Rh2BG023100
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004831
Physical Location & Seq
Forward (+)
1557505 .. 1562020
4516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG023100.1

Sequence Viewer

Length: 939 bp
ATGAACAAATACGAACGTGTCCGGACGCTTTCCAAGACTGGGAGGTACGGCGAAGTCTACTCCGTGAGGAACAAGCAGACGAATGGGTATTTCATCGCCCAAGAATTCAATCGGAGAGGCCTTGAAGTTGCCCGGCGCGAATGCGGGTTCCTGGACGAGCTATCCGGGTCGGGTCATGTTATCAAGAAAGTGGAGGAAATTCCGAACTCCGGTGAGCGACTCGCCGTCACGGTGGTCTACGAAGCTCTCGCCGCGACAAGTCTGAGGCGGTTCTTGGACGCCAACCCCGAACAGCCTCTGCCGCTCCGGGCCGTCAAATCGATCACATACCAAACCTTGAAGGGCTTGGCCGACGCTCACAGCCTGGGCATCTTTCACCGTGACATCCGCCCCAGTAAGATCGCCGTCGCGGTCCCCGACGACAGGGGCGGAGCTGTGTTACGGGACGCCAGAAGGGTGGGTCCACCGGAACCCGTCACTCCGACTAGAGACATCGAAAAGGTGAATCTTTACTACCAGTCGCCGGAGCAGCTCATGGGCTCCACAAGCTACGGCGCCGCCGTCGACATGTGGGCGGTCGGATGCATTCTTGCTGAGTTGGCAGATAGGGGCAGGGTTTTGTTCCCTGGAATGAACCTTATCGATTCAAACAGGACACTAATTCAACCCAGGGAGCAGGATACCGAGACACAGTATCTAGGTCAGCTCTACAAGATTTTCAGTATACGCGGTACTCCTCGCCTTCACCTTCTACCAGCAGATGTAGCTGGCTTTCTGGACTGGGAGACTGTCCCTCGGTTTCGCAGAGCCAACCTCCTCCGACGTTTCCCGATGCTTGGTGTTTCTGGCAATCGGCTCCTGGCGGAAATCATGGAGAATGCAGCTTTTAGGGATAGCCCTGAATTAGAGGCGAAACTACCTCATATGAGGCAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000307 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010033 GO:0010103 GO:0010154 GO:0010374 GO:0010376 GO:0010389 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0030332 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0040007 GO:0042023 GO:0042127 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044786 GO:0044843 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051239 GO:0051716 GO:0051726 GO:0060255 GO:0060560 GO:0061458 GO:0061695 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097472 GO:0099402 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901990 GO:1902494 GO:1902554 GO:1902749 GO:1902806 GO:1902911 GO:1903047 GO:1990234 GO:2000026 GO:2000037 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

312

Amino Acids

35.16

Weight (kDa)

9.41

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 13 - 210 7.3e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 59 - 239 3.2e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 554
AccBSI CCGCTC 1 cut(s) 304
AccI GTMKAC 4 cut(s) 57, 237, 564, 724
AccII CGCG 4 cut(s) 138, 254, 410, 729
AccIII TCCGGA 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 2 cut(s) 104, 198
AcyI GRCGYC 3 cut(s) 279, 447, 555
AfaI GTAC 2 cut(s) 47, 733
AfiI CCNNNNNNNGG 5 cut(s) 39, 209, 423, 523, 836
AflIII ACRYGT 2 cut(s) 16, 567
AgsI TTSAA 5 cut(s) 109, 125, 340, 648, 665
AhdI GACNNNNNGTC 1 cut(s) 224
AjnI CCWGG 5 cut(s) 150, 363, 625, 668, 858
AluBI AGCT 9 cut(s) 160, 245, 434, 532, 549, 706, 767, 884, 934
AluI AGCT 9 cut(s) 160, 245, 434, 532, 549, 706, 767, 884, 934
Alw26I GTCTC 3 cut(s) 483, 680, 779
AlwNI CAGNNNCTG 1 cut(s) 298
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 3 cut(s) 118, 309, 348
ApeKI GCWGC 3 cut(s) 529, 881, 931
ApoI RAATTY 2 cut(s) 104, 198
AspLEI GCGC 2 cut(s) 138, 557
AspS9I GGNCC 3 cut(s) 309, 412, 461
AsuC2I CCSGG 3 cut(s) 133, 166, 308
AsuHPI GGTGA 4 cut(s) 224, 368, 514, 737
AvaII GGWCC 2 cut(s) 412, 461
BanI GGYRCC 1 cut(s) 554
BanII GRGCYC 1 cut(s) 542
BbvI GCAGC 2 cut(s) 541, 893
BceAI ACGGC 6 cut(s) 64, 209, 296, 389, 545, 568
BciT130I CCWGG 5 cut(s) 152, 365, 627, 670, 860
BciVI GTATCC 1 cut(s) 673
BcnI CCSGG 3 cut(s) 133, 166, 308
BcoDI GTCTC 3 cut(s) 483, 680, 779
BfaI CTAG 2 cut(s) 486, 698
BfoI RGCGCY 1 cut(s) 558
BfuI GTATCC 1 cut(s) 673
BisI GCNGC 6 cut(s) 252, 302, 530, 558, 882, 932
BlsI GCNGC 6 cut(s) 253, 303, 531, 559, 883, 933
Bme1390I CCNGG 8 cut(s) 133, 152, 166, 308, 365, 627, 670, 860
Bme18I GGWCC 2 cut(s) 412, 461
BmeRI GACNNNNNGTC 1 cut(s) 224
BmgT120I GGNCC 3 cut(s) 309, 412, 461
BmiI GGNNCC 7 cut(s) 149, 414, 462, 471, 541, 556, 857
BmrFI CCNGG 8 cut(s) 133, 152, 166, 308, 365, 627, 670, 860
BmrI ACTGGG 3 cut(s) 48, 387, 790
BmsI GCATC 3 cut(s) 378, 572, 822
BmuI ACTGGG 3 cut(s) 48, 387, 790
BplI GAGNNNNNCTC 2 cut(s) 798, 830
BpuMI CCSGG 3 cut(s) 133, 166, 308
Bsa29I ATCGAT 2 cut(s) 320, 642
BsaHI GRCGYC 3 cut(s) 279, 447, 555
BsaJI CCNNGG 5 cut(s) 364, 625, 668, 669, 794
BsaWI WCCGGW 3 cut(s) 21, 209, 466
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 209, 423, 523, 836
Bse1I ACTGG 4 cut(s) 43, 393, 517, 785
BseAI TCCGGA 1 cut(s) 21
BseBI CCWGG 5 cut(s) 152, 365, 627, 670, 860
BseCI ATCGAT 2 cut(s) 320, 642
BseDI CCNNGG 5 cut(s) 364, 625, 668, 669, 794
BseGI GGATG 2 cut(s) 384, 587
BseLI CCNNNNNNNGG 5 cut(s) 39, 209, 423, 523, 836
BseMII CTCAG 2 cut(s) 254, 585
BseNI ACTGG 4 cut(s) 43, 393, 517, 785
BseRI GAGGAG 2 cut(s) 726, 806
BseXI GCAGC 2 cut(s) 541, 893
Bsh1236I CGCG 4 cut(s) 138, 254, 410, 729
Bsh1285I CGRYCG 1 cut(s) 579
BshFI GGCC 3 cut(s) 120, 311, 350
BshNI GGYRCC 1 cut(s) 554
BshVI ATCGAT 2 cut(s) 320, 642
BsiEI CGRYCG 1 cut(s) 579
BsiSI CCGG 7 cut(s) 22, 133, 165, 210, 307, 467, 524
BslFI GGGAC 3 cut(s) 398, 458, 776
BslI CCNNNNNNNGG 5 cut(s) 39, 209, 423, 523, 836
BsmAI GTCTC 3 cut(s) 483, 680, 779
BsmFI GGGAC 3 cut(s) 398, 458, 776
BsmI GAATGC 3 cut(s) 146, 585, 883
BsnI GGCC 3 cut(s) 120, 311, 350
Bsp1286I GDGCHC 1 cut(s) 542
Bsp13I TCCGGA 1 cut(s) 21
Bsp143I GATC 2 cut(s) 321, 399
BspANI GGCC 3 cut(s) 120, 311, 350
BspCNI CTCAG 2 cut(s) 255, 586
BspDI ATCGAT 2 cut(s) 320, 642
BspEI TCCGGA 1 cut(s) 21
BspFNI CGCG 4 cut(s) 138, 254, 410, 729
BspLI GGNNCC 7 cut(s) 149, 414, 462, 471, 541, 556, 857
BspT107I GGYRCC 1 cut(s) 554
BsrBI CCGCTC 1 cut(s) 304
BsrI ACTGG 4 cut(s) 43, 393, 517, 785
BssECI CCNNGG 5 cut(s) 364, 625, 668, 669, 794
BssMI GATC 2 cut(s) 321, 399
BssNAI GTATAC 1 cut(s) 725
BssNI GRCGYC 3 cut(s) 279, 447, 555
Bst1107I GTATAC 1 cut(s) 725
Bst2UI CCWGG 5 cut(s) 152, 365, 627, 670, 860
Bst4CI ACNGT 4 cut(s) 232, 380, 693, 790
BstACI GRCGYC 3 cut(s) 279, 447, 555
BstC8I GCNNGC 1 cut(s) 769
BstDEI CTNAG 2 cut(s) 263, 594
BstF5I GGATG 2 cut(s) 384, 587
BstFNI CGCG 4 cut(s) 138, 254, 410, 729
BstH2I RGCGCY 1 cut(s) 558
BstHHI GCGC 2 cut(s) 138, 557
BstKTI GATC 2 cut(s) 324, 402
BstMAI GTCTC 3 cut(s) 483, 680, 779
BstMBI GATC 2 cut(s) 321, 399
BstMCI CGRYCG 1 cut(s) 579
BstMWI GCNNNNNNNGC 6 cut(s) 251, 301, 529, 546, 599, 764
BstNI CCWGG 5 cut(s) 152, 365, 627, 670, 860
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 8 cut(s) 131, 150, 164, 306, 363, 625, 668, 858
BstUI CGCG 4 cut(s) 138, 254, 410, 729
BstV1I GCAGC 2 cut(s) 541, 893
BstXI CCANNNNNNTGG 1 cut(s) 457
BstZ17I GTATAC 1 cut(s) 725
Bsu15I ATCGAT 2 cut(s) 320, 642
BsuI GTATCC 1 cut(s) 673
BsuRI GGCC 3 cut(s) 120, 311, 350
BsuTUI ATCGAT 2 cut(s) 320, 642
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 2 cut(s) 384, 587
Cac8I GCNNGC 1 cut(s) 769
CaiI CAGNNNCTG 1 cut(s) 298
CfoI GCGC 2 cut(s) 138, 557
Cfr13I GGNCC 3 cut(s) 309, 412, 461
ClaI ATCGAT 2 cut(s) 320, 642
CseI GACGC 4 cut(s) 34, 287, 362, 455
Csp6I GTAC 2 cut(s) 46, 732
CviAII CATG 4 cut(s) 176, 535, 568, 871
CviQI GTAC 2 cut(s) 46, 732
DdeI CTNAG 2 cut(s) 263, 594
DinI GGCGCC 1 cut(s) 556
DpnI GATC 2 cut(s) 323, 401
DpnII GATC 2 cut(s) 321, 399
DriI GACNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 348
Eam1105I GACNNNNNGTC 1 cut(s) 224
EciI GGCGGA 3 cut(s) 377, 444, 878
Eco147I AGGCCT 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 542
Eco47I GGWCC 2 cut(s) 412, 461
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 5 cut(s) 150, 363, 625, 668, 858
EcoT22I ATGCAT 1 cut(s) 587
EcoT38I GRGCYC 1 cut(s) 542
EgeI GGCGCC 1 cut(s) 556
EheI GGCGCC 1 cut(s) 556
FaeI CATG 4 cut(s) 179, 538, 571, 874
FaiI YATR 8 cut(s) 177, 328, 536, 569, 725, 872, 924, 926
FaqI GGGAC 3 cut(s) 398, 458, 776
FatI CATG 4 cut(s) 175, 534, 567, 870
FauI CCCGC 1 cut(s) 137
FauNDI CATATG 1 cut(s) 924
FblI GTMKAC 4 cut(s) 57, 237, 564, 724
Fnu4HI GCNGC 6 cut(s) 252, 302, 530, 558, 882, 932
FokI GGATG 2 cut(s) 371, 594
FriOI GRGCYC 1 cut(s) 542
Fsp4HI GCNGC 6 cut(s) 252, 302, 530, 558, 882, 932
FspBI CTAG 2 cut(s) 486, 698
GlaI GCGC 2 cut(s) 137, 556
GluI GCNGC 6 cut(s) 252, 302, 530, 558, 882, 932
HaeII RGCGCY 1 cut(s) 558
HaeIII GGCC 3 cut(s) 120, 311, 350
HapII CCGG 7 cut(s) 22, 133, 165, 210, 307, 467, 524
HgaI GACGC 4 cut(s) 34, 287, 362, 455
HhaI GCGC 2 cut(s) 138, 557
Hin1I GRCGYC 3 cut(s) 279, 447, 555
Hin1II CATG 4 cut(s) 179, 538, 571, 874
Hin6I GCGC 2 cut(s) 136, 555
HinP1I GCGC 2 cut(s) 136, 555
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 3 cut(s) 219, 505, 644
HpaII CCGG 7 cut(s) 22, 133, 165, 210, 307, 467, 524
HphI GGTGA 4 cut(s) 224, 368, 514, 737
Hpy166II GTNNAC 5 cut(s) 58, 238, 464, 565, 725
Hpy188I TCNGA 6 cut(s) 114, 204, 264, 483, 581, 821
Hpy188III TCNNGA 4 cut(s) 22, 184, 776, 829
Hpy8I GTNNAC 5 cut(s) 58, 238, 464, 565, 725
Hpy99I CGWCG 5 cut(s) 356, 410, 422, 566, 825
HpyAV CCTTC 4 cut(s) 334, 447, 752, 758
HpyCH4III ACNGT 4 cut(s) 232, 380, 693, 790
HpyCH4IV ACGT 2 cut(s) 16, 823
HpyCH4V TGCA 2 cut(s) 585, 881
HpyF10VI GCNNNNNNNGC 6 cut(s) 251, 301, 529, 546, 599, 764
HpyF3I CTNAG 2 cut(s) 263, 594
HpySE526I ACGT 2 cut(s) 16, 823
Hsp92I GRCGYC 3 cut(s) 279, 447, 555
Hsp92II CATG 4 cut(s) 179, 538, 571, 874
HspAI GCGC 2 cut(s) 136, 555
KasI GGCGCC 1 cut(s) 554
Kpn2I TCCGGA 1 cut(s) 21
Kzo9I GATC 2 cut(s) 321, 399
LmnI GCTCC 6 cut(s) 309, 431, 526, 545, 673, 861
Lsp1109I GCAGC 2 cut(s) 541, 893
LweI GCATC 3 cut(s) 378, 572, 822
MaeI CTAG 2 cut(s) 486, 698
MaeII ACGT 2 cut(s) 16, 823
MaeIII GTNAC 4 cut(s) 226, 380, 438, 475
MalI GATC 2 cut(s) 323, 401
MbiI CCGCTC 1 cut(s) 304
MboI GATC 2 cut(s) 321, 399
MhlI GDGCHC 1 cut(s) 542
MluCI AATT 4 cut(s) 104, 198, 660, 902
Mly113I GGCGCC 1 cut(s) 555
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 3 cut(s) 506, 559, 844
Mph1103I ATGCAT 1 cut(s) 587
MroI TCCGGA 1 cut(s) 21
MspI CCGG 7 cut(s) 22, 133, 165, 210, 307, 467, 524
MspR9I CCNGG 8 cut(s) 133, 152, 166, 308, 365, 627, 670, 860
Mva1269I GAATGC 3 cut(s) 146, 585, 883
MvaI CCWGG 5 cut(s) 152, 365, 627, 670, 860
MvnI CGCG 4 cut(s) 138, 254, 410, 729
MwoI GCNNNNNNNGC 6 cut(s) 251, 301, 529, 546, 599, 764
NarI GGCGCC 1 cut(s) 555
NciI CCSGG 3 cut(s) 133, 166, 308
NdeI CATATG 1 cut(s) 924
NdeII GATC 2 cut(s) 321, 399
NlaIII CATG 4 cut(s) 179, 538, 571, 874
NlaIV GGNNCC 7 cut(s) 149, 414, 462, 471, 541, 556, 857
NmuCI GTSAC 3 cut(s) 226, 380, 475
NsiI ATGCAT 1 cut(s) 587
NspI RCATGY 1 cut(s) 571
PasI CCCWGGG 1 cut(s) 669
PceI AGGCCT 1 cut(s) 120
PciI ACATGT 1 cut(s) 567
PcsI WCGNNNNNNNCGW 3 cut(s) 285, 414, 558
PctI GAATGC 3 cut(s) 146, 585, 883
PfeI GAWTC 2 cut(s) 505, 644
PfoI TCCNGGA 1 cut(s) 150
PkrI GCNGC 6 cut(s) 253, 303, 531, 559, 883, 933
PleI GAGTC 1 cut(s) 213
PluTI GGCGCC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 213
PscI ACATGT 1 cut(s) 567
Psp6I CCWGG 5 cut(s) 150, 363, 625, 668, 858
PspGI CCWGG 5 cut(s) 150, 363, 625, 668, 858
PspN4I GGNNCC 7 cut(s) 149, 414, 462, 471, 541, 556, 857
PspPI GGNCC 3 cut(s) 309, 412, 461
PstNI CAGNNNCTG 1 cut(s) 298
RsaI GTAC 2 cut(s) 47, 733
RsaNI GTAC 2 cut(s) 46, 732
SalI GTCGAC 1 cut(s) 563
SatI GCNGC 6 cut(s) 252, 302, 530, 558, 882, 932
Sau3AI GATC 2 cut(s) 321, 399
Sau96I GGNCC 3 cut(s) 309, 412, 461
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 8 cut(s) 133, 152, 166, 308, 365, 627, 670, 860
SduI GDGCHC 1 cut(s) 542
SfaNI GCATC 3 cut(s) 378, 572, 822
SfoI GGCGCC 1 cut(s) 556
SinI GGWCC 2 cut(s) 412, 461
Sse9I AATT 4 cut(s) 104, 198, 660, 902
SseBI AGGCCT 1 cut(s) 120
SspDI GGCGCC 1 cut(s) 554
SspMI CTAG 2 cut(s) 486, 698
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 8 cut(s) 131, 150, 164, 306, 363, 625, 668, 858
TaaI ACNGT 4 cut(s) 232, 380, 693, 790
TaiI ACGT 2 cut(s) 19, 826
TaqI TCGA 4 cut(s) 320, 495, 564, 642
TasI AATT 4 cut(s) 104, 198, 660, 902
TauI GCSGC 3 cut(s) 254, 304, 560
TfiI GAWTC 2 cut(s) 505, 644
TseFI GTSAC 3 cut(s) 226, 380, 475
TseI GCWGC 3 cut(s) 529, 881, 931
Tsp45I GTSAC 3 cut(s) 226, 380, 475
TspDTI ATGAA 3 cut(s) 17, 82, 647
TspGWI ACGGA 1 cut(s) 52
VpaK11BI GGWCC 2 cut(s) 412, 461
XapI RAATTY 2 cut(s) 104, 198
XceI RCATGY 1 cut(s) 571
XmiI GTMKAC 4 cut(s) 57, 237, 564, 724
XspI CTAG 2 cut(s) 486, 698
Zsp2I ATGCAT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.