Rh2BG128200

3-dehydroquinate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006294
Physical Location & Seq
Reverse (-)
10946606 .. 10961918
15313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG128200.1

Sequence Viewer

Length: 744 bp
ATGAAAGTGTTTGACAAGGCTATTGAGTCGAGACTTGACCGGCGATGTACATTTGTTACCCTTGGAGGTGGTGTGATTGGTGACACGTGTGACTTTGCCGCTGCTTCTTTCCTTCGCGGTGTTAATTTCATTCAGATTCCGACGACTGTTATGGCACAGGAGAGGAATATGGATGCATTAATATCAAGGGATCCAAATGCTCTGGCTTATGCTATAAAGCGCTCATGTGAAATGAAGGCTGAGATTGTATCCTTGGATGAGCTGGAAGGTGGACTGAGGGCCATTCTTAATTTTGGTCATACGTTTGGCCATGCAATAGAAACCGCGGTTGGCTATGGGAAGTGGTTCCATGGAGAAGCTGTTGCAGCTGGCATGGTTATGGCTGTTAATATGTCCTACCGCCTTGGTTGGATTGATGAGTCACTTGCCAAGCGAGTTGACAACATTGTGGAACAGGCCAACTTACCTATTGGCCCTCCTGAATCCATGACTCTTGAGACGTTCAGCAACACCTTCCCCACCGATTACGTGCCTACTGTTTTCGACAATTTTAGTGCAAACGTGGTTGTCAATGGGAGCACTGTTAACCTTGGGTTGTGGGATACAGCTGGACAGGAGGATTATAATAGATTAAGACCCTTGAGTTATCGTGGGGCTGATGTTTTTATTCTGGCATTCTCCCTGATTAGCAAGGCCAGCTATGAAAATGTGTCCAAGAAGGTGAGGATGTTCTCTGTAGTATGA

Protein Analysis

247

Amino Acids

27.17

Weight (kDa)

5.29

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHQ_synthase PF01761 2 - 53 1.4e-15 3-dehydroquinate synthase N-terminal
DHQS_C PF24621 52 - 172 2.2e-38 3-dehydroquinate synthase C-terminal
Ras PF00071 163 - 241 5.2e-21 Ras family
Roc PF08477 166 - 241 3e-08 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AccII CGCG 2 cut(s) 117, 326
AciI CCGC 5 cut(s) 99, 117, 324, 326, 400
AclWI GGATC 2 cut(s) 185, 198
AcoI YGGCCR 1 cut(s) 307
AcvI CACGTG 1 cut(s) 87
AfaI GTAC 1 cut(s) 49
AfeI AGCGCT 1 cut(s) 221
AflIII ACRYGT 2 cut(s) 84, 86
AjuI GAANNNNNNNTTGG 2 cut(s) 312, 344
AluBI AGCT 5 cut(s) 262, 359, 368, 608, 699
AluI AGCT 5 cut(s) 262, 359, 368, 608, 699
Alw21I GWGCWC 1 cut(s) 581
Alw26I GTCTC 2 cut(s) 25, 491
AlwI GGATC 2 cut(s) 185, 198
Aor51HI AGCGCT 1 cut(s) 221
AoxI GGCC 5 cut(s) 279, 307, 456, 472, 693
ApeKI GCWGC 2 cut(s) 101, 365
AseI ATTAAT 1 cut(s) 179
Asp700I GAANNNNTTC 1 cut(s) 344
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 2 cut(s) 279, 473
AsuHPI GGTGA 2 cut(s) 92, 733
BalI TGGCCA 1 cut(s) 309
BamHI GGATCC 1 cut(s) 190
BbrPI CACGTG 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 581
BbvI GCAGC 2 cut(s) 88, 377
BciVI GTATCC 2 cut(s) 259, 595
BcoDI GTCTC 2 cut(s) 25, 491
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 1 cut(s) 223
BfuI GTATCC 2 cut(s) 259, 595
BisI GCNGC 3 cut(s) 99, 102, 366
BlsI GCNGC 3 cut(s) 100, 103, 367
BmgT120I GGNCC 2 cut(s) 279, 473
BmiI GGNNCC 2 cut(s) 192, 347
BmsI GCATC 1 cut(s) 163
BpuEI CTTGAG 2 cut(s) 515, 661
BsaAI YACGTR 2 cut(s) 87, 529
BsaJI CCNNGG 6 cut(s) 61, 252, 324, 349, 403, 589
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 345, 375
Bse118I RCCGGY 1 cut(s) 39
BseDI CCNNGG 6 cut(s) 61, 252, 324, 349, 403, 589
BseGI GGATG 3 cut(s) 178, 262, 732
BseMII CTCAG 2 cut(s) 231, 266
BseXI GCAGC 2 cut(s) 88, 377
Bsh1236I CGCG 2 cut(s) 117, 326
BshFI GGCC 5 cut(s) 281, 309, 458, 474, 695
BsiHKAI GWGCWC 1 cut(s) 581
BsiSI CCGG 1 cut(s) 40
BsmAI GTCTC 2 cut(s) 25, 491
BsmBI CGTCTC 1 cut(s) 491
BsmI GAATGC 1 cut(s) 674
BsnI GGCC 5 cut(s) 281, 309, 458, 474, 695
Bsp1286I GDGCHC 1 cut(s) 581
Bsp1407I TGTACA 1 cut(s) 47
Bsp143I GATC 1 cut(s) 190
Bsp19I CCATGG 1 cut(s) 349
BspACI CCGC 5 cut(s) 99, 117, 324, 326, 400
BspANI GGCC 5 cut(s) 281, 309, 458, 474, 695
BspCNI CTCAG 2 cut(s) 232, 267
BspFNI CGCG 2 cut(s) 117, 326
BspLI GGNNCC 2 cut(s) 192, 347
BspPI GGATC 2 cut(s) 185, 198
BsrFI RCCGGY 1 cut(s) 39
BsrGI TGTACA 1 cut(s) 47
BssAI RCCGGY 1 cut(s) 39
BssECI CCNNGG 6 cut(s) 61, 252, 324, 349, 403, 589
BssMI GATC 1 cut(s) 190
BssT1I CCWWGG 5 cut(s) 61, 252, 349, 403, 589
Bst4CI ACNGT 3 cut(s) 148, 538, 583
BstAUI TGTACA 1 cut(s) 47
BstBAI YACGTR 2 cut(s) 87, 529
BstC8I GCNNGC 2 cut(s) 370, 697
BstDEI CTNAG 2 cut(s) 240, 275
BstDSI CCRYGG 2 cut(s) 324, 349
BstF5I GGATG 3 cut(s) 178, 262, 732
BstFNI CGCG 2 cut(s) 117, 326
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 2 cut(s) 25, 491
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 2 cut(s) 365, 696
BstSFI CTRYAG 1 cut(s) 735
BstUI CGCG 2 cut(s) 117, 326
BstV1I GCAGC 2 cut(s) 88, 377
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BsuI GTATCC 2 cut(s) 259, 595
BsuRI GGCC 5 cut(s) 281, 309, 458, 474, 695
BtgI CCRYGG 2 cut(s) 324, 349
BtgZI GCGATG 1 cut(s) 58
BtsCI GGATG 3 cut(s) 178, 262, 732
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 2 cut(s) 370, 697
CfoI GCGC 1 cut(s) 222
Cfr10I RCCGGY 1 cut(s) 39
Cfr13I GGNCC 2 cut(s) 279, 473
Cfr42I CCGCGG 1 cut(s) 327
Csp6I GTAC 1 cut(s) 48
CviAII CATG 5 cut(s) 225, 311, 350, 373, 487
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 2 cut(s) 240, 275
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 307
Eco130I CCWWGG 5 cut(s) 61, 252, 349, 403, 589
Eco47III AGCGCT 1 cut(s) 221
Eco72I CACGTG 1 cut(s) 87
EcoT14I CCWWGG 5 cut(s) 61, 252, 349, 403, 589
EcoT22I ATGCAT 1 cut(s) 178
ErhI CCWWGG 5 cut(s) 61, 252, 349, 403, 589
Esp3I CGTCTC 1 cut(s) 491
FaeI CATG 5 cut(s) 228, 314, 353, 376, 490
FatI CATG 5 cut(s) 224, 310, 349, 372, 486
Fnu4HI GCNGC 3 cut(s) 99, 102, 366
FokI GGATG 3 cut(s) 185, 269, 739
Fsp4HI GCNGC 3 cut(s) 99, 102, 366
GlaI GCGC 1 cut(s) 221
GluI GCNGC 3 cut(s) 99, 102, 366
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 5 cut(s) 281, 309, 458, 474, 695
HapII CCGG 1 cut(s) 40
HhaI GCGC 1 cut(s) 222
Hin1II CATG 5 cut(s) 228, 314, 353, 376, 490
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HincII GTYRAC 2 cut(s) 439, 586
HindII GTYRAC 2 cut(s) 439, 586
HinfI GANTC 5 cut(s) 26, 136, 419, 482, 490
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 1 cut(s) 40
HphI GGTGA 2 cut(s) 92, 733
Hpy166II GTNNAC 3 cut(s) 272, 439, 586
Hpy188I TCNGA 2 cut(s) 135, 141
Hpy188III TCNNGA 3 cut(s) 30, 479, 494
Hpy8I GTNNAC 3 cut(s) 272, 439, 586
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 5 cut(s) 122, 229, 260, 523, 712
HpyCH4III ACNGT 3 cut(s) 148, 538, 583
HpyCH4IV ACGT 5 cut(s) 86, 302, 500, 528, 561
HpyCH4V TGCA 4 cut(s) 176, 314, 365, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 696
HpyF3I CTNAG 2 cut(s) 240, 275
HpySE526I ACGT 5 cut(s) 86, 302, 500, 528, 561
Hsp92II CATG 5 cut(s) 228, 314, 353, 376, 490
HspAI GCGC 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 586
KspI CCGCGG 1 cut(s) 327
Kzo9I GATC 1 cut(s) 190
LmnI GCTCC 1 cut(s) 576
Lsp1109I GCAGC 2 cut(s) 88, 377
LweI GCATC 1 cut(s) 163
MaeII ACGT 5 cut(s) 86, 302, 500, 528, 561
MaeIII GTNAC 4 cut(s) 55, 80, 89, 420
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 581
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 3 cut(s) 124, 289, 547
MluNI TGGCCA 1 cut(s) 309
MlyI GAGTC 3 cut(s) 35, 428, 484
MmeI TCCRAC 2 cut(s) 164, 389
MnlI CCTC 6 cut(s) 59, 156, 270, 486, 610, 717
Mox20I TGGCCA 1 cut(s) 309
Mph1103I ATGCAT 1 cut(s) 178
MroXI GAANNNNTTC 1 cut(s) 344
MscI TGGCCA 1 cut(s) 309
MseI TTAA 6 cut(s) 123, 179, 288, 387, 585, 632
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 309
MspA1I CMGCKG 4 cut(s) 101, 326, 368, 608
MspI CCGG 1 cut(s) 40
Mva1269I GAATGC 1 cut(s) 674
MvnI CGCG 2 cut(s) 117, 326
MwoI GCNNNNNNNGC 2 cut(s) 365, 696
NcoI CCATGG 1 cut(s) 349
NdeII GATC 1 cut(s) 190
NlaIII CATG 5 cut(s) 228, 314, 353, 376, 490
NlaIV GGNNCC 2 cut(s) 192, 347
NmuCI GTSAC 3 cut(s) 80, 89, 420
NsiI ATGCAT 1 cut(s) 178
PctI GAATGC 1 cut(s) 674
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 2 cut(s) 136, 482
PkrI GCNGC 3 cut(s) 100, 103, 367
PleI GAGTC 3 cut(s) 34, 427, 484
PmaCI CACGTG 1 cut(s) 87
PmlI CACGTG 1 cut(s) 87
PpsI GAGTC 3 cut(s) 34, 427, 484
Ppu21I YACGTR 2 cut(s) 87, 529
PshBI ATTAAT 1 cut(s) 179
PsiI TTATAA 1 cut(s) 624
PspCI CACGTG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 192, 347
PspPI GGNCC 2 cut(s) 279, 473
PsuI RGATCY 1 cut(s) 190
PvuII CAGCTG 2 cut(s) 368, 608
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 1 cut(s) 377
SacII CCGCGG 1 cut(s) 327
SaqAI TTAA 6 cut(s) 123, 179, 288, 387, 585, 632
SatI GCNGC 3 cut(s) 99, 102, 366
Sau3AI GATC 1 cut(s) 190
Sau96I GGNCC 2 cut(s) 279, 473
SchI GAGTC 3 cut(s) 35, 428, 484
SduI GDGCHC 1 cut(s) 581
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 1 cut(s) 735
Sfr303I CCGCGG 1 cut(s) 327
SgrBI CCGCGG 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 494, 640
SmoI CTYRAG 2 cut(s) 494, 640
Sse9I AATT 3 cut(s) 124, 289, 547
SsiI CCGC 5 cut(s) 99, 117, 324, 326, 400
StyI CCWWGG 5 cut(s) 61, 252, 349, 403, 589
TaaI ACNGT 3 cut(s) 148, 538, 583
TaiI ACGT 5 cut(s) 89, 305, 503, 531, 564
TaqI TCGA 2 cut(s) 29, 543
TasI AATT 3 cut(s) 124, 289, 547
TatI WGTACW 1 cut(s) 47
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 2 cut(s) 136, 482
Tru1I TTAA 6 cut(s) 123, 179, 288, 387, 585, 632
Tru9I TTAA 6 cut(s) 123, 179, 288, 387, 585, 632
TscAI CASTG 1 cut(s) 586
TseFI GTSAC 3 cut(s) 80, 89, 420
TseI GCWGC 2 cut(s) 101, 365
Tsp45I GTSAC 3 cut(s) 80, 89, 420
TspDTI ATGAA 4 cut(s) 17, 118, 248, 717
TspRI CASTG 1 cut(s) 586
VspI ATTAAT 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 344
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.