Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0003176

Physical Location & Seq

Genomic Coordinates Forward (+)

35099636 .. 35104039

Gene Structure 4404 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024360

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4404 bp

ATGGGTGAAATCTTGGTCGTCCACGTGGTGTTTGCCCTCTCCAGTTCGCCAGAGTATTTATGTTCTCACAGATTCCAGCCGCAGGCCAAAGTCCCAATCGTGAAAACCCTAACCGGCGGAACCATAGCCGCCGCTGTGAAGAGTTCCGACACCATTGACATCCCCCACCACAAACAGCCCATACCAGCTCCGCCGGACACCAGCTCAAGTCTGCCGGCACTCTCGCCGATCACAACCTCCATGAATACCCAACGGGCCCCAGTGGTCTTCACAAAACAGCCCTACATTGAACAAGAAGACCACCGGAGAATAGCCCAATGGGAATTCAACGCTCTTTCCCAGTCGGAGATCGCCAAAATGGCGGAGGCCCAAATCTTCGAAGGCAAGTACTACGATTCCGCCACCGGAAAACCCATCCAAGGCGGGTTGCTGGACCCCAGATTGGGCCCTGCGAATCGCCACAGTGGCAACTGCGCCACTTGCTCTGCAAAACTCTCCGACTGCCCGGGGCACTTTGGGTATTTCATGCTTTCCCTCCCTGTTTTCAATGTTGGGTATTTGAATGTGGTTGTGGACATTTTGAAGTGCATCTGTAAAATGTGCTCTAGAATTCTTTTGGATGAGAAACTGAGAAGCAAGAAGGAGTTGATGAAGGACATGAAATCGATAGTGAAGGTGTGTGTTAAGCCCTTGACGAATCATAGAGCCGTGGAGTGCTCCCATTGTGGGTATACAAATGGCAATGTGAGAAGGGTTCCGGGGGATGTGGGAATTCAACACGATCGTTCGGAACGAACTGTTAATGGTGTTGTGGTAGATGAGTATGAGTCGGCGATACTTGACAAGGGAGTCATTAATATGCAACAAAATTTTTTGCTTGACCCTGCTAGAGTTTATTCTCTGTTTAGGAAGATGCTTGATGTGGATTGCCGGTTTCTGTGTCTTAAAACTCGACCGGAGAATCTCCTGTTGACTTGCATTCTTGTGCCGCCGATAGCTATTCGGCCTTCGGTTCTTGTAGGAGGATCACAGAGTAATGAGGATGATGTGACTGTGAGGCTGAAGAGGATTATTCAAGCAAATGCGAAACTTCGACTGCAGCTATCATCTGCAGTAAGTTGCTCTGCTGTGTCATTGTCTTGTTGGGAGGAGCTTCAGATTGAGGTTGCACAGTATATAAATGGTGAGGCTCGTGTACACCTTCCGCCGAGCTTGAGAGCTCAACAACCTACCAAGCCATTGAGTGGTTTGGTGCAGCGCCTCAAAGGGAAGACGGGGCGGTTTCGTGGTACTTTGAGTGGGAAGCGTGTTGAGTATACTGGGAGAAGTGTTATATCACCGGACCCTAATCTGAAAATTACTGAGGTTGGAATCCCTATAGAAATGGCTACAATTTTGAGTTATCCAGAACGTGTTTCCAAGCATAATATTGACAAGTTGAGGCTGTGTGTCAGTAATGGGCCTTATAAGTACCCAGGTGCTAAAATGTTGAGAAATCATGACGGCTCTGAGTTCACCTTGAAGGTTAACAGACAGGGCCGTGCTCGTGGACTCAAATGTGGTGACATAGTTGATCGGCATTTAGAAGATGGGGACATTGTTCTTTTCAACAGGCAACCAAGCTTGCATCGGATGTCTGTCATGTGTCATAGGGCAAGGATTATGCCACATTCGAGGACATTGAGGTTTAATGAATCTGTTTGCAACCCGTATAATGCTGATTTTGATGGTGATGAAATGAATATTCATGTCCCACAAACTGAGGAGGCTCGTGCTGAGGCGCTTATGTTGATGGGGGTGCATAATAATCTATGCACTCCGAAAAATGGAGAGATTTTGGTTGCTTCCACTCAAGATTTTCTAACTTCTTCTTTCCTTATAACAAGGAAGGACACATTTTATGACCGTGCATCTTTTTCACTCCTGTGTTCATATATGGTTGATGGCAAGGCTTCTGTTGATTTGCCAACACCTGCTGTAGTTAAGCCAGTAGAGCTCTGGACAGGTAAGCAGCTATTTAATGTTTTAGTACGCCCAAACTCAAATGTGAGAGTCTATCTAAATCTTATTGTTAAGGAGAAATCCTACACCTCTGCCAGGGCCGAGGATGGAAGAGAAACAGAAGCAATGTGCCCAAACGATGGTTTTGTCTATTTTCGCAACAGTGAGCTATTATCTGGGCAACTTGGGAAGGCTACTCTAGGAAATGGCAATAAGGATGGACTTTACTCTGTTCTTCTCAGAGACTATGAAGCTCATGCTGCTGCTTCTTGCATGAATCGCCTAGCTAAATTAAGTGCACGGTGGATTGGTAATCATGGATTCTCGATTGGGCTTAGTGATGTACAACCAAGTGACAATTTGTATGATGAGAAAGAAAAAATCATCAAGGAAGGTTATGGCAAATGTGACAGCCAGATAGACTTGTATAATCAACGCAAGCTGGACTCACTTGAATCACTTGAGTCTGGGATTTCTGAGGTGTTAAATAAGATTAGAGATCAAACTGGAAAGCTGTGCATGCAAAAGCTACATTGGAGAAACAGTCCCTTGATCATGTCCCAGTGTGGCTCCAAGGGATCTGCTATCAATATTAGCCAGATGGTTGCTTGTGTTGGTCAGCAATCTGTTGGTGGTCGCCGTGCACCTGATGGTTTCATAGATCGAAGCCTTCCACACTTCCCTAGGAATGCAAAAATGCCTGCAGCTAAAGGCTTTGTAGCTAATTCCTTTTATAGTGGCCTGACACCTACAGAGTTTTTCTTTCATACAATGGGAGGGCGAGAGGGACTTGTTGATACAGCAGTGAAAACTGCTGAAACTGGCTACATGTCACGTAGATTGTCAAAGGTTTTAGAAGATCTCGCCATCCACTATGATAAAACAGTTCGGGTTGCCAATGGAGGTATAGTTCAGTTCTGTTATGGGGATGACGGTATGGACCCTGCTATGATGGAAGGGAAAAGTGGAGCACCGATAGACTTATTTCGAATGTTTTTGAAAGCCAAGGCCACATGCCCTGCTGAAGAAACTGATAGCAGCCTGTCTCCTGAACAAGTGTCTGAAGTAGTGAGAATCAGGCTTTCAGAACAAGATATGATTCCTGAAGATGGTTTGTCTATGTGTTTTAAAAGTTCTATAGAGAAATTCCTGGATGAATATGCAAAAACACAAAGAAAAACACAGGAGACTTTTTTGTTGAATCAAAGTTCTGGTTGGGAGGTGAAGTCTGCTATTCTGGAGAATATTGTCAAAAATATATCTGGTGTCACTTCTAGACAGCTGGAGGTTTTTCTAGATACTTGTATATCTCGTTATCATTCAAAAAGAATTGATGCTGGAACGGCAATTGGAGCAATTGGAGCTCAAAGTATTGGCGAGCCTGTGACACAGATGACACTGAAAACCTTTCACTTTGCTGGAGTTGCAAGCATGAACGTTACACTTGGAGTCCCTCGGATAAAGGAGATAATAGATGGAGCAAAAAATATCAAGACACCCATCATCACTACAGTACTCGAGTGTGATAATAATGCGATGATTGCACGGATGGTGGCAGGTCGGATTGGGAGCACAAATTTAGGGCAGGTGGCTAAGAGTATAGAGATCCTAATGAGTCCAAGACAGGCATGCATAGTCATCAAGCTTGATATGGTCATGATTCGAGATGCACAGTTGTCTATAGATGTTGATGTTGTGAAAGAATCAATTTTAAAAGTTCCGAGAATCAAACTGAAGCAAGAGAATATTCGAGTTCCAGATAACGAAACTTTGGAAGTGTTTTCTCAGGACAAGGATAGACGCAGACTTTATTTTGAGCTCTACAGATTAAAGAGTATCCTCCCCAATGTTACAGTAAGGGGCATAACGACAGTTCAGCGTGTTGTCATCGATGAAAGTGATGTGAAAAAGGATAAAGAAGATGTTCAAGTTAAAGGTGCAGATGTTGTTGGTAAGAAGTACAGGTTGTTTGCTGAAGGCAAGGGACTTCTGGCAGTAATGAGCACTGAAGGAGTAGATGGTTGTAAAACCACAAGTAATGACATTATTGAAGTGCAGCAGACACTTGGGATTGAAGCTGCGAGAATTTCTATCATAGAGGAGATAAAGTATACTATGAAGCAACATGGAATGAACATAGATGATCGCCATATGATGCTTTTAGCGGATGCAATGACTTCCAGGGGTCAAGTTCTTGGAATGACGAGATTTGGCCTTCAAAAGATGGACAAAAGTGTTTTAATGGGTGCTTCATTTGAGCGAACAGATGATCATCTGTTTAATGCTTGTGTGAAGGGGGCAGTAGACAAAATTGAAGGAGTAAGTGAAAGCATAATCATGGGTATACCAATGCCGATAGGAACTGGAATGTTCAAAGTTAAACACATCCTCCCTACTACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002376 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0046483 GO:0048518 GO:0050789 GO:0050896 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1468

Amino Acids

162.57

Weight (kDa)

8.73

Isoelectric Point (pI)

43.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	102 - 432	1.1e-67	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	434 - 600	1.1e-71	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	603 - 782	2.3e-37	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	812 - 907	5.4e-28	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	914 - 1413	1.6e-85	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60040 AT5G60040
fragaria_vesca	FvH4_3g34290 FvH4_3g34290 FvH4_3g34290 FvH4_6g15631
malus_domestica	MD03G1106800.v1.1 MD09G1157000.v1.1
prunus_persica	Prupe.6G089600_v2.0.a1 Prupe.6G089600_v2.0.a1
pyrus_communis	pycom03g08620 pycom09g07570
rosa_chinensis	RchiOBHm_Chr3g0469301 RchiOBHm_Chr3g0469321 RchiOBHm_Chr5g0061861
rosa_laevigata	RLG00000024360 RLG00000035511
rosa_multiflora	Rmu_sc0004134.1_g000001 Rmu_sc0011641.1_g000013 Rmu_sc0011641.1_g000015 Rmu_sc0019271.1_g000001 Rmu_sc0022191.1_g000001
rosa_roxburghii	Rroxscaffold_1G00018870 Rroxscaffold_6G00411990 Rroxscaffold_6G00412000
rosa_rugosa	Rorug03G0100400 Rorug05G0344900 Rorug05G0345000
rosa_samantha	Rh3AG149800 Rh3BG173400 Rh3CG164500 Rh3DG202000 Rh5AG405500 Rh5BG418300 Rh5CG442800 Rh5DG432400
rosa_wichuraiana	Rw5G038070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1467, 1880
AarI	CACCTGC	2 cut(s)	1981, 3577
AasI	GACNNNNNNGTC	1 cut(s)	848
Acc36I	ACCTGC	3 cut(s)	1981, 3548, 3577
AccB7I	CCANNNNNTGG	2 cut(s)	1244, 2141
AccI	GTMKAC	5 cut(s)	731, 1316, 4112, 4305, 4345
AclI	AACGTT	1 cut(s)	3438
AclWI	GGATC	3 cut(s)	1033, 2587, 3601
AcsI	RAATTY	7 cut(s)	323, 609, 771, 868, 3146, 3577, 4086
AcuI	CTGAAG	8 cut(s)	1082, 1139, 3045, 3084, 3126, 3755, 3996, 4029
AcvI	CACGTG	1 cut(s)	25
AdeI	CACNNNGTG	1 cut(s)	28
AfaI	GTAC	8 cut(s)	389, 1197, 1291, 1472, 2031, 2346, 3516, 3962
AflIII	ACRYGT	2 cut(s)	1411, 2829
AjnI	CCWGG	4 cut(s)	1474, 2096, 3150, 4181
AleI	CACNNNNGTG	1 cut(s)	1924
AloI	GAACNNNNNNTCC	2 cut(s)	2895, 2927
Alw26I	GTCTC	3 cut(s)	2238, 3051, 3182
Alw44I	GTGCAC	2 cut(s)	2298, 2643
AlwI	GGATC	3 cut(s)	1033, 2587, 3601
Ama87I	CYCGRG	2 cut(s)	505, 3518
ApaI	GGGCCC	2 cut(s)	259, 449
ApaLI	GTGCAC	2 cut(s)	2298, 2643
ApeKI	GCWGC	9 cut(s)	1099, 1255, 2011, 2261, 2264, 2705, 3039, 4057, 4079
ApoI	RAATTY	7 cut(s)	323, 609, 771, 868, 3146, 3577, 4086
AseI	ATTAAT	1 cut(s)	855
Asp700I	GAANNNNTTC	1 cut(s)	3924
AspA2I	CCTAGG	1 cut(s)	2684
AspLEI	GCGC	3 cut(s)	476, 1260, 1783
AsuC2I	CCSGG	3 cut(s)	506, 507, 759
AsuHPI	GGTGA	7 cut(s)	17, 1196, 1329, 1507, 1574, 1742, 3235
AsuII	TTCGAA	2 cut(s)	378, 2989
AvaI	CYCGRG	2 cut(s)	505, 3518
AvaII	GGWCC	3 cut(s)	433, 1342, 2941
AvrII	CCTAGG	1 cut(s)	2684
BaeGI	GKGCMC	6 cut(s)	259, 449, 513, 2135, 2302, 2647
BaeI	ACNNNNGTAYC	2 cut(s)	2790, 2823
BanII	GRGCYC	6 cut(s)	259, 449, 1222, 1998, 3367, 3822
BauI	CACGAG	3 cut(s)	1191, 1545, 1770
BbrPI	CACGTG	1 cut(s)	25
BbsI	GAAGAC	3 cut(s)	259, 303, 1277
BbvCI	CCTCAGC	1 cut(s)	1776
BbvI	GCAGC	9 cut(s)	1111, 1267, 2023, 2248, 2251, 2717, 3051, 4066, 4069
BceAI	ACGGC	5 cut(s)	692, 1519, 1524, 2625, 3360
BciT130I	CCWGG	4 cut(s)	1476, 2098, 3152, 4183
BciVI	GTATCC	1 cut(s)	3848
BclI	TGATCA	2 cut(s)	2553, 4270
BcnI	CCSGG	3 cut(s)	506, 507, 759
BcoDI	GTCTC	3 cut(s)	2238, 3051, 3182
BfaI	CTAG	7 cut(s)	606, 888, 2201, 2285, 2685, 3276, 3296
BfoI	RGCGCY	2 cut(s)	1261, 1784
BfuAI	ACCTGC	3 cut(s)	1981, 3548, 3577
BfuI	GTATCC	1 cut(s)	3848
BglI	GCCNNNNNGGC	2 cut(s)	359, 465
BglII	AGATCT	1 cut(s)	2860
BlnI	CCTAGG	1 cut(s)	2684
BmcAI	AGTACT	2 cut(s)	389, 3516
Bme1390I	CCNGG	7 cut(s)	506, 507, 759, 1476, 2098, 3152, 4183
Bme18I	GGWCC	3 cut(s)	433, 1342, 2941
BmeT110I	CYCGRG	2 cut(s)	505, 3518
BmiI	GGNNCC	9 cut(s)	121, 257, 258, 435, 447, 756, 1344, 2572, 2943
BmrFI	CCNGG	7 cut(s)	506, 507, 759, 1476, 2098, 3152, 4183
BmrI	ACTGGG	4 cut(s)	254, 334, 1329, 2557
BmsI	GCATC	8 cut(s)	597, 903, 1636, 1919, 3325, 3658, 4146, 4159
BmuI	ACTGGG	4 cut(s)	254, 334, 1329, 2557
BpiI	GAAGAC	3 cut(s)	259, 303, 1277
BpmI	CTGGAG	4 cut(s)	25, 3260, 3305, 3441
Bpu10I	CCTNAGC	1 cut(s)	1776
Bpu14I	TTCGAA	2 cut(s)	378, 2989
BpuEI	CTTGAG	4 cut(s)	190, 1234, 1836, 2483
BpuMI	CCSGG	3 cut(s)	506, 507, 759
Bsa29I	ATCGAT	2 cut(s)	665, 3891
BsaAI	YACGTR	2 cut(s)	25, 2837
BsaBI	GATNNNNATC	1 cut(s)	4272
BsaWI	WCCGGW	4 cut(s)	303, 404, 955, 1339
Bse118I	RCCGGY	3 cut(s)	113, 214, 930
Bse1I	ACTGG	9 cut(s)	42, 260, 340, 1324, 1988, 2512, 2563, 2827, 4369
Bse3DI	GCAATG	3 cut(s)	748, 2133, 4179
Bse8I	GATNNNNATC	1 cut(s)	4272
BseBI	CCWGG	4 cut(s)	1476, 2098, 3152, 4183
BseCI	ATCGAT	2 cut(s)	665, 3891
BseJI	GATNNNNATC	1 cut(s)	4272
BseMI	GCAATG	3 cut(s)	748, 2133, 4179
BseMII	CTCAG	8 cut(s)	620, 1353, 1500, 1752, 1767, 2254, 2469, 3800
BseNI	ACTGG	9 cut(s)	42, 260, 340, 1324, 1988, 2512, 2563, 2827, 4369
BseRI	GAGGAG	3 cut(s)	1163, 1778, 4115
BseSI	GKGCMC	6 cut(s)	259, 449, 513, 2135, 2302, 2647
BseXI	GCAGC	9 cut(s)	1111, 1267, 2023, 2248, 2251, 2717, 3051, 4066, 4069
BsgI	GTGCAG	3 cut(s)	1274, 3960, 4076
Bsh1285I	CGRYCG	2 cut(s)	784, 956
BshVI	ATCGAT	2 cut(s)	665, 3891
BsiEI	CGRYCG	2 cut(s)	784, 956
BsiHKCI	CYCGRG	2 cut(s)	505, 3518
BslFI	GGGAC	8 cut(s)	77, 1607, 1736, 2532, 2545, 2802, 3437, 3999
BsmAI	GTCTC	3 cut(s)	2238, 3051, 3182
BsmFI	GGGAC	8 cut(s)	77, 1607, 1736, 2532, 2545, 2802, 3437, 3999
BsmI	GAATGC	2 cut(s)	978, 2695
BsoBI	CYCGRG	2 cut(s)	505, 3518
Bsp119I	TTCGAA	2 cut(s)	378, 2989
Bsp120I	GGGCCC	2 cut(s)	255, 445
Bsp1407I	TGTACA	2 cut(s)	1195, 2344
BspCNI	CTCAG	8 cut(s)	621, 1354, 1501, 1753, 1768, 2253, 2470, 3799
BspDI	ATCGAT	2 cut(s)	665, 3891
BspHI	TCATGA	2 cut(s)	1498, 3657
BspLI	GGNNCC	9 cut(s)	121, 257, 258, 435, 447, 756, 1344, 2572, 2943
BspMAI	CTGCAG	3 cut(s)	1101, 1114, 2707
BspMI	ACCTGC	3 cut(s)	1981, 3548, 3577
BspPI	GGATC	3 cut(s)	1033, 2587, 3601
BspT104I	TTCGAA	2 cut(s)	378, 2989
BsrDI	GCAATG	3 cut(s)	748, 2133, 4179
BsrFI	RCCGGY	3 cut(s)	113, 214, 930
BsrGI	TGTACA	2 cut(s)	1195, 2344
BsrI	ACTGG	9 cut(s)	42, 260, 340, 1324, 1988, 2512, 2563, 2827, 4369
BssAI	RCCGGY	3 cut(s)	113, 214, 930
BssNAI	GTATAC	4 cut(s)	732, 1317, 4113, 4346
BssSI	CACGAG	3 cut(s)	1191, 1545, 1770
BssT1I	CCWWGG	4 cut(s)	418, 2574, 2684, 3006
Bst1107I	GTATAC	4 cut(s)	732, 1317, 4113, 4346
Bst2BI	CACGAG	3 cut(s)	1191, 1545, 1770
Bst2UI	CCWGG	4 cut(s)	1476, 2098, 3152, 4183
Bst6I	CTCTTC	3 cut(s)	134, 1058, 2107
BstAUI	TGTACA	2 cut(s)	1195, 2344
BstBAI	YACGTR	2 cut(s)	25, 2837
BstBI	TTCGAA	2 cut(s)	378, 2989
BstC8I	GCNNGC	9 cut(s)	84, 216, 1625, 2441, 2522, 2703, 3380, 3430, 3631
BstDSI	CCRYGG	1 cut(s)	708
BstENI	CCTNNNNNAGG	1 cut(s)	2095
BstH2I	RGCGCY	2 cut(s)	1261, 1784
BstHHI	GCGC	3 cut(s)	476, 1260, 1783
BstMAI	GTCTC	3 cut(s)	2238, 3051, 3182
BstMCI	CGRYCG	2 cut(s)	784, 956
BstNI	CCWGG	4 cut(s)	1476, 2098, 3152, 4183
BstNSI	RCATGY	4 cut(s)	2524, 2833, 3018, 3633
BstSCI	CCNGG	7 cut(s)	504, 505, 757, 1474, 2096, 3150, 4181
BstSLI	GKGCMC	6 cut(s)	259, 449, 513, 2135, 2302, 2647
BstV1I	GCAGC	9 cut(s)	1111, 1267, 2023, 2248, 2251, 2717, 3051, 4066, 4069
BstV2I	GAAGAC	3 cut(s)	259, 303, 1277
BstX2I	RGATCY	3 cut(s)	2579, 2860, 3606
BstYI	RGATCY	3 cut(s)	2579, 2860, 3606
BstZ17I	GTATAC	4 cut(s)	732, 1317, 4113, 4346
Bsu15I	ATCGAT	2 cut(s)	665, 3891
BsuI	GTATCC	1 cut(s)	3848
BsuTUI	ATCGAT	2 cut(s)	665, 3891
BtgI	CCRYGG	1 cut(s)	708
BtgZI	GCGATG	1 cut(s)	3551
BtsI	GCAGTG	1 cut(s)	2811
BtsIMutI	CAGTG	7 cut(s)	267, 469, 2170, 2570, 2811, 3398, 4005
BveI	ACCTGC	3 cut(s)	1981, 3548, 3577
Cac8I	GCNNGC	9 cut(s)	84, 216, 1625, 2441, 2522, 2703, 3380, 3430, 3631
CciI	TCATGA	2 cut(s)	1498, 3657
CfoI	GCGC	3 cut(s)	476, 1260, 1783
Cfr10I	RCCGGY	3 cut(s)	113, 214, 930
Cfr9I	CCCGGG	1 cut(s)	505
ClaI	ATCGAT	2 cut(s)	665, 3891
CseI	GACGC	1 cut(s)	3810
Csp6I	GTAC	8 cut(s)	388, 1196, 1290, 1471, 2030, 2345, 3515, 3961
CviQI	GTAC	8 cut(s)	388, 1196, 1290, 1471, 2030, 2345, 3515, 3961
DraI	TTTAAA	2 cut(s)	3130, 3714
DraIII	CACNNNGTG	1 cut(s)	28
DrdI	GACNNNNNNGTC	1 cut(s)	848
DseDI	GACNNNNNNGTC	1 cut(s)	848
Eam1104I	CTCTTC	3 cut(s)	134, 1058, 2107
EarI	CTCTTC	3 cut(s)	134, 1058, 2107
EciI	GGCGGA	5 cut(s)	132, 180, 377, 388, 1194
Ecl136II	GAGCTC	4 cut(s)	1220, 1996, 3365, 3820
Eco130I	CCWWGG	4 cut(s)	418, 2574, 2684, 3006
Eco24I	GRGCYC	6 cut(s)	259, 449, 1222, 1998, 3367, 3822
Eco47I	GGWCC	3 cut(s)	433, 1342, 2941
Eco53kI	GAGCTC	4 cut(s)	1220, 1996, 3365, 3820
Eco57I	CTGAAG	8 cut(s)	1082, 1139, 3045, 3084, 3126, 3755, 3996, 4029
Eco72I	CACGTG	1 cut(s)	25
Eco88I	CYCGRG	2 cut(s)	505, 3518
EcoICRI	GAGCTC	4 cut(s)	1220, 1996, 3365, 3820
EcoNI	CCTNNNNNAGG	1 cut(s)	2095
EcoO109I	RGGNCCY	2 cut(s)	256, 446
EcoRI	GAATTC	3 cut(s)	323, 609, 771
EcoRII	CCWGG	4 cut(s)	1474, 2096, 3150, 4181
EcoT14I	CCWWGG	4 cut(s)	418, 2574, 2684, 3006
EcoT22I	ATGCAT	1 cut(s)	3635
EcoT38I	GRGCYC	6 cut(s)	259, 449, 1222, 1998, 3367, 3822
ErhI	CCWWGG	4 cut(s)	418, 2574, 2684, 3006
FaqI	GGGAC	8 cut(s)	77, 1607, 1736, 2532, 2545, 2802, 3437, 3999
FauI	CCCGC	1 cut(s)	416
FauNDI	CATATG	1 cut(s)	4152
FbaI	TGATCA	2 cut(s)	2553, 4270
FblI	GTMKAC	5 cut(s)	731, 1316, 4112, 4305, 4345
FriOI	GRGCYC	6 cut(s)	259, 449, 1222, 1998, 3367, 3822
FspBI	CTAG	7 cut(s)	606, 888, 2201, 2285, 2685, 3276, 3296
GlaI	GCGC	3 cut(s)	475, 1259, 1782
GsuI	CTGGAG	4 cut(s)	25, 3260, 3305, 3441
HaeII	RGCGCY	2 cut(s)	1261, 1784
HgaI	GACGC	1 cut(s)	3810
HhaI	GCGC	3 cut(s)	476, 1260, 1783
Hin6I	GCGC	3 cut(s)	474, 1258, 1781
HinP1I	GCGC	3 cut(s)	474, 1258, 1781
HincII	GTYRAC	2 cut(s)	972, 1528
HindII	GTYRAC	2 cut(s)	972, 1528
HindIII	AAGCTT	2 cut(s)	1621, 3644
HpaI	GTTAAC	1 cut(s)	1528
HphI	GGTGA	7 cut(s)	17, 1196, 1329, 1507, 1574, 1742, 3235
HpyCH4IV	ACGT	4 cut(s)	24, 1411, 2836, 3438
HpySE526I	ACGT	4 cut(s)	24, 1411, 2836, 3438
HspAI	GCGC	3 cut(s)	474, 1258, 1781
KroI	GCCGGC	1 cut(s)	214
KroNI	GCCGGC	1 cut(s)	216
Ksp22I	TGATCA	2 cut(s)	2553, 4270
KspAI	GTTAAC	1 cut(s)	1528
LmnI	GCTCC	9 cut(s)	193, 722, 1150, 2576, 2969, 3353, 3362, 3479, 3570
Lsp1109I	GCAGC	9 cut(s)	1111, 1267, 2023, 2248, 2251, 2717, 3051, 4066, 4069
LweI	GCATC	8 cut(s)	597, 903, 1636, 1919, 3325, 3658, 4146, 4159
MaeI	CTAG	7 cut(s)	606, 888, 2201, 2285, 2685, 3276, 3296
MaeII	ACGT	4 cut(s)	24, 1411, 2836, 3438
MaeIII	GTNAC	9 cut(s)	1048, 1562, 2354, 2408, 2832, 3268, 3385, 3439, 3850
MfeI	CAATTG	2 cut(s)	3348, 3357
MflI	RGATCY	3 cut(s)	2579, 2860, 3606
MlyI	GAGTC	8 cut(s)	836, 858, 1545, 2061, 2441, 2474, 3459, 3625
MmeI	TCCRAC	5 cut(s)	171, 324, 522, 1348, 3542
Mph1103I	ATGCAT	1 cut(s)	3635
MroNI	GCCGGC	1 cut(s)	214
MroXI	GAANNNNTTC	1 cut(s)	3924
MslI	CAYNNNNRTG	4 cut(s)	857, 983, 1924, 4337
MspA1I	CMGCKG	2 cut(s)	134, 3283
MspR9I	CCNGG	7 cut(s)	506, 507, 759, 1476, 2098, 3152, 4183
MunI	CAATTG	2 cut(s)	3348, 3357
Mva1269I	GAATGC	2 cut(s)	978, 2695
MvaI	CCWGG	4 cut(s)	1476, 2098, 3152, 4183
NaeI	GCCGGC	1 cut(s)	216
NciI	CCSGG	3 cut(s)	506, 507, 759
NdeI	CATATG	1 cut(s)	4152
NgoMIV	GCCGGC	1 cut(s)	214
NlaIV	GGNNCC	9 cut(s)	121, 257, 258, 435, 447, 756, 1344, 2572, 2943
NmeAIII	GCCGAG	2 cut(s)	1233, 2128
NmuCI	GTSAC	7 cut(s)	1048, 1562, 2354, 2408, 2832, 3268, 3385
NsiI	ATGCAT	1 cut(s)	3635
NspI	RCATGY	4 cut(s)	2524, 2833, 3018, 3633
NspV	TTCGAA	2 cut(s)	378, 2989
OliI	CACNNNNGTG	1 cut(s)	1924
PaeI	GCATGC	2 cut(s)	2524, 3633
PaeR7I	CTCGAG	1 cut(s)	3518
PagI	TCATGA	2 cut(s)	1498, 3657
PaqCI	CACCTGC	2 cut(s)	1981, 3577
PciI	ACATGT	1 cut(s)	2829
PcsI	WCGNNNNNNNCGW	1 cut(s)	790
PctI	GAATGC	2 cut(s)	978, 2695
PdiI	GCCGGC	1 cut(s)	216
PdmI	GAANNNNTTC	1 cut(s)	3924
PflMI	CCANNNNNTGG	2 cut(s)	1244, 2141
PfoI	TCCNGGA	1 cut(s)	3150
Ple19I	CGATCG	1 cut(s)	784
PleI	GAGTC	8 cut(s)	835, 857, 1545, 2060, 2441, 2473, 3458, 3624
PmaCI	CACGTG	1 cut(s)	25
PmlI	CACGTG	1 cut(s)	25
PpsI	GAGTC	8 cut(s)	835, 857, 1545, 2060, 2441, 2473, 3458, 3624
Ppu21I	YACGTR	2 cut(s)	25, 2837
PscI	ACATGT	1 cut(s)	2829
PshBI	ATTAAT	1 cut(s)	855
PsiI	TTATAA	2 cut(s)	1467, 1880
Psp124BI	GAGCTC	4 cut(s)	1222, 1998, 3367, 3822
Psp1406I	AACGTT	1 cut(s)	3438
Psp6I	CCWGG	4 cut(s)	1474, 2096, 3150, 4181
PspCI	CACGTG	1 cut(s)	25
PspGI	CCWGG	4 cut(s)	1474, 2096, 3150, 4181
PspN4I	GGNNCC	9 cut(s)	121, 257, 258, 435, 447, 756, 1344, 2572, 2943
PspOMI	GGGCCC	2 cut(s)	255, 445
PspXI	VCTCGAGB	1 cut(s)	3518
PstI	CTGCAG	3 cut(s)	1101, 1114, 2707
PsuI	RGATCY	3 cut(s)	2579, 2860, 3606
PvuI	CGATCG	1 cut(s)	784
PvuII	CAGCTG	1 cut(s)	3283
RsaI	GTAC	8 cut(s)	389, 1197, 1291, 1472, 2031, 2346, 3516, 3962
RsaNI	GTAC	8 cut(s)	388, 1196, 1290, 1471, 2030, 2345, 3515, 3961
RseI	CAYNNNNRTG	4 cut(s)	857, 983, 1924, 4337
SacI	GAGCTC	4 cut(s)	1222, 1998, 3367, 3822
ScaI	AGTACT	2 cut(s)	389, 3516
SchI	GAGTC	8 cut(s)	836, 858, 1545, 2061, 2441, 2474, 3459, 3625
ScrFI	CCNGG	7 cut(s)	506, 507, 759, 1476, 2098, 3152, 4183
SfaNI	GCATC	8 cut(s)	597, 903, 1636, 1919, 3325, 3658, 4146, 4159
Sfr274I	CTCGAG	1 cut(s)	3518
SfuI	TTCGAA	2 cut(s)	378, 2989
SinI	GGWCC	3 cut(s)	433, 1342, 2941
SlaI	CTCGAG	1 cut(s)	3518
SmaI	CCCGGG	1 cut(s)	507
SmiMI	CAYNNNNRTG	4 cut(s)	857, 983, 1924, 4337
SmlI	CTYRAG	5 cut(s)	205, 1213, 1851, 2462, 3518
SmoI	CTYRAG	5 cut(s)	205, 1213, 1851, 2462, 3518
SphI	GCATGC	2 cut(s)	2524, 3633
SspI	AATATT	5 cut(s)	1429, 1744, 2593, 3247, 3748
SspMI	CTAG	7 cut(s)	606, 888, 2201, 2285, 2685, 3276, 3296
SstI	GAGCTC	4 cut(s)	1222, 1998, 3367, 3822
StyD4I	CCNGG	7 cut(s)	504, 505, 757, 1474, 2096, 3150, 4181
StyI	CCWWGG	4 cut(s)	418, 2574, 2684, 3006
TaiI	ACGT	4 cut(s)	27, 1414, 2839, 3441
TatI	WGTACW	5 cut(s)	387, 1195, 2344, 3514, 3960
TauI	GCSGC	4 cut(s)	82, 131, 134, 991
TscAI	CASTG	7 cut(s)	267, 469, 2170, 2570, 2811, 3405, 4012
TseFI	GTSAC	7 cut(s)	1048, 1562, 2354, 2408, 2832, 3268, 3385
TseI	GCWGC	9 cut(s)	1099, 1255, 2011, 2261, 2264, 2705, 3039, 4057, 4079
Tsp45I	GTSAC	7 cut(s)	1048, 1562, 2354, 2408, 2832, 3268, 3385
TspGWI	ACGGA	1 cut(s)	3562
TspMI	CCCGGG	1 cut(s)	505
TspRI	CASTG	7 cut(s)	267, 469, 2170, 2570, 2811, 3405, 4012
Van91I	CCANNNNNTGG	2 cut(s)	1244, 2141
VneI	GTGCAC	2 cut(s)	2298, 2643
VpaK11BI	GGWCC	3 cut(s)	433, 1342, 2941
VspI	ATTAAT	1 cut(s)	855
XagI	CCTNNNNNAGG	1 cut(s)	2095
XapI	RAATTY	7 cut(s)	323, 609, 771, 868, 3146, 3577, 4086
XbaI	TCTAGA	3 cut(s)	605, 3275, 3295
XceI	RCATGY	4 cut(s)	2524, 2833, 3018, 3633
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1995
XhoI	CTCGAG	1 cut(s)	3518
XmaI	CCCGGG	1 cut(s)	505
XmaJI	CCTAGG	1 cut(s)	2684
XmiI	GTMKAC	5 cut(s)	731, 1316, 4112, 4305, 4345
XmnI	GAANNNNTTC	1 cut(s)	3924
XspI	CTAG	7 cut(s)	606, 888, 2201, 2285, 2685, 3276, 3296
ZrmI	AGTACT	2 cut(s)	389, 3516
Zsp2I	ATGCAT	1 cut(s)	3635

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.