MD02G1016500.v1.1

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004831
Physical Location & Seq
Forward (+)
1246126 .. 1246696
571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1016500.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGAGTCGACTCTATACATGCACAGGCCACCTGATAGCTTGCAGAACGCTGGAGCTACTTACCAACGACCTCTGCACTTCTCGATTGAAATGCGTGATCGTTATGGAGCAATGTGGCTCGATGTGGATAGCATGTCATATGAGGAACTATTGGCTTTAGAAGATCATATAGGGTATGTCAGTACAGGATTATCTGAGGAAGCAGCTGTCACGTCTCTCAAACGCAGCAATTACTTTGTGTTTAAAGAAGAAAATGTTCAGAAAGACTTCTGCAGCATCGGTCAGGAGGATTTTGTAGAGGAGGATGAGCTAGGAACATTGGACTGTGGACACGGCTTCCACATTTCTTGCATTAAGCAATGGCTAGGGCATAAAAATGTTTGTCCTATGTGCAAATCTCCTGGTTTAAGTTCTAAGCTACCCTCATTTTCGCACATTCAAAGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

150

Amino Acids

16.93

Weight (kDa)

5.01

Isoelectric Point (pI)

67.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 91 - 132 3.1e-12 Ring finger domain
zf-C3HC4 PF00097 91 - 131 7.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 104 - 131 5.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 108 - 132 8.2e-10 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 8
AfaI GTAC 1 cut(s) 184
AgsI TTSAA 2 cut(s) 89, 440
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 5 cut(s) 39, 56, 206, 310, 418
AluI AGCT 5 cut(s) 39, 56, 206, 310, 418
Alw26I GTCTC 1 cut(s) 219
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 3 cut(s) 203, 225, 273
Asp700I GAANNNNTTC 2 cut(s) 255, 266
BbvI GCAGC 3 cut(s) 215, 237, 285
BceAI ACGGC 1 cut(s) 349
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 219
BfaI CTAG 2 cut(s) 311, 365
BfmI CTRYAG 1 cut(s) 271
BisI GCNGC 3 cut(s) 204, 226, 274
BlsI GCNGC 3 cut(s) 205, 227, 275
Bme1390I CCNGG 1 cut(s) 402
BmgBI CACGTC 1 cut(s) 213
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 1 cut(s) 285
BpmI CTGGAG 1 cut(s) 72
Bse3DI GCAATG 2 cut(s) 117, 365
BseBI CCWGG 1 cut(s) 402
BseGI GGATG 1 cut(s) 310
BseMI GCAATG 2 cut(s) 117, 365
BseMII CTCAG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 314
BseXI GCAGC 3 cut(s) 215, 237, 285
BsgI GTGCAG 1 cut(s) 59
BshFI GGCC 1 cut(s) 28
BsmAI GTCTC 1 cut(s) 219
BsmBI CGTCTC 1 cut(s) 219
BsnI GGCC 1 cut(s) 28
Bsp143I GATC 2 cut(s) 97, 163
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 275
BsrDI GCAATG 2 cut(s) 117, 365
BssMI GATC 2 cut(s) 97, 163
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 195, 414
BstF5I GGATG 1 cut(s) 310
BstKTI GATC 2 cut(s) 100, 166
BstMAI GTCTC 1 cut(s) 219
BstMBI GATC 2 cut(s) 97, 163
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 2 cut(s) 22, 136
BstSCI CCNGG 1 cut(s) 400
BstSFI CTRYAG 1 cut(s) 271
BstV1I GCAGC 3 cut(s) 215, 237, 285
BsuRI GGCC 1 cut(s) 28
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 183
CspCI CAANNNNNGTGG 2 cut(s) 329, 364
CviAII CATG 2 cut(s) 19, 133
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 2 cut(s) 195, 414
DpnI GATC 2 cut(s) 99, 165
DpnII GATC 2 cut(s) 97, 163
DraI TTTAAA 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 400
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 2 cut(s) 22, 136
FatI CATG 2 cut(s) 18, 132
FauNDI CATATG 1 cut(s) 139
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 3 cut(s) 204, 226, 274
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 3 cut(s) 204, 226, 274
FspBI CTAG 2 cut(s) 311, 365
GluI GCNGC 3 cut(s) 204, 226, 274
GsuI CTGGAG 1 cut(s) 72
HaeIII GGCC 1 cut(s) 28
Hin1II CATG 2 cut(s) 22, 136
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 2 cut(s) 5, 10
Hpy166II GTNNAC 2 cut(s) 9, 329
Hpy188I TCNGA 2 cut(s) 196, 261
Hpy188III TCNNGA 2 cut(s) 82, 284
Hpy8I GTNNAC 2 cut(s) 9, 329
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 6 cut(s) 22, 43, 76, 273, 351, 393
HpyF3I CTNAG 2 cut(s) 195, 414
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 2 cut(s) 22, 136
Kzo9I GATC 2 cut(s) 97, 163
LmnI GCTCC 2 cut(s) 53, 107
LpnPI CCDG 7 cut(s) 10, 36, 45, 171, 269, 387, 414
Lsp1109I GCAGC 3 cut(s) 215, 237, 285
LweI GCATC 1 cut(s) 285
MaeI CTAG 2 cut(s) 311, 365
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 208
MalI GATC 2 cut(s) 99, 165
MboI GATC 2 cut(s) 97, 163
MboII GAAGA 2 cut(s) 173, 260
MluCI AATT 1 cut(s) 229
MlyI GAGTC 2 cut(s) 4, 14
MnlI CCTC 7 cut(s) 81, 136, 190, 280, 292, 295, 433
MroXI GAANNNNTTC 2 cut(s) 255, 266
MseI TTAA 3 cut(s) 243, 354, 407
MslI CAYNNNNRTG 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 206
MspR9I CCNGG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 402
NdeI CATATG 1 cut(s) 139
NdeII GATC 2 cut(s) 97, 163
NlaIII CATG 2 cut(s) 22, 136
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 2 cut(s) 22, 136
PdmI GAANNNNTTC 2 cut(s) 255, 266
PkrI GCNGC 3 cut(s) 205, 227, 275
PleI GAGTC 2 cut(s) 4, 13
PpsI GAGTC 2 cut(s) 4, 13
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PstI CTGCAG 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
RseI CAYNNNNRTG 1 cut(s) 375
SalI GTCGAC 1 cut(s) 7
SaqAI TTAA 3 cut(s) 243, 354, 407
SatI GCNGC 3 cut(s) 204, 226, 274
Sau3AI GATC 2 cut(s) 97, 163
SchI GAGTC 2 cut(s) 4, 14
ScrFI CCNGG 1 cut(s) 402
SetI ASST 8 cut(s) 34, 41, 58, 73, 208, 215, 312, 420
SfaNI GCATC 1 cut(s) 285
SfcI CTRYAG 1 cut(s) 271
SmiMI CAYNNNNRTG 1 cut(s) 375
Sse9I AATT 1 cut(s) 229
SspMI CTAG 2 cut(s) 311, 365
StyD4I CCNGG 1 cut(s) 400
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 215
TaqI TCGA 3 cut(s) 8, 83, 120
TaqII GACCGA 1 cut(s) 269
TasI AATT 1 cut(s) 229
TatI WGTACW 1 cut(s) 182
Tru1I TTAA 3 cut(s) 243, 354, 407
Tru9I TTAA 3 cut(s) 243, 354, 407
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 3 cut(s) 203, 225, 273
Tsp45I GTSAC 1 cut(s) 208
XceI RCATGY 2 cut(s) 22, 136
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 2 cut(s) 255, 266
XspI CTAG 2 cut(s) 311, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.