Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0003176

Physical Location & Seq

Genomic Coordinates Reverse (-)

67280973 .. 67307073

Gene Structure 26101 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G038070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4443 bp

ATGATAGTGGTGAAGTCGTACATGAACAAATTGCAGAGTCCGCTGAAGAAGACACAAGGGTACACTCCCGCCCTTTACACCAAACGCAGCCCCTTCAACCTTCAACCCTCCCTCTTTGCTTTCTGGCTGGAGAAGAGACTTCTACCGGATTTTCTCTCTTCAATTTTGATAGAAAAGATGATGAATAAAGTCCAAGCCAAAACATTAGCCTTCACAAAAGAGCCTTTCATTGAAGAAGAAGGGCACTGCAGAATAGCACAATGGCAATTCAATGCTCTGTCCCAGTCAGAGATCAGCAAAATGGCTGCAACTCAAATTTTTCTGGGTCGATATTACGATAGCAATCAAAAACCAATCAAAGGAGGCTTGTTGGATCCCCGCTTGGGCCCTGCAAATAGGCAATGCGGTGACTGTGAGACATGTGGTGCGAAACTTTCAGATTGTCCTGGCCACTTTGGATATTTGGTGCTTGCTGTCCCTGTATTCAATGTTGGATATTTAAGTACTGTTGTGGACGTTTTGAAGTGCATTTGTAAGTCATGCTCTCGAATAATTTTGGATGAAAAATCACGCAGTCACTTTTTGAATATTGTGAGAGATCCCAAAAAGGATAAAAGCGTGACAATGAAATCAATAGTGAAGAAGTGTACTAGCATGGCAAGCAATAATAGATCAGTGAAGTGCCCGAAATGTGGATATATGAATGGTTCAGTGAAAAGGGCTCAAGGTATGCTGGCAATTGTTCATGGTGCTGTGGATGACTTTAAATCAGCGAGTCCTGTCATGAAGGATTCCAAGGCATCCATTAATCTCCAACCTCATATGCTTAACCCTGTTAAAGTTTTTTCTTTGTTCAAAAGAATGCTTGATGAGGATTGTGAACTTCTTAATCTTAAAAGTAGACCAGAGAACCTCATCATGACAACCATTGCTGTGCCACCTATAGCCATTCGTCCTTCCGTTCTTGTGGATGGATCACAGAGTAACGAAAATGACATTACAGAGAGGTTGAAGAAAATTATTCAAACAAACGCTAATGTTCAACTGGACCTATCAGAAGGTTCTACCACAAGATTTTTGTCAAATTGGGACGTACTTCAAGCCGAAGTTGCGCTGTACATAAACAGTGATGTTCGCGGTATTCCTTATCACGTGCAACTAAATACCAAGCCATTGAATGGTTTGGTACAGCGCCTCAAAGGGAAGACGGGCCGTTTTCGTGGAAACCTATGTGGGAAAAGGGTTGAATATACTGGCAGAACTGTTATTTCACCCGACCCTAATCTGAAAATTACTGAGGTCGGAATCCCTATCCAAATGGCTCAGATTTTAAGTTATCCAGAACGTGTGTCCCATCATAATATTGAAAAGTTGAAGCAGTGTGTATTTAATGGACCTTCTAAGTACCCTGGTGCCCTAGTGCTCAAACATCAAAATGGCGAGGAATGGTCCTTGAAGGCTGAGCGACAAAGGAAGTCTCATGCTGATAATCTCCAATATGGTGACATAGTTGATCGCCACTTAGAAGATGGGGACATTGTTCTTTTCAACAGACAACCAAGCTTGCATCGGATGTCCATCATGTGTCATAGGGCAAGGATTATGCCTTGGAGAACATTGAGGTTTAATGAATCTGTATGCAACCCTTATAATGCTGATTTTGATGGTGACGAAATGAACATGCATGTGCCGCAAACAGAGGAGGCTCGAACTGAGGCTGTTATGTTGATGGGGGTGCAAAACAATCTATGCACTCCCAAAAATGGGGAGATTTTGGTTGCTTCCACTCAAGATTTTCTAACATCTTCTTTCCTTATAACAAGGAAGGACACATTTTATGACCGTGCATCTTTTTCACTCATGTGTTCGTACATGGGGGATGGCACGGAGACTGTGGATTTGCCAACACCTGCTGTAATTAAGCCAATAGAGCTATGGACAGGAAAGCAGCTATATAGTGTGTTAGTTCGCCCAAATTCAAATGTGAGAGTATATCTAAACCTAACTGTTAGAGAGAAATCCTACTCCAACACAAAAACTGAAGATGGAAGAGAAACAGAAGCAATGTGCCCAAACGATGGGTTTGTCTATTTCCGTAACAGTGAGCTATTATCTGGGCAACTTGGGAAGGGCACTTTAGGAAATGGCAATAAGGATGGACTTTTCTCTGTTCTTCTCAGAGACTATAAAGCTCATGCTGCTGCTTCTTGCATGAATCGTGTAGCTAAATTGAGTGCCCGGTGGATTGGTAATCACGGATTCTCAATTGGGATTAGTGATGTACAACCTAGTGACATCTTGTACGGTGAGAAAGAAAAAATCATTAAGAAAGGTTATGACAAATGTGACGACCAGATAAAGTTGTATAAAGAAGGACAGCTACGACTTGCACCTGGTTGTGATGCTGCCCAATCACTTGAATCTGGGATTACTGCAGTATTAAACGATATTAGGGACCAAACTGGAAAGCTGTGCATGCAAACGCTACATTGGAGAAACAGTCCCCTGATTATGTCCCAGTGTGGTTCCAAAGGATCTGCTATCAATATCAGCCAGATGGTTGCTTGTGTTGGTCAACAATCAGTTGGTGGTCGTCGTGCACCAAATGGTTTCATAGATCGAAGCCTTCCTCACTTTCCTAGAAAAGAAAATACCCCTGCAGCTAAAGGCTTTGTTGCTAGTTCCTTTTACAGTGGCTTGACAGCTACAGAGTTCTTTTTTCATACAATGGGAGGGCGAGAAGGCCTTGTGGATACAGCTGTAAAAACTGCTGACACAGGGTACATGTCTCGTAGATTGTCTAAAGTTCTAGAGGACCTCTCTGTCCAGTATGATAAAACAGTGCGGGCCGCAAATGGAGGTATTGTTCAGTTTTGTTATGGGGATGACGGTATGGATCCTGCCATGATGGAAGGAAAAAGTGGAGCTCCGTTAGACTTCTACCGATTGTTTTTCAAAGCTAAGGCCACATGCCCTGCTGAAGAAAGTAAAAGCCTTTCTCCTGATGAAGTGTCTGAAATAGTGAGAAGCAGGCTTTCGGAACAAGATATGACTCCGGATGGTGGTTGCTCTATGGGTTTTAAAAGTTCTATAGAGCAATTCCTGGATGAATATGTAAAAGCATTAAGAAAAACACAGGAGGCTTTTTTATTGAATCAAAGTCCTGGTTGGAAGGTGAAGTCTGCAACTGTAGAGAAGATTGTCCAAAATATATCTGGTGTCACTTCTAGACAGCTGAAGGTTTTTCTAGATACTTGTATTTCTCGTTATCATTCAAAAAAAGTTGATGCGGGAACAGCAATTGGAGTAATTGGAGCTCAAAGCATTGGCGAGCCTGGGACACAGATGACATTAAAAACTTTTCACTTTGCTGGAGTTGCAAGCATGAATGTTACACTTGGAGTCCCTCGTATCAAGGAAATAATAAATGGAGCAAAAAATATCCATACCCCTATCATCACTACAGTACTTGAGTGTGATAATAATCCGATGATTGCACGGATGGTAGCAGGTCGGATTGAAAAAACAAATCTAGGGCAGGTTGCTGAGTGTATAGAGATTGTAATGAGTCCAAGAGCAGCATATGTAGCCATCAAGCTTGATATGTTTGTGATACAAGATGCACACTTGTCTATAGATGCCAATGTTGTAAAACAATCAATTTTGGGAACTCCAAGGATTAAACTGAAACATGAGCACATTAGGGTTCCAGATAATCAAACAGTTGAAGTTCATTCTCACGAGAAGGATAGAAGAAAACTCCATTTTCACCTCTTCTATTTGAAGAGTATCCTCCCCAGTGTTATAGTAAGGGGTATAAAGACAATTGAACGTGTTGTCATTGATGAAGAAAAAGTTAAAAGGGACAAAAAAGATATTGACGTTAAATGTGGAGAAGAAAAGAAGTACAAGTTATTTGCTGAAGGCAAGGGTCTTCTGGCAGTAATGAGCACTAAAGGAGTAGATGGTTGTAAAACCACAAGTAATGATATTATTGAAGTGCAGCAGACGCTTGGAATTGAAGCTGCAAGAACTTGTATCATTGAGGAGATAAAGTATACTATGAAATCACACGGGATGAACATAGATGATCGTCATATGATGCTTTTAGCGGATGTAATGACATTCAGAGGTTTAGTTCTTGGGATCACAAGATTCGGCGTTCAAAAGATGGGCAAAAGTGTATTGATGCTGGCTTCATTTGAGCGAACGGATGATCATCTATTTAATGCTTGCGTTCAGGGGACAGTTGACAAAATTGAAGGAGTAAGTGAAAGCATAATCATGGGTATACCAATGCCAATAGGCACCGGAATGTTCAAAATTAAACAACGAGAAAAACACAGCAGCAATCTGCAACAAAACCAGTCCTATGGACTGGATGAAATCTCAGATAGACCCATGTGGTTGCCTCCCCTTAATTTGCGTCATGAAGACGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002376 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0046483 GO:0048518 GO:0050789 GO:0050896 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1480

Amino Acids

165.07

Weight (kDa)

8.98

Isoelectric Point (pI)

41.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	84 - 410	6e-76	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	412 - 579	3.2e-74	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	582 - 761	7.2e-38	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	789 - 892	4.3e-29	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	899 - 1394	1.5e-88	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60040 AT5G60040
fragaria_vesca	FvH4_3g34290 FvH4_3g34290 FvH4_3g34290 FvH4_6g15631
malus_domestica	MD03G1106800.v1.1 MD09G1157000.v1.1
prunus_persica	Prupe.6G089600_v2.0.a1 Prupe.6G089600_v2.0.a1
pyrus_communis	pycom03g08620 pycom09g07570
rosa_chinensis	RchiOBHm_Chr3g0469301 RchiOBHm_Chr3g0469321 RchiOBHm_Chr5g0061861
rosa_laevigata	RLG00000024360 RLG00000035511
rosa_multiflora	Rmu_sc0004134.1_g000001 Rmu_sc0011641.1_g000013 Rmu_sc0011641.1_g000015 Rmu_sc0019271.1_g000001 Rmu_sc0022191.1_g000001
rosa_roxburghii	Rroxscaffold_1G00018870 Rroxscaffold_6G00411990 Rroxscaffold_6G00412000
rosa_rugosa	Rorug03G0100400 Rorug05G0344900 Rorug05G0345000
rosa_samantha	Rh3AG149800 Rh3BG173400 Rh3CG164500 Rh3DG202000 Rh5AG405500 Rh5BG418300 Rh5CG442800 Rh5DG432400
rosa_wichuraiana	Rw5G038070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1650, 1817
AarI	CACCTGC	1 cut(s)	1918
AasI	GACNNNNNNGTC	1 cut(s)	2822
AatII	GACGTC	1 cut(s)	4440
Acc36I	ACCTGC	3 cut(s)	1918, 3500, 3529
AccB1I	GGYRCC	2 cut(s)	1412, 4307
AccB7I	CCANNNNNTGG	2 cut(s)	1178, 2078
AccI	GTMKAC	3 cut(s)	901, 4058, 4291
AccII	CGCG	1 cut(s)	1137
AccIII	TCCGGA	1 cut(s)	3055
AclWI	GGATC	8 cut(s)	368, 381, 593, 982, 2542, 2891, 2904, 4154
AcoI	YGGCCR	1 cut(s)	448
AcsI	RAATTY	2 cut(s)	315, 1975
AcuI	CTGAAG	5 cut(s)	65, 2061, 3000, 3257, 3942
AcvI	CACGTG	1 cut(s)	1153
AcyI	GRCGYC	1 cut(s)	4437
AfiI	CCNNNNNNNGG	8 cut(s)	359, 383, 692, 1178, 1763, 1764, 2078, 3062
AflIII	ACRYGT	4 cut(s)	419, 1345, 2784, 3832
AjnI	CCWGG	6 cut(s)	445, 1408, 2392, 3102, 3163, 3334
AleI	CACNNNNGTG	1 cut(s)	1861
AloI	GAACNNNNNNTCC	2 cut(s)	2850, 2882
Alw21I	GWGCWC	6 cut(s)	1425, 2602, 2929, 3319, 3699, 3953
Alw26I	GTCTC	6 cut(s)	130, 410, 1482, 1883, 2175, 2793
Alw44I	GTGCAC	1 cut(s)	2598
AlwI	GGATC	8 cut(s)	368, 381, 593, 982, 2542, 2891, 2904, 4154
Aor13HI	TCCGGA	1 cut(s)	3055
AoxI	GGCC	6 cut(s)	385, 448, 1210, 2743, 2847, 2964
ApaI	GGGCCC	1 cut(s)	389
ApaLI	GTGCAC	1 cut(s)	2598
ApoI	RAATTY	2 cut(s)	315, 1975
ArsI	GACNNNNNNTTYG	2 cut(s)	1882, 1914
AseI	ATTAAT	1 cut(s)	807
AspLEI	GCGC	2 cut(s)	1114, 1194
AspS9I	GGNCC	9 cut(s)	385, 386, 1048, 1210, 1394, 1449, 2455, 2815, 2847
AsuC2I	CCSGG	1 cut(s)	2239
AsuHPI	GGTGA	8 cut(s)	22, 419, 1263, 1514, 1679, 2318, 3187, 3761
AvaII	GGWCC	5 cut(s)	1048, 1394, 1449, 2455, 2815
BaeGI	GKGCMC	8 cut(s)	246, 389, 686, 1417, 2072, 2135, 2239, 2602
BaeI	ACNNNNGTAYC	2 cut(s)	2745, 2778
BalI	TGGCCA	1 cut(s)	450
BamHI	GGATCC	2 cut(s)	373, 2896
BanI	GGYRCC	2 cut(s)	1412, 4307
BanII	GRGCYC	4 cut(s)	389, 724, 2929, 3319
BarI	GAAGNNNNNNTAC	2 cut(s)	2364, 2396
BauI	CACGAG	1 cut(s)	3740
BbrPI	CACGTG	1 cut(s)	1153
BbsI	GAAGAC	3 cut(s)	56, 1211, 3926
Bbv12I	GWGCWC	6 cut(s)	1425, 2602, 2929, 3319, 3699, 3953
BceAI	ACGGC	1 cut(s)	1197
BciT130I	CCWGG	6 cut(s)	447, 1410, 2394, 3104, 3165, 3336
BciVI	GTATCC	2 cut(s)	2746, 3800
BclI	TGATCA	1 cut(s)	4216
BcnI	CCSGG	1 cut(s)	2239
BcoDI	GTCTC	6 cut(s)	130, 410, 1482, 1883, 2175, 2793
BfmI	CTRYAG	9 cut(s)	247, 942, 2433, 2658, 2706, 3090, 3189, 3462, 3633
BfoI	RGCGCY	1 cut(s)	1195
BfuAI	ACCTGC	3 cut(s)	1918, 3500, 3529
BfuI	GTATCC	2 cut(s)	2746, 3800
BlpI	GCTNAGC	1 cut(s)	1461
BmcAI	AGTACT	2 cut(s)	505, 3468
Bme1390I	CCNGG	7 cut(s)	447, 1410, 2239, 2394, 3104, 3165, 3336
Bme18I	GGWCC	5 cut(s)	1048, 1394, 1449, 2455, 2815
BmgT120I	GGNCC	9 cut(s)	385, 386, 1048, 1210, 1394, 1449, 2455, 2815, 2847
BmiI	GGNNCC	8 cut(s)	375, 387, 1414, 2456, 2527, 2898, 3708, 4309
BmrFI	CCNGG	7 cut(s)	447, 1410, 2239, 2394, 3104, 3165, 3336
BmrI	ACTGGG	3 cut(s)	277, 2512, 3792
BmsI	GCATC	9 cut(s)	809, 1576, 1856, 2392, 3277, 3610, 3628, 4092, 4179
BmuI	ACTGGG	3 cut(s)	277, 2512, 3792
BpiI	GAAGAC	3 cut(s)	56, 1211, 3926
BplI	GAGNNNNNCTC	2 cut(s)	2804, 2836
BpmI	CTGGAG	2 cut(s)	149, 3393
Bpu10I	CCTNAGC	1 cut(s)	2961
Bpu1102I	GCTNAGC	1 cut(s)	1461
BpuEI	CTTGAG	3 cut(s)	708, 1773, 3491
BpuMI	CCSGG	1 cut(s)	2239
BsaAI	YACGTR	1 cut(s)	1153
BsaBI	GATNNNNATC	4 cut(s)	342, 1577, 3483, 4218
BsaHI	GRCGYC	1 cut(s)	4437
BsaJI	CCNNGG	5 cut(s)	795, 1408, 1607, 3335, 3674
BsaWI	WCCGGW	3 cut(s)	145, 3055, 4310
BsaXI	ACNNNNNCTCC	6 cut(s)	2485, 2515, 2850, 2880, 4040, 4070
Bsc4I	CCNNNNNNNGG	8 cut(s)	359, 383, 692, 1178, 1763, 1764, 2078, 3062
Bse1I	ACTGG	9 cut(s)	283, 1050, 1258, 2467, 2518, 2827, 3798, 4366, 4383
Bse3DI	GCAATG	3 cut(s)	407, 927, 2070
Bse8I	GATNNNNATC	4 cut(s)	342, 1577, 3483, 4218
BseAI	TCCGGA	1 cut(s)	3055
BseBI	CCWGG	6 cut(s)	447, 1410, 2394, 3104, 3165, 3336
BseDI	CCNNGG	5 cut(s)	795, 1408, 1607, 3335, 3674
BseJI	GATNNNNATC	4 cut(s)	342, 1577, 3483, 4218
BseLI	CCNNNNNNNGG	8 cut(s)	359, 383, 692, 1178, 1763, 1764, 2078, 3062
BseMI	GCAATG	3 cut(s)	407, 927, 2070
BseMII	CTCAG	7 cut(s)	1287, 1337, 1452, 1704, 2191, 3537, 4404
BseNI	ACTGG	9 cut(s)	283, 1050, 1258, 2467, 2518, 2827, 3798, 4366, 4383
BseRI	GAGGAG	2 cut(s)	1715, 4061
BseSI	GKGCMC	8 cut(s)	246, 389, 686, 1417, 2072, 2135, 2239, 2602
BsgI	GTGCAG	1 cut(s)	4022
Bsh1236I	CGCG	1 cut(s)	1137
BshFI	GGCC	6 cut(s)	387, 450, 1212, 2745, 2849, 2966
BshNI	GGYRCC	2 cut(s)	1412, 4307
BsiHKAI	GWGCWC	6 cut(s)	1425, 2602, 2929, 3319, 3699, 3953
BsiSI	CCGG	4 cut(s)	146, 2239, 3056, 4311
BslI	CCNNNNNNNGG	8 cut(s)	359, 383, 692, 1178, 1763, 1764, 2078, 3062
BsmAI	GTCTC	6 cut(s)	130, 410, 1482, 1883, 2175, 2793
BsmI	GAATGC	1 cut(s)	867
BsnI	GGCC	6 cut(s)	387, 450, 1212, 2745, 2849, 2966
Bsp120I	GGGCCC	1 cut(s)	385
Bsp13I	TCCGGA	1 cut(s)	3055
Bsp1407I	TGTACA	2 cut(s)	1116, 2281
Bsp1720I	GCTNAGC	1 cut(s)	1461
BspANI	GGCC	6 cut(s)	387, 450, 1212, 2745, 2849, 2966
BspCNI	CTCAG	7 cut(s)	1288, 1336, 1453, 1705, 2190, 3538, 4403
BspEI	TCCGGA	1 cut(s)	3055
BspFNI	CGCG	1 cut(s)	1137
BspHI	TCATGA	3 cut(s)	783, 918, 4429
BspLI	GGNNCC	8 cut(s)	375, 387, 1414, 2456, 2527, 2898, 3708, 4309
BspMAI	CTGCAG	3 cut(s)	251, 2437, 2662
BspMI	ACCTGC	3 cut(s)	1918, 3500, 3529
BspPI	GGATC	8 cut(s)	368, 381, 593, 982, 2542, 2891, 2904, 4154
BspT107I	GGYRCC	2 cut(s)	1412, 4307
BsrDI	GCAATG	3 cut(s)	407, 927, 2070
BsrGI	TGTACA	2 cut(s)	1116, 2281
BsrI	ACTGG	9 cut(s)	283, 1050, 1258, 2467, 2518, 2827, 3798, 4366, 4383
BssECI	CCNNGG	5 cut(s)	795, 1408, 1607, 3335, 3674
BssNAI	GTATAC	2 cut(s)	4059, 4292
BssNI	GRCGYC	1 cut(s)	4437
BssSI	CACGAG	1 cut(s)	3740
BssT1I	CCWWGG	3 cut(s)	795, 1607, 3674
Bst1107I	GTATAC	2 cut(s)	4059, 4292
Bst2BI	CACGAG	1 cut(s)	3740
Bst2UI	CCWGG	6 cut(s)	447, 1410, 2394, 3104, 3165, 3336
Bst6I	CTCTTC	5 cut(s)	128, 163, 2044, 3779, 3779
BstACI	GRCGYC	1 cut(s)	4437
BstAUI	TGTACA	2 cut(s)	1116, 2281
BstBAI	YACGTR	1 cut(s)	1153
BstFNI	CGCG	1 cut(s)	1137
BstH2I	RGCGCY	1 cut(s)	1195
BstHHI	GCGC	2 cut(s)	1114, 1194
BstMAI	GTCTC	6 cut(s)	130, 410, 1482, 1883, 2175, 2793
BstNI	CCWGG	6 cut(s)	447, 1410, 2394, 3104, 3165, 3336
BstNSI	RCATGY	6 cut(s)	423, 1684, 1688, 2479, 2788, 2973
BstSCI	CCNGG	7 cut(s)	445, 1408, 2237, 2392, 3102, 3163, 3334
BstSFI	CTRYAG	9 cut(s)	247, 942, 2433, 2658, 2706, 3090, 3189, 3462, 3633
BstSLI	GKGCMC	8 cut(s)	246, 389, 686, 1417, 2072, 2135, 2239, 2602
BstUI	CGCG	1 cut(s)	1137
BstV2I	GAAGAC	3 cut(s)	56, 1211, 3926
BstX2I	RGATCY	4 cut(s)	373, 598, 2534, 2896
BstXI	CCANNNNNNTGG	1 cut(s)	4373
BstYI	RGATCY	4 cut(s)	373, 598, 2534, 2896
BstZ17I	GTATAC	2 cut(s)	4059, 4292
BsuI	GTATCC	2 cut(s)	2746, 3800
BsuRI	GGCC	6 cut(s)	387, 450, 1212, 2745, 2849, 2966
BtsI	GCAGTG	2 cut(s)	244, 1385
BveI	ACCTGC	3 cut(s)	1918, 3500, 3529
CciI	TCATGA	3 cut(s)	783, 918, 4429
CfoI	GCGC	2 cut(s)	1114, 1194
Cfr13I	GGNCC	9 cut(s)	385, 386, 1048, 1210, 1394, 1449, 2455, 2815, 2847
CseI	GACGC	2 cut(s)	4018, 4415
CsiI	ACCWGGT	1 cut(s)	2392
DraI	TTTAAA	2 cut(s)	766, 3082
DrdI	GACNNNNNNGTC	1 cut(s)	2822
DseDI	GACNNNNNNGTC	1 cut(s)	2822
EaeI	YGGCCR	1 cut(s)	448
Eam1104I	CTCTTC	5 cut(s)	128, 163, 2044, 3779, 3779
EarI	CTCTTC	5 cut(s)	128, 163, 2044, 3779, 3779
Ecl136II	GAGCTC	2 cut(s)	2927, 3317
Eco130I	CCWWGG	3 cut(s)	795, 1607, 3674
Eco147I	AGGCCT	1 cut(s)	2745
Eco24I	GRGCYC	4 cut(s)	389, 724, 2929, 3319
Eco47I	GGWCC	5 cut(s)	1048, 1394, 1449, 2455, 2815
Eco53kI	GAGCTC	2 cut(s)	2927, 3317
Eco57I	CTGAAG	5 cut(s)	65, 2061, 3000, 3257, 3942
Eco72I	CACGTG	1 cut(s)	1153
EcoICRI	GAGCTC	2 cut(s)	2927, 3317
EcoO109I	RGGNCCY	2 cut(s)	386, 2815
EcoRII	CCWGG	6 cut(s)	445, 1408, 2392, 3102, 3163, 3334
EcoT14I	CCWWGG	3 cut(s)	795, 1607, 3674
EcoT22I	ATGCAT	1 cut(s)	1686
EcoT38I	GRGCYC	4 cut(s)	389, 724, 2929, 3319
ErhI	CCWWGG	3 cut(s)	795, 1607, 3674
FauI	CCCGC	4 cut(s)	76, 386, 2838, 3283
FauNDI	CATATG	3 cut(s)	822, 3583, 4098
FbaI	TGATCA	1 cut(s)	4216
FblI	GTMKAC	3 cut(s)	901, 4058, 4291
FriOI	GRGCYC	4 cut(s)	389, 724, 2929, 3319
GlaI	GCGC	2 cut(s)	1113, 1193
GsuI	CTGGAG	2 cut(s)	149, 3393
HaeII	RGCGCY	1 cut(s)	1195
HaeIII	GGCC	6 cut(s)	387, 450, 1212, 2745, 2849, 2966
HapII	CCGG	4 cut(s)	146, 2239, 3056, 4311
HgaI	GACGC	2 cut(s)	4018, 4415
HhaI	GCGC	2 cut(s)	1114, 1194
Hin1I	GRCGYC	1 cut(s)	4437
Hin6I	GCGC	2 cut(s)	1112, 1192
HinP1I	GCGC	2 cut(s)	1112, 1192
HincII	GTYRAC	2 cut(s)	2576, 4252
HindII	GTYRAC	2 cut(s)	2576, 4252
HindIII	AAGCTT	2 cut(s)	1561, 3596
HpaII	CCGG	4 cut(s)	146, 2239, 3056, 4311
HphI	GGTGA	8 cut(s)	22, 419, 1263, 1514, 1679, 2318, 3187, 3761
Hpy99I	CGWCG	1 cut(s)	2598
HpyCH4IV	ACGT	7 cut(s)	516, 1092, 1152, 1345, 3832, 3882, 4437
HpySE526I	ACGT	7 cut(s)	516, 1092, 1152, 1345, 3832, 3882, 4437
Hsp92I	GRCGYC	1 cut(s)	4437
HspAI	GCGC	2 cut(s)	1112, 1192
Kpn2I	TCCGGA	1 cut(s)	3055
Ksp22I	TGATCA	1 cut(s)	4216
LmnI	GCTCC	4 cut(s)	2924, 2932, 3314, 3431
LweI	GCATC	9 cut(s)	809, 1576, 1856, 2392, 3277, 3610, 3628, 4092, 4179
MabI	ACCWGGT	1 cut(s)	2392
MaeII	ACGT	7 cut(s)	516, 1092, 1152, 1345, 3832, 3882, 4437
MfeI	CAATTG	4 cut(s)	738, 2265, 3300, 3825
MflI	RGATCY	4 cut(s)	373, 598, 2534, 2896
MlsI	TGGCCA	1 cut(s)	450
MluNI	TGGCCA	1 cut(s)	450
MlyI	GAGTC	5 cut(s)	46, 784, 3046, 3411, 3577
MmeI	TCCRAC	7 cut(s)	351, 472, 838, 1282, 2052, 3149, 3494
Mox20I	TGGCCA	1 cut(s)	450
Mph1103I	ATGCAT	1 cut(s)	1686
MroI	TCCGGA	1 cut(s)	3055
MscI	TGGCCA	1 cut(s)	450
MslI	CAYNNNNRTG	6 cut(s)	932, 1434, 1685, 1861, 4283, 4313
Msp20I	TGGCCA	1 cut(s)	450
MspA1I	CMGCKG	3 cut(s)	43, 2759, 3235
MspI	CCGG	4 cut(s)	146, 2239, 3056, 4311
MspR9I	CCNGG	7 cut(s)	447, 1410, 2239, 2394, 3104, 3165, 3336
MunI	CAATTG	4 cut(s)	738, 2265, 3300, 3825
Mva1269I	GAATGC	1 cut(s)	867
MvaI	CCWGG	6 cut(s)	447, 1410, 2394, 3104, 3165, 3336
MvnI	CGCG	1 cut(s)	1137
NciI	CCSGG	1 cut(s)	2239
NdeI	CATATG	3 cut(s)	822, 3583, 4098
NlaIV	GGNNCC	8 cut(s)	375, 387, 1414, 2456, 2527, 2898, 3708, 4309
NmuCI	GTSAC	8 cut(s)	407, 575, 619, 1502, 1667, 2291, 2345, 3220
NsiI	ATGCAT	1 cut(s)	1686
NspI	RCATGY	6 cut(s)	423, 1684, 1688, 2479, 2788, 2973
OliI	CACNNNNGTG	1 cut(s)	1861
PaeI	GCATGC	1 cut(s)	2479
PagI	TCATGA	3 cut(s)	783, 918, 4429
PaqCI	CACCTGC	1 cut(s)	1918
PceI	AGGCCT	1 cut(s)	2745
PciI	ACATGT	2 cut(s)	419, 2784
PctI	GAATGC	1 cut(s)	867
PfeI	GAWTC	8 cut(s)	791, 1305, 1631, 2215, 2259, 2420, 3154, 4155
PflMI	CCANNNNNTGG	2 cut(s)	1178, 2078
PfoI	TCCNGGA	1 cut(s)	3102
PleI	GAGTC	5 cut(s)	45, 783, 3046, 3410, 3576
PmaCI	CACGTG	1 cut(s)	1153
PmlI	CACGTG	1 cut(s)	1153
PpsI	GAGTC	5 cut(s)	45, 783, 3046, 3410, 3576
Ppu21I	YACGTR	1 cut(s)	1153
PpuMI	RGGWCCY	1 cut(s)	2815
PscI	ACATGT	2 cut(s)	419, 2784
PshBI	ATTAAT	1 cut(s)	807
PsiI	TTATAA	2 cut(s)	1650, 1817
Psp124BI	GAGCTC	2 cut(s)	2929, 3319
Psp5II	RGGWCCY	1 cut(s)	2815
Psp6I	CCWGG	6 cut(s)	445, 1408, 2392, 3102, 3163, 3334
PspCI	CACGTG	1 cut(s)	1153
PspGI	CCWGG	6 cut(s)	445, 1408, 2392, 3102, 3163, 3334
PspN4I	GGNNCC	8 cut(s)	375, 387, 1414, 2456, 2527, 2898, 3708, 4309
PspOMI	GGGCCC	1 cut(s)	385
PspPI	GGNCC	9 cut(s)	385, 386, 1048, 1210, 1394, 1449, 2455, 2815, 2847
PspPPI	RGGWCCY	1 cut(s)	2815
PstI	CTGCAG	3 cut(s)	251, 2437, 2662
PsuI	RGATCY	4 cut(s)	373, 598, 2534, 2896
PvuII	CAGCTG	2 cut(s)	2759, 3235
RseI	CAYNNNNRTG	6 cut(s)	932, 1434, 1685, 1861, 4283, 4313
SacI	GAGCTC	2 cut(s)	2929, 3319
Sau96I	GGNCC	9 cut(s)	385, 386, 1048, 1210, 1394, 1449, 2455, 2815, 2847
ScaI	AGTACT	2 cut(s)	505, 3468
SchI	GAGTC	5 cut(s)	46, 784, 3046, 3411, 3577
ScrFI	CCNGG	7 cut(s)	447, 1410, 2239, 2394, 3104, 3165, 3336
SexAI	ACCWGGT	1 cut(s)	2392
SfaNI	GCATC	9 cut(s)	809, 1576, 1856, 2392, 3277, 3610, 3628, 4092, 4179
SfcI	CTRYAG	9 cut(s)	247, 942, 2433, 2658, 2706, 3090, 3189, 3462, 3633
SinI	GGWCC	5 cut(s)	1048, 1394, 1449, 2455, 2815
SmiMI	CAYNNNNRTG	6 cut(s)	932, 1434, 1685, 1861, 4283, 4313
SmlI	CTYRAG	3 cut(s)	723, 1788, 3470
SmoI	CTYRAG	3 cut(s)	723, 1788, 3470
SphI	GCATGC	1 cut(s)	2479
SseBI	AGGCCT	1 cut(s)	2745
SspI	AATATT	2 cut(s)	589, 1363
SstI	GAGCTC	2 cut(s)	2929, 3319
StuI	AGGCCT	1 cut(s)	2745
StyD4I	CCNGG	7 cut(s)	445, 1408, 2237, 2392, 3102, 3163, 3334
StyI	CCWWGG	3 cut(s)	795, 1607, 3674
TaiI	ACGT	7 cut(s)	519, 1095, 1155, 1348, 3835, 3885, 4440
TaqI	TCGA	4 cut(s)	328, 547, 1708, 2620
TatI	WGTACW	6 cut(s)	503, 647, 1116, 2281, 3466, 3906
TauI	GCSGC	2 cut(s)	1693, 2852
TfiI	GAWTC	8 cut(s)	791, 1305, 1631, 2215, 2259, 2420, 3154, 4155
TseFI	GTSAC	8 cut(s)	407, 575, 619, 1502, 1667, 2291, 2345, 3220
Tsp45I	GTSAC	8 cut(s)	407, 575, 619, 1502, 1667, 2291, 2345, 3220
TspGWI	ACGGA	7 cut(s)	949, 1901, 2084, 2271, 2919, 3514, 4226
Van91I	CCANNNNNTGG	2 cut(s)	1178, 2078
VneI	GTGCAC	1 cut(s)	2598
VpaK11BI	GGWCC	5 cut(s)	1048, 1394, 1449, 2455, 2815
VspI	ATTAAT	1 cut(s)	807
XapI	RAATTY	2 cut(s)	315, 1975
XbaI	TCTAGA	3 cut(s)	2809, 3227, 3247
XceI	RCATGY	6 cut(s)	423, 1684, 1688, 2479, 2788, 2973
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1526, 1932, 3212
XmiI	GTMKAC	3 cut(s)	901, 4058, 4291
ZraI	GACGTC	1 cut(s)	4438
ZrmI	AGTACT	2 cut(s)	505, 3468
Zsp2I	ATGCAT	1 cut(s)	1686

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.