Rorug05G0101700

GTP-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003824
Physical Location & Seq
Forward (+)
8965521 .. 8966687
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0101700.1

Sequence Viewer

Length: 534 bp
ATGGGGGGATTTGGAGATAATCTCAAATTATATGACTTGCATCAAGCTGATACTCCGATATGGCAAATTGCAAAACTTGATGCATGTGTTACATCTGTTGCATGGGTCGACAGCTATAGACTAATGGTTTCTTTTAGAGATAAAGTGGGTTTTAGAGTATGGGATATAAGAGATCCTTCTTGGATACAACAGATTCGGACTCATTCATCTGTGACAAGTATTGAACTGGACCAAAATAAGAATTACGTCACAACGATGAACGGAGACACTGCTACTATTTGGAACCTTACATTTAACCCTGTGGTAGAGCTATATATGCCACGCTTATCAGAATTAGCCTATTTCAGACCACAAAATGATATTTTGATTGTTGGAGGAGATGAAAGATTGGTTCATATACTGAACATCAATGATTGTACTGAGATTGATGCCGTAGGTGATGGGTTTCACATGGGTCCCATTCAGTGTGTTAGCTTCTCTCCAAATGGCAAGTCATATGCAGCAAGATCCCGAGACGGAAGAACGATAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP-bdg_M GTP-bdg_N MMR_HSR1

Protein Analysis

177

Amino Acids

20.07

Weight (kDa)

5.25

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 2 - 173 4.1e-12 Prp19 WD40 domain
EIF3I PF24805 14 - 173 2.8e-06 EIF3I
WD40_WDHD1_1st PF24817 23 - 176 3.3e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 79 - 174 9.9e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 108
AclWI GGATC 2 cut(s) 167, 501
AfaI GTAC 1 cut(s) 418
AgsI TTSAA 1 cut(s) 224
AluBI AGCT 4 cut(s) 47, 114, 310, 474
AluI AGCT 4 cut(s) 47, 114, 310, 474
Alw26I GTCTC 2 cut(s) 258, 507
AlwI GGATC 2 cut(s) 167, 501
Ama87I CYCGRG 1 cut(s) 510
ApeKI GCWGC 1 cut(s) 500
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
AspS9I GGNCC 2 cut(s) 229, 455
AsuHPI GGTGA 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 510
AvaII GGWCC 2 cut(s) 229, 455
BbvI GCAGC 1 cut(s) 512
BccI CCATC 1 cut(s) 434
BceAI ACGGC 1 cut(s) 416
BciVI GTATCC 1 cut(s) 177
BcoDI GTCTC 2 cut(s) 258, 507
BfmI CTRYAG 1 cut(s) 115
BfuI GTATCC 1 cut(s) 177
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
Bme18I GGWCC 2 cut(s) 229, 455
BmeT110I CYCGRG 1 cut(s) 510
BmgT120I GGNCC 2 cut(s) 229, 455
BmiI GGNNCC 3 cut(s) 284, 456, 457
BmsI GCATC 3 cut(s) 49, 70, 418
BplI GAGNNNNNCTC 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 231
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 1 cut(s) 390
BseXI GCAGC 1 cut(s) 512
BsiHKCI CYCGRG 1 cut(s) 510
BslFI GGGAC 1 cut(s) 441
BsmAI GTCTC 2 cut(s) 258, 507
BsmBI CGTCTC 1 cut(s) 507
BsmFI GGGAC 1 cut(s) 441
BsoBI CYCGRG 1 cut(s) 510
Bsp143I GATC 2 cut(s) 172, 506
BspCNI CTCAG 1 cut(s) 412
BspLI GGNNCC 3 cut(s) 284, 456, 457
BspPI GGATC 2 cut(s) 167, 501
BsrI ACTGG 1 cut(s) 231
BssMI GATC 2 cut(s) 172, 506
BstDEI CTNAG 1 cut(s) 420
BstKTI GATC 2 cut(s) 175, 509
BstMAI GTCTC 2 cut(s) 258, 507
BstMBI GATC 2 cut(s) 172, 506
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstNSI RCATGY 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 512
BstX2I RGATCY 2 cut(s) 172, 506
BstYI RGATCY 2 cut(s) 172, 506
BsuI GTATCC 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 2 cut(s) 267, 470
Cfr13I GGNCC 2 cut(s) 229, 455
Csp6I GTAC 1 cut(s) 417
CviAII CATG 3 cut(s) 84, 102, 451
CviJI RGCY 5 cut(s) 47, 114, 310, 338, 474
CviKI_1 RGCY 5 cut(s) 47, 114, 310, 338, 474
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 1 cut(s) 420
DpnI GATC 2 cut(s) 174, 508
DpnII GATC 2 cut(s) 172, 506
Eco47I GGWCC 2 cut(s) 229, 455
Eco88I CYCGRG 1 cut(s) 510
EcoO109I RGGNCCY 1 cut(s) 455
EcoT22I ATGCAT 1 cut(s) 85
Esp3I CGTCTC 1 cut(s) 507
FaeI CATG 3 cut(s) 87, 105, 454
FalI AAGNNNNNCTT 2 cut(s) 160, 192
FaqI GGGAC 1 cut(s) 441
FatI CATG 3 cut(s) 83, 101, 450
FauNDI CATATG 1 cut(s) 496
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 1 cut(s) 501
Fsp4HI GCNGC 1 cut(s) 501
GluI GCNGC 1 cut(s) 501
Hin1II CATG 3 cut(s) 87, 105, 454
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 2 cut(s) 193, 199
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 4 cut(s) 57, 198, 331, 347
Hpy188III TCNNGA 1 cut(s) 510
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 186
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 5 cut(s) 40, 71, 83, 101, 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 3 cut(s) 87, 105, 454
KflI GGGWCCC 1 cut(s) 455
Kzo9I GATC 2 cut(s) 172, 506
LpnPI CCDG 2 cut(s) 212, 312
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 3 cut(s) 49, 70, 418
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 3 cut(s) 88, 211, 247
MalI GATC 2 cut(s) 174, 508
MboI GATC 2 cut(s) 172, 506
MboII GAAGA 1 cut(s) 531
MflI RGATCY 2 cut(s) 172, 506
MluCI AATT 5 cut(s) 26, 66, 241, 332, 528
MlyI GAGTC 1 cut(s) 193
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 1 cut(s) 368
Mph1103I ATGCAT 1 cut(s) 85
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 254
MwoI GCNNNNNNNGC 1 cut(s) 316
NdeI CATATG 1 cut(s) 496
NdeII GATC 2 cut(s) 172, 506
NlaIII CATG 3 cut(s) 87, 105, 454
NlaIV GGNNCC 3 cut(s) 284, 456, 457
NmuCI GTSAC 2 cut(s) 211, 247
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 1 cut(s) 87
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 1 cut(s) 502
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
PpuMI RGGWCCY 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 455
PspN4I GGNNCC 3 cut(s) 284, 456, 457
PspPI GGNCC 2 cut(s) 229, 455
PspPPI RGGWCCY 1 cut(s) 455
PsuI RGATCY 2 cut(s) 172, 506
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
RseI CAYNNNNRTG 1 cut(s) 254
SalI GTCGAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 2 cut(s) 172, 506
Sau96I GGNCC 2 cut(s) 229, 455
SchI GAGTC 1 cut(s) 193
SetI ASST 7 cut(s) 49, 116, 249, 288, 312, 439, 476
SfaNI GCATC 3 cut(s) 49, 70, 418
SfcI CTRYAG 1 cut(s) 115
SinI GGWCC 2 cut(s) 229, 455
SmiMI CAYNNNNRTG 1 cut(s) 254
Sse9I AATT 5 cut(s) 26, 66, 241, 332, 528
TaiI ACGT 1 cut(s) 249
TaqI TCGA 1 cut(s) 108
TasI AATT 5 cut(s) 26, 66, 241, 332, 528
TatI WGTACW 1 cut(s) 416
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 2 cut(s) 274, 470
TseFI GTSAC 2 cut(s) 211, 247
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 2 cut(s) 211, 247
TspDTI ATGAA 4 cut(s) 195, 272, 383, 396
TspGWI ACGGA 2 cut(s) 276, 531
TspRI CASTG 2 cut(s) 274, 470
VpaK11BI GGWCC 2 cut(s) 229, 455
XceI RCATGY 1 cut(s) 87
XmiI GTMKAC 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.