FvH4_1g01850

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004831
Physical Location & Seq
Forward (+)
966826 .. 969016
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01850.t1

Sequence Viewer

Length: 1143 bp
ATGCGTGGAACTTGTTCCAATGTCGATGATTCCCTTCAAGAACAACCCTCTGAGCAGCCAATATTAATATGCGAGTGTTCCAATGAGGGAAATTGCGGATGTAGTAATAAAAGGATGATTTCTGCTGGAGATGTTGGATTACTTGCTACTTTCGAATCCGAGAGGGGCAATGGCCACCTTTTATCTCTTCTTGCATCTTCATCTTCATCCTCATCCCAAATATCACATTCACATCAACCTTCAAAACCTACAAGCAGCCAGGTTTCAAACAAGTCAGAATGTACATCATCAATGGTCGACAATCATAACTCGTCGCGAAGACTAATAGTCCGAAGGAACTCGAACCCTAATCCAGAAGCTACAATACGAGGCCTAACAAGTTGCATGAACACACCGAACCTCGTTAACTCTGGAAATTGCACCACACTTCTTTTCGGATCCACTCCTCTTTGCCAATTCTCTCTTTCTCAAGGTCTTTTTGGGGCGCCTTCGAATCTTTTCCAGGCTGTAATGCCTGCCATTCAGCTTCCCCACGCAAGTGGAGATTCAACATCTCAAATAGAAGAAGCTCCTGCTACTTTTGATGATAGTGGATTCTCTAGTGAGAACCAACTAATTGATATATGGAGGCAACTCCTCATGGAATCGACTCTATATATACACAGGCCGGTTGATAACTCGTTAGAAGGTTCAAGTAGTTCCGTTTTTAGGGTAACTGCTGACACTGGATCTGTGGACTTCAGACTTTTAACAGAGAACGCTGAAGTTGTTAACCAATCACTGCTGTACTTTTCAATCGAAATGCGCAATCGCTTTGGAGGCATGAGGCTTGATGTGGATAACATGTCATATGAGGAGCTATTGGCTTTAGAAGATCGTATAGGGTATGTCAGCACAGGATTATCGGAGGAAGCAGTCGTTGCATCTCTCAAACTCAGTAAATATTTTCTGCTTGCTGATGAAAATGCTGAGAAAGAGTTTTGCAGCATCTGCCAGGAGGATTTTATAGAGGAAGATGAGCTAGGAACATTGGATTGCGGACACGGCTTCCACATTTGTTGCATCAAACAATGGCTAGGATATAAAAACGTGTGCCCTATATGCAAATCTCCTGCCTTATCTACTACATTTTCACATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

381

Amino Acids

41.47

Weight (kDa)

4.75

Isoelectric Point (pI)

55.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 325 - 369 1.2e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 328 - 369 3.4e-12 Ring finger domain
zf-C3HC4 PF00097 328 - 368 1.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 328 - 368 1.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 806
AccB1I GGYRCC 1 cut(s) 484
AccI GTMKAC 1 cut(s) 297
AccII CGCG 1 cut(s) 316
AciI CCGC 2 cut(s) 96, 1038
AclWI GGATC 3 cut(s) 432, 445, 736
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 2 cut(s) 724, 783
AcyI GRCGYC 1 cut(s) 485
AfaI GTAC 2 cut(s) 283, 788
AfiI CCNNNNNNNGG 1 cut(s) 708
AflIII ACRYGT 2 cut(s) 843, 1089
AgsI TTSAA 6 cut(s) 38, 243, 267, 549, 693, 795
AhdI GACNNNNNGTC 1 cut(s) 326
AjnI CCWGG 3 cut(s) 258, 501, 993
AleI CACNNNNGTG 1 cut(s) 537
AluBI AGCT 5 cut(s) 359, 526, 569, 859, 1021
AluI AGCT 5 cut(s) 359, 526, 569, 859, 1021
AlwI GGATC 3 cut(s) 432, 445, 736
AlwNI CAGNNNCTG 1 cut(s) 990
AoxI GGCC 3 cut(s) 172, 370, 665
ApeKI GCWGC 3 cut(s) 55, 255, 984
AseI ATTAAT 1 cut(s) 65
Asp700I GAANNNNTTC 2 cut(s) 13, 497
AspLEI GCGC 2 cut(s) 487, 807
AsuII TTCGAA 2 cut(s) 153, 491
BaeGI GKGCMC 1 cut(s) 1097
BalI TGGCCA 1 cut(s) 174
BamHI GGATCC 1 cut(s) 437
BanI GGYRCC 1 cut(s) 484
BarI GAAGNNNNNNTAC 2 cut(s) 348, 380
BbsI GAAGAC 1 cut(s) 325
BbvI GCAGC 3 cut(s) 67, 267, 996
BceAI ACGGC 1 cut(s) 1060
BciT130I CCWGG 3 cut(s) 260, 503, 995
BfaI CTAG 3 cut(s) 600, 1022, 1076
BfoI RGCGCY 1 cut(s) 488
BisI GCNGC 3 cut(s) 56, 256, 985
BlsI GCNGC 3 cut(s) 57, 257, 986
Bme1390I CCNGG 3 cut(s) 260, 503, 995
BmeRI GACNNNNNGTC 1 cut(s) 326
BmiI GGNNCC 2 cut(s) 439, 486
BmrFI CCNGG 3 cut(s) 260, 503, 995
BmsI GCATC 4 cut(s) 203, 932, 996, 1071
BpiI GAAGAC 1 cut(s) 325
BpmI CTGGAG 1 cut(s) 147
Bpu14I TTCGAA 2 cut(s) 153, 491
BpuEI CTTGAG 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 485
Bsc4I CCNNNNNNNGG 1 cut(s) 708
Bse118I RCCGGY 1 cut(s) 667
Bse1I ACTGG 1 cut(s) 730
Bse3DI GCAATG 1 cut(s) 175
BseBI CCWGG 3 cut(s) 260, 503, 995
BseGI GGATG 4 cut(s) 104, 120, 206, 212
BseLI CCNNNNNNNGG 1 cut(s) 708
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 3 cut(s) 42, 949, 960
BseNI ACTGG 1 cut(s) 730
BseRI GAGGAG 3 cut(s) 435, 626, 869
BseSI GKGCMC 1 cut(s) 1097
BseXI GCAGC 3 cut(s) 67, 267, 996
Bsh1236I CGCG 1 cut(s) 316
BshFI GGCC 3 cut(s) 174, 372, 667
BshNI GGYRCC 1 cut(s) 484
BsiSI CCGG 1 cut(s) 668
BslI CCNNNNNNNGG 1 cut(s) 708
BsnI GGCC 3 cut(s) 174, 372, 667
Bsp119I TTCGAA 2 cut(s) 153, 491
Bsp1286I GDGCHC 1 cut(s) 1097
Bsp1407I TGTACA 1 cut(s) 281
Bsp143I GATC 3 cut(s) 437, 728, 874
Bsp68I TCGCGA 1 cut(s) 316
BspACI CCGC 2 cut(s) 96, 1038
BspANI GGCC 3 cut(s) 174, 372, 667
BspCNI CTCAG 3 cut(s) 43, 948, 961
BspFNI CGCG 1 cut(s) 316
BspLI GGNNCC 2 cut(s) 439, 486
BspPI GGATC 3 cut(s) 432, 445, 736
BspT104I TTCGAA 2 cut(s) 153, 491
BspT107I GGYRCC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 667
BsrGI TGTACA 1 cut(s) 281
BsrI ACTGG 1 cut(s) 730
BssAI RCCGGY 1 cut(s) 667
BssMI GATC 3 cut(s) 437, 728, 874
BssNI GRCGYC 1 cut(s) 485
Bst2UI CCWGG 3 cut(s) 260, 503, 995
Bst6I CTCTTC 1 cut(s) 192
BstACI GRCGYC 1 cut(s) 485
BstAPI GCANNNNNTGC 2 cut(s) 920, 990
BstAUI TGTACA 1 cut(s) 281
BstBI TTCGAA 2 cut(s) 153, 491
BstC8I GCNNGC 2 cut(s) 516, 954
BstDEI CTNAG 3 cut(s) 51, 935, 969
BstF5I GGATG 4 cut(s) 104, 120, 206, 212
BstFNI CGCG 1 cut(s) 316
BstH2I RGCGCY 1 cut(s) 488
BstHHI GCGC 2 cut(s) 487, 807
BstKTI GATC 3 cut(s) 440, 731, 877
BstMBI GATC 3 cut(s) 437, 728, 874
BstMWI GCNNNNNNNGC 5 cut(s) 819, 920, 990, 1044, 1101
BstNI CCWGG 3 cut(s) 260, 503, 995
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 3 cut(s) 258, 501, 993
BstSLI GKGCMC 1 cut(s) 1097
BstUI CGCG 1 cut(s) 316
BstV1I GCAGC 3 cut(s) 67, 267, 996
BstV2I GAAGAC 1 cut(s) 325
BstX2I RGATCY 2 cut(s) 437, 728
BstXI CCANNNNNNTGG 1 cut(s) 539
BstYI RGATCY 2 cut(s) 437, 728
BsuRI GGCC 3 cut(s) 174, 372, 667
BtsCI GGATG 4 cut(s) 104, 120, 206, 212
BtsI GCAGTG 1 cut(s) 779
BtsIMutI CAGTG 2 cut(s) 723, 779
BtuMI TCGCGA 1 cut(s) 316
Cac8I GCNNGC 2 cut(s) 516, 954
CaiI CAGNNNCTG 1 cut(s) 990
CfoI GCGC 2 cut(s) 487, 807
Cfr10I RCCGGY 1 cut(s) 667
Csp6I GTAC 2 cut(s) 282, 787
CspCI CAANNNNNGTGG 2 cut(s) 1040, 1075
CviAII CATG 4 cut(s) 385, 640, 823, 844
CviQI GTAC 2 cut(s) 282, 787
DdeI CTNAG 3 cut(s) 51, 935, 969
DinI GGCGCC 1 cut(s) 486
DpnI GATC 3 cut(s) 439, 730, 876
DpnII GATC 3 cut(s) 437, 728, 874
DriI GACNNNNNGTC 1 cut(s) 326
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 192
Eam1105I GACNNNNNGTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 192
Eco147I AGGCCT 1 cut(s) 372
Eco57I CTGAAG 2 cut(s) 724, 783
EcoRII CCWGG 3 cut(s) 258, 501, 993
EgeI GGCGCC 1 cut(s) 486
EheI GGCGCC 1 cut(s) 486
FaeI CATG 4 cut(s) 388, 643, 826, 847
FatI CATG 4 cut(s) 384, 639, 822, 843
FauNDI CATATG 1 cut(s) 850
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 3 cut(s) 56, 256, 985
FokI GGATG 4 cut(s) 111, 127, 193, 199
Fsp4HI GCNGC 3 cut(s) 56, 256, 985
FspBI CTAG 3 cut(s) 600, 1022, 1076
FspI TGCGCA 1 cut(s) 806
GlaI GCGC 2 cut(s) 486, 806
GluI GCNGC 3 cut(s) 56, 256, 985
GsuI CTGGAG 1 cut(s) 147
HaeII RGCGCY 1 cut(s) 488
HaeIII GGCC 3 cut(s) 174, 372, 667
HapII CCGG 1 cut(s) 668
HhaI GCGC 2 cut(s) 487, 807
Hin1I GRCGYC 1 cut(s) 485
Hin1II CATG 4 cut(s) 388, 643, 826, 847
Hin6I GCGC 2 cut(s) 485, 805
HinP1I GCGC 2 cut(s) 485, 805
HincII GTYRAC 3 cut(s) 298, 406, 772
HindII GTYRAC 3 cut(s) 298, 406, 772
HinfI GANTC 7 cut(s) 29, 155, 493, 545, 594, 644, 649
HpaI GTTAAC 2 cut(s) 406, 772
HpaII CCGG 1 cut(s) 668
Hpy166II GTNNAC 4 cut(s) 298, 406, 736, 772
Hpy188I TCNGA 7 cut(s) 52, 160, 277, 332, 437, 743, 907
Hpy188III TCNNGA 4 cut(s) 38, 315, 353, 411
Hpy8I GTNNAC 4 cut(s) 298, 406, 736, 772
Hpy99I CGWCG 1 cut(s) 316
HpyAV CCTTC 5 cut(s) 44, 249, 327, 498, 680
HpyCH4IV ACGT 1 cut(s) 1089
HpyCH4V TGCA 7 cut(s) 194, 384, 420, 923, 984, 1062, 1104
HpyF10VI GCNNNNNNNGC 5 cut(s) 819, 920, 990, 1044, 1101
HpyF3I CTNAG 3 cut(s) 51, 935, 969
HpySE526I ACGT 1 cut(s) 1089
Hsp92I GRCGYC 1 cut(s) 485
Hsp92II CATG 4 cut(s) 388, 643, 826, 847
HspAI GCGC 2 cut(s) 485, 805
KasI GGCGCC 1 cut(s) 484
KspAI GTTAAC 2 cut(s) 406, 772
Kzo9I GATC 3 cut(s) 437, 728, 874
LmnI GCTCC 2 cut(s) 574, 856
Lsp1109I GCAGC 3 cut(s) 67, 267, 996
LweI GCATC 4 cut(s) 203, 932, 996, 1071
MaeI CTAG 3 cut(s) 600, 1022, 1076
MaeII ACGT 1 cut(s) 1089
MaeIII GTNAC 1 cut(s) 712
MalI GATC 3 cut(s) 439, 730, 876
MboI GATC 3 cut(s) 437, 728, 874
MboII GAAGA 7 cut(s) 179, 189, 195, 330, 575, 884, 1025
MflI RGATCY 2 cut(s) 437, 728
MhlI GDGCHC 1 cut(s) 1097
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 4 cut(s) 91, 415, 455, 615
MluNI TGGCCA 1 cut(s) 174
Mly113I GGCGCC 1 cut(s) 485
MlyI GAGTC 1 cut(s) 643
MmeI TCCRAC 1 cut(s) 115
Mox20I TGGCCA 1 cut(s) 174
MroXI GAANNNNTTC 2 cut(s) 13, 497
MscI TGGCCA 1 cut(s) 174
MseI TTAA 5 cut(s) 65, 405, 749, 771, 1141
MslI CAYNNNNRTG 1 cut(s) 537
Msp20I TGGCCA 1 cut(s) 174
MspI CCGG 1 cut(s) 668
MspR9I CCNGG 3 cut(s) 260, 503, 995
MvaI CCWGG 3 cut(s) 260, 503, 995
MvnI CGCG 1 cut(s) 316
MwoI GCNNNNNNNGC 5 cut(s) 819, 920, 990, 1044, 1101
NarI GGCGCC 1 cut(s) 485
NdeI CATATG 1 cut(s) 850
NdeII GATC 3 cut(s) 437, 728, 874
NlaIII CATG 4 cut(s) 388, 643, 826, 847
NlaIV GGNNCC 2 cut(s) 439, 486
NruI TCGCGA 1 cut(s) 316
NsbI TGCGCA 1 cut(s) 806
NspI RCATGY 1 cut(s) 847
NspV TTCGAA 2 cut(s) 153, 491
OliI CACNNNNGTG 1 cut(s) 537
PceI AGGCCT 1 cut(s) 372
PciI ACATGT 1 cut(s) 843
PdmI GAANNNNTTC 2 cut(s) 13, 497
PfeI GAWTC 6 cut(s) 29, 155, 493, 545, 594, 644
PkrI GCNGC 3 cut(s) 57, 257, 986
PleI GAGTC 1 cut(s) 643
PluTI GGCGCC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 643
PscI ACATGT 1 cut(s) 843
PshBI ATTAAT 1 cut(s) 65
Psp6I CCWGG 3 cut(s) 258, 501, 993
PspGI CCWGG 3 cut(s) 258, 501, 993
PspN4I GGNNCC 2 cut(s) 439, 486
PstNI CAGNNNCTG 1 cut(s) 990
PsuI RGATCY 2 cut(s) 437, 728
RruI TCGCGA 1 cut(s) 316
RsaI GTAC 2 cut(s) 283, 788
RsaNI GTAC 2 cut(s) 282, 787
RseI CAYNNNNRTG 1 cut(s) 537
SalI GTCGAC 1 cut(s) 296
SaqAI TTAA 5 cut(s) 65, 405, 749, 771, 1141
SatI GCNGC 3 cut(s) 56, 256, 985
Sau3AI GATC 3 cut(s) 437, 728, 874
SchI GAGTC 1 cut(s) 643
ScrFI CCNGG 3 cut(s) 260, 503, 995
SduI GDGCHC 1 cut(s) 1097
SfaNI GCATC 4 cut(s) 203, 932, 996, 1071
SfoI GGCGCC 1 cut(s) 486
SfuI TTCGAA 2 cut(s) 153, 491
SmiMI CAYNNNNRTG 1 cut(s) 537
SmlI CTYRAG 1 cut(s) 468
SmoI CTYRAG 1 cut(s) 468
Sse9I AATT 4 cut(s) 91, 415, 455, 615
SseBI AGGCCT 1 cut(s) 372
SsiI CCGC 2 cut(s) 96, 1038
SspDI GGCGCC 1 cut(s) 484
SspI AATATT 2 cut(s) 63, 944
SspMI CTAG 3 cut(s) 600, 1022, 1076
StuI AGGCCT 1 cut(s) 372
StyD4I CCNGG 3 cut(s) 258, 501, 993
TaiI ACGT 1 cut(s) 1092
TaqI TCGA 7 cut(s) 24, 153, 297, 341, 491, 647, 798
TasI AATT 4 cut(s) 91, 415, 455, 615
TatI WGTACW 2 cut(s) 281, 786
TfiI GAWTC 6 cut(s) 29, 155, 493, 545, 594, 644
Tru1I TTAA 5 cut(s) 65, 405, 749, 771, 1141
Tru9I TTAA 5 cut(s) 65, 405, 749, 771, 1141
TscAI CASTG 2 cut(s) 730, 786
TseI GCWGC 3 cut(s) 55, 255, 984
TspDTI ATGAA 4 cut(s) 189, 195, 401, 975
TspGWI ACGGA 1 cut(s) 691
TspRI CASTG 2 cut(s) 730, 786
VspI ATTAAT 1 cut(s) 65
XceI RCATGY 1 cut(s) 847
XmiI GTMKAC 1 cut(s) 297
XmnI GAANNNNTTC 2 cut(s) 13, 497
XspI CTAG 3 cut(s) 600, 1022, 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.