pycom10g03530

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002144
Physical Location & Seq
Reverse (-)
3846484 .. 3847365
882 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g03530.1

Sequence Viewer

Length: 882 bp
ATGTATCCTATTTACCACTTTGCCACTTCTCTCTTACTGCTCGTGTTTCTGGCCGAGCCCGGTATGGGTGCTTTAGTGGAGGAGCAGCCCCTGGTGCTCAAGTACCACAACGGCGCTCTTCTCAAGGGCAACATCACCGTCAATCTCATCTGGTACGGCCACTTCACCCCAATCCAACGGTCCATCATCGTCGACTTCATCCACTCCCTCGCTCCTCGGCGCGGCCCACTCCCCTCTGCCTCCTCCTGGTGGAAAACCACCGAGAAGTACAAGGGCGGCGCCTCCAATCTCGTCGTCGGCAGGCAAATCCTCCACGAGGCCTACTCTCTCGGAAAGTCCCTCGGGAACCGGCACCTGGTAGCCCTCGCCGGAAAAGTGAACGCGTTGAAGGCCATCAACGTGGTTTTGACTGCCAGTGACGTGGCGGTCGACGGATTCTGTAGCCGCTGCGGGACGCACGGCTCCGCCCATGACAAGAAGTCGGCTTACGTGTGGGTTGGGAACTCGGTGGTCCAGTGCCCCGGCCAATGCGCCTGGCCCTTTCACCAGCCCATTTACGGCCCGCAAACCCCGCCATTAGTGGCCCCAAACGGCGACGTCGGAGTTGACGGCATGATCATAAACCTCGCCACTCTCTTGGCGGGAACCGTAACCAATCCGTACAACAATGGATACTTCCAAGGGCCGGCCAGCGCGCCGCTCGAGGCGGTCTCTGCCTGCACGGGCATTTTCGGAACCGGGGCATACCCGGGTTATCCGGGTCAAGTCCTGGTGGACAAGGCGACCGGGGCGAGCTACAACGCGGTCGGGGTGAACGGGCGGAGGTTTCTACTGCCAGCCATGTGGGACCCGCAGACCTCTGCATGCAAGCCGCTTGTGTGA

Protein Analysis

294

Amino Acids

31.16

Weight (kDa)

8.97

Isoelectric Point (pI)

23.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 33 - 290 7.4e-118 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AatII GACGTC 1 cut(s) 600
AccB1I GGYRCC 2 cut(s) 278, 351
AccBSI CCGCTC 1 cut(s) 700
AccI GTMKAC 2 cut(s) 192, 429
AccII CGCG 4 cut(s) 222, 383, 695, 803
AcoI YGGCCR 4 cut(s) 51, 157, 523, 687
AcyI GRCGYC 2 cut(s) 279, 597
AfaI GTAC 4 cut(s) 104, 155, 269, 662
AfiI CCNNNNNNNGG 8 cut(s) 65, 221, 246, 249, 316, 355, 557, 685
AflIII ACRYGT 2 cut(s) 381, 489
AgsI TTSAA 1 cut(s) 388
AhdI GACNNNNNGTC 1 cut(s) 478
AjiI CACGTC 1 cut(s) 421
AjnI CCWGG 5 cut(s) 90, 245, 354, 533, 768
AluBI AGCT 1 cut(s) 795
AluI AGCT 1 cut(s) 795
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 1 cut(s) 715
AlwNI CAGNNNCTG 1 cut(s) 91
Ama87I CYCGRG 3 cut(s) 341, 701, 748
ApeKI GCWGC 2 cut(s) 85, 447
AspLEI GCGC 6 cut(s) 116, 222, 281, 533, 695, 697
AspS9I GGNCC 8 cut(s) 180, 224, 511, 537, 560, 583, 683, 847
AsuC2I CCSGG 7 cut(s) 60, 522, 739, 749, 750, 759, 787
AsuHPI GGTGA 4 cut(s) 127, 157, 536, 823
AvaI CYCGRG 3 cut(s) 341, 701, 748
AvaII GGWCC 3 cut(s) 180, 511, 847
BaeGI GKGCMC 1 cut(s) 521
BanI GGYRCC 2 cut(s) 278, 351
BanII GRGCYC 1 cut(s) 60
BarI GAAGNNNNNNTAC 4 cut(s) 146, 178, 470, 502
BauI CACGAG 2 cut(s) 41, 314
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 97, 434
BccI CCATC 2 cut(s) 191, 401
BceAI ACGGC 6 cut(s) 127, 172, 475, 574, 607, 625
BciT130I CCWGG 5 cut(s) 92, 247, 356, 535, 770
BciVI GTATCC 2 cut(s) 15, 665
BclI TGATCA 1 cut(s) 615
BcnI CCSGG 7 cut(s) 60, 522, 739, 749, 750, 759, 787
BcoDI GTCTC 1 cut(s) 715
BfmI CTRYAG 1 cut(s) 439
BfoI RGCGCY 2 cut(s) 117, 282
BfuI GTATCC 2 cut(s) 15, 665
BisI GCNGC 7 cut(s) 86, 223, 277, 445, 448, 698, 872
BlsI GCNGC 7 cut(s) 87, 224, 278, 446, 449, 699, 873
Bme18I GGWCC 3 cut(s) 180, 511, 847
BmeRI GACNNNNNGTC 1 cut(s) 478
BmeT110I CYCGRG 3 cut(s) 341, 701, 748
BmgBI CACGTC 1 cut(s) 421
BmgT120I GGNCC 8 cut(s) 180, 224, 511, 537, 560, 583, 683, 847
BmiI GGNNCC 9 cut(s) 280, 347, 353, 463, 585, 646, 736, 848, 849
BplI GAGNNNNNCTC 4 cut(s) 308, 340, 695, 727
BpuEI CTTGAG 2 cut(s) 83, 107
BpuMI CCSGG 7 cut(s) 60, 522, 739, 749, 750, 759, 787
BsaAI YACGTR 1 cut(s) 490
BsaHI GRCGYC 2 cut(s) 279, 597
BsaI GGTCTC 1 cut(s) 715
BsaJI CCNNGG 8 cut(s) 90, 215, 340, 520, 679, 738, 748, 786
Bsc4I CCNNNNNNNGG 8 cut(s) 65, 221, 246, 249, 316, 355, 557, 685
Bse118I RCCGGY 2 cut(s) 348, 685
Bse1I ACTGG 2 cut(s) 414, 514
BseBI CCWGG 5 cut(s) 92, 247, 356, 535, 770
BseDI CCNNGG 8 cut(s) 90, 215, 340, 520, 679, 738, 748, 786
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 8 cut(s) 65, 221, 246, 249, 316, 355, 557, 685
BseNI ACTGG 2 cut(s) 414, 514
BsePI GCGCGC 1 cut(s) 693
BseRI GAGGAG 3 cut(s) 95, 204, 232
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 2 cut(s) 97, 434
BsgI GTGCAG 1 cut(s) 703
Bsh1236I CGCG 4 cut(s) 222, 383, 695, 803
Bsh1285I CGRYCG 3 cut(s) 429, 786, 807
BshNI GGYRCC 2 cut(s) 278, 351
BsiEI CGRYCG 3 cut(s) 429, 786, 807
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 3 cut(s) 341, 701, 748
BsiSI CCGG 9 cut(s) 60, 349, 369, 522, 686, 738, 749, 758, 786
BslFI GGGAC 3 cut(s) 322, 466, 860
BslI CCNNNNNNNGG 8 cut(s) 65, 221, 246, 249, 316, 355, 557, 685
BsmAI GTCTC 1 cut(s) 715
BsmFI GGGAC 3 cut(s) 322, 466, 860
Bso31I GGTCTC 1 cut(s) 715
BsoBI CYCGRG 3 cut(s) 341, 701, 748
Bsp1286I GDGCHC 3 cut(s) 60, 99, 521
Bsp143I GATC 1 cut(s) 615
BspFNI CGCG 4 cut(s) 222, 383, 695, 803
BspLI GGNNCC 9 cut(s) 280, 347, 353, 463, 585, 646, 736, 848, 849
BspQI GCTCTTC 1 cut(s) 123
BspT107I GGYRCC 2 cut(s) 278, 351
BspTNI GGTCTC 1 cut(s) 715
BsrBI CCGCTC 1 cut(s) 700
BsrFI RCCGGY 2 cut(s) 348, 685
BsrI ACTGG 2 cut(s) 414, 514
BssAI RCCGGY 2 cut(s) 348, 685
BssECI CCNNGG 8 cut(s) 90, 215, 340, 520, 679, 738, 748, 786
BssHII GCGCGC 1 cut(s) 693
BssMI GATC 1 cut(s) 615
BssNI GRCGYC 2 cut(s) 279, 597
BssSI CACGAG 2 cut(s) 41, 314
BssT1I CCWWGG 1 cut(s) 679
Bst2BI CACGAG 2 cut(s) 41, 314
Bst2UI CCWGG 5 cut(s) 92, 247, 356, 535, 770
Bst4CI ACNGT 3 cut(s) 139, 180, 649
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 2 cut(s) 279, 597
BstBAI YACGTR 1 cut(s) 490
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 4 cut(s) 222, 383, 695, 803
BstH2I RGCGCY 2 cut(s) 117, 282
BstHHI GCGC 6 cut(s) 116, 222, 281, 533, 695, 697
BstKTI GATC 1 cut(s) 618
BstMAI GTCTC 1 cut(s) 715
BstMBI GATC 1 cut(s) 615
BstMCI CGRYCG 3 cut(s) 429, 786, 807
BstMWI GCNNNNNNNGC 5 cut(s) 94, 389, 571, 713, 788
BstNI CCWGG 5 cut(s) 92, 247, 356, 535, 770
BstNSI RCATGY 1 cut(s) 867
BstSFI CTRYAG 1 cut(s) 439
BstSLI GKGCMC 1 cut(s) 521
BstUI CGCG 4 cut(s) 222, 383, 695, 803
BstV1I GCAGC 2 cut(s) 97, 434
BstXI CCANNNNNNTGG 4 cut(s) 400, 421, 637, 843
BsuI GTATCC 2 cut(s) 15, 665
BtrI CACGTC 1 cut(s) 421
BtsCI GGATG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 421, 521
CaiI CAGNNNCTG 1 cut(s) 91
CfoI GCGC 6 cut(s) 116, 222, 281, 533, 695, 697
Cfr10I RCCGGY 2 cut(s) 348, 685
Cfr13I GGNCC 8 cut(s) 180, 224, 511, 537, 560, 583, 683, 847
Cfr9I CCCGGG 1 cut(s) 748
CseI GACGC 1 cut(s) 463
CsiI ACCWGGT 1 cut(s) 354
Csp6I GTAC 4 cut(s) 103, 154, 268, 661
CviAII CATG 4 cut(s) 470, 613, 841, 864
CviQI GTAC 4 cut(s) 103, 154, 268, 661
DinI GGCGCC 1 cut(s) 280
DpnI GATC 1 cut(s) 617
DpnII GATC 1 cut(s) 615
DrdI GACNNNNNNGTC 1 cut(s) 425
DriI GACNNNNNGTC 1 cut(s) 478
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 4 cut(s) 51, 157, 523, 687
Eam1104I CTCTTC 1 cut(s) 123
Eam1105I GACNNNNNGTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 2 cut(s) 454, 835
Eco130I CCWWGG 1 cut(s) 679
Eco147I AGGCCT 1 cut(s) 320
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 715
Eco47I GGWCC 3 cut(s) 180, 511, 847
Eco88I CYCGRG 3 cut(s) 341, 701, 748
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 847
EcoRII CCWGG 5 cut(s) 90, 245, 354, 533, 768
EcoT14I CCWWGG 1 cut(s) 679
EcoT38I GRGCYC 1 cut(s) 60
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 679
FaeI CATG 4 cut(s) 473, 616, 844, 867
FaiI YATR 7 cut(s) 65, 471, 614, 620, 745, 842, 865
FaqI GGGAC 3 cut(s) 322, 466, 860
FatI CATG 4 cut(s) 469, 612, 840, 863
FauI CCCGC 5 cut(s) 443, 570, 579, 634, 858
FbaI TGATCA 1 cut(s) 615
FblI GTMKAC 2 cut(s) 192, 429
Fnu4HI GCNGC 7 cut(s) 86, 223, 277, 445, 448, 698, 872
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 1 cut(s) 60
FseI GGCCGGCC 1 cut(s) 689
Fsp4HI GCNGC 7 cut(s) 86, 223, 277, 445, 448, 698, 872
GlaI GCGC 6 cut(s) 115, 221, 280, 532, 694, 696
GluI GCNGC 7 cut(s) 86, 223, 277, 445, 448, 698, 872
HaeII RGCGCY 2 cut(s) 117, 282
HapII CCGG 9 cut(s) 60, 349, 369, 522, 686, 738, 749, 758, 786
HgaI GACGC 1 cut(s) 463
HhaI GCGC 6 cut(s) 116, 222, 281, 533, 695, 697
Hin1I GRCGYC 2 cut(s) 279, 597
Hin1II CATG 4 cut(s) 473, 616, 844, 867
Hin6I GCGC 6 cut(s) 114, 220, 279, 531, 693, 695
HinP1I GCGC 6 cut(s) 114, 220, 279, 531, 693, 695
HincII GTYRAC 3 cut(s) 193, 430, 607
HindII GTYRAC 3 cut(s) 193, 430, 607
HinfI GANTC 1 cut(s) 435
HpaII CCGG 9 cut(s) 60, 349, 369, 522, 686, 738, 749, 758, 786
HphI GGTGA 4 cut(s) 127, 157, 536, 823
Hpy166II GTNNAC 6 cut(s) 193, 379, 430, 607, 775, 814
Hpy188I TCNGA 3 cut(s) 332, 602, 734
Hpy188III TCNNGA 1 cut(s) 343
Hpy8I GTNNAC 6 cut(s) 193, 379, 430, 607, 775, 814
Hpy99I CGWCG 6 cut(s) 194, 296, 299, 434, 599, 602
HpyAV CCTTC 1 cut(s) 382
HpyCH4III ACNGT 3 cut(s) 139, 180, 649
HpyCH4IV ACGT 4 cut(s) 399, 420, 489, 597
HpyCH4V TGCA 3 cut(s) 720, 863, 867
HpyF10VI GCNNNNNNNGC 5 cut(s) 94, 389, 571, 713, 788
HpySE526I ACGT 4 cut(s) 399, 420, 489, 597
Hsp92I GRCGYC 2 cut(s) 279, 597
Hsp92II CATG 4 cut(s) 473, 616, 844, 867
HspAI GCGC 6 cut(s) 114, 220, 279, 531, 693, 695
KasI GGCGCC 1 cut(s) 278
KflI GGGWCCC 1 cut(s) 847
KroI GCCGGC 1 cut(s) 685
KroNI GCCGGC 1 cut(s) 687
Ksp22I TGATCA 1 cut(s) 615
Kzo9I GATC 1 cut(s) 615
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 3 cut(s) 82, 217, 467
Lsp1109I GCAGC 2 cut(s) 97, 434
MabI ACCWGGT 1 cut(s) 354
MaeII ACGT 4 cut(s) 399, 420, 489, 597
MaeIII GTNAC 2 cut(s) 416, 649
MalI GATC 1 cut(s) 617
MbiI CCGCTC 1 cut(s) 700
MboI GATC 1 cut(s) 615
MboII GAAGA 1 cut(s) 110
MhlI GDGCHC 3 cut(s) 60, 99, 521
MluI ACGCGT 1 cut(s) 381
Mly113I GGCGCC 1 cut(s) 279
MmeI TCCRAC 2 cut(s) 199, 580
MroNI GCCGGC 1 cut(s) 685
MslI CAYNNNNRTG 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 447
MspI CCGG 9 cut(s) 60, 349, 369, 522, 686, 738, 749, 758, 786
MvaI CCWGG 5 cut(s) 92, 247, 356, 535, 770
MvnI CGCG 4 cut(s) 222, 383, 695, 803
MwoI GCNNNNNNNGC 5 cut(s) 94, 389, 571, 713, 788
NaeI GCCGGC 1 cut(s) 687
NarI GGCGCC 1 cut(s) 279
NciI CCSGG 7 cut(s) 60, 522, 739, 749, 750, 759, 787
NdeII GATC 1 cut(s) 615
NgoMIV GCCGGC 1 cut(s) 685
NlaIII CATG 4 cut(s) 473, 616, 844, 867
NlaIV GGNNCC 9 cut(s) 280, 347, 353, 463, 585, 646, 736, 848, 849
NmeAIII GCCGAG 2 cut(s) 79, 196
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 1 cut(s) 867
PaeI GCATGC 1 cut(s) 867
PaeR7I CTCGAG 1 cut(s) 701
PauI GCGCGC 1 cut(s) 693
PceI AGGCCT 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PdiI GCCGGC 1 cut(s) 687
PfeI GAWTC 1 cut(s) 435
PkrI GCNGC 7 cut(s) 87, 224, 278, 446, 449, 699, 873
PluTI GGCGCC 1 cut(s) 282
Ppu21I YACGTR 1 cut(s) 490
PpuMI RGGWCCY 1 cut(s) 847
Psp5II RGGWCCY 1 cut(s) 847
Psp6I CCWGG 5 cut(s) 90, 245, 354, 533, 768
PspGI CCWGG 5 cut(s) 90, 245, 354, 533, 768
PspN4I GGNNCC 9 cut(s) 280, 347, 353, 463, 585, 646, 736, 848, 849
PspPI GGNCC 8 cut(s) 180, 224, 511, 537, 560, 583, 683, 847
PspPPI RGGWCCY 1 cut(s) 847
PspXI VCTCGAGB 1 cut(s) 701
PstNI CAGNNNCTG 1 cut(s) 91
PteI GCGCGC 1 cut(s) 693
RigI GGCCGGCC 1 cut(s) 689
RsaI GTAC 4 cut(s) 104, 155, 269, 662
RsaNI GTAC 4 cut(s) 103, 154, 268, 661
RseI CAYNNNNRTG 1 cut(s) 398
SalI GTCGAC 2 cut(s) 191, 428
SapI GCTCTTC 1 cut(s) 123
SatI GCNGC 7 cut(s) 86, 223, 277, 445, 448, 698, 872
Sau3AI GATC 1 cut(s) 615
Sau96I GGNCC 8 cut(s) 180, 224, 511, 537, 560, 583, 683, 847
SduI GDGCHC 3 cut(s) 60, 99, 521
SetI ASST 9 cut(s) 357, 402, 423, 492, 600, 627, 797, 827, 860
SexAI ACCWGGT 1 cut(s) 354
SfcI CTRYAG 1 cut(s) 439
SfoI GGCGCC 1 cut(s) 280
Sfr274I CTCGAG 1 cut(s) 701
SinI GGWCC 3 cut(s) 180, 511, 847
SlaI CTCGAG 1 cut(s) 701
SmaI CCCGGG 1 cut(s) 750
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 3 cut(s) 98, 122, 701
SmoI CTYRAG 3 cut(s) 98, 122, 701
SphI GCATGC 1 cut(s) 867
SseBI AGGCCT 1 cut(s) 320
SspDI GGCGCC 1 cut(s) 278
StuI AGGCCT 1 cut(s) 320
StyI CCWWGG 1 cut(s) 679
TaaI ACNGT 3 cut(s) 139, 180, 649
TaiI ACGT 4 cut(s) 402, 423, 492, 600
TaqI TCGA 3 cut(s) 192, 429, 702
TatI WGTACW 1 cut(s) 267
TauI GCSGC 5 cut(s) 225, 279, 447, 700, 874
TfiI GAWTC 1 cut(s) 435
TscAI CASTG 2 cut(s) 421, 521
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 2 cut(s) 85, 447
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 1 cut(s) 187
TspGWI ACGGA 2 cut(s) 447, 648
TspMI CCCGGG 1 cut(s) 748
TspRI CASTG 2 cut(s) 421, 521
VpaK11BI GGWCC 3 cut(s) 180, 511, 847
XagI CCTNNNNNAGG 1 cut(s) 314
XceI RCATGY 1 cut(s) 867
XhoI CTCGAG 1 cut(s) 701
XmaI CCCGGG 1 cut(s) 748
XmiI GTMKAC 2 cut(s) 192, 429
ZraI GACGTC 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.