Rroxscaffold_3G00222110

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002144
Physical Location & Seq
Forward (+)
5101875 .. 5102798
924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00222110.1

Sequence Viewer

Length: 924 bp
ATGGCTTATAATTACCATTTGGCCATCTGGGTCTTCTTCCTCCTCGCACTCTCGACCTTGCTCCGCCCTAGTGCAGCCACACCTCGAAAGCTCTCGGCCCTCGTCCAACAACAACCCCTGGTCCTCAAGTACCACAAGGGCCCACTTCTCCATGGCAACGTCACCGTAAACCTCGTATGGTACGGTAAATTCTCCCCCAATCAACGGTCAATTCTCGTTGACTTTCTTCAGTCTCTGAGCTCCGGCCACCGCGCTCCACAGCCCTCGGTCTCGTCGTGGTGGCAGACCATTGCGGGGTACATGCGCGGCCCATGCACTGTCGCTGTAGGGAATCAAATCCTCCAGCAGAGCTACTCCCTCGGCAAAACACTCACGAACCAAAAGCTCGCGGTTCTCGCGGCGAAAGGATCAAGGGGCGGTCGTAAAACGGCGGCGGTCAACGTCGTTTTGACGGACGCTGACGTGGCGGTGGAGGGGTTCTGCATGAGCCGGTGCGGGAGTCACGGGTCGAGTCGGGGCGGGTCGGCGTACGCGTGGGTGGGGAACCCGGGGAAGCAGTGTCCCGGTCAGTGCGCGTGGCCGTTTCACCAGCCGATTTACGGGCCACAGACTCCTCCGTTAGTTTCGCCAAACGGCGACGTCGGGGTAGACGGAATGGTGATCAATCTGGCTACGGTTTTGGCGGGAACGGTAACGAATCCGTTTGATAACGGGTACTTCCAGGGAGCGGCAGGCGCGGGTTTGGAGGCCGTGAGCGCGTGCACGGGTATTTTCGGGAAAGGGGCGTACCCTGGTTATCCGGGGGAGGTCGTTTTGGACAAAACGACGGGAGCGAGTTACAATGCGGTTGGAGTACGTGGACGGAAGTATCTGCTTCCGGCGATGTGGGACCCGAAGACATCCGCGTGTAAAACACTCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

307

Amino Acids

32.5

Weight (kDa)

9.58

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 42 - 306 3.1e-119 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 9
AatII GACGTC 1 cut(s) 642
AccBSI CCGCTC 1 cut(s) 728
AccI GTMKAC 1 cut(s) 648
AccII CGCG 9 cut(s) 252, 306, 389, 398, 533, 575, 737, 758, 905
AclWI GGATC 1 cut(s) 415
AcoI YGGCCR 3 cut(s) 21, 244, 578
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 212
AcyI GRCGYC 1 cut(s) 639
AfaI GTAC 7 cut(s) 131, 182, 299, 530, 716, 788, 855
AfiI CCNNNNNNNGG 4 cut(s) 204, 294, 599, 727
AflIII ACRYGT 1 cut(s) 531
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 3 cut(s) 117, 720, 790
AluBI AGCT 4 cut(s) 91, 240, 351, 385
AluI AGCT 4 cut(s) 91, 240, 351, 385
Alw21I GWGCWC 2 cut(s) 242, 764
Alw26I GTCTC 2 cut(s) 237, 274
Alw44I GTGCAC 1 cut(s) 760
AlwI GGATC 1 cut(s) 415
AlwNI CAGNNNCTG 1 cut(s) 235
Ama87I CYCGRG 1 cut(s) 547
AoxI GGCC 8 cut(s) 21, 96, 139, 244, 307, 578, 602, 747
ApaI GGGCCC 1 cut(s) 143
ApaLI GTGCAC 1 cut(s) 760
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 1 cut(s) 188
AspLEI GCGC 5 cut(s) 254, 306, 575, 737, 758
AspS9I GGNCC 7 cut(s) 97, 121, 139, 140, 308, 602, 889
AsuC2I CCSGG 4 cut(s) 548, 549, 564, 801
AsuHPI GGTGA 3 cut(s) 154, 578, 670
AvaI CYCGRG 1 cut(s) 547
AvaII GGWCC 2 cut(s) 121, 889
BaeGI GKGCMC 2 cut(s) 143, 764
BaeI ACNNNNGTAYC 2 cut(s) 851, 884
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 2 cut(s) 143, 242
BbsI GAAGAC 2 cut(s) 25, 902
Bbv12I GWGCWC 2 cut(s) 242, 764
BbvI GCAGC 1 cut(s) 86
BccI CCATC 1 cut(s) 32
BceAI ACGGC 4 cut(s) 444, 565, 649, 734
BciT130I CCWGG 3 cut(s) 119, 722, 792
BclI TGATCA 1 cut(s) 660
BcnI CCSGG 4 cut(s) 548, 549, 564, 801
BcoDI GTCTC 2 cut(s) 237, 274
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 1 cut(s) 324
BisI GCNGC 5 cut(s) 75, 307, 399, 432, 729
BlsI GCNGC 5 cut(s) 76, 308, 400, 433, 730
Bme1390I CCNGG 7 cut(s) 119, 548, 549, 564, 722, 792, 801
Bme18I GGWCC 2 cut(s) 121, 889
BmeT110I CYCGRG 1 cut(s) 547
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 7 cut(s) 97, 121, 139, 140, 308, 602, 889
BmiI GGNNCC 4 cut(s) 141, 545, 890, 891
BmrFI CCNGG 7 cut(s) 119, 548, 549, 564, 722, 792, 801
BpiI GAAGAC 2 cut(s) 25, 902
BpmI CTGGAG 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 4 cut(s) 548, 549, 564, 801
BsaAI YACGTR 1 cut(s) 857
BsaHI GRCGYC 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 274
BsaJI CCNNGG 9 cut(s) 117, 151, 264, 358, 547, 548, 721, 790, 800
Bsc4I CCNNNNNNNGG 4 cut(s) 204, 294, 599, 727
Bse118I RCCGGY 1 cut(s) 489
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 3 cut(s) 119, 722, 792
BseDI CCNNGG 9 cut(s) 117, 151, 264, 358, 547, 548, 721, 790, 800
BseGI GGATG 1 cut(s) 899
BseLI CCNNNNNNNGG 4 cut(s) 204, 294, 599, 727
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 227
BseRI GAGGAG 2 cut(s) 32, 603
BseSI GKGCMC 2 cut(s) 143, 764
BseXI GCAGC 1 cut(s) 86
BsgI GTGCAG 1 cut(s) 93
Bsh1236I CGCG 9 cut(s) 252, 306, 389, 398, 533, 575, 737, 758, 905
Bsh1285I CGRYCG 1 cut(s) 421
BshFI GGCC 8 cut(s) 23, 98, 141, 246, 309, 580, 604, 749
BsiEI CGRYCG 1 cut(s) 421
BsiHKAI GWGCWC 2 cut(s) 242, 764
BsiHKCI CYCGRG 1 cut(s) 547
BsiSI CCGG 6 cut(s) 243, 490, 548, 564, 800, 878
BsiWI CGTACG 1 cut(s) 528
BslFI GGGAC 2 cut(s) 546, 902
BslI CCNNNNNNNGG 4 cut(s) 204, 294, 599, 727
BsmAI GTCTC 2 cut(s) 237, 274
BsmFI GGGAC 2 cut(s) 546, 902
BsnI GGCC 8 cut(s) 23, 98, 141, 246, 309, 580, 604, 749
Bso31I GGTCTC 1 cut(s) 274
BsoBI CYCGRG 1 cut(s) 547
Bsp120I GGGCCC 1 cut(s) 139
Bsp1286I GDGCHC 3 cut(s) 143, 242, 764
Bsp143I GATC 2 cut(s) 407, 660
Bsp19I CCATGG 1 cut(s) 151
BspANI GGCC 8 cut(s) 23, 98, 141, 246, 309, 580, 604, 749
BspCNI CTCAG 1 cut(s) 228
BspFNI CGCG 9 cut(s) 252, 306, 389, 398, 533, 575, 737, 758, 905
BspLI GGNNCC 4 cut(s) 141, 545, 890, 891
BspPI GGATC 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 274
BsrBI CCGCTC 1 cut(s) 728
BsrDI GCAATG 1 cut(s) 288
BsrFI RCCGGY 1 cut(s) 489
BssAI RCCGGY 1 cut(s) 489
BssECI CCNNGG 9 cut(s) 117, 151, 264, 358, 547, 548, 721, 790, 800
BssMI GATC 2 cut(s) 407, 660
BssNI GRCGYC 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 3 cut(s) 119, 722, 792
Bst4CI ACNGT 6 cut(s) 166, 185, 207, 319, 676, 691
BstACI GRCGYC 1 cut(s) 639
BstBAI YACGTR 1 cut(s) 857
BstC8I GCNNGC 3 cut(s) 387, 733, 760
BstDEI CTNAG 1 cut(s) 236
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 1 cut(s) 899
BstFNI CGCG 9 cut(s) 252, 306, 389, 398, 533, 575, 737, 758, 905
BstHHI GCGC 5 cut(s) 254, 306, 575, 737, 758
BstKTI GATC 2 cut(s) 410, 663
BstMAI GTCTC 2 cut(s) 237, 274
BstMBI GATC 2 cut(s) 407, 660
BstMCI CGRYCG 1 cut(s) 421
BstMWI GCNNNNNNNGC 5 cut(s) 312, 395, 464, 734, 755
BstNI CCWGG 3 cut(s) 119, 722, 792
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 7 cut(s) 117, 546, 547, 562, 720, 790, 799
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 2 cut(s) 143, 764
BstUI CGCG 9 cut(s) 252, 306, 389, 398, 533, 575, 737, 758, 905
BstV1I GCAGC 1 cut(s) 86
BstV2I GAAGAC 2 cut(s) 25, 902
BsuRI GGCC 8 cut(s) 23, 98, 141, 246, 309, 580, 604, 749
BtgI CCRYGG 1 cut(s) 151
BtgZI GCGATG 1 cut(s) 896
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 1 cut(s) 899
BtsI GCAGTG 1 cut(s) 563
BtsIMutI CAGTG 3 cut(s) 315, 563, 575
Cac8I GCNNGC 3 cut(s) 387, 733, 760
CaiI CAGNNNCTG 1 cut(s) 235
CfoI GCGC 5 cut(s) 254, 306, 575, 737, 758
Cfr10I RCCGGY 1 cut(s) 489
Cfr13I GGNCC 7 cut(s) 97, 121, 139, 140, 308, 602, 889
Cfr9I CCCGGG 1 cut(s) 547
CseI GACGC 1 cut(s) 464
Csp6I GTAC 7 cut(s) 130, 181, 298, 529, 715, 787, 854
CviAII CATG 4 cut(s) 152, 301, 312, 484
CviQI GTAC 7 cut(s) 130, 181, 298, 529, 715, 787, 854
DdeI CTNAG 1 cut(s) 236
DpnI GATC 2 cut(s) 409, 662
DpnII GATC 2 cut(s) 407, 660
EaeI YGGCCR 3 cut(s) 21, 244, 578
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 151
Eco24I GRGCYC 2 cut(s) 143, 242
Eco31I GGTCTC 1 cut(s) 274
Eco47I GGWCC 2 cut(s) 121, 889
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 547
EcoICRI GAGCTC 1 cut(s) 240
EcoO109I RGGNCCY 2 cut(s) 139, 889
EcoRII CCWGG 3 cut(s) 117, 720, 790
EcoT14I CCWWGG 1 cut(s) 151
EcoT38I GRGCYC 2 cut(s) 143, 242
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 4 cut(s) 155, 304, 315, 487
FaiI YATR 6 cut(s) 9, 153, 178, 302, 313, 485
FaqI GGGAC 2 cut(s) 546, 902
FatI CATG 4 cut(s) 151, 300, 311, 483
FauI CCCGC 5 cut(s) 286, 488, 512, 676, 730
FbaI TGATCA 1 cut(s) 660
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 5 cut(s) 75, 307, 399, 432, 729
FokI GGATG 1 cut(s) 886
FriOI GRGCYC 2 cut(s) 143, 242
Fsp4HI GCNGC 5 cut(s) 75, 307, 399, 432, 729
FspBI CTAG 1 cut(s) 69
GlaI GCGC 5 cut(s) 253, 305, 574, 736, 757
GluI GCNGC 5 cut(s) 75, 307, 399, 432, 729
GsuI CTGGAG 1 cut(s) 326
HaeIII GGCC 8 cut(s) 23, 98, 141, 246, 309, 580, 604, 749
HapII CCGG 6 cut(s) 243, 490, 548, 564, 800, 878
HgaI GACGC 1 cut(s) 464
HhaI GCGC 5 cut(s) 254, 306, 575, 737, 758
Hin1I GRCGYC 1 cut(s) 639
Hin1II CATG 4 cut(s) 155, 304, 315, 487
Hin6I GCGC 5 cut(s) 252, 304, 573, 735, 756
HinP1I GCGC 5 cut(s) 252, 304, 573, 735, 756
HincII GTYRAC 2 cut(s) 220, 439
HindII GTYRAC 2 cut(s) 220, 439
HinfI GANTC 5 cut(s) 331, 499, 511, 610, 697
HpaII CCGG 6 cut(s) 243, 490, 548, 564, 800, 878
HphI GGTGA 3 cut(s) 154, 578, 670
Hpy166II GTNNAC 6 cut(s) 169, 220, 439, 649, 762, 860
Hpy188I TCNGA 2 cut(s) 237, 923
Hpy188III TCNNGA 3 cut(s) 52, 373, 775
Hpy8I GTNNAC 6 cut(s) 169, 220, 439, 649, 762, 860
Hpy99I CGWCG 5 cut(s) 277, 446, 641, 644, 829
HpyCH4III ACNGT 6 cut(s) 166, 185, 207, 319, 676, 691
HpyCH4IV ACGT 5 cut(s) 159, 441, 462, 639, 856
HpyCH4V TGCA 4 cut(s) 74, 315, 483, 762
HpyF10VI GCNNNNNNNGC 5 cut(s) 312, 395, 464, 734, 755
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 5 cut(s) 159, 441, 462, 639, 856
Hsp92I GRCGYC 1 cut(s) 639
Hsp92II CATG 4 cut(s) 155, 304, 315, 487
HspAI GCGC 5 cut(s) 252, 304, 573, 735, 756
KflI GGGWCCC 1 cut(s) 889
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 2 cut(s) 407, 660
LmnI GCTCC 5 cut(s) 66, 245, 259, 725, 830
Lsp1109I GCAGC 1 cut(s) 86
MaeI CTAG 1 cut(s) 69
MaeII ACGT 5 cut(s) 159, 441, 462, 639, 856
MaeIII GTNAC 4 cut(s) 160, 500, 691, 836
MalI GATC 2 cut(s) 409, 662
MbiI CCGCTC 1 cut(s) 728
MboI GATC 2 cut(s) 407, 660
MboII GAAGA 4 cut(s) 25, 28, 218, 907
MhlI GDGCHC 3 cut(s) 143, 242, 764
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 3 cut(s) 10, 188, 210
MluI ACGCGT 1 cut(s) 531
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 3 cut(s) 508, 520, 604
MmeI TCCRAC 2 cut(s) 130, 829
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
MslI CAYNNNNRTG 1 cut(s) 904
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 6 cut(s) 243, 490, 548, 564, 800, 878
MspR9I CCNGG 7 cut(s) 119, 548, 549, 564, 722, 792, 801
MvaI CCWGG 3 cut(s) 119, 722, 792
MvnI CGCG 9 cut(s) 252, 306, 389, 398, 533, 575, 737, 758, 905
MwoI GCNNNNNNNGC 5 cut(s) 312, 395, 464, 734, 755
NciI CCSGG 4 cut(s) 548, 549, 564, 801
NcoI CCATGG 1 cut(s) 151
NdeII GATC 2 cut(s) 407, 660
NlaIII CATG 4 cut(s) 155, 304, 315, 487
NlaIV GGNNCC 4 cut(s) 141, 545, 890, 891
NmeAIII GCCGAG 2 cut(s) 74, 339
NmuCI GTSAC 2 cut(s) 160, 500
NspI RCATGY 1 cut(s) 304
PcsI WCGNNNNNNNCGW 3 cut(s) 272, 530, 830
PfeI GAWTC 2 cut(s) 331, 697
Pfl23II CGTACG 1 cut(s) 528
PkrI GCNGC 5 cut(s) 76, 308, 400, 433, 730
PleI GAGTC 3 cut(s) 507, 519, 604
PpsI GAGTC 3 cut(s) 507, 519, 604
Ppu21I YACGTR 1 cut(s) 857
PpuMI RGGWCCY 1 cut(s) 889
PsiI TTATAA 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 889
Psp6I CCWGG 3 cut(s) 117, 720, 790
PspGI CCWGG 3 cut(s) 117, 720, 790
PspLI CGTACG 1 cut(s) 528
PspN4I GGNNCC 4 cut(s) 141, 545, 890, 891
PspOMI GGGCCC 1 cut(s) 139
PspPI GGNCC 7 cut(s) 97, 121, 139, 140, 308, 602, 889
PspPPI RGGWCCY 1 cut(s) 889
PstNI CAGNNNCTG 1 cut(s) 235
RsaI GTAC 7 cut(s) 131, 182, 299, 530, 716, 788, 855
RsaNI GTAC 7 cut(s) 130, 181, 298, 529, 715, 787, 854
RseI CAYNNNNRTG 1 cut(s) 904
SacI GAGCTC 1 cut(s) 242
SatI GCNGC 5 cut(s) 75, 307, 399, 432, 729
Sau3AI GATC 2 cut(s) 407, 660
Sau96I GGNCC 7 cut(s) 97, 121, 139, 140, 308, 602, 889
SchI GAGTC 3 cut(s) 508, 520, 604
ScrFI CCNGG 7 cut(s) 119, 548, 549, 564, 722, 792, 801
SduI GDGCHC 3 cut(s) 143, 242, 764
SfcI CTRYAG 1 cut(s) 324
SinI GGWCC 2 cut(s) 121, 889
SmaI CCCGGG 1 cut(s) 549
SmiMI CAYNNNNRTG 1 cut(s) 904
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 3 cut(s) 10, 188, 210
SspMI CTAG 1 cut(s) 69
SstI GAGCTC 1 cut(s) 242
StyD4I CCNGG 7 cut(s) 117, 546, 547, 562, 720, 790, 799
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 6 cut(s) 166, 185, 207, 319, 676, 691
TaiI ACGT 5 cut(s) 162, 444, 465, 642, 859
TaqI TCGA 3 cut(s) 53, 85, 509
TaqII GACCGA 1 cut(s) 256
TasI AATT 3 cut(s) 10, 188, 210
TauI GCSGC 4 cut(s) 309, 401, 434, 731
TfiI GAWTC 2 cut(s) 331, 697
TscAI CASTG 3 cut(s) 322, 563, 575
TseFI GTSAC 2 cut(s) 160, 500
TseI GCWGC 1 cut(s) 74
Tsp45I GTSAC 2 cut(s) 160, 500
TspGWI ACGGA 5 cut(s) 467, 606, 666, 690, 877
TspMI CCCGGG 1 cut(s) 547
TspRI CASTG 3 cut(s) 322, 563, 575
VneI GTGCAC 1 cut(s) 760
VpaK11BI GGWCC 2 cut(s) 121, 889
XapI RAATTY 1 cut(s) 188
XceI RCATGY 1 cut(s) 304
XmaI CCCGGG 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 648
XspI CTAG 1 cut(s) 69
ZraI GACGTC 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.