Rh6AG180400

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002144
Physical Location & Seq
Reverse (-)
31464474 .. 31466530
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG180400.1

Sequence Viewer

Length: 912 bp
ATGTCTTGTATTCACCACTTTGCAATTGCTTTCTTCCTCTTGTTTCTGGTCCAGCCCGGTTTGGGTGGTCGGAAACTCTCCGCTCTAGTGGAGCAGCAGCCACTGGTGCTCAAGTACCACAACGGCGCCCTTCTCAAGGGCAACATCACCGTCAATCTCATATGGTACGGCCAATTTACCCCAATCCAGCGCTCCATAATCGTCGACTTCATCCAGTCCCTCAGTTCCCGGCATCGTGCCCCACTCCTTCCCTCAGCCTCTTCCTGGTGGAAAACCACCGAGAAATACAAGACCGGGTCCTCCAATCTGGTCGTGGGGAGACAAGTGCTCCGCGAGGCCTACACTCTCGGAAAGTCCCTGACGAGCTCACACCTGGTCGCTCTTGCCGGGGTCAACAAAATCAACACCCCCTTGAACGCCATCAACGTGGTGCTCACCGCCAAGGACGTGGTAGTTGATGGCTTCTGCAGGAGCCGCTGCGGGACCCACGGATCGTCCCGCTCCGGCAAAGCTGCCTATGTCTGGGTTGGGAACTCCGAGTCGCAGTGTCCGGGCCAATGCGCGTGGCCCTTCCACCAGCCCATGTACGGCCCACAAACTCCTCCTTTGGTGGCGCCAAACGGCGACGTCGGGATAGACGGGATGATAATCAACCTCGCCACGCTCTTGGCGGGAACGGTCACCAACCCCTACAACAACGGCTACTTTCAAGGGCCGCCTACAGCGCCTCTCGAGGCCGTTTCGGCTTGCACCGGCATCTTCGGAACGGGCTCTTACCCGGGTTATCCGGGTCGGGTCCTCGTGGATAAGGCCACCGGGGCGAGCTACAACGCTCACGGGCTGAACGGGCGCAGGTTTCTGTTGCCAGCCATGTGGGACCCTCGGACCTCTGCATGCAAACCACTTGTGTGA

Protein Analysis

303

Amino Acids

32.55

Weight (kDa)

9.59

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 37 - 300 7.3e-121 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 630
Acc36I ACCTGC 1 cut(s) 843
AccB1I GGYRCC 2 cut(s) 125, 613
AccBSI CCGCTC 2 cut(s) 83, 501
AccI GTMKAC 1 cut(s) 204
AccII CGCG 2 cut(s) 333, 563
AciI CCGC 8 cut(s) 81, 331, 438, 475, 480, 499, 671, 716
AclWI GGATC 1 cut(s) 499
AcoI YGGCCR 1 cut(s) 169
AcyI GRCGYC 3 cut(s) 126, 614, 627
AfaI GTAC 3 cut(s) 116, 167, 587
AfeI AGCGCT 1 cut(s) 191
AfiI CCNNNNNNNGG 5 cut(s) 62, 136, 264, 522, 587
AgsI TTSAA 2 cut(s) 415, 710
AjiI CACGTC 1 cut(s) 448
AjnI CCWGG 2 cut(s) 263, 372
AleI CACNNNNGTG 1 cut(s) 907
AluBI AGCT 3 cut(s) 366, 512, 825
AluI AGCT 3 cut(s) 366, 512, 825
Alw21I GWGCWC 4 cut(s) 111, 330, 368, 435
Alw26I GTCTC 1 cut(s) 313
AlwI GGATC 1 cut(s) 499
AlwNI CAGNNNCTG 1 cut(s) 103
Ama87I CYCGRG 2 cut(s) 731, 778
Aor51HI AGCGCT 1 cut(s) 191
AoxI GGCC 8 cut(s) 169, 336, 553, 566, 589, 713, 735, 810
ApeKI GCWGC 4 cut(s) 94, 97, 477, 512
AspLEI GCGC 6 cut(s) 128, 192, 563, 616, 727, 852
AsuC2I CCSGG 9 cut(s) 57, 229, 295, 388, 552, 779, 780, 789, 817
AsuHPI GGTGA 4 cut(s) 5, 139, 427, 673
AvaI CYCGRG 2 cut(s) 731, 778
AvaII GGWCC 6 cut(s) 49, 297, 483, 796, 877, 885
BaeGI GKGCMC 1 cut(s) 241
BanI GGYRCC 2 cut(s) 125, 613
BanII GRGCYC 2 cut(s) 368, 773
BauI CACGAG 1 cut(s) 800
Bbv12I GWGCWC 4 cut(s) 111, 330, 368, 435
BbvCI CCTCAGC 1 cut(s) 253
BbvI GCAGC 4 cut(s) 106, 109, 464, 499
BccI CCATC 2 cut(s) 428, 452
BceAI ACGGC 6 cut(s) 139, 184, 604, 637, 715, 722
BciT130I CCWGG 2 cut(s) 265, 374
BcnI CCSGG 9 cut(s) 57, 229, 295, 388, 552, 779, 780, 789, 817
BcoDI GTCTC 1 cut(s) 313
BfaI CTAG 1 cut(s) 86
BfmI CTRYAG 2 cut(s) 466, 720
BfoI RGCGCY 4 cut(s) 129, 193, 617, 728
BfuAI ACCTGC 1 cut(s) 843
BglI GCCNNNNNGGC 2 cut(s) 743, 818
BisI GCNGC 6 cut(s) 95, 98, 475, 478, 513, 716
BlsI GCNGC 6 cut(s) 96, 99, 476, 479, 514, 717
Bme18I GGWCC 6 cut(s) 49, 297, 483, 796, 877, 885
BmeT110I CYCGRG 2 cut(s) 731, 778
BmgBI CACGTC 1 cut(s) 448
BmiI GGNNCC 9 cut(s) 127, 298, 473, 484, 485, 615, 797, 878, 879
BmsI GCATC 2 cut(s) 241, 765
Bpu10I CCTNAGC 1 cut(s) 253
BpuEI CTTGAG 2 cut(s) 95, 119
BpuMI CCSGG 9 cut(s) 57, 229, 295, 388, 552, 779, 780, 789, 817
BsaBI GATNNNNATC 1 cut(s) 647
BsaHI GRCGYC 3 cut(s) 126, 614, 627
BsaJI CCNNGG 6 cut(s) 387, 441, 487, 778, 816, 881
BsaXI ACNNNNNCTCC 2 cut(s) 312, 342
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 136, 264, 522, 587
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 2 cut(s) 108, 214
Bse8I GATNNNNATC 1 cut(s) 647
BseBI CCWGG 2 cut(s) 265, 374
BseDI CCNNGG 6 cut(s) 387, 441, 487, 778, 816, 881
BseGI GGATG 2 cut(s) 210, 648
BseJI GATNNNNATC 1 cut(s) 647
BseLI CCNNNNNNNGG 5 cut(s) 62, 136, 264, 522, 587
BseMII CTCAG 2 cut(s) 235, 267
BseNI ACTGG 2 cut(s) 108, 214
BseRI GAGGAG 1 cut(s) 591
BseSI GKGCMC 1 cut(s) 241
BseXI GCAGC 4 cut(s) 106, 109, 464, 499
Bsh1236I CGCG 2 cut(s) 333, 563
BshFI GGCC 8 cut(s) 171, 338, 555, 568, 591, 715, 737, 812
BshNI GGYRCC 2 cut(s) 125, 613
BsiHKAI GWGCWC 4 cut(s) 111, 330, 368, 435
BsiHKCI CYCGRG 2 cut(s) 731, 778
BslFI GGGAC 5 cut(s) 202, 340, 481, 496, 890
BslI CCNNNNNNNGG 5 cut(s) 62, 136, 264, 522, 587
BsmAI GTCTC 1 cut(s) 313
BsmFI GGGAC 5 cut(s) 202, 340, 481, 496, 890
BsnI GGCC 8 cut(s) 171, 338, 555, 568, 591, 715, 737, 812
BsoBI CYCGRG 2 cut(s) 731, 778
Bsp1286I GDGCHC 6 cut(s) 111, 241, 330, 368, 435, 773
Bsp143I GATC 1 cut(s) 491
BspACI CCGC 8 cut(s) 81, 331, 438, 475, 480, 499, 671, 716
BspANI GGCC 8 cut(s) 171, 338, 555, 568, 591, 715, 737, 812
BspCNI CTCAG 2 cut(s) 234, 266
BspFNI CGCG 2 cut(s) 333, 563
BspLI GGNNCC 9 cut(s) 127, 298, 473, 484, 485, 615, 797, 878, 879
BspMAI CTGCAG 1 cut(s) 470
BspMI ACCTGC 1 cut(s) 843
BspPI GGATC 1 cut(s) 499
BspT107I GGYRCC 2 cut(s) 125, 613
BsrBI CCGCTC 2 cut(s) 83, 501
BsrFI RCCGGY 1 cut(s) 752
BsrI ACTGG 2 cut(s) 108, 214
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 6 cut(s) 387, 441, 487, 778, 816, 881
BssMI GATC 1 cut(s) 491
BssNI GRCGYC 3 cut(s) 126, 614, 627
BssSI CACGAG 1 cut(s) 800
BssT1I CCWWGG 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 800
Bst2UI CCWGG 2 cut(s) 265, 374
Bst4CI ACNGT 2 cut(s) 151, 679
Bst6I CTCTTC 1 cut(s) 265
BstACI GRCGYC 3 cut(s) 126, 614, 627
BstC8I GCNNGC 4 cut(s) 748, 823, 867, 895
BstDEI CTNAG 2 cut(s) 221, 253
BstDSI CCRYGG 1 cut(s) 487
BstEII GGTNACC 1 cut(s) 679
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 2 cut(s) 210, 648
BstFNI CGCG 2 cut(s) 333, 563
BstH2I RGCGCY 4 cut(s) 129, 193, 617, 728
BstHHI GCGC 6 cut(s) 128, 192, 563, 616, 727, 852
BstKTI GATC 1 cut(s) 494
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 1 cut(s) 491
BstMWI GCNNNNNNNGC 6 cut(s) 106, 474, 724, 743, 818, 847
BstNI CCWGG 2 cut(s) 265, 374
BstNSI RCATGY 1 cut(s) 897
BstPI GGTNACC 1 cut(s) 679
BstSFI CTRYAG 2 cut(s) 466, 720
BstSLI GKGCMC 1 cut(s) 241
BstUI CGCG 2 cut(s) 333, 563
BstV1I GCAGC 4 cut(s) 106, 109, 464, 499
BstXI CCANNNNNNTGG 4 cut(s) 427, 448, 667, 873
BsuRI GGCC 8 cut(s) 171, 338, 555, 568, 591, 715, 737, 812
BtgI CCRYGG 1 cut(s) 487
BtrI CACGTC 1 cut(s) 448
BtsCI GGATG 2 cut(s) 210, 648
BtsI GCAGTG 1 cut(s) 551
BtsIMutI CAGTG 2 cut(s) 101, 551
BveI ACCTGC 1 cut(s) 843
Cac8I GCNNGC 4 cut(s) 748, 823, 867, 895
CaiI CAGNNNCTG 1 cut(s) 103
CfoI GCGC 6 cut(s) 128, 192, 563, 616, 727, 852
Cfr10I RCCGGY 1 cut(s) 752
Cfr9I CCCGGG 1 cut(s) 778
CsiI ACCWGGT 1 cut(s) 372
Csp6I GTAC 3 cut(s) 115, 166, 586
CspCI CAANNNNNGTGG 2 cut(s) 545, 580
CviAII CATG 3 cut(s) 583, 871, 894
CviQI GTAC 3 cut(s) 115, 166, 586
DdeI CTNAG 2 cut(s) 221, 253
DinI GGCGCC 2 cut(s) 127, 615
DpnI GATC 1 cut(s) 493
DpnII GATC 1 cut(s) 491
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Ecl136II GAGCTC 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 441
Eco147I AGGCCT 1 cut(s) 338
Eco24I GRGCYC 2 cut(s) 368, 773
Eco47I GGWCC 6 cut(s) 49, 297, 483, 796, 877, 885
Eco47III AGCGCT 1 cut(s) 191
Eco53kI GAGCTC 1 cut(s) 366
Eco88I CYCGRG 2 cut(s) 731, 778
Eco91I GGTNACC 1 cut(s) 679
EcoICRI GAGCTC 1 cut(s) 366
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoO109I RGGNCCY 4 cut(s) 297, 483, 796, 877
EcoO65I GGTNACC 1 cut(s) 679
EcoRII CCWGG 2 cut(s) 263, 372
EcoT14I CCWWGG 1 cut(s) 441
EcoT38I GRGCYC 2 cut(s) 368, 773
EgeI GGCGCC 2 cut(s) 127, 615
EheI GGCGCC 2 cut(s) 127, 615
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 3 cut(s) 586, 874, 897
FaiI YATR 7 cut(s) 161, 163, 197, 519, 584, 872, 895
FaqI GGGAC 5 cut(s) 202, 340, 481, 496, 890
FatI CATG 3 cut(s) 582, 870, 893
FauI CCCGC 3 cut(s) 473, 506, 664
FauNDI CATATG 1 cut(s) 161
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 6 cut(s) 95, 98, 475, 478, 513, 716
FokI GGATG 2 cut(s) 197, 655
FriOI GRGCYC 2 cut(s) 368, 773
Fsp4HI GCNGC 6 cut(s) 95, 98, 475, 478, 513, 716
FspBI CTAG 1 cut(s) 86
GlaI GCGC 6 cut(s) 127, 191, 562, 615, 726, 851
GluI GCNGC 6 cut(s) 95, 98, 475, 478, 513, 716
HaeII RGCGCY 4 cut(s) 129, 193, 617, 728
HaeIII GGCC 8 cut(s) 171, 338, 555, 568, 591, 715, 737, 812
HhaI GCGC 6 cut(s) 128, 192, 563, 616, 727, 852
Hin1I GRCGYC 3 cut(s) 126, 614, 627
Hin1II CATG 3 cut(s) 586, 874, 897
Hin6I GCGC 6 cut(s) 126, 190, 561, 614, 725, 850
HinP1I GCGC 6 cut(s) 126, 190, 561, 614, 725, 850
HincII GTYRAC 2 cut(s) 205, 394
HindII GTYRAC 2 cut(s) 205, 394
HinfI GANTC 1 cut(s) 539
HphI GGTGA 4 cut(s) 5, 139, 427, 673
Hpy166II GTNNAC 2 cut(s) 205, 394
Hpy188I TCNGA 5 cut(s) 72, 350, 538, 764, 885
Hpy188III TCNNGA 2 cut(s) 631, 731
Hpy8I GTNNAC 2 cut(s) 205, 394
Hpy99I CGWCG 3 cut(s) 206, 629, 632
HpyAV CCTTC 3 cut(s) 140, 257, 580
HpyCH4III ACNGT 2 cut(s) 151, 679
HpyCH4IV ACGT 3 cut(s) 426, 447, 627
HpyCH4V TGCA 5 cut(s) 23, 468, 750, 893, 897
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 474, 724, 743, 818, 847
HpyF3I CTNAG 2 cut(s) 221, 253
HpySE526I ACGT 3 cut(s) 426, 447, 627
Hsp92I GRCGYC 3 cut(s) 126, 614, 627
Hsp92II CATG 3 cut(s) 586, 874, 897
HspAI GCGC 6 cut(s) 126, 190, 561, 614, 725, 850
KasI GGCGCC 2 cut(s) 125, 613
KflI GGGWCCC 2 cut(s) 483, 877
Kzo9I GATC 1 cut(s) 491
LmnI GCTCC 5 cut(s) 91, 197, 333, 471, 506
Lsp1109I GCAGC 4 cut(s) 106, 109, 464, 499
LweI GCATC 2 cut(s) 241, 765
MabI ACCWGGT 1 cut(s) 372
MaeI CTAG 1 cut(s) 86
MaeII ACGT 3 cut(s) 426, 447, 627
MaeIII GTNAC 1 cut(s) 679
MalI GATC 1 cut(s) 493
MbiI CCGCTC 2 cut(s) 83, 501
MboI GATC 1 cut(s) 491
MboII GAAGA 3 cut(s) 25, 252, 751
MfeI CAATTG 1 cut(s) 24
MhlI GDGCHC 6 cut(s) 111, 241, 330, 368, 435, 773
MluCI AATT 2 cut(s) 24, 173
Mly113I GGCGCC 2 cut(s) 126, 614
MlyI GAGTC 1 cut(s) 548
MmeI TCCRAC 1 cut(s) 50
MslI CAYNNNNRTG 2 cut(s) 425, 907
MspA1I CMGCKG 1 cut(s) 477
MunI CAATTG 1 cut(s) 24
MvaI CCWGG 2 cut(s) 265, 374
MvnI CGCG 2 cut(s) 333, 563
MwoI GCNNNNNNNGC 6 cut(s) 106, 474, 724, 743, 818, 847
NarI GGCGCC 2 cut(s) 126, 614
NciI CCSGG 9 cut(s) 57, 229, 295, 388, 552, 779, 780, 789, 817
NdeI CATATG 1 cut(s) 161
NdeII GATC 1 cut(s) 491
NlaIII CATG 3 cut(s) 586, 874, 897
NlaIV GGNNCC 9 cut(s) 127, 298, 473, 484, 485, 615, 797, 878, 879
NmuCI GTSAC 1 cut(s) 679
NspI RCATGY 1 cut(s) 897
OliI CACNNNNGTG 1 cut(s) 907
PaeI GCATGC 1 cut(s) 897
PaeR7I CTCGAG 1 cut(s) 731
PceI AGGCCT 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PflFI GACNNNGTC 1 cut(s) 295
PkrI GCNGC 6 cut(s) 96, 99, 476, 479, 514, 717
PleI GAGTC 1 cut(s) 547
PluTI GGCGCC 2 cut(s) 129, 617
PpsI GAGTC 1 cut(s) 547
PpuMI RGGWCCY 4 cut(s) 297, 483, 796, 877
Psp124BI GAGCTC 1 cut(s) 368
Psp5II RGGWCCY 4 cut(s) 297, 483, 796, 877
Psp6I CCWGG 2 cut(s) 263, 372
PspEI GGTNACC 1 cut(s) 679
PspGI CCWGG 2 cut(s) 263, 372
PspN4I GGNNCC 9 cut(s) 127, 298, 473, 484, 485, 615, 797, 878, 879
PspPPI RGGWCCY 4 cut(s) 297, 483, 796, 877
PstI CTGCAG 1 cut(s) 470
PstNI CAGNNNCTG 1 cut(s) 103
PsyI GACNNNGTC 1 cut(s) 295
RsaI GTAC 3 cut(s) 116, 167, 587
RsaNI GTAC 3 cut(s) 115, 166, 586
RseI CAYNNNNRTG 2 cut(s) 425, 907
SacI GAGCTC 1 cut(s) 368
SalI GTCGAC 1 cut(s) 203
SatI GCNGC 6 cut(s) 95, 98, 475, 478, 513, 716
Sau3AI GATC 1 cut(s) 491
SchI GAGTC 1 cut(s) 548
SduI GDGCHC 6 cut(s) 111, 241, 330, 368, 435, 773
SexAI ACCWGGT 1 cut(s) 372
SfaNI GCATC 2 cut(s) 241, 765
SfcI CTRYAG 2 cut(s) 466, 720
SfoI GGCGCC 2 cut(s) 127, 615
Sfr274I CTCGAG 1 cut(s) 731
SinI GGWCC 6 cut(s) 49, 297, 483, 796, 877, 885
SlaI CTCGAG 1 cut(s) 731
SmaI CCCGGG 1 cut(s) 780
SmiMI CAYNNNNRTG 2 cut(s) 425, 907
SmlI CTYRAG 3 cut(s) 110, 134, 731
SmoI CTYRAG 3 cut(s) 110, 134, 731
SphI GCATGC 1 cut(s) 897
Sse9I AATT 2 cut(s) 24, 173
SseBI AGGCCT 1 cut(s) 338
SsiI CCGC 8 cut(s) 81, 331, 438, 475, 480, 499, 671, 716
SspDI GGCGCC 2 cut(s) 125, 613
SspMI CTAG 1 cut(s) 86
SstI GAGCTC 1 cut(s) 368
StuI AGGCCT 1 cut(s) 338
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 2 cut(s) 151, 679
TaiI ACGT 3 cut(s) 429, 450, 630
TaqI TCGA 2 cut(s) 204, 732
TasI AATT 2 cut(s) 24, 173
TauI GCSGC 2 cut(s) 477, 718
TscAI CASTG 2 cut(s) 108, 551
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 4 cut(s) 94, 97, 477, 512
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 1 cut(s) 199
TspGWI ACGGA 1 cut(s) 504
TspMI CCCGGG 1 cut(s) 778
TspRI CASTG 2 cut(s) 108, 551
Tth111I GACNNNGTC 1 cut(s) 295
VpaK11BI GGWCC 6 cut(s) 49, 297, 483, 796, 877, 885
XagI CCTNNNNNAGG 1 cut(s) 134
XceI RCATGY 1 cut(s) 897
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XhoI CTCGAG 1 cut(s) 731
XmaI CCCGGG 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 86
ZraI GACGTC 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.