Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0003176

Physical Location & Seq

Genomic Coordinates Reverse (-)

61484322 .. 61509116

Gene Structure 24795 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG442800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4470 bp

ATGAAAGAGCTGAATAAATTCAAGATAACCTATGAATATGATGATGAAGAGCCAGAGTCCTCCTACCAAATCATCTATCATCGAGATGATTTGGAGAAGTTACAAAAATTGGGGGATGAGAAGAAGGAGGAGCATAAGCAAGGAGAAAGAAAGGGGCAAAAGGAAGGAGAAAGAAAGGATAACAAGGAAGGAGATAGAAAGGCCCCTTCAACCTTCAACCTTGAACCCTCCCTCTTTGCTTTCTGCGTGGAGAAGAGACTTCTACCGGATTTTCTCTCTTCAATTTTGATAGAAAAGATGATGAATAAAGTCCAAGCCAAAACATTAGCCTTCACAAAAGAGCCTTTCATTGAAGAAGAAGGGCACTGCAGAATAGCACAATGGCAATTCAATGCTCTGTCCCAGTCAGAGATCAGCAAAATGGCTGCAACTCAAATTTTTCTGGGTCGATATTACGATAGCAATCAAAAACCAATCAAAGGAGGCTTGTTGGATCCCCGCTTGGGCCCTGCAAATAGGCAATGCGGTGACTGTGAGACATGTGGTGCGAAACTTTCAGATTGTCCTGGCCACTTTGGATATTTGGTGCTTGCTGTCCCTGTATTCAATGTTGGATATTTAAGTACTGTTGTGGACGTTTTGAAGTGCATTTGTAAGTCATGCTCTCGAATAATTTTGGATGACAAATCACGCATTCGCTTTTTGAATATTGTGAGAGATCCCAAAAAGGATAAAAGCGTGACAATGAAATCAATAGTGAAGAAGTGTACTAGCATGGCAAGCAATAATAGATCAGTGAAGTGCCCGAAATGTGGATATATGAATGGTTCAGTGAAGAGGGCTCAAGGTATGCTGGCAATTGTTCATGGTGCTGTGGATGACTTTAAATCAGCGAGTCCTGTCATGAAGGATTCCAAGGCATCCATTAATCTCCAACCTCATATGCTTAACCCTGTTAAAGTTTTTTCTTTGTTCAAAAGAATGCTTGATGAGGATTGTGAACTTCTTAATCTTAAAAGTAGACCAGAGAACCTCATCATGACAACCATTGCTGTGCCACCTATAGCCATTCGTCCTTCCGTTCTTGTGGATGGATCACAGAGTAATGAAAATGACATTACAGAGAGGTTGAAGAAAATTATTCAAACAAACGCTAATGTTCAACTGGACCTATCAGAAGGTTCTACCACAAGATTTTTGTCAAATTGGGACGTACTTCAAGCCGAAGTTGCGCTGTACATAAACAGTGATGTTCGCGGTATTCCTTATCACATCCAACTAAATTCCAAGCCATTGAATGGTTTGGTACAGCGCCTCAAAGGGAAGACGGGCCGTTTTCGTGGAAACCTATGTGGGAAAAGGGTTGAATATACTGGCAGAACTGTTATTTCACCCGACCCTAATCTGAAAATTACTGAGGTCGGAATCCCTATCCAAATGGCTCAGATTTTAAGTTATCCAGAACGTGTGTCCCATCATAATATTGAAAAGTTGAAGCAGTGTGTATTTAATGGACCTTCTAAGTACCCTGGTGCCCTAGTGCTCAAACATCAAAATGGCGAGGAATGGTCCTTGAGGGCTGAGCGACAAAGGAAGTCTCATGCTGATAATCTCCGATATGGTGACATAGTTGATCGCCACTTAGAAGATGGGGACATTGTTCTTTTCAACAGACAGCCAAGCTTGCATCGGATGTCCATCATGTGTCATAGGGCAAGGATTATGCCTTGGAGAACGTTGAGGTTTAATGAATCTGTATGCAACCCTTATAATGCTGATTTTGATGGTGACGAAATGAACATGCATGTGCCGCAAACAGAGGAGGCTCGAACTGAGGCTGTTATGTTGATGGGGGTGCAAAACAATCTATGCACTCCCAAAAATGGGGAGATTTTGGTTGCTTCCACTCAAGATTTTCTAACATCTTCTTTCCTTATAACAAGGAAGGACACATTTTATGACCGTGCATCTTTTTCTCTCATGTGTTCGTACATGGGGGATGGCACGGAGACTGTGGATTTGCCAACACCTGCAGTAATTAAGCCAATAGAGCTATGGACAGGAAAGCAGCTATATAGTGTGTTAGTTCGCCCAAATTCAAATGTGAGAGTATATCTAAACCTAACTGTTAGAGAGAAATCCTACTCCAACACAAAAACTGAAGATGGAAGAGAAACAGAAGCAATGTGCCCAAACGATGGGTTTGTCTATTTCCGTAACAGTGAGCTATTATCTGGGCAACTTGGGAAGGGCACTTTAGGAAATGGCAATAAGGATGGACTTTTCTCTGTTCTTCTCAGAGACTATAAAGCTCATGCTGCTGCTTCTTGCATGAATCGTGTAGCTAAATTGAGTGCCCGGTGGATTGGTAATCACGGATTCTCAATTGGGATTAGTGATGTACAACCTAGTGACATCTTGTACGGTGAGAAAGAAAAAATCATTAAGAAAGGTTATGACAAATGTGACGACCAGATAAAGTTGTATAAAGAAGGACAGCTACGACTTGCACCTGGTTGTGATGCTGCCCAATCACTTGAATCTGGGATTACTGCAGTATTAAACGATATTAGGGACCAAACTGGAAAGCTGTGCATGCAAACGCTACATTGGAGAAACAGTCCCCTGATTATGTCCCAGTGTGGTTCCAAAGGATCTGCTATCAATATCAGCCAGATGGTTGCTTGTGTTGGTCAACAATCAGTTGGTGGTCGTCGTGCACCAAATGGTTTCATAGATCGAAGCCTTCCTCACTTTCCTAGAAAAGAAAATACCCCTGCAGCTAAAGGCTTTGTTGCTAGTTCCTTTTACAGTGGCTTGACAGCTACAGAGTTCTTTTTTCATACAATGGGAGGGCGAGAAGGCCTTGTGGATACAGCTGTAAAAACTGCTGACACAGGGTACATGTCTCGTAGATTGTCTAAAGTTCTAGAGGACCTCTCTGTCCAGTATGATAAAACAGTGCGGGCCGCAAATGGAGGTATTGTTCAGTTTTGTTATGGGGATGACGGTATGGATCCTGCCATGATGGAAGGAAAAAGTGGAGCTCCGTTAGACTTCTACCGATTGTTTTTCAAAGCTAAGGCCACATGCCCTGCTGAAGAAAGTAAAAGCCTTTCTCCTGATGAAGTGTCTGAAATAGTGAGAAGCAGGCTTTCGGAACAAGATATGACTCCTGATGGTGGTTGCTCTATGGGTTTTAAAAGTTCTATAGAGCAATTCCTGGATGAATATGTAAAAGCATTAAGAAAAACACAGGAGGCTTTTTTATTGAATCAAAGTCCTGGTTGGAAGGTGAAGTCTGCAACTGTAGAGAAGATTGTCCAAAATATATCTGGTGTCACTTCTAGACAGCTGAAGGTTTTTCTAGATACTTGTATTTCTCGTTATCATTCAAAAAAAGTTGATGCGGGAACAGCAATTGGAGTAATTGGAGCTCAAAGCATTGGCGAGCCTGGGACACAGATGACATTAAAAACTTTTCACTTTGCTGGAGTTGCAAGCATGAATGTTACACTTGGAGTCCCTCGTATCAAGGAAATAATAAATGGAGCAAAAAATATCCATACCCCTATCATCACTACAGTACTTGAGTGTGATAATAATCCGATGATTGCACGGATGGTAGCAGGTCGGATTGAAAAAACAAATCTAGGGCAGGTTGCTGAGTGTATAGAGATTGTAATGAGTCCAAGAGCAGCATATGTAGCCATCAAGCTTGATATGTTTGTGATACAAGATGCACACTTGTCTATAGATGCCAATGTTGTAAAACAATCAATTTTGGGAACTCCAAGGATTAAACTGAAACATGAGCACATTAGGGTTCCAGATAATCAAACAGTTGAAGTTCATTCTCACGAGAAGGATAGAAGAAAACTCCATTTTCACCTCTTCTATTTGAAGAGTATCCTCCCCAGTGTTATAGTAAGGGGTATAAAGACAATTGAACGTGTTGTCATTGATGAAGAAAAAGTTAAAAGGGACAATAAAGATATTGACGTTAAATGTGGAGAAGAAAAGAAGTACAAGTTATTTGCTGAAGGCAAGGGTCTTCTGGCAGTAATGAGCACTAAAGGAGTAGATGGTTGTAAAACCACAAGTAATGATATTATTGAAGTGCAGCAGACACTTGGAATTGAAGCTGCAAGAACTTGTATCATTGAGGAGATAAAGTATACTATGAAATCACACGGGATGAACATAGATGATCGTCATATGATGCTTTTAGCAGATGTAATGACATTCAGAGGTTTAGTTCTTGGAATCACAAGATTCGGCGTTCAAAAGATGGGCAAAAGTGTATTGATGCTGGCTTCATTTGAGCGAACGGATGATCATCTATTTAATGCTTGCGTTCAGGGGACAGTTGACAAAATTGAAGGAGTAAGTGAAAGCATAATCATGGGTATACCAATGCCAATAGGCACCGGAATGTTCAAAATTAAACAACGCCTCTCTGCAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002376 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0046483 GO:0048518 GO:0050789 GO:0050896 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1489

Amino Acids

166.27

Weight (kDa)

8.82

Isoelectric Point (pI)

39.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	124 - 450	5.3e-75	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	452 - 619	5.5e-74	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	622 - 801	7.2e-38	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	829 - 932	4.4e-29	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	939 - 1434	2e-88	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60040 AT5G60040
fragaria_vesca	FvH4_3g34290 FvH4_3g34290 FvH4_3g34290 FvH4_6g15631
malus_domestica	MD03G1106800.v1.1 MD09G1157000.v1.1
prunus_persica	Prupe.6G089600_v2.0.a1 Prupe.6G089600_v2.0.a1
pyrus_communis	pycom03g08620 pycom09g07570
rosa_chinensis	RchiOBHm_Chr3g0469301 RchiOBHm_Chr3g0469321 RchiOBHm_Chr5g0061861
rosa_laevigata	RLG00000024360 RLG00000035511
rosa_multiflora	Rmu_sc0004134.1_g000001 Rmu_sc0011641.1_g000013 Rmu_sc0011641.1_g000015 Rmu_sc0019271.1_g000001 Rmu_sc0022191.1_g000001
rosa_roxburghii	Rroxscaffold_1G00018870 Rroxscaffold_6G00411990 Rroxscaffold_6G00412000
rosa_rugosa	Rorug03G0100400 Rorug05G0344900 Rorug05G0345000
rosa_samantha	Rh3AG149800 Rh3BG173400 Rh3CG164500 Rh3DG202000 Rh5AG405500 Rh5BG418300 Rh5CG442800 Rh5DG432400
rosa_wichuraiana	Rw5G038070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1770, 1937
AarI	CACCTGC	1 cut(s)	2038
AasI	GACNNNNNNGTC	1 cut(s)	2942
Acc36I	ACCTGC	3 cut(s)	2038, 3620, 3649
AccB1I	GGYRCC	2 cut(s)	1532, 4427
AccB7I	CCANNNNNTGG	2 cut(s)	1298, 2198
AccI	GTMKAC	3 cut(s)	1021, 4178, 4411
AccII	CGCG	1 cut(s)	1257
AciI	CCGC	7 cut(s)	499, 525, 1257, 1811, 2965, 2970, 3410
AclI	AACGTT	1 cut(s)	1736
AclWI	GGATC	7 cut(s)	488, 501, 713, 1102, 2662, 3011, 3024
AcoI	YGGCCR	1 cut(s)	568
AcsI	RAATTY	4 cut(s)	17, 435, 1282, 2095
AcuI	CTGAAG	4 cut(s)	2181, 3120, 3377, 4062
AfiI	CCNNNNNNNGG	8 cut(s)	479, 503, 812, 1298, 1883, 1884, 2198, 3182
AflIII	ACRYGT	4 cut(s)	539, 1465, 2904, 3952
AjnI	CCWGG	6 cut(s)	565, 1528, 2512, 3222, 3283, 3454
AloI	GAACNNNNNNTCC	2 cut(s)	2970, 3002
Alw21I	GWGCWC	6 cut(s)	1545, 2722, 3049, 3439, 3819, 4073
Alw26I	GTCTC	6 cut(s)	250, 530, 1602, 2003, 2295, 2913
Alw44I	GTGCAC	1 cut(s)	2718
AlwI	GGATC	7 cut(s)	488, 501, 713, 1102, 2662, 3011, 3024
AoxI	GGCC	7 cut(s)	201, 505, 568, 1330, 2863, 2967, 3084
ApaI	GGGCCC	1 cut(s)	509
ApaLI	GTGCAC	1 cut(s)	2718
ApeKI	GCWGC	9 cut(s)	425, 2068, 2318, 2321, 2525, 2780, 3698, 4123, 4145
ApoI	RAATTY	4 cut(s)	17, 435, 1282, 2095
ArsI	GACNNNNNNTTYG	2 cut(s)	2002, 2034
AseI	ATTAAT	1 cut(s)	927
Asp700I	GAANNNNTTC	1 cut(s)	17
AspLEI	GCGC	2 cut(s)	1234, 1314
AsuC2I	CCSGG	1 cut(s)	2359
AsuHPI	GGTGA	7 cut(s)	539, 1383, 1634, 1799, 2438, 3307, 3881
AvaII	GGWCC	5 cut(s)	1168, 1514, 1569, 2575, 2935
BaeGI	GKGCMC	8 cut(s)	366, 509, 806, 1537, 2192, 2255, 2359, 2722
BaeI	ACNNNNGTAYC	2 cut(s)	2865, 2898
BalI	TGGCCA	1 cut(s)	570
BamHI	GGATCC	2 cut(s)	493, 3016
BanI	GGYRCC	2 cut(s)	1532, 4427
BanII	GRGCYC	4 cut(s)	509, 844, 3049, 3439
BarI	GAAGNNNNNNTAC	2 cut(s)	2484, 2516
BauI	CACGAG	1 cut(s)	3860
BbsI	GAAGAC	2 cut(s)	1331, 4046
Bbv12I	GWGCWC	6 cut(s)	1545, 2722, 3049, 3439, 3819, 4073
BbvI	GCAGC	9 cut(s)	412, 2080, 2305, 2308, 2512, 2792, 3710, 4132, 4135
BceAI	ACGGC	1 cut(s)	1317
BciT130I	CCWGG	6 cut(s)	567, 1530, 2514, 3224, 3285, 3456
BciVI	GTATCC	2 cut(s)	2866, 3920
BclI	TGATCA	1 cut(s)	4336
BcnI	CCSGG	1 cut(s)	2359
BcoDI	GTCTC	6 cut(s)	250, 530, 1602, 2003, 2295, 2913
BfaI	CTAG	9 cut(s)	771, 1538, 2409, 2760, 2799, 2930, 3348, 3368, 3653
BfoI	RGCGCY	1 cut(s)	1315
BfuAI	ACCTGC	3 cut(s)	2038, 3620, 3649
BfuI	GTATCC	2 cut(s)	2866, 3920
BlpI	GCTNAGC	1 cut(s)	1581
BmcAI	AGTACT	2 cut(s)	625, 3588
Bme1390I	CCNGG	7 cut(s)	567, 1530, 2359, 2514, 3224, 3285, 3456
Bme18I	GGWCC	5 cut(s)	1168, 1514, 1569, 2575, 2935
BmiI	GGNNCC	9 cut(s)	204, 495, 507, 1534, 2576, 2647, 3018, 3828, 4429
BmrFI	CCNGG	7 cut(s)	567, 1530, 2359, 2514, 3224, 3285, 3456
BmrI	ACTGGG	3 cut(s)	397, 2632, 3912
BmsI	GCATC	9 cut(s)	929, 1696, 1976, 2512, 3397, 3730, 3748, 4212, 4299
BmuI	ACTGGG	3 cut(s)	397, 2632, 3912
BpiI	GAAGAC	2 cut(s)	1331, 4046
BplI	GAGNNNNNCTC	2 cut(s)	2924, 2956
BpmI	CTGGAG	1 cut(s)	3513
Bpu10I	CCTNAGC	1 cut(s)	3081
Bpu1102I	GCTNAGC	1 cut(s)	1581
BpuEI	CTTGAG	4 cut(s)	828, 1594, 1893, 3611
BpuMI	CCSGG	1 cut(s)	2359
BsaBI	GATNNNNATC	4 cut(s)	462, 1697, 3603, 4338
BsaJI	CCNNGG	5 cut(s)	915, 1528, 1727, 3455, 3794
BsaWI	WCCGGW	2 cut(s)	265, 4430
BsaXI	ACNNNNNCTCC	6 cut(s)	2605, 2635, 2970, 3000, 4160, 4190
Bsc4I	CCNNNNNNNGG	8 cut(s)	479, 503, 812, 1298, 1883, 1884, 2198, 3182
Bse1I	ACTGG	7 cut(s)	403, 1170, 1378, 2587, 2638, 2947, 3918
Bse3DI	GCAATG	3 cut(s)	527, 1047, 2190
Bse8I	GATNNNNATC	4 cut(s)	462, 1697, 3603, 4338
BseBI	CCWGG	6 cut(s)	567, 1530, 2514, 3224, 3285, 3456
BseDI	CCNNGG	5 cut(s)	915, 1528, 1727, 3455, 3794
BseJI	GATNNNNATC	4 cut(s)	462, 1697, 3603, 4338
BseLI	CCNNNNNNNGG	8 cut(s)	479, 503, 812, 1298, 1883, 1884, 2198, 3182
BseMI	GCAATG	3 cut(s)	527, 1047, 2190
BseMII	CTCAG	6 cut(s)	1407, 1457, 1572, 1824, 2311, 3657
BseNI	ACTGG	7 cut(s)	403, 1170, 1378, 2587, 2638, 2947, 3918
BseRI	GAGGAG	3 cut(s)	143, 1835, 4181
BseSI	GKGCMC	8 cut(s)	366, 509, 806, 1537, 2192, 2255, 2359, 2722
BseXI	GCAGC	9 cut(s)	412, 2080, 2305, 2308, 2512, 2792, 3710, 4132, 4135
BsgI	GTGCAG	1 cut(s)	4142
Bsh1236I	CGCG	1 cut(s)	1257
BshFI	GGCC	7 cut(s)	203, 507, 570, 1332, 2865, 2969, 3086
BshNI	GGYRCC	2 cut(s)	1532, 4427
BsiHKAI	GWGCWC	6 cut(s)	1545, 2722, 3049, 3439, 3819, 4073
BsiSI	CCGG	3 cut(s)	266, 2359, 4431
BslI	CCNNNNNNNGG	8 cut(s)	479, 503, 812, 1298, 1883, 1884, 2198, 3182
BsmAI	GTCTC	6 cut(s)	250, 530, 1602, 2003, 2295, 2913
BsmI	GAATGC	2 cut(s)	693, 987
BsnI	GGCC	7 cut(s)	203, 507, 570, 1332, 2865, 2969, 3086
Bsp120I	GGGCCC	1 cut(s)	505
Bsp1407I	TGTACA	2 cut(s)	1236, 2401
Bsp1720I	GCTNAGC	1 cut(s)	1581
BspACI	CCGC	7 cut(s)	499, 525, 1257, 1811, 2965, 2970, 3410
BspANI	GGCC	7 cut(s)	203, 507, 570, 1332, 2865, 2969, 3086
BspCNI	CTCAG	6 cut(s)	1408, 1456, 1573, 1825, 2310, 3658
BspFNI	CGCG	1 cut(s)	1257
BspHI	TCATGA	2 cut(s)	903, 1038
BspLI	GGNNCC	9 cut(s)	204, 495, 507, 1534, 2576, 2647, 3018, 3828, 4429
BspMAI	CTGCAG	4 cut(s)	371, 2035, 2557, 2782
BspMI	ACCTGC	3 cut(s)	2038, 3620, 3649
BspPI	GGATC	7 cut(s)	488, 501, 713, 1102, 2662, 3011, 3024
BspQI	GCTCTTC	1 cut(s)	42
BspT107I	GGYRCC	2 cut(s)	1532, 4427
BsrDI	GCAATG	3 cut(s)	527, 1047, 2190
BsrGI	TGTACA	2 cut(s)	1236, 2401
BsrI	ACTGG	7 cut(s)	403, 1170, 1378, 2587, 2638, 2947, 3918
BssECI	CCNNGG	5 cut(s)	915, 1528, 1727, 3455, 3794
BssNAI	GTATAC	2 cut(s)	4179, 4412
BssSI	CACGAG	1 cut(s)	3860
BssT1I	CCWWGG	3 cut(s)	915, 1727, 3794
Bst1107I	GTATAC	2 cut(s)	4179, 4412
Bst2BI	CACGAG	1 cut(s)	3860
Bst2UI	CCWGG	6 cut(s)	567, 1530, 2514, 3224, 3285, 3456
Bst6I	CTCTTC	7 cut(s)	42, 248, 283, 830, 2164, 3899, 3899
BstAUI	TGTACA	2 cut(s)	1236, 2401
BstDEI	CTNAG	9 cut(s)	1416, 1443, 1521, 1581, 1642, 1833, 2297, 3081, 3666
BstFNI	CGCG	1 cut(s)	1257
BstH2I	RGCGCY	1 cut(s)	1315
BstHHI	GCGC	2 cut(s)	1234, 1314
BstMAI	GTCTC	6 cut(s)	250, 530, 1602, 2003, 2295, 2913
BstNI	CCWGG	6 cut(s)	567, 1530, 2514, 3224, 3285, 3456
BstNSI	RCATGY	6 cut(s)	543, 1804, 1808, 2599, 2908, 3093
BstSCI	CCNGG	7 cut(s)	565, 1528, 2357, 2512, 3222, 3283, 3454
BstSLI	GKGCMC	8 cut(s)	366, 509, 806, 1537, 2192, 2255, 2359, 2722
BstUI	CGCG	1 cut(s)	1257
BstV1I	GCAGC	9 cut(s)	412, 2080, 2305, 2308, 2512, 2792, 3710, 4132, 4135
BstV2I	GAAGAC	2 cut(s)	1331, 4046
BstX2I	RGATCY	4 cut(s)	493, 718, 2654, 3016
BstYI	RGATCY	4 cut(s)	493, 718, 2654, 3016
BstZ17I	GTATAC	2 cut(s)	4179, 4412
BsuI	GTATCC	2 cut(s)	2866, 3920
BsuRI	GGCC	7 cut(s)	203, 507, 570, 1332, 2865, 2969, 3086
BtsI	GCAGTG	2 cut(s)	364, 1505
BveI	ACCTGC	3 cut(s)	2038, 3620, 3649
CciI	TCATGA	2 cut(s)	903, 1038
CfoI	GCGC	2 cut(s)	1234, 1314
CsiI	ACCWGGT	1 cut(s)	2512
DdeI	CTNAG	9 cut(s)	1416, 1443, 1521, 1581, 1642, 1833, 2297, 3081, 3666
DraI	TTTAAA	2 cut(s)	886, 3202
DrdI	GACNNNNNNGTC	1 cut(s)	2942
DseDI	GACNNNNNNGTC	1 cut(s)	2942
EaeI	YGGCCR	1 cut(s)	568
Eam1104I	CTCTTC	7 cut(s)	42, 248, 283, 830, 2164, 3899, 3899
EarI	CTCTTC	7 cut(s)	42, 248, 283, 830, 2164, 3899, 3899
Ecl136II	GAGCTC	2 cut(s)	3047, 3437
Eco130I	CCWWGG	3 cut(s)	915, 1727, 3794
Eco147I	AGGCCT	1 cut(s)	2865
Eco24I	GRGCYC	4 cut(s)	509, 844, 3049, 3439
Eco47I	GGWCC	5 cut(s)	1168, 1514, 1569, 2575, 2935
Eco53kI	GAGCTC	2 cut(s)	3047, 3437
Eco57I	CTGAAG	4 cut(s)	2181, 3120, 3377, 4062
EcoICRI	GAGCTC	2 cut(s)	3047, 3437
EcoO109I	RGGNCCY	3 cut(s)	202, 506, 2935
EcoRII	CCWGG	6 cut(s)	565, 1528, 2512, 3222, 3283, 3454
EcoT14I	CCWWGG	3 cut(s)	915, 1727, 3794
EcoT22I	ATGCAT	1 cut(s)	1806
EcoT38I	GRGCYC	4 cut(s)	509, 844, 3049, 3439
ErhI	CCWWGG	3 cut(s)	915, 1727, 3794
FauI	CCCGC	3 cut(s)	506, 2958, 3403
FauNDI	CATATG	3 cut(s)	942, 3703, 4218
FbaI	TGATCA	1 cut(s)	4336
FblI	GTMKAC	3 cut(s)	1021, 4178, 4411
FriOI	GRGCYC	4 cut(s)	509, 844, 3049, 3439
FspBI	CTAG	9 cut(s)	771, 1538, 2409, 2760, 2799, 2930, 3348, 3368, 3653
GlaI	GCGC	2 cut(s)	1233, 1313
GsuI	CTGGAG	1 cut(s)	3513
HaeII	RGCGCY	1 cut(s)	1315
HaeIII	GGCC	7 cut(s)	203, 507, 570, 1332, 2865, 2969, 3086
HapII	CCGG	3 cut(s)	266, 2359, 4431
HhaI	GCGC	2 cut(s)	1234, 1314
Hin6I	GCGC	2 cut(s)	1232, 1312
HinP1I	GCGC	2 cut(s)	1232, 1312
HincII	GTYRAC	2 cut(s)	2696, 4372
HindII	GTYRAC	2 cut(s)	2696, 4372
HindIII	AAGCTT	2 cut(s)	1681, 3716
HpaII	CCGG	3 cut(s)	266, 2359, 4431
HphI	GGTGA	7 cut(s)	539, 1383, 1634, 1799, 2438, 3307, 3881
Hpy166II	GTNNAC	9 cut(s)	634, 768, 1001, 1022, 2696, 2720, 4179, 4372, 4412
Hpy8I	GTNNAC	9 cut(s)	634, 768, 1001, 1022, 2696, 2720, 4179, 4372, 4412
Hpy99I	CGWCG	1 cut(s)	2718
HpyCH4IV	ACGT	6 cut(s)	636, 1212, 1465, 1736, 3952, 4002
HpyF3I	CTNAG	9 cut(s)	1416, 1443, 1521, 1581, 1642, 1833, 2297, 3081, 3666
HpySE526I	ACGT	6 cut(s)	636, 1212, 1465, 1736, 3952, 4002
HspAI	GCGC	2 cut(s)	1232, 1312
Ksp22I	TGATCA	1 cut(s)	4336
LguI	GCTCTTC	1 cut(s)	42
LmnI	GCTCC	5 cut(s)	130, 3044, 3052, 3434, 3551
Lsp1109I	GCAGC	9 cut(s)	412, 2080, 2305, 2308, 2512, 2792, 3710, 4132, 4135
LweI	GCATC	9 cut(s)	929, 1696, 1976, 2512, 3397, 3730, 3748, 4212, 4299
MabI	ACCWGGT	1 cut(s)	2512
MaeI	CTAG	9 cut(s)	771, 1538, 2409, 2760, 2799, 2930, 3348, 3368, 3653
MaeII	ACGT	6 cut(s)	636, 1212, 1465, 1736, 3952, 4002
MfeI	CAATTG	4 cut(s)	858, 2385, 3420, 3945
MflI	RGATCY	4 cut(s)	493, 718, 2654, 3016
MlsI	TGGCCA	1 cut(s)	570
MluNI	TGGCCA	1 cut(s)	570
MlyI	GAGTC	5 cut(s)	65, 904, 3166, 3531, 3697
MmeI	TCCRAC	8 cut(s)	471, 592, 958, 1300, 1402, 2172, 3269, 3614
Mox20I	TGGCCA	1 cut(s)	570
Mph1103I	ATGCAT	1 cut(s)	1806
MroXI	GAANNNNTTC	1 cut(s)	17
MscI	TGGCCA	1 cut(s)	570
MslI	CAYNNNNRTG	6 cut(s)	84, 1052, 1554, 1805, 4403, 4433
Msp20I	TGGCCA	1 cut(s)	570
MspA1I	CMGCKG	2 cut(s)	2879, 3355
MspI	CCGG	3 cut(s)	266, 2359, 4431
MspR9I	CCNGG	7 cut(s)	567, 1530, 2359, 2514, 3224, 3285, 3456
MunI	CAATTG	4 cut(s)	858, 2385, 3420, 3945
Mva1269I	GAATGC	2 cut(s)	693, 987
MvaI	CCWGG	6 cut(s)	567, 1530, 2514, 3224, 3285, 3456
MvnI	CGCG	1 cut(s)	1257
NciI	CCSGG	1 cut(s)	2359
NdeI	CATATG	3 cut(s)	942, 3703, 4218
NlaIV	GGNNCC	9 cut(s)	204, 495, 507, 1534, 2576, 2647, 3018, 3828, 4429
NmuCI	GTSAC	7 cut(s)	527, 739, 1622, 1787, 2411, 2465, 3340
NsiI	ATGCAT	1 cut(s)	1806
NspI	RCATGY	6 cut(s)	543, 1804, 1808, 2599, 2908, 3093
PaeI	GCATGC	1 cut(s)	2599
PagI	TCATGA	2 cut(s)	903, 1038
PaqCI	CACCTGC	1 cut(s)	2038
PceI	AGGCCT	1 cut(s)	2865
PciI	ACATGT	2 cut(s)	539, 2904
PciSI	GCTCTTC	1 cut(s)	42
PctI	GAATGC	2 cut(s)	693, 987
PdmI	GAANNNNTTC	1 cut(s)	17
PfeI	GAWTC	9 cut(s)	911, 1425, 1751, 2335, 2379, 2540, 3274, 4266, 4275
PflMI	CCANNNNNTGG	2 cut(s)	1298, 2198
PfoI	TCCNGGA	1 cut(s)	3222
PleI	GAGTC	5 cut(s)	64, 903, 3166, 3530, 3696
PpsI	GAGTC	5 cut(s)	64, 903, 3166, 3530, 3696
PpuMI	RGGWCCY	1 cut(s)	2935
PscI	ACATGT	2 cut(s)	539, 2904
PshBI	ATTAAT	1 cut(s)	927
PsiI	TTATAA	2 cut(s)	1770, 1937
Psp124BI	GAGCTC	2 cut(s)	3049, 3439
Psp1406I	AACGTT	1 cut(s)	1736
Psp5II	RGGWCCY	1 cut(s)	2935
Psp6I	CCWGG	6 cut(s)	565, 1528, 2512, 3222, 3283, 3454
PspGI	CCWGG	6 cut(s)	565, 1528, 2512, 3222, 3283, 3454
PspN4I	GGNNCC	9 cut(s)	204, 495, 507, 1534, 2576, 2647, 3018, 3828, 4429
PspOMI	GGGCCC	1 cut(s)	505
PspPPI	RGGWCCY	1 cut(s)	2935
PstI	CTGCAG	4 cut(s)	371, 2035, 2557, 2782
PsuI	RGATCY	4 cut(s)	493, 718, 2654, 3016
PvuII	CAGCTG	2 cut(s)	2879, 3355
RseI	CAYNNNNRTG	6 cut(s)	84, 1052, 1554, 1805, 4403, 4433
SacI	GAGCTC	2 cut(s)	3049, 3439
SapI	GCTCTTC	1 cut(s)	42
ScaI	AGTACT	2 cut(s)	625, 3588
SchI	GAGTC	5 cut(s)	65, 904, 3166, 3531, 3697
ScrFI	CCNGG	7 cut(s)	567, 1530, 2359, 2514, 3224, 3285, 3456
SexAI	ACCWGGT	1 cut(s)	2512
SfaNI	GCATC	9 cut(s)	929, 1696, 1976, 2512, 3397, 3730, 3748, 4212, 4299
SinI	GGWCC	5 cut(s)	1168, 1514, 1569, 2575, 2935
SmiMI	CAYNNNNRTG	6 cut(s)	84, 1052, 1554, 1805, 4403, 4433
SmlI	CTYRAG	4 cut(s)	843, 1573, 1908, 3590
SmoI	CTYRAG	4 cut(s)	843, 1573, 1908, 3590
SphI	GCATGC	1 cut(s)	2599
SseBI	AGGCCT	1 cut(s)	2865
SsiI	CCGC	7 cut(s)	499, 525, 1257, 1811, 2965, 2970, 3410
SspI	AATATT	2 cut(s)	709, 1483
SspMI	CTAG	9 cut(s)	771, 1538, 2409, 2760, 2799, 2930, 3348, 3368, 3653
SstI	GAGCTC	2 cut(s)	3049, 3439
StuI	AGGCCT	1 cut(s)	2865
StyD4I	CCNGG	7 cut(s)	565, 1528, 2357, 2512, 3222, 3283, 3454
StyI	CCWWGG	3 cut(s)	915, 1727, 3794
TaiI	ACGT	6 cut(s)	639, 1215, 1468, 1739, 3955, 4005
TaqI	TCGA	5 cut(s)	82, 448, 667, 1828, 2740
TatI	WGTACW	6 cut(s)	623, 767, 1236, 2401, 3586, 4026
TauI	GCSGC	2 cut(s)	1813, 2972
TfiI	GAWTC	9 cut(s)	911, 1425, 1751, 2335, 2379, 2540, 3274, 4266, 4275
TseFI	GTSAC	7 cut(s)	527, 739, 1622, 1787, 2411, 2465, 3340
TseI	GCWGC	9 cut(s)	425, 2068, 2318, 2321, 2525, 2780, 3698, 4123, 4145
Tsp45I	GTSAC	7 cut(s)	527, 739, 1622, 1787, 2411, 2465, 3340
TspGWI	ACGGA	7 cut(s)	1069, 2021, 2204, 2391, 3039, 3634, 4346
Van91I	CCANNNNNTGG	2 cut(s)	1298, 2198
VneI	GTGCAC	1 cut(s)	2718
VpaK11BI	GGWCC	5 cut(s)	1168, 1514, 1569, 2575, 2935
VspI	ATTAAT	1 cut(s)	927
XapI	RAATTY	4 cut(s)	17, 435, 1282, 2095
XbaI	TCTAGA	3 cut(s)	2929, 3347, 3367
XceI	RCATGY	6 cut(s)	543, 1804, 1808, 2599, 2908, 3093
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1646, 2052, 3332
XmiI	GTMKAC	3 cut(s)	1021, 4178, 4411
XmnI	GAANNNNTTC	1 cut(s)	17
XspI	CTAG	9 cut(s)	771, 1538, 2409, 2760, 2799, 2930, 3348, 3368, 3653
ZrmI	AGTACT	2 cut(s)	625, 3588
Zsp2I	ATGCAT	1 cut(s)	1806

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.