FvH4_5g36960

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002144
Physical Location & Seq
Forward (+)
27052349 .. 27053625
1277 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36960.t1

Sequence Viewer

Length: 954 bp
ATGTCGAAATCAATGGGTTCCTCCCAATTTTCTAAACCAGTAGCTCGTCTTCTTGTTCTTGTTCTTGTTCAGTTTCTCTTCTTAAGTAATGTGTGTCTTGGAGCAAGAAAGCTAACGTCACTGTACCAACCTCCAACCATGGCTCTAACCTACCACGGTGGCGCTTTGCTCCAAGGAGACCTCCCAGTCTCTATACTCTGGTACGGGAACTTCTCGCCCGCCCAGAAATCCATCATTGCCGACTTTCTTCTCTCCCTTAATCACAAAGATCAAAAGACAGCTAAGCCCTCAGTCACTCAATGGTGGAACTCAGTCCAAACTTACATGAAAAAAGCCGGCCAAAAACCAACCAATGTTTCTCTTTCTACCCAACTCTCAGACCACAACTGCTCTCTGGGCAAAACCCTAAAAAAATCTCAGATCACCGAGTTGGCTCGCCGTGCCAACTTCGGCCGGGGATGGTTGACACTTGTACTGACAGCACCAGATGTAGCGGTGGAAGGGTTTTGCATGCGCAACTGCGGGTTTCACGACTCGAACCGGAAGTCCAACTCGGTGTTCATCTGGGTCGGAAACTCGGCGACTCAGTGCCCGGGTAAGTGCGCGTGGCCATTCCATCAGCCCATATACGGGCCACAAACCCCGCCGTTGGTTGCCCCCAATGCTGACGTGGGGGTCGATGGCATGGTGGTAAATATCGCCACTCTTTTGGCGGGAACAGTGACAAACCCGTTTGCAAACGGGTTTTACCTCGGTTCAACAGAGGCGCCACTAGAGGCGGCTTCCGCTTGTACCGGGGTTTACGGCAAGGGGGCGTACCCAGGTTATGCGGGAGAGCTGCTGGTGGATGCAAACAGTGGCGCGAGCTACAATGCGGTGGGCGTCAATGGGTGGAAGTACCTCTTGCCTGCTTTGTTTGATCCTTATACTTCTCAGTGTGAGACCATTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

318

Amino Acids

34.08

Weight (kDa)

9.04

Isoelectric Point (pI)

30.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 49 - 315 2.5e-119 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 185, 674
Acc16I TGCGCA 1 cut(s) 515
AccB1I GGYRCC 1 cut(s) 766
AccII CGCG 2 cut(s) 605, 863
AciI CCGC 9 cut(s) 219, 494, 522, 644, 713, 779, 786, 830, 875
AclWI GGATC 1 cut(s) 914
AcoI YGGCCR 3 cut(s) 337, 451, 608
AcyI GRCGYC 2 cut(s) 767, 882
AfaI GTAC 6 cut(s) 125, 203, 474, 793, 818, 899
AfiI CCNNNNNNNGG 3 cut(s) 629, 630, 649
AflII CTTAAG 1 cut(s) 82
AgsI TTSAA 1 cut(s) 759
AjiI CACGTC 1 cut(s) 670
AjnI CCWGG 1 cut(s) 820
AjuI GAANNNNNNNTTGG 4 cut(s) 340, 372, 542, 574
AloI GAACNNNNNNTCC 2 cut(s) 530, 562
AluBI AGCT 5 cut(s) 44, 112, 281, 838, 867
AluI AGCT 5 cut(s) 44, 112, 281, 838, 867
Alw26I GTCTC 3 cut(s) 171, 193, 935
AlwI GGATC 1 cut(s) 914
Ama87I CYCGRG 1 cut(s) 592
AoxI GGCC 4 cut(s) 337, 451, 608, 632
ApeKI GCWGC 1 cut(s) 838
ArsI GACNNNNNNTTYG 2 cut(s) 530, 562
AspLEI GCGC 5 cut(s) 164, 516, 605, 769, 863
AspS9I GGNCC 1 cut(s) 632
AsuC2I CCSGG 4 cut(s) 455, 593, 594, 796
AsuHPI GGTGA 1 cut(s) 415
AvaI CYCGRG 1 cut(s) 592
BaeGI GKGCMC 1 cut(s) 593
BalI TGGCCA 1 cut(s) 610
BanI GGYRCC 1 cut(s) 766
BarI GAAGNNNNNNTAC 4 cut(s) 33, 65, 194, 226
BbsI GAAGAC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 825
BccI CCATC 4 cut(s) 239, 453, 624, 674
BceAI ACGGC 3 cut(s) 423, 631, 820
BciT130I CCWGG 1 cut(s) 822
BcnI CCSGG 4 cut(s) 455, 593, 594, 796
BcoDI GTCTC 3 cut(s) 171, 193, 935
BfaI CTAG 1 cut(s) 773
BfoI RGCGCY 2 cut(s) 165, 770
BfrI CTTAAG 1 cut(s) 82
BisI GCNGC 2 cut(s) 780, 839
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 2 cut(s) 781, 840
Bme1390I CCNGG 5 cut(s) 455, 593, 594, 796, 822
BmeT110I CYCGRG 1 cut(s) 592
BmgBI CACGTC 1 cut(s) 670
BmgT120I GGNCC 1 cut(s) 632
BmiI GGNNCC 2 cut(s) 19, 768
BmrFI CCNGG 5 cut(s) 455, 593, 594, 796, 822
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 1 cut(s) 838
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 41
Bpu1102I GCTNAGC 1 cut(s) 282
BpuMI CCSGG 4 cut(s) 455, 593, 594, 796
BsaHI GRCGYC 2 cut(s) 767, 882
BsaI GGTCTC 2 cut(s) 171, 935
BsaJI CCNNGG 8 cut(s) 138, 154, 172, 454, 592, 751, 795, 820
BsaWI WCCGGW 1 cut(s) 540
Bsc4I CCNNNNNNNGG 3 cut(s) 629, 630, 649
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 38, 185
Bse3DI GCAATG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 822
BseDI CCNNGG 8 cut(s) 138, 154, 172, 454, 592, 751, 795, 820
BseGI GGATG 2 cut(s) 464, 853
BseLI CCNNNNNNNGG 3 cut(s) 629, 630, 649
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 6 cut(s) 303, 324, 390, 431, 599, 947
BseNI ACTGG 2 cut(s) 38, 185
BseSI GKGCMC 1 cut(s) 593
BseX3I CGGCCG 1 cut(s) 451
BseXI GCAGC 1 cut(s) 825
Bsh1236I CGCG 2 cut(s) 605, 863
Bsh1285I CGRYCG 1 cut(s) 454
BshFI GGCC 4 cut(s) 339, 453, 610, 634
BshNI GGYRCC 1 cut(s) 766
BsiEI CGRYCG 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 592
BsiSI CCGG 5 cut(s) 336, 454, 541, 593, 795
BslI CCNNNNNNNGG 3 cut(s) 629, 630, 649
BsmAI GTCTC 3 cut(s) 171, 193, 935
BsnI GGCC 4 cut(s) 339, 453, 610, 634
Bso31I GGTCTC 2 cut(s) 171, 935
BsoBI CYCGRG 1 cut(s) 592
Bsp1286I GDGCHC 1 cut(s) 593
Bsp143I GATC 3 cut(s) 268, 420, 919
Bsp1720I GCTNAGC 1 cut(s) 282
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 9 cut(s) 219, 494, 522, 644, 713, 779, 786, 830, 875
BspANI GGCC 4 cut(s) 339, 453, 610, 634
BspCNI CTCAG 6 cut(s) 302, 323, 389, 430, 598, 946
BspFNI CGCG 2 cut(s) 605, 863
BspLI GGNNCC 2 cut(s) 19, 768
BspPI GGATC 1 cut(s) 914
BspT107I GGYRCC 1 cut(s) 766
BspTI CTTAAG 1 cut(s) 82
BspTNI GGTCTC 2 cut(s) 171, 935
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 335
BsrI ACTGG 2 cut(s) 38, 185
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 8 cut(s) 138, 154, 172, 454, 592, 751, 795, 820
BssMI GATC 3 cut(s) 268, 420, 919
BssNI GRCGYC 2 cut(s) 767, 882
BssT1I CCWWGG 2 cut(s) 138, 172
Bst2UI CCWGG 1 cut(s) 822
Bst4CI ACNGT 4 cut(s) 123, 158, 721, 857
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 2 cut(s) 767, 882
BstAFI CTTAAG 1 cut(s) 82
BstC8I GCNNGC 6 cut(s) 219, 337, 436, 512, 865, 909
BstDEI CTNAG 7 cut(s) 282, 289, 310, 376, 417, 585, 933
BstDSI CCRYGG 2 cut(s) 138, 154
BstF5I GGATG 2 cut(s) 464, 853
BstFNI CGCG 2 cut(s) 605, 863
BstH2I RGCGCY 2 cut(s) 165, 770
BstHHI GCGC 5 cut(s) 164, 516, 605, 769, 863
BstKTI GATC 3 cut(s) 271, 423, 922
BstMAI GTCTC 3 cut(s) 171, 193, 935
BstMBI GATC 3 cut(s) 268, 420, 919
BstMCI CGRYCG 1 cut(s) 454
BstMWI GCNNNNNNNGC 4 cut(s) 396, 440, 662, 785
BstNI CCWGG 1 cut(s) 822
BstNSI RCATGY 1 cut(s) 514
BstSCI CCNGG 5 cut(s) 453, 591, 592, 794, 820
BstSLI GKGCMC 1 cut(s) 593
BstUI CGCG 2 cut(s) 605, 863
BstV1I GCAGC 1 cut(s) 825
BstV2I GAAGAC 1 cut(s) 41
BstXI CCANNNNNNTGG 1 cut(s) 709
BstZI CGGCCG 1 cut(s) 451
BsuRI GGCC 4 cut(s) 339, 453, 610, 634
BtgI CCRYGG 2 cut(s) 138, 154
BtrI CACGTC 1 cut(s) 670
BtsCI GGATG 2 cut(s) 464, 853
BtsIMutI CAGTG 5 cut(s) 119, 593, 726, 862, 941
Cac8I GCNNGC 6 cut(s) 219, 337, 436, 512, 865, 909
CfoI GCGC 5 cut(s) 164, 516, 605, 769, 863
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 632
Cfr9I CCCGGG 1 cut(s) 592
CseI GACGC 1 cut(s) 871
Csp6I GTAC 6 cut(s) 124, 202, 473, 792, 817, 898
CviAII CATG 4 cut(s) 139, 325, 511, 685
CviQI GTAC 6 cut(s) 124, 202, 473, 792, 817, 898
DdeI CTNAG 7 cut(s) 282, 289, 310, 376, 417, 585, 933
DinI GGCGCC 1 cut(s) 768
DpnI GATC 3 cut(s) 270, 422, 921
DpnII GATC 3 cut(s) 268, 420, 919
DrdI GACNNNNNNGTC 2 cut(s) 185, 674
DseDI GACNNNNNNGTC 2 cut(s) 185, 674
EaeI YGGCCR 3 cut(s) 337, 451, 608
EagI CGGCCG 1 cut(s) 451
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EclXI CGGCCG 1 cut(s) 451
Eco130I CCWWGG 2 cut(s) 138, 172
Eco31I GGTCTC 2 cut(s) 171, 935
Eco52I CGGCCG 1 cut(s) 451
Eco88I CYCGRG 1 cut(s) 592
EcoRII CCWGG 1 cut(s) 820
EcoT14I CCWWGG 2 cut(s) 138, 172
EgeI GGCGCC 1 cut(s) 768
EheI GGCGCC 1 cut(s) 768
ErhI CCWWGG 2 cut(s) 138, 172
FaeI CATG 4 cut(s) 142, 328, 514, 688
FaiI YATR 9 cut(s) 140, 194, 326, 512, 626, 628, 686, 828, 927
FalI AAGNNNNNCTT 2 cut(s) 887, 919
FatI CATG 4 cut(s) 138, 324, 510, 684
FauI CCCGC 5 cut(s) 226, 515, 651, 706, 823
Fnu4HI GCNGC 2 cut(s) 780, 839
FokI GGATG 2 cut(s) 471, 860
Fsp4HI GCNGC 2 cut(s) 780, 839
FspBI CTAG 1 cut(s) 773
FspI TGCGCA 1 cut(s) 515
GlaI GCGC 5 cut(s) 163, 515, 604, 768, 862
GluI GCNGC 2 cut(s) 780, 839
HaeII RGCGCY 2 cut(s) 165, 770
HaeIII GGCC 4 cut(s) 339, 453, 610, 634
HapII CCGG 5 cut(s) 336, 454, 541, 593, 795
HgaI GACGC 1 cut(s) 871
HhaI GCGC 5 cut(s) 164, 516, 605, 769, 863
Hin1I GRCGYC 2 cut(s) 767, 882
Hin1II CATG 4 cut(s) 142, 328, 514, 688
Hin6I GCGC 5 cut(s) 162, 514, 603, 767, 861
HinP1I GCGC 5 cut(s) 162, 514, 603, 767, 861
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HinfI GANTC 2 cut(s) 533, 583
HpaII CCGG 5 cut(s) 336, 454, 541, 593, 795
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 2 cut(s) 465, 802
Hpy188I TCNGA 3 cut(s) 379, 420, 572
Hpy188III TCNNGA 1 cut(s) 530
Hpy8I GTNNAC 2 cut(s) 465, 802
HpyAV CCTTC 1 cut(s) 494
HpyCH4III ACNGT 4 cut(s) 123, 158, 721, 857
HpyCH4IV ACGT 2 cut(s) 116, 669
HpyCH4V TGCA 3 cut(s) 510, 737, 851
HpyF10VI GCNNNNNNNGC 4 cut(s) 396, 440, 662, 785
HpyF3I CTNAG 7 cut(s) 282, 289, 310, 376, 417, 585, 933
HpySE526I ACGT 2 cut(s) 116, 669
Hsp92I GRCGYC 2 cut(s) 767, 882
Hsp92II CATG 4 cut(s) 142, 328, 514, 688
HspAI GCGC 5 cut(s) 162, 514, 603, 767, 861
KasI GGCGCC 1 cut(s) 766
KroI GCCGGC 1 cut(s) 335
KroNI GCCGGC 1 cut(s) 337
Kzo9I GATC 3 cut(s) 268, 420, 919
LmnI GCTCC 2 cut(s) 101, 174
Lsp1109I GCAGC 1 cut(s) 825
LweI GCATC 1 cut(s) 838
MaeI CTAG 1 cut(s) 773
MaeII ACGT 2 cut(s) 116, 669
MaeIII GTNAC 3 cut(s) 117, 292, 721
MalI GATC 3 cut(s) 270, 422, 921
MboI GATC 3 cut(s) 268, 420, 919
MboII GAAGA 3 cut(s) 41, 70, 239
MhlI GDGCHC 1 cut(s) 593
MlsI TGGCCA 1 cut(s) 610
MluCI AATT 1 cut(s) 26
MluNI TGGCCA 1 cut(s) 610
Mly113I GGCGCC 1 cut(s) 767
MlyI GAGTC 2 cut(s) 527, 577
MmeI TCCRAC 3 cut(s) 158, 550, 573
MnlI CCTC 8 cut(s) 31, 141, 191, 298, 757, 761, 769, 911
Mox20I TGGCCA 1 cut(s) 610
MroNI GCCGGC 1 cut(s) 335
MscI TGGCCA 1 cut(s) 610
MseI TTAA 2 cut(s) 83, 258
Msp20I TGGCCA 1 cut(s) 610
MspCI CTTAAG 1 cut(s) 82
MspI CCGG 5 cut(s) 336, 454, 541, 593, 795
MspR9I CCNGG 5 cut(s) 455, 593, 594, 796, 822
MvaI CCWGG 1 cut(s) 822
MvnI CGCG 2 cut(s) 605, 863
MwoI GCNNNNNNNGC 4 cut(s) 396, 440, 662, 785
NaeI GCCGGC 1 cut(s) 337
NarI GGCGCC 1 cut(s) 767
NciI CCSGG 4 cut(s) 455, 593, 594, 796
NcoI CCATGG 1 cut(s) 138
NdeII GATC 3 cut(s) 268, 420, 919
NgoMIV GCCGGC 1 cut(s) 335
NlaIII CATG 4 cut(s) 142, 328, 514, 688
NlaIV GGNNCC 2 cut(s) 19, 768
NmeAIII GCCGAG 1 cut(s) 557
NmuCI GTSAC 3 cut(s) 117, 292, 721
NsbI TGCGCA 1 cut(s) 515
NspI RCATGY 1 cut(s) 514
PaeI GCATGC 1 cut(s) 514
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PdiI GCCGGC 1 cut(s) 337
PkrI GCNGC 2 cut(s) 781, 840
PleI GAGTC 2 cut(s) 527, 577
PluTI GGCGCC 1 cut(s) 770
PpsI GAGTC 2 cut(s) 527, 577
Psp6I CCWGG 1 cut(s) 820
PspGI CCWGG 1 cut(s) 820
PspN4I GGNNCC 2 cut(s) 19, 768
PspPI GGNCC 1 cut(s) 632
RsaI GTAC 6 cut(s) 125, 203, 474, 793, 818, 899
RsaNI GTAC 6 cut(s) 124, 202, 473, 792, 817, 898
SaqAI TTAA 2 cut(s) 83, 258
SatI GCNGC 2 cut(s) 780, 839
Sau3AI GATC 3 cut(s) 268, 420, 919
Sau96I GGNCC 1 cut(s) 632
SchI GAGTC 2 cut(s) 527, 577
ScrFI CCNGG 5 cut(s) 455, 593, 594, 796, 822
SduI GDGCHC 1 cut(s) 593
SfaNI GCATC 1 cut(s) 838
SfoI GGCGCC 1 cut(s) 768
SmaI CCCGGG 1 cut(s) 594
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
SphI GCATGC 1 cut(s) 514
Sse9I AATT 1 cut(s) 26
SsiI CCGC 9 cut(s) 219, 494, 522, 644, 713, 779, 786, 830, 875
SspDI GGCGCC 1 cut(s) 766
SspMI CTAG 1 cut(s) 773
StyD4I CCNGG 5 cut(s) 453, 591, 592, 794, 820
StyI CCWWGG 2 cut(s) 138, 172
TaaI ACNGT 4 cut(s) 123, 158, 721, 857
TaiI ACGT 2 cut(s) 119, 672
TaqI TCGA 3 cut(s) 5, 536, 678
TasI AATT 1 cut(s) 26
TatI WGTACW 1 cut(s) 472
TauI GCSGC 1 cut(s) 782
Tru1I TTAA 2 cut(s) 83, 258
Tru9I TTAA 2 cut(s) 83, 258
TscAI CASTG 5 cut(s) 126, 593, 726, 862, 941
TseFI GTSAC 3 cut(s) 117, 292, 721
TseI GCWGC 1 cut(s) 838
Tsp45I GTSAC 3 cut(s) 117, 292, 721
TspDTI ATGAA 2 cut(s) 341, 550
TspMI CCCGGG 1 cut(s) 592
TspRI CASTG 5 cut(s) 126, 593, 726, 862, 941
Vha464I CTTAAG 1 cut(s) 82
XceI RCATGY 1 cut(s) 514
XcmI CCANNNNNNNNNTGG 1 cut(s) 667
XmaI CCCGGG 1 cut(s) 592
XspI CTAG 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.