Rh7DG461000

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0002144
Physical Location & Seq
Forward (+)
65492803 .. 65493973
1171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG461000.1

Sequence Viewer

Length: 960 bp
ATGTGGAAATTAATGGGTTCCTCACAATTTTCTAAACCAGTAGCTCGTCTTCTTGTTCTTCTTCAATTTCTCTTAACAAGTAATGTGTGTATTGGAGCAAGAAAGCTGACGTCACTGTACCAACCTCCACCCATGGCTCTGACCTATCATGGTGGCACTTTGCTCCAAGGAGACCTCCCAGTCTCTATACTCTGGTATGGAAACTTCTCACCAGCCCAGAAATCCACTGTGGCAGACTTTCTTCTCTCCCTTAAAAACAACCCTCACAAAAATCAAAAGACAGCTAAACCTTCAGTCACTCAATGGTGGAACTCAGTCGAAACCTACATGAAAAAAGCCGGCCAAAAACCAACCCATGTTTCTCTTTCTACCCAACTCTCAGACCACAACTGCTCTCTGGGAAAAACCCTAAAAAAATCTCAAATCTCCGAGTTAGCCCACCGGGTCAACTCGCCCAACTCGGGTGGATTGACACTTGTGCTCACTGCCCCAGATGTAGCGGTCGAAGGGTTTTGCATGAGCAACTGCGGGTTTCACGACTCGAACCGGAAGTCGAACTCGGTTTTCATCTGGGTCGGGAACTCGGCAACTCAGTGCCCGGGTCAGTGCGCGTGGCCATTCCATCAACCCATCTACGGACCACAAACCCCACCGTTGGTTGCACCCAATGCTGACGTGGGGGTGGATGGCATGGTGGTAAATATCGCTAGTCTTTTGGCGGGAACAGTGACAAATCCGTTTGCAAACGGGTATTACCTCGGGTCAGCGGAGGCGCCGCTAGAGGTGGCCTCCGCTTGTACCGGGATGTATGGAAAGGGGGCGTACCCGGGTTACGCGGGGGAGCTGCTGGTGGACTCGAGCAGTGGCGCGAGCTACAATGCGGTGGGCGTGAATGGGAGGAAGTACCTCTTGCCCGCTTTGTTTGATCCTTATACTTCTCAGTGTGAGGCCATTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

319

Amino Acids

34.11

Weight (kDa)

8.82

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 47 - 317 5.9e-121 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
AatII GACGTC 1 cut(s) 113
AccB1I GGYRCC 1 cut(s) 772
AccII CGCG 3 cut(s) 611, 836, 869
AciI CCGC 9 cut(s) 500, 528, 719, 767, 776, 792, 836, 881, 915
AclWI GGATC 1 cut(s) 920
AcoI YGGCCR 2 cut(s) 340, 614
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 2 cut(s) 110, 773
AfaI GTAC 4 cut(s) 119, 799, 824, 905
AfiI CCNNNNNNNGG 3 cut(s) 461, 635, 655
AgsI TTSAA 1 cut(s) 65
AjiI CACGTC 1 cut(s) 676
AjuI GAANNNNNNNTTGG 2 cut(s) 343, 375
AluBI AGCT 5 cut(s) 44, 106, 284, 844, 873
AluI AGCT 5 cut(s) 44, 106, 284, 844, 873
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 2 cut(s) 165, 187
AlwI GGATC 1 cut(s) 920
Ama87I CYCGRG 5 cut(s) 460, 598, 758, 826, 856
AoxI GGCC 4 cut(s) 340, 614, 786, 948
ApeKI GCWGC 1 cut(s) 844
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AseI ATTAAT 1 cut(s) 11
AspLEI GCGC 3 cut(s) 611, 775, 869
AspS9I GGNCC 1 cut(s) 638
AsuC2I CCSGG 6 cut(s) 443, 599, 600, 802, 827, 828
AsuHPI GGTGA 1 cut(s) 201
AvaI CYCGRG 5 cut(s) 460, 598, 758, 826, 856
AvaII GGWCC 1 cut(s) 638
BaeGI GKGCMC 1 cut(s) 599
BalI TGGCCA 1 cut(s) 616
BanI GGYRCC 1 cut(s) 772
BarI GAAGNNNNNNTAC 4 cut(s) 33, 65, 188, 220
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 1 cut(s) 831
BccI CCATC 3 cut(s) 630, 638, 680
BcnI CCSGG 6 cut(s) 443, 599, 600, 802, 827, 828
BcoDI GTCTC 2 cut(s) 165, 187
BfaI CTAG 2 cut(s) 708, 779
BfoI RGCGCY 1 cut(s) 776
BisI GCNGC 2 cut(s) 776, 845
BlsI GCNGC 2 cut(s) 777, 846
Bme1390I CCNGG 6 cut(s) 443, 599, 600, 802, 827, 828
Bme18I GGWCC 1 cut(s) 638
BmeT110I CYCGRG 5 cut(s) 460, 598, 758, 826, 856
BmgBI CACGTC 1 cut(s) 676
BmgT120I GGNCC 1 cut(s) 638
BmiI GGNNCC 2 cut(s) 19, 774
BmrFI CCNGG 6 cut(s) 443, 599, 600, 802, 827, 828
BmrI ACTGGG 1 cut(s) 173
BmuI ACTGGG 1 cut(s) 173
BpiI GAAGAC 1 cut(s) 41
BplI GAGNNNNNCTC 2 cut(s) 773, 805
BpuMI CCSGG 6 cut(s) 443, 599, 600, 802, 827, 828
BsaHI GRCGYC 2 cut(s) 110, 773
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 5 cut(s) 132, 166, 598, 757, 826
BsaWI WCCGGW 1 cut(s) 546
Bsc4I CCNNNNNNNGG 3 cut(s) 461, 635, 655
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 2 cut(s) 38, 179
BseDI CCNNGG 5 cut(s) 132, 166, 598, 757, 826
BseGI GGATG 2 cut(s) 691, 810
BseLI CCNNNNNNNGG 3 cut(s) 461, 635, 655
BseMII CTCAG 4 cut(s) 327, 393, 605, 953
BseNI ACTGG 2 cut(s) 38, 179
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 1 cut(s) 831
Bsh1236I CGCG 3 cut(s) 611, 836, 869
Bsh1285I CGRYCG 1 cut(s) 504
BshFI GGCC 4 cut(s) 342, 616, 788, 950
BshNI GGYRCC 1 cut(s) 772
BsiEI CGRYCG 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 5 cut(s) 460, 598, 758, 826, 856
BsiSI CCGG 6 cut(s) 339, 442, 547, 599, 801, 827
BslI CCNNNNNNNGG 3 cut(s) 461, 635, 655
BsmAI GTCTC 2 cut(s) 165, 187
BsnI GGCC 4 cut(s) 342, 616, 788, 950
Bso31I GGTCTC 1 cut(s) 165
BsoBI CYCGRG 5 cut(s) 460, 598, 758, 826, 856
Bsp1286I GDGCHC 2 cut(s) 483, 599
Bsp143I GATC 1 cut(s) 925
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 9 cut(s) 500, 528, 719, 767, 776, 792, 836, 881, 915
BspANI GGCC 4 cut(s) 342, 616, 788, 950
BspCNI CTCAG 4 cut(s) 326, 392, 604, 952
BspFNI CGCG 3 cut(s) 611, 836, 869
BspLI GGNNCC 2 cut(s) 19, 774
BspPI GGATC 1 cut(s) 920
BspT107I GGYRCC 1 cut(s) 772
BspTNI GGTCTC 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 2 cut(s) 38, 179
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 5 cut(s) 132, 166, 598, 757, 826
BssMI GATC 1 cut(s) 925
BssNI GRCGYC 2 cut(s) 110, 773
BssT1I CCWWGG 2 cut(s) 132, 166
Bst4CI ACNGT 4 cut(s) 117, 229, 654, 727
BstACI GRCGYC 2 cut(s) 110, 773
BstAPI GCANNNNNTGC 1 cut(s) 668
BstC8I GCNNGC 3 cut(s) 340, 871, 915
BstDEI CTNAG 4 cut(s) 313, 379, 591, 939
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 2 cut(s) 691, 810
BstFNI CGCG 3 cut(s) 611, 836, 869
BstH2I RGCGCY 1 cut(s) 776
BstHHI GCGC 3 cut(s) 611, 775, 869
BstKTI GATC 1 cut(s) 928
BstMAI GTCTC 2 cut(s) 165, 187
BstMBI GATC 1 cut(s) 925
BstMCI CGRYCG 1 cut(s) 504
BstMWI GCNNNNNNNGC 1 cut(s) 668
BstSCI CCNGG 6 cut(s) 441, 597, 598, 800, 825, 826
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 3 cut(s) 611, 836, 869
BstV1I GCAGC 1 cut(s) 831
BstV2I GAAGAC 1 cut(s) 41
BsuRI GGCC 4 cut(s) 342, 616, 788, 950
BtgI CCRYGG 1 cut(s) 132
BtrI CACGTC 1 cut(s) 676
BtsCI GGATG 2 cut(s) 691, 810
BtsI GCAGTG 2 cut(s) 483, 868
BtsIMutI CAGTG 8 cut(s) 113, 225, 483, 599, 611, 732, 868, 947
Cac8I GCNNGC 3 cut(s) 340, 871, 915
CfoI GCGC 3 cut(s) 611, 775, 869
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 1 cut(s) 638
Cfr9I CCCGGG 2 cut(s) 598, 826
Csp6I GTAC 4 cut(s) 118, 798, 823, 904
CspCI CAANNNNNGTGG 2 cut(s) 445, 480
CviAII CATG 6 cut(s) 133, 149, 328, 356, 517, 691
CviQI GTAC 4 cut(s) 118, 798, 823, 904
DdeI CTNAG 4 cut(s) 313, 379, 591, 939
DinI GGCGCC 1 cut(s) 774
DpnI GATC 1 cut(s) 927
DpnII GATC 1 cut(s) 925
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
EaeI YGGCCR 2 cut(s) 340, 614
Eco130I CCWWGG 2 cut(s) 132, 166
Eco31I GGTCTC 1 cut(s) 165
Eco47I GGWCC 1 cut(s) 638
Eco57I CTGAAG 1 cut(s) 276
Eco88I CYCGRG 5 cut(s) 460, 598, 758, 826, 856
EcoT14I CCWWGG 2 cut(s) 132, 166
EgeI GGCGCC 1 cut(s) 774
EheI GGCGCC 1 cut(s) 774
ErhI CCWWGG 2 cut(s) 132, 166
FaeI CATG 6 cut(s) 136, 152, 331, 359, 520, 694
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FatI CATG 6 cut(s) 132, 148, 327, 355, 516, 690
FauI CCCGC 4 cut(s) 521, 712, 829, 922
Fnu4HI GCNGC 2 cut(s) 776, 845
FokI GGATG 2 cut(s) 698, 817
Fsp4HI GCNGC 2 cut(s) 776, 845
FspBI CTAG 2 cut(s) 708, 779
GlaI GCGC 3 cut(s) 610, 774, 868
GluI GCNGC 2 cut(s) 776, 845
HaeII RGCGCY 1 cut(s) 776
HaeIII GGCC 4 cut(s) 342, 616, 788, 950
HapII CCGG 6 cut(s) 339, 442, 547, 599, 801, 827
HhaI GCGC 3 cut(s) 611, 775, 869
Hin1I GRCGYC 2 cut(s) 110, 773
Hin1II CATG 6 cut(s) 136, 152, 331, 359, 520, 694
Hin6I GCGC 3 cut(s) 609, 773, 867
HinP1I GCGC 3 cut(s) 609, 773, 867
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 2 cut(s) 539, 854
HpaII CCGG 6 cut(s) 339, 442, 547, 599, 801, 827
HphI GGTGA 1 cut(s) 201
Hpy166II GTNNAC 2 cut(s) 448, 853
Hpy188I TCNGA 3 cut(s) 141, 382, 430
Hpy188III TCNNGA 2 cut(s) 536, 577
Hpy8I GTNNAC 2 cut(s) 448, 853
HpyAV CCTTC 2 cut(s) 300, 500
HpyCH4III ACNGT 4 cut(s) 117, 229, 654, 727
HpyCH4IV ACGT 2 cut(s) 110, 675
HpyCH4V TGCA 3 cut(s) 516, 662, 743
HpyF10VI GCNNNNNNNGC 1 cut(s) 668
HpyF3I CTNAG 4 cut(s) 313, 379, 591, 939
HpySE526I ACGT 2 cut(s) 110, 675
Hsp92I GRCGYC 2 cut(s) 110, 773
Hsp92II CATG 6 cut(s) 136, 152, 331, 359, 520, 694
HspAI GCGC 3 cut(s) 609, 773, 867
KasI GGCGCC 1 cut(s) 772
KroI GCCGGC 1 cut(s) 338
KroNI GCCGGC 1 cut(s) 340
Kzo9I GATC 1 cut(s) 925
LmnI GCTCC 3 cut(s) 95, 168, 841
Lsp1109I GCAGC 1 cut(s) 831
MaeI CTAG 2 cut(s) 708, 779
MaeII ACGT 2 cut(s) 110, 675
MaeIII GTNAC 4 cut(s) 111, 295, 727, 830
MalI GATC 1 cut(s) 927
MboI GATC 1 cut(s) 925
MboII GAAGA 4 cut(s) 41, 50, 53, 233
MhlI GDGCHC 2 cut(s) 483, 599
MlsI TGGCCA 1 cut(s) 616
MluCI AATT 3 cut(s) 8, 26, 65
MluNI TGGCCA 1 cut(s) 616
Mly113I GGCGCC 1 cut(s) 773
MlyI GAGTC 2 cut(s) 533, 848
Mox20I TGGCCA 1 cut(s) 616
MroNI GCCGGC 1 cut(s) 338
MscI TGGCCA 1 cut(s) 616
MseI TTAA 3 cut(s) 11, 74, 252
Msp20I TGGCCA 1 cut(s) 616
MspA1I CMGCKG 1 cut(s) 767
MspI CCGG 6 cut(s) 339, 442, 547, 599, 801, 827
MspR9I CCNGG 6 cut(s) 443, 599, 600, 802, 827, 828
MvnI CGCG 3 cut(s) 611, 836, 869
MwoI GCNNNNNNNGC 1 cut(s) 668
NaeI GCCGGC 1 cut(s) 340
NarI GGCGCC 1 cut(s) 773
NciI CCSGG 6 cut(s) 443, 599, 600, 802, 827, 828
NcoI CCATGG 1 cut(s) 132
NdeII GATC 1 cut(s) 925
NgoMIV GCCGGC 1 cut(s) 338
NlaIII CATG 6 cut(s) 136, 152, 331, 359, 520, 694
NlaIV GGNNCC 2 cut(s) 19, 774
NmeAIII GCCGAG 1 cut(s) 563
NmuCI GTSAC 3 cut(s) 111, 295, 727
PaeR7I CTCGAG 1 cut(s) 856
PdiI GCCGGC 1 cut(s) 340
PkrI GCNGC 2 cut(s) 777, 846
PleI GAGTC 2 cut(s) 533, 848
PluTI GGCGCC 1 cut(s) 776
PpsI GAGTC 2 cut(s) 533, 848
PshBI ATTAAT 1 cut(s) 11
PspN4I GGNNCC 2 cut(s) 19, 774
PspPI GGNCC 1 cut(s) 638
PspXI VCTCGAGB 1 cut(s) 856
RsaI GTAC 4 cut(s) 119, 799, 824, 905
RsaNI GTAC 4 cut(s) 118, 798, 823, 904
SaqAI TTAA 3 cut(s) 11, 74, 252
SatI GCNGC 2 cut(s) 776, 845
Sau3AI GATC 1 cut(s) 925
Sau96I GGNCC 1 cut(s) 638
SchI GAGTC 2 cut(s) 533, 848
ScrFI CCNGG 6 cut(s) 443, 599, 600, 802, 827, 828
SduI GDGCHC 2 cut(s) 483, 599
SfoI GGCGCC 1 cut(s) 774
Sfr274I CTCGAG 1 cut(s) 856
SinI GGWCC 1 cut(s) 638
SlaI CTCGAG 1 cut(s) 856
SmaI CCCGGG 2 cut(s) 600, 828
SmlI CTYRAG 1 cut(s) 856
SmoI CTYRAG 1 cut(s) 856
Sse9I AATT 3 cut(s) 8, 26, 65
SsiI CCGC 9 cut(s) 500, 528, 719, 767, 776, 792, 836, 881, 915
SspDI GGCGCC 1 cut(s) 772
SspMI CTAG 2 cut(s) 708, 779
StyD4I CCNGG 6 cut(s) 441, 597, 598, 800, 825, 826
StyI CCWWGG 2 cut(s) 132, 166
TaaI ACNGT 4 cut(s) 117, 229, 654, 727
TaiI ACGT 2 cut(s) 113, 678
TaqI TCGA 5 cut(s) 318, 504, 542, 554, 857
TasI AATT 3 cut(s) 8, 26, 65
TauI GCSGC 1 cut(s) 778
Tru1I TTAA 3 cut(s) 11, 74, 252
Tru9I TTAA 3 cut(s) 11, 74, 252
TscAI CASTG 8 cut(s) 120, 232, 490, 599, 611, 732, 868, 947
TseFI GTSAC 3 cut(s) 111, 295, 727
TseI GCWGC 1 cut(s) 844
Tsp45I GTSAC 3 cut(s) 111, 295, 727
TspDTI ATGAA 2 cut(s) 344, 556
TspGWI ACGGA 2 cut(s) 651, 726
TspMI CCCGGG 2 cut(s) 598, 826
TspRI CASTG 8 cut(s) 120, 232, 490, 599, 611, 732, 868, 947
VpaK11BI GGWCC 1 cut(s) 638
VspI ATTAAT 1 cut(s) 11
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XhoI CTCGAG 1 cut(s) 856
XmaI CCCGGG 2 cut(s) 598, 826
XspI CTAG 2 cut(s) 708, 779
ZraI GACGTC 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.