MD08G1186400.v1.1

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002144
Physical Location & Seq
Forward (+)
23454169 .. 23455101
933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1186400.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGCTTTTACTTCCCATTTGGCCATCTGGGTCACATTTTTTCTTACCATTTCTTCCTTCCTCAGCCCCTGTACAGCCACCATGCGCAAGCTCTCAGCTTTAGTGCAGCAACAGCCGCTTGTGCTCAATTACCACAATGGTCCACTCCTTAAGGGGAACCTCACCGTCAATATCGTATGGTACGGCAAGTTCTCCCCCAGCCAACGGTCCATACTAGTCGACTTTGTTCAGTCTCTGAGCTCCGGACAGCGCACCCCGCAGCCCTCCGTCTCGTCGTGGTGGCAGACTACTGGAGGATACAAAGGAGGCCCCTCTACCCCCATCGTAGGGAATCAAATCCTGGACGAAACTTACTCTCTCGGAAAAGCACTCACAGATCAGAAGCTCGTAGCTCTGGCAGCCAAAGCATCAAAAGGCCAAAACGCAGCGATTAACGTCGTTTTGACCGGTGCCGATGTGACAGTTGACGGGTTCTGTAGCAGCCGCTGCGGCTCCCACGGATCGGGCATTGAGGGGAGAAAGACGGGCAAGCTGGCTTACGCTTGGGTGGGTAACCCGGCGAGCCAGGCTCCGGGTCAGTGCGCATGGCCATTTCACCAGCCAATTTACGGGCCGCAGACGCCTCCGTTAGTTCCTCCAAACGGCGATGTGGGGATCGACGGAATGGTTATCAGTTTGGCTACGGTTTTGGCGGGAGCCGTGACAAACCCGTTTGACAACGGATATTTTCAGGGATCGAAAGACGCGCCCTTGGAGGCGGTGAGCGCGTGCACTGGTATATTTGGGAAAGGGGCTTACCCGGGTTATCCTGGAGACGTTATTCTGGACAAAACGACGGGAGTTAGCTACAATGCGTTTGGGGTACGTGGACGCAAATATTTGCTCCCGGCGATGTGGGACCCACAGACCTCCGCGTGCAAAACACTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

311

Amino Acids

32.74

Weight (kDa)

9.07

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 42 - 309 4.8e-119 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 86, 583
AccB1I GGYRCC 1 cut(s) 449
AccI GTMKAC 1 cut(s) 219
AccII CGCG 3 cut(s) 746, 767, 914
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 8 cut(s) 116, 257, 484, 489, 614, 692, 758, 912
AclWI GGATC 3 cut(s) 508, 662, 742
AcoI YGGCCR 2 cut(s) 21, 587
AcyI GRCGYC 1 cut(s) 620
AfaI GTAC 3 cut(s) 73, 182, 864
AfiI CCNNNNNNNGG 7 cut(s) 204, 326, 327, 502, 571, 608, 641
AflII CTTAAG 1 cut(s) 149
AgeI ACCGGT 1 cut(s) 446
AhlI ACTAGT 1 cut(s) 214
AjnI CCWGG 3 cut(s) 339, 564, 808
AluBI AGCT 7 cut(s) 91, 98, 240, 385, 392, 532, 846
AluI AGCT 7 cut(s) 91, 98, 240, 385, 392, 532, 846
Alw21I GWGCWC 3 cut(s) 126, 242, 773
Alw26I GTCTC 3 cut(s) 237, 274, 807
Alw44I GTGCAC 1 cut(s) 769
AlwI GGATC 3 cut(s) 508, 662, 742
AlwNI CAGNNNCTG 3 cut(s) 69, 235, 486
Ama87I CYCGRG 1 cut(s) 799
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 5 cut(s) 21, 307, 415, 587, 611
ApaLI GTGCAC 1 cut(s) 769
ApeKI GCWGC 6 cut(s) 106, 259, 398, 425, 480, 486
AsiGI ACCGGT 1 cut(s) 446
AspLEI GCGC 5 cut(s) 87, 252, 584, 748, 767
AspS9I GGNCC 5 cut(s) 140, 207, 308, 611, 898
AsuC2I CCSGG 5 cut(s) 557, 573, 800, 801, 887
AsuHPI GGTGA 3 cut(s) 154, 587, 772
AvaI CYCGRG 1 cut(s) 799
AvaII GGWCC 3 cut(s) 140, 207, 898
BaeGI GKGCMC 1 cut(s) 773
BalI TGGCCA 2 cut(s) 23, 589
BanI GGYRCC 1 cut(s) 449
BanII GRGCYC 1 cut(s) 242
Bbv12I GWGCWC 3 cut(s) 126, 242, 773
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 6 cut(s) 118, 271, 410, 437, 473, 492
BccI CCATC 2 cut(s) 32, 329
BceAI ACGGC 3 cut(s) 199, 658, 683
BcgI CGANNNNNNTGC 1 cut(s) 907
BciT130I CCWGG 3 cut(s) 341, 566, 810
BciVI GTATCC 1 cut(s) 290
BcnI CCSGG 5 cut(s) 557, 573, 800, 801, 887
BcoDI GTCTC 3 cut(s) 237, 274, 807
BcuI ACTAGT 1 cut(s) 214
BfaI CTAG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 475
BfrI CTTAAG 1 cut(s) 149
BfuI GTATCC 1 cut(s) 290
BglI GCCNNNNNGGC 1 cut(s) 489
Bme1390I CCNGG 8 cut(s) 341, 557, 566, 573, 800, 801, 810, 887
Bme18I GGWCC 3 cut(s) 140, 207, 898
BmeT110I CYCGRG 1 cut(s) 799
BmgT120I GGNCC 5 cut(s) 140, 207, 308, 611, 898
BmiI GGNNCC 8 cut(s) 158, 310, 451, 493, 570, 697, 899, 900
BmrFI CCNGG 8 cut(s) 341, 557, 566, 573, 800, 801, 810, 887
BmsI GCATC 1 cut(s) 416
BplI GAGNNNNNCTC 2 cut(s) 553, 585
BpmI CTGGAG 2 cut(s) 312, 831
Bpu10I CCTNAGC 1 cut(s) 62
BpuMI CCSGG 5 cut(s) 557, 573, 800, 801, 887
BsaAI YACGTR 1 cut(s) 866
BsaHI GRCGYC 1 cut(s) 620
BsaJI CCNNGG 3 cut(s) 496, 750, 799
BsaWI WCCGGW 2 cut(s) 242, 446
Bsc4I CCNNNNNNNGG 7 cut(s) 204, 326, 327, 502, 571, 608, 641
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 2 cut(s) 295, 778
BseAI TCCGGA 1 cut(s) 242
BseBI CCWGG 3 cut(s) 341, 566, 810
BseDI CCNNGG 3 cut(s) 496, 750, 799
BseLI CCNNNNNNNGG 7 cut(s) 204, 326, 327, 502, 571, 608, 641
BseMII CTCAG 3 cut(s) 76, 108, 227
BseNI ACTGG 2 cut(s) 295, 778
BseSI GKGCMC 1 cut(s) 773
BseXI GCAGC 6 cut(s) 118, 271, 410, 437, 473, 492
BseYI CCCAGC 1 cut(s) 197
BsgI GTGCAG 1 cut(s) 125
Bsh1236I CGCG 3 cut(s) 746, 767, 914
BshFI GGCC 5 cut(s) 23, 309, 417, 589, 613
BshNI GGYRCC 1 cut(s) 449
BshTI ACCGGT 1 cut(s) 446
BsiHKAI GWGCWC 3 cut(s) 126, 242, 773
BsiHKCI CYCGRG 1 cut(s) 799
BsiSI CCGG 6 cut(s) 243, 447, 557, 572, 800, 887
BslFI GGGAC 1 cut(s) 911
BslI CCNNNNNNNGG 7 cut(s) 204, 326, 327, 502, 571, 608, 641
BsmAI GTCTC 3 cut(s) 237, 274, 807
BsmBI CGTCTC 2 cut(s) 274, 807
BsmFI GGGAC 1 cut(s) 911
BsnI GGCC 5 cut(s) 23, 309, 417, 589, 613
BsoBI CYCGRG 1 cut(s) 799
Bsp1286I GDGCHC 3 cut(s) 126, 242, 773
Bsp13I TCCGGA 1 cut(s) 242
Bsp1407I TGTACA 1 cut(s) 71
Bsp143I GATC 4 cut(s) 376, 500, 654, 734
BspACI CCGC 8 cut(s) 116, 257, 484, 489, 614, 692, 758, 912
BspANI GGCC 5 cut(s) 23, 309, 417, 589, 613
BspCNI CTCAG 3 cut(s) 75, 107, 228
BspEI TCCGGA 1 cut(s) 242
BspFNI CGCG 3 cut(s) 746, 767, 914
BspLI GGNNCC 8 cut(s) 158, 310, 451, 493, 570, 697, 899, 900
BspPI GGATC 3 cut(s) 508, 662, 742
BspT107I GGYRCC 1 cut(s) 449
BspTI CTTAAG 1 cut(s) 149
BsrFI RCCGGY 1 cut(s) 446
BsrGI TGTACA 1 cut(s) 71
BsrI ACTGG 2 cut(s) 295, 778
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 3 cut(s) 496, 750, 799
BssMI GATC 4 cut(s) 376, 500, 654, 734
BssNI GRCGYC 1 cut(s) 620
BssT1I CCWWGG 1 cut(s) 750
Bst2UI CCWGG 3 cut(s) 341, 566, 810
Bst4CI ACNGT 4 cut(s) 166, 207, 463, 685
BstACI GRCGYC 1 cut(s) 620
BstAFI CTTAAG 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 486
BstAUI TGTACA 1 cut(s) 71
BstBAI YACGTR 1 cut(s) 866
BstC8I GCNNGC 6 cut(s) 89, 530, 534, 562, 769, 916
BstDEI CTNAG 3 cut(s) 62, 94, 236
BstDSI CCRYGG 1 cut(s) 496
BstEII GGTNACC 1 cut(s) 551
BstFNI CGCG 3 cut(s) 746, 767, 914
BstHHI GCGC 5 cut(s) 87, 252, 584, 748, 767
BstKTI GATC 4 cut(s) 379, 503, 657, 737
BstMAI GTCTC 3 cut(s) 237, 274, 807
BstMBI GATC 4 cut(s) 376, 500, 654, 734
BstNI CCWGG 3 cut(s) 341, 566, 810
BstPI GGTNACC 1 cut(s) 551
BstSCI CCNGG 8 cut(s) 339, 555, 564, 571, 798, 799, 808, 885
BstSFI CTRYAG 1 cut(s) 475
BstSLI GKGCMC 1 cut(s) 773
BstUI CGCG 3 cut(s) 746, 767, 914
BstV1I GCAGC 6 cut(s) 118, 271, 410, 437, 473, 492
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 5 cut(s) 23, 309, 417, 589, 613
BtgI CCRYGG 1 cut(s) 496
BtgZI GCGATG 2 cut(s) 660, 905
BtsIMutI CAGTG 2 cut(s) 584, 771
Cac8I GCNNGC 6 cut(s) 89, 530, 534, 562, 769, 916
CaiI CAGNNNCTG 3 cut(s) 69, 235, 486
CfoI GCGC 5 cut(s) 87, 252, 584, 748, 767
Cfr10I RCCGGY 1 cut(s) 446
Cfr13I GGNCC 5 cut(s) 140, 207, 308, 611, 898
Cfr9I CCCGGG 1 cut(s) 799
CseI GACGC 3 cut(s) 628, 752, 879
Csp6I GTAC 3 cut(s) 72, 181, 863
CspAI ACCGGT 1 cut(s) 446
CviAII CATG 2 cut(s) 82, 585
CviQI GTAC 3 cut(s) 72, 181, 863
DdeI CTNAG 3 cut(s) 62, 94, 236
DpnI GATC 4 cut(s) 378, 502, 656, 736
DpnII GATC 4 cut(s) 376, 500, 654, 734
EaeI YGGCCR 2 cut(s) 21, 587
Ecl136II GAGCTC 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 750
Eco24I GRGCYC 1 cut(s) 242
Eco47I GGWCC 3 cut(s) 140, 207, 898
Eco53kI GAGCTC 1 cut(s) 240
Eco88I CYCGRG 1 cut(s) 799
Eco91I GGTNACC 1 cut(s) 551
EcoICRI GAGCTC 1 cut(s) 240
EcoO109I RGGNCCY 2 cut(s) 308, 898
EcoO65I GGTNACC 1 cut(s) 551
EcoRII CCWGG 3 cut(s) 339, 564, 808
EcoT14I CCWWGG 1 cut(s) 750
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 750
Esp3I CGTCTC 2 cut(s) 274, 807
FaeI CATG 2 cut(s) 85, 588
FaiI YATR 5 cut(s) 83, 178, 212, 586, 779
FaqI GGGAC 1 cut(s) 911
FatI CATG 2 cut(s) 81, 584
FauI CCCGC 2 cut(s) 264, 685
FblI GTMKAC 1 cut(s) 219
FriOI GRGCYC 1 cut(s) 242
FspAI RTGCGCAY 1 cut(s) 583
FspBI CTAG 1 cut(s) 215
FspI TGCGCA 2 cut(s) 86, 583
GlaI GCGC 5 cut(s) 86, 251, 583, 747, 766
GsaI CCCAGC 1 cut(s) 201
GsuI CTGGAG 2 cut(s) 312, 831
HaeIII GGCC 5 cut(s) 23, 309, 417, 589, 613
HapII CCGG 6 cut(s) 243, 447, 557, 572, 800, 887
HgaI GACGC 3 cut(s) 628, 752, 879
HhaI GCGC 5 cut(s) 87, 252, 584, 748, 767
Hin1I GRCGYC 1 cut(s) 620
Hin1II CATG 2 cut(s) 85, 588
Hin6I GCGC 5 cut(s) 85, 250, 582, 746, 765
HinP1I GCGC 5 cut(s) 85, 250, 582, 746, 765
HincII GTYRAC 2 cut(s) 220, 466
HindII GTYRAC 2 cut(s) 220, 466
HinfI GANTC 1 cut(s) 331
HpaII CCGG 6 cut(s) 243, 447, 557, 572, 800, 887
HphI GGTGA 3 cut(s) 154, 587, 772
Hpy166II GTNNAC 5 cut(s) 143, 220, 466, 771, 869
Hpy188I TCNGA 3 cut(s) 237, 362, 381
Hpy188III TCNNGA 2 cut(s) 243, 824
Hpy8I GTNNAC 5 cut(s) 143, 220, 466, 771, 869
Hpy99I CGWCG 4 cut(s) 277, 440, 662, 838
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 4 cut(s) 166, 207, 463, 685
HpyCH4IV ACGT 3 cut(s) 435, 816, 865
HpyCH4V TGCA 3 cut(s) 106, 771, 918
HpyF3I CTNAG 3 cut(s) 62, 94, 236
HpySE526I ACGT 3 cut(s) 435, 816, 865
Hsp92I GRCGYC 1 cut(s) 620
Hsp92II CATG 2 cut(s) 85, 588
HspAI GCGC 5 cut(s) 85, 250, 582, 746, 765
KflI GGGWCCC 1 cut(s) 898
Kpn2I TCCGGA 1 cut(s) 242
Kzo9I GATC 4 cut(s) 376, 500, 654, 734
LmnI GCTCC 5 cut(s) 245, 497, 574, 695, 888
Lsp1109I GCAGC 6 cut(s) 118, 271, 410, 437, 473, 492
LweI GCATC 1 cut(s) 416
MaeI CTAG 1 cut(s) 215
MaeII ACGT 3 cut(s) 435, 816, 865
MaeIII GTNAC 4 cut(s) 31, 457, 551, 700
MalI GATC 4 cut(s) 378, 502, 656, 736
MboI GATC 4 cut(s) 376, 500, 654, 734
MboII GAAGA 1 cut(s) 45
MhlI GDGCHC 3 cut(s) 126, 242, 773
MlsI TGGCCA 2 cut(s) 23, 589
MluCI AATT 2 cut(s) 127, 603
MluNI TGGCCA 2 cut(s) 23, 589
Mox20I TGGCCA 2 cut(s) 23, 589
MroI TCCGGA 1 cut(s) 242
MscI TGGCCA 2 cut(s) 23, 589
MseI TTAA 2 cut(s) 150, 432
Msp20I TGGCCA 2 cut(s) 23, 589
MspA1I CMGCKG 1 cut(s) 486
MspCI CTTAAG 1 cut(s) 149
MspI CCGG 6 cut(s) 243, 447, 557, 572, 800, 887
MspR9I CCNGG 8 cut(s) 341, 557, 566, 573, 800, 801, 810, 887
MvaI CCWGG 3 cut(s) 341, 566, 810
MvnI CGCG 3 cut(s) 746, 767, 914
NciI CCSGG 5 cut(s) 557, 573, 800, 801, 887
NdeII GATC 4 cut(s) 376, 500, 654, 734
NlaIII CATG 2 cut(s) 85, 588
NlaIV GGNNCC 8 cut(s) 158, 310, 451, 493, 570, 697, 899, 900
NmuCI GTSAC 3 cut(s) 31, 457, 700
NsbI TGCGCA 2 cut(s) 86, 583
PfeI GAWTC 1 cut(s) 331
PfoI TCCNGGA 2 cut(s) 339, 808
PinAI ACCGGT 1 cut(s) 446
Ppu21I YACGTR 1 cut(s) 866
PpuMI RGGWCCY 1 cut(s) 898
Psp124BI GAGCTC 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 898
Psp6I CCWGG 3 cut(s) 339, 564, 808
PspEI GGTNACC 1 cut(s) 551
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 3 cut(s) 339, 564, 808
PspN4I GGNNCC 8 cut(s) 158, 310, 451, 493, 570, 697, 899, 900
PspPI GGNCC 5 cut(s) 140, 207, 308, 611, 898
PspPPI RGGWCCY 1 cut(s) 898
PsrI GAACNNNNNNTAC 2 cut(s) 173, 205
PstNI CAGNNNCTG 3 cut(s) 69, 235, 486
RsaI GTAC 3 cut(s) 73, 182, 864
RsaNI GTAC 3 cut(s) 72, 181, 863
SacI GAGCTC 1 cut(s) 242
SalI GTCGAC 1 cut(s) 218
SaqAI TTAA 2 cut(s) 150, 432
Sau3AI GATC 4 cut(s) 376, 500, 654, 734
Sau96I GGNCC 5 cut(s) 140, 207, 308, 611, 898
ScrFI CCNGG 8 cut(s) 341, 557, 566, 573, 800, 801, 810, 887
SduI GDGCHC 3 cut(s) 126, 242, 773
SfaNI GCATC 1 cut(s) 416
SfcI CTRYAG 1 cut(s) 475
SinI GGWCC 3 cut(s) 140, 207, 898
SmaI CCCGGG 1 cut(s) 801
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SpeI ACTAGT 1 cut(s) 214
Sse9I AATT 2 cut(s) 127, 603
SsiI CCGC 8 cut(s) 116, 257, 484, 489, 614, 692, 758, 912
SspI AATATT 1 cut(s) 878
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 242
StyD4I CCNGG 8 cut(s) 339, 555, 564, 571, 798, 799, 808, 885
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 4 cut(s) 166, 207, 463, 685
TaiI ACGT 3 cut(s) 438, 819, 868
TaqI TCGA 3 cut(s) 219, 657, 737
TasI AATT 2 cut(s) 127, 603
TatI WGTACW 1 cut(s) 71
TauI GCSGC 4 cut(s) 118, 486, 492, 616
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 2 cut(s) 150, 432
Tru9I TTAA 2 cut(s) 150, 432
TscAI CASTG 2 cut(s) 584, 778
TseFI GTSAC 3 cut(s) 31, 457, 700
TseI GCWGC 6 cut(s) 106, 259, 398, 425, 480, 486
Tsp45I GTSAC 3 cut(s) 31, 457, 700
TspGWI ACGGA 5 cut(s) 256, 513, 615, 675, 735
TspMI CCCGGG 1 cut(s) 799
TspRI CASTG 2 cut(s) 584, 778
Vha464I CTTAAG 1 cut(s) 149
VneI GTGCAC 1 cut(s) 769
VpaK11BI GGWCC 3 cut(s) 140, 207, 898
XmaI CCCGGG 1 cut(s) 799
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.