG 2 cut(s) 591, 608
MunI CAATTG 2 cut(s) 596, 983
Mva1269I GAATGC 1 cut(s) 392
MvaI CCWGG 2 cut(s) 591, 608
MwoI GCNNNNNNNGC 6 cut(s) 440, 449, 545, 835, 892, 1139
NdeII GATC 3 cut(s) 509, 1033, 1306
NheI GCTAGC 1 cut(s) 478
NlaIII CATG 5 cut(s) 113, 158, 695, 1184, 1409
NlaIV GGNNCC 1 cut(s) 1466
NmuCI GTSAC 1 cut(s) 641
NspI RCATGY 1 cut(s) 1184
NspV TTCGAA 1 cut(s) 339
PagI TCATGA 2 cut(s) 691, 1405
PceI AGGCCT 1 cut(s) 617
PctI GAATGC 1 cut(s) 392
PdmI GAANNNNTTC 2 cut(s) 195, 1077
PfeI GAWTC 5 cut(s) 309, 407, 831, 872, 1421
PflMI CCANNNNNTGG 1 cut(s) 1204
PkrI GCNGC 4 cut(s) 685, 700, 1132, 1569
PleI GAGTC 2 cut(s) 754, 1171
PpsI GAGTC 2 cut(s) 754, 1171
PpuMI RGGWCCY 1 cut(s) 1465
PshBI ATTAAT 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 1465
Psp6I CCWGG 2 cut(s) 589, 606
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 2 cut(s) 589, 606
PspN4I GGNNCC 1 cut(s) 1466
PspPI GGNCC 2 cut(s) 681, 1465
PspPPI RGGWCCY 1 cut(s) 1465
PstNI CAGNNNCTG 1 cut(s) 1091
PvuII CAGCTG 1 cut(s) 1567
RsaI GTAC 4 cut(s) 731, 841, 927, 970
RsaNI GTAC 4 cut(s) 730, 840, 926, 969
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 5 cut(s) 396, 543, 746, 792, 1572
SatI GCNGC 4 cut(s) 684, 699, 1131, 1568
Sau3AI GATC 3 cut(s) 509, 1033, 1306
Sau96I GGNCC 2 cut(s) 681, 1465
SchI GAGTC 2 cut(s) 754, 1172
ScrFI CCNGG 2 cut(s) 591, 608
SduI GDGCHC 2 cut(s) 8, 1543
SfaNI GCATC 2 cut(s) 895, 1047
SfuI TTCGAA 1 cut(s) 339
SinI GGWCC 1 cut(s) 1465
SmlI CTYRAG 3 cut(s) 413, 767, 1415
SmoI CTYRAG 3 cut(s) 413, 767, 1415
SseBI AGGCCT 1 cut(s) 617
SsiI CCGC 5 cut(s) 434, 646, 651, 684, 1550
SspI AATATT 1 cut(s) 400
SspMI CTAG 7 cut(s) 356, 422, 479, 495, 716, 750, 1382
SstI GAGCTC 1 cut(s) 8
StuI AGGCCT 1 cut(s) 617
StyD4I CCNGG 2 cut(s) 589, 606
StyI CCWWGG 1 cut(s) 939
TaaI ACNGT 3 cut(s) 178, 565, 1093
TaiI ACGT 4 cut(s) 558, 1257, 1548, 1558
TaqI TCGA 5 cut(s) 207, 234, 339, 634, 1251
TatI WGTACW 1 cut(s) 968
TauI GCSGC 1 cut(s) 686
TfiI GAWTC 5 cut(s) 309, 407, 831, 872, 1421
Tru1I TTAA 5 cut(s) 396, 543, 746, 792, 1572
Tru9I TTAA 5 cut(s) 396, 543, 746, 792, 1572
TscAI CASTG 3 cut(s) 50, 459, 1209
TseFI GTSAC 1 cut(s) 641
TseI GCWGC 3 cut(s) 698, 1130, 1567
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 7 cut(s) 98, 204, 303, 594, 708, 1248, 1394
TspRI CASTG 3 cut(s) 50, 459, 1209
Van91I CCANNNNNTGG 1 cut(s) 1204
VpaK11BI GGWCC 1 cut(s) 1465
VspI ATTAAT 1 cut(s) 543
XagI CCTNNNNNAGG 1 cut(s) 611
XapI RAATTY 1 cut(s) 1073
XceI RCATGY 1 cut(s) 1184
XmnI GAANNNNTTC 2 cut(s) 195, 1077
XspI CTAG 7 cut(s) 356, 422, 479, 495, 716, 750, 1382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.