Rroxscaffold_7G00166480

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003824
Physical Location & Seq
Forward (+)
7927861 .. 7932959
5099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00166480.1

Sequence Viewer

Length: 1653 bp
ATGAGCGCCAGTTTGTGTTGCTCTCTGATTCATTCCTCACCTCTGCTTCCCGAACCCTATCTTTCATGGCCTCTCGCCGCCAAACCTAACCACCAACGCCAGATTCAACCGCCTCTCACTTTCAGAAACCATGCCCGTACTTCTGCGAGAGTCGTCGCAAGAGCTGGCGGCGTCGGCGTTGTGTCTCCGGACGACTTGTCGTTTCAGGAACCGGCGGTGATTGAGACCGAGGAAGAAGGAAATGGAGAGGCTCAGGGAGTCGTCGATGGAGTGGCGGAGAGTGAATCAGAGGATAAGGGTGTGCGTCCGGCGACGAGGTACAAGAAGAAGAAGGAGGACGAGGACGGGTTAGAGAATAGGTTCAAGCTGAGGAACGGAAAGGAGATTTTTGAAGAAAAAGCCTACATTGTGGGTGTTGAGCACAAACGTAATTCGGCTGATCTATTTGGTGTAGAGGAGTCACTCAAGGAATTGACCCAGCTAGCTGATACTGCTGGACTAATGGTTGTTGGTTCCACATATCAGAAACTAGTGTCTCCAAATTCACGGACATACATTGGATCTGGCAAGGTTGCAGAAATCAAGAGTGCAATTCATGCATTGGGTGTTGAGACCGTGATATTTGATGATGAGCTCTCAGCAGGGCAATTACGCAATTTGGAAAAGGCTTTTGGTGGGGATGTTAGAGTGTGTGACCGCACTGCTCTCATTCTTGATATTTTTAACCAGCGGGCGGCAACACATGAAGCAGCTCTACAGGTTACATTGGCACAGATGGAATACCAGCTACCTCGACTAACAAAAATGTGGACTCATCTTGAGCGTCAATCAGGAGGTCAAGTGAAGGGTATGGGTGAGAAACAAATTGAAGTGGATAAGCGTATCTTACGTACTCAAATTGGTGTTCTCAAGAAGGAGCTAGAATCTGTTAGAAAGCATAGAAAGCAGTACCGGAATCAGCGGCTTTCAGTACCAGTCCCAGTAGTATCCTTGGTTGGGTACACCAATGCTGGAAAGAGTACTCTCTTAAATCAATTGACTGGAGCCAATGTGCTTGCCGAGGATCAATTATTTGCAACACTAGATCCAACCACGAGAAGGGTTCAGATGAAGAACGGGAAAGAATTTCTTCTCACAGATACTGTTGGTTTCATTCAGAAGTTACCGACTACAGTGGTTGCTGCCTTCAGAGCAACTCTTGAGGAGATATCAGAGTCAGCATTACTGGTGCATGTGGTTGATATCAGTCATCCACTGGCTGAGCAACAGATAGAAGCTGTGGACAGAGTTTTGTCAGAACTAGATGTGTCTTCGATACCAAGGTTGATGGTGTGGAACAAGGTTGATAAGGTGAGCAATCCTGACAGCATCAGAACAGAAGCAGAAAAGAGAGATGATGTTGTTTGCATATCTGCTCTGAGTGGCCAGGGCTTAAATGATTTCTGCAGTGCGATCCAGGAAAAATTGAAGGACTCTATGGTATGGGTGGAAGCCTTGATCCCATTTGAAAAAGGGGAGCTTCTTAGTACCATACATAAGGTTGGAATGGTGGAGAGAACAGAATATACCGAGGACGGTACACTAATCAAGGCACACGTACCTCTACGGTTTGCAAGGCTACTAACACCATTGAGGCAACTATGCATATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

550

Amino Acids

60.93

Weight (kDa)

6.14

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-bdg_N PF13167 152 - 241 4.2e-30 GTP-binding GTPase N-terminal
GTP-bdg_M PF16360 244 - 321 5.9e-27 GTP-binding GTPase Middle Region
MMR_HSR1 PF01926 329 - 447 5e-22 50S ribosome-binding GTPase
HflX_C PF19275 457 - 528 1.3e-08 HflX C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 187
AciI CCGC 9 cut(s) 78, 110, 168, 215, 275, 697, 730, 734, 961
AclWI GGATC 5 cut(s) 568, 1071, 1079, 1447, 1492
AcoI YGGCCR 1 cut(s) 1423
AcsI RAATTY 2 cut(s) 541, 1124
AcuI CTGAAG 1 cut(s) 1171
AcyI GRCGYC 1 cut(s) 171
AfiI CCNNNNNNNGG 3 cut(s) 733, 996, 1098
AflIII ACRYGT 1 cut(s) 1594
AgsI TTSAA 6 cut(s) 107, 364, 392, 869, 1468, 1508
AhdI GACNNNNNGTC 1 cut(s) 196
AhlI ACTAGT 1 cut(s) 529
AjnI CCWGG 2 cut(s) 1425, 1455
AjuI GAANNNNNNNTTGG 4 cut(s) 87, 119, 654, 686
Alw21I GWGCWC 2 cut(s) 423, 636
Alw26I GTCTC 4 cut(s) 189, 218, 540, 605
AlwI GGATC 5 cut(s) 568, 1071, 1079, 1447, 1492
AlwNI CAGNNNCTG 1 cut(s) 1142
Aor13HI TCCGGA 1 cut(s) 187
AoxI GGCC 2 cut(s) 68, 1423
ApeKI GCWGC 2 cut(s) 749, 1181
ApoI RAATTY 2 cut(s) 541, 1124
Asp700I GAANNNNTTC 2 cut(s) 359, 1128
AspLEI GCGC 1 cut(s) 8
AsuHPI GGTGA 4 cut(s) 30, 229, 866, 1363
AsuNHI GCTAGC 1 cut(s) 481
BaeI ACNNNNGTAYC 2 cut(s) 1307, 1340
BalI TGGCCA 1 cut(s) 1425
BanII GRGCYC 1 cut(s) 636
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
BauI CACGAG 1 cut(s) 1093
BbsI GAAGAC 1 cut(s) 1302
Bbv12I GWGCWC 2 cut(s) 423, 636
BbvCI CCTCAGC 1 cut(s) 368
BbvI GCAGC 2 cut(s) 761, 1168
BccI CCATC 3 cut(s) 260, 769, 1321
BciT130I CCWGG 2 cut(s) 1427, 1457
BciVI GTATCC 1 cut(s) 997
BcoDI GTCTC 4 cut(s) 189, 218, 540, 605
BcuI ACTAGT 1 cut(s) 529
BfaI CTAG 5 cut(s) 482, 530, 920, 1082, 1301
BfmI CTRYAG 3 cut(s) 755, 1170, 1444
BfoI RGCGCY 1 cut(s) 9
BfuI GTATCC 1 cut(s) 997
BisI GCNGC 6 cut(s) 78, 169, 735, 750, 962, 1182
BlpI GCTNAGC 1 cut(s) 1260
BlsI GCNGC 6 cut(s) 79, 170, 736, 751, 963, 1183
BmcAI AGTACT 1 cut(s) 1021
Bme1390I CCNGG 2 cut(s) 1427, 1457
BmeRI GACNNNNNGTC 1 cut(s) 196
BmiI GGNNCC 3 cut(s) 210, 514, 1045
BmrFI CCNGG 2 cut(s) 1427, 1457
BmrI ACTGGG 1 cut(s) 974
BmsI GCATC 1 cut(s) 1377
BmtI GCTAGC 1 cut(s) 485
BmuI ACTGGG 1 cut(s) 974
BpiI GAAGAC 1 cut(s) 1302
BpmI CTGGAG 1 cut(s) 1062
Bpu10I CCTNAGC 2 cut(s) 252, 368
Bpu1102I GCTNAGC 1 cut(s) 1260
BpuEI CTTGAG 4 cut(s) 449, 839, 893, 1220
BsaAI YACGTR 2 cut(s) 890, 1597
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 2 cut(s) 218, 605
BsaJI CCNNGG 6 cut(s) 228, 990, 1059, 1319, 1426, 1569
BsaWI WCCGGW 2 cut(s) 187, 951
BsaXI ACNNNNNCTCC 4 cut(s) 825, 855, 1035, 1065
Bsc4I CCNNNNNNNGG 3 cut(s) 733, 996, 1098
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 6 cut(s) 9, 974, 980, 1045, 1230, 1260
BseAI TCCGGA 1 cut(s) 187
BseBI CCWGG 2 cut(s) 1427, 1457
BseDI CCNNGG 6 cut(s) 228, 990, 1059, 1319, 1426, 1569
BseGI GGATG 2 cut(s) 685, 1249
BseLI CCNNNNNNNGG 3 cut(s) 733, 996, 1098
BseMII CTCAG 5 cut(s) 266, 359, 651, 1251, 1409
BseNI ACTGG 6 cut(s) 9, 974, 980, 1045, 1230, 1260
BseRI GAGGAG 2 cut(s) 470, 1217
BseXI GCAGC 2 cut(s) 761, 1168
BseYI CCCAGC 1 cut(s) 477
BshFI GGCC 2 cut(s) 70, 1425
BsiHKAI GWGCWC 2 cut(s) 423, 636
BsiSI CCGG 4 cut(s) 188, 212, 308, 952
BslFI GGGAC 1 cut(s) 962
BslI CCNNNNNNNGG 3 cut(s) 733, 996, 1098
BsmAI GTCTC 4 cut(s) 189, 218, 540, 605
BsmFI GGGAC 1 cut(s) 962
BsnI GGCC 2 cut(s) 70, 1425
Bso31I GGTCTC 2 cut(s) 218, 605
Bsp1286I GDGCHC 2 cut(s) 423, 636
Bsp13I TCCGGA 1 cut(s) 187
Bsp143I GATC 6 cut(s) 439, 560, 1063, 1084, 1452, 1497
Bsp1720I GCTNAGC 1 cut(s) 1260
BspACI CCGC 9 cut(s) 78, 110, 168, 215, 275, 697, 730, 734, 961
BspANI GGCC 2 cut(s) 70, 1425
BspCNI CTCAG 5 cut(s) 265, 360, 650, 1252, 1410
BspEI TCCGGA 1 cut(s) 187
BspHI TCATGA 1 cut(s) 1649
BspLI GGNNCC 3 cut(s) 210, 514, 1045
BspMAI CTGCAG 1 cut(s) 1448
BspOI GCTAGC 1 cut(s) 485
BspPI GGATC 5 cut(s) 568, 1071, 1079, 1447, 1492
BspTNI GGTCTC 2 cut(s) 218, 605
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 6 cut(s) 9, 974, 980, 1045, 1230, 1260
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 6 cut(s) 228, 990, 1059, 1319, 1426, 1569
BssMI GATC 6 cut(s) 439, 560, 1063, 1084, 1452, 1497
BssNI GRCGYC 1 cut(s) 171
BssSI CACGAG 1 cut(s) 1093
BssT1I CCWWGG 2 cut(s) 990, 1319
Bst2BI CACGAG 1 cut(s) 1093
Bst2UI CCWGG 2 cut(s) 1427, 1457
Bst4CI ACNGT 5 cut(s) 616, 1144, 1174, 1577, 1608
BstACI GRCGYC 1 cut(s) 171
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBAI YACGTR 2 cut(s) 890, 1597
BstC8I GCNNGC 4 cut(s) 166, 483, 732, 1056
BstDEI CTNAG 6 cut(s) 252, 368, 637, 1260, 1418, 1523
BstF5I GGATG 2 cut(s) 685, 1249
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 6 cut(s) 442, 563, 1066, 1087, 1455, 1500
BstMAI GTCTC 4 cut(s) 189, 218, 540, 605
BstMBI GATC 6 cut(s) 439, 560, 1063, 1084, 1452, 1497
BstMWI GCNNNNNNNGC 5 cut(s) 174, 491, 596, 943, 1190
BstNI CCWGG 2 cut(s) 1427, 1457
BstNSI RCATGY 1 cut(s) 1235
BstSCI CCNGG 2 cut(s) 1425, 1455
BstSFI CTRYAG 3 cut(s) 755, 1170, 1444
BstSNI TACGTA 1 cut(s) 890
BstV1I GCAGC 2 cut(s) 761, 1168
BstV2I GAAGAC 1 cut(s) 1302
BstX2I RGATCY 2 cut(s) 560, 1084
BstYI RGATCY 2 cut(s) 560, 1084
BsuI GTATCC 1 cut(s) 997
BsuRI GGCC 2 cut(s) 70, 1425
BtsCI GGATG 2 cut(s) 685, 1249
BtsI GCAGTG 2 cut(s) 699, 1453
BtsIMutI CAGTG 4 cut(s) 699, 1179, 1253, 1453
Cac8I GCNNGC 4 cut(s) 166, 483, 732, 1056
CaiI CAGNNNCTG 1 cut(s) 1142
CciI TCATGA 1 cut(s) 1649
CfoI GCGC 1 cut(s) 8
Cfr10I RCCGGY 1 cut(s) 211
CseI GACGC 3 cut(s) 160, 293, 812
CviAII CATG 6 cut(s) 66, 131, 596, 743, 1232, 1650
DdeI CTNAG 6 cut(s) 252, 368, 637, 1260, 1418, 1523
DpnI GATC 6 cut(s) 441, 562, 1065, 1086, 1454, 1499
DpnII GATC 6 cut(s) 439, 560, 1063, 1084, 1452, 1497
DriI GACNNNNNGTC 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 1423
Eam1105I GACNNNNNGTC 1 cut(s) 196
EciI GGCGGA 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 634
Eco105I TACGTA 1 cut(s) 890
Eco130I CCWWGG 2 cut(s) 990, 1319
Eco24I GRGCYC 1 cut(s) 636
Eco31I GGTCTC 2 cut(s) 218, 605
Eco32I GATATC 2 cut(s) 1209, 1243
Eco53kI GAGCTC 1 cut(s) 634
Eco57I CTGAAG 1 cut(s) 1171
EcoICRI GAGCTC 1 cut(s) 634
EcoRII CCWGG 2 cut(s) 1425, 1455
EcoRV GATATC 2 cut(s) 1209, 1243
EcoT14I CCWWGG 2 cut(s) 990, 1319
EcoT22I ATGCAT 2 cut(s) 601, 1646
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 2 cut(s) 990, 1319
FaeI CATG 6 cut(s) 69, 134, 599, 746, 1235, 1653
FalI AAGNNNNNCTT 6 cut(s) 869, 901, 1113, 1145, 1503, 1535
FaqI GGGAC 1 cut(s) 962
FatI CATG 6 cut(s) 65, 130, 595, 742, 1231, 1649
FauI CCCGC 1 cut(s) 723
Fnu4HI GCNGC 6 cut(s) 78, 169, 735, 750, 962, 1182
FokI GGATG 2 cut(s) 692, 1236
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 6 cut(s) 78, 169, 735, 750, 962, 1182
FspBI CTAG 5 cut(s) 482, 530, 920, 1082, 1301
GlaI GCGC 1 cut(s) 7
GluI GCNGC 6 cut(s) 78, 169, 735, 750, 962, 1182
GsaI CCCAGC 1 cut(s) 481
GsuI CTGGAG 1 cut(s) 1062
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 2 cut(s) 70, 1425
HapII CCGG 4 cut(s) 188, 212, 308, 952
HgaI GACGC 3 cut(s) 160, 293, 812
HhaI GCGC 1 cut(s) 8
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 6 cut(s) 69, 134, 599, 746, 1235, 1653
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HpaII CCGG 4 cut(s) 188, 212, 308, 952
HphI GGTGA 4 cut(s) 30, 229, 866, 1363
Hpy166II GTNNAC 4 cut(s) 810, 1002, 1282, 1580
Hpy8I GTNNAC 4 cut(s) 810, 1002, 1282, 1580
Hpy99I CGWCG 4 cut(s) 158, 176, 266, 316
HpyAV CCTTC 7 cut(s) 230, 325, 838, 907, 1092, 1195, 1462
HpyCH4III ACNGT 5 cut(s) 616, 1144, 1174, 1577, 1608
HpyCH4IV ACGT 3 cut(s) 427, 889, 1596
HpyCH4V TGCA 9 cut(s) 575, 590, 599, 1076, 1231, 1407, 1446, 1613, 1644
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 491, 596, 943, 1190
HpyF3I CTNAG 6 cut(s) 252, 368, 637, 1260, 1418, 1523
HpySE526I ACGT 3 cut(s) 427, 889, 1596
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 6 cut(s) 69, 134, 599, 746, 1235, 1653
HspAI GCGC 1 cut(s) 6
Kpn2I TCCGGA 1 cut(s) 187
Kzo9I GATC 6 cut(s) 439, 560, 1063, 1084, 1452, 1497
LmnI GCTCC 3 cut(s) 916, 1043, 1516
Lsp1109I GCAGC 2 cut(s) 761, 1168
LweI GCATC 1 cut(s) 1377
MaeI CTAG 5 cut(s) 482, 530, 920, 1082, 1301
MaeII ACGT 3 cut(s) 427, 889, 1596
MaeIII GTNAC 4 cut(s) 459, 692, 760, 1161
MalI GATC 6 cut(s) 441, 562, 1065, 1086, 1454, 1499
MboI GATC 6 cut(s) 439, 560, 1063, 1084, 1452, 1497
MboII GAAGA 7 cut(s) 245, 337, 340, 404, 1121, 1123, 1302
MfeI CAATTG 1 cut(s) 1034
MflI RGATCY 2 cut(s) 560, 1084
MhlI GDGCHC 2 cut(s) 423, 636
MlsI TGGCCA 1 cut(s) 1425
MluNI TGGCCA 1 cut(s) 1425
MlyI GAGTC 6 cut(s) 159, 267, 467, 805, 1223, 1466
MmeI TCCRAC 2 cut(s) 1112, 1522
Mox20I TGGCCA 1 cut(s) 1425
Mph1103I ATGCAT 2 cut(s) 601, 1646
MroI TCCGGA 1 cut(s) 187
MroXI GAANNNNTTC 2 cut(s) 359, 1128
MscI TGGCCA 1 cut(s) 1425
MseI TTAA 3 cut(s) 723, 1028, 1433
Msp20I TGGCCA 1 cut(s) 1425
MspA1I CMGCKG 2 cut(s) 730, 961
MspI CCGG 4 cut(s) 188, 212, 308, 952
MspR9I CCNGG 2 cut(s) 1427, 1457
MunI CAATTG 1 cut(s) 1034
MvaI CCWGG 2 cut(s) 1427, 1457
MwoI GCNNNNNNNGC 5 cut(s) 174, 491, 596, 943, 1190
NdeII GATC 6 cut(s) 439, 560, 1063, 1084, 1452, 1497
NheI GCTAGC 1 cut(s) 481
NlaIII CATG 6 cut(s) 69, 134, 599, 746, 1235, 1653
NlaIV GGNNCC 3 cut(s) 210, 514, 1045
NmeAIII GCCGAG 1 cut(s) 1084
NmuCI GTSAC 2 cut(s) 459, 692
NsiI ATGCAT 2 cut(s) 601, 1646
NspI RCATGY 1 cut(s) 1235
PagI TCATGA 1 cut(s) 1649
PdmI GAANNNNTTC 2 cut(s) 359, 1128
PfeI GAWTC 5 cut(s) 28, 103, 284, 923, 955
PfoI TCCNGGA 1 cut(s) 1455
PkrI GCNGC 6 cut(s) 79, 170, 736, 751, 963, 1183
PleI GAGTC 6 cut(s) 158, 266, 466, 805, 1222, 1466
PpsI GAGTC 6 cut(s) 158, 266, 466, 805, 1222, 1466
Ppu21I YACGTR 2 cut(s) 890, 1597
Psp124BI GAGCTC 1 cut(s) 636
Psp6I CCWGG 2 cut(s) 1425, 1455
PspFI CCCAGC 1 cut(s) 477
PspGI CCWGG 2 cut(s) 1425, 1455
PspN4I GGNNCC 3 cut(s) 210, 514, 1045
PsrI GAACNNNNNNTAC 2 cut(s) 1549, 1581
PstI CTGCAG 1 cut(s) 1448
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 2 cut(s) 560, 1084
SacI GAGCTC 1 cut(s) 636
SaqAI TTAA 3 cut(s) 723, 1028, 1433
SatI GCNGC 6 cut(s) 78, 169, 735, 750, 962, 1182
Sau3AI GATC 6 cut(s) 439, 560, 1063, 1084, 1452, 1497
ScaI AGTACT 1 cut(s) 1021
SchI GAGTC 6 cut(s) 159, 267, 467, 805, 1223, 1466
ScrFI CCNGG 2 cut(s) 1427, 1457
SduI GDGCHC 2 cut(s) 423, 636
SfaNI GCATC 1 cut(s) 1377
SfcI CTRYAG 3 cut(s) 755, 1170, 1444
SmlI CTYRAG 4 cut(s) 464, 818, 908, 1199
SmoI CTYRAG 4 cut(s) 464, 818, 908, 1199
SnaBI TACGTA 1 cut(s) 890
SpeI ACTAGT 1 cut(s) 529
SsiI CCGC 9 cut(s) 78, 110, 168, 215, 275, 697, 730, 734, 961
SspMI CTAG 5 cut(s) 482, 530, 920, 1082, 1301
SstI GAGCTC 1 cut(s) 636
StyD4I CCNGG 2 cut(s) 1425, 1455
StyI CCWWGG 2 cut(s) 990, 1319
TaaI ACNGT 5 cut(s) 616, 1144, 1174, 1577, 1608
TaiI ACGT 3 cut(s) 430, 892, 1599
TaqI TCGA 3 cut(s) 264, 793, 1313
TaqII GACCGA 1 cut(s) 242
TatI WGTACW 1 cut(s) 1019
TauI GCSGC 4 cut(s) 80, 171, 737, 964
TfiI GAWTC 5 cut(s) 28, 103, 284, 923, 955
Tru1I TTAA 3 cut(s) 723, 1028, 1433
Tru9I TTAA 3 cut(s) 723, 1028, 1433
TscAI CASTG 4 cut(s) 706, 1179, 1260, 1453
TseFI GTSAC 2 cut(s) 459, 692
TseI GCWGC 2 cut(s) 749, 1181
Tsp45I GTSAC 2 cut(s) 459, 692
TspDTI ATGAA 6 cut(s) 20, 54, 584, 759, 1124, 1141
TspGWI ACGGA 2 cut(s) 390, 562
TspRI CASTG 4 cut(s) 706, 1179, 1260, 1453
XapI RAATTY 2 cut(s) 541, 1124
XceI RCATGY 1 cut(s) 1235
XmnI GAANNNNTTC 2 cut(s) 359, 1128
XspI CTAG 5 cut(s) 482, 530, 920, 1082, 1301
ZrmI AGTACT 1 cut(s) 1021
Zsp2I ATGCAT 2 cut(s) 601, 1646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.