pycom05g03550

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002144
Physical Location & Seq
Reverse (-)
4106533 .. 4107675
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g03550.1

Sequence Viewer

Length: 1050 bp
ATGAATAACATTATTACGATAAATCTTACACTGTCTTCCTTATCGTCCGGATATAAATACCAACCCAAACCAAATTCACCAAATCGTCCTCCCTCTCTCTCTCTCTTCCCCCCCCCCCCCTTCGCCCTACTCCCGAAGAGAAGCCTTAAATCCTCACCGTCCTTCGGAATGTATCCGATTTACCATTTTGCCACTGCTCTCTTGCTCCTCCTTTTGGTGGCGAAGCCCGGTTTTGGCGCTTTAGTGGAGGAGCAGCCACTGGTGCTCAAGTACCACAACGGCGCTCTTCTTAAGGGCAACATCACCGTCAATCTTATTTGGTACGGCAGATTCACCCCTATCCAACGGTCCATCATCATCGACTTCATCCACTCCCTCGCCCCCCGGCGTTCCCCACTCCCCTCCGCCTCCTCCTGGTGGAAAACCACCGAGAAGTACAGGGGCGGCGCCACCAATCTCGTCGTCGGCAGGCAAATCCTCCACGAGGCCTACTCTCTCGGAAAGTCCCTCCGTAACCGACACCTGGTGGCCCTCGCCGGCAAAGTCAATGCGTTGAACTCCATCAACGTTGTTTTGACTGCCAGTGACGTGGCGGTCGACGGTTTCTGTAGCCGCTGCGGGACCCACGGCTCCAACCGGGACAAAAAGACGGCTTACGTGTGGGTCGGGAACTCGGTGGTCCAGTGCCCCGGCCAATGCGCCTGGCCCTTTCACCAGCCCATTTACGGCCCGCAAACTCCGCCGTTAGTGGCTCCGAACGGCGACGTCGGAGTTGATGGCATGATCATAAACATCGCCACTCTCTTGGCGGGAACCGTAACCAATCCGTACAACAATGGGTACTTCCAAGGGCCGGCCAGCGCGCCGCTCGAGGCCGTTTCTGCCTGCACCGGCGTTTTCGGAACCGGGGCCTACCCCGGGTATCCGGGTCGGGTCCTGGTAGATAAGGCGACCGGGGCGAGCTATAACGCGGTTGGGGTAAACGGGCGTAGGTTCCTATTGCCAGCCATGTGGGACCCGCAAACCTCTGCATGCAAGCCGCTTGTGTGA

Protein Analysis

350

Amino Acids

37.5

Weight (kDa)

9.75

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 89 - 346 3.2e-118 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 593
AatII GACGTC 1 cut(s) 768
AccB1I GGYRCC 1 cut(s) 446
AccBSI CCGCTC 1 cut(s) 868
AccI GTMKAC 1 cut(s) 597
AccII CGCG 2 cut(s) 863, 971
AccIII TCCGGA 1 cut(s) 47
AclI AACGTT 1 cut(s) 567
AcoI YGGCCR 2 cut(s) 691, 855
AcsI RAATTY 1 cut(s) 73
AcyI GRCGYC 2 cut(s) 447, 765
AdeI CACNNNGTG 1 cut(s) 526
AfaI GTAC 5 cut(s) 272, 323, 437, 830, 842
AflII CTTAAG 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 657
AgsI TTSAA 1 cut(s) 556
AjiI CACGTC 1 cut(s) 589
AjnI CCWGG 4 cut(s) 413, 522, 701, 936
AluBI AGCT 1 cut(s) 963
AluI AGCT 1 cut(s) 963
Alw21I GWGCWC 1 cut(s) 267
AlwNI CAGNNNCTG 1 cut(s) 259
Ama87I CYCGRG 2 cut(s) 869, 917
Aor13HI TCCGGA 1 cut(s) 47
AoxI GGCC 9 cut(s) 486, 528, 691, 704, 727, 851, 855, 873, 909
ApeKI GCWGC 2 cut(s) 253, 615
ApoI RAATTY 1 cut(s) 73
AspLEI GCGC 6 cut(s) 239, 284, 449, 701, 863, 865
AsuC2I CCSGG 9 cut(s) 228, 385, 638, 690, 907, 918, 919, 927, 955
AsuHPI GGTGA 5 cut(s) 69, 147, 295, 325, 704
AvaI CYCGRG 2 cut(s) 869, 917
AvaII GGWCC 5 cut(s) 348, 621, 679, 934, 1015
BaeGI GKGCMC 1 cut(s) 689
BanI GGYRCC 1 cut(s) 446
BauI CACGAG 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 265, 602
BccI CCATC 3 cut(s) 359, 569, 770
BceAI ACGGC 8 cut(s) 295, 340, 643, 666, 727, 742, 775, 860
BciT130I CCWGG 4 cut(s) 415, 524, 703, 938
BciVI GTATCC 2 cut(s) 183, 933
BclI TGATCA 1 cut(s) 783
BcnI CCSGG 9 cut(s) 228, 385, 638, 690, 907, 918, 919, 927, 955
BfmI CTRYAG 1 cut(s) 607
BfoI RGCGCY 3 cut(s) 240, 285, 450
BfrI CTTAAG 1 cut(s) 290
BfuI GTATCC 2 cut(s) 183, 933
BisI GCNGC 6 cut(s) 254, 445, 613, 616, 866, 1040
BlsI GCNGC 6 cut(s) 255, 446, 614, 617, 867, 1041
Bme18I GGWCC 5 cut(s) 348, 621, 679, 934, 1015
BmeT110I CYCGRG 2 cut(s) 869, 917
BmgBI CACGTC 1 cut(s) 589
BpiI GAAGAC 1 cut(s) 27
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpuEI CTTGAG 1 cut(s) 251
BpuMI CCSGG 9 cut(s) 228, 385, 638, 690, 907, 918, 919, 927, 955
BsaAI YACGTR 1 cut(s) 658
BsaHI GRCGYC 2 cut(s) 447, 765
BsaJI CCNNGG 8 cut(s) 383, 625, 688, 847, 906, 916, 917, 954
BsaWI WCCGGW 1 cut(s) 47
Bse118I RCCGGY 3 cut(s) 536, 853, 890
Bse1I ACTGG 3 cut(s) 264, 582, 682
BseAI TCCGGA 1 cut(s) 47
BseBI CCWGG 4 cut(s) 415, 524, 703, 938
BseDI CCNNGG 8 cut(s) 383, 625, 688, 847, 906, 916, 917, 954
BseGI GGATG 1 cut(s) 366
BseNI ACTGG 3 cut(s) 264, 582, 682
BsePI GCGCGC 1 cut(s) 861
BseRI GAGGAG 3 cut(s) 197, 263, 400
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 2 cut(s) 265, 602
BsgI GTGCAG 1 cut(s) 871
Bsh1236I CGCG 2 cut(s) 863, 971
Bsh1285I CGRYCG 2 cut(s) 597, 954
BshFI GGCC 9 cut(s) 488, 530, 693, 706, 729, 853, 857, 875, 911
BshNI GGYRCC 1 cut(s) 446
BsiEI CGRYCG 2 cut(s) 597, 954
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 2 cut(s) 869, 917
BslFI GGGAC 4 cut(s) 490, 634, 653, 1028
BsmFI GGGAC 4 cut(s) 490, 634, 653, 1028
BsnI GGCC 9 cut(s) 488, 530, 693, 706, 729, 853, 857, 875, 911
BsoBI CYCGRG 2 cut(s) 869, 917
Bsp1286I GDGCHC 2 cut(s) 267, 689
Bsp13I TCCGGA 1 cut(s) 47
Bsp143I GATC 1 cut(s) 783
BspANI GGCC 9 cut(s) 488, 530, 693, 706, 729, 853, 857, 875, 911
BspEI TCCGGA 1 cut(s) 47
BspFNI CGCG 2 cut(s) 863, 971
BspQI GCTCTTC 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 446
BspTI CTTAAG 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 868
BsrFI RCCGGY 3 cut(s) 536, 853, 890
BsrI ACTGG 3 cut(s) 264, 582, 682
BssAI RCCGGY 3 cut(s) 536, 853, 890
BssECI CCNNGG 8 cut(s) 383, 625, 688, 847, 906, 916, 917, 954
BssHII GCGCGC 1 cut(s) 861
BssMI GATC 1 cut(s) 783
BssNI GRCGYC 2 cut(s) 447, 765
BssSI CACGAG 1 cut(s) 482
BssT1I CCWWGG 1 cut(s) 847
Bst2BI CACGAG 1 cut(s) 482
Bst2UI CCWGG 4 cut(s) 415, 524, 703, 938
Bst4CI ACNGT 6 cut(s) 33, 159, 307, 348, 602, 817
Bst6I CTCTTC 3 cut(s) 110, 131, 291
BstACI GRCGYC 2 cut(s) 447, 765
BstAFI CTTAAG 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 658
BstDSI CCRYGG 1 cut(s) 625
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 1 cut(s) 366
BstFNI CGCG 2 cut(s) 863, 971
BstH2I RGCGCY 3 cut(s) 240, 285, 450
BstHHI GCGC 6 cut(s) 239, 284, 449, 701, 863, 865
BstKTI GATC 1 cut(s) 786
BstMBI GATC 1 cut(s) 783
BstMCI CGRYCG 2 cut(s) 597, 954
BstMWI GCNNNNNNNGC 4 cut(s) 262, 739, 881, 956
BstNI CCWGG 4 cut(s) 415, 524, 703, 938
BstNSI RCATGY 1 cut(s) 1035
BstSFI CTRYAG 1 cut(s) 607
BstSLI GKGCMC 1 cut(s) 689
BstUI CGCG 2 cut(s) 863, 971
BstV1I GCAGC 2 cut(s) 265, 602
BstV2I GAAGAC 1 cut(s) 27
BstXI CCANNNNNNTGG 3 cut(s) 589, 805, 1011
BsuI GTATCC 2 cut(s) 183, 933
BsuRI GGCC 9 cut(s) 488, 530, 693, 706, 729, 853, 857, 875, 911
BtgI CCRYGG 1 cut(s) 625
BtgZI GCGATG 1 cut(s) 778
BtrI CACGTC 1 cut(s) 589
BtsCI GGATG 1 cut(s) 366
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 5 cut(s) 29, 192, 257, 589, 689
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 6 cut(s) 239, 284, 449, 701, 863, 865
Cfr10I RCCGGY 3 cut(s) 536, 853, 890
Cfr9I CCCGGG 1 cut(s) 917
CsiI ACCWGGT 1 cut(s) 522
Csp6I GTAC 5 cut(s) 271, 322, 436, 829, 841
CviAII CATG 3 cut(s) 781, 1009, 1032
CviQI GTAC 5 cut(s) 271, 322, 436, 829, 841
DinI GGCGCC 1 cut(s) 448
DpnI GATC 1 cut(s) 785
DpnII GATC 1 cut(s) 783
DraIII CACNNNGTG 1 cut(s) 526
DrdI GACNNNNNNGTC 1 cut(s) 593
DseDI GACNNNNNNGTC 1 cut(s) 593
EaeI YGGCCR 2 cut(s) 691, 855
Eam1104I CTCTTC 3 cut(s) 110, 131, 291
EarI CTCTTC 3 cut(s) 110, 131, 291
EciI GGCGGA 2 cut(s) 394, 729
Eco130I CCWWGG 1 cut(s) 847
Eco147I AGGCCT 1 cut(s) 488
Eco47I GGWCC 5 cut(s) 348, 621, 679, 934, 1015
Eco88I CYCGRG 2 cut(s) 869, 917
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoO109I RGGNCCY 4 cut(s) 621, 909, 934, 1015
EcoRII CCWGG 4 cut(s) 413, 522, 701, 936
EcoT14I CCWWGG 1 cut(s) 847
EgeI GGCGCC 1 cut(s) 448
EheI GGCGCC 1 cut(s) 448
ErhI CCWWGG 1 cut(s) 847
FaeI CATG 3 cut(s) 784, 1012, 1035
FaiI YATR 6 cut(s) 54, 782, 788, 966, 1010, 1033
FaqI GGGAC 4 cut(s) 490, 634, 653, 1028
FatI CATG 3 cut(s) 780, 1008, 1031
FauI CCCGC 4 cut(s) 611, 738, 802, 1026
FbaI TGATCA 1 cut(s) 783
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 6 cut(s) 254, 445, 613, 616, 866, 1040
FokI GGATG 1 cut(s) 353
FseI GGCCGGCC 1 cut(s) 857
Fsp4HI GCNGC 6 cut(s) 254, 445, 613, 616, 866, 1040
GlaI GCGC 6 cut(s) 238, 283, 448, 700, 862, 864
GluI GCNGC 6 cut(s) 254, 445, 613, 616, 866, 1040
HaeII RGCGCY 3 cut(s) 240, 285, 450
HaeIII GGCC 9 cut(s) 488, 530, 693, 706, 729, 853, 857, 875, 911
HhaI GCGC 6 cut(s) 239, 284, 449, 701, 863, 865
Hin1I GRCGYC 2 cut(s) 447, 765
Hin1II CATG 3 cut(s) 784, 1012, 1035
Hin6I GCGC 6 cut(s) 237, 282, 447, 699, 861, 863
HinP1I GCGC 6 cut(s) 237, 282, 447, 699, 861, 863
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 1 cut(s) 330
HphI GGTGA 5 cut(s) 69, 147, 295, 325, 704
Hpy166II GTNNAC 2 cut(s) 598, 982
Hpy188I TCNGA 6 cut(s) 167, 177, 500, 756, 770, 902
Hpy188III TCNNGA 3 cut(s) 48, 133, 667
Hpy8I GTNNAC 2 cut(s) 598, 982
Hpy99I CGWCG 5 cut(s) 464, 467, 602, 767, 770
HpyAV CCTTC 2 cut(s) 130, 172
HpyCH4III ACNGT 6 cut(s) 33, 159, 307, 348, 602, 817
HpyCH4IV ACGT 4 cut(s) 567, 588, 657, 765
HpyCH4V TGCA 3 cut(s) 888, 1031, 1035
HpyF10VI GCNNNNNNNGC 4 cut(s) 262, 739, 881, 956
HpySE526I ACGT 4 cut(s) 567, 588, 657, 765
Hsp92I GRCGYC 2 cut(s) 447, 765
Hsp92II CATG 3 cut(s) 784, 1012, 1035
HspAI GCGC 6 cut(s) 237, 282, 447, 699, 861, 863
KasI GGCGCC 1 cut(s) 446
KflI GGGWCCC 2 cut(s) 621, 1015
Kpn2I TCCGGA 1 cut(s) 47
KroI GCCGGC 2 cut(s) 536, 853
KroNI GCCGGC 2 cut(s) 538, 855
Ksp22I TGATCA 1 cut(s) 783
Kzo9I GATC 1 cut(s) 783
LguI GCTCTTC 1 cut(s) 291
LmnI GCTCC 4 cut(s) 210, 250, 635, 757
Lsp1109I GCAGC 2 cut(s) 265, 602
MabI ACCWGGT 1 cut(s) 522
MaeII ACGT 4 cut(s) 567, 588, 657, 765
MaeIII GTNAC 3 cut(s) 512, 584, 817
MalI GATC 1 cut(s) 785
MbiI CCGCTC 1 cut(s) 868
MboI GATC 1 cut(s) 783
MboII GAAGA 4 cut(s) 27, 97, 148, 278
MhlI GDGCHC 2 cut(s) 267, 689
MluCI AATT 1 cut(s) 73
Mly113I GGCGCC 1 cut(s) 447
MmeI TCCRAC 3 cut(s) 367, 657, 748
MroI TCCGGA 1 cut(s) 47
MroNI GCCGGC 2 cut(s) 536, 853
MseI TTAA 2 cut(s) 147, 291
MspA1I CMGCKG 1 cut(s) 615
MspCI CTTAAG 1 cut(s) 290
MvaI CCWGG 4 cut(s) 415, 524, 703, 938
MvnI CGCG 2 cut(s) 863, 971
MwoI GCNNNNNNNGC 4 cut(s) 262, 739, 881, 956
NaeI GCCGGC 2 cut(s) 538, 855
NarI GGCGCC 1 cut(s) 447
NciI CCSGG 9 cut(s) 228, 385, 638, 690, 907, 918, 919, 927, 955
NdeII GATC 1 cut(s) 783
NgoMIV GCCGGC 2 cut(s) 536, 853
NlaIII CATG 3 cut(s) 784, 1012, 1035
NmuCI GTSAC 1 cut(s) 584
NspI RCATGY 1 cut(s) 1035
PaeI GCATGC 1 cut(s) 1035
PaeR7I CTCGAG 1 cut(s) 869
PauI GCGCGC 1 cut(s) 861
PceI AGGCCT 1 cut(s) 488
PciSI GCTCTTC 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 594
PdiI GCCGGC 2 cut(s) 538, 855
PfeI GAWTC 1 cut(s) 330
PkrI GCNGC 6 cut(s) 255, 446, 614, 617, 867, 1041
PluTI GGCGCC 1 cut(s) 450
Ppu21I YACGTR 1 cut(s) 658
PpuMI RGGWCCY 3 cut(s) 621, 934, 1015
Psp1406I AACGTT 1 cut(s) 567
Psp5II RGGWCCY 3 cut(s) 621, 934, 1015
Psp6I CCWGG 4 cut(s) 413, 522, 701, 936
PspGI CCWGG 4 cut(s) 413, 522, 701, 936
PspPPI RGGWCCY 3 cut(s) 621, 934, 1015
PspXI VCTCGAGB 1 cut(s) 869
PstNI CAGNNNCTG 1 cut(s) 259
PteI GCGCGC 1 cut(s) 861
RigI GGCCGGCC 1 cut(s) 857
RsaI GTAC 5 cut(s) 272, 323, 437, 830, 842
RsaNI GTAC 5 cut(s) 271, 322, 436, 829, 841
SalI GTCGAC 1 cut(s) 596
SapI GCTCTTC 1 cut(s) 291
SaqAI TTAA 2 cut(s) 147, 291
SatI GCNGC 6 cut(s) 254, 445, 613, 616, 866, 1040
Sau3AI GATC 1 cut(s) 783
SduI GDGCHC 2 cut(s) 267, 689
SetI ASST 8 cut(s) 525, 570, 591, 660, 768, 965, 995, 1028
SexAI ACCWGGT 1 cut(s) 522
SfcI CTRYAG 1 cut(s) 607
SfoI GGCGCC 1 cut(s) 448
Sfr274I CTCGAG 1 cut(s) 869
SgrAI CRCCGGYG 1 cut(s) 890
SinI GGWCC 5 cut(s) 348, 621, 679, 934, 1015
SlaI CTCGAG 1 cut(s) 869
SmaI CCCGGG 1 cut(s) 919
SmlI CTYRAG 3 cut(s) 266, 290, 869
SmoI CTYRAG 3 cut(s) 266, 290, 869
SphI GCATGC 1 cut(s) 1035
Sse9I AATT 1 cut(s) 73
SseBI AGGCCT 1 cut(s) 488
SspDI GGCGCC 1 cut(s) 446
StuI AGGCCT 1 cut(s) 488
StyI CCWWGG 1 cut(s) 847
TaaI ACNGT 6 cut(s) 33, 159, 307, 348, 602, 817
TaiI ACGT 4 cut(s) 570, 591, 660, 768
TaqI TCGA 3 cut(s) 360, 597, 870
TasI AATT 1 cut(s) 73
TatI WGTACW 1 cut(s) 435
TauI GCSGC 4 cut(s) 447, 615, 868, 1042
TfiI GAWTC 1 cut(s) 330
Tru1I TTAA 2 cut(s) 147, 291
Tru9I TTAA 2 cut(s) 147, 291
TscAI CASTG 5 cut(s) 36, 199, 264, 589, 689
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 2 cut(s) 253, 615
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 2 cut(s) 17, 355
TspGWI ACGGA 2 cut(s) 500, 816
TspMI CCCGGG 1 cut(s) 917
TspRI CASTG 5 cut(s) 36, 199, 264, 589, 689
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 5 cut(s) 348, 621, 679, 934, 1015
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 1 cut(s) 73
XceI RCATGY 1 cut(s) 1035
XhoI CTCGAG 1 cut(s) 869
XmaI CCCGGG 1 cut(s) 917
XmiI GTMKAC 1 cut(s) 597
ZraI GACGTC 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.