Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006294

Physical Location & Seq

Genomic Coordinates Reverse (-)

26431376 .. 26433900

Gene Structure 2525 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G66120.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGTTTAGGCATGTTCACGGAAAGAGAGTGCTTGTGGTCACCAATGATCGAGTTGCTCCTCTCTACCTGGACAAGACTATAGATGCTTTAACCAGAGGAAATCCAAACGTGACAGTGGAAAGTGTAATTTTGCCTGATGGAGAGAAATACAAAGACATGGATACTCTCATGAAAGTCTTCGACAAGGCCATTGAGTCTCGCCTAGATAGACGATGTACATTTGTTGCCCTTGGAGGCGGTGTGATAGGCGATATGTGTGGATATGCTGCTGCATCGTACCTACGTGGTGTCAATTTCATCCAAATTCCTACCACTGTTATGGCACAGGTTGATTCTTCTGTAGGTGGCAAAACAGGCATAAATCATCGTCTTGGAAAGAATTTGATCGGTGCATTTTACCAACCACAGTGTGTGCTAGTAGACACTGACACGTTGAACACATTGCCAGATAGGGAGATGGCATCAGGCCTAGCTGAAGTGATCAAGTACGGGCTTATCAGGGATGCCGAGTTCTTTGAATGGCAGGAGAAGAACATTGAGGCATTGCTGGCAAGGGATCCAGCTGCATTAGCTTTTGCTATAAAGCGATCATGTGAAAACAAGGCAGACGTTGTATCACAAGATGAGAAAGAAAGCGGCTTGCGAGCAACCTTAAATTTGGGTCATACCTTTGGCCATGCCATAGAAACTGGCTTTGGTTATGGAGAGTGGCTCCATGGAGAAGCTGTTGCAGCTGGCACGGTAATGGCAGTGGACATGTCATATCGTCTTGGTTGGATCGATGAATCGATTGTTAAGAGAGTGAACAAGATCCTAGTACGGGCGAAATTGCCTACAACTCCGCCAGAGAGCATGACCGTCAGCATGTTCAAGTCCATAATGGCGGTGGACAAGAAAGTGGCAGATGGACTGCTGAGGCTGATTCTTCTCAAAGGCCCGCTGGGAAACTGCGTGTTCACTGGAGATTATGATCGGGAAGCGTTGGATGCCACGCTTCGTGCATTCTCCAAATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003856 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0012505 GO:0016053 GO:0016829 GO:0016835 GO:0016838 GO:0019632 GO:0019752 GO:0031410 GO:0031982 GO:0031984 GO:0032787 GO:0033587 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:0072330 GO:0097708 GO:0098791 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

DHQ_synthase

Protein Analysis

338

Amino Acids

37.08

Weight (kDa)

6.61

Isoelectric Point (pI)

29.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fe-ADH_2	PF13685	94 - 251	1.4e-07	Iron-containing alcohol dehydrogenase
DHQ_synthase	PF01761	146 - 258	1.6e-48	3-dehydroquinate synthase N-terminal
DHQS_C	PF24621	260 - 404	1.1e-51	3-dehydroquinate synthase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66120 AT5G66120
fragaria_vesca	FvH4_1g11430
malus_domestica	MD15G1242600.v1.1
prunus_persica	Prupe.7G171600_v2.0.a1
pyrus_communis	pycom02g09850 pycom15g21380
rosa_chinensis	RchiOBHm_Chr2g0099011 RchiOBHm_Chr2g0099021
rosa_laevigata	RLG00000016840
rosa_multiflora	Rmu_co8359771.1_g000001 Rmu_sc0000577.1_g000012 Rmu_sc0009422.1_g000001
rosa_roxburghii	Rroxscaffold_2G00143490 Rroxscaffold_2G00143510
rosa_rugosa	Rorug02G0075600 Rorug02G0075700
rosa_samantha	Rh2AG125000 Rh2AG125100 Rh2BG128200 Rh2BG128300 Rh2CG129200 Rh2CG129300 Rh2DG129400 Rh2DG129500
rosa_wichuraiana	Rw2G009610 Rw2G009620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	420
AciI	CCGC	5 cut(s)	237, 636, 842, 884, 938
AclWI	GGATC	4 cut(s)	551, 564, 785, 805
AcoI	YGGCCR	1 cut(s)	673
AcsI	RAATTY	3 cut(s)	303, 379, 655
AcuI	CTGAAG	1 cut(s)	495
AdeI	CACNNNGTG	1 cut(s)	410
AfaI	GTAC	4 cut(s)	217, 278, 488, 819
AfiI	CCNNNNNNNGG	1 cut(s)	820
AflIII	ACRYGT	2 cut(s)	429, 756
AgsI	TTSAA	3 cut(s)	436, 518, 871
AjnI	CCWGG	1 cut(s)	66
AjuI	GAANNNNNNNTTGG	2 cut(s)	678, 710
AloI	GAACNNNNNNTCC	2 cut(s)	494, 526
AluBI	AGCT	5 cut(s)	473, 563, 572, 725, 734
AluI	AGCT	5 cut(s)	473, 563, 572, 725, 734
Alw26I	GTCTC	1 cut(s)	201
AlwI	GGATC	4 cut(s)	551, 564, 785, 805
AoxI	GGCC	4 cut(s)	186, 466, 673, 934
ApeKI	GCWGC	4 cut(s)	266, 269, 563, 731
ApoI	RAATTY	3 cut(s)	303, 379, 655
Asp700I	GAANNNNTTC	1 cut(s)	176
AspS9I	GGNCC	1 cut(s)	935
AsuHPI	GGTGA	1 cut(s)	31
BalI	TGGCCA	1 cut(s)	675
BamHI	GGATCC	1 cut(s)	556
BbsI	GAAGAC	1 cut(s)	169
BbvCI	CCTCAGC	1 cut(s)	914
BbvI	GCAGC	4 cut(s)	253, 256, 550, 743
BccI	CCATC	3 cut(s)	131, 451, 899
BciT130I	CCWGG	1 cut(s)	68
BciVI	GTATCC	1 cut(s)	154
BclI	TGATCA	1 cut(s)	480
BcoDI	GTCTC	1 cut(s)	201
BfaI	CTAG	4 cut(s)	203, 416, 470, 815
BfmI	CTRYAG	2 cut(s)	78, 339
BfuI	GTATCC	1 cut(s)	154
BisI	GCNGC	5 cut(s)	267, 270, 564, 637, 732
BlsI	GCNGC	5 cut(s)	268, 271, 565, 638, 733
Bme1390I	CCNGG	1 cut(s)	68
BmgT120I	GGNCC	1 cut(s)	935
BmiI	GGNNCC	2 cut(s)	558, 713
BmrFI	CCNGG	1 cut(s)	68
BmsI	GCATC	5 cut(s)	73, 281, 470, 493, 976
BpiI	GAAGAC	1 cut(s)	169
BplI	GAGNNNNNCTC	2 cut(s)	696, 728
BpmI	CTGGAG	1 cut(s)	981
Bpu10I	CCTNAGC	1 cut(s)	914
Bsa29I	ATCGAT	2 cut(s)	780, 788
BsaAI	YACGTR	1 cut(s)	284
BsaBI	GATNNNNATC	1 cut(s)	969
BsaJI	CCNNGG	2 cut(s)	229, 715
BsaXI	ACNNNNNCTCC	4 cut(s)	225, 255, 711, 741
Bsc4I	CCNNNNNNNGG	1 cut(s)	820
Bse1I	ACTGG	2 cut(s)	694, 964
Bse3DI	GCAATG	2 cut(s)	440, 542
Bse8I	GATNNNNATC	1 cut(s)	969
BseBI	CCWGG	1 cut(s)	68
BseCI	ATCGAT	2 cut(s)	780, 788
BseDI	CCNNGG	2 cut(s)	229, 715
BseGI	GGATG	3 cut(s)	297, 508, 991
BseJI	GATNNNNATC	1 cut(s)	969
BseLI	CCNNNNNNNGG	1 cut(s)	820
BseMI	GCAATG	2 cut(s)	440, 542
BseMII	CTCAG	1 cut(s)	905
BseNI	ACTGG	2 cut(s)	694, 964
BseRI	GAGGAG	1 cut(s)	48
BseXI	GCAGC	4 cut(s)	253, 256, 550, 743
BseYI	CCCAGC	1 cut(s)	940
BshFI	GGCC	4 cut(s)	188, 468, 675, 936
BshVI	ATCGAT	2 cut(s)	780, 788
BslI	CCNNNNNNNGG	1 cut(s)	820
BsmAI	GTCTC	1 cut(s)	201
BsmI	GAATGC	1 cut(s)	1001
BsnI	GGCC	4 cut(s)	188, 468, 675, 936
Bsp1407I	TGTACA	1 cut(s)	215
Bsp143I	GATC	8 cut(s)	46, 384, 480, 556, 587, 777, 810, 970
Bsp19I	CCATGG	1 cut(s)	715
BspACI	CCGC	5 cut(s)	237, 636, 842, 884, 938
BspANI	GGCC	4 cut(s)	188, 468, 675, 936
BspCNI	CTCAG	1 cut(s)	906
BspDI	ATCGAT	2 cut(s)	780, 788
BspHI	TCATGA	1 cut(s)	168
BspLI	GGNNCC	2 cut(s)	558, 713
BspPI	GGATC	4 cut(s)	551, 564, 785, 805
BsrDI	GCAATG	2 cut(s)	440, 542
BsrGI	TGTACA	1 cut(s)	215
BsrI	ACTGG	2 cut(s)	694, 964
BssECI	CCNNGG	2 cut(s)	229, 715
BssMI	GATC	8 cut(s)	46, 384, 480, 556, 587, 777, 810, 970
BssT1I	CCWWGG	2 cut(s)	229, 715
Bst2UI	CCWGG	1 cut(s)	68
Bst4CI	ACNGT	5 cut(s)	115, 316, 408, 742, 859
BstAUI	TGTACA	1 cut(s)	215
BstBAI	YACGTR	1 cut(s)	284
BstC8I	GCNNGC	5 cut(s)	549, 641, 645, 736, 938
BstDEI	CTNAG	1 cut(s)	914
BstDSI	CCRYGG	1 cut(s)	715
BstEII	GGTNACC	1 cut(s)	37
BstF5I	GGATG	3 cut(s)	297, 508, 991
BstKTI	GATC	8 cut(s)	49, 387, 483, 559, 590, 780, 813, 973
BstMAI	GTCTC	1 cut(s)	201
BstMBI	GATC	8 cut(s)	46, 384, 480, 556, 587, 777, 810, 970
BstMWI	GCNNNNNNNGC	5 cut(s)	354, 548, 569, 731, 986
BstNI	CCWGG	1 cut(s)	68
BstNSI	RCATGY	3 cut(s)	14, 760, 868
BstPI	GGTNACC	1 cut(s)	37
BstSCI	CCNGG	1 cut(s)	66
BstSFI	CTRYAG	2 cut(s)	78, 339
BstV1I	GCAGC	4 cut(s)	253, 256, 550, 743
BstV2I	GAAGAC	1 cut(s)	169
BstX2I	RGATCY	2 cut(s)	556, 810
BstXI	CCANNNNNNTGG	1 cut(s)	319
BstYI	RGATCY	2 cut(s)	556, 810
Bsu15I	ATCGAT	2 cut(s)	780, 788
BsuI	GTATCC	1 cut(s)	154
BsuRI	GGCC	4 cut(s)	188, 468, 675, 936
BsuTUI	ATCGAT	2 cut(s)	780, 788
BtgI	CCRYGG	1 cut(s)	715
BtsCI	GGATG	3 cut(s)	297, 508, 991
BtsI	GCAGTG	1 cut(s)	756
BtsIMutI	CAGTG	6 cut(s)	120, 312, 413, 423, 756, 957
Cac8I	GCNNGC	5 cut(s)	549, 641, 645, 736, 938
CciI	TCATGA	1 cut(s)	168
Cfr13I	GGNCC	1 cut(s)	935
ClaI	ATCGAT	2 cut(s)	780, 788
Csp6I	GTAC	4 cut(s)	216, 277, 487, 818
CviAII	CATG	9 cut(s)	11, 157, 169, 591, 677, 716, 757, 853, 865
CviQI	GTAC	4 cut(s)	216, 277, 487, 818
DdeI	CTNAG	1 cut(s)	914
DpnI	GATC	8 cut(s)	48, 386, 482, 558, 589, 779, 812, 972
DpnII	GATC	8 cut(s)	46, 384, 480, 556, 587, 777, 810, 970
DraIII	CACNNNGTG	1 cut(s)	410
EaeI	YGGCCR	1 cut(s)	673
EciI	GGCGGA	1 cut(s)	831
Eco130I	CCWWGG	2 cut(s)	229, 715
Eco147I	AGGCCT	1 cut(s)	468
Eco57I	CTGAAG	1 cut(s)	495
Eco91I	GGTNACC	1 cut(s)	37
EcoO65I	GGTNACC	1 cut(s)	37
EcoRII	CCWGG	1 cut(s)	66
EcoT14I	CCWWGG	2 cut(s)	229, 715
ErhI	CCWWGG	2 cut(s)	229, 715
FaeI	CATG	9 cut(s)	14, 160, 172, 594, 680, 719, 760, 856, 868
FatI	CATG	9 cut(s)	10, 156, 168, 590, 676, 715, 756, 852, 864
FauI	CCCGC	1 cut(s)	945
FbaI	TGATCA	1 cut(s)	480
FblI	GTMKAC	1 cut(s)	420
Fnu4HI	GCNGC	5 cut(s)	267, 270, 564, 637, 732
FokI	GGATG	3 cut(s)	284, 515, 998
Fsp4HI	GCNGC	5 cut(s)	267, 270, 564, 637, 732
FspBI	CTAG	4 cut(s)	203, 416, 470, 815
GluI	GCNGC	5 cut(s)	267, 270, 564, 637, 732
GsaI	CCCAGC	1 cut(s)	944
GsuI	CTGGAG	1 cut(s)	981
HaeIII	GGCC	4 cut(s)	188, 468, 675, 936
Hin1II	CATG	9 cut(s)	14, 160, 172, 594, 680, 719, 760, 856, 868
HinfI	GANTC	4 cut(s)	194, 332, 785, 922
HphI	GGTGA	1 cut(s)	31
Hpy166II	GTNNAC	6 cut(s)	16, 421, 754, 805, 889, 957
Hpy188III	TCNNGA	3 cut(s)	169, 974, 1014
Hpy8I	GTNNAC	6 cut(s)	16, 421, 754, 805, 889, 957
HpyCH4III	ACNGT	5 cut(s)	115, 316, 408, 742, 859
HpyCH4IV	ACGT	4 cut(s)	108, 283, 431, 609
HpyCH4V	TGCA	5 cut(s)	272, 392, 566, 731, 1001
HpyF10VI	GCNNNNNNNGC	5 cut(s)	354, 548, 569, 731, 986
HpyF3I	CTNAG	1 cut(s)	914
HpySE526I	ACGT	4 cut(s)	108, 283, 431, 609
Hsp92II	CATG	9 cut(s)	14, 160, 172, 594, 680, 719, 760, 856, 868
Ksp22I	TGATCA	1 cut(s)	480
Kzo9I	GATC	8 cut(s)	46, 384, 480, 556, 587, 777, 810, 970
LmnI	GCTCC	2 cut(s)	61, 717
Lsp1109I	GCAGC	4 cut(s)	253, 256, 550, 743
LweI	GCATC	5 cut(s)	73, 281, 470, 493, 976
MaeI	CTAG	4 cut(s)	203, 416, 470, 815
MaeII	ACGT	4 cut(s)	108, 283, 431, 609
MaeIII	GTNAC	2 cut(s)	37, 109
MalI	GATC	8 cut(s)	48, 386, 482, 558, 589, 779, 812, 972
MboI	GATC	8 cut(s)	46, 384, 480, 556, 587, 777, 810, 970
MboII	GAAGA	4 cut(s)	169, 327, 541, 917
MflI	RGATCY	2 cut(s)	556, 810
MlsI	TGGCCA	1 cut(s)	675
MluCI	AATT	6 cut(s)	126, 292, 303, 379, 655, 827
MluNI	TGGCCA	1 cut(s)	675
MlyI	GAGTC	1 cut(s)	203
MmeI	TCCRAC	2 cut(s)	755, 963
MnlI	CCTC	5 cut(s)	69, 89, 227, 532, 909
Mox20I	TGGCCA	1 cut(s)	675
MroXI	GAANNNNTTC	1 cut(s)	176
MscI	TGGCCA	1 cut(s)	675
MseI	TTAA	3 cut(s)	89, 653, 795
MslI	CAYNNNNRTG	2 cut(s)	317, 743
Msp20I	TGGCCA	1 cut(s)	675
MspA1I	CMGCKG	3 cut(s)	563, 734, 940
MspR9I	CCNGG	1 cut(s)	68
Mva1269I	GAATGC	1 cut(s)	1001
MvaI	CCWGG	1 cut(s)	68
MwoI	GCNNNNNNNGC	5 cut(s)	354, 548, 569, 731, 986
NcoI	CCATGG	1 cut(s)	715
NdeII	GATC	8 cut(s)	46, 384, 480, 556, 587, 777, 810, 970
NlaIII	CATG	9 cut(s)	14, 160, 172, 594, 680, 719, 760, 856, 868
NlaIV	GGNNCC	2 cut(s)	558, 713
NmeAIII	GCCGAG	1 cut(s)	532
NmuCI	GTSAC	2 cut(s)	37, 109
NspI	RCATGY	3 cut(s)	14, 760, 868
PagI	TCATGA	1 cut(s)	168
PceI	AGGCCT	1 cut(s)	468
PciI	ACATGT	1 cut(s)	756
PctI	GAATGC	1 cut(s)	1001
PdmI	GAANNNNTTC	1 cut(s)	176
PfeI	GAWTC	3 cut(s)	332, 785, 922
PkrI	GCNGC	5 cut(s)	268, 271, 565, 638, 733
PleI	GAGTC	1 cut(s)	202
PpsI	GAGTC	1 cut(s)	202
Ppu21I	YACGTR	1 cut(s)	284
PscI	ACATGT	1 cut(s)	756
Psp6I	CCWGG	1 cut(s)	66
PspEI	GGTNACC	1 cut(s)	37
PspFI	CCCAGC	1 cut(s)	940
PspGI	CCWGG	1 cut(s)	66
PspN4I	GGNNCC	2 cut(s)	558, 713
PspPI	GGNCC	1 cut(s)	935
PsuI	RGATCY	2 cut(s)	556, 810
PvuII	CAGCTG	2 cut(s)	563, 734
RsaI	GTAC	4 cut(s)	217, 278, 488, 819
RsaNI	GTAC	4 cut(s)	216, 277, 487, 818
RseI	CAYNNNNRTG	2 cut(s)	317, 743
SaqAI	TTAA	3 cut(s)	89, 653, 795
SatI	GCNGC	5 cut(s)	267, 270, 564, 637, 732
Sau3AI	GATC	8 cut(s)	46, 384, 480, 556, 587, 777, 810, 970
Sau96I	GGNCC	1 cut(s)	935
SchI	GAGTC	1 cut(s)	203
ScrFI	CCNGG	1 cut(s)	68
SfaNI	GCATC	5 cut(s)	73, 281, 470, 493, 976
SfcI	CTRYAG	2 cut(s)	78, 339
SmiMI	CAYNNNNRTG	2 cut(s)	317, 743
Sse9I	AATT	6 cut(s)	126, 292, 303, 379, 655, 827
SseBI	AGGCCT	1 cut(s)	468
SsiI	CCGC	5 cut(s)	237, 636, 842, 884, 938
SspMI	CTAG	4 cut(s)	203, 416, 470, 815
StuI	AGGCCT	1 cut(s)	468
StyD4I	CCNGG	1 cut(s)	66
StyI	CCWWGG	2 cut(s)	229, 715
TaaI	ACNGT	5 cut(s)	115, 316, 408, 742, 859
TaiI	ACGT	4 cut(s)	111, 286, 434, 612
TaqI	TCGA	4 cut(s)	49, 180, 780, 788
TasI	AATT	6 cut(s)	126, 292, 303, 379, 655, 827
TatI	WGTACW	1 cut(s)	215
TauI	GCSGC	1 cut(s)	639
TfiI	GAWTC	3 cut(s)	332, 785, 922
Tru1I	TTAA	3 cut(s)	89, 653, 795
Tru9I	TTAA	3 cut(s)	89, 653, 795
TscAI	CASTG	6 cut(s)	120, 319, 413, 430, 756, 964
TseFI	GTSAC	2 cut(s)	37, 109
TseI	GCWGC	4 cut(s)	266, 269, 563, 731
Tsp45I	GTSAC	2 cut(s)	37, 109
TspDTI	ATGAA	3 cut(s)	185, 286, 798
TspGWI	ACGGA	1 cut(s)	33
TspRI	CASTG	6 cut(s)	120, 319, 413, 430, 756, 964
XapI	RAATTY	3 cut(s)	303, 379, 655
XceI	RCATGY	3 cut(s)	14, 760, 868
XcmI	CCANNNNNNNNNTGG	1 cut(s)	883
XmiI	GTMKAC	1 cut(s)	420
XmnI	GAANNNNTTC	1 cut(s)	176
XspI	CTAG	4 cut(s)	203, 416, 470, 815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.