AT5G64260

exordium like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002144
Physical Location & Seq
Forward (+)
25703649 .. 25705339
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64260.1

Sequence Viewer

Length: 918 bp
ATGGCTTCTAATTACCGTTTTGCCATCTTCCTCACTCTCTTTTTCGCCACCGCTGGTTTCTCCGCCGCCGCGTTGGTCGAGGAGCAGCCGCTTGTTATGAAATACCACAACGGAGTTCTGTTGAAAGGTAACATCACAGTCAATCTCGTATGGTACGGGAAATTCACACCGATCCAACGGTCCGTAATCGTCGATTTCATCCACTCGCTAAACTCCAAAGACGTTGCATCTTCCGCCGCAGTTCCTTCCGTTGCTTCGTGGTGGAAGACGACGGAGAAATACAAAGGTGGCTCTTCAACACTCGTCGTCGGGAAACAGCTTCTACTCGAGAACTATCCTCTCGGAAAATCTCTCAAAAATCCTTACCTCCGTGCTTTATCCACCAAACTTAACGGCGGTCTCCGTTCCATAACCGTCGTTCTAACGGCGAAAGATGTTACCGTCGAAAGATTCTGTATGAGCCGGTGCGGGACTCACGGATCCTCCGGTTCGAATCCCCGTCGCGCAGCTAACGGCGCGGCTTACGTATGGGTCGGGAACTCCGAGACGCAGTGCCCTGGATATTGCGCGTGGCCGTTTCACCAGCCGATTTACGGACCACAAACGCCGCCGTTAGTAGCGCCTAACGGTGACGTTGGAGTTGACGGAATGATTATAAACCTTGCCACACTTCTAGCTAACACCGTGACGAATCCGTTTAATAACGGATATTACCAAGGCCCACCAACTGCACCGCTTGAAGCTGTGTCTGCTTGTCCTGGTATATTCGGGTCAGGTTCTTATCCGGGTTACGCGGGTCGGGTACTTGTTGACAAAACAACCGGGTCTAGTTACAACGCTCGTGGACTCGCCGGTAGGAAATATCTATTGCCGGCGATGTGGGATCCGCAGAGTTCGACGTGCAAGACTCTGGTTTGA

Protein Analysis

305

Amino Acids

32.67

Weight (kDa)

9.45

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 33 - 304 1.7e-120 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 656
AccII CGCG 5 cut(s) 71, 504, 518, 569, 794
AclWI GGATC 5 cut(s) 166, 474, 487, 878, 891
AcoI YGGCCR 1 cut(s) 572
AcsI RAATTY 1 cut(s) 161
AfaI GTAC 2 cut(s) 155, 804
AfiI CCNNNNNNNGG 1 cut(s) 593
AgsI TTSAA 3 cut(s) 124, 297, 740
AjiI CACGTC 1 cut(s) 900
AjnI CCWGG 2 cut(s) 556, 757
AluBI AGCT 4 cut(s) 319, 509, 677, 743
AluI AGCT 4 cut(s) 319, 509, 677, 743
Alw26I GTCTC 2 cut(s) 404, 539
AlwI GGATC 5 cut(s) 166, 474, 487, 878, 891
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 2 cut(s) 572, 718
ApeKI GCWGC 2 cut(s) 85, 506
ApoI RAATTY 1 cut(s) 161
AspLEI GCGC 4 cut(s) 506, 518, 569, 622
AspS9I GGNCC 3 cut(s) 180, 596, 719
AsuC2I CCSGG 2 cut(s) 786, 823
AsuHPI GGTGA 2 cut(s) 572, 641
AsuII TTCGAA 1 cut(s) 491
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 2 cut(s) 180, 596
BaeGI GKGCMC 1 cut(s) 557
BamHI GGATCC 2 cut(s) 479, 883
BauI CACGAG 1 cut(s) 840
BbsI GAAGAC 1 cut(s) 272
BbvI GCAGC 2 cut(s) 97, 518
BccI CCATC 1 cut(s) 32
BceAI ACGGC 5 cut(s) 409, 441, 529, 559, 595
BciT130I CCWGG 2 cut(s) 558, 759
BcnI CCSGG 2 cut(s) 786, 823
BcoDI GTCTC 2 cut(s) 404, 539
BfaI CTAG 2 cut(s) 674, 828
BfoI RGCGCY 1 cut(s) 623
BisI GCNGC 8 cut(s) 66, 69, 86, 89, 237, 507, 519, 608
BlsI GCNGC 8 cut(s) 67, 70, 87, 90, 238, 508, 520, 609
Bme1390I CCNGG 4 cut(s) 558, 759, 786, 823
Bme18I GGWCC 2 cut(s) 180, 596
BmeT110I CYCGRG 1 cut(s) 326
BmgBI CACGTC 1 cut(s) 900
BmgT120I GGNCC 3 cut(s) 180, 596, 719
BmiI GGNNCC 2 cut(s) 481, 885
BmrFI CCNGG 4 cut(s) 558, 759, 786, 823
BmsI GCATC 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 272
Bpu14I TTCGAA 1 cut(s) 491
BpuMI CCSGG 2 cut(s) 786, 823
BsaAI YACGTR 1 cut(s) 526
BsaI GGTCTC 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 556, 715
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse118I RCCGGY 3 cut(s) 462, 851, 871
BseBI CCWGG 2 cut(s) 558, 759
BseDI CCNNGG 2 cut(s) 556, 715
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 593
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 557
BseXI GCAGC 2 cut(s) 97, 518
BsgI GTGCAG 1 cut(s) 714
Bsh1236I CGCG 5 cut(s) 71, 504, 518, 569, 794
BshFI GGCC 2 cut(s) 574, 720
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 6 cut(s) 463, 486, 785, 822, 852, 872
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 1 cut(s) 593
BsmAI GTCTC 2 cut(s) 404, 539
BsmBI CGTCTC 1 cut(s) 539
BsmFI GGGAC 1 cut(s) 484
BsnI GGCC 2 cut(s) 574, 720
Bso31I GGTCTC 1 cut(s) 404
BsoBI CYCGRG 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 557
Bsp143I GATC 3 cut(s) 171, 479, 883
BspANI GGCC 2 cut(s) 574, 720
BspFNI CGCG 5 cut(s) 71, 504, 518, 569, 794
BspLI GGNNCC 2 cut(s) 481, 885
BspPI GGATC 5 cut(s) 166, 474, 487, 878, 891
BspQI GCTCTTC 1 cut(s) 298
BspT104I TTCGAA 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 404
BsrFI RCCGGY 3 cut(s) 462, 851, 871
BssAI RCCGGY 3 cut(s) 462, 851, 871
BssECI CCNNGG 2 cut(s) 556, 715
BssMI GATC 3 cut(s) 171, 479, 883
BssSI CACGAG 1 cut(s) 840
BssT1I CCWWGG 1 cut(s) 715
Bst2BI CACGAG 1 cut(s) 840
Bst2UI CCWGG 2 cut(s) 558, 759
Bst4CI ACNGT 7 cut(s) 17, 139, 180, 415, 442, 629, 685
Bst6I CTCTTC 1 cut(s) 298
BstBAI YACGTR 1 cut(s) 526
BstBI TTCGAA 1 cut(s) 491
BstC8I GCNNGC 1 cut(s) 873
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 5 cut(s) 71, 504, 518, 569, 794
BstH2I RGCGCY 1 cut(s) 623
BstHHI GCGC 4 cut(s) 506, 518, 569, 622
BstKTI GATC 3 cut(s) 174, 482, 886
BstMAI GTCTC 2 cut(s) 404, 539
BstMBI GATC 3 cut(s) 171, 479, 883
BstMWI GCNNNNNNNGC 3 cut(s) 233, 515, 749
BstNI CCWGG 2 cut(s) 558, 759
BstSCI CCNGG 4 cut(s) 556, 757, 784, 821
BstSLI GKGCMC 1 cut(s) 557
BstSNI TACGTA 1 cut(s) 526
BstUI CGCG 5 cut(s) 71, 504, 518, 569, 794
BstV1I GCAGC 2 cut(s) 97, 518
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 2 cut(s) 479, 883
BstYI RGATCY 2 cut(s) 479, 883
BsuRI GGCC 2 cut(s) 574, 720
BtgZI GCGATG 1 cut(s) 890
BtrI CACGTC 1 cut(s) 900
BtsCI GGATG 1 cut(s) 198
BtsI GCAGTG 1 cut(s) 557
BtsIMutI CAGTG 1 cut(s) 557
Cac8I GCNNGC 1 cut(s) 873
CfoI GCGC 4 cut(s) 506, 518, 569, 622
Cfr10I RCCGGY 3 cut(s) 462, 851, 871
Cfr13I GGNCC 3 cut(s) 180, 596, 719
CpoI CGGWCCG 1 cut(s) 180
CseI GACGC 1 cut(s) 556
Csp6I GTAC 2 cut(s) 154, 803
CspCI CAANNNNNGTGG 2 cut(s) 823, 858
CspI CGGWCCG 1 cut(s) 180
CviQI GTAC 2 cut(s) 154, 803
DpnI GATC 3 cut(s) 173, 481, 885
DpnII GATC 3 cut(s) 171, 479, 883
EaeI YGGCCR 1 cut(s) 572
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
EciI GGCGGA 2 cut(s) 52, 223
Eco105I TACGTA 1 cut(s) 526
Eco130I CCWWGG 1 cut(s) 715
Eco31I GGTCTC 1 cut(s) 404
Eco47I GGWCC 2 cut(s) 180, 596
Eco88I CYCGRG 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 556, 757
EcoT14I CCWWGG 1 cut(s) 715
ErhI CCWWGG 1 cut(s) 715
Esp3I CGTCTC 1 cut(s) 539
FaiI YATR 7 cut(s) 98, 151, 410, 458, 529, 656, 764
FaqI GGGAC 1 cut(s) 484
FauI CCCGC 2 cut(s) 461, 787
Fnu4HI GCNGC 8 cut(s) 66, 69, 86, 89, 237, 507, 519, 608
FokI GGATG 1 cut(s) 185
Fsp4HI GCNGC 8 cut(s) 66, 69, 86, 89, 237, 507, 519, 608
FspBI CTAG 2 cut(s) 674, 828
GlaI GCGC 4 cut(s) 505, 517, 568, 621
GluI GCNGC 8 cut(s) 66, 69, 86, 89, 237, 507, 519, 608
HaeII RGCGCY 1 cut(s) 623
HaeIII GGCC 2 cut(s) 574, 720
HapII CCGG 6 cut(s) 463, 486, 785, 822, 852, 872
HgaI GACGC 1 cut(s) 556
HhaI GCGC 4 cut(s) 506, 518, 569, 622
Hin6I GCGC 4 cut(s) 504, 516, 567, 620
HinP1I GCGC 4 cut(s) 504, 516, 567, 620
HincII GTYRAC 2 cut(s) 643, 811
HindII GTYRAC 2 cut(s) 643, 811
HinfI GANTC 6 cut(s) 450, 472, 493, 691, 846, 907
HpaII CCGG 6 cut(s) 463, 486, 785, 822, 852, 872
HphI GGTGA 2 cut(s) 572, 641
Hpy166II GTNNAC 3 cut(s) 643, 811, 845
Hpy188I TCNGA 2 cut(s) 344, 544
Hpy188III TCNNGA 3 cut(s) 310, 328, 535
Hpy8I GTNNAC 3 cut(s) 643, 811, 845
Hpy99I CGWCG 8 cut(s) 194, 274, 308, 311, 419, 446, 504, 901
HpyAV CCTTC 1 cut(s) 255
HpyCH4III ACNGT 7 cut(s) 17, 139, 180, 415, 442, 629, 685
HpyCH4IV ACGT 4 cut(s) 222, 525, 633, 899
HpyCH4V TGCA 3 cut(s) 227, 731, 903
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 515, 749
HpySE526I ACGT 4 cut(s) 222, 525, 633, 899
HspAI GCGC 4 cut(s) 504, 516, 567, 620
KroI GCCGGC 1 cut(s) 871
KroNI GCCGGC 1 cut(s) 873
Kzo9I GATC 3 cut(s) 171, 479, 883
LguI GCTCTTC 1 cut(s) 298
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 2 cut(s) 97, 518
LweI GCATC 1 cut(s) 236
MaeI CTAG 2 cut(s) 674, 828
MaeII ACGT 4 cut(s) 222, 525, 633, 899
MaeIII GTNAC 6 cut(s) 128, 436, 629, 685, 788, 830
MalI GATC 3 cut(s) 173, 481, 885
MboI GATC 3 cut(s) 171, 479, 883
MboII GAAGA 4 cut(s) 19, 222, 277, 285
MflI RGATCY 2 cut(s) 479, 883
MhlI GDGCHC 1 cut(s) 557
MluCI AATT 2 cut(s) 10, 161
MlyI GAGTC 3 cut(s) 466, 840, 901
MmeI TCCRAC 2 cut(s) 199, 616
MnlI CCTC 5 cut(s) 41, 73, 348, 377, 493
MroNI GCCGGC 1 cut(s) 871
MseI TTAA 2 cut(s) 390, 699
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 6 cut(s) 463, 486, 785, 822, 852, 872
MspR9I CCNGG 4 cut(s) 558, 759, 786, 823
MvaI CCWGG 2 cut(s) 558, 759
MvnI CGCG 5 cut(s) 71, 504, 518, 569, 794
MwoI GCNNNNNNNGC 3 cut(s) 233, 515, 749
NaeI GCCGGC 1 cut(s) 873
NciI CCSGG 2 cut(s) 786, 823
NdeII GATC 3 cut(s) 171, 479, 883
NgoMIV GCCGGC 1 cut(s) 871
NlaIV GGNNCC 2 cut(s) 481, 885
NmuCI GTSAC 2 cut(s) 629, 685
NspV TTCGAA 1 cut(s) 491
PaeR7I CTCGAG 1 cut(s) 326
PciSI GCTCTTC 1 cut(s) 298
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PdiI GCCGGC 1 cut(s) 873
PfeI GAWTC 3 cut(s) 450, 493, 691
PkrI GCNGC 8 cut(s) 67, 70, 87, 90, 238, 508, 520, 609
PleI GAGTC 3 cut(s) 466, 840, 901
PpsI GAGTC 3 cut(s) 466, 840, 901
Ppu21I YACGTR 1 cut(s) 526
PsiI TTATAA 1 cut(s) 656
Psp6I CCWGG 2 cut(s) 556, 757
PspGI CCWGG 2 cut(s) 556, 757
PspN4I GGNNCC 2 cut(s) 481, 885
PspPI GGNCC 3 cut(s) 180, 596, 719
PsuI RGATCY 2 cut(s) 479, 883
RsaI GTAC 2 cut(s) 155, 804
RsaNI GTAC 2 cut(s) 154, 803
Rsr2I CGGWCCG 1 cut(s) 180
RsrII CGGWCCG 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 298
SaqAI TTAA 2 cut(s) 390, 699
SatI GCNGC 8 cut(s) 66, 69, 86, 89, 237, 507, 519, 608
Sau3AI GATC 3 cut(s) 171, 479, 883
Sau96I GGNCC 3 cut(s) 180, 596, 719
SchI GAGTC 3 cut(s) 466, 840, 901
ScrFI CCNGG 4 cut(s) 558, 759, 786, 823
SduI GDGCHC 1 cut(s) 557
SfaNI GCATC 1 cut(s) 236
Sfr274I CTCGAG 1 cut(s) 326
SfuI TTCGAA 1 cut(s) 491
SinI GGWCC 2 cut(s) 180, 596
SlaI CTCGAG 1 cut(s) 326
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SnaBI TACGTA 1 cut(s) 526
Sse9I AATT 2 cut(s) 10, 161
SspMI CTAG 2 cut(s) 674, 828
StyD4I CCNGG 4 cut(s) 556, 757, 784, 821
StyI CCWWGG 1 cut(s) 715
TaaI ACNGT 7 cut(s) 17, 139, 180, 415, 442, 629, 685
TaiI ACGT 4 cut(s) 225, 528, 636, 902
TaqI TCGA 6 cut(s) 78, 192, 327, 444, 491, 896
TasI AATT 2 cut(s) 10, 161
TauI GCSGC 6 cut(s) 68, 71, 91, 239, 521, 610
TfiI GAWTC 3 cut(s) 450, 493, 691
Tru1I TTAA 2 cut(s) 390, 699
Tru9I TTAA 2 cut(s) 390, 699
TscAI CASTG 1 cut(s) 557
TseFI GTSAC 2 cut(s) 629, 685
TseI GCWGC 2 cut(s) 85, 506
Tsp45I GTSAC 2 cut(s) 629, 685
TspDTI ATGAA 2 cut(s) 113, 187
TspRI CASTG 1 cut(s) 557
VpaK11BI GGWCC 2 cut(s) 180, 596
XapI RAATTY 1 cut(s) 161
XhoI CTCGAG 1 cut(s) 326
XspI CTAG 2 cut(s) 674, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.