Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0003176

Physical Location & Seq

Genomic Coordinates Reverse (-)

67777495 .. 67802289

Gene Structure 24795 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG418300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4446 bp

ATGAAAGAGCTGAATAAATTCAAGATAACCTATGAATATGATGATGAAGAGCCAGAGTCCTCCTACCAAATCATCTATCATCGAGATGATTTGGAGAAGTTACAAAAATTGGGGGATGAGAAGAAGGAGGAGCATAAGCAAGGAGAAAGAAAGGGGCAAAAGGAAGGAGAAAGAAAGGATAACAAGGAAGGAGATAGAAAGGCCCCTTCAACCTTCAACCTTGAACCCTCCCTCTTTGCTTTCTGCGTGGAGAAGAGACTTCTACCGGAAAAGATGATGAATAAAGTCCAAGCCAAAACATTAGCCTTCACAAAAGAGCCTTTCATTGAAGAAGAAGGGCACTGCAGAATAGCACAATGGCAATTCAATGCTCTGTCCCAGTCAGAGATCAGCAAAATGGCTGCAACTCAAATTTTTCTGGGTCGATATTACGATAGCAATCAAAAACCAATCAAAGGAGGCTTGTTGGATCCCCGCTTGGGCCCTGCAAATAGGCAATGCGGTGACTGTGAGACATGTGGTGCGAAACTTTCAGATTGTCCTGGCCACTTTGGATATTTGGTGCTTGCTGTCCCTGTATTCAATGTTGGATATTTAAGTACTGTTGTGGACGTTTTGAAGTGCATTTGTAAGTCATGCTCTCGAATAATTTTGGATGACAAATCACGCATTCGCTTTTTGAATATTGTGAGAGATCCCAAAAAGGATAAAAGCGTGACAATGAAATCAATAGTGAAGAAGTGTACTAGCATGGCAAGCAATAATAGATCAGTGAAGTGCCCGAAATGTGGATATATGAATGGTTCAGTGAAGAGGGCTCAAGGTATGCTGGCAATTGTTCATGGTGCTGTGGATGACTTTAAATCAGCGAGTCCTGTCATGAAGGATTCCAAGGCATCCATTAATCTCCAACCTCATATGCTTAACCCTGTTAAAGTTTTTTCTTTGTTCAAAAGAATGCTTGATGAGGATTGTGAACTTCTTAATCTTAAAAGTAGACCAGAGAACCTCATCATGACAACCATTGCTGTGCCACCTATAGCCATTCGTCCTTCCGTTCTTGTGGATGGATCACAGAGTAATGAAAATGACATTACAGAGAGGTTGAAGAAAATTATTCAAACAAACGCTAATGTTCAACTGGACCTATCAGAAGGTTCTACCACAAGATTTTTGTCAAATTGGGACGTACTTCAAGCCGAAGTTGCGCTGTACATAAACAGTGATGTTCGCGGTATTCCTTATCACATCCAACTAAATTCCAAGCCATTGAATGGTTTGGTACAGCGCCTCAAAGGGAAGACGGGCCGTTTTCGTGGAAACCTATGTGGGAAAAGGGTTGAATATACTGGCAGAACTGTTATTTCACCCGACCCTAATCTGAAAATTACTGAGGTCGGAATCCCTATCCAAATGGCTCAGATTTTAAGTTATCCAGAACGTGTGTCCCATCATAATATTGAAAAGTTGAAGCAGTGTGTATTTAATGGACCTTCTAAGTACCCTGGTGCCCTAGTGCTCAAACATCAAAATGGCGAGGAATGGTCCTTGAGGGCTGAGCGACAAAGGAAGTCTCATGCTGATAATCTCCGATATGGTGACATAGTTGATCGCCACTTAGAAGATGGGGACATTGTTCTTTTCAACAGACAGCCAAGCTTGCATCGGATGTCCATCATGTGTCATAGGGCAAGGATTATGCCTTGGAGAACGTTGAGGTTTAATGAATCTGTATGCAACCCTTATAATGCTGATTTTGATGGTGACGAAATGAACATGCATGTGCCGCAAACAGAGGAGGCTCGAACTGAGGCTGTTATGTTGATGGGGGTGCAAAACAATCTATGCACTCCCAAAAATGGGGAGATTTTGGTTGCTTCCACTCAAGATTTTCTAACATCTTCTTTCCTTATAACAAGGAAGGACACATTTTATGACCGTGCATCTTTTTCTCTCATGTGTTCGTACATGGGGGATGGCACGGAGACTGTGGATTTGCCAACACCTGCAGTAATTAAGCCAATAGAGCTATGGACAGGAAAGCAGCTATATAGTGTGTTAGTTCGCCCAAATTCAAATGTGAGAGTATATCTAAACCTAACTGTTAGAGAGAAATCCTACTCCAACACAAAAACTGAAGATGGAAGAGAAACAGAAGCAATGTGCCCAAACGATGGGTTTGTCTATTTCCGTAACAGTGAGCTATTATCTGGGCAACTTGGGAAGGGCACTTTAGGAAATGGCAATAAGGATGGACTTTTCTCTGTTCTTCTCAGAGACTATAAAGCTCATGCTGCTGCTTCTTGCATGAATCGTGTAGCTAAATTGAGTGCCCGGTGGATTGGTAATCACGGATTCTCAATTGGGATTAGTGATGTACAACCTAGTGACATCTTGTACGGTGAGAAAGAAAAAATCATTAAGAAAGGTTATGACAAATGTGACGACCAGATAAAGTTGTATAAAGAAGGACAGCTACGACTTGCACCTGGTTGTGATGCTGCCCAATCACTTGAATCTGGGATTACTGCAGTATTAAACGATATTAGGGACCAAACTGGAAAGCTGTGCATGCAAACGCTACATTGGAGAAACAGTCCCCTGATTATGTCCCAGTGTGGTTCCAAAGGATCTGCTATCAATATCAGCCAGATGGTTGCTTGTGTTGGTCAACAATCAGTTGGTGGTCGTCGTGCACCAAATGGTTTCATAGATCGAAGCCTTCCTCACTTTCCTAGAAAAGAAAATACCCCTGCAGCTAAAGGCTTTGTTGCTAGTTCCTTTTACAGTGGCTTGACAGCTACAGAGTTCTTTTTTCATACAATGGGAGGGCGAGAAGGCCTTGTGGATACAGCTGTAAAAACTGCTGACACAGGGTACATGTCTCGTAGATTGTCTAAAGTTCTAGAGGACCTCTCTGTCCAGTATGATAAAACAGTGCGGGCCGCAAATGGAGGTATTGTTCAGTTTTGTTATGGGGATGACGGTATGGATCCTGCCATGATGGAAGGAAAAAGTGGAGCTCCGTTAGACTTCTACCGATTGTTTTTCAAAGCTAAGGCCACATGCCCTGCTGAAGAAAGTAAAAGCCTTTCTCCTGATGAAGTGTCTGAAATAGTGAGAAGCAGGCTTTCGGAACAAGATATGACTCCTGATGGTGGTTGCTCTATGGGTTTTAAAAGTTCTATAGAGCAATTCCTGGATGAATATGTAAAAGCATTAAGAAAAACACAGGAGGCTTTTTTATTGAATCAAAGTCCTGGTTGGAAGGTGAAGTCTGCAACTGTAGAGAAGATTGTCCAAAATATATCTGGTGTCACTTCTAGACAGCTGAAGGTTTTTCTAGATACTTGTATTTCTCGTTATCATTCAAAAAAAGTTGATGCGGGAACAGCAATTGGAGTAATTGGAGCTCAAAGCATTGGCGAGCCTGGGACACAGATGACATTAAAAACTTTTCACTTTGCTGGAGTTGCAAGCATGAATGTTACACTTGGAGTCCCTCGTATCAAGGAAATAATAAATGGAGCAAAAAATATCCATACCCCTATCATCACTACAGTACTTGAGTGTGATAATAATCCGATGATTGCACGGATGGTAGCAGGTCGGATTGAAAAAACAAATCTAGGGCAGGTTGCTGAGTGTATAGAGATTGTAATGAGTCCAAGAGCAGCATATGTAGCCATCAAGCTTGATATGTTTGTGATACAAGATGCACACTTGTCTATAGATGCCAATGTTGTAAAACAATCAATTTTGGGAACTCCAAGGATTAAACTGAAACATGAGCACATTAGGGTTCCAGATAATCAAACAGTTGAAGTTCATTCTCACGAGAAGGATAGAAGAAAACTCCATTTTCACCTCTTCTATTTGAAGAGTATCCTCCCCAGTGTTATAGTAAGGGGTATAAAGACAATTGAACGTGTTGTCATTGATGAAGAAAAAGTTAAAAGGGACAATAAAGATATTGACGTTAAATGTGGAGAAGAAAAGAAGTACAAGTTATTTGCTGAAGGCAAGGGTCTTCTGGCAGTAATGAGCACTAAAGGAGTAGATGGTTGTAAAACCACAAGTAATGATATTATTGAAGTGCAGCAGACACTTGGAATTGAAGCTGCAAGAACTTGTATCATTGAGGAGATAAAGTATACTATGAAATCACACGGGATGAACATAGATGATCGTCATATGATGCTTTTAGCAGATGTAATGACATTCAGAGGTTTAGTTCTTGGAATCACAAGATTCGGCGTTCAAAAGATGGGCAAAAGTGTATTGATGCTGGCTTCATTTGAGCGAACGGATGATCATCTATTTAATGCTTGCGTTCAGGGGACAGTTGACAAAATTGAAGGAGTAAGTGAAAGCATAATCATGGGTATACCAATGCCAATAGGCACCGGAATGTTCAAAATTAAACAACGCCTCTCTGCAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002376 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0046483 GO:0048518 GO:0050789 GO:0050896 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1481

Amino Acids

165.38

Weight (kDa)

8.85

Isoelectric Point (pI)

39.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	116 - 442	5.2e-75	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	444 - 611	5.4e-74	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	614 - 793	7.2e-38	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	821 - 924	4.3e-29	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	931 - 1426	2e-88	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60040 AT5G60040
fragaria_vesca	FvH4_3g34290 FvH4_3g34290 FvH4_3g34290 FvH4_6g15631
malus_domestica	MD03G1106800.v1.1 MD09G1157000.v1.1
prunus_persica	Prupe.6G089600_v2.0.a1 Prupe.6G089600_v2.0.a1
pyrus_communis	pycom03g08620 pycom09g07570
rosa_chinensis	RchiOBHm_Chr3g0469301 RchiOBHm_Chr3g0469321 RchiOBHm_Chr5g0061861
rosa_laevigata	RLG00000024360 RLG00000035511
rosa_multiflora	Rmu_sc0004134.1_g000001 Rmu_sc0011641.1_g000013 Rmu_sc0011641.1_g000015 Rmu_sc0019271.1_g000001 Rmu_sc0022191.1_g000001
rosa_roxburghii	Rroxscaffold_1G00018870 Rroxscaffold_6G00411990 Rroxscaffold_6G00412000
rosa_rugosa	Rorug03G0100400 Rorug05G0344900 Rorug05G0345000
rosa_samantha	Rh3AG149800 Rh3BG173400 Rh3CG164500 Rh3DG202000 Rh5AG405500 Rh5BG418300 Rh5CG442800 Rh5DG432400
rosa_wichuraiana	Rw5G038070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1746, 1913
AarI	CACCTGC	1 cut(s)	2014
AasI	GACNNNNNNGTC	1 cut(s)	2918
Acc36I	ACCTGC	3 cut(s)	2014, 3596, 3625
AccB1I	GGYRCC	2 cut(s)	1508, 4403
AccB7I	CCANNNNNTGG	2 cut(s)	1274, 2174
AccI	GTMKAC	3 cut(s)	997, 4154, 4387
AccII	CGCG	1 cut(s)	1233
AciI	CCGC	7 cut(s)	475, 501, 1233, 1787, 2941, 2946, 3386
AclI	AACGTT	1 cut(s)	1712
AclWI	GGATC	7 cut(s)	464, 477, 689, 1078, 2638, 2987, 3000
AcoI	YGGCCR	1 cut(s)	544
AcsI	RAATTY	4 cut(s)	17, 411, 1258, 2071
AcuI	CTGAAG	4 cut(s)	2157, 3096, 3353, 4038
AfiI	CCNNNNNNNGG	8 cut(s)	455, 479, 788, 1274, 1859, 1860, 2174, 3158
AflIII	ACRYGT	4 cut(s)	515, 1441, 2880, 3928
AjnI	CCWGG	6 cut(s)	541, 1504, 2488, 3198, 3259, 3430
AloI	GAACNNNNNNTCC	2 cut(s)	2946, 2978
Alw21I	GWGCWC	6 cut(s)	1521, 2698, 3025, 3415, 3795, 4049
Alw26I	GTCTC	6 cut(s)	250, 506, 1578, 1979, 2271, 2889
Alw44I	GTGCAC	1 cut(s)	2694
AlwI	GGATC	7 cut(s)	464, 477, 689, 1078, 2638, 2987, 3000
AoxI	GGCC	7 cut(s)	201, 481, 544, 1306, 2839, 2943, 3060
ApaI	GGGCCC	1 cut(s)	485
ApaLI	GTGCAC	1 cut(s)	2694
ApeKI	GCWGC	9 cut(s)	401, 2044, 2294, 2297, 2501, 2756, 3674, 4099, 4121
ApoI	RAATTY	4 cut(s)	17, 411, 1258, 2071
ArsI	GACNNNNNNTTYG	2 cut(s)	1978, 2010
AseI	ATTAAT	1 cut(s)	903
Asp700I	GAANNNNTTC	1 cut(s)	17
AspLEI	GCGC	2 cut(s)	1210, 1290
AsuC2I	CCSGG	1 cut(s)	2335
AsuHPI	GGTGA	7 cut(s)	515, 1359, 1610, 1775, 2414, 3283, 3857
AvaII	GGWCC	5 cut(s)	1144, 1490, 1545, 2551, 2911
BaeGI	GKGCMC	8 cut(s)	342, 485, 782, 1513, 2168, 2231, 2335, 2698
BaeI	ACNNNNGTAYC	2 cut(s)	2841, 2874
BalI	TGGCCA	1 cut(s)	546
BamHI	GGATCC	2 cut(s)	469, 2992
BanI	GGYRCC	2 cut(s)	1508, 4403
BanII	GRGCYC	4 cut(s)	485, 820, 3025, 3415
BarI	GAAGNNNNNNTAC	2 cut(s)	2460, 2492
BauI	CACGAG	1 cut(s)	3836
BbsI	GAAGAC	2 cut(s)	1307, 4022
Bbv12I	GWGCWC	6 cut(s)	1521, 2698, 3025, 3415, 3795, 4049
BbvI	GCAGC	9 cut(s)	388, 2056, 2281, 2284, 2488, 2768, 3686, 4108, 4111
BceAI	ACGGC	1 cut(s)	1293
BciT130I	CCWGG	6 cut(s)	543, 1506, 2490, 3200, 3261, 3432
BciVI	GTATCC	2 cut(s)	2842, 3896
BclI	TGATCA	1 cut(s)	4312
BcnI	CCSGG	1 cut(s)	2335
BcoDI	GTCTC	6 cut(s)	250, 506, 1578, 1979, 2271, 2889
BfaI	CTAG	9 cut(s)	747, 1514, 2385, 2736, 2775, 2906, 3324, 3344, 3629
BfoI	RGCGCY	1 cut(s)	1291
BfuAI	ACCTGC	3 cut(s)	2014, 3596, 3625
BfuI	GTATCC	2 cut(s)	2842, 3896
BlpI	GCTNAGC	1 cut(s)	1557
BmcAI	AGTACT	2 cut(s)	601, 3564
Bme1390I	CCNGG	7 cut(s)	543, 1506, 2335, 2490, 3200, 3261, 3432
Bme18I	GGWCC	5 cut(s)	1144, 1490, 1545, 2551, 2911
BmiI	GGNNCC	9 cut(s)	204, 471, 483, 1510, 2552, 2623, 2994, 3804, 4405
BmrFI	CCNGG	7 cut(s)	543, 1506, 2335, 2490, 3200, 3261, 3432
BmrI	ACTGGG	3 cut(s)	373, 2608, 3888
BmsI	GCATC	9 cut(s)	905, 1672, 1952, 2488, 3373, 3706, 3724, 4188, 4275
BmuI	ACTGGG	3 cut(s)	373, 2608, 3888
BpiI	GAAGAC	2 cut(s)	1307, 4022
BplI	GAGNNNNNCTC	2 cut(s)	2900, 2932
BpmI	CTGGAG	1 cut(s)	3489
Bpu10I	CCTNAGC	1 cut(s)	3057
Bpu1102I	GCTNAGC	1 cut(s)	1557
BpuEI	CTTGAG	4 cut(s)	804, 1570, 1869, 3587
BpuMI	CCSGG	1 cut(s)	2335
BsaBI	GATNNNNATC	4 cut(s)	438, 1673, 3579, 4314
BsaJI	CCNNGG	5 cut(s)	891, 1504, 1703, 3431, 3770
BsaWI	WCCGGW	2 cut(s)	265, 4406
BsaXI	ACNNNNNCTCC	6 cut(s)	2581, 2611, 2946, 2976, 4136, 4166
Bsc4I	CCNNNNNNNGG	8 cut(s)	455, 479, 788, 1274, 1859, 1860, 2174, 3158
Bse1I	ACTGG	7 cut(s)	379, 1146, 1354, 2563, 2614, 2923, 3894
Bse3DI	GCAATG	3 cut(s)	503, 1023, 2166
Bse8I	GATNNNNATC	4 cut(s)	438, 1673, 3579, 4314
BseBI	CCWGG	6 cut(s)	543, 1506, 2490, 3200, 3261, 3432
BseDI	CCNNGG	5 cut(s)	891, 1504, 1703, 3431, 3770
BseJI	GATNNNNATC	4 cut(s)	438, 1673, 3579, 4314
BseLI	CCNNNNNNNGG	8 cut(s)	455, 479, 788, 1274, 1859, 1860, 2174, 3158
BseMI	GCAATG	3 cut(s)	503, 1023, 2166
BseMII	CTCAG	6 cut(s)	1383, 1433, 1548, 1800, 2287, 3633
BseNI	ACTGG	7 cut(s)	379, 1146, 1354, 2563, 2614, 2923, 3894
BseRI	GAGGAG	3 cut(s)	143, 1811, 4157
BseSI	GKGCMC	8 cut(s)	342, 485, 782, 1513, 2168, 2231, 2335, 2698
BseXI	GCAGC	9 cut(s)	388, 2056, 2281, 2284, 2488, 2768, 3686, 4108, 4111
BsgI	GTGCAG	1 cut(s)	4118
Bsh1236I	CGCG	1 cut(s)	1233
BshFI	GGCC	7 cut(s)	203, 483, 546, 1308, 2841, 2945, 3062
BshNI	GGYRCC	2 cut(s)	1508, 4403
BsiHKAI	GWGCWC	6 cut(s)	1521, 2698, 3025, 3415, 3795, 4049
BsiSI	CCGG	3 cut(s)	266, 2335, 4407
BslI	CCNNNNNNNGG	8 cut(s)	455, 479, 788, 1274, 1859, 1860, 2174, 3158
BsmAI	GTCTC	6 cut(s)	250, 506, 1578, 1979, 2271, 2889
BsmI	GAATGC	2 cut(s)	669, 963
BsnI	GGCC	7 cut(s)	203, 483, 546, 1308, 2841, 2945, 3062
Bsp120I	GGGCCC	1 cut(s)	481
Bsp1407I	TGTACA	2 cut(s)	1212, 2377
Bsp1720I	GCTNAGC	1 cut(s)	1557
BspACI	CCGC	7 cut(s)	475, 501, 1233, 1787, 2941, 2946, 3386
BspANI	GGCC	7 cut(s)	203, 483, 546, 1308, 2841, 2945, 3062
BspCNI	CTCAG	6 cut(s)	1384, 1432, 1549, 1801, 2286, 3634
BspFNI	CGCG	1 cut(s)	1233
BspHI	TCATGA	2 cut(s)	879, 1014
BspLI	GGNNCC	9 cut(s)	204, 471, 483, 1510, 2552, 2623, 2994, 3804, 4405
BspMAI	CTGCAG	4 cut(s)	347, 2011, 2533, 2758
BspMI	ACCTGC	3 cut(s)	2014, 3596, 3625
BspPI	GGATC	7 cut(s)	464, 477, 689, 1078, 2638, 2987, 3000
BspQI	GCTCTTC	1 cut(s)	42
BspT107I	GGYRCC	2 cut(s)	1508, 4403
BsrDI	GCAATG	3 cut(s)	503, 1023, 2166
BsrGI	TGTACA	2 cut(s)	1212, 2377
BsrI	ACTGG	7 cut(s)	379, 1146, 1354, 2563, 2614, 2923, 3894
BssECI	CCNNGG	5 cut(s)	891, 1504, 1703, 3431, 3770
BssNAI	GTATAC	2 cut(s)	4155, 4388
BssSI	CACGAG	1 cut(s)	3836
BssT1I	CCWWGG	3 cut(s)	891, 1703, 3770
Bst1107I	GTATAC	2 cut(s)	4155, 4388
Bst2BI	CACGAG	1 cut(s)	3836
Bst2UI	CCWGG	6 cut(s)	543, 1506, 2490, 3200, 3261, 3432
Bst6I	CTCTTC	6 cut(s)	42, 248, 806, 2140, 3875, 3875
BstAUI	TGTACA	2 cut(s)	1212, 2377
BstDEI	CTNAG	9 cut(s)	1392, 1419, 1497, 1557, 1618, 1809, 2273, 3057, 3642
BstFNI	CGCG	1 cut(s)	1233
BstH2I	RGCGCY	1 cut(s)	1291
BstHHI	GCGC	2 cut(s)	1210, 1290
BstMAI	GTCTC	6 cut(s)	250, 506, 1578, 1979, 2271, 2889
BstNI	CCWGG	6 cut(s)	543, 1506, 2490, 3200, 3261, 3432
BstNSI	RCATGY	6 cut(s)	519, 1780, 1784, 2575, 2884, 3069
BstSCI	CCNGG	7 cut(s)	541, 1504, 2333, 2488, 3198, 3259, 3430
BstSLI	GKGCMC	8 cut(s)	342, 485, 782, 1513, 2168, 2231, 2335, 2698
BstUI	CGCG	1 cut(s)	1233
BstV1I	GCAGC	9 cut(s)	388, 2056, 2281, 2284, 2488, 2768, 3686, 4108, 4111
BstV2I	GAAGAC	2 cut(s)	1307, 4022
BstX2I	RGATCY	4 cut(s)	469, 694, 2630, 2992
BstYI	RGATCY	4 cut(s)	469, 694, 2630, 2992
BstZ17I	GTATAC	2 cut(s)	4155, 4388
BsuI	GTATCC	2 cut(s)	2842, 3896
BsuRI	GGCC	7 cut(s)	203, 483, 546, 1308, 2841, 2945, 3062
BtsI	GCAGTG	2 cut(s)	340, 1481
BveI	ACCTGC	3 cut(s)	2014, 3596, 3625
CciI	TCATGA	2 cut(s)	879, 1014
CfoI	GCGC	2 cut(s)	1210, 1290
CsiI	ACCWGGT	1 cut(s)	2488
DdeI	CTNAG	9 cut(s)	1392, 1419, 1497, 1557, 1618, 1809, 2273, 3057, 3642
DraI	TTTAAA	2 cut(s)	862, 3178
DrdI	GACNNNNNNGTC	1 cut(s)	2918
DseDI	GACNNNNNNGTC	1 cut(s)	2918
EaeI	YGGCCR	1 cut(s)	544
Eam1104I	CTCTTC	6 cut(s)	42, 248, 806, 2140, 3875, 3875
EarI	CTCTTC	6 cut(s)	42, 248, 806, 2140, 3875, 3875
Ecl136II	GAGCTC	2 cut(s)	3023, 3413
Eco130I	CCWWGG	3 cut(s)	891, 1703, 3770
Eco147I	AGGCCT	1 cut(s)	2841
Eco24I	GRGCYC	4 cut(s)	485, 820, 3025, 3415
Eco47I	GGWCC	5 cut(s)	1144, 1490, 1545, 2551, 2911
Eco53kI	GAGCTC	2 cut(s)	3023, 3413
Eco57I	CTGAAG	4 cut(s)	2157, 3096, 3353, 4038
EcoICRI	GAGCTC	2 cut(s)	3023, 3413
EcoO109I	RGGNCCY	3 cut(s)	202, 482, 2911
EcoRII	CCWGG	6 cut(s)	541, 1504, 2488, 3198, 3259, 3430
EcoT14I	CCWWGG	3 cut(s)	891, 1703, 3770
EcoT22I	ATGCAT	1 cut(s)	1782
EcoT38I	GRGCYC	4 cut(s)	485, 820, 3025, 3415
ErhI	CCWWGG	3 cut(s)	891, 1703, 3770
FauI	CCCGC	3 cut(s)	482, 2934, 3379
FauNDI	CATATG	3 cut(s)	918, 3679, 4194
FbaI	TGATCA	1 cut(s)	4312
FblI	GTMKAC	3 cut(s)	997, 4154, 4387
FriOI	GRGCYC	4 cut(s)	485, 820, 3025, 3415
FspBI	CTAG	9 cut(s)	747, 1514, 2385, 2736, 2775, 2906, 3324, 3344, 3629
GlaI	GCGC	2 cut(s)	1209, 1289
GsuI	CTGGAG	1 cut(s)	3489
HaeII	RGCGCY	1 cut(s)	1291
HaeIII	GGCC	7 cut(s)	203, 483, 546, 1308, 2841, 2945, 3062
HapII	CCGG	3 cut(s)	266, 2335, 4407
HhaI	GCGC	2 cut(s)	1210, 1290
Hin6I	GCGC	2 cut(s)	1208, 1288
HinP1I	GCGC	2 cut(s)	1208, 1288
HincII	GTYRAC	2 cut(s)	2672, 4348
HindII	GTYRAC	2 cut(s)	2672, 4348
HindIII	AAGCTT	2 cut(s)	1657, 3692
HpaII	CCGG	3 cut(s)	266, 2335, 4407
HphI	GGTGA	7 cut(s)	515, 1359, 1610, 1775, 2414, 3283, 3857
Hpy166II	GTNNAC	9 cut(s)	610, 744, 977, 998, 2672, 2696, 4155, 4348, 4388
Hpy8I	GTNNAC	9 cut(s)	610, 744, 977, 998, 2672, 2696, 4155, 4348, 4388
Hpy99I	CGWCG	1 cut(s)	2694
HpyCH4IV	ACGT	6 cut(s)	612, 1188, 1441, 1712, 3928, 3978
HpyF3I	CTNAG	9 cut(s)	1392, 1419, 1497, 1557, 1618, 1809, 2273, 3057, 3642
HpySE526I	ACGT	6 cut(s)	612, 1188, 1441, 1712, 3928, 3978
HspAI	GCGC	2 cut(s)	1208, 1288
Ksp22I	TGATCA	1 cut(s)	4312
LguI	GCTCTTC	1 cut(s)	42
LmnI	GCTCC	5 cut(s)	130, 3020, 3028, 3410, 3527
Lsp1109I	GCAGC	9 cut(s)	388, 2056, 2281, 2284, 2488, 2768, 3686, 4108, 4111
LweI	GCATC	9 cut(s)	905, 1672, 1952, 2488, 3373, 3706, 3724, 4188, 4275
MabI	ACCWGGT	1 cut(s)	2488
MaeI	CTAG	9 cut(s)	747, 1514, 2385, 2736, 2775, 2906, 3324, 3344, 3629
MaeII	ACGT	6 cut(s)	612, 1188, 1441, 1712, 3928, 3978
MfeI	CAATTG	4 cut(s)	834, 2361, 3396, 3921
MflI	RGATCY	4 cut(s)	469, 694, 2630, 2992
MlsI	TGGCCA	1 cut(s)	546
MluNI	TGGCCA	1 cut(s)	546
MlyI	GAGTC	5 cut(s)	65, 880, 3142, 3507, 3673
MmeI	TCCRAC	8 cut(s)	447, 568, 934, 1276, 1378, 2148, 3245, 3590
Mox20I	TGGCCA	1 cut(s)	546
Mph1103I	ATGCAT	1 cut(s)	1782
MroXI	GAANNNNTTC	1 cut(s)	17
MscI	TGGCCA	1 cut(s)	546
MslI	CAYNNNNRTG	6 cut(s)	84, 1028, 1530, 1781, 4379, 4409
Msp20I	TGGCCA	1 cut(s)	546
MspA1I	CMGCKG	2 cut(s)	2855, 3331
MspI	CCGG	3 cut(s)	266, 2335, 4407
MspR9I	CCNGG	7 cut(s)	543, 1506, 2335, 2490, 3200, 3261, 3432
MunI	CAATTG	4 cut(s)	834, 2361, 3396, 3921
Mva1269I	GAATGC	2 cut(s)	669, 963
MvaI	CCWGG	6 cut(s)	543, 1506, 2490, 3200, 3261, 3432
MvnI	CGCG	1 cut(s)	1233
NciI	CCSGG	1 cut(s)	2335
NdeI	CATATG	3 cut(s)	918, 3679, 4194
NlaIV	GGNNCC	9 cut(s)	204, 471, 483, 1510, 2552, 2623, 2994, 3804, 4405
NmuCI	GTSAC	7 cut(s)	503, 715, 1598, 1763, 2387, 2441, 3316
NsiI	ATGCAT	1 cut(s)	1782
NspI	RCATGY	6 cut(s)	519, 1780, 1784, 2575, 2884, 3069
PaeI	GCATGC	1 cut(s)	2575
PagI	TCATGA	2 cut(s)	879, 1014
PaqCI	CACCTGC	1 cut(s)	2014
PceI	AGGCCT	1 cut(s)	2841
PciI	ACATGT	2 cut(s)	515, 2880
PciSI	GCTCTTC	1 cut(s)	42
PctI	GAATGC	2 cut(s)	669, 963
PdmI	GAANNNNTTC	1 cut(s)	17
PfeI	GAWTC	9 cut(s)	887, 1401, 1727, 2311, 2355, 2516, 3250, 4242, 4251
PflMI	CCANNNNNTGG	2 cut(s)	1274, 2174
PfoI	TCCNGGA	1 cut(s)	3198
PleI	GAGTC	5 cut(s)	64, 879, 3142, 3506, 3672
PpsI	GAGTC	5 cut(s)	64, 879, 3142, 3506, 3672
PpuMI	RGGWCCY	1 cut(s)	2911
PscI	ACATGT	2 cut(s)	515, 2880
PshBI	ATTAAT	1 cut(s)	903
PsiI	TTATAA	2 cut(s)	1746, 1913
Psp124BI	GAGCTC	2 cut(s)	3025, 3415
Psp1406I	AACGTT	1 cut(s)	1712
Psp5II	RGGWCCY	1 cut(s)	2911
Psp6I	CCWGG	6 cut(s)	541, 1504, 2488, 3198, 3259, 3430
PspGI	CCWGG	6 cut(s)	541, 1504, 2488, 3198, 3259, 3430
PspN4I	GGNNCC	9 cut(s)	204, 471, 483, 1510, 2552, 2623, 2994, 3804, 4405
PspOMI	GGGCCC	1 cut(s)	481
PspPPI	RGGWCCY	1 cut(s)	2911
PstI	CTGCAG	4 cut(s)	347, 2011, 2533, 2758
PsuI	RGATCY	4 cut(s)	469, 694, 2630, 2992
PvuII	CAGCTG	2 cut(s)	2855, 3331
RseI	CAYNNNNRTG	6 cut(s)	84, 1028, 1530, 1781, 4379, 4409
SacI	GAGCTC	2 cut(s)	3025, 3415
SapI	GCTCTTC	1 cut(s)	42
ScaI	AGTACT	2 cut(s)	601, 3564
SchI	GAGTC	5 cut(s)	65, 880, 3142, 3507, 3673
ScrFI	CCNGG	7 cut(s)	543, 1506, 2335, 2490, 3200, 3261, 3432
SexAI	ACCWGGT	1 cut(s)	2488
SfaNI	GCATC	9 cut(s)	905, 1672, 1952, 2488, 3373, 3706, 3724, 4188, 4275
SinI	GGWCC	5 cut(s)	1144, 1490, 1545, 2551, 2911
SmiMI	CAYNNNNRTG	6 cut(s)	84, 1028, 1530, 1781, 4379, 4409
SmlI	CTYRAG	4 cut(s)	819, 1549, 1884, 3566
SmoI	CTYRAG	4 cut(s)	819, 1549, 1884, 3566
SphI	GCATGC	1 cut(s)	2575
SseBI	AGGCCT	1 cut(s)	2841
SsiI	CCGC	7 cut(s)	475, 501, 1233, 1787, 2941, 2946, 3386
SspI	AATATT	2 cut(s)	685, 1459
SspMI	CTAG	9 cut(s)	747, 1514, 2385, 2736, 2775, 2906, 3324, 3344, 3629
SstI	GAGCTC	2 cut(s)	3025, 3415
StuI	AGGCCT	1 cut(s)	2841
StyD4I	CCNGG	7 cut(s)	541, 1504, 2333, 2488, 3198, 3259, 3430
StyI	CCWWGG	3 cut(s)	891, 1703, 3770
TaiI	ACGT	6 cut(s)	615, 1191, 1444, 1715, 3931, 3981
TaqI	TCGA	5 cut(s)	82, 424, 643, 1804, 2716
TatI	WGTACW	6 cut(s)	599, 743, 1212, 2377, 3562, 4002
TauI	GCSGC	2 cut(s)	1789, 2948
TfiI	GAWTC	9 cut(s)	887, 1401, 1727, 2311, 2355, 2516, 3250, 4242, 4251
TseFI	GTSAC	7 cut(s)	503, 715, 1598, 1763, 2387, 2441, 3316
TseI	GCWGC	9 cut(s)	401, 2044, 2294, 2297, 2501, 2756, 3674, 4099, 4121
Tsp45I	GTSAC	7 cut(s)	503, 715, 1598, 1763, 2387, 2441, 3316
TspGWI	ACGGA	7 cut(s)	1045, 1997, 2180, 2367, 3015, 3610, 4322
Van91I	CCANNNNNTGG	2 cut(s)	1274, 2174
VneI	GTGCAC	1 cut(s)	2694
VpaK11BI	GGWCC	5 cut(s)	1144, 1490, 1545, 2551, 2911
VspI	ATTAAT	1 cut(s)	903
XapI	RAATTY	4 cut(s)	17, 411, 1258, 2071
XbaI	TCTAGA	3 cut(s)	2905, 3323, 3343
XceI	RCATGY	6 cut(s)	519, 1780, 1784, 2575, 2884, 3069
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1622, 2028, 3308
XmiI	GTMKAC	3 cut(s)	997, 4154, 4387
XmnI	GAANNNNTTC	1 cut(s)	17
XspI	CTAG	9 cut(s)	747, 1514, 2385, 2736, 2775, 2906, 3324, 3344, 3629
ZrmI	AGTACT	2 cut(s)	601, 3564
Zsp2I	ATGCAT	1 cut(s)	1782

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.