Rh2CG129300

3-dehydroquinate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0006294
Physical Location & Seq
Reverse (-)
11525095 .. 11528512
3418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG129300.1

Sequence Viewer

Length: 1146 bp
ATGGCCTCCACCGCCAACCCCTTCTCCATCTCCCTCTCCTCCAAGCCCACCGCCAAACCCTCACTCTTCAACCCCTCGGAGCTCTCTCTTCGGGTCCAGACCCCCGGCGTCGCGGCTCTCCGGTCTTCCTTCGCTTCCAATTGCTCCGTTAAGCTTAGCCTAGCGAAATCGAAGTCGAAGATAATGGCGAATTCGGCGCAGCTGGTGGATCAACCGCCGAGTAAAACGGCTCCGGTCGTTGTGGATGTGGATTTGGGCAATCGGAGCTACCCGATTTACATTGGATCCGGACTTCTTGACCAACCCGAGCTTCTCCAAAGGCATGTTCATGGAAAGAGAGTGCTGGTGGTGACTAACACCACGGTTGCGCCATTGTATTTGGACAAAGTTGTTGATGCTTTGACGAAAGATAACCCCAATGTTACAGTTGAGAGTGTGATTTTACCGGATGGGGAGAAGTACAAGGACATGGATACTCTTATGAAAGTGTTTGACAAGGCCATTGAGTCAAGACTGGACCGGCGATGTACATTTGTTGCCCTTGGAGGTGGTGTGGTTGGTGACATGTGTGGCTACGCTGCTGCTTCTTTCCTTCGCGGTGTTAATTTCATTCAGATTCCGACAACTGTTATGGCACAGGTTGATTCTTCTGTTGGGGGAAAAACTGGGATCAACCACCCTCTAGGGAAGAACTTGATTGGTGCTTTTTACCAGCCCCAGTGTGTGCTTATCGACACGGACACACTAAATACGTTACCAGATAGGGAACTGGCATCAGGGCTTGCTGAAGTCATTAAGTACGGACTTATTAGAGATGCAGAATTCTTTGAGTGGCAGGAGAGGAATATGGACGCATTAATAGCAAGGGATGCAAGTGCTCTCGCTTATGCTATAAAGCGCTCATGTGAAAATAAGGCTGAGGTTGTGTCCTTGGATGAGAAAGAAGGTGGACTGAGGGCAACTCTGAATTTGGGCCATACATTTGGCCATGCAATAGAAACAGGGGTTGGCTATGGGAAGTGGTTCCATGGAGAAGCTGTTGCAGCTGGCATGGTAATAATCCTCTACTTTTGCACCTTTTGTTCCTTAAAAAATCTTTTTTTCAATGATGAACGTTATCTCATATGTTTAGGTCATGGCTGTTGA

Protein Analysis

381

Amino Acids

41.23

Weight (kDa)

6.66

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-ADH PF00465 92 - 210 2.8e-06 Iron-containing alcohol dehydrogenase
Fe-ADH_2 PF13685 96 - 251 2.2e-09 Iron-containing alcohol dehydrogenase
DHQ_synthase PF01761 146 - 258 1.4e-48 3-dehydroquinate synthase N-terminal
DHQS_C PF24621 260 - 356 8e-40 3-dehydroquinate synthase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 113, 597
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 5 cut(s) 12, 51, 113, 215, 597
AclI AACGTT 1 cut(s) 1114
AclWI GGATC 4 cut(s) 216, 279, 292, 677
AcoI YGGCCR 1 cut(s) 985
AcsI RAATTY 3 cut(s) 190, 821, 967
AcuI CTGAAG 1 cut(s) 807
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 3 cut(s) 461, 529, 800
AfeI AGCGCT 1 cut(s) 899
AflIII ACRYGT 1 cut(s) 564
AgsI TTSAA 2 cut(s) 70, 1105
AjuI GAANNNNNNNTTGG 6 cut(s) 294, 309, 326, 341, 990, 1022
AluBI AGCT 7 cut(s) 82, 154, 202, 267, 310, 1037, 1046
AluI AGCT 7 cut(s) 82, 154, 202, 267, 310, 1037, 1046
Alw21I GWGCWC 2 cut(s) 84, 880
AlwI GGATC 4 cut(s) 216, 279, 292, 677
Ama87I CYCGRG 1 cut(s) 305
Aor13HI TCCGGA 1 cut(s) 287
Aor51HI AGCGCT 1 cut(s) 899
AoxI GGCC 4 cut(s) 3, 498, 973, 985
ApeKI GCWGC 4 cut(s) 199, 578, 581, 1043
ApoI RAATTY 3 cut(s) 190, 821, 967
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AseI ATTAAT 1 cut(s) 857
Asp700I GAANNNNTTC 1 cut(s) 1022
AspLEI GCGC 3 cut(s) 199, 370, 900
AspS9I GGNCC 3 cut(s) 94, 517, 973
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 2 cut(s) 361, 572
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 2 cut(s) 94, 517
BalI TGGCCA 1 cut(s) 987
BamHI GGATCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 84, 880
BbvCI CCTCAGC 1 cut(s) 918
BbvI GCAGC 4 cut(s) 211, 565, 568, 1055
BccI CCATC 2 cut(s) 35, 443
BceAI ACGGC 1 cut(s) 243
BciVI GTATCC 1 cut(s) 466
BcnI CCSGG 1 cut(s) 105
BfaI CTAG 2 cut(s) 161, 683
BfoI RGCGCY 1 cut(s) 901
BfuI GTATCC 1 cut(s) 466
BisI GCNGC 5 cut(s) 114, 200, 579, 582, 1044
BlpI GCTNAGC 1 cut(s) 155
BlsI GCNGC 5 cut(s) 115, 201, 580, 583, 1045
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 2 cut(s) 94, 517
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 3 cut(s) 94, 517, 973
BmiI GGNNCC 4 cut(s) 95, 231, 286, 1025
BmrFI CCNGG 1 cut(s) 105
BmrI ACTGGG 2 cut(s) 675, 712
BmsI GCATC 4 cut(s) 385, 782, 805, 859
BmuI ACTGGG 2 cut(s) 675, 712
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 946, 978
Bpu10I CCTNAGC 1 cut(s) 918
Bpu1102I GCTNAGC 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 6 cut(s) 75, 103, 360, 541, 930, 1027
BsaWI WCCGGW 4 cut(s) 120, 232, 287, 445
BsaXI ACNNNNNCTCC 6 cut(s) 8, 38, 537, 567, 1023, 1053
Bse118I RCCGGY 1 cut(s) 519
Bse1I ACTGG 4 cut(s) 519, 670, 718, 774
BseAI TCCGGA 1 cut(s) 287
BseDI CCNNGG 6 cut(s) 75, 103, 360, 541, 930, 1027
BseGI GGATG 4 cut(s) 250, 454, 874, 940
BseMII CTCAG 2 cut(s) 909, 944
BseNI ACTGG 4 cut(s) 519, 670, 718, 774
BseRI GAGGAG 1 cut(s) 28
BseXI GCAGC 4 cut(s) 211, 565, 568, 1055
Bsh1236I CGCG 2 cut(s) 113, 597
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 4 cut(s) 5, 500, 975, 987
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 84, 880
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 6 cut(s) 105, 121, 233, 288, 446, 520
BsnI GGCC 4 cut(s) 5, 500, 975, 987
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 84, 880
Bsp13I TCCGGA 1 cut(s) 287
Bsp1407I TGTACA 1 cut(s) 527
Bsp143I GATC 3 cut(s) 208, 284, 669
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp19I CCATGG 1 cut(s) 1027
BspACI CCGC 5 cut(s) 12, 51, 113, 215, 597
BspANI GGCC 4 cut(s) 5, 500, 975, 987
BspCNI CTCAG 2 cut(s) 910, 945
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 2 cut(s) 113, 597
BspLI GGNNCC 4 cut(s) 95, 231, 286, 1025
BspPI GGATC 4 cut(s) 216, 279, 292, 677
BsrFI RCCGGY 1 cut(s) 519
BsrGI TGTACA 1 cut(s) 527
BsrI ACTGG 4 cut(s) 519, 670, 718, 774
BssAI RCCGGY 1 cut(s) 519
BssECI CCNNGG 6 cut(s) 75, 103, 360, 541, 930, 1027
BssMI GATC 3 cut(s) 208, 284, 669
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 3 cut(s) 541, 930, 1027
Bst4CI ACNGT 3 cut(s) 364, 427, 628
Bst6I CTCTTC 2 cut(s) 71, 93
BstACI GRCGYC 1 cut(s) 108
BstAPI GCANNNNNTGC 1 cut(s) 869
BstAUI TGTACA 1 cut(s) 527
BstC8I GCNNGC 2 cut(s) 783, 1048
BstDEI CTNAG 3 cut(s) 155, 918, 953
BstDSI CCRYGG 2 cut(s) 360, 1027
BstF5I GGATG 4 cut(s) 250, 454, 874, 940
BstFNI CGCG 2 cut(s) 113, 597
BstH2I RGCGCY 1 cut(s) 901
BstHHI GCGC 3 cut(s) 199, 370, 900
BstKTI GATC 3 cut(s) 211, 287, 672
BstMBI GATC 3 cut(s) 208, 284, 669
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 6 cut(s) 11, 194, 264, 860, 869, 1043
BstNSI RCATGY 2 cut(s) 326, 568
BstSCI CCNGG 1 cut(s) 103
BstUI CGCG 2 cut(s) 113, 597
BstV1I GCAGC 4 cut(s) 211, 565, 568, 1055
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 284
BstXI CCANNNNNNTGG 1 cut(s) 983
BstYI RGATCY 1 cut(s) 284
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 4 cut(s) 5, 500, 975, 987
BtgI CCRYGG 2 cut(s) 360, 1027
BtgZI GCGATG 1 cut(s) 538
BtsCI GGATG 4 cut(s) 250, 454, 874, 940
BtsIMutI CAGTG 1 cut(s) 725
Cac8I GCNNGC 2 cut(s) 783, 1048
CfoI GCGC 3 cut(s) 199, 370, 900
Cfr10I RCCGGY 1 cut(s) 519
Cfr13I GGNCC 3 cut(s) 94, 517, 973
CseI GACGC 2 cut(s) 97, 860
Csp6I GTAC 3 cut(s) 460, 528, 799
CviAII CATG 9 cut(s) 323, 329, 469, 565, 903, 989, 1028, 1051, 1136
CviQI GTAC 3 cut(s) 460, 528, 799
DdeI CTNAG 3 cut(s) 155, 918, 953
DpnI GATC 3 cut(s) 210, 286, 671
DpnII GATC 3 cut(s) 208, 284, 669
EaeI YGGCCR 1 cut(s) 985
Eam1104I CTCTTC 2 cut(s) 71, 93
EarI CTCTTC 2 cut(s) 71, 93
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 3 cut(s) 541, 930, 1027
Eco24I GRGCYC 1 cut(s) 84
Eco47I GGWCC 2 cut(s) 94, 517
Eco47III AGCGCT 1 cut(s) 899
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 807
Eco88I CYCGRG 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 82
EcoRI GAATTC 2 cut(s) 190, 821
EcoT14I CCWWGG 3 cut(s) 541, 930, 1027
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 3 cut(s) 541, 930, 1027
FaeI CATG 9 cut(s) 326, 332, 472, 568, 906, 992, 1031, 1054, 1139
FatI CATG 9 cut(s) 322, 328, 468, 564, 902, 988, 1027, 1050, 1135
FauNDI CATATG 1 cut(s) 1124
Fnu4HI GCNGC 5 cut(s) 114, 200, 579, 582, 1044
FokI GGATG 4 cut(s) 257, 461, 881, 947
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 5 cut(s) 114, 200, 579, 582, 1044
FspBI CTAG 2 cut(s) 161, 683
GlaI GCGC 3 cut(s) 198, 369, 899
GluI GCNGC 5 cut(s) 114, 200, 579, 582, 1044
HaeII RGCGCY 1 cut(s) 901
HaeIII GGCC 4 cut(s) 5, 500, 975, 987
HapII CCGG 6 cut(s) 105, 121, 233, 288, 446, 520
HgaI GACGC 2 cut(s) 97, 860
HhaI GCGC 3 cut(s) 199, 370, 900
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 9 cut(s) 326, 332, 472, 568, 906, 992, 1031, 1054, 1139
Hin6I GCGC 3 cut(s) 197, 368, 898
HinP1I GCGC 3 cut(s) 197, 368, 898
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 3 cut(s) 506, 616, 644
HpaII CCGG 6 cut(s) 105, 121, 233, 288, 446, 520
HphI GGTGA 2 cut(s) 361, 572
Hpy166II GTNNAC 1 cut(s) 950
Hpy188I TCNGA 5 cut(s) 79, 264, 615, 621, 966
Hpy188III TCNNGA 4 cut(s) 97, 288, 296, 510
Hpy8I GTNNAC 1 cut(s) 950
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 4 cut(s) 31, 139, 602, 938
HpyCH4III ACNGT 3 cut(s) 364, 427, 628
HpyCH4IV ACGT 2 cut(s) 752, 1114
HpyCH4V TGCA 5 cut(s) 818, 872, 992, 1043, 1074
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 194, 264, 860, 869, 1043
HpyF3I CTNAG 3 cut(s) 155, 918, 953
HpySE526I ACGT 2 cut(s) 752, 1114
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 9 cut(s) 326, 332, 472, 568, 906, 992, 1031, 1054, 1139
HspAI GCGC 3 cut(s) 197, 368, 898
Kpn2I TCCGGA 1 cut(s) 287
Kzo9I GATC 3 cut(s) 208, 284, 669
LmnI GCTCC 4 cut(s) 79, 149, 235, 264
Lsp1109I GCAGC 4 cut(s) 211, 565, 568, 1055
LweI GCATC 4 cut(s) 385, 782, 805, 859
MaeI CTAG 2 cut(s) 161, 683
MaeII ACGT 2 cut(s) 752, 1114
MaeIII GTNAC 4 cut(s) 349, 421, 560, 753
MalI GATC 3 cut(s) 210, 286, 671
MboI GATC 3 cut(s) 208, 284, 669
MboII GAAGA 6 cut(s) 58, 80, 117, 190, 639, 700
MfeI CAATTG 1 cut(s) 139
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 2 cut(s) 84, 880
MlsI TGGCCA 1 cut(s) 987
MluCI AATT 5 cut(s) 139, 190, 604, 821, 967
MluNI TGGCCA 1 cut(s) 987
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 1 cut(s) 644
Mox20I TGGCCA 1 cut(s) 987
MroI TCCGGA 1 cut(s) 287
MroXI GAANNNNTTC 1 cut(s) 1022
MscI TGGCCA 1 cut(s) 987
MseI TTAA 5 cut(s) 150, 603, 795, 857, 1088
MslI CAYNNNNRTG 1 cut(s) 327
Msp20I TGGCCA 1 cut(s) 987
MspA1I CMGCKG 2 cut(s) 202, 1046
MspI CCGG 6 cut(s) 105, 121, 233, 288, 446, 520
MspR9I CCNGG 1 cut(s) 105
MunI CAATTG 1 cut(s) 139
MvnI CGCG 2 cut(s) 113, 597
MwoI GCNNNNNNNGC 6 cut(s) 11, 194, 264, 860, 869, 1043
NciI CCSGG 1 cut(s) 105
NcoI CCATGG 1 cut(s) 1027
NdeI CATATG 1 cut(s) 1124
NdeII GATC 3 cut(s) 208, 284, 669
NlaIII CATG 9 cut(s) 326, 332, 472, 568, 906, 992, 1031, 1054, 1139
NlaIV GGNNCC 4 cut(s) 95, 231, 286, 1025
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 2 cut(s) 349, 560
NspI RCATGY 2 cut(s) 326, 568
PciI ACATGT 1 cut(s) 564
PdmI GAANNNNTTC 1 cut(s) 1022
PfeI GAWTC 2 cut(s) 616, 644
PkrI GCNGC 5 cut(s) 115, 201, 580, 583, 1045
PleI GAGTC 1 cut(s) 514
PpsI GAGTC 1 cut(s) 514
PscI ACATGT 1 cut(s) 564
PshBI ATTAAT 1 cut(s) 857
Psp124BI GAGCTC 1 cut(s) 84
Psp1406I AACGTT 1 cut(s) 1114
PspN4I GGNNCC 4 cut(s) 95, 231, 286, 1025
PspPI GGNCC 3 cut(s) 94, 517, 973
PsuI RGATCY 1 cut(s) 284
PvuII CAGCTG 2 cut(s) 202, 1046
RsaI GTAC 3 cut(s) 461, 529, 800
RsaNI GTAC 3 cut(s) 460, 528, 799
RseI CAYNNNNRTG 1 cut(s) 327
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 5 cut(s) 150, 603, 795, 857, 1088
SatI GCNGC 5 cut(s) 114, 200, 579, 582, 1044
Sau3AI GATC 3 cut(s) 208, 284, 669
Sau96I GGNCC 3 cut(s) 94, 517, 973
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 2 cut(s) 84, 880
SfaNI GCATC 4 cut(s) 385, 782, 805, 859
SinI GGWCC 2 cut(s) 94, 517
SmiMI CAYNNNNRTG 1 cut(s) 327
Sse9I AATT 5 cut(s) 139, 190, 604, 821, 967
SsiI CCGC 5 cut(s) 12, 51, 113, 215, 597
SspMI CTAG 2 cut(s) 161, 683
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 3 cut(s) 541, 930, 1027
TaaI ACNGT 3 cut(s) 364, 427, 628
TaiI ACGT 2 cut(s) 755, 1117
TaqI TCGA 3 cut(s) 170, 176, 732
TasI AATT 5 cut(s) 139, 190, 604, 821, 967
TatI WGTACW 2 cut(s) 459, 527
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 2 cut(s) 616, 644
Tru1I TTAA 5 cut(s) 150, 603, 795, 857, 1088
Tru9I TTAA 5 cut(s) 150, 603, 795, 857, 1088
TscAI CASTG 1 cut(s) 725
TseFI GTSAC 2 cut(s) 349, 560
TseI GCWGC 4 cut(s) 199, 578, 581, 1043
Tsp45I GTSAC 2 cut(s) 349, 560
TspDTI ATGAA 4 cut(s) 317, 497, 598, 1125
TspGWI ACGGA 3 cut(s) 136, 752, 816
TspRI CASTG 1 cut(s) 725
VpaK11BI GGWCC 2 cut(s) 94, 517
VspI ATTAAT 1 cut(s) 857
XapI RAATTY 3 cut(s) 190, 821, 967
XceI RCATGY 2 cut(s) 326, 568
XmnI GAANNNNTTC 1 cut(s) 1022
XspI CTAG 2 cut(s) 161, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.