Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002144

Physical Location & Seq

Genomic Coordinates Reverse (-)

7300835 .. 7301716

Gene Structure 882 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1042900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 882 bp

ATGTATCCGATTTACCATTTTGCCACTGCTCTCTTGCTCCTTCTTTTGGTGGCGAAGCCCAGTTTTGGCGCTTTAGTGGAGGAGCAGCCACTGGTGCTCAAGTACCACAACGGCGCTCTTCTTAAGGGCAACATCCCTGTCAATCTTATTTGGTACGGCAGCTTCACTCCTATCCAAAGGTCCATCATCGTCGACTTCATCCACTCCCTCGCTCCCCGGCGTTCCCCACTCCCCTCCGCCTCCTCCTGGTGGAAAACCACCGAGAAGTACAGGGGCGGCGCCACCAATCTCCTCGTCGGCAGGCAAATCCTCCACGAGGCCTACTCTCTCGGAAAGTCCCTCCGTAACCGACACCTGGTGGCCCTCGCCGGCAAAGTCAACGCGTTGAACGCCATCAACGTTGTTTTGACTGCCAGTGACGTGGCGGTCGACGGCTTCTGTAGCCGCTGCGGGACCCACGGCTCCAACCGGGATAAAAAGACGGCTTACGTGTGGGTTGGGAACTCGGTGGTCCAGTGCCCCGGCCAATGCGCCTGGCCCTTTCACCAGCCCATTTACGGCCCGCAAACTCCGCCGTTAGTGGCTCCGAACGGCGACGTTGGAGTTGATGGCATGATCATAAACCTCGCCACTCTCTTGGCGGGAGCCGTAACCAATCCATACAATAATGGGTACTTCCAAGGGCCGTCCAGCGCGCCACTCGAGGCCGTTTCTGCCTGCACCGGCGTTTTCGGAACCGGGGCCTACCCCGGGTATCCGGGTCGGGTTCTGGTGGATAAGGCGACCGGGGCGAGCTACAACGCGGTTGGGGTAAACGGGCGTAGGTTCCTATTGCCAGCCATGTGGGACCCGCAAACCTCTGCATGTAAGCCGCTTCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002239 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005829 GO:0005911 GO:0008150 GO:0009505 GO:0009506 GO:0009605 GO:0009607 GO:0012505 GO:0016020 GO:0030054 GO:0030312 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Phi_1

Protein Analysis

294

Amino Acids

31.36

Weight (kDa)

9.39

Isoelectric Point (pI)

29.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phi_1	PF04674	33 - 290	6.3e-118	Phosphate-induced protein 1 conserved region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64260
fragaria_vesca	FvH4_5g36850 FvH4_5g36960
malus_domestica	MD05G1042900.v1.1 MD08G1186400.v1.1 MD10G1050200.v1.1
prunus_persica	Prupe.1G520500_v2.0.a1 Prupe.1G520900_v2.0.a1 Prupe.8G064100_v2.0.a1
pyrus_communis	pycom05g03550 pycom08g16020 pycom10g03530
rosa_chinensis	RchiOBHm_Chr6g0271521 RchiOBHm_Chr7g0243431 RchiOBHm_Chr7g0243701
rosa_laevigata	RLG00000000839 RLG00000000847 RLG00000013724
rosa_multiflora	Rmu_sc0001036.1_g000013 Rmu_sc0008223.1_g000011 Rmu_sc0032000.1_g000003
rosa_roxburghii	Rroxscaffold_3G00222000 Rroxscaffold_3G00222110 Rroxscaffold_7G00196930
rosa_rugosa	Rorug06G0061200 Rorug07G0319200 Rorug07G0320400
rosa_samantha	Rh6AG180400 Rh6CG180200 Rh6DG172100 Rh7AG475300 Rh7AG475900 Rh7BG445200 Rh7BG446600 Rh7BG446800 Rh7BG446900 Rh7CG491500 Rh7CG492900 Rh7DG459800 Rh7DG461000
rosa_wichuraiana	Rw6G015500 Rw6G015570 Rw7G039550 Rw7G039640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	425
AccB1I	GGYRCC	1 cut(s)	278
AccI	GTMKAC	2 cut(s)	192, 429
AccII	CGCG	3 cut(s)	383, 695, 803
AclI	AACGTT	1 cut(s)	399
AcoI	YGGCCR	1 cut(s)	523
AcyI	GRCGYC	1 cut(s)	279
AdeI	CACNNNGTG	1 cut(s)	358
AfaI	GTAC	4 cut(s)	104, 155, 269, 674
AfiI	CCNNNNNNNGG	8 cut(s)	46, 65, 246, 249, 316, 355, 557, 750
AflII	CTTAAG	1 cut(s)	122
AflIII	ACRYGT	2 cut(s)	381, 489
AgsI	TTSAA	1 cut(s)	388
AjiI	CACGTC	1 cut(s)	421
AjnI	CCWGG	3 cut(s)	245, 354, 533
AluBI	AGCT	2 cut(s)	162, 795
AluI	AGCT	2 cut(s)	162, 795
Alw21I	GWGCWC	1 cut(s)	99
AlwNI	CAGNNNCTG	1 cut(s)	91
Ama87I	CYCGRG	2 cut(s)	701, 749
AoxI	GGCC	8 cut(s)	318, 360, 523, 536, 559, 683, 705, 741
ApeKI	GCWGC	3 cut(s)	85, 159, 447
AspLEI	GCGC	6 cut(s)	71, 116, 281, 533, 695, 697
AspS9I	GGNCC	9 cut(s)	180, 361, 453, 511, 537, 560, 683, 741, 847
AsuC2I	CCSGG	8 cut(s)	217, 470, 522, 739, 750, 751, 759, 787
AsuHPI	GGTGA	1 cut(s)	536
AvaI	CYCGRG	2 cut(s)	701, 749
AvaII	GGWCC	4 cut(s)	180, 453, 511, 847
BaeGI	GKGCMC	1 cut(s)	521
BanI	GGYRCC	1 cut(s)	278
BarI	GAAGNNNNNNTAC	2 cut(s)	146, 178
BauI	CACGAG	1 cut(s)	314
Bbv12I	GWGCWC	1 cut(s)	99
BbvI	GCAGC	3 cut(s)	97, 171, 434
BccI	CCATC	3 cut(s)	191, 401, 602
BciT130I	CCWGG	3 cut(s)	247, 356, 535
BciVI	GTATCC	2 cut(s)	15, 765
BclI	TGATCA	1 cut(s)	615
BcnI	CCSGG	8 cut(s)	217, 470, 522, 739, 750, 751, 759, 787
BfmI	CTRYAG	1 cut(s)	439
BfoI	RGCGCY	3 cut(s)	72, 117, 282
BfrI	CTTAAG	1 cut(s)	122
BfuI	GTATCC	2 cut(s)	15, 765
BisI	GCNGC	6 cut(s)	86, 160, 277, 445, 448, 872
BlsI	GCNGC	6 cut(s)	87, 161, 278, 446, 449, 873
Bme18I	GGWCC	4 cut(s)	180, 453, 511, 847
BmeT110I	CYCGRG	2 cut(s)	701, 749
BmgBI	CACGTC	1 cut(s)	421
BmgT120I	GGNCC	9 cut(s)	180, 361, 453, 511, 537, 560, 683, 741, 847
BmrI	ACTGGG	1 cut(s)	54
BmuI	ACTGGG	1 cut(s)	54
BplI	GAGNNNNNCTC	2 cut(s)	308, 340
BpuEI	CTTGAG	1 cut(s)	83
BpuMI	CCSGG	8 cut(s)	217, 470, 522, 739, 750, 751, 759, 787
BsaAI	YACGTR	1 cut(s)	490
BsaHI	GRCGYC	1 cut(s)	279
BsaJI	CCNNGG	8 cut(s)	215, 457, 520, 679, 738, 748, 749, 786
Bsc4I	CCNNNNNNNGG	8 cut(s)	46, 65, 246, 249, 316, 355, 557, 750
Bse118I	RCCGGY	2 cut(s)	368, 722
Bse1I	ACTGG	4 cut(s)	60, 96, 414, 514
BseBI	CCWGG	3 cut(s)	247, 356, 535
BseDI	CCNNGG	8 cut(s)	215, 457, 520, 679, 738, 748, 749, 786
BseGI	GGATG	2 cut(s)	132, 198
BseLI	CCNNNNNNNGG	8 cut(s)	46, 65, 246, 249, 316, 355, 557, 750
BseNI	ACTGG	4 cut(s)	60, 96, 414, 514
BsePI	GCGCGC	1 cut(s)	693
BseRI	GAGGAG	3 cut(s)	95, 232, 281
BseSI	GKGCMC	1 cut(s)	521
BseXI	GCAGC	3 cut(s)	97, 171, 434
BsgI	GTGCAG	1 cut(s)	703
Bsh1236I	CGCG	3 cut(s)	383, 695, 803
Bsh1285I	CGRYCG	2 cut(s)	429, 786
BshFI	GGCC	8 cut(s)	320, 362, 525, 538, 561, 685, 707, 743
BshNI	GGYRCC	1 cut(s)	278
BsiEI	CGRYCG	2 cut(s)	429, 786
BsiHKAI	GWGCWC	1 cut(s)	99
BsiHKCI	CYCGRG	2 cut(s)	701, 749
BsiSI	CCGG	9 cut(s)	217, 369, 469, 522, 723, 738, 750, 758, 786
BslFI	GGGAC	3 cut(s)	322, 466, 860
BslI	CCNNNNNNNGG	8 cut(s)	46, 65, 246, 249, 316, 355, 557, 750
BsmFI	GGGAC	3 cut(s)	322, 466, 860
BsnI	GGCC	8 cut(s)	320, 362, 525, 538, 561, 685, 707, 743
BsoBI	CYCGRG	2 cut(s)	701, 749
Bsp1286I	GDGCHC	2 cut(s)	99, 521
Bsp143I	GATC	1 cut(s)	615
BspANI	GGCC	8 cut(s)	320, 362, 525, 538, 561, 685, 707, 743
BspFNI	CGCG	3 cut(s)	383, 695, 803
BspQI	GCTCTTC	1 cut(s)	123
BspT107I	GGYRCC	1 cut(s)	278
BspTI	CTTAAG	1 cut(s)	122
BsrFI	RCCGGY	2 cut(s)	368, 722
BsrI	ACTGG	4 cut(s)	60, 96, 414, 514
BssAI	RCCGGY	2 cut(s)	368, 722
BssECI	CCNNGG	8 cut(s)	215, 457, 520, 679, 738, 748, 749, 786
BssHII	GCGCGC	1 cut(s)	693
BssMI	GATC	1 cut(s)	615
BssNI	GRCGYC	1 cut(s)	279
BssSI	CACGAG	1 cut(s)	314
BssT1I	CCWWGG	1 cut(s)	679
Bst2BI	CACGAG	1 cut(s)	314
Bst2UI	CCWGG	3 cut(s)	247, 356, 535
Bst6I	CTCTTC	1 cut(s)	123
BstACI	GRCGYC	1 cut(s)	279
BstAFI	CTTAAG	1 cut(s)	122
BstBAI	YACGTR	1 cut(s)	490
BstC8I	GCNNGC	7 cut(s)	302, 370, 563, 695, 718, 793, 837
BstDSI	CCRYGG	1 cut(s)	457
BstENI	CCTNNNNNAGG	1 cut(s)	314
BstF5I	GGATG	2 cut(s)	132, 198
BstFNI	CGCG	3 cut(s)	383, 695, 803
BstH2I	RGCGCY	3 cut(s)	72, 117, 282
BstHHI	GCGC	6 cut(s)	71, 116, 281, 533, 695, 697
BstKTI	GATC	1 cut(s)	618
BstMBI	GATC	1 cut(s)	615
BstMCI	CGRYCG	2 cut(s)	429, 786
BstMWI	GCNNNNNNNGC	6 cut(s)	94, 389, 441, 571, 713, 788
BstNI	CCWGG	3 cut(s)	247, 356, 535
BstNSI	RCATGY	1 cut(s)	867
BstSFI	CTRYAG	1 cut(s)	439
BstSLI	GKGCMC	1 cut(s)	521
BstUI	CGCG	3 cut(s)	383, 695, 803
BstV1I	GCAGC	3 cut(s)	97, 171, 434
BstXI	CCANNNNNNTGG	3 cut(s)	421, 637, 843
BsuI	GTATCC	2 cut(s)	15, 765
BsuRI	GGCC	8 cut(s)	320, 362, 525, 538, 561, 685, 707, 743
BtgI	CCRYGG	1 cut(s)	457
BtrI	CACGTC	1 cut(s)	421
BtsCI	GGATG	2 cut(s)	132, 198
BtsI	GCAGTG	1 cut(s)	24
BtsIMutI	CAGTG	4 cut(s)	24, 89, 421, 521
Cac8I	GCNNGC	7 cut(s)	302, 370, 563, 695, 718, 793, 837
CaiI	CAGNNNCTG	1 cut(s)	91
CfoI	GCGC	6 cut(s)	71, 116, 281, 533, 695, 697
Cfr10I	RCCGGY	2 cut(s)	368, 722
Cfr13I	GGNCC	9 cut(s)	180, 361, 453, 511, 537, 560, 683, 741, 847
Cfr9I	CCCGGG	1 cut(s)	749
CsiI	ACCWGGT	1 cut(s)	354
Csp6I	GTAC	4 cut(s)	103, 154, 268, 673
CviAII	CATG	3 cut(s)	613, 841, 864
CviQI	GTAC	4 cut(s)	103, 154, 268, 673
DinI	GGCGCC	1 cut(s)	280
DpnI	GATC	1 cut(s)	617
DpnII	GATC	1 cut(s)	615
DraIII	CACNNNGTG	1 cut(s)	358
DrdI	GACNNNNNNGTC	1 cut(s)	425
DseDI	GACNNNNNNGTC	1 cut(s)	425
EaeI	YGGCCR	1 cut(s)	523
Eam1104I	CTCTTC	1 cut(s)	123
EarI	CTCTTC	1 cut(s)	123
EciI	GGCGGA	2 cut(s)	226, 561
Eco130I	CCWWGG	1 cut(s)	679
Eco147I	AGGCCT	1 cut(s)	320
Eco47I	GGWCC	4 cut(s)	180, 453, 511, 847
Eco88I	CYCGRG	2 cut(s)	701, 749
EcoNI	CCTNNNNNAGG	1 cut(s)	314
EcoO109I	RGGNCCY	3 cut(s)	453, 741, 847
EcoRII	CCWGG	3 cut(s)	245, 354, 533
EcoT14I	CCWWGG	1 cut(s)	679
EgeI	GGCGCC	1 cut(s)	280
EheI	GGCGCC	1 cut(s)	280
ErhI	CCWWGG	1 cut(s)	679
FaeI	CATG	3 cut(s)	616, 844, 867
FaiI	YATR	5 cut(s)	614, 620, 661, 842, 865
FaqI	GGGAC	3 cut(s)	322, 466, 860
FatI	CATG	3 cut(s)	612, 840, 863
FauI	CCCGC	4 cut(s)	443, 570, 634, 858
FbaI	TGATCA	1 cut(s)	615
FblI	GTMKAC	2 cut(s)	192, 429
Fnu4HI	GCNGC	6 cut(s)	86, 160, 277, 445, 448, 872
FokI	GGATG	2 cut(s)	119, 185
Fsp4HI	GCNGC	6 cut(s)	86, 160, 277, 445, 448, 872
GlaI	GCGC	6 cut(s)	70, 115, 280, 532, 694, 696
GluI	GCNGC	6 cut(s)	86, 160, 277, 445, 448, 872
HaeII	RGCGCY	3 cut(s)	72, 117, 282
HaeIII	GGCC	8 cut(s)	320, 362, 525, 538, 561, 685, 707, 743
HapII	CCGG	9 cut(s)	217, 369, 469, 522, 723, 738, 750, 758, 786
HhaI	GCGC	6 cut(s)	71, 116, 281, 533, 695, 697
Hin1I	GRCGYC	1 cut(s)	279
Hin1II	CATG	3 cut(s)	616, 844, 867
Hin6I	GCGC	6 cut(s)	69, 114, 279, 531, 693, 695
HinP1I	GCGC	6 cut(s)	69, 114, 279, 531, 693, 695
HincII	GTYRAC	3 cut(s)	193, 379, 430
HindII	GTYRAC	3 cut(s)	193, 379, 430
HpaII	CCGG	9 cut(s)	217, 369, 469, 522, 723, 738, 750, 758, 786
HphI	GGTGA	1 cut(s)	536
Hpy166II	GTNNAC	4 cut(s)	193, 379, 430, 814
Hpy188I	TCNGA	4 cut(s)	9, 332, 588, 734
Hpy8I	GTNNAC	4 cut(s)	193, 379, 430, 814
Hpy99I	CGWCG	4 cut(s)	194, 299, 434, 599
HpyAV	CCTTC	1 cut(s)	50
HpyCH4IV	ACGT	4 cut(s)	399, 420, 489, 597
HpyCH4V	TGCA	2 cut(s)	720, 863
HpyF10VI	GCNNNNNNNGC	6 cut(s)	94, 389, 441, 571, 713, 788
HpySE526I	ACGT	4 cut(s)	399, 420, 489, 597
Hsp92I	GRCGYC	1 cut(s)	279
Hsp92II	CATG	3 cut(s)	616, 844, 867
HspAI	GCGC	6 cut(s)	69, 114, 279, 531, 693, 695
KasI	GGCGCC	1 cut(s)	278
KflI	GGGWCCC	2 cut(s)	453, 847
KroI	GCCGGC	1 cut(s)	368
KroNI	GCCGGC	1 cut(s)	370
Ksp22I	TGATCA	1 cut(s)	615
Kzo9I	GATC	1 cut(s)	615
LguI	GCTCTTC	1 cut(s)	123
LmnI	GCTCC	6 cut(s)	42, 82, 217, 467, 589, 644
Lsp1109I	GCAGC	3 cut(s)	97, 171, 434
MabI	ACCWGGT	1 cut(s)	354
MaeII	ACGT	4 cut(s)	399, 420, 489, 597
MaeIII	GTNAC	3 cut(s)	344, 416, 649
MalI	GATC	1 cut(s)	617
MboI	GATC	1 cut(s)	615
MboII	GAAGA	1 cut(s)	110
MhlI	GDGCHC	2 cut(s)	99, 521
MluI	ACGCGT	1 cut(s)	381
Mly113I	GGCGCC	1 cut(s)	279
MmeI	TCCRAC	2 cut(s)	489, 580
MroNI	GCCGGC	1 cut(s)	368
MseI	TTAA	1 cut(s)	123
MspA1I	CMGCKG	1 cut(s)	447
MspCI	CTTAAG	1 cut(s)	122
MspI	CCGG	9 cut(s)	217, 369, 469, 522, 723, 738, 750, 758, 786
MvaI	CCWGG	3 cut(s)	247, 356, 535
MvnI	CGCG	3 cut(s)	383, 695, 803
MwoI	GCNNNNNNNGC	6 cut(s)	94, 389, 441, 571, 713, 788
NaeI	GCCGGC	1 cut(s)	370
NarI	GGCGCC	1 cut(s)	279
NciI	CCSGG	8 cut(s)	217, 470, 522, 739, 750, 751, 759, 787
NdeII	GATC	1 cut(s)	615
NgoMIV	GCCGGC	1 cut(s)	368
NlaIII	CATG	3 cut(s)	616, 844, 867
NmuCI	GTSAC	1 cut(s)	416
NspI	RCATGY	1 cut(s)	867
PaeR7I	CTCGAG	1 cut(s)	701
PauI	GCGCGC	1 cut(s)	693
PceI	AGGCCT	1 cut(s)	320
PciSI	GCTCTTC	1 cut(s)	123
PcsI	WCGNNNNNNNCGW	2 cut(s)	396, 426
PdiI	GCCGGC	1 cut(s)	370
PkrI	GCNGC	6 cut(s)	87, 161, 278, 446, 449, 873
PluTI	GGCGCC	1 cut(s)	282
Ppu21I	YACGTR	1 cut(s)	490
PpuMI	RGGWCCY	2 cut(s)	453, 847
Psp1406I	AACGTT	1 cut(s)	399
Psp5II	RGGWCCY	2 cut(s)	453, 847
Psp6I	CCWGG	3 cut(s)	245, 354, 533
PspGI	CCWGG	3 cut(s)	245, 354, 533
PspPI	GGNCC	9 cut(s)	180, 361, 453, 511, 537, 560, 683, 741, 847
PspPPI	RGGWCCY	2 cut(s)	453, 847
PspXI	VCTCGAGB	1 cut(s)	701
PstNI	CAGNNNCTG	1 cut(s)	91
PteI	GCGCGC	1 cut(s)	693
RsaI	GTAC	4 cut(s)	104, 155, 269, 674
RsaNI	GTAC	4 cut(s)	103, 154, 268, 673
SalI	GTCGAC	2 cut(s)	191, 428
SapI	GCTCTTC	1 cut(s)	123
SaqAI	TTAA	1 cut(s)	123
SatI	GCNGC	6 cut(s)	86, 160, 277, 445, 448, 872
Sau3AI	GATC	1 cut(s)	615
Sau96I	GGNCC	9 cut(s)	180, 361, 453, 511, 537, 560, 683, 741, 847
SduI	GDGCHC	2 cut(s)	99, 521
SexAI	ACCWGGT	1 cut(s)	354
SfcI	CTRYAG	1 cut(s)	439
SfoI	GGCGCC	1 cut(s)	280
Sfr274I	CTCGAG	1 cut(s)	701
SgrAI	CRCCGGYG	1 cut(s)	722
SinI	GGWCC	4 cut(s)	180, 453, 511, 847
SlaI	CTCGAG	1 cut(s)	701
SmaI	CCCGGG	1 cut(s)	751
SmlI	CTYRAG	3 cut(s)	98, 122, 701
SmoI	CTYRAG	3 cut(s)	98, 122, 701
SseBI	AGGCCT	1 cut(s)	320
SspDI	GGCGCC	1 cut(s)	278
StuI	AGGCCT	1 cut(s)	320
StyI	CCWWGG	1 cut(s)	679
TaiI	ACGT	4 cut(s)	402, 423, 492, 600
TaqI	TCGA	3 cut(s)	192, 429, 702
TatI	WGTACW	1 cut(s)	267
TauI	GCSGC	3 cut(s)	279, 447, 874
Tru1I	TTAA	1 cut(s)	123
Tru9I	TTAA	1 cut(s)	123
TscAI	CASTG	4 cut(s)	31, 96, 421, 521
TseFI	GTSAC	1 cut(s)	416
TseI	GCWGC	3 cut(s)	85, 159, 447
Tsp45I	GTSAC	1 cut(s)	416
TspDTI	ATGAA	1 cut(s)	187
TspGWI	ACGGA	1 cut(s)	332
TspMI	CCCGGG	1 cut(s)	749
TspRI	CASTG	4 cut(s)	31, 96, 421, 521
Vha464I	CTTAAG	1 cut(s)	122
VpaK11BI	GGWCC	4 cut(s)	180, 453, 511, 847
XagI	CCTNNNNNAGG	1 cut(s)	314
XceI	RCATGY	1 cut(s)	867
XhoI	CTCGAG	1 cut(s)	701
XmaI	CCCGGG	1 cut(s)	749
XmiI	GTMKAC	2 cut(s)	192, 429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.