Rh6DG421100

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003824
Physical Location & Seq
Reverse (-)
60293682 .. 60298730
5049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG421100.1

Sequence Viewer

Length: 1269 bp
ATGAGCACCAGTTTGTGTTGCTCTCTGATTCGTTCCTCACCTCTGCTTCCCGAACCCTATCTCTCATGGCCTCTCGCCAAACCTAACCACCACCGCCAGATTCAACCGCCTCTCACGCTCAGAAACCATTCCCGTGCTTCTGCGAGAGTCGTCGCAAGAGCTGTCGCCGACGGCGTTGTGTCTCCGGACGACTTGTCGTTTCAGGAACCGGCGGTGATTGAGACCGAGGAAGAAGGAAATGGAGAGGCTCAGGGAGTCGTCGATGGAGTGGCGGAGAGTGAATCAGAGGATAATAAGGGTGTGCGTCCGGCGACGAGGTACAAGAAGAAGAAGGAGGATGAGGACGGGTTAGAGAATAGGTTCAAGCTGAGGAACGGAAAGGAGATTTTTGAAGAAAAAGCCTACATTGTGGGTGTTGAGCACAAACGTAATTCGGCTGATCTATTTGGTGTAGAGGAGTCACTCAAGGAATTGACCCAGCTAGCTGATACTGCTGGACTAATGGTTGTTGGTTCCACATATCAGAAACTAGTGTCTCCAAATTCACGGACATACATTGGATCTGGCAAGGTTGCAGAAATCAAGAGTGCAATTCATGCATTGGGTGTTGAGACTGTGATATTTGATGATGAGCTCTCAGCAGGGCAATTACGCAATTTGGAAAAGGCTTTTGGTGGGGATGTTAGAGTCTGTGACCGCACTGCTCTCATTCTTGATATTTTTAACCAGCGGGCGGCAACACATGAAGCAGCTCTACAGGTTACATTGGCACAGATGGAATACCAGCTACCTCGACTAACAAAAATGTGGACTCATCTTGAGCGTCAATCAGGAGGTCAAGTGAAGGGTATGGGTGAGAAACAAATTGAAGTGGATAAGCGTATCTTACGTACTCAAATTGGTGTTCTCAAGAAGGAGCTAGAATCTGTTAGAAAGCATAGAAAGCAGTACCGGAATCAGCGGCTTTCAGTACCAGTACCAGTAGTATCCTTGGTTGGGTACACCAATGCTGGAAAGAGTACTCTCTTAAATCAATTGACTGGAGCCAATGTGCTTGCCGAGGATCAATTATTTGCAACACTAGATCCAACCACAAGAAGGGTTCAGATGAAGAACGGGAAAGAATTTCTTCTCACAGATACTGTTGGTTTCATTCAGAAGTTACCGACTACAGTGGTTGCTGCCTTCAGAGCAACTCTTGAGGAGATATCAGAGTCAGCATTACTGGTGCATGTGGTTGATATCAGGTACAAAGTGGCATCCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

422

Amino Acids

46.79

Weight (kDa)

7.72

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-bdg_N PF13167 152 - 241 2.7e-30 GTP-binding GTPase N-terminal
GTP-bdg_M PF16360 244 - 321 3.9e-27 GTP-binding GTPase Middle Region
MMR_HSR1 PF01926 329 - 416 3.7e-19 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 8 cut(s) 94, 107, 212, 272, 697, 730, 734, 961
AclWI GGATC 3 cut(s) 568, 1071, 1079
AcsI RAATTY 2 cut(s) 541, 1124
AcuI CTGAAG 1 cut(s) 1171
AfaI GTAC 8 cut(s) 320, 892, 950, 972, 978, 1001, 1021, 1250
AfiI CCNNNNNNNGG 3 cut(s) 733, 996, 1098
AgsI TTSAA 4 cut(s) 104, 364, 392, 869
AhdI GACNNNNNGTC 1 cut(s) 193
AhlI ACTAGT 1 cut(s) 529
AjuI GAANNNNNNNTTGG 2 cut(s) 654, 686
AluBI AGCT 8 cut(s) 161, 367, 481, 485, 634, 752, 787, 919
AluI AGCT 8 cut(s) 161, 367, 481, 485, 634, 752, 787, 919
Alw21I GWGCWC 3 cut(s) 8, 423, 636
Alw26I GTCTC 4 cut(s) 186, 215, 540, 605
AlwI GGATC 3 cut(s) 568, 1071, 1079
AlwNI CAGNNNCTG 1 cut(s) 1142
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 2 cut(s) 749, 1181
ApoI RAATTY 2 cut(s) 541, 1124
Asp700I GAANNNNTTC 3 cut(s) 127, 359, 1128
AsuHPI GGTGA 3 cut(s) 30, 226, 866
AsuNHI GCTAGC 1 cut(s) 481
BanII GRGCYC 1 cut(s) 636
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
Bbv12I GWGCWC 3 cut(s) 8, 423, 636
BbvCI CCTCAGC 1 cut(s) 368
BbvI GCAGC 2 cut(s) 761, 1168
BccI CCATC 2 cut(s) 257, 769
BceAI ACGGC 1 cut(s) 187
BciVI GTATCC 1 cut(s) 997
BcoDI GTCTC 4 cut(s) 186, 215, 540, 605
BcuI ACTAGT 1 cut(s) 529
BfaI CTAG 4 cut(s) 482, 530, 920, 1082
BfmI CTRYAG 2 cut(s) 755, 1170
BfuI GTATCC 1 cut(s) 997
BisI GCNGC 4 cut(s) 735, 750, 962, 1182
BlsI GCNGC 4 cut(s) 736, 751, 963, 1183
BmcAI AGTACT 1 cut(s) 1021
BmeRI GACNNNNNGTC 1 cut(s) 193
BmiI GGNNCC 3 cut(s) 207, 514, 1045
BmtI GCTAGC 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 1062
Bpu10I CCTNAGC 2 cut(s) 249, 368
BpuEI CTTGAG 4 cut(s) 449, 839, 893, 1220
BsaAI YACGTR 1 cut(s) 890
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 3 cut(s) 225, 990, 1059
BsaWI WCCGGW 2 cut(s) 184, 951
BsaXI ACNNNNNCTCC 4 cut(s) 825, 855, 1035, 1065
Bsc4I CCNNNNNNNGG 3 cut(s) 733, 996, 1098
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 5 cut(s) 9, 974, 980, 1045, 1230
BseAI TCCGGA 1 cut(s) 184
BseDI CCNNGG 3 cut(s) 225, 990, 1059
BseGI GGATG 3 cut(s) 343, 685, 1259
BseLI CCNNNNNNNGG 3 cut(s) 733, 996, 1098
BseMII CTCAG 4 cut(s) 133, 263, 359, 651
BseNI ACTGG 5 cut(s) 9, 974, 980, 1045, 1230
BseRI GAGGAG 2 cut(s) 470, 1217
BseXI GCAGC 2 cut(s) 761, 1168
BseYI CCCAGC 1 cut(s) 477
BshFI GGCC 1 cut(s) 70
BsiHKAI GWGCWC 3 cut(s) 8, 423, 636
BsiSI CCGG 4 cut(s) 185, 209, 308, 952
BslI CCNNNNNNNGG 3 cut(s) 733, 996, 1098
BsmAI GTCTC 4 cut(s) 186, 215, 540, 605
BsnI GGCC 1 cut(s) 70
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 3 cut(s) 8, 423, 636
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 4 cut(s) 439, 560, 1063, 1084
BspACI CCGC 8 cut(s) 94, 107, 212, 272, 697, 730, 734, 961
BspANI GGCC 1 cut(s) 70
BspCNI CTCAG 4 cut(s) 132, 262, 360, 650
BspEI TCCGGA 1 cut(s) 184
BspLI GGNNCC 3 cut(s) 207, 514, 1045
BspOI GCTAGC 1 cut(s) 485
BspPI GGATC 3 cut(s) 568, 1071, 1079
BspTNI GGTCTC 1 cut(s) 215
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 5 cut(s) 9, 974, 980, 1045, 1230
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 3 cut(s) 225, 990, 1059
BssMI GATC 4 cut(s) 439, 560, 1063, 1084
BssT1I CCWWGG 1 cut(s) 990
Bst4CI ACNGT 3 cut(s) 616, 1144, 1174
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBAI YACGTR 1 cut(s) 890
BstC8I GCNNGC 3 cut(s) 483, 732, 1056
BstDEI CTNAG 4 cut(s) 119, 249, 368, 637
BstF5I GGATG 3 cut(s) 343, 685, 1259
BstKTI GATC 4 cut(s) 442, 563, 1066, 1087
BstMAI GTCTC 4 cut(s) 186, 215, 540, 605
BstMBI GATC 4 cut(s) 439, 560, 1063, 1084
BstMWI GCNNNNNNNGC 5 cut(s) 115, 491, 596, 943, 1190
BstNSI RCATGY 1 cut(s) 1235
BstSFI CTRYAG 2 cut(s) 755, 1170
BstSNI TACGTA 1 cut(s) 890
BstV1I GCAGC 2 cut(s) 761, 1168
BstX2I RGATCY 2 cut(s) 560, 1084
BstYI RGATCY 2 cut(s) 560, 1084
BsuI GTATCC 1 cut(s) 997
BsuRI GGCC 1 cut(s) 70
BtsCI GGATG 3 cut(s) 343, 685, 1259
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 2 cut(s) 699, 1179
Cac8I GCNNGC 3 cut(s) 483, 732, 1056
CaiI CAGNNNCTG 1 cut(s) 1142
Cfr10I RCCGGY 1 cut(s) 208
CseI GACGC 2 cut(s) 293, 812
Csp6I GTAC 8 cut(s) 319, 891, 949, 971, 977, 1000, 1020, 1249
CviAII CATG 4 cut(s) 66, 596, 743, 1232
CviQI GTAC 8 cut(s) 319, 891, 949, 971, 977, 1000, 1020, 1249
DdeI CTNAG 4 cut(s) 119, 249, 368, 637
DpnI GATC 4 cut(s) 441, 562, 1065, 1086
DpnII GATC 4 cut(s) 439, 560, 1063, 1084
DriI GACNNNNNGTC 1 cut(s) 193
Eam1105I GACNNNNNGTC 1 cut(s) 193
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 634
Eco105I TACGTA 1 cut(s) 890
Eco130I CCWWGG 1 cut(s) 990
Eco24I GRGCYC 1 cut(s) 636
Eco31I GGTCTC 1 cut(s) 215
Eco32I GATATC 2 cut(s) 1209, 1243
Eco53kI GAGCTC 1 cut(s) 634
Eco57I CTGAAG 1 cut(s) 1171
EcoICRI GAGCTC 1 cut(s) 634
EcoRV GATATC 2 cut(s) 1209, 1243
EcoT14I CCWWGG 1 cut(s) 990
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 990
FaeI CATG 4 cut(s) 69, 599, 746, 1235
FaiI YATR 8 cut(s) 67, 520, 553, 597, 744, 851, 939, 1233
FalI AAGNNNNNCTT 4 cut(s) 869, 901, 1113, 1145
FatI CATG 4 cut(s) 65, 595, 742, 1231
FauI CCCGC 1 cut(s) 723
Fnu4HI GCNGC 4 cut(s) 735, 750, 962, 1182
FokI GGATG 3 cut(s) 350, 692, 1246
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 4 cut(s) 735, 750, 962, 1182
FspBI CTAG 4 cut(s) 482, 530, 920, 1082
GluI GCNGC 4 cut(s) 735, 750, 962, 1182
GsaI CCCAGC 1 cut(s) 481
GsuI CTGGAG 1 cut(s) 1062
HaeIII GGCC 1 cut(s) 70
HapII CCGG 4 cut(s) 185, 209, 308, 952
HgaI GACGC 2 cut(s) 293, 812
Hin1II CATG 4 cut(s) 69, 599, 746, 1235
HpaII CCGG 4 cut(s) 185, 209, 308, 952
HphI GGTGA 3 cut(s) 30, 226, 866
Hpy166II GTNNAC 2 cut(s) 810, 1002
Hpy188I TCNGA 8 cut(s) 27, 122, 286, 525, 1107, 1158, 1190, 1213
Hpy188III TCNNGA 9 cut(s) 50, 185, 203, 583, 713, 818, 831, 910, 1199
Hpy8I GTNNAC 2 cut(s) 810, 1002
Hpy99I CGWCG 4 cut(s) 155, 173, 263, 316
HpyAV CCTTC 6 cut(s) 227, 325, 838, 907, 1092, 1195
HpyCH4III ACNGT 3 cut(s) 616, 1144, 1174
HpyCH4IV ACGT 2 cut(s) 427, 889
HpyCH4V TGCA 5 cut(s) 575, 590, 599, 1076, 1231
HpyF10VI GCNNNNNNNGC 5 cut(s) 115, 491, 596, 943, 1190
HpyF3I CTNAG 4 cut(s) 119, 249, 368, 637
HpySE526I ACGT 2 cut(s) 427, 889
Hsp92II CATG 4 cut(s) 69, 599, 746, 1235
Kpn2I TCCGGA 1 cut(s) 184
Kzo9I GATC 4 cut(s) 439, 560, 1063, 1084
LmnI GCTCC 2 cut(s) 916, 1043
Lsp1109I GCAGC 2 cut(s) 761, 1168
MaeI CTAG 4 cut(s) 482, 530, 920, 1082
MaeII ACGT 2 cut(s) 427, 889
MaeIII GTNAC 4 cut(s) 459, 692, 760, 1161
MalI GATC 4 cut(s) 441, 562, 1065, 1086
MboI GATC 4 cut(s) 439, 560, 1063, 1084
MboII GAAGA 6 cut(s) 242, 337, 340, 404, 1121, 1123
MfeI CAATTG 1 cut(s) 1034
MflI RGATCY 2 cut(s) 560, 1084
MhlI GDGCHC 3 cut(s) 8, 423, 636
MlyI GAGTC 6 cut(s) 156, 264, 467, 696, 805, 1223
MmeI TCCRAC 1 cut(s) 1112
Mph1103I ATGCAT 1 cut(s) 601
MroI TCCGGA 1 cut(s) 184
MroXI GAANNNNTTC 3 cut(s) 127, 359, 1128
MseI TTAA 3 cut(s) 723, 1028, 1267
MslI CAYNNNNRTG 1 cut(s) 132
MspA1I CMGCKG 2 cut(s) 730, 961
MspI CCGG 4 cut(s) 185, 209, 308, 952
MunI CAATTG 1 cut(s) 1034
MwoI GCNNNNNNNGC 5 cut(s) 115, 491, 596, 943, 1190
NdeII GATC 4 cut(s) 439, 560, 1063, 1084
NheI GCTAGC 1 cut(s) 481
NlaIII CATG 4 cut(s) 69, 599, 746, 1235
NlaIV GGNNCC 3 cut(s) 207, 514, 1045
NmeAIII GCCGAG 1 cut(s) 1084
NmuCI GTSAC 2 cut(s) 459, 692
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 1 cut(s) 1235
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PdmI GAANNNNTTC 3 cut(s) 127, 359, 1128
PfeI GAWTC 5 cut(s) 28, 100, 281, 923, 955
PkrI GCNGC 4 cut(s) 736, 751, 963, 1183
PleI GAGTC 6 cut(s) 155, 263, 466, 695, 805, 1222
PpsI GAGTC 6 cut(s) 155, 263, 466, 695, 805, 1222
Ppu21I YACGTR 1 cut(s) 890
Psp124BI GAGCTC 1 cut(s) 636
PspFI CCCAGC 1 cut(s) 477
PspN4I GGNNCC 3 cut(s) 207, 514, 1045
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 2 cut(s) 560, 1084
RsaI GTAC 8 cut(s) 320, 892, 950, 972, 978, 1001, 1021, 1250
RsaNI GTAC 8 cut(s) 319, 891, 949, 971, 977, 1000, 1020, 1249
RseI CAYNNNNRTG 1 cut(s) 132
SacI GAGCTC 1 cut(s) 636
SaqAI TTAA 3 cut(s) 723, 1028, 1267
SatI GCNGC 4 cut(s) 735, 750, 962, 1182
Sau3AI GATC 4 cut(s) 439, 560, 1063, 1084
ScaI AGTACT 1 cut(s) 1021
SchI GAGTC 6 cut(s) 156, 264, 467, 696, 805, 1223
SduI GDGCHC 3 cut(s) 8, 423, 636
SfcI CTRYAG 2 cut(s) 755, 1170
SmiMI CAYNNNNRTG 1 cut(s) 132
SmlI CTYRAG 4 cut(s) 464, 818, 908, 1199
SmoI CTYRAG 4 cut(s) 464, 818, 908, 1199
SnaBI TACGTA 1 cut(s) 890
SpeI ACTAGT 1 cut(s) 529
SsiI CCGC 8 cut(s) 94, 107, 212, 272, 697, 730, 734, 961
SspMI CTAG 4 cut(s) 482, 530, 920, 1082
SstI GAGCTC 1 cut(s) 636
StyI CCWWGG 1 cut(s) 990
TaaI ACNGT 3 cut(s) 616, 1144, 1174
TaiI ACGT 2 cut(s) 430, 892
TaqI TCGA 2 cut(s) 261, 793
TaqII GACCGA 1 cut(s) 239
TatI WGTACW 1 cut(s) 1019
TauI GCSGC 2 cut(s) 737, 964
TfiI GAWTC 5 cut(s) 28, 100, 281, 923, 955
Tru1I TTAA 3 cut(s) 723, 1028, 1267
Tru9I TTAA 3 cut(s) 723, 1028, 1267
TscAI CASTG 2 cut(s) 706, 1179
TseFI GTSAC 2 cut(s) 459, 692
TseI GCWGC 2 cut(s) 749, 1181
Tsp45I GTSAC 2 cut(s) 459, 692
TspDTI ATGAA 4 cut(s) 584, 759, 1124, 1141
TspGWI ACGGA 2 cut(s) 390, 562
TspRI CASTG 2 cut(s) 706, 1179
XapI RAATTY 2 cut(s) 541, 1124
XceI RCATGY 1 cut(s) 1235
XmnI GAANNNNTTC 3 cut(s) 127, 359, 1128
XspI CTAG 4 cut(s) 482, 530, 920, 1082
ZrmI AGTACT 1 cut(s) 1021
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.