AT5G60040

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003176
Physical Location & Seq
Forward (+)
24173259 .. 24183641
10383 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60040.2

Sequence Viewer

Length: 4131 bp
ATGGAGACGAAGATGGAAATTGAATTCACTAAGAAGCCCTACATCGAAGATGTTGGTCCTCTTAAAATAAAAAGCATAAACTTCTCTGTGCTTTCTGATCTTGAGGTCATGAAAGCCGCTGAAGTTCAAGTCTGGAACATCGGTTTATACGACCACAGTTTTAAACCCTATGAAAACGGCTTGTTGGATCCTCGAATGGGTCCTCCAAACAAAAAGTCTATATGCACAACCTGTGAAGGAAACTTCCAAAATTGTCCGGGACACTATGGATATCTGAAGCTTGACCTCCCAGTTTACAACGTTGGATACTTTAATTTTATCCTTGATATTTTGAAGTGCATTTGTAAGCGCTGTTCCAACATGCTTTTAGATGAGAAATTGTATGAAGATCACTTGAGGAAGATGCGGAATCCCAGAATGGAGCCATTGAAGAAGACAGAATTGGCAAAAGCCGTTGTCAAGAAGTGCAGTACGATGGCGAGCCAGAGAATTATAACGTGCAAAAAATGTGGCTACCTGAATGGGATGGTAAAAAAGATTGCAGCGCAGTTTGGTATAGGCATCAGTCATGACCGATCTAAAATCCATGGTGGAGAGATTGATGAATGTAAATCTGCAATATCCCACACAAAGCAGTCTACTGCAGCAATAAATCCTCTTACCTATGTTCTTGATCCTAACTTGGTGCTCGGGCTTTTTAAAAGAATGAGTGACAAGGACTGCGAACTTCTTTATATTGCCTATAGACCTGAGAATCTTATCATAACGTGCATGCTTGTGCCACCTTTATCCATCCGACCGTCTGTTATGATTGGTGGTATTCAAAGCAATGAAAACGACTTAACAGCGCGATTAAAGCAAATCATTCTAGGCAATGCTTCTCTTCATAAAATTTTAAGTCAACCTACCTCATCCCCCAAAAACATGCAAGTATGGGATACAGTTCAAATCGAGGTTGCAAGATACATTAACAGTGAAGTCCGTGGTTGTCAAAACCAGCCAGAAGAGCATCCACTGTCTGGAATTCTTCAGCGTCTCAAAGGAAAAGGGGGACGTTTCCGTGCAAACTTGTCGGGGAAGCGTGTCGAGTTCACTGGTAGAACTGTAATTTCACCTGATCCCAATCTGAAAATTACAGAGGTAGGAATTCCAATCCTTATGGCTCAGATCCTAACTTTTCCTGAATGTGTTTCTCGTCATAATATTGAGAAGTTGAGGCAATGTGTCCGTAATGGCCCTAATAAATACCCTGGTGCCAGAAATGTCAGATATCCAGATGGTTCTTCAAGGACTTTGGTTGGTGATTATCGCAAGCGTATTGCTGATGAACTGGCCATTGGCTGCATAGTTGACCGCCATTTGCAAGAAGGAGATGTTGTTCTTTTTAACAGGCAACCGAGTCTGCATCGGATGTCTATCATGTGTCACAGGGCAAGAATAATGCCTTGGAGAACGTTGAGGTTCAACGAATCGGTTTGTAACCCATATAATGCTGATTTTGATGGTGATGAGATGAACATGCACGTACCACAAACAGAGGAGGCTCGGACAGAGGCTATTACATTGATGGGGGTACAAAACAATTTATGCACCCCAAAAAATGGAGAAATTTTAGTAGCATCAACGCAGGACTTTTTGACATCTTCTTTTTTGATAACGAGAAAGGACACATTTTATGACCGTGCAGCCTTTTCACTTATATGTTCTTACATGGGAGATGGGATGGATTCCATAGATTTGCCAACACCCACAATCCTTAAGCCAATAGAGCTTTGGACTGGTAAACAGATCTTTAGTGTTTTGCTGCGTCCAAATGCAAGTATTAGAGTCTACGTAACTCTTAATGTGAAAGAGAAGAACTTCAAGAAGGGAGAGCATGGTTTCGATGAAACAATGTGCATAAATGATGGATGGGTTTATTTTCGAAACAGTGAGCTAATATCAGGACAGTTGGGGAAGGCTACGTTAGGAAATGGAAACAAGGATGGATTATATTCTATTCTTCTAAGAGATTACAACTCCCATGCTGCTGCAGTCTGCATGAATCGGCTAGCAAAATTGAGTGCTCGGTGGATTGGTATCCATGGCTTCTCTATTGGAATTGATGACGTTCAACCTGGTGAAGAGTTGAGTAAGGAAAGAAAAGACAGCATCCAGTTTGGATATGACCAGTGCCATCGCAAAATTGAGGAATTTAATAGAGGAAATTTGCAACTTAAAGCTGGTCTAGATGGTGCAAAATCCTTAGAAGCTGAAATAACAGGGATTCTCAATACAATTCGAGAAGCCACTGGGAAGGCCTGTATGAGCGGATTACATTGGAGAAATAGTCCCTTGATCATGTCACAATGTGGTTCCAAAGGATCTCCTATCAATATCAGTCAGATGGTCGCATGTGTTGGTCAGCAGACAGTTAATGGTCACCGTGCTCCTGATGGATTTATAGATCGAAGTCTTCCTCATTTCCCTAGGATGTCCAAATCACCTGCAGCGAAAGGTTTTGTTGCTAATTCGTTCTACAGTGGTCTTACTGCCACAGAATTTTTCTTTCACACTATGGGTGGACGAGAAGGTCTAGTTGATACAGCGGTGAAAACTGCAAGTACAGGTTACATGTCTCGTAGACTGATGAAAGCCTTGGAGGATCTATTAGTCCATTATGATAACACAGTGAGAAATGCCAGCGGATGTATACTTCAATTTACTTATGGGGATGATGGAATGGACCCAGCACTAATGGAAGGAAAGGATGGAGCTCCTTTAAATTTTAATAGATTATTTCTGAAAGTTCAGGCCACATGTCCTCCTCGATCTCACCACACATATCTTTCTTCAGAAGAATTGTCGCAAAAGTTTGAAGAGGAATTAGTCAGACATGATAAAAGTCGGGTTTGCACTGATGCCTTTGTGAAATCTCTTCGAGAATTTGTTTCCTTGCTCGGAGTGAAGTCTGCAAGCCCGCCCCAGGTTTTATATAAAGCATCTGGTGTGACTGATAAGCAACTCGAGGTATTTGTAAAAATTTGTGTATTTCGCTACCGGGAAAAAAAAATTGAGGCTGGGACTGCAATTGGGACAATAGGAGCTCAGAGTATTGGAGAACCTGGGACACAAATGACTTTGAAAACTTTTCACTTTGCTGGAGTTGCGAGCATGAATATCACACAAGGAGTCCCCCGAATCAATGAAATCATCAATGCTTCCAAAAATATAAGCACGCCTGTCATCTCTGCAGAACTTGAGAACCCCCTGGAATTGACTAGTGCACGATGGGTGAAAGGACGCATTGAGAAAACTACTTTAGGACAGGTTGCTGAGAGTATCGAGGTGCTAATGACTTCAACATCAGCGTCAGTGAGGATAATTCTTGACAATAAAATAATCGAGGAGGCATGTTTATCTATAACCCCCTGGTCGGTAAAAAATTCCATCCTAAAGACCCCCAGAATCAAACTCAACGATAATGATATTAGGGTTTTAGATACGGGTTTGGATATTACTCCCGTGGTGGATAAAAGTAGGGCTCATTTCAATCTCCACAATCTGAAGAATGTGCTGCCAAATATTATAGTGAATGGGATCAAAACAGTTGAGCGAGTTGTTGTCGCAGAGGATATGGATAAAAGTAAACAGATAGATGGGAAGACAAAGTGGAAACTGTTTGTGGAGGGCACAAACCTCCTGGCAGTTATGGGGACACCGGGAATCAATGGGAGAACTACTACAAGTAACAATGTTGTCGAAGTAAGCAAAACACTTGGAATCGAGGCTGCAAGGACAACGATTATTGATGAAATAGGGACAGTTATGGGAAACCATGGTATGAGTATAGACATCCGTCACATGATGCTTTTGGCTGATGTCATGACTTACAGGGGGGAGGTTCTCGGAATACAAAGAACCGGGATTCAGAAGATGGATAAAAGTGTTCTGATGCAGGCATCTTTTGAGAGGACTGGAGATCATTTATTTAGTGCAGCAGCTAGCGGAAAAGTTGATAACATAGAGGGAGTTACAGAATGTGTGATTATGGGCATACCAATGAAGCTCGGAACCGGGATACTCAAAGTCCTTCAAAGGACCGATGATTTGCCCAAGTTGAAATATGGACCTGATCCAATCATCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1376

Amino Acids

153.0

Weight (kDa)

8.86

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 22 - 367 1.6e-66 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 369 - 535 6.8e-76 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 538 - 721 6.3e-38 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4 PF05000 746 - 852 1.3e-29 RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5 PF04998 859 - 1325 2.4e-91 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
AarI CACCTGC 1 cut(s) 2494
AasI GACNNNNNNGTC 1 cut(s) 2571
Acc36I ACCTGC 1 cut(s) 2494
AccB1I GGYRCC 1 cut(s) 1253
AccB7I CCANNNNNTGG 2 cut(s) 1019, 1601
AccBSI CCGCTC 1 cut(s) 2310
AccI GTMKAC 4 cut(s) 638, 1830, 2623, 2692
AccII CGCG 1 cut(s) 850
AciI CCGC 8 cut(s) 117, 406, 1354, 2310, 2588, 2685, 2960, 3987
AclI AACGTT 2 cut(s) 300, 1454
AclWI GGATC 9 cut(s) 182, 195, 668, 1112, 1162, 2371, 2652, 3587, 4109
AcoI YGGCCR 1 cut(s) 1332
AcuI CTGAAG 5 cut(s) 141, 296, 1013, 2817, 3566
AdeI CACNNNGTG 1 cut(s) 2351
AfaI GTAC 4 cut(s) 472, 1527, 1575, 2605
AfeI AGCGCT 1 cut(s) 350
AfiI CCNNNNNNNGG 5 cut(s) 197, 1019, 1601, 2965, 3415
AflII CTTAAG 1 cut(s) 1757
AflIII ACRYGT 2 cut(s) 2613, 2798
AhlI ACTAGT 1 cut(s) 3260
AjnI CCWGG 7 cut(s) 1249, 2116, 2964, 3103, 3249, 3410, 3681
AjuI GAANNNNNNNTTGG 6 cut(s) 425, 457, 1320, 1352, 1429, 1461
AloI GAACNNNNNNTCC 2 cut(s) 1362, 1394
AluBI AGCT 9 cut(s) 280, 1771, 1936, 2222, 2252, 2756, 3086, 3983, 4048
AluI AGCT 9 cut(s) 280, 1771, 1936, 2222, 2252, 2756, 3086, 3983, 4048
Alw21I GWGCWC 6 cut(s) 690, 2068, 2431, 2758, 3088, 3268
Alw26I GTCTC 2 cut(s) 1040, 2622
Alw44I GTGCAC 1 cut(s) 3264
AlwI GGATC 9 cut(s) 182, 195, 668, 1112, 1162, 2371, 2652, 3587, 4109
Ama87I CYCGRG 2 cut(s) 689, 3005
Aor51HI AGCGCT 1 cut(s) 350
AoxI GGCC 4 cut(s) 1234, 1332, 2298, 2793
ApaLI GTGCAC 1 cut(s) 3264
ArsI GACNNNNNNTTYG 4 cut(s) 200, 232, 4072, 4104
Asp700I GAANNNNTTC 1 cut(s) 1859
AspA2I CCTAGG 1 cut(s) 2468
AspLEI GCGC 3 cut(s) 351, 547, 850
AspS9I GGNCC 6 cut(s) 56, 200, 1235, 2725, 4080, 4109
AsuC2I CCSGG 5 cut(s) 258, 3041, 3702, 3904, 4057
AsuHPI GGTGA 9 cut(s) 1104, 1313, 1517, 2132, 2414, 2475, 2602, 2807, 3286
AsuII TTCGAA 1 cut(s) 1924
AsuNHI GCTAGC 2 cut(s) 2050, 3983
AvaI CYCGRG 2 cut(s) 689, 3005
AvaII GGWCC 5 cut(s) 56, 200, 2725, 4080, 4109
AvrII CCTAGG 1 cut(s) 2468
BaeGI GKGCMC 2 cut(s) 3268, 3674
BaeI ACNNNNGTAYC 2 cut(s) 2574, 2607
BalI TGGCCA 1 cut(s) 1334
BamHI GGATCC 1 cut(s) 187
BanI GGYRCC 1 cut(s) 1253
BanII GRGCYC 3 cut(s) 2758, 3088, 3526
BbsI GAAGAC 3 cut(s) 440, 2447, 3650
Bbv12I GWGCWC 6 cut(s) 690, 2068, 2431, 2758, 3088, 3268
BceAI ACGGC 2 cut(s) 193, 437
BcgI CGANNNNNNTGC 2 cut(s) 1053, 1087
BciT130I CCWGG 7 cut(s) 1251, 2118, 2966, 3105, 3251, 3412, 3683
BciVI GTATCC 4 cut(s) 299, 931, 2090, 4053
BclI TGATCA 1 cut(s) 2337
BcnI CCSGG 5 cut(s) 258, 3041, 3702, 3904, 4057
BcoDI GTCTC 2 cut(s) 1040, 2622
BcuI ACTAGT 1 cut(s) 3260
BfaI CTAG 7 cut(s) 869, 2051, 2228, 2469, 2576, 3261, 3984
BfmI CTRYAG 6 cut(s) 642, 742, 2031, 2487, 2518, 3231
BfoI RGCGCY 1 cut(s) 352
BfrI CTTAAG 1 cut(s) 1757
BfuAI ACCTGC 1 cut(s) 2494
BfuI GTATCC 4 cut(s) 299, 931, 2090, 4053
BglII AGATCT 1 cut(s) 1788
BlnI CCTAGG 1 cut(s) 2468
Bme18I GGWCC 5 cut(s) 56, 200, 2725, 4080, 4109
BmeT110I CYCGRG 2 cut(s) 689, 3005
BmgT120I GGNCC 6 cut(s) 56, 200, 1235, 2725, 4080, 4109
BmiI GGNNCC 7 cut(s) 189, 201, 423, 1255, 2356, 2727, 4054
BmrI ACTGGG 2 cut(s) 284, 2301
BmtI GCTAGC 2 cut(s) 2054, 3987
BmuI ACTGGG 2 cut(s) 284, 2301
BoxI GACNNNNGTC 1 cut(s) 3837
BpiI GAAGAC 3 cut(s) 440, 2447, 3650
BpmI CTGGAG 2 cut(s) 3162, 3978
Bpu14I TTCGAA 1 cut(s) 1924
BpuEI CTTGAG 3 cut(s) 122, 415, 3260
BpuMI CCSGG 5 cut(s) 258, 3041, 3702, 3904, 4057
BsaAI YACGTR 2 cut(s) 1525, 1834
BsaBI GATNNNNATC 3 cut(s) 1122, 1415, 2078
BsaXI ACNNNNNCTCC 6 cut(s) 1362, 1392, 2314, 2344, 2788, 2818
Bsc4I CCNNNNNNNGG 5 cut(s) 197, 1019, 1601, 2965, 3415
Bse1I ACTGG 8 cut(s) 290, 1099, 1335, 1783, 2155, 2170, 2296, 3961
Bse3DI GCAATG 3 cut(s) 835, 880, 1226
Bse8I GATNNNNATC 3 cut(s) 1122, 1415, 2078
BseBI CCWGG 7 cut(s) 1251, 2118, 2966, 3105, 3251, 3412, 3683
BseJI GATNNNNATC 3 cut(s) 1122, 1415, 2078
BseLI CCNNNNNNNGG 5 cut(s) 197, 1019, 1601, 2965, 3415
BseMI GCAATG 3 cut(s) 835, 880, 1226
BseMII CTCAG 4 cut(s) 741, 1178, 3101, 3306
BseNI ACTGG 8 cut(s) 290, 1099, 1335, 1783, 2155, 2170, 2296, 3961
BseRI GAGGAG 3 cut(s) 1553, 2796, 3401
BseSI GKGCMC 2 cut(s) 3268, 3674
BseYI CCCAGC 2 cut(s) 2728, 3059
BsgI GTGCAG 3 cut(s) 487, 1704, 3996
Bsh1236I CGCG 1 cut(s) 850
Bsh1285I CGRYCG 1 cut(s) 800
BshFI GGCC 4 cut(s) 1236, 1334, 2300, 2795
BshNI GGYRCC 1 cut(s) 1253
BsiEI CGRYCG 1 cut(s) 800
BsiHKAI GWGCWC 6 cut(s) 690, 2068, 2431, 2758, 3088, 3268
BsiHKCI CYCGRG 2 cut(s) 689, 3005
BsiSI CCGG 5 cut(s) 257, 3040, 3701, 3903, 4056
BslFI GGGAC 9 cut(s) 273, 1065, 2316, 3076, 3088, 3121, 3158, 3709, 3814
BslI CCNNNNNNNGG 5 cut(s) 197, 1019, 1601, 2965, 3415
BsmAI GTCTC 2 cut(s) 1040, 2622
BsmBI CGTCTC 1 cut(s) 1040
BsmFI GGGAC 9 cut(s) 273, 1065, 2316, 3076, 3088, 3121, 3158, 3709, 3814
BsnI GGCC 4 cut(s) 1236, 1334, 2300, 2795
BsoBI CYCGRG 2 cut(s) 689, 3005
Bsp119I TTCGAA 1 cut(s) 1924
Bsp1286I GDGCHC 8 cut(s) 690, 2068, 2431, 2758, 3088, 3268, 3526, 3674
Bsp19I CCATGG 3 cut(s) 586, 2083, 3817
BspACI CCGC 8 cut(s) 117, 406, 1354, 2310, 2588, 2685, 2960, 3987
BspANI GGCC 4 cut(s) 1236, 1334, 2300, 2795
BspCNI CTCAG 4 cut(s) 742, 1177, 3100, 3307
BspFNI CGCG 1 cut(s) 850
BspHI TCATGA 3 cut(s) 108, 568, 3864
BspLI GGNNCC 7 cut(s) 189, 201, 423, 1255, 2356, 2727, 4054
BspMAI CTGCAG 4 cut(s) 646, 2035, 2491, 3235
BspMI ACCTGC 1 cut(s) 2494
BspOI GCTAGC 2 cut(s) 2054, 3987
BspPI GGATC 9 cut(s) 182, 195, 668, 1112, 1162, 2371, 2652, 3587, 4109
BspQI GCTCTTC 1 cut(s) 999
BspT104I TTCGAA 1 cut(s) 1924
BspT107I GGYRCC 1 cut(s) 1253
BspTI CTTAAG 1 cut(s) 1757
BsrBI CCGCTC 1 cut(s) 2310
BsrDI GCAATG 3 cut(s) 835, 880, 1226
BsrI ACTGG 8 cut(s) 290, 1099, 1335, 1783, 2155, 2170, 2296, 3961
BssNAI GTATAC 1 cut(s) 2693
BssT1I CCWWGG 6 cut(s) 586, 1445, 2083, 2468, 2637, 3817
Bst1107I GTATAC 1 cut(s) 2693
Bst2UI CCWGG 7 cut(s) 1251, 2118, 2966, 3105, 3251, 3412, 3683
Bst6I CTCTTC 5 cut(s) 888, 999, 2118, 2853, 2922
BstAFI CTTAAG 1 cut(s) 1757
BstBAI YACGTR 2 cut(s) 1525, 1834
BstBI TTCGAA 1 cut(s) 1924
BstDEI CTNAG 7 cut(s) 30, 750, 1164, 2006, 2245, 3087, 3315
BstDSI CCRYGG 5 cut(s) 586, 982, 2083, 3504, 3817
BstEII GGTNACC 1 cut(s) 2420
BstFNI CGCG 1 cut(s) 850
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 3 cut(s) 351, 547, 850
BstMAI GTCTC 2 cut(s) 1040, 2622
BstMCI CGRYCG 1 cut(s) 800
BstMWI GCNNNNNNNGC 5 cut(s) 856, 1006, 1768, 3065, 3146
BstNI CCWGG 7 cut(s) 1251, 2118, 2966, 3105, 3251, 3412, 3683
BstNSI RCATGY 8 cut(s) 364, 775, 928, 1522, 2397, 2617, 2802, 3396
BstPAI GACNNNNGTC 1 cut(s) 3837
BstPI GGTNACC 1 cut(s) 2420
BstSFI CTRYAG 6 cut(s) 642, 742, 2031, 2487, 2518, 3231
BstSLI GKGCMC 2 cut(s) 3268, 3674
BstSNI TACGTA 1 cut(s) 1834
BstUI CGCG 1 cut(s) 850
BstV2I GAAGAC 3 cut(s) 440, 2447, 3650
BstX2I RGATCY 5 cut(s) 187, 1167, 1788, 2363, 2644
BstYI RGATCY 5 cut(s) 187, 1167, 1788, 2363, 2644
BstZ17I GTATAC 1 cut(s) 2693
BsuI GTATCC 4 cut(s) 299, 931, 2090, 4053
BsuRI GGCC 4 cut(s) 1236, 1334, 2300, 2795
BtgI CCRYGG 5 cut(s) 586, 982, 2083, 3504, 3817
BtgZI GCGATG 1 cut(s) 2162
BveI ACCTGC 1 cut(s) 2494
CciI TCATGA 3 cut(s) 108, 568, 3864
CfoI GCGC 3 cut(s) 351, 547, 850
Cfr13I GGNCC 6 cut(s) 56, 200, 1235, 2725, 4080, 4109
CseI GACGC 4 cut(s) 1022, 1796, 3291, 3339
CsiI ACCWGGT 1 cut(s) 2116
Csp6I GTAC 4 cut(s) 471, 1526, 1574, 2604
CspCI CAANNNNNGTGG 2 cut(s) 490, 525
CviQI GTAC 4 cut(s) 471, 1526, 1574, 2604
DdeI CTNAG 7 cut(s) 30, 750, 1164, 2006, 2245, 3087, 3315
DraI TTTAAA 3 cut(s) 163, 700, 2763
DraIII CACNNNGTG 1 cut(s) 2351
DrdI GACNNNNNNGTC 1 cut(s) 2571
DseDI GACNNNNNNGTC 1 cut(s) 2571
EaeI YGGCCR 1 cut(s) 1332
Eam1104I CTCTTC 5 cut(s) 888, 999, 2118, 2853, 2922
EarI CTCTTC 5 cut(s) 888, 999, 2118, 2853, 2922
Ecl136II GAGCTC 2 cut(s) 2756, 3086
Eco105I TACGTA 1 cut(s) 1834
Eco130I CCWWGG 6 cut(s) 586, 1445, 2083, 2468, 2637, 3817
Eco147I AGGCCT 1 cut(s) 2300
Eco24I GRGCYC 3 cut(s) 2758, 3088, 3526
Eco32I GATATC 2 cut(s) 272, 1271
Eco47I GGWCC 5 cut(s) 56, 200, 2725, 4080, 4109
Eco47III AGCGCT 1 cut(s) 350
Eco53kI GAGCTC 2 cut(s) 2756, 3086
Eco57I CTGAAG 5 cut(s) 141, 296, 1013, 2817, 3566
Eco88I CYCGRG 2 cut(s) 689, 3005
Eco91I GGTNACC 1 cut(s) 2420
EcoICRI GAGCTC 2 cut(s) 2756, 3086
EcoO109I RGGNCCY 1 cut(s) 200
EcoO65I GGTNACC 1 cut(s) 2420
EcoRI GAATTC 3 cut(s) 23, 1023, 1146
EcoRII CCWGG 7 cut(s) 1249, 2116, 2964, 3103, 3249, 3410, 3681
EcoRV GATATC 2 cut(s) 272, 1271
EcoT14I CCWWGG 6 cut(s) 586, 1445, 2083, 2468, 2637, 3817
EcoT38I GRGCYC 3 cut(s) 2758, 3088, 3526
ErhI CCWWGG 6 cut(s) 586, 1445, 2083, 2468, 2637, 3817
Esp3I CGTCTC 1 cut(s) 1040
FaqI GGGAC 9 cut(s) 273, 1065, 2316, 3076, 3088, 3121, 3158, 3709, 3814
FauI CCCGC 1 cut(s) 2967
FbaI TGATCA 1 cut(s) 2337
FblI GTMKAC 4 cut(s) 638, 1830, 2623, 2692
FriOI GRGCYC 3 cut(s) 2758, 3088, 3526
FspBI CTAG 7 cut(s) 869, 2051, 2228, 2469, 2576, 3261, 3984
GlaI GCGC 3 cut(s) 350, 546, 849
GsaI CCCAGC 2 cut(s) 2732, 3063
GsuI CTGGAG 2 cut(s) 3162, 3978
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 4 cut(s) 1236, 1334, 2300, 2795
HapII CCGG 5 cut(s) 257, 3040, 3701, 3903, 4056
HgaI GACGC 4 cut(s) 1022, 1796, 3291, 3339
HhaI GCGC 3 cut(s) 351, 547, 850
Hin6I GCGC 3 cut(s) 349, 545, 848
HinP1I GCGC 3 cut(s) 349, 545, 848
HincII GTYRAC 2 cut(s) 902, 1351
HindII GTYRAC 2 cut(s) 902, 1351
HindIII AAGCTT 1 cut(s) 278
HpaII CCGG 5 cut(s) 257, 3040, 3701, 3903, 4056
HphI GGTGA 9 cut(s) 1104, 1313, 1517, 2132, 2414, 2475, 2602, 2807, 3286
HpyAV CCTTC 8 cut(s) 230, 1361, 1861, 1951, 2290, 2564, 2735, 4082
HpyCH4IV ACGT 9 cut(s) 300, 497, 767, 1054, 1454, 1524, 1833, 1964, 2109
HpyF10VI GCNNNNNNNGC 5 cut(s) 856, 1006, 1768, 3065, 3146
HpyF3I CTNAG 7 cut(s) 30, 750, 1164, 2006, 2245, 3087, 3315
HpySE526I ACGT 9 cut(s) 300, 497, 767, 1054, 1454, 1524, 1833, 1964, 2109
HspAI GCGC 3 cut(s) 349, 545, 848
Ksp22I TGATCA 1 cut(s) 2337
LguI GCTCTTC 1 cut(s) 999
LmnI GCTCC 5 cut(s) 421, 2434, 2753, 2761, 3083
MabI ACCWGGT 1 cut(s) 2116
MaeI CTAG 7 cut(s) 869, 2051, 2228, 2469, 2576, 3261, 3984
MaeII ACGT 9 cut(s) 300, 497, 767, 1054, 1454, 1524, 1833, 1964, 2109
MbiI CCGCTC 1 cut(s) 2310
MfeI CAATTG 1 cut(s) 3069
MflI RGATCY 5 cut(s) 187, 1167, 1788, 2363, 2644
MhlI GDGCHC 8 cut(s) 690, 2068, 2431, 2758, 3088, 3268, 3526, 3674
MlsI TGGCCA 1 cut(s) 1334
MluNI TGGCCA 1 cut(s) 1334
MlyI GAGTC 3 cut(s) 1408, 1836, 3180
MmeI TCCRAC 4 cut(s) 165, 283, 381, 820
Mox20I TGGCCA 1 cut(s) 1334
MroXI GAANNNNTTC 1 cut(s) 1859
MscI TGGCCA 1 cut(s) 1334
MslI CAYNNNNRTG 3 cut(s) 776, 1699, 4040
Msp20I TGGCCA 1 cut(s) 1334
MspA1I CMGCKG 3 cut(s) 119, 2588, 2685
MspCI CTTAAG 1 cut(s) 1757
MspI CCGG 5 cut(s) 257, 3040, 3701, 3903, 4056
MunI CAATTG 1 cut(s) 3069
MvaI CCWGG 7 cut(s) 1251, 2118, 2966, 3105, 3251, 3412, 3683
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 5 cut(s) 856, 1006, 1768, 3065, 3146
NciI CCSGG 5 cut(s) 258, 3041, 3702, 3904, 4057
NcoI CCATGG 3 cut(s) 586, 2083, 3817
NheI GCTAGC 2 cut(s) 2050, 3983
NlaIV GGNNCC 7 cut(s) 189, 201, 423, 1255, 2356, 2727, 4054
NmuCI GTSAC 6 cut(s) 710, 1424, 2343, 2420, 2989, 3839
NspI RCATGY 8 cut(s) 364, 775, 928, 1522, 2397, 2617, 2802, 3396
NspV TTCGAA 1 cut(s) 1924
PaeI GCATGC 1 cut(s) 775
PaeR7I CTCGAG 1 cut(s) 3005
PagI TCATGA 3 cut(s) 108, 568, 3864
PaqCI CACCTGC 1 cut(s) 2494
PceI AGGCCT 1 cut(s) 2300
PciI ACATGT 2 cut(s) 2613, 2798
PciSI GCTCTTC 1 cut(s) 999
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 1859
PflMI CCANNNNNTGG 2 cut(s) 1019, 1601
PfoI TCCNGGA 1 cut(s) 256
PleI GAGTC 3 cut(s) 1407, 1835, 3179
PpsI GAGTC 3 cut(s) 1407, 1835, 3179
Ppu21I YACGTR 2 cut(s) 1525, 1834
PpuMI RGGWCCY 1 cut(s) 200
PscI ACATGT 2 cut(s) 2613, 2798
PshAI GACNNNNGTC 1 cut(s) 3837
PsiI TTATAA 1 cut(s) 494
Psp124BI GAGCTC 2 cut(s) 2758, 3088
Psp1406I AACGTT 2 cut(s) 300, 1454
Psp5II RGGWCCY 1 cut(s) 200
Psp6I CCWGG 7 cut(s) 1249, 2116, 2964, 3103, 3249, 3410, 3681
PspEI GGTNACC 1 cut(s) 2420
PspFI CCCAGC 2 cut(s) 2728, 3059
PspGI CCWGG 7 cut(s) 1249, 2116, 2964, 3103, 3249, 3410, 3681
PspN4I GGNNCC 7 cut(s) 189, 201, 423, 1255, 2356, 2727, 4054
PspPI GGNCC 6 cut(s) 56, 200, 1235, 2725, 4080, 4109
PspPPI RGGWCCY 1 cut(s) 200
PspXI VCTCGAGB 1 cut(s) 3005
PsrI GAACNNNNNNTAC 2 cut(s) 337, 369
PstI CTGCAG 4 cut(s) 646, 2035, 2491, 3235
PsuI RGATCY 5 cut(s) 187, 1167, 1788, 2363, 2644
RsaI GTAC 4 cut(s) 472, 1527, 1575, 2605
RsaNI GTAC 4 cut(s) 471, 1526, 1574, 2604
RseI CAYNNNNRTG 3 cut(s) 776, 1699, 4040
SacI GAGCTC 2 cut(s) 2758, 3088
SapI GCTCTTC 1 cut(s) 999
Sau96I GGNCC 6 cut(s) 56, 200, 1235, 2725, 4080, 4109
SchI GAGTC 3 cut(s) 1408, 1836, 3180
SduI GDGCHC 8 cut(s) 690, 2068, 2431, 2758, 3088, 3268, 3526, 3674
SexAI ACCWGGT 1 cut(s) 2116
SfcI CTRYAG 6 cut(s) 642, 742, 2031, 2487, 2518, 3231
Sfr274I CTCGAG 1 cut(s) 3005
SfuI TTCGAA 1 cut(s) 1924
SinI GGWCC 5 cut(s) 56, 200, 2725, 4080, 4109
SlaI CTCGAG 1 cut(s) 3005
SmiMI CAYNNNNRTG 3 cut(s) 776, 1699, 4040
SmlI CTYRAG 5 cut(s) 101, 394, 1757, 3005, 3239
SmoI CTYRAG 5 cut(s) 101, 394, 1757, 3005, 3239
SnaBI TACGTA 1 cut(s) 1834
SpeI ACTAGT 1 cut(s) 3260
SphI GCATGC 1 cut(s) 775
SseBI AGGCCT 1 cut(s) 2300
SsiI CCGC 8 cut(s) 117, 406, 1354, 2310, 2588, 2685, 2960, 3987
SspI AATATT 2 cut(s) 1204, 3565
SspMI CTAG 7 cut(s) 869, 2051, 2228, 2469, 2576, 3261, 3984
SstI GAGCTC 2 cut(s) 2758, 3088
StuI AGGCCT 1 cut(s) 2300
StyI CCWWGG 6 cut(s) 586, 1445, 2083, 2468, 2637, 3817
TaiI ACGT 9 cut(s) 303, 500, 770, 1057, 1457, 1527, 1836, 1967, 2112
TaqII GACCGA 2 cut(s) 588, 4097
TatI WGTACW 1 cut(s) 2603
TauI GCSGC 1 cut(s) 119
TseFI GTSAC 6 cut(s) 710, 1424, 2343, 2420, 2989, 3839
Tsp45I GTSAC 6 cut(s) 710, 1424, 2343, 2420, 2989, 3839
TspGWI ACGGA 4 cut(s) 971, 1049, 1217, 3827
Van91I CCANNNNNTGG 2 cut(s) 1019, 1601
Vha464I CTTAAG 1 cut(s) 1757
VneI GTGCAC 1 cut(s) 3264
VpaK11BI GGWCC 5 cut(s) 56, 200, 2725, 4080, 4109
XbaI TCTAGA 1 cut(s) 2227
XceI RCATGY 8 cut(s) 364, 775, 928, 1522, 2397, 2617, 2802, 3396
XcmI CCANNNNNNNNNTGG 1 cut(s) 1770
XhoI CTCGAG 1 cut(s) 3005
XmaJI CCTAGG 1 cut(s) 2468
XmiI GTMKAC 4 cut(s) 638, 1830, 2623, 2692
XmnI GAANNNNTTC 1 cut(s) 1859
XspI CTAG 7 cut(s) 869, 2051, 2228, 2469, 2576, 3261, 3984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.