Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003176

Physical Location & Seq

Genomic Coordinates Reverse (-)

12507238 .. 12511529

Gene Structure 4292 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1157000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4194 bp

ATGAAATCCGCCAACGTCAAAACCCTAGATTTCACCAGGCAGCCCTTTATCGAGCCCGATACGCACCGTACGGTAAAGGAACTGAAATTTACCGTTATGTCCCCGGCGGAGATTATGAAACTGGCGGAAGTTCAAATCTACATTGGCGCGTATTACAATTCCGGTTGGAAACCCATCGAAGGCGGCTTACTGGACCCGCGGCTCGGCCCGGCGAGCAAGCGGTTAATCTGCGCCACTTGTGGCGGGAACCACGTCGAGTGCCCGGGGCATTTTGGGTATTTGGCCCTCGCACTCCCCGTCTACAACGTCGGGTATCTGGGCACGGTTGTGAACGTCCTGAAGTGCATCTGCAAGAATTGTTCTGGGTTTCTACTGAAGGACGAAATGCGGGTGGAGTATTTGAGGAAGATGAGGAATCTGAAGGATCCTATGAAAAAAATCGAACTGATGAAAATTGTGGTGAAGAAATGTACTGCATTGTGTGCCGGTAATCGGCGGGCGGAGTGCTGGAAATGCGGGTATGTGAACGGGACGGTGAAGAAGGCGGTTGGGATGATCGGTATTCTTCACGACCGTTCGAAATTTGAAGATGTGATGGATGAGTTGAGAAAAACAATGGCGGAGACTAAAGTCTCCGCAGCGGGGTTGAGCGTGGAAGCTATGTTAGACCCCGCAAAAGTTTTCACTTTGTTGAAAGAGATGAGCGATGAGGATTGTGAGGTGCTTTATTTGTCTACTAGACCGGAGAATCTCTTGATAACCAACATTCTGGTGCCGCCTACTGTGATTCGCCCTTCGGTTTTGGTTGGGGGAGAAGTGAGCAACGAGAATGACATTACGGTGAGGTTGAAGCAGATCATTCAGTCGAATGCGCGCCTTACGCGCGATTTGTTTGATTTGAGGCTGGGGTATGCCCCCGGGGTTAAGCGGAGTTGGGATGAACTTCAGGTTGAGGTTGCGCAGTACATAAATAGTGAGGTTCGTGTGCCTTTCACAATGCAAGGTGGGAATCCGTTGAACGGTTTTGTGCAGCGGCTCAAGGGGAAGCAAGGGCGTTTTCGCGGGAATTTGTCTGCGAAACGTGTGGAATATACTGGAAGAAGTGTTATATCTCCTGACCCTAATCTGAAAATCAATGAGGTTGGAGTCCCCATTGAAATGGCTCAAATCTTAACGTACCCCGAACGGGTTACATGTCATAATATTGAGAAGTTGAGGAAGTGTGTGAGTAATGGACCTCATAAGTACCCCGGTGCCAGATCCTTACATCGTGATGGTGAAGAATTCTCCTTGGCGATTACGAGGATCAGTAAAAATCTGGCTGATGGACTCCAATACGGTGACATAGTTGGACGCCATCTACAAGACGGTGACATTCTTCTTTTCAACAGACAACCAAGCTTGCATAGGATGTCTTTCATGTCTCATCGGGCTAAGGTTATGCCTTGGAGGACGCTGAGGTTTAATGAATCTGTTTGCAATCCGTATAATGCTGATTTTGACGGCGATGAGATGAACATTCATGTCCCGCAAACAGAGGAGGCGCGGGCAGAGGCTGCTTTGTTGTTAGGGGTGGAAAACAATTTGTACACACCCAAAAATGGGGAGATCTTGGTTGCTTCAACTCAAGATTTTCTGACATCCTCTTTCCTTATAACGAGGAAGGACACATTTTATGACCGTTCATCATTTTCACTCATGTGTTCATATATGGGAAGTGGCATGGATCCAGTTGATCTTCCAACACCTGCCGTGATTAAACCAGTAGAGCTGTGGACAGGAAAGCAATTGTTTAGTGTTCTCGTACGCCCAAATGCAAATGTGAGAGTCTATCTGAATCTAACTGTCACGGAGAAGTCCTACACCAGCACCAAGACCGAAGATGGGAGAGAAATTGAAGCAATGTGCCCGAATGATGGGTTTGTCTATTTTCGTAACAGTGTGCTCATAGCCGGGCAACTTGGGAAGGCTACATTGGGAAATGGCAACAAGAATGGACTTTACTCTGTTCTTCTCAGAGACTATAAACCTCACGCAGCTGCTGCTTGCATGAATCGCTTAGCTAAGTTAAGCGCACAATGGATTGGTAACCACGGATTCTCAATTGGGATAAGCGATGTTCAGCCAAGTGGCAATTTGCGCGAAGAGAAAGAGAAGATAATTAAAGAAGGCTATGAAAAATGTTATGGCCAGATAAAGTTGTTTAATGAAGGGCAGCTCCAACTTATACCGGGTTCTGATGCAGCACAGTCGCTTGAGTCGGGGATAACTAGTGTATTGAACAACATCAGAGATCAAACCGGAAAGTTGTGTATGCAAAAGCTGCATTGGAGAAATAGTCCCTTGATCATGTCCCAGTGTGGTTCTAAAGGATCTGCCATCAATATCAGCCAGATGGTTGCTTGTGTTGGCCAGCAATCGGTTGGTGGTTGTCGTGCCCCCAACGGGTTCATAGACCGAAGCCTTCCACACTTCCCTAGAGGTGTAAAAACCCCTGCCGCTAAAGGGTTTGTTGCTAGCTCATTTTACAGCGGCTTGAGTCCTACAGAGTTTTTCTTCCATACAATGGGAGGGCGAGAGGGGCTTGTGGATACAGCTGTGAAAACAGCTGACACTGGGTACCTGAGTCGTAGATTGACCAAAGTTATGGAGGATCTGTGTGTTCAGTATGATAAAACCGTGAGAAATGCGAGTGGATGTATACTTCAGTTCTGTTATGGAGATGATGGGATGGATCCTGCAGTGATGGAAGGAAAAGATGGAGCTCCGTTGGACTTGCCCCGGTTGTTTCTGAAAGCGAAAGCCACATGTCCTGCCGGCAAAAATGAGTACGTGTCTCCTGATCAAGTGTCTGAAATAGTGAGAAGAAGGCTTTCTGAACCAGATATGACTCCTGAGGCCGGGTGCTCTGCCGGTTTCAAAACTTCTTTGGAAGAATTTTTGGATGAGTATGTAGAATCATTCAGAAAAAAACATGAGATATTTATGTTTGATGATGATCAAATCCCTGCTTGGAAGGAGAAGATTGTGCAAAATATCGCGGGTGTTACTTCTAGACAGTTGGAGGTTTTTCTTGATACTTGCATTTCACGTTACCATTCGAAGAAGATTGAAGGTGGAACAGCAATTGGAATAATCGGAGCTCAAAGTATTTGCGAGCCTGCGACGCAGATGACACTGAAAACTTTCCACTTTGCTGGAGTTGCAAGCATGAACATTACACTCGGAGTCCCCCGAATCAAGGAAATATTAGACGCGGTGAAGAACATCCACACGCCAATCATCACTGCGGCACTCGAATGTGATACTAACGAAAAATCTGCAAGGGTGGTAGCAGGGCGGATTGAGAGAACAAGTCTCGGGCAGATTGCTGAGAGTATACAGATTGTGATGACTGCAAGATCAGCATCTATAGTGATCACGCTCGATATGTCCATGATTCAAGATGCGCGCTTGTCCGTGGATGCCAATGTGGTGAAAGAATCGATTTTACAAATTCGGGGAATCAAACTAAAGTCAGAAAATATCAAGGTTTTGGATGTCAGAAAATTGGAAGTTTTTCCTGTAGAGACTGACAGGAACAAACTTTATTTTCGCCTTCACAGCTTGAAAAACATGCTCCCATCAATCACAGTAAGGGGCATAAACACAGTCAAGCGTGTCGTGATTAACAACGAAAAAGAAGCGAAAACAAATCACAAGAAGTACAAGCTGTTCGCCGAAGGCAGAGGGCTTCTAGAAGTGCTGGGGACTGAAGGAGTAGACGGATGCAGAACCACAAGCAATGACGTTCTGGAAGTGCAGAGGACGCTTGGGATCGAAGCGGCGCGAGTCTGCATCATTGAGGAGATAAAGAGCACGATGAAACAACATGGGATGAACATAGACTGCCGGCACATGATCTGCGTGGGGGATGTGATGACATACATGGGGGAAGTGCTTGGGCTGACGCGATTTGGAGCGAAGAAAATGGAGAAGAGTGTGATTGCATTGGCGTCGTTCGAGCAGGTGGCGGATCATCTGTTCAATGGCTGTGTGAATGGGAGAGTTGACGCGGTTAGAGGAGTGAGTGAGTGCATAATTATGGGTGTGCCAATTCGGGTTGGGACTGGAATGTTCAACATTAGAGAGGGAGGATTTTCTGGTTCGGGAGCTCATGATTCAAATGTCGAACTGCACTATGGGCCAGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002376 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0046483 GO:0048518 GO:0050789 GO:0050896 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1398

Amino Acids

154.7

Weight (kDa)

8.7

Isoelectric Point (pI)

40.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	23 - 357	4.3e-72	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	360 - 524	7e-70	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	528 - 707	7.7e-39	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	736 - 838	6.3e-29	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	845 - 1328	1.9e-86	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60040 AT5G60040
fragaria_vesca	FvH4_3g34290 FvH4_3g34290 FvH4_3g34290 FvH4_6g15631
malus_domestica	MD03G1106800.v1.1 MD09G1157000.v1.1
prunus_persica	Prupe.6G089600_v2.0.a1 Prupe.6G089600_v2.0.a1
pyrus_communis	pycom03g08620 pycom09g07570
rosa_chinensis	RchiOBHm_Chr3g0469301 RchiOBHm_Chr3g0469321 RchiOBHm_Chr5g0061861
rosa_laevigata	RLG00000024360 RLG00000035511
rosa_multiflora	Rmu_sc0004134.1_g000001 Rmu_sc0011641.1_g000013 Rmu_sc0011641.1_g000015 Rmu_sc0019271.1_g000001 Rmu_sc0022191.1_g000001
rosa_roxburghii	Rroxscaffold_1G00018870 Rroxscaffold_6G00411990 Rroxscaffold_6G00412000
rosa_rugosa	Rorug03G0100400 Rorug05G0344900 Rorug05G0345000
rosa_samantha	Rh3AG149800 Rh3BG173400 Rh3CG164500 Rh3DG202000 Rh5AG405500 Rh5BG418300 Rh5CG442800 Rh5DG432400
rosa_wichuraiana	Rw5G038070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1655
AarI	CACCTGC	2 cut(s)	1756, 3997
Acc16I	TGCGCA	1 cut(s)	960
Acc36I	ACCTGC	2 cut(s)	1756, 3997
Acc65I	GGTACC	1 cut(s)	2619
AccB1I	GGYRCC	3 cut(s)	772, 1253, 2619
AccB7I	CCANNNNNTGG	1 cut(s)	2566
AccI	GTMKAC	5 cut(s)	300, 734, 2701, 3379, 3762
AcoI	YGGCCR	2 cut(s)	2188, 2410
AcsI	RAATTY	6 cut(s)	86, 581, 1066, 1283, 2936, 3495
AcuI	CTGAAG	6 cut(s)	359, 395, 440, 929, 2690, 3774
AcyI	GRCGYC	2 cut(s)	1354, 3995
AflIII	ACRYGT	4 cut(s)	1081, 1193, 2807, 2832
AhlI	ACTAGT	1 cut(s)	2270
AjiI	CACGTC	1 cut(s)	253
AjnI	CCWGG	2 cut(s)	35, 4186
AjuI	GAANNNNNNNTTGG	2 cut(s)	1958, 1990
AleI	CACNNNNGTG	2 cut(s)	326, 1699
AloI	GAACNNNNNNTCC	2 cut(s)	4007, 4039
Alw21I	GWGCWC	6 cut(s)	1947, 2767, 2909, 3145, 3860, 4156
Alw26I	GTCTC	7 cut(s)	617, 637, 1428, 2013, 2841, 3362, 3563
AlwNI	CAGNNNCTG	2 cut(s)	1556, 2042
Ama87I	CYCGRG	3 cut(s)	262, 917, 3359
AoxI	GGCC	6 cut(s)	205, 282, 2188, 2410, 2898, 4184
ApoI	RAATTY	6 cut(s)	86, 581, 1066, 1283, 2936, 3495
Asp700I	GAANNNNTTC	3 cut(s)	2872, 3185, 3558
Asp718I	GGTACC	1 cut(s)	2619
AspS9I	GGNCC	5 cut(s)	193, 206, 283, 1235, 4184
AsuHPI	GGTGA	9 cut(s)	25, 472, 547, 853, 1289, 1352, 1382, 3271, 3487
AsuII	TTCGAA	2 cut(s)	578, 3101
AsuNHI	GCTAGC	1 cut(s)	2516
AvaI	CYCGRG	3 cut(s)	262, 917, 3359
AvaII	GGWCC	2 cut(s)	193, 1235
AxyI	CCTNAGG	1 cut(s)	2895
BaeGI	GKGCMC	4 cut(s)	263, 323, 1910, 2440
BaeI	ACNNNNGTAYC	4 cut(s)	2583, 2611, 2616, 2644
BalI	TGGCCA	2 cut(s)	2190, 2412
BamHI	GGATCC	3 cut(s)	424, 1726, 2734
BanI	GGYRCC	3 cut(s)	772, 1253, 2619
BanII	GRGCYC	4 cut(s)	57, 2767, 3145, 4156
BarI	GAAGNNNNNNTAC	2 cut(s)	171, 203
Bbv12I	GWGCWC	6 cut(s)	1947, 2767, 2909, 3145, 3860, 4156
BbvCI	CCTCAGC	1 cut(s)	1457
BceAI	ACGGC	2 cut(s)	1519, 1736
BcgI	CGANNNNNNTGC	6 cut(s)	2232, 2266, 3302, 3336, 3978, 4012
BciT130I	CCWGG	2 cut(s)	37, 4188
BciVI	GTATCC	1 cut(s)	2584
BclI	TGATCA	4 cut(s)	2346, 2842, 2998, 3417
BcoDI	GTCTC	7 cut(s)	617, 637, 1428, 2013, 2841, 3362, 3563
BcuI	ACTAGT	1 cut(s)	2270
BfaI	CTAG	8 cut(s)	26, 738, 2271, 2478, 2517, 3054, 3737, 4192
BfmI	CTRYAG	4 cut(s)	2544, 2739, 3411, 3564
BfuAI	ACCTGC	2 cut(s)	1756, 3997
BfuI	GTATCC	1 cut(s)	2584
BglII	AGATCT	1 cut(s)	1608
BlpI	GCTNAGC	1 cut(s)	2059
Bme18I	GGWCC	2 cut(s)	193, 1235
BmeT110I	CYCGRG	3 cut(s)	262, 917, 3359
BmgBI	CACGTC	1 cut(s)	253
BmgT120I	GGNCC	5 cut(s)	193, 206, 283, 1235, 4184
BmiI	GGNNCC	8 cut(s)	195, 248, 426, 774, 1255, 1728, 2621, 2736
BmrI	ACTGGG	2 cut(s)	2350, 2625
BmsI	GCATC	7 cut(s)	354, 2230, 3416, 3436, 3454, 3758, 3846
BmtI	GCTAGC	1 cut(s)	2520
BmuI	ACTGGG	2 cut(s)	2350, 2625
BoxI	GACNNNNGTC	1 cut(s)	629
BpmI	CTGGAG	1 cut(s)	3219
Bpu10I	CCTNAGC	2 cut(s)	1434, 1457
Bpu1102I	GCTNAGC	1 cut(s)	2059
Bpu14I	TTCGAA	2 cut(s)	578, 3101
BpuEI	CTTGAG	4 cut(s)	1022, 1611, 2276, 2557
Bsa29I	ATCGAT	1 cut(s)	3485
BsaAI	YACGTR	1 cut(s)	2833
BsaBI	GATNNNNATC	2 cut(s)	2997, 3406
BsaHI	GRCGYC	2 cut(s)	1354, 3995
BsaWI	WCCGGW	3 cut(s)	161, 742, 2300
BsaXI	ACNNNNNCTCC	4 cut(s)	2323, 2353, 2642, 2672
Bse118I	RCCGGY	4 cut(s)	485, 2816, 2912, 3891
Bse1I	ACTGG	8 cut(s)	126, 195, 1099, 1730, 1763, 2356, 2620, 4114
Bse21I	CCTNAGG	1 cut(s)	2895
Bse3DI	GCAATG	2 cut(s)	1908, 3790
Bse8I	GATNNNNATC	2 cut(s)	2997, 3406
BseBI	CCWGG	2 cut(s)	37, 4188
BseCI	ATCGAT	1 cut(s)	3485
BseJI	GATNNNNATC	2 cut(s)	2997, 3406
BseMI	GCAATG	2 cut(s)	1908, 3790
BseMII	CTCAG	5 cut(s)	1448, 2029, 2615, 2886, 3363
BseNI	ACTGG	8 cut(s)	126, 195, 1099, 1730, 1763, 2356, 2620, 4114
BsePI	GCGCGC	2 cut(s)	872, 3449
BseRI	GAGGAG	3 cut(s)	1553, 3860, 4077
BseSI	GKGCMC	4 cut(s)	263, 323, 1910, 2440
BseYI	CCCAGC	2 cut(s)	904, 3745
BsgI	GTGCAG	3 cut(s)	1049, 3821, 4160
Bsh1285I	CGRYCG	1 cut(s)	574
BshFI	GGCC	6 cut(s)	207, 284, 2190, 2412, 2900, 4186
BshNI	GGYRCC	3 cut(s)	772, 1253, 2619
BshVI	ATCGAT	1 cut(s)	3485
BsiEI	CGRYCG	1 cut(s)	574
BsiHKAI	GWGCWC	6 cut(s)	1947, 2767, 2909, 3145, 3860, 4156
BsiHKCI	CYCGRG	3 cut(s)	262, 917, 3359
BsiWI	CGTACG	2 cut(s)	68, 1804
BslFI	GGGAC	9 cut(s)	85, 544, 1133, 1511, 2325, 2338, 3215, 3763, 4120
BsmAI	GTCTC	7 cut(s)	617, 637, 1428, 2013, 2841, 3362, 3563
BsmFI	GGGAC	9 cut(s)	85, 544, 1133, 1511, 2325, 2338, 3215, 3763, 4120
BsmI	GAATGC	1 cut(s)	874
BsnI	GGCC	6 cut(s)	207, 284, 2190, 2412, 2900, 4186
BsoBI	CYCGRG	3 cut(s)	262, 917, 3359
Bsp119I	TTCGAA	2 cut(s)	578, 3101
Bsp1407I	TGTACA	1 cut(s)	1587
Bsp1720I	GCTNAGC	1 cut(s)	2059
BspANI	GGCC	6 cut(s)	207, 284, 2190, 2412, 2900, 4186
BspCNI	CTCAG	5 cut(s)	1449, 2028, 2616, 2887, 3364
BspDI	ATCGAT	1 cut(s)	3485
BspHI	TCATGA	1 cut(s)	4156
BspLI	GGNNCC	8 cut(s)	195, 248, 426, 774, 1255, 1728, 2621, 2736
BspMAI	CTGCAG	1 cut(s)	2743
BspMI	ACCTGC	2 cut(s)	1756, 3997
BspOI	GCTAGC	1 cut(s)	2520
BspT104I	TTCGAA	2 cut(s)	578, 3101
BspT107I	GGYRCC	3 cut(s)	772, 1253, 2619
BsrDI	GCAATG	2 cut(s)	1908, 3790
BsrFI	RCCGGY	4 cut(s)	485, 2816, 2912, 3891
BsrGI	TGTACA	1 cut(s)	1587
BsrI	ACTGG	8 cut(s)	126, 195, 1099, 1730, 1763, 2356, 2620, 4114
BssAI	RCCGGY	4 cut(s)	485, 2816, 2912, 3891
BssHII	GCGCGC	2 cut(s)	872, 3449
BssNAI	GTATAC	2 cut(s)	2702, 3380
BssNI	GRCGYC	2 cut(s)	1354, 3995
BssT1I	CCWWGG	2 cut(s)	1290, 1445
Bst1107I	GTATAC	2 cut(s)	2702, 3380
Bst2UI	CCWGG	2 cut(s)	37, 4188
Bst6I	CTCTTC	2 cut(s)	2139, 3971
BstACI	GRCGYC	2 cut(s)	1354, 3995
BstAPI	GCANNNNNTGC	4 cut(s)	482, 1556, 2042, 2323
BstAUI	TGTACA	1 cut(s)	1587
BstBAI	YACGTR	1 cut(s)	2833
BstBI	TTCGAA	2 cut(s)	578, 3101
BstDEI	CTNAG	8 cut(s)	1434, 1457, 2015, 2059, 2064, 2624, 2895, 3372
BstDSI	CCRYGG	3 cut(s)	197, 2092, 3459
BstEII	GGTNACC	1 cut(s)	2087
BstMAI	GTCTC	7 cut(s)	617, 637, 1428, 2013, 2841, 3362, 3563
BstMCI	CGRYCG	1 cut(s)	574
BstNI	CCWGG	2 cut(s)	37, 4188
BstNSI	RCATGY	3 cut(s)	1197, 2811, 3619
BstPAI	GACNNNNGTC	1 cut(s)	629
BstPI	GGTNACC	1 cut(s)	2087
BstSFI	CTRYAG	4 cut(s)	2544, 2739, 3411, 3564
BstSLI	GKGCMC	4 cut(s)	263, 323, 1910, 2440
BstX2I	RGATCY	7 cut(s)	424, 1259, 1608, 1726, 2372, 2653, 2734
BstXI	CCANNNNNNTGG	4 cut(s)	769, 1159, 2647, 3197
BstYI	RGATCY	7 cut(s)	424, 1259, 1608, 1726, 2372, 2653, 2734
BstZ17I	GTATAC	2 cut(s)	2702, 3380
Bsu15I	ATCGAT	1 cut(s)	3485
Bsu36I	CCTNAGG	1 cut(s)	2895
BsuI	GTATCC	1 cut(s)	2584
BsuRI	GGCC	6 cut(s)	207, 284, 2190, 2412, 2900, 4186
BsuTUI	ATCGAT	1 cut(s)	3485
BtgI	CCRYGG	3 cut(s)	197, 2092, 3459
BtgZI	GCGATG	3 cut(s)	720, 1521, 2130
BtrI	CACGTC	1 cut(s)	253
BtsI	GCAGTG	2 cut(s)	2748, 3285
BtsIMutI	CAGTG	6 cut(s)	1945, 2363, 2613, 2748, 3176, 3285
BveI	ACCTGC	2 cut(s)	1756, 3997
CaiI	CAGNNNCTG	2 cut(s)	1556, 2042
CciI	TCATGA	1 cut(s)	4156
Cfr10I	RCCGGY	4 cut(s)	485, 2816, 2912, 3891
Cfr13I	GGNCC	5 cut(s)	193, 206, 283, 1235, 4184
Cfr42I	CCGCGG	1 cut(s)	200
Cfr9I	CCCGGG	2 cut(s)	262, 917
ClaI	ATCGAT	1 cut(s)	3485
CseI	GACGC	8 cut(s)	1362, 1462, 3175, 3263, 3817, 3958, 3984, 4061
DdeI	CTNAG	8 cut(s)	1434, 1457, 2015, 2059, 2064, 2624, 2895, 3372
EaeI	YGGCCR	2 cut(s)	2188, 2410
Eam1104I	CTCTTC	2 cut(s)	2139, 3971
EarI	CTCTTC	2 cut(s)	2139, 3971
EciI	GGCGGA	6 cut(s)	122, 140, 515, 635, 3355, 4028
Ecl136II	GAGCTC	3 cut(s)	2765, 3143, 4154
Eco130I	CCWWGG	2 cut(s)	1290, 1445
Eco24I	GRGCYC	4 cut(s)	57, 2767, 3145, 4156
Eco47I	GGWCC	2 cut(s)	193, 1235
Eco53kI	GAGCTC	3 cut(s)	2765, 3143, 4154
Eco57I	CTGAAG	6 cut(s)	359, 395, 440, 929, 2690, 3774
Eco81I	CCTNAGG	1 cut(s)	2895
Eco88I	CYCGRG	3 cut(s)	262, 917, 3359
Eco91I	GGTNACC	1 cut(s)	2087
EcoICRI	GAGCTC	3 cut(s)	2765, 3143, 4154
EcoO65I	GGTNACC	1 cut(s)	2087
EcoRI	GAATTC	1 cut(s)	1283
EcoRII	CCWGG	2 cut(s)	35, 4186
EcoT14I	CCWWGG	2 cut(s)	1290, 1445
EcoT38I	GRGCYC	4 cut(s)	57, 2767, 3145, 4156
ErhI	CCWWGG	2 cut(s)	1290, 1445
FaqI	GGGAC	9 cut(s)	85, 544, 1133, 1511, 2325, 2338, 3215, 3763, 4120
FbaI	TGATCA	4 cut(s)	2346, 2842, 2998, 3417
FblI	GTMKAC	5 cut(s)	300, 734, 2701, 3379, 3762
FriOI	GRGCYC	4 cut(s)	57, 2767, 3145, 4156
FspBI	CTAG	8 cut(s)	26, 738, 2271, 2478, 2517, 3054, 3737, 4192
FspI	TGCGCA	1 cut(s)	960
GsaI	CCCAGC	2 cut(s)	908, 3749
GsuI	CTGGAG	1 cut(s)	3219
HaeIII	GGCC	6 cut(s)	207, 284, 2190, 2412, 2900, 4186
HgaI	GACGC	8 cut(s)	1362, 1462, 3175, 3263, 3817, 3958, 3984, 4061
Hin1I	GRCGYC	2 cut(s)	1354, 3995
HincII	GTYRAC	1 cut(s)	4051
HindII	GTYRAC	1 cut(s)	4051
HindIII	AAGCTT	1 cut(s)	1399
HphI	GGTGA	9 cut(s)	25, 472, 547, 853, 1289, 1352, 1382, 3271, 3487
Hpy99I	CGWCG	4 cut(s)	257, 311, 3169, 4000
HpyCH4IV	ACGT	9 cut(s)	15, 252, 306, 333, 1081, 1175, 2832, 3091, 3789
HpyF3I	CTNAG	8 cut(s)	1434, 1457, 2015, 2059, 2064, 2624, 2895, 3372
HpySE526I	ACGT	9 cut(s)	15, 252, 306, 333, 1081, 1175, 2832, 3091, 3789
Hsp92I	GRCGYC	2 cut(s)	1354, 3995
KpnI	GGTACC	1 cut(s)	2623
KroI	GCCGGC	2 cut(s)	2816, 3891
KroNI	GCCGGC	2 cut(s)	2818, 3893
Ksp22I	TGATCA	4 cut(s)	2346, 2842, 2998, 3417
KspI	CCGCGG	1 cut(s)	200
LmnI	GCTCC	7 cut(s)	2223, 2762, 2770, 3140, 3624, 3959, 4151
LweI	GCATC	7 cut(s)	354, 2230, 3416, 3436, 3454, 3758, 3846
MaeI	CTAG	8 cut(s)	26, 738, 2271, 2478, 2517, 3054, 3737, 4192
MaeII	ACGT	9 cut(s)	15, 252, 306, 333, 1081, 1175, 2832, 3091, 3789
MaeIII	GTNAC	8 cut(s)	1189, 1340, 1370, 1846, 1934, 2087, 3046, 3092
MfeI	CAATTG	3 cut(s)	1787, 2103, 3126
MflI	RGATCY	7 cut(s)	424, 1259, 1608, 1726, 2372, 2653, 2734
MlsI	TGGCCA	2 cut(s)	2190, 2412
MluNI	TGGCCA	2 cut(s)	2190, 2412
MlyI	GAGTC	9 cut(s)	1155, 1323, 1836, 2267, 2548, 2635, 2884, 3237, 3840
MmeI	TCCRAC	7 cut(s)	146, 1123, 1330, 1766, 2245, 2751, 3042
Mox20I	TGGCCA	2 cut(s)	2190, 2412
MroNI	GCCGGC	2 cut(s)	2816, 3891
MroXI	GAANNNNTTC	3 cut(s)	2872, 3185, 3558
MscI	TGGCCA	2 cut(s)	2190, 2412
MseI	TTAA	9 cut(s)	224, 924, 1172, 1464, 1758, 2069, 2163, 2205, 3669
MslI	CAYNNNNRTG	8 cut(s)	326, 770, 839, 1157, 1272, 1699, 3298, 4082
Msp20I	TGGCCA	2 cut(s)	2190, 2412
MspA1I	CMGCKG	7 cut(s)	199, 641, 1033, 2039, 2532, 2597, 2609
MunI	CAATTG	3 cut(s)	1787, 2103, 3126
Mva1269I	GAATGC	1 cut(s)	874
MvaI	CCWGG	2 cut(s)	37, 4188
NaeI	GCCGGC	2 cut(s)	2818, 3893
NgoMIV	GCCGGC	2 cut(s)	2816, 3891
NheI	GCTAGC	1 cut(s)	2516
NlaIV	GGNNCC	8 cut(s)	195, 248, 426, 774, 1255, 1728, 2621, 2736
NmeAIII	GCCGAG	1 cut(s)	183
NmuCI	GTSAC	3 cut(s)	1340, 1370, 1846
NsbI	TGCGCA	1 cut(s)	960
NspI	RCATGY	3 cut(s)	1197, 2811, 3619
NspV	TTCGAA	2 cut(s)	578, 3101
OliI	CACNNNNGTG	2 cut(s)	326, 1699
PagI	TCATGA	1 cut(s)	4156
PaqCI	CACCTGC	2 cut(s)	1756, 3997
PauI	GCGCGC	2 cut(s)	872, 3449
PciI	ACATGT	2 cut(s)	1193, 2807
PcsI	WCGNNNNNNNCGW	1 cut(s)	294
PctI	GAATGC	1 cut(s)	874
PdiI	GCCGGC	2 cut(s)	2818, 3893
PdmI	GAANNNNTTC	3 cut(s)	2872, 3185, 3558
Pfl23II	CGTACG	2 cut(s)	68, 1804
PflMI	CCANNNNNTGG	1 cut(s)	2566
PleI	GAGTC	9 cut(s)	1154, 1323, 1835, 2266, 2547, 2634, 2884, 3236, 3839
PpsI	GAGTC	9 cut(s)	1154, 1323, 1835, 2266, 2547, 2634, 2884, 3236, 3839
Ppu21I	YACGTR	1 cut(s)	2833
PscI	ACATGT	2 cut(s)	1193, 2807
PshAI	GACNNNNGTC	1 cut(s)	629
PsiI	TTATAA	1 cut(s)	1655
Psp124BI	GAGCTC	3 cut(s)	2767, 3145, 4156
Psp6I	CCWGG	2 cut(s)	35, 4186
PspEI	GGTNACC	1 cut(s)	2087
PspFI	CCCAGC	2 cut(s)	904, 3745
PspGI	CCWGG	2 cut(s)	35, 4186
PspLI	CGTACG	2 cut(s)	68, 1804
PspN4I	GGNNCC	8 cut(s)	195, 248, 426, 774, 1255, 1728, 2621, 2736
PspPI	GGNCC	5 cut(s)	193, 206, 283, 1235, 4184
PsrI	GAACNNNNNNTAC	2 cut(s)	3698, 3730
PstI	CTGCAG	1 cut(s)	2743
PstNI	CAGNNNCTG	2 cut(s)	1556, 2042
PsuI	RGATCY	7 cut(s)	424, 1259, 1608, 1726, 2372, 2653, 2734
PteI	GCGCGC	2 cut(s)	872, 3449
PvuII	CAGCTG	3 cut(s)	2039, 2597, 2609
RseI	CAYNNNNRTG	8 cut(s)	326, 770, 839, 1157, 1272, 1699, 3298, 4082
SacI	GAGCTC	3 cut(s)	2767, 3145, 4156
SacII	CCGCGG	1 cut(s)	200
SaqAI	TTAA	9 cut(s)	224, 924, 1172, 1464, 1758, 2069, 2163, 2205, 3669
Sau96I	GGNCC	5 cut(s)	193, 206, 283, 1235, 4184
SchI	GAGTC	9 cut(s)	1155, 1323, 1836, 2267, 2548, 2635, 2884, 3237, 3840
SfaNI	GCATC	7 cut(s)	354, 2230, 3416, 3436, 3454, 3758, 3846
SfcI	CTRYAG	4 cut(s)	2544, 2739, 3411, 3564
Sfr303I	CCGCGG	1 cut(s)	200
SfuI	TTCGAA	2 cut(s)	578, 3101
SgrBI	CCGCGG	1 cut(s)	200
SinI	GGWCC	2 cut(s)	193, 1235
SmaI	CCCGGG	2 cut(s)	264, 919
SmiMI	CAYNNNNRTG	8 cut(s)	326, 770, 839, 1157, 1272, 1699, 3298, 4082
SmlI	CTYRAG	4 cut(s)	1037, 1626, 2255, 2536
SmoI	CTYRAG	4 cut(s)	1037, 1626, 2255, 2536
SpeI	ACTAGT	1 cut(s)	2270
SspI	AATATT	2 cut(s)	1204, 3249
SspMI	CTAG	8 cut(s)	26, 738, 2271, 2478, 2517, 3054, 3737, 4192
SstI	GAGCTC	3 cut(s)	2767, 3145, 4156
StyI	CCWWGG	2 cut(s)	1290, 1445
TaiI	ACGT	9 cut(s)	18, 255, 309, 336, 1084, 1178, 2835, 3094, 3792
TaqII	GACCGA	2 cut(s)	1892, 2472
TatI	WGTACW	4 cut(s)	470, 963, 1587, 3705
TauI	GCSGC	8 cut(s)	186, 202, 778, 1036, 2501, 2535, 3293, 3827
Tru1I	TTAA	9 cut(s)	224, 924, 1172, 1464, 1758, 2069, 2163, 2205, 3669
Tru9I	TTAA	9 cut(s)	224, 924, 1172, 1464, 1758, 2069, 2163, 2205, 3669
TscAI	CASTG	6 cut(s)	1945, 2363, 2620, 2748, 3183, 3292
TseFI	GTSAC	3 cut(s)	1340, 1370, 1846
Tsp45I	GTSAC	3 cut(s)	1340, 1370, 1846
TspGWI	ACGGA	7 cut(s)	1002, 1473, 1865, 2109, 2757, 3448, 3780
TspMI	CCCGGG	2 cut(s)	262, 917
TspRI	CASTG	6 cut(s)	1945, 2363, 2620, 2748, 3183, 3292
Van91I	CCANNNNNTGG	1 cut(s)	2566
VpaK11BI	GGWCC	2 cut(s)	193, 1235
XapI	RAATTY	6 cut(s)	86, 581, 1066, 1283, 2936, 3495
XbaI	TCTAGA	2 cut(s)	3053, 3736
XceI	RCATGY	3 cut(s)	1197, 2811, 3619
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1770, 2420
XmaI	CCCGGG	2 cut(s)	262, 917
XmiI	GTMKAC	5 cut(s)	300, 734, 2701, 3379, 3762
XmnI	GAANNNNTTC	3 cut(s)	2872, 3185, 3558
XspI	CTAG	8 cut(s)	26, 738, 2271, 2478, 2517, 3054, 3737, 4192

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.