Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0003176

Physical Location & Seq

Genomic Coordinates Reverse (-)

69113714 .. 69144325

Gene Structure 30612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG432400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4605 bp

ATGATGGAGTCGGGGCAGGACCCGAAGCTGCAGTTGTTGATCCGGGCGACGTTGGGGTCGTGGTGGGAAAATTCGGCGACTATTTCGGAGGGAGTGATGAAAGGAGAGTTTGGATTCCCGTTAAGGGTGTGGTTGCGGGAGGAGGTTGTGGTCGGGACAGTCGTCGAGGGCGTTGAGGACGGAGTGGGTTTGGGCAACGTCGAAGATGGCACGGGTCATGGCGGCGATGACTTTGGGGTCGGAGAGGTAAGGCATCTGGCTAAGGAGGATGAGGATGGTTGCTGGGACTGGAGTCCGCTGAAGAAGACACAAGGGTACACTCCCGCCCTTTACACCAAACGCAGCCCCTTCAACCTTCAAACCTCCCTCTTTGCTTTCTGCGTGGAGAAGAGACTTCTACCGGATTTTCTCTCTTCAATTTTGATAGAAAAGATGATGAATAAAGTCCAAGCCAAAACATTAGCCTTCACAAAAGAGCCTTTCATTGAAGAAGAAGGGCACTGCAGAATAGCACAATGGCAATTCAATGCTCTGTCCCAGTCAGAGATCAGCAAAATGGCTGCAACTCAAATTTTTCTGGGTCGATATTACGATAGCAATCAAAAACCAATCAAAGGAGGCTTGTTGGATCCCCGCTTGGGCCCTGCAAATAGGCAATGCGGTGACTGTGAGACATGTGGTGCGAAACTTTCAGATTGTCCTGGCCACTTTGGATATTTGGTGCTTGCTGTCCCTGTATTCAATGTTGGATATTTAAGTACTGTTGTGGACGTTTTGAAGTGCATTTGTAAGTCATGCTCTCGAATAATTTTGGATGAAAAATCACGCAGTCACTTTTTGAATATTGTGAGAGATCCCAAAAAGGATAAAAGCGTGACAATGAAATCAATAGTGAAGAAGTGTACTAGCATGGCAAGCAATAATAGATCAGTGAAGTGCCCGAAATGTGGATATATGAATGGTTCAGTGAAAAGGGCTCAAGGTATGCTGGCAATTGTTCATGGTGCTGTGGATGACTTTAAATCAGCGAGTCCTGTCATGAAGGATTCCAAGGCATCCATTAATCTCCAACCTCATATGCTTAACCCTGTTAAAGTTTTTTCTTTGTTCAAAAGAATGCTTGATGAGGATTGTGAACTTCTTAATCTTAAAAGTAGACCAGAGAACCTCATCATGACAACCATTGCTGTGCCACCTATAGCCATTCGTCCTTCCGTTCTTGTGGATGGATCACAGAGTAACGAAAATGACATTACAGAGAGGTTGAAGAAAATTATTCAAACAAACGCTAATGTTCAACTGGACCTATCAGAAGGTTCTACCACAAGATTTTTGTCAAATTGGGACGTACTTCAAGCCGAAGTTGCGCTGTACATAAACAGTGATGTTCGCGGTATTCCTTATCACGTGCAACTAAATACCAAGCCATTGAATGGTTTGGTACAGCGCCTCAAAGGGAAGACGGGCCGTTTTCGTGGAAACCTATGTGGGAAAAGGGTTGAATATACTGGCAGAACTGTTATTTCACCCGACCCTAATCTGAAAATTACTGAGGTCGGAATCCCTATCCAAATGGCTCAGATTTTAAGTTATCCAGAACGTGTGTCCCATCATAATATTGAAAAGTTGAAGCAGTGTGTATTTAATGGACCTTCTAAGTACCCTGGTGCCCTAGTGCTCAAACATCAAAATGGCGAGGAATGGTCCTTGAAGGCTGAGCGACAAAGGAAGTCTCATGCTGATAATCTCCAATATGGTGACATAGTTGATCGCCACTTAGAAGATGGGGACATTGTTCTTTTCAACAGACAACCAAGCTTGCATCGGATGTCCATCATGTGTCATAGGGCAAGGATTATGCCTTGGAGAACATTGAGGTTTAATGAATCTGTATGCAACCCTTATAATGCTGATTTTGATGGTGACGAAATGAACATGCATGTGCCGCAAACAGAGGAGGCTCGAACTGAGGCTGTTATGTTGATGGGGGTGCAAAACAATCTATGCACTCCCAAAAATGGGGAGATTTTGGTTGCTTCCACTCAAGATTTTCTAACATCTTCTTTCCTTATAACAAGGAAGGACACATTTTATGACCGTGCATCTTTTTCACTCATGTGTTCGTACATGGGGGATGGCACGGAGACTGTGGATTTGCCAACACCTGCTGTAATTAAGCCAATAGAGCTATGGACAGGAAAGCAGCTATATAGTGTGTTAGTTCGCCCAAATTCAAATGTGAGAGTATATCTAAACCTAACTGTTAGAGAGAAATCCTACTCCAACACAAAAACTGAAGATGGAAGAGAAACAGAAGCAATGTGCCCAAACGATGGGTTTGTCTATTTCCGTAACAGTGAGCTATTATCTGGGCAACTTGGGAAGGGCACTTTAGGAAATGGCAATAAGGATGGACTTTTCTCTGTTCTTCTCAGAGACTATAAAGCTCATGCTGCTGCTTCTTGCATGAATCGTGTAGCTAAATTGAGTGCCCGGTGGATTGGTAATCACGGATTCTCAATTGGGATTAGTGATGTACAACCTAGTGACATCTTGTACGGTGAGAAAGAAAAAATCATTAAGAAAGGTTATGACAAATGTGACGACCAGATAAAGTTGTATAAAGAAGGACAGCTACGACTTGCACCTGGTTGTGATGCTGCCCAATCACTTGAATCTGGGATTACTGCAGTATTAAACGATATTAGGGACCAAACTGGAAAGCTGTGCATGCAAACGCTACATTGGAGAAACAGTCCCCTGATTATGTCCCAGTGTGGTTCCAAAGGATCTGCTATCAATATCAGCCAGATGGTTGCTTGTGTTGGTCAACAATCAGTTGGTGGTCGTCGTGCACCAAATGGTTTCATAGATCGAAGCCTTCCTCACTTTCCTAGAAAAGAAAATACCCCTGCAGCTAAAGGCTTTGTTGCTAGTTCCTTTTACAGTGGCTTGACAGCTACAGAGTTCTTTTTTCATACAATGGGAGGGCGAGAAGGCCTTGTGGATACAGCTGTAAAAACTGCTGACACAGGGTACATGTCTCGTAGATTGTCTAAAGTTCTAGAGGACCTCTCTGTCCAGTATGATAAAACAGTGCGGGCCGCAAATGGAGGTATTGTTCAGTTTTGTTATGGGGATGACGGTATGGATCCTGCCATGATGGAAGGAAAAAGTGGAGCTCCGTTAGACTTCTACCGATTGTTTTTCAAAGCTAAGGCCACATGCCCTGCTGAAGAAAGTAAAAGCCTTTCTCCTGATGAAGTGTCTGAAATAGTGAGAAGCAGGCTTTCGGAACAAGATATGACTCCGGATGGTGGTTGCTCTATGGGTTTTAAAAGTTCTATAGAGCAATTCCTGGATGAATATGTAAAAGCATTAAGAAAAACACAGGAGGCTTTTTTATTGAATCAAAGTCCTGGTTGGAAGGTGAAGTCTGCAACTGTAGAGAAGATTGTCCAAAATATATCTGGTGTCACTTCTAGACAGCTGAAGGTTTTTCTAGATACTTGTATTTCTCGTTATCATTCAAAAAAAGTTGATGCGGGAACAGCAATTGGAGTAATTGGAGCTCAAAGCATTGGCGAGCCTGGGACACAGATGACATTAAAAACTTTTCACTTTGCTGGAGTTGCAAGCATGAATGTTACACTTGGAGTCCCTCGTATCAAGGAAATAATAAATGGAGCAAAAAATATCCATACCCCTATCATCACTACAGTACTTGAGTGTGATAATAATCCGATGATTGCACGGATGGTAGCAGGTCGGATTGAAAAAACAAATCTAGGGCAGGTTGCTGAGTGTATAGAGATTGTAATGAGTCCAAGAGCAGCATATGTAGCCATCAAGCTTGATATGTTTGTGATACAAGATGCACACTTGTCTATAGATGCCAATGTTGTAAAACAATCAATTTTGGGAACTCCAAGGATTAAACTGAAACATGAGCACATTAGGGTTCCAGATAATCAAACAGTTGAAGTTCATTCTCACGAGAAGGATAGAAGAAAACTCCATTTTCACCTCTTCTATTTGAAGAGTATCCTCCCCAGTGTTATAGTAAGGGGTATAAAGACAATTGAACGTGTTGTCATTGATGAAGAAAAAGTTAAAAGGGACAAAAAAGATATTGACGTTAAATGTGGAGAAGAAAAGAAGTACAAGTTATTTGCTGAAGGCAAGGGTCTTCTGGCAGTAATGAGCACTAAAGGAGTAGATGGTTGTAAAACCACAAGTAATGATATTATTGAAGTGCAGCAGACGCTTGGAATTGAAGCTGCAAGAACTTGTATCATTGAGGAGATAAAGTATACTATGAAATCACACGGGATGAACATAGATGATCGTCATATGATGCTTTTAGCGGATGTAATGACATTCAGAGGTTTAGTTCTTGGGATCACAAGATTCGGCGTTCAAAAGATGGGCAAAAGTGTATTGATGCTGGCTTCATTTGAGCGAACGGATGATCATCTATTTAATGCTTGCGTTCAGGGGACAGTTGACAAAATTGAAGGAGTAAGTGAAAGCATAATCATGGGTATACCAATGCCAATAGGCACCGGAATGTTCAAAATTAAACAACGCCTCCCTGCAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002376 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0046483 GO:0048518 GO:0050789 GO:0050896 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1534

Amino Acids

170.36

Weight (kDa)

8.66

Isoelectric Point (pI)

38.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	169 - 495	6.3e-76	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	497 - 664	3.4e-74	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	667 - 846	7.5e-38	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	874 - 977	4.5e-29	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	984 - 1479	1.6e-88	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60040 AT5G60040
fragaria_vesca	FvH4_3g34290 FvH4_3g34290 FvH4_3g34290 FvH4_6g15631
malus_domestica	MD03G1106800.v1.1 MD09G1157000.v1.1
prunus_persica	Prupe.6G089600_v2.0.a1 Prupe.6G089600_v2.0.a1
pyrus_communis	pycom03g08620 pycom09g07570
rosa_chinensis	RchiOBHm_Chr3g0469301 RchiOBHm_Chr3g0469321 RchiOBHm_Chr5g0061861
rosa_laevigata	RLG00000024360 RLG00000035511
rosa_multiflora	Rmu_sc0004134.1_g000001 Rmu_sc0011641.1_g000013 Rmu_sc0011641.1_g000015 Rmu_sc0019271.1_g000001 Rmu_sc0022191.1_g000001
rosa_roxburghii	Rroxscaffold_1G00018870 Rroxscaffold_6G00411990 Rroxscaffold_6G00412000
rosa_rugosa	Rorug03G0100400 Rorug05G0344900 Rorug05G0345000
rosa_samantha	Rh3AG149800 Rh3BG173400 Rh3CG164500 Rh3DG202000 Rh5AG405500 Rh5BG418300 Rh5CG442800 Rh5DG432400
rosa_wichuraiana	Rw5G038070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1905, 2072
AarI	CACCTGC	1 cut(s)	2173
AasI	GACNNNNNNGTC	3 cut(s)	55, 236, 3077
Acc36I	ACCTGC	3 cut(s)	2173, 3755, 3784
AccB1I	GGYRCC	2 cut(s)	1667, 4562
AccB7I	CCANNNNNTGG	2 cut(s)	1433, 2333
AccI	GTMKAC	3 cut(s)	1156, 4313, 4546
AccII	CGCG	1 cut(s)	1392
AccIII	TCCGGA	1 cut(s)	3310
AclWI	GGATC	9 cut(s)	34, 623, 636, 848, 1237, 2797, 3146, 3159, 4409
AcoI	YGGCCR	1 cut(s)	703
AcsI	RAATTY	3 cut(s)	70, 570, 2230
AcuI	CTGAAG	5 cut(s)	320, 2316, 3255, 3512, 4197
AcvI	CACGTG	1 cut(s)	1408
AfiI	CCNNNNNNNGG	9 cut(s)	124, 614, 638, 947, 1433, 2018, 2019, 2333, 3317
AflIII	ACRYGT	4 cut(s)	674, 1600, 3039, 4087
AjnI	CCWGG	6 cut(s)	700, 1663, 2647, 3357, 3418, 3589
AleI	CACNNNNGTG	1 cut(s)	2116
AloI	GAACNNNNNNTCC	2 cut(s)	3105, 3137
Alw21I	GWGCWC	6 cut(s)	1680, 2857, 3184, 3574, 3954, 4208
Alw26I	GTCTC	6 cut(s)	385, 665, 1737, 2138, 2430, 3048
Alw44I	GTGCAC	1 cut(s)	2853
AlwI	GGATC	9 cut(s)	34, 623, 636, 848, 1237, 2797, 3146, 3159, 4409
Aor13HI	TCCGGA	1 cut(s)	3310
AoxI	GGCC	6 cut(s)	640, 703, 1465, 2998, 3102, 3219
ApaI	GGGCCC	1 cut(s)	644
ApaLI	GTGCAC	1 cut(s)	2853
ApoI	RAATTY	3 cut(s)	70, 570, 2230
ArsI	GACNNNNNNTTYG	2 cut(s)	2137, 2169
AseI	ATTAAT	1 cut(s)	1062
AspLEI	GCGC	2 cut(s)	1369, 1449
AsuC2I	CCSGG	2 cut(s)	44, 2494
AsuHPI	GGTGA	7 cut(s)	674, 1518, 1769, 1934, 2573, 3442, 4016
AvaII	GGWCC	6 cut(s)	19, 1303, 1649, 1704, 2710, 3070
BaeGI	GKGCMC	8 cut(s)	501, 644, 941, 1672, 2327, 2390, 2494, 2857
BaeI	ACNNNNGTAYC	2 cut(s)	3000, 3033
BalI	TGGCCA	1 cut(s)	705
BamHI	GGATCC	2 cut(s)	628, 3151
BanI	GGYRCC	2 cut(s)	1667, 4562
BanII	GRGCYC	4 cut(s)	644, 979, 3184, 3574
BarI	GAAGNNNNNNTAC	2 cut(s)	2619, 2651
BauI	CACGAG	1 cut(s)	3995
BbrPI	CACGTG	1 cut(s)	1408
BbsI	GAAGAC	3 cut(s)	311, 1466, 4181
Bbv12I	GWGCWC	6 cut(s)	1680, 2857, 3184, 3574, 3954, 4208
BceAI	ACGGC	1 cut(s)	1452
BciT130I	CCWGG	6 cut(s)	702, 1665, 2649, 3359, 3420, 3591
BciVI	GTATCC	2 cut(s)	3001, 4055
BclI	TGATCA	1 cut(s)	4471
BcnI	CCSGG	2 cut(s)	44, 2494
BcoDI	GTCTC	6 cut(s)	385, 665, 1737, 2138, 2430, 3048
BfaI	CTAG	9 cut(s)	906, 1673, 2544, 2895, 2934, 3065, 3483, 3503, 3788
BfoI	RGCGCY	1 cut(s)	1450
BfuAI	ACCTGC	3 cut(s)	2173, 3755, 3784
BfuI	GTATCC	2 cut(s)	3001, 4055
BlpI	GCTNAGC	1 cut(s)	1716
BmcAI	AGTACT	2 cut(s)	760, 3723
Bme1390I	CCNGG	8 cut(s)	44, 702, 1665, 2494, 2649, 3359, 3420, 3591
Bme18I	GGWCC	6 cut(s)	19, 1303, 1649, 1704, 2710, 3070
BmiI	GGNNCC	9 cut(s)	21, 630, 642, 1669, 2711, 2782, 3153, 3963, 4564
BmrFI	CCNGG	8 cut(s)	44, 702, 1665, 2494, 2649, 3359, 3420, 3591
BmrI	ACTGGG	3 cut(s)	532, 2767, 4047
BmuI	ACTGGG	3 cut(s)	532, 2767, 4047
BoxI	GACNNNNGTC	2 cut(s)	161, 291
BpiI	GAAGAC	3 cut(s)	311, 1466, 4181
BplI	GAGNNNNNCTC	2 cut(s)	3059, 3091
BpmI	CTGGAG	2 cut(s)	310, 3648
Bpu10I	CCTNAGC	2 cut(s)	261, 3216
Bpu1102I	GCTNAGC	1 cut(s)	1716
BpuEI	CTTGAG	3 cut(s)	963, 2028, 3746
BpuMI	CCSGG	2 cut(s)	44, 2494
BsaAI	YACGTR	1 cut(s)	1408
BsaBI	GATNNNNATC	4 cut(s)	597, 1832, 3738, 4473
BsaJI	CCNNGG	5 cut(s)	1050, 1663, 1862, 3590, 3929
BsaWI	WCCGGW	3 cut(s)	400, 3310, 4565
BsaXI	ACNNNNNCTCC	8 cut(s)	2740, 2770, 3105, 3135, 4295, 4325, 4575, 4605
Bsc4I	CCNNNNNNNGG	9 cut(s)	124, 614, 638, 947, 1433, 2018, 2019, 2333, 3317
Bse1I	ACTGG	8 cut(s)	293, 538, 1305, 1513, 2722, 2773, 3082, 4053
Bse3DI	GCAATG	3 cut(s)	662, 1182, 2325
Bse8I	GATNNNNATC	4 cut(s)	597, 1832, 3738, 4473
BseAI	TCCGGA	1 cut(s)	3310
BseBI	CCWGG	6 cut(s)	702, 1665, 2649, 3359, 3420, 3591
BseDI	CCNNGG	5 cut(s)	1050, 1663, 1862, 3590, 3929
BseJI	GATNNNNATC	4 cut(s)	597, 1832, 3738, 4473
BseLI	CCNNNNNNNGG	9 cut(s)	124, 614, 638, 947, 1433, 2018, 2019, 2333, 3317
BseMI	GCAATG	3 cut(s)	662, 1182, 2325
BseMII	CTCAG	6 cut(s)	1542, 1592, 1707, 1959, 2446, 3792
BseNI	ACTGG	8 cut(s)	293, 538, 1305, 1513, 2722, 2773, 3082, 4053
BseRI	GAGGAG	3 cut(s)	155, 1970, 4316
BseSI	GKGCMC	8 cut(s)	501, 644, 941, 1672, 2327, 2390, 2494, 2857
BseYI	CCCAGC	1 cut(s)	282
BsgI	GTGCAG	1 cut(s)	4277
Bsh1236I	CGCG	1 cut(s)	1392
BshFI	GGCC	6 cut(s)	642, 705, 1467, 3000, 3104, 3221
BshNI	GGYRCC	2 cut(s)	1667, 4562
BsiHKAI	GWGCWC	6 cut(s)	1680, 2857, 3184, 3574, 3954, 4208
BsiSI	CCGG	5 cut(s)	43, 401, 2494, 3311, 4566
BslI	CCNNNNNNNGG	9 cut(s)	124, 614, 638, 947, 1433, 2018, 2019, 2333, 3317
BsmAI	GTCTC	6 cut(s)	385, 665, 1737, 2138, 2430, 3048
BsmI	GAATGC	1 cut(s)	1122
BsnI	GGCC	6 cut(s)	642, 705, 1467, 3000, 3104, 3221
Bsp120I	GGGCCC	1 cut(s)	640
Bsp13I	TCCGGA	1 cut(s)	3310
Bsp1407I	TGTACA	2 cut(s)	1371, 2536
Bsp1720I	GCTNAGC	1 cut(s)	1716
BspANI	GGCC	6 cut(s)	642, 705, 1467, 3000, 3104, 3221
BspCNI	CTCAG	6 cut(s)	1543, 1591, 1708, 1960, 2445, 3793
BspEI	TCCGGA	1 cut(s)	3310
BspFNI	CGCG	1 cut(s)	1392
BspHI	TCATGA	2 cut(s)	1038, 1173
BspLI	GGNNCC	9 cut(s)	21, 630, 642, 1669, 2711, 2782, 3153, 3963, 4564
BspMAI	CTGCAG	4 cut(s)	33, 506, 2692, 2917
BspMI	ACCTGC	3 cut(s)	2173, 3755, 3784
BspPI	GGATC	9 cut(s)	34, 623, 636, 848, 1237, 2797, 3146, 3159, 4409
BspT107I	GGYRCC	2 cut(s)	1667, 4562
BsrDI	GCAATG	3 cut(s)	662, 1182, 2325
BsrGI	TGTACA	2 cut(s)	1371, 2536
BsrI	ACTGG	8 cut(s)	293, 538, 1305, 1513, 2722, 2773, 3082, 4053
BssECI	CCNNGG	5 cut(s)	1050, 1663, 1862, 3590, 3929
BssNAI	GTATAC	2 cut(s)	4314, 4547
BssSI	CACGAG	1 cut(s)	3995
BssT1I	CCWWGG	3 cut(s)	1050, 1862, 3929
Bst1107I	GTATAC	2 cut(s)	4314, 4547
Bst2BI	CACGAG	1 cut(s)	3995
Bst2UI	CCWGG	6 cut(s)	702, 1665, 2649, 3359, 3420, 3591
Bst6I	CTCTTC	5 cut(s)	383, 418, 2299, 4034, 4034
BstAUI	TGTACA	2 cut(s)	1371, 2536
BstBAI	YACGTR	1 cut(s)	1408
BstFNI	CGCG	1 cut(s)	1392
BstH2I	RGCGCY	1 cut(s)	1450
BstHHI	GCGC	2 cut(s)	1369, 1449
BstMAI	GTCTC	6 cut(s)	385, 665, 1737, 2138, 2430, 3048
BstNI	CCWGG	6 cut(s)	702, 1665, 2649, 3359, 3420, 3591
BstNSI	RCATGY	6 cut(s)	678, 1939, 1943, 2734, 3043, 3228
BstPAI	GACNNNNGTC	2 cut(s)	161, 291
BstSCI	CCNGG	8 cut(s)	42, 700, 1663, 2492, 2647, 3357, 3418, 3589
BstSLI	GKGCMC	8 cut(s)	501, 644, 941, 1672, 2327, 2390, 2494, 2857
BstUI	CGCG	1 cut(s)	1392
BstV2I	GAAGAC	3 cut(s)	311, 1466, 4181
BstX2I	RGATCY	4 cut(s)	628, 853, 2789, 3151
BstYI	RGATCY	4 cut(s)	628, 853, 2789, 3151
BstZ17I	GTATAC	2 cut(s)	4314, 4547
BsuI	GTATCC	2 cut(s)	3001, 4055
BsuRI	GGCC	6 cut(s)	642, 705, 1467, 3000, 3104, 3221
BtgZI	GCGATG	1 cut(s)	240
BtsI	GCAGTG	2 cut(s)	499, 1640
BveI	ACCTGC	3 cut(s)	2173, 3755, 3784
CciI	TCATGA	2 cut(s)	1038, 1173
CfoI	GCGC	2 cut(s)	1369, 1449
CseI	GACGC	1 cut(s)	4273
CsiI	ACCWGGT	1 cut(s)	2647
DraI	TTTAAA	2 cut(s)	1021, 3337
DrdI	GACNNNNNNGTC	3 cut(s)	55, 236, 3077
DseDI	GACNNNNNNGTC	3 cut(s)	55, 236, 3077
EaeI	YGGCCR	1 cut(s)	703
Eam1104I	CTCTTC	5 cut(s)	383, 418, 2299, 4034, 4034
EarI	CTCTTC	5 cut(s)	383, 418, 2299, 4034, 4034
Ecl136II	GAGCTC	2 cut(s)	3182, 3572
Eco130I	CCWWGG	3 cut(s)	1050, 1862, 3929
Eco147I	AGGCCT	1 cut(s)	3000
Eco24I	GRGCYC	4 cut(s)	644, 979, 3184, 3574
Eco47I	GGWCC	6 cut(s)	19, 1303, 1649, 1704, 2710, 3070
Eco53kI	GAGCTC	2 cut(s)	3182, 3572
Eco57I	CTGAAG	5 cut(s)	320, 2316, 3255, 3512, 4197
Eco72I	CACGTG	1 cut(s)	1408
EcoICRI	GAGCTC	2 cut(s)	3182, 3572
EcoO109I	RGGNCCY	3 cut(s)	19, 641, 3070
EcoRII	CCWGG	6 cut(s)	700, 1663, 2647, 3357, 3418, 3589
EcoT14I	CCWWGG	3 cut(s)	1050, 1862, 3929
EcoT22I	ATGCAT	1 cut(s)	1941
EcoT38I	GRGCYC	4 cut(s)	644, 979, 3184, 3574
ErhI	CCWWGG	3 cut(s)	1050, 1862, 3929
FauI	CCCGC	5 cut(s)	129, 331, 641, 3093, 3538
FauNDI	CATATG	3 cut(s)	1077, 3838, 4353
FbaI	TGATCA	1 cut(s)	4471
FblI	GTMKAC	3 cut(s)	1156, 4313, 4546
FriOI	GRGCYC	4 cut(s)	644, 979, 3184, 3574
FspBI	CTAG	9 cut(s)	906, 1673, 2544, 2895, 2934, 3065, 3483, 3503, 3788
GlaI	GCGC	2 cut(s)	1368, 1448
GsaI	CCCAGC	1 cut(s)	286
GsuI	CTGGAG	2 cut(s)	310, 3648
HaeII	RGCGCY	1 cut(s)	1450
HaeIII	GGCC	6 cut(s)	642, 705, 1467, 3000, 3104, 3221
HapII	CCGG	5 cut(s)	43, 401, 2494, 3311, 4566
HgaI	GACGC	1 cut(s)	4273
HhaI	GCGC	2 cut(s)	1369, 1449
Hin6I	GCGC	2 cut(s)	1367, 1447
HinP1I	GCGC	2 cut(s)	1367, 1447
HincII	GTYRAC	2 cut(s)	2831, 4507
HindII	GTYRAC	2 cut(s)	2831, 4507
HindIII	AAGCTT	2 cut(s)	1816, 3851
HpaII	CCGG	5 cut(s)	43, 401, 2494, 3311, 4566
HphI	GGTGA	7 cut(s)	674, 1518, 1769, 1934, 2573, 3442, 4016
Hpy99I	CGWCG	4 cut(s)	52, 167, 203, 2853
HpyCH4IV	ACGT	8 cut(s)	50, 198, 771, 1347, 1407, 1600, 4087, 4137
HpySE526I	ACGT	8 cut(s)	50, 198, 771, 1347, 1407, 1600, 4087, 4137
HspAI	GCGC	2 cut(s)	1367, 1447
Kpn2I	TCCGGA	1 cut(s)	3310
Ksp22I	TGATCA	1 cut(s)	4471
LmnI	GCTCC	4 cut(s)	3179, 3187, 3569, 3686
MabI	ACCWGGT	1 cut(s)	2647
MaeI	CTAG	9 cut(s)	906, 1673, 2544, 2895, 2934, 3065, 3483, 3503, 3788
MaeII	ACGT	8 cut(s)	50, 198, 771, 1347, 1407, 1600, 4087, 4137
MfeI	CAATTG	4 cut(s)	993, 2520, 3555, 4080
MflI	RGATCY	4 cut(s)	628, 853, 2789, 3151
MlsI	TGGCCA	1 cut(s)	705
MluNI	TGGCCA	1 cut(s)	705
MlyI	GAGTC	6 cut(s)	17, 301, 1039, 3301, 3666, 3832
MmeI	TCCRAC	8 cut(s)	220, 606, 727, 1093, 1537, 2307, 3404, 3749
Mox20I	TGGCCA	1 cut(s)	705
Mph1103I	ATGCAT	1 cut(s)	1941
MroI	TCCGGA	1 cut(s)	3310
MscI	TGGCCA	1 cut(s)	705
MslI	CAYNNNNRTG	6 cut(s)	1187, 1689, 1940, 2116, 4538, 4568
Msp20I	TGGCCA	1 cut(s)	705
MspA1I	CMGCKG	3 cut(s)	298, 3014, 3490
MspI	CCGG	5 cut(s)	43, 401, 2494, 3311, 4566
MspR9I	CCNGG	8 cut(s)	44, 702, 1665, 2494, 2649, 3359, 3420, 3591
MunI	CAATTG	4 cut(s)	993, 2520, 3555, 4080
Mva1269I	GAATGC	1 cut(s)	1122
MvaI	CCWGG	6 cut(s)	702, 1665, 2649, 3359, 3420, 3591
MvnI	CGCG	1 cut(s)	1392
NciI	CCSGG	2 cut(s)	44, 2494
NdeI	CATATG	3 cut(s)	1077, 3838, 4353
NlaIV	GGNNCC	9 cut(s)	21, 630, 642, 1669, 2711, 2782, 3153, 3963, 4564
NmuCI	GTSAC	8 cut(s)	662, 830, 874, 1757, 1922, 2546, 2600, 3475
NsiI	ATGCAT	1 cut(s)	1941
NspI	RCATGY	6 cut(s)	678, 1939, 1943, 2734, 3043, 3228
OliI	CACNNNNGTG	1 cut(s)	2116
PaeI	GCATGC	1 cut(s)	2734
PagI	TCATGA	2 cut(s)	1038, 1173
PaqCI	CACCTGC	1 cut(s)	2173
PceI	AGGCCT	1 cut(s)	3000
PciI	ACATGT	2 cut(s)	674, 3039
PcsI	WCGNNNNNNNCGW	3 cut(s)	56, 159, 168
PctI	GAATGC	1 cut(s)	1122
PfeI	GAWTC	9 cut(s)	114, 1046, 1560, 1886, 2470, 2514, 2675, 3409, 4410
PflMI	CCANNNNNTGG	2 cut(s)	1433, 2333
PfoI	TCCNGGA	1 cut(s)	3357
PleI	GAGTC	6 cut(s)	16, 300, 1038, 3301, 3665, 3831
PmaCI	CACGTG	1 cut(s)	1408
PmlI	CACGTG	1 cut(s)	1408
PpsI	GAGTC	6 cut(s)	16, 300, 1038, 3301, 3665, 3831
Ppu21I	YACGTR	1 cut(s)	1408
PpuMI	RGGWCCY	2 cut(s)	19, 3070
PscI	ACATGT	2 cut(s)	674, 3039
PshAI	GACNNNNGTC	2 cut(s)	161, 291
PshBI	ATTAAT	1 cut(s)	1062
PsiI	TTATAA	2 cut(s)	1905, 2072
Psp124BI	GAGCTC	2 cut(s)	3184, 3574
Psp5II	RGGWCCY	2 cut(s)	19, 3070
Psp6I	CCWGG	6 cut(s)	700, 1663, 2647, 3357, 3418, 3589
PspCI	CACGTG	1 cut(s)	1408
PspFI	CCCAGC	1 cut(s)	282
PspGI	CCWGG	6 cut(s)	700, 1663, 2647, 3357, 3418, 3589
PspN4I	GGNNCC	9 cut(s)	21, 630, 642, 1669, 2711, 2782, 3153, 3963, 4564
PspOMI	GGGCCC	1 cut(s)	640
PspPPI	RGGWCCY	2 cut(s)	19, 3070
PstI	CTGCAG	4 cut(s)	33, 506, 2692, 2917
PsuI	RGATCY	4 cut(s)	628, 853, 2789, 3151
PvuII	CAGCTG	2 cut(s)	3014, 3490
RseI	CAYNNNNRTG	6 cut(s)	1187, 1689, 1940, 2116, 4538, 4568
SacI	GAGCTC	2 cut(s)	3184, 3574
ScaI	AGTACT	2 cut(s)	760, 3723
SchI	GAGTC	6 cut(s)	17, 301, 1039, 3301, 3666, 3832
ScrFI	CCNGG	8 cut(s)	44, 702, 1665, 2494, 2649, 3359, 3420, 3591
SexAI	ACCWGGT	1 cut(s)	2647
SinI	GGWCC	6 cut(s)	19, 1303, 1649, 1704, 2710, 3070
SmiMI	CAYNNNNRTG	6 cut(s)	1187, 1689, 1940, 2116, 4538, 4568
SmlI	CTYRAG	3 cut(s)	978, 2043, 3725
SmoI	CTYRAG	3 cut(s)	978, 2043, 3725
SphI	GCATGC	1 cut(s)	2734
SseBI	AGGCCT	1 cut(s)	3000
SspI	AATATT	2 cut(s)	844, 1618
SspMI	CTAG	9 cut(s)	906, 1673, 2544, 2895, 2934, 3065, 3483, 3503, 3788
SstI	GAGCTC	2 cut(s)	3184, 3574
StuI	AGGCCT	1 cut(s)	3000
StyD4I	CCNGG	8 cut(s)	42, 700, 1663, 2492, 2647, 3357, 3418, 3589
StyI	CCWWGG	3 cut(s)	1050, 1862, 3929
TaiI	ACGT	8 cut(s)	53, 201, 774, 1350, 1410, 1603, 4090, 4140
TaqI	TCGA	6 cut(s)	165, 201, 583, 802, 1963, 2875
TatI	WGTACW	6 cut(s)	758, 902, 1371, 2536, 3721, 4161
TauI	GCSGC	3 cut(s)	225, 1948, 3107
TfiI	GAWTC	9 cut(s)	114, 1046, 1560, 1886, 2470, 2514, 2675, 3409, 4410
TseFI	GTSAC	8 cut(s)	662, 830, 874, 1757, 1922, 2546, 2600, 3475
Tsp45I	GTSAC	8 cut(s)	662, 830, 874, 1757, 1922, 2546, 2600, 3475
TspGWI	ACGGA	8 cut(s)	195, 1204, 2156, 2339, 2526, 3174, 3769, 4481
Van91I	CCANNNNNTGG	2 cut(s)	1433, 2333
VneI	GTGCAC	1 cut(s)	2853
VpaK11BI	GGWCC	6 cut(s)	19, 1303, 1649, 1704, 2710, 3070
VspI	ATTAAT	1 cut(s)	1062
XapI	RAATTY	3 cut(s)	70, 570, 2230
XbaI	TCTAGA	3 cut(s)	3064, 3482, 3502
XceI	RCATGY	6 cut(s)	678, 1939, 1943, 2734, 3043, 3228
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1781, 2187, 3467
XmiI	GTMKAC	3 cut(s)	1156, 4313, 4546
XspI	CTAG	9 cut(s)	906, 1673, 2544, 2895, 2934, 3065, 3483, 3503, 3788
ZrmI	AGTACT	2 cut(s)	760, 3723
Zsp2I	ATGCAT	1 cut(s)	1941

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.