Rh2DG023000

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004831
Physical Location & Seq
Reverse (-)
1356739 .. 1358603
1865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG023000.1

Sequence Viewer

Length: 1188 bp
ATGCGTGGGACTTGTTCCAATGTTGATGATTCCCCTCAAGAACAACCATCTGAGCAGCCAATAATATGTGAGTGTTCCAATGAGGAAAATTGTGAATGTAGTAGAGGGATGACTTCTGCTGCACATTTTATAAAACACCTTATTCCGGGTGAAAGTCAAGGGCCTCAGCTGAATAATGCCGGTGTTGGATTGCTTGCCAATTTTGAATCCGAGAGGGTCAATGCCCATCTTTTATCTCATCCTGCATCTTCATCTTCATCCTCATCCCAAAGATCACATTCACATCAAGCGCATACAAGCAGCCAGGTATCAAACAAGGCAGAGTGTACTTCATCAATGGTCAATCATAACTCGTCGCAAAGACTAATGGTCCGAAGGAACTGGCATTCTAATTCAGAAGCCACAGTAAGAGGCCTAACAAGTTGCATTCCCAACAGTACTCCAAACCTTATTAACTCTGGAAATTGCACCACATTGCTTTTCGGATCCACTCCTCTTTGCCGATTCTCTCTTTCTCAAGGTCTTTTGGGGGTGCCTTCGAATCTTTTCCACTCTGTAATGCCTGCCATTCAGCTTCCCCACGCCAGTGGAGATTCAACATCTCAAATAGAAGAAGCTCCTGCTACTTTTGATGATAGTGGATTCCCTAGTGAGAACCAAATAATTGATATATGGAGGCAACTCCTCATGGAATCGACTCTATATATACATAGGCCGGCTGATACCTTGTTAGAAGGTTCAAGTTCCGTCTTTAGGGTAACGGCTGACAATGGATCAGTGGACTTCAGACTTGTAACAGAGAACGCTGAAGTTGTTAACCAATCACTAGTGTACTTTTCTATTGAAATGCGCAATCGCTTTGGAGGCATGAGGCTTGATGTGGATAACATGTCATATGAGGAACTATTGGCTTTAGAAGATCATATAGGGTATGTCAGCACAGGATTATCGGAGGAAGCAGCTGTTGCATCTCTCAAATGCAGTAATTATTTTCTGCTTGCTGATGAAAATGCTCAGAAAGAGTTTTGCAGCATCTGCCAGGAGGATTTTATAGAGGAAGATGAGCTAGGAACATTGGATTGCGGACACAGCTTCCACATTTGTTGCATCAAACAATGGCTAGGATATAAAAACGTGTGTCCTATATGCAAATCTCCTGCCTTATCTACTACGTTCTCACTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

395

Amino Acids

43.24

Weight (kDa)

4.94

Isoelectric Point (pI)

55.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 340 - 384 6.6e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 343 - 384 3.7e-12 Ring finger domain
zf-C3HC4_2 PF13923 343 - 383 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 343 - 383 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 343 - 381 5.4e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
Acc16I TGCGCA 1 cut(s) 851
AccB1I GGYRCC 1 cut(s) 532
AciI CCGC 1 cut(s) 1083
AclWI GGATC 3 cut(s) 480, 493, 781
AcuI CTGAAG 2 cut(s) 769, 828
AfaI GTAC 3 cut(s) 328, 439, 833
AfiI CCNNNNNNNGG 2 cut(s) 145, 753
AflIII ACRYGT 2 cut(s) 888, 1134
AgsI TTSAA 4 cut(s) 206, 597, 741, 845
AhdI GACNNNNNGTC 1 cut(s) 368
AhlI ACTAGT 1 cut(s) 826
AjnI CCWGG 2 cut(s) 303, 1038
AleI CACNNNNGTG 1 cut(s) 585
AluBI AGCT 6 cut(s) 169, 574, 617, 962, 1066, 1092
AluI AGCT 6 cut(s) 169, 574, 617, 962, 1066, 1092
AlwI GGATC 3 cut(s) 480, 493, 781
AlwNI CAGNNNCTG 1 cut(s) 1035
AoxI GGCC 3 cut(s) 161, 412, 713
ApeKI GCWGC 5 cut(s) 55, 119, 300, 959, 1029
Asp700I GAANNNNTTC 1 cut(s) 545
AspLEI GCGC 2 cut(s) 292, 852
AspS9I GGNCC 2 cut(s) 161, 370
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 161
AsuII TTCGAA 1 cut(s) 539
AvaII GGWCC 1 cut(s) 370
BamHI GGATCC 1 cut(s) 485
BanI GGYRCC 1 cut(s) 532
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 5 cut(s) 67, 106, 312, 971, 1041
BccI CCATC 2 cut(s) 55, 234
BceAI ACGGC 1 cut(s) 777
BciT130I CCWGG 2 cut(s) 305, 1040
BcnI CCSGG 1 cut(s) 147
BcuI ACTAGT 1 cut(s) 826
BfaI CTAG 4 cut(s) 648, 827, 1067, 1121
BisI GCNGC 5 cut(s) 56, 120, 301, 960, 1030
BlsI GCNGC 5 cut(s) 57, 121, 302, 961, 1031
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 3 cut(s) 147, 305, 1040
Bme18I GGWCC 1 cut(s) 370
BmeRI GACNNNNNGTC 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 161, 370
BmiI GGNNCC 2 cut(s) 487, 534
BmrFI CCNGG 3 cut(s) 147, 305, 1040
BmsI GCATC 4 cut(s) 254, 977, 1041, 1116
Bpu10I CCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 539
BpuEI CTTGAG 2 cut(s) 21, 501
BpuMI CCSGG 1 cut(s) 147
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 753
Bse118I RCCGGY 2 cut(s) 179, 715
Bse1I ACTGG 2 cut(s) 386, 585
Bse3DI GCAATG 1 cut(s) 473
BseBI CCWGG 2 cut(s) 305, 1040
BseGI GGATG 4 cut(s) 114, 238, 257, 263
BseLI CCNNNNNNNGG 2 cut(s) 145, 753
BseMI GCAATG 1 cut(s) 473
BseMII CTCAG 3 cut(s) 42, 179, 1028
BseNI ACTGG 2 cut(s) 386, 585
BseRI GAGGAG 2 cut(s) 483, 674
BseXI GCAGC 5 cut(s) 67, 106, 312, 971, 1041
BsgI GTGCAG 1 cut(s) 105
BshFI GGCC 3 cut(s) 163, 414, 715
BshNI GGYRCC 1 cut(s) 532
BsiSI CCGG 3 cut(s) 146, 180, 716
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 145, 753
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 2 cut(s) 385, 426
BsnI GGCC 3 cut(s) 163, 414, 715
Bsp119I TTCGAA 1 cut(s) 539
Bsp143I GATC 4 cut(s) 272, 485, 773, 919
BspACI CCGC 1 cut(s) 1083
BspANI GGCC 3 cut(s) 163, 414, 715
BspCNI CTCAG 3 cut(s) 43, 178, 1027
BspLI GGNNCC 2 cut(s) 487, 534
BspPI GGATC 3 cut(s) 480, 493, 781
BspT104I TTCGAA 1 cut(s) 539
BspT107I GGYRCC 1 cut(s) 532
BsrDI GCAATG 1 cut(s) 473
BsrFI RCCGGY 2 cut(s) 179, 715
BsrI ACTGG 2 cut(s) 386, 585
BssAI RCCGGY 2 cut(s) 179, 715
BssMI GATC 4 cut(s) 272, 485, 773, 919
Bst2UI CCWGG 2 cut(s) 305, 1040
Bst4CI ACNGT 2 cut(s) 406, 437
BstAPI GCANNNNNTGC 2 cut(s) 965, 1035
BstBI TTCGAA 1 cut(s) 539
BstC8I GCNNGC 4 cut(s) 195, 564, 717, 999
BstDEI CTNAG 3 cut(s) 51, 165, 1014
BstF5I GGATG 4 cut(s) 114, 238, 257, 263
BstHHI GCGC 2 cut(s) 292, 852
BstKTI GATC 4 cut(s) 275, 488, 776, 922
BstMBI GATC 4 cut(s) 272, 485, 773, 919
BstMWI GCNNNNNNNGC 4 cut(s) 864, 965, 1035, 1089
BstNI CCWGG 2 cut(s) 305, 1040
BstNSI RCATGY 1 cut(s) 892
BstSCI CCNGG 3 cut(s) 145, 303, 1038
BstV1I GCAGC 5 cut(s) 67, 106, 312, 971, 1041
BstX2I RGATCY 1 cut(s) 485
BstXI CCANNNNNNTGG 1 cut(s) 587
BstYI RGATCY 1 cut(s) 485
BsuRI GGCC 3 cut(s) 163, 414, 715
BtsCI GGATG 4 cut(s) 114, 238, 257, 263
BtsIMutI CAGTG 2 cut(s) 592, 783
Cac8I GCNNGC 4 cut(s) 195, 564, 717, 999
CaiI CAGNNNCTG 1 cut(s) 1035
CfoI GCGC 2 cut(s) 292, 852
Cfr10I RCCGGY 2 cut(s) 179, 715
Cfr13I GGNCC 2 cut(s) 161, 370
Csp6I GTAC 3 cut(s) 327, 438, 832
CspCI CAANNNNNGTGG 2 cut(s) 1085, 1120
CviAII CATG 3 cut(s) 688, 868, 889
CviQI GTAC 3 cut(s) 327, 438, 832
DdeI CTNAG 3 cut(s) 51, 165, 1014
DpnI GATC 4 cut(s) 274, 487, 775, 921
DpnII GATC 4 cut(s) 272, 485, 773, 919
DriI GACNNNNNGTC 1 cut(s) 368
Eam1105I GACNNNNNGTC 1 cut(s) 368
Eco147I AGGCCT 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 370
Eco57I CTGAAG 2 cut(s) 769, 828
EcoO109I RGGNCCY 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 303, 1038
FaeI CATG 3 cut(s) 691, 871, 892
FaqI GGGAC 1 cut(s) 22
FatI CATG 3 cut(s) 687, 867, 888
FauNDI CATATG 1 cut(s) 895
Fnu4HI GCNGC 5 cut(s) 56, 120, 301, 960, 1030
FokI GGATG 4 cut(s) 121, 225, 244, 250
Fsp4HI GCNGC 5 cut(s) 56, 120, 301, 960, 1030
FspBI CTAG 4 cut(s) 648, 827, 1067, 1121
FspI TGCGCA 1 cut(s) 851
GlaI GCGC 2 cut(s) 291, 851
GluI GCNGC 5 cut(s) 56, 120, 301, 960, 1030
HaeIII GGCC 3 cut(s) 163, 414, 715
HapII CCGG 3 cut(s) 146, 180, 716
HhaI GCGC 2 cut(s) 292, 852
Hin1II CATG 3 cut(s) 691, 871, 892
Hin6I GCGC 2 cut(s) 290, 850
HinP1I GCGC 2 cut(s) 290, 850
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HinfI GANTC 8 cut(s) 29, 206, 504, 541, 593, 642, 692, 697
HpaI GTTAAC 1 cut(s) 817
HpaII CCGG 3 cut(s) 146, 180, 716
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 4 cut(s) 327, 781, 817, 832
Hpy188I TCNGA 8 cut(s) 52, 211, 374, 397, 485, 788, 952, 1017
Hpy188III TCNNGA 2 cut(s) 38, 459
Hpy8I GTNNAC 4 cut(s) 327, 781, 817, 832
Hpy99I CGWCG 1 cut(s) 358
HpyAV CCTTC 3 cut(s) 369, 546, 728
HpyCH4III ACNGT 2 cut(s) 406, 437
HpyCH4IV ACGT 2 cut(s) 1134, 1172
HpyCH4V TGCA 9 cut(s) 122, 245, 426, 468, 968, 981, 1029, 1107, 1149
HpyF10VI GCNNNNNNNGC 4 cut(s) 864, 965, 1035, 1089
HpyF3I CTNAG 3 cut(s) 51, 165, 1014
HpySE526I ACGT 2 cut(s) 1134, 1172
Hsp92II CATG 3 cut(s) 691, 871, 892
HspAI GCGC 2 cut(s) 290, 850
KroI GCCGGC 1 cut(s) 715
KroNI GCCGGC 1 cut(s) 717
KspAI GTTAAC 1 cut(s) 817
Kzo9I GATC 4 cut(s) 272, 485, 773, 919
LmnI GCTCC 1 cut(s) 622
Lsp1109I GCAGC 5 cut(s) 67, 106, 312, 971, 1041
LweI GCATC 4 cut(s) 254, 977, 1041, 1116
MaeI CTAG 4 cut(s) 648, 827, 1067, 1121
MaeII ACGT 2 cut(s) 1134, 1172
MaeIII GTNAC 2 cut(s) 757, 793
MalI GATC 4 cut(s) 274, 487, 775, 921
MboI GATC 4 cut(s) 272, 485, 773, 919
MboII GAAGA 5 cut(s) 240, 246, 623, 929, 1070
MflI RGATCY 1 cut(s) 485
MluCI AATT 6 cut(s) 88, 199, 391, 463, 663, 985
MlyI GAGTC 1 cut(s) 691
MmeI TCCRAC 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 715
MroXI GAANNNNTTC 1 cut(s) 545
MseI TTAA 3 cut(s) 453, 816, 1186
MslI CAYNNNNRTG 1 cut(s) 585
MspA1I CMGCKG 2 cut(s) 169, 962
MspI CCGG 3 cut(s) 146, 180, 716
MspR9I CCNGG 3 cut(s) 147, 305, 1040
Mva1269I GAATGC 2 cut(s) 385, 426
MvaI CCWGG 2 cut(s) 305, 1040
MwoI GCNNNNNNNGC 4 cut(s) 864, 965, 1035, 1089
NaeI GCCGGC 1 cut(s) 717
NciI CCSGG 1 cut(s) 147
NdeI CATATG 1 cut(s) 895
NdeII GATC 4 cut(s) 272, 485, 773, 919
NgoMIV GCCGGC 1 cut(s) 715
NlaIII CATG 3 cut(s) 691, 871, 892
NlaIV GGNNCC 2 cut(s) 487, 534
NsbI TGCGCA 1 cut(s) 851
NspI RCATGY 1 cut(s) 892
NspV TTCGAA 1 cut(s) 539
OliI CACNNNNGTG 1 cut(s) 585
PceI AGGCCT 1 cut(s) 414
PciI ACATGT 1 cut(s) 888
PctI GAATGC 2 cut(s) 385, 426
PdiI GCCGGC 1 cut(s) 717
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 7 cut(s) 29, 206, 504, 541, 593, 642, 692
PkrI GCNGC 5 cut(s) 57, 121, 302, 961, 1031
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
PscI ACATGT 1 cut(s) 888
PsiI TTATAA 1 cut(s) 131
Psp6I CCWGG 2 cut(s) 303, 1038
PspGI CCWGG 2 cut(s) 303, 1038
PspN4I GGNNCC 2 cut(s) 487, 534
PspPI GGNCC 2 cut(s) 161, 370
PstNI CAGNNNCTG 1 cut(s) 1035
PsuI RGATCY 1 cut(s) 485
PvuII CAGCTG 2 cut(s) 169, 962
RsaI GTAC 3 cut(s) 328, 439, 833
RsaNI GTAC 3 cut(s) 327, 438, 832
RseI CAYNNNNRTG 1 cut(s) 585
SaqAI TTAA 3 cut(s) 453, 816, 1186
SatI GCNGC 5 cut(s) 56, 120, 301, 960, 1030
Sau3AI GATC 4 cut(s) 272, 485, 773, 919
Sau96I GGNCC 2 cut(s) 161, 370
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 1 cut(s) 691
ScrFI CCNGG 3 cut(s) 147, 305, 1040
SfaNI GCATC 4 cut(s) 254, 977, 1041, 1116
SfuI TTCGAA 1 cut(s) 539
SinI GGWCC 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 2 cut(s) 36, 516
SmoI CTYRAG 2 cut(s) 36, 516
SpeI ACTAGT 1 cut(s) 826
Sse9I AATT 6 cut(s) 88, 199, 391, 463, 663, 985
SseBI AGGCCT 1 cut(s) 414
SsiI CCGC 1 cut(s) 1083
SspMI CTAG 4 cut(s) 648, 827, 1067, 1121
StuI AGGCCT 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 145, 303, 1038
TaaI ACNGT 2 cut(s) 406, 437
TaiI ACGT 2 cut(s) 1137, 1175
TaqI TCGA 2 cut(s) 539, 695
TasI AATT 6 cut(s) 88, 199, 391, 463, 663, 985
TatI WGTACW 3 cut(s) 326, 437, 831
TfiI GAWTC 7 cut(s) 29, 206, 504, 541, 593, 642, 692
Tru1I TTAA 3 cut(s) 453, 816, 1186
Tru9I TTAA 3 cut(s) 453, 816, 1186
TscAI CASTG 2 cut(s) 592, 783
TseI GCWGC 5 cut(s) 55, 119, 300, 959, 1029
TspDTI ATGAA 4 cut(s) 240, 246, 321, 1020
TspGWI ACGGA 1 cut(s) 736
TspRI CASTG 2 cut(s) 592, 783
VpaK11BI GGWCC 1 cut(s) 370
XceI RCATGY 1 cut(s) 892
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 4 cut(s) 648, 827, 1067, 1121
ZrmI AGTACT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.