FvH4_2g39280

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003824
Physical Location & Seq
Forward (+)
28255098 .. 28259560
4463 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39280.t1

Sequence Viewer

Length: 1656 bp
ATGAGCGCGAGTCTGTGTTGCTCTCTGATTCGTTCCTCACCTCTACTTCACCACGAACCCTATCTCTCATGGCCTCTCGCCAAACTCAACCACCGCCAGATTTGGTCTCTCACGCTCCGAAACCATCCCCCTGCTTCTGCCAGAGTCGTCGCTAGAGCCGTCGCCGACGGCGGCGTCGGCGTTGTGTCGCCGGACGACTTGTCTTTTGAGGACGTGGCGGCGATTGAGACGGATGAGGTAGGAAATGGAGAGGTTGAGGGAGTCGTGGCGGAGAGTAAATCGGAGGATAAGGGTCTGCGTCCGACGACGAAATACAAGAAGAAGAAGAAGGAGGAGGAGGACGGGTTAGAGAACAGGTTCAAGCTGAGGAACGGAAAGGAGATTTTTGAAGAAAAAGCCTACATTGTTGGTGTTGAGCACAAACGTAATTCGACGGATTTATTTGGTGTGGAGGAGTCACTCAAGGAACTGACTCAGCTTGCTGATACTGCTGGACTAATGGTTGTTGGTTCCACATATCAGAAGCTAGCGTCTCCAAATTCAAGGACATACATTGGATCTGGTAAGGTTGCAGAAATCAAGAGTGCTATTCATGCATTGGGTGTCGAGACTGTGATATTTGATGATGAGCTCTCAGCTGGGCAACTACGCAATTTGGAAAAGGCTTTTGGTGGGGATGTTAGAGTTTGTGACCGCACTGCCCTTATTCTTGATATTTTCAACCAGCGGGCAGCAACTCATGAAGCAGCTCTACAGGTTGCATTGGCACAGATGGAATACCAGCTACCTCGACTAACAAAAATGTGGACTCATCTTGAGCGTCAATCAGGAGGTCAAGTGAAGGGTATGGGTGAGAAACAAATTGAAGTGGATAAGCGTATCTTACGTACTCAAATTGGTGTTCTCAAGAAGGAGCTAGAATCTGTTAGAAAGCATAGAAAGCAGTACCGGAATCAACGGCTTTCAGTACCAGTCCCAGTAGTATCCTTGGTTGGGTACACCAATGCTGGAAAGAGTACTCTTTTAAATCAATTGACTGGAGCCAATGTGCTTGCCGAGGATCAATTATTTGCAACACTAGATCCAACAACAAGAAGGGTTCAGATGAAGAATGGGATGGAATTTCTCCTCACAGATACTGTTGGTTTCATTCAGAAGTTACCAACCACAGTGGTTGCTGCCTTCAGAGCAACTCTTGAGGAGATATCGGAGTCGGCATTACTGGTGCATGTGGTTGATATCAGTCATCCACTGGCTGAGCAACAGATAGAAGCTGTGGACAGAGTGTTGTCAGAACTAGATGTCTCTTCTATACCAAGGTTGATGGTTTGGAACAAGGTTGATAAGGTGAGCAATCCTGACAGCATCAGAATAGAAGCAGAAAAGAGAGATGATGTTGTTTGCATATCTGCTCTGAGTGGTGAGGGCTTAAATGAATTCTGCAGTGCAATCCAGGAAAAATTGAAGGACTCTATGGTATGGGTGGAAGCCTTGATCCCTTTTGAAAAAGGAGAGCTTCTTAGTGCTATACATAAGGTTGGAATGGTGAAGAAAACAGAATATACCGAGCACGGGACACTGATCAAGGCACATGTACCTCTACGGTTTGCAAGGCTACTAACACCATTGAGGCAAATGTGCATATCAAGATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

552

Amino Acids

61.14

Weight (kDa)

6.73

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-bdg_N PF13167 151 - 240 1.7e-30 GTP-binding GTPase N-terminal
GTP-bdg_M PF16360 243 - 320 4.3e-27 GTP-binding GTPase Middle Region
MMR_HSR1 PF01926 328 - 446 1.3e-21 50S ribosome-binding GTPase
HflX_C PF19275 458 - 527 1.9e-08 HflX C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AccII CGCG 1 cut(s) 8
AciI CCGC 6 cut(s) 94, 171, 218, 269, 694, 727
AclWI GGATC 4 cut(s) 565, 1068, 1076, 1489
AcsI RAATTY 3 cut(s) 538, 1121, 1436
AcuI CTGAAG 1 cut(s) 1168
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 6 cut(s) 889, 947, 969, 998, 1018, 1596
AfiI CCNNNNNNNGG 2 cut(s) 993, 1572
AflIII ACRYGT 1 cut(s) 1591
AgsI TTSAA 7 cut(s) 361, 389, 543, 721, 866, 1465, 1505
AhdI GACNNNNNGTC 1 cut(s) 199
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 1 cut(s) 1452
AjuI GAANNNNNNNTTGG 2 cut(s) 651, 683
Alw21I GWGCWC 3 cut(s) 420, 633, 1572
Alw26I GTCTC 5 cut(s) 111, 221, 537, 602, 1309
AlwI GGATC 4 cut(s) 565, 1068, 1076, 1489
AlwNI CAGNNNCTG 1 cut(s) 1139
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 3 cut(s) 731, 746, 1178
ApoI RAATTY 3 cut(s) 538, 1121, 1436
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp700I GAANNNNTTC 1 cut(s) 356
AspLEI GCGC 1 cut(s) 8
AsuHPI GGTGA 6 cut(s) 30, 41, 863, 1360, 1433, 1558
AsuNHI GCTAGC 1 cut(s) 526
BanII GRGCYC 1 cut(s) 633
BarI GAAGNNNNNNTAC 2 cut(s) 735, 767
Bbv12I GWGCWC 3 cut(s) 420, 633, 1572
BbvCI CCTCAGC 1 cut(s) 365
BbvI GCAGC 3 cut(s) 743, 758, 1165
BccI CCATC 4 cut(s) 132, 766, 1111, 1318
BceAI ACGGC 3 cut(s) 143, 184, 974
BciT130I CCWGG 1 cut(s) 1454
BciVI GTATCC 1 cut(s) 994
BclI TGATCA 1 cut(s) 1581
BcoDI GTCTC 5 cut(s) 111, 221, 537, 602, 1309
BfaI CTAG 5 cut(s) 153, 527, 917, 1079, 1298
BfmI CTRYAG 2 cut(s) 752, 1441
BfuI GTATCC 1 cut(s) 994
BglII AGATCT 1 cut(s) 1649
BisI GCNGC 5 cut(s) 172, 219, 732, 747, 1179
BlpI GCTNAGC 1 cut(s) 1257
BlsI GCNGC 5 cut(s) 173, 220, 733, 748, 1180
BmcAI AGTACT 1 cut(s) 1018
Bme1390I CCNGG 1 cut(s) 1454
BmeRI GACNNNNNGTC 1 cut(s) 199
BmgBI CACGTC 1 cut(s) 214
BmiI GGNNCC 2 cut(s) 511, 1042
BmrFI CCNGG 1 cut(s) 1454
BmrI ACTGGG 1 cut(s) 971
BmsI GCATC 1 cut(s) 1374
BmtI GCTAGC 1 cut(s) 530
BmuI ACTGGG 1 cut(s) 971
BpmI CTGGAG 1 cut(s) 1059
Bpu10I CCTNAGC 1 cut(s) 365
Bpu1102I GCTNAGC 1 cut(s) 1257
BpuEI CTTGAG 4 cut(s) 446, 836, 890, 1217
BsaAI YACGTR 1 cut(s) 887
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 987, 1056, 1316
BsaWI WCCGGW 1 cut(s) 948
BsaXI ACNNNNNCTCC 6 cut(s) 329, 359, 822, 852, 1032, 1062
Bsc4I CCNNNNNNNGG 2 cut(s) 993, 1572
Bse1I ACTGG 5 cut(s) 971, 977, 1042, 1227, 1257
BseBI CCWGG 1 cut(s) 1454
BseDI CCNNGG 3 cut(s) 987, 1056, 1316
BseGI GGATG 5 cut(s) 124, 238, 682, 1122, 1246
BseLI CCNNNNNNNGG 2 cut(s) 993, 1572
BseMII CTCAG 5 cut(s) 356, 488, 648, 1248, 1406
BseNI ACTGG 5 cut(s) 971, 977, 1042, 1227, 1257
BseRI GAGGAG 5 cut(s) 347, 350, 467, 1118, 1214
BseXI GCAGC 3 cut(s) 743, 758, 1165
BseYI CCCAGC 1 cut(s) 638
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 1 cut(s) 73
BsiHKAI GWGCWC 3 cut(s) 420, 633, 1572
BsiSI CCGG 2 cut(s) 191, 949
BslFI GGGAC 2 cut(s) 959, 1588
BslI CCNNNNNNNGG 2 cut(s) 993, 1572
BsmAI GTCTC 5 cut(s) 111, 221, 537, 602, 1309
BsmBI CGTCTC 2 cut(s) 221, 537
BsmFI GGGAC 2 cut(s) 959, 1588
BsnI GGCC 1 cut(s) 73
Bso31I GGTCTC 1 cut(s) 111
Bsp1286I GDGCHC 3 cut(s) 420, 633, 1572
Bsp143I GATC 6 cut(s) 557, 1060, 1081, 1494, 1581, 1649
Bsp1720I GCTNAGC 1 cut(s) 1257
BspACI CCGC 6 cut(s) 94, 171, 218, 269, 694, 727
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 5 cut(s) 357, 487, 647, 1249, 1407
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 2 cut(s) 511, 1042
BspMAI CTGCAG 1 cut(s) 1445
BspOI GCTAGC 1 cut(s) 530
BspPI GGATC 4 cut(s) 565, 1068, 1076, 1489
BspTNI GGTCTC 1 cut(s) 111
BsrI ACTGG 5 cut(s) 971, 977, 1042, 1227, 1257
BssECI CCNNGG 3 cut(s) 987, 1056, 1316
BssMI GATC 6 cut(s) 557, 1060, 1081, 1494, 1581, 1649
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 2 cut(s) 987, 1316
Bst2UI CCWGG 1 cut(s) 1454
Bst4CI ACNGT 4 cut(s) 613, 1141, 1171, 1605
Bst6I CTCTTC 1 cut(s) 1312
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 4 cut(s) 480, 528, 729, 1053
BstDEI CTNAG 6 cut(s) 365, 474, 634, 1257, 1415, 1520
BstF5I GGATG 5 cut(s) 124, 238, 682, 1122, 1246
BstFNI CGCG 1 cut(s) 8
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 6 cut(s) 560, 1063, 1084, 1497, 1584, 1652
BstMAI GTCTC 5 cut(s) 111, 221, 537, 602, 1309
BstMBI GATC 6 cut(s) 557, 1060, 1081, 1494, 1581, 1649
BstMWI GCNNNNNNNGC 5 cut(s) 177, 488, 593, 940, 1187
BstNI CCWGG 1 cut(s) 1454
BstNSI RCATGY 2 cut(s) 1232, 1595
BstSCI CCNGG 1 cut(s) 1452
BstSFI CTRYAG 2 cut(s) 752, 1441
BstSNI TACGTA 1 cut(s) 887
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 3 cut(s) 743, 758, 1165
BstX2I RGATCY 3 cut(s) 557, 1081, 1649
BstYI RGATCY 3 cut(s) 557, 1081, 1649
BsuI GTATCC 1 cut(s) 994
BsuRI GGCC 1 cut(s) 73
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 5 cut(s) 124, 238, 682, 1122, 1246
BtsI GCAGTG 2 cut(s) 696, 1450
BtsIMutI CAGTG 5 cut(s) 696, 1176, 1250, 1450, 1577
Cac8I GCNNGC 4 cut(s) 480, 528, 729, 1053
CaiI CAGNNNCTG 1 cut(s) 1139
CciI TCATGA 1 cut(s) 739
CfoI GCGC 1 cut(s) 8
CseI GACGC 4 cut(s) 163, 287, 519, 809
Csp6I GTAC 6 cut(s) 888, 946, 968, 997, 1017, 1595
CspCI CAANNNNNGTGG 4 cut(s) 1152, 1156, 1187, 1191
CviAII CATG 5 cut(s) 69, 593, 740, 1229, 1592
CviQI GTAC 6 cut(s) 888, 946, 968, 997, 1017, 1595
DdeI CTNAG 6 cut(s) 365, 474, 634, 1257, 1415, 1520
DpnI GATC 6 cut(s) 559, 1062, 1083, 1496, 1583, 1651
DpnII GATC 6 cut(s) 557, 1060, 1081, 1494, 1581, 1649
DraI TTTAAA 1 cut(s) 1026
DrdI GACNNNNNNGTC 1 cut(s) 173
DriI GACNNNNNGTC 1 cut(s) 199
DseDI GACNNNNNNGTC 1 cut(s) 173
Eam1104I CTCTTC 1 cut(s) 1312
Eam1105I GACNNNNNGTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 1312
EciI GGCGGA 1 cut(s) 284
Ecl136II GAGCTC 1 cut(s) 631
Eco105I TACGTA 1 cut(s) 887
Eco130I CCWWGG 2 cut(s) 987, 1316
Eco24I GRGCYC 1 cut(s) 633
Eco31I GGTCTC 1 cut(s) 111
Eco32I GATATC 2 cut(s) 1206, 1240
Eco53kI GAGCTC 1 cut(s) 631
Eco57I CTGAAG 1 cut(s) 1168
EcoICRI GAGCTC 1 cut(s) 631
EcoRI GAATTC 1 cut(s) 1436
EcoRII CCWGG 1 cut(s) 1452
EcoRV GATATC 2 cut(s) 1206, 1240
EcoT14I CCWWGG 2 cut(s) 987, 1316
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 633
ErhI CCWWGG 2 cut(s) 987, 1316
Esp3I CGTCTC 2 cut(s) 221, 537
FaeI CATG 5 cut(s) 72, 596, 743, 1232, 1595
FalI AAGNNNNNCTT 4 cut(s) 866, 898, 1500, 1532
FaqI GGGAC 2 cut(s) 959, 1588
FatI CATG 5 cut(s) 68, 592, 739, 1228, 1591
FauI CCCGC 1 cut(s) 720
FbaI TGATCA 1 cut(s) 1581
Fnu4HI GCNGC 5 cut(s) 172, 219, 732, 747, 1179
FokI GGATG 5 cut(s) 111, 245, 689, 1129, 1233
FriOI GRGCYC 1 cut(s) 633
Fsp4HI GCNGC 5 cut(s) 172, 219, 732, 747, 1179
FspBI CTAG 5 cut(s) 153, 527, 917, 1079, 1298
GlaI GCGC 1 cut(s) 7
GluI GCNGC 5 cut(s) 172, 219, 732, 747, 1179
GsaI CCCAGC 1 cut(s) 642
GsuI CTGGAG 1 cut(s) 1059
HaeIII GGCC 1 cut(s) 73
HapII CCGG 2 cut(s) 191, 949
HgaI GACGC 4 cut(s) 163, 287, 519, 809
HhaI GCGC 1 cut(s) 8
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 5 cut(s) 72, 596, 743, 1232, 1595
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HpaII CCGG 2 cut(s) 191, 949
HphI GGTGA 6 cut(s) 30, 41, 863, 1360, 1433, 1558
Hpy166II GTNNAC 3 cut(s) 807, 999, 1279
Hpy8I GTNNAC 3 cut(s) 807, 999, 1279
Hpy99I CGWCG 7 cut(s) 152, 164, 170, 179, 307, 310, 436
HpyAV CCTTC 6 cut(s) 322, 835, 904, 1089, 1192, 1459
HpyCH4III ACNGT 4 cut(s) 613, 1141, 1171, 1605
HpyCH4IV ACGT 3 cut(s) 213, 424, 886
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 488, 593, 940, 1187
HpyF3I CTNAG 6 cut(s) 365, 474, 634, 1257, 1415, 1520
HpySE526I ACGT 3 cut(s) 213, 424, 886
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 5 cut(s) 72, 596, 743, 1232, 1595
HspAI GCGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 1581
Kzo9I GATC 6 cut(s) 557, 1060, 1081, 1494, 1581, 1649
LmnI GCTCC 3 cut(s) 120, 913, 1040
Lsp1109I GCAGC 3 cut(s) 743, 758, 1165
LweI GCATC 1 cut(s) 1374
MaeI CTAG 5 cut(s) 153, 527, 917, 1079, 1298
MaeII ACGT 3 cut(s) 213, 424, 886
MaeIII GTNAC 3 cut(s) 456, 689, 1158
MalI GATC 6 cut(s) 559, 1062, 1083, 1496, 1583, 1651
MboI GATC 6 cut(s) 557, 1060, 1081, 1494, 1581, 1649
MboII GAAGA 7 cut(s) 331, 334, 337, 401, 1120, 1299, 1561
MfeI CAATTG 1 cut(s) 1031
MflI RGATCY 3 cut(s) 557, 1081, 1649
MhlI GDGCHC 3 cut(s) 420, 633, 1572
MlyI GAGTC 8 cut(s) 19, 153, 270, 464, 466, 802, 1220, 1463
MmeI TCCRAC 3 cut(s) 326, 1109, 1519
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 3 cut(s) 1025, 1430, 1654
MspA1I CMGCKG 2 cut(s) 638, 727
MspI CCGG 2 cut(s) 191, 949
MspR9I CCNGG 1 cut(s) 1454
MunI CAATTG 1 cut(s) 1031
MvaI CCWGG 1 cut(s) 1454
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 5 cut(s) 177, 488, 593, 940, 1187
NdeII GATC 6 cut(s) 557, 1060, 1081, 1494, 1581, 1649
NheI GCTAGC 1 cut(s) 526
NlaIII CATG 5 cut(s) 72, 596, 743, 1232, 1595
NlaIV GGNNCC 2 cut(s) 511, 1042
NmeAIII GCCGAG 1 cut(s) 1081
NmuCI GTSAC 2 cut(s) 456, 689
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 2 cut(s) 1232, 1595
PagI TCATGA 1 cut(s) 739
PciI ACATGT 1 cut(s) 1591
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 3 cut(s) 28, 920, 952
PfoI TCCNGGA 1 cut(s) 1452
PkrI GCNGC 5 cut(s) 173, 220, 733, 748, 1180
PleI GAGTC 8 cut(s) 18, 152, 269, 463, 466, 802, 1219, 1463
PpsI GAGTC 8 cut(s) 18, 152, 269, 463, 466, 802, 1219, 1463
Ppu21I YACGTR 1 cut(s) 887
PscI ACATGT 1 cut(s) 1591
Psp124BI GAGCTC 1 cut(s) 633
Psp6I CCWGG 1 cut(s) 1452
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 1 cut(s) 1452
PspN4I GGNNCC 2 cut(s) 511, 1042
PstI CTGCAG 1 cut(s) 1445
PstNI CAGNNNCTG 1 cut(s) 1139
PsuI RGATCY 3 cut(s) 557, 1081, 1649
PvuII CAGCTG 1 cut(s) 638
RsaI GTAC 6 cut(s) 889, 947, 969, 998, 1018, 1596
RsaNI GTAC 6 cut(s) 888, 946, 968, 997, 1017, 1595
SacI GAGCTC 1 cut(s) 633
SaqAI TTAA 3 cut(s) 1025, 1430, 1654
SatI GCNGC 5 cut(s) 172, 219, 732, 747, 1179
Sau3AI GATC 6 cut(s) 557, 1060, 1081, 1494, 1581, 1649
ScaI AGTACT 1 cut(s) 1018
SchI GAGTC 8 cut(s) 19, 153, 270, 464, 466, 802, 1220, 1463
ScrFI CCNGG 1 cut(s) 1454
SduI GDGCHC 3 cut(s) 420, 633, 1572
SfaNI GCATC 1 cut(s) 1374
SfcI CTRYAG 2 cut(s) 752, 1441
SmlI CTYRAG 4 cut(s) 461, 815, 905, 1196
SmoI CTYRAG 4 cut(s) 461, 815, 905, 1196
SnaBI TACGTA 1 cut(s) 887
SsiI CCGC 6 cut(s) 94, 171, 218, 269, 694, 727
SspMI CTAG 5 cut(s) 153, 527, 917, 1079, 1298
SstI GAGCTC 1 cut(s) 633
StyD4I CCNGG 1 cut(s) 1452
StyI CCWWGG 2 cut(s) 987, 1316
TaaI ACNGT 4 cut(s) 613, 1141, 1171, 1605
TaiI ACGT 3 cut(s) 216, 427, 889
TaqI TCGA 3 cut(s) 431, 606, 790
TatI WGTACW 1 cut(s) 1016
TauI GCSGC 2 cut(s) 174, 221
TfiI GAWTC 3 cut(s) 28, 920, 952
Tru1I TTAA 3 cut(s) 1025, 1430, 1654
Tru9I TTAA 3 cut(s) 1025, 1430, 1654
TscAI CASTG 5 cut(s) 703, 1176, 1257, 1450, 1584
TseFI GTSAC 2 cut(s) 456, 689
TseI GCWGC 3 cut(s) 731, 746, 1178
Tsp45I GTSAC 2 cut(s) 456, 689
TspDTI ATGAA 5 cut(s) 581, 756, 1121, 1138, 1449
TspGWI ACGGA 3 cut(s) 245, 387, 449
TspRI CASTG 5 cut(s) 703, 1176, 1257, 1450, 1584
XapI RAATTY 3 cut(s) 538, 1121, 1436
XceI RCATGY 2 cut(s) 1232, 1595
XmnI GAANNNNTTC 1 cut(s) 356
XspI CTAG 5 cut(s) 153, 527, 917, 1079, 1298
ZrmI AGTACT 1 cut(s) 1018
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.