Rw6G036430

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003824
Physical Location & Seq
Reverse (-)
60902495 .. 60905777
3283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G036430.1

Sequence Viewer

Length: 1113 bp
ATGAGCGCCAGTTTGTGTTGCTCTCTGATTCGTTCCTCACCTCTGCTTCCCGAACCCTATCTTTCATGGCCTCTCGCCAAACCTAACCACCACCGCCAGATTCAACCGCCTCTCACGCTCAGAAACCATGCCCGTGCTTCTGCGACAGTCGTCGCAAGAGCTGTCGGCGTCGGCGTTGTGTCTCCGGACGACTTGTCGTTTCAGGAACCGCCGGTGATTGAGACCGAGGAAGAAGGAAATGGAGAGGCTCAGGGAGTCGTCGATGTAGTGGCGGAGAGTGAATCAGAGGACAAGGGTGTGCGTCCGGCGACGAGGTACAAGAAGAAGAAGGAGGATGAGGACGGGTTAGAGAATAGGTTCAAGCTGAGGAACGGAAAGGAGATTTTTGAAGAAAAAGCCTACATTGTGGGTGTTGAGCACAAACGTAATTCGGCTGATCTATTTGGTGTAGAGGAGTCACTCAAGGAATTGACCCAGCTAGCTGATACTGCTGGACTAATGGTTGTTGGTTCCACTTATCAGAAACTAGTGTCTCCAAATTCACGGACATACATTGGATCTGGCAAGGTTGCAGAAATCAAGAGTGCAATTCATGCATTGGGTGTTGAGACCGTGATATTTGATGATGAGCTCTCAGCAGGGCAATTACGCAATTTGGAAAAGGCTTTTGGTGGGGATGTTAGAGTCTGTGACCGCACTGCTCTCATTCTTGATATTTTTAACCAGCGGGCGGCAACACATGAAGCAGCTCTACAGGTTACATTGGCACAGATGGAATACCAGCTACCTCGACTAACAAAAATGTGGACTCATCTTGAGCGTCAATCAGGAGGTCAAGTGAAGGGTATGGGTGAGAAACAAATTGAAGTGGATAAGCGTATCTTACGTACTCAAATTGGTGTTCTCAAGAAGGAGCTAGAATCTGTTAGAAAGCATAGAAAGCAGTACCGGAATCAGCGGCTTTCAGTACCAGTCCCAGTAGTATCCTTGGTTGGGTACACCAATGCTGGAAAGAGTACTCTCTTAAATCAATTGACTGGAGCCAATGTGCTTGCCGAGGATCAATTATTTGCAACACTAGATCCAACCACAAGAAGGGTTCAGGTAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

370

Amino Acids

40.87

Weight (kDa)

7.72

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-bdg_N PF13167 151 - 240 2.2e-30 GTP-binding GTPase N-terminal
GTP-bdg_M PF16360 243 - 320 3.2e-27 GTP-binding GTPase Middle Region
MMR_HSR1 PF01926 328 - 368 1.8e-10 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 8 cut(s) 94, 107, 209, 272, 694, 727, 731, 958
AclWI GGATC 3 cut(s) 565, 1068, 1076
AcsI RAATTY 1 cut(s) 538
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 6 cut(s) 317, 889, 947, 969, 998, 1018
AfiI CCNNNNNNNGG 3 cut(s) 730, 993, 1095
AgsI TTSAA 4 cut(s) 104, 361, 389, 866
AhdI GACNNNNNGTC 1 cut(s) 193
AhlI ACTAGT 1 cut(s) 526
AjuI GAANNNNNNNTTGG 2 cut(s) 651, 683
AluBI AGCT 8 cut(s) 161, 364, 478, 482, 631, 749, 784, 916
AluI AGCT 8 cut(s) 161, 364, 478, 482, 631, 749, 784, 916
Alw21I GWGCWC 2 cut(s) 420, 633
Alw26I GTCTC 4 cut(s) 186, 215, 537, 602
AlwI GGATC 3 cut(s) 565, 1068, 1076
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 1 cut(s) 746
ApoI RAATTY 1 cut(s) 538
Asp700I GAANNNNTTC 1 cut(s) 356
AspLEI GCGC 1 cut(s) 8
AsuHPI GGTGA 3 cut(s) 30, 226, 863
AsuNHI GCTAGC 1 cut(s) 478
BanII GRGCYC 1 cut(s) 633
BarI GAAGNNNNNNTAC 2 cut(s) 735, 767
Bbv12I GWGCWC 2 cut(s) 420, 633
BbvCI CCTCAGC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 758
BccI CCATC 1 cut(s) 766
BciVI GTATCC 1 cut(s) 994
BcoDI GTCTC 4 cut(s) 186, 215, 537, 602
BcuI ACTAGT 1 cut(s) 526
BfaI CTAG 4 cut(s) 479, 527, 917, 1079
BfmI CTRYAG 1 cut(s) 752
BfoI RGCGCY 1 cut(s) 9
BfuI GTATCC 1 cut(s) 994
BisI GCNGC 3 cut(s) 732, 747, 959
BlsI GCNGC 3 cut(s) 733, 748, 960
BmcAI AGTACT 1 cut(s) 1018
BmeRI GACNNNNNGTC 1 cut(s) 193
BmiI GGNNCC 3 cut(s) 207, 511, 1042
BmrI ACTGGG 1 cut(s) 971
BmtI GCTAGC 1 cut(s) 482
BmuI ACTGGG 1 cut(s) 971
BoxI GACNNNNGTC 1 cut(s) 149
BpmI CTGGAG 1 cut(s) 1059
Bpu10I CCTNAGC 2 cut(s) 249, 365
BpuEI CTTGAG 3 cut(s) 446, 836, 890
BsaAI YACGTR 1 cut(s) 887
BsaHI GRCGYC 1 cut(s) 168
BsaI GGTCTC 2 cut(s) 215, 602
BsaJI CCNNGG 3 cut(s) 225, 987, 1056
BsaWI WCCGGW 2 cut(s) 184, 948
BsaXI ACNNNNNCTCC 4 cut(s) 822, 852, 1032, 1062
Bsc4I CCNNNNNNNGG 3 cut(s) 730, 993, 1095
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 4 cut(s) 9, 971, 977, 1042
BseAI TCCGGA 1 cut(s) 184
BseDI CCNNGG 3 cut(s) 225, 987, 1056
BseGI GGATG 2 cut(s) 340, 682
BseLI CCNNNNNNNGG 3 cut(s) 730, 993, 1095
BseMII CTCAG 4 cut(s) 133, 263, 356, 648
BseNI ACTGG 4 cut(s) 9, 971, 977, 1042
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 1 cut(s) 758
BseYI CCCAGC 1 cut(s) 474
BshFI GGCC 1 cut(s) 70
BsiHKAI GWGCWC 2 cut(s) 420, 633
BsiSI CCGG 4 cut(s) 185, 212, 305, 949
BslFI GGGAC 1 cut(s) 959
BslI CCNNNNNNNGG 3 cut(s) 730, 993, 1095
BsmAI GTCTC 4 cut(s) 186, 215, 537, 602
BsmFI GGGAC 1 cut(s) 959
BsnI GGCC 1 cut(s) 70
Bso31I GGTCTC 2 cut(s) 215, 602
Bsp1286I GDGCHC 2 cut(s) 420, 633
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 4 cut(s) 436, 557, 1060, 1081
BspACI CCGC 8 cut(s) 94, 107, 209, 272, 694, 727, 731, 958
BspANI GGCC 1 cut(s) 70
BspCNI CTCAG 4 cut(s) 132, 262, 357, 647
BspEI TCCGGA 1 cut(s) 184
BspLI GGNNCC 3 cut(s) 207, 511, 1042
BspOI GCTAGC 1 cut(s) 482
BspPI GGATC 3 cut(s) 565, 1068, 1076
BspTNI GGTCTC 2 cut(s) 215, 602
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 4 cut(s) 9, 971, 977, 1042
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 3 cut(s) 225, 987, 1056
BssMI GATC 4 cut(s) 436, 557, 1060, 1081
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 987
Bst4CI ACNGT 2 cut(s) 148, 613
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 593
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 3 cut(s) 480, 729, 1053
BstDEI CTNAG 4 cut(s) 119, 249, 365, 634
BstF5I GGATG 2 cut(s) 340, 682
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 4 cut(s) 439, 560, 1063, 1084
BstMAI GTCTC 4 cut(s) 186, 215, 537, 602
BstMBI GATC 4 cut(s) 436, 557, 1060, 1081
BstMWI GCNNNNNNNGC 4 cut(s) 115, 488, 593, 940
BstPAI GACNNNNGTC 1 cut(s) 149
BstSFI CTRYAG 1 cut(s) 752
BstSNI TACGTA 1 cut(s) 887
BstV1I GCAGC 1 cut(s) 758
BstX2I RGATCY 2 cut(s) 557, 1081
BstYI RGATCY 2 cut(s) 557, 1081
BsuI GTATCC 1 cut(s) 994
BsuRI GGCC 1 cut(s) 70
BtsCI GGATG 2 cut(s) 340, 682
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 1 cut(s) 696
Cac8I GCNNGC 3 cut(s) 480, 729, 1053
CfoI GCGC 1 cut(s) 8
Cfr10I RCCGGY 1 cut(s) 211
CseI GACGC 3 cut(s) 157, 290, 809
Csp6I GTAC 6 cut(s) 316, 888, 946, 968, 997, 1017
CviAII CATG 4 cut(s) 66, 128, 593, 740
CviQI GTAC 6 cut(s) 316, 888, 946, 968, 997, 1017
DdeI CTNAG 4 cut(s) 119, 249, 365, 634
DpnI GATC 4 cut(s) 438, 559, 1062, 1083
DpnII GATC 4 cut(s) 436, 557, 1060, 1081
DriI GACNNNNNGTC 1 cut(s) 193
Eam1105I GACNNNNNGTC 1 cut(s) 193
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 631
Eco105I TACGTA 1 cut(s) 887
Eco130I CCWWGG 1 cut(s) 987
Eco24I GRGCYC 1 cut(s) 633
Eco31I GGTCTC 2 cut(s) 215, 602
Eco53kI GAGCTC 1 cut(s) 631
EcoICRI GAGCTC 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 987
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 633
ErhI CCWWGG 1 cut(s) 987
FaeI CATG 4 cut(s) 69, 131, 596, 743
FaiI YATR 7 cut(s) 67, 129, 550, 594, 741, 848, 936
FalI AAGNNNNNCTT 2 cut(s) 866, 898
FaqI GGGAC 1 cut(s) 959
FatI CATG 4 cut(s) 65, 127, 592, 739
FauI CCCGC 1 cut(s) 720
Fnu4HI GCNGC 3 cut(s) 732, 747, 959
FokI GGATG 2 cut(s) 347, 689
FriOI GRGCYC 1 cut(s) 633
Fsp4HI GCNGC 3 cut(s) 732, 747, 959
FspBI CTAG 4 cut(s) 479, 527, 917, 1079
GlaI GCGC 1 cut(s) 7
GluI GCNGC 3 cut(s) 732, 747, 959
GsaI CCCAGC 1 cut(s) 478
GsuI CTGGAG 1 cut(s) 1059
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 1 cut(s) 70
HapII CCGG 4 cut(s) 185, 212, 305, 949
HgaI GACGC 3 cut(s) 157, 290, 809
HhaI GCGC 1 cut(s) 8
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 4 cut(s) 69, 131, 596, 743
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HinfI GANTC 9 cut(s) 28, 100, 255, 281, 455, 684, 808, 920, 952
HpaII CCGG 4 cut(s) 185, 212, 305, 949
HphI GGTGA 3 cut(s) 30, 226, 863
Hpy166II GTNNAC 2 cut(s) 807, 999
Hpy188I TCNGA 4 cut(s) 27, 122, 286, 522
Hpy188III TCNNGA 8 cut(s) 50, 185, 203, 580, 710, 815, 828, 907
Hpy8I GTNNAC 2 cut(s) 807, 999
Hpy99I CGWCG 4 cut(s) 155, 173, 263, 313
HpyAV CCTTC 5 cut(s) 227, 322, 835, 904, 1089
HpyCH4III ACNGT 2 cut(s) 148, 613
HpyCH4IV ACGT 2 cut(s) 424, 886
HpyCH4V TGCA 4 cut(s) 572, 587, 596, 1073
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 488, 593, 940
HpyF3I CTNAG 4 cut(s) 119, 249, 365, 634
HpySE526I ACGT 2 cut(s) 424, 886
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 4 cut(s) 69, 131, 596, 743
HspAI GCGC 1 cut(s) 6
Kpn2I TCCGGA 1 cut(s) 184
Kzo9I GATC 4 cut(s) 436, 557, 1060, 1081
LmnI GCTCC 2 cut(s) 913, 1040
Lsp1109I GCAGC 1 cut(s) 758
MaeI CTAG 4 cut(s) 479, 527, 917, 1079
MaeII ACGT 2 cut(s) 424, 886
MaeIII GTNAC 3 cut(s) 456, 689, 757
MalI GATC 4 cut(s) 438, 559, 1062, 1083
MboI GATC 4 cut(s) 436, 557, 1060, 1081
MboII GAAGA 4 cut(s) 242, 334, 337, 401
MfeI CAATTG 1 cut(s) 1031
MflI RGATCY 2 cut(s) 557, 1081
MhlI GDGCHC 2 cut(s) 420, 633
MlyI GAGTC 4 cut(s) 264, 464, 693, 802
MmeI TCCRAC 1 cut(s) 1109
Mph1103I ATGCAT 1 cut(s) 598
MroI TCCGGA 1 cut(s) 184
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 2 cut(s) 720, 1025
MslI CAYNNNNRTG 1 cut(s) 132
MspA1I CMGCKG 2 cut(s) 727, 958
MspI CCGG 4 cut(s) 185, 212, 305, 949
MunI CAATTG 1 cut(s) 1031
MwoI GCNNNNNNNGC 4 cut(s) 115, 488, 593, 940
NdeII GATC 4 cut(s) 436, 557, 1060, 1081
NheI GCTAGC 1 cut(s) 478
NlaIII CATG 4 cut(s) 69, 131, 596, 743
NlaIV GGNNCC 3 cut(s) 207, 511, 1042
NmeAIII GCCGAG 1 cut(s) 1081
NmuCI GTSAC 2 cut(s) 456, 689
NsiI ATGCAT 1 cut(s) 598
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 5 cut(s) 28, 100, 281, 920, 952
PkrI GCNGC 3 cut(s) 733, 748, 960
PleI GAGTC 4 cut(s) 263, 463, 692, 802
PpsI GAGTC 4 cut(s) 263, 463, 692, 802
Ppu21I YACGTR 1 cut(s) 887
PshAI GACNNNNGTC 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 633
PspFI CCCAGC 1 cut(s) 474
PspN4I GGNNCC 3 cut(s) 207, 511, 1042
PsuI RGATCY 2 cut(s) 557, 1081
RsaI GTAC 6 cut(s) 317, 889, 947, 969, 998, 1018
RsaNI GTAC 6 cut(s) 316, 888, 946, 968, 997, 1017
RseI CAYNNNNRTG 1 cut(s) 132
SacI GAGCTC 1 cut(s) 633
SaqAI TTAA 2 cut(s) 720, 1025
SatI GCNGC 3 cut(s) 732, 747, 959
Sau3AI GATC 4 cut(s) 436, 557, 1060, 1081
ScaI AGTACT 1 cut(s) 1018
SchI GAGTC 4 cut(s) 264, 464, 693, 802
SduI GDGCHC 2 cut(s) 420, 633
SfcI CTRYAG 1 cut(s) 752
SgrAI CRCCGGYG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 132
SmlI CTYRAG 3 cut(s) 461, 815, 905
SmoI CTYRAG 3 cut(s) 461, 815, 905
SnaBI TACGTA 1 cut(s) 887
SpeI ACTAGT 1 cut(s) 526
SsiI CCGC 8 cut(s) 94, 107, 209, 272, 694, 727, 731, 958
SspMI CTAG 4 cut(s) 479, 527, 917, 1079
SstI GAGCTC 1 cut(s) 633
StyI CCWWGG 1 cut(s) 987
TaaI ACNGT 2 cut(s) 148, 613
TaiI ACGT 2 cut(s) 427, 889
TaqI TCGA 2 cut(s) 261, 790
TaqII GACCGA 1 cut(s) 239
TatI WGTACW 1 cut(s) 1016
TauI GCSGC 2 cut(s) 734, 961
TfiI GAWTC 5 cut(s) 28, 100, 281, 920, 952
Tru1I TTAA 2 cut(s) 720, 1025
Tru9I TTAA 2 cut(s) 720, 1025
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 2 cut(s) 456, 689
TseI GCWGC 1 cut(s) 746
Tsp45I GTSAC 2 cut(s) 456, 689
TspDTI ATGAA 3 cut(s) 54, 581, 756
TspGWI ACGGA 2 cut(s) 387, 559
TspRI CASTG 1 cut(s) 703
XapI RAATTY 1 cut(s) 538
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 4 cut(s) 479, 527, 917, 1079
ZrmI AGTACT 1 cut(s) 1018
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.