MD15G1161000.v1.1

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004831
Physical Location & Seq
Forward (+)
12093106 .. 12094924
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1161000.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGATGCATGCAACTTGTTCTAATGCTAATCCTCCTCAAGAACCAACATGTTGGTGCTCGAATGCAGAACAATGTGACTGTGGTAAAAGGATGGGGGAAGATGCTGGTTTACTTGCCAATTTTGAATCTGCAGAGAGGGTGAATATTAATAAACACTTTTTATCTCTTCTTACATCTTCGTCCTCGCTGCGAGGATCACAACAAGCTTTAAGACCGGCGATCCTAATTGTTCAAAGGAGCTCACATGATCCGCTTTCAGCAGCCATCGCAAGGAGCATAACAAGTTGCATTGCCAACACTCCAAACCTCAACTTATCTCATACAATACTTAACCAAATTGGAAGAGCTAATACTAATTTTCCAGGTTCACAGAATAATGAGTTCAATTCTGGCACTTGCACCACTTTGATTCTTGGATCCGCTCCTCTTCTTCGCCAGTTTTATCTTTTTCAACGTCTTTTTGGGATGCCTAACAATTTTTTTCGATCTTTATGGCCTCCCCACGTAAGTCGCGTAGATTCTACATTTCTATTAGGTCAGGCTTCTGCGGCTTCTGATGATAACTGGTTCCAAAATGAAACCCGAATTGATCTGTGGAGGCAACTTCTCATGGAGTCGACTCTATACATGCACAGGCCACCCGATAGCTTGTTAGAAGGTCCGACTTCATTTTTTGTTGTAACAGCCAGCCACGGAGTAAACTTCAGTATGAACGCTGAAGCTATTTTCCAACGACCTCTCCACTTCTCGATTGAAATGCGCAATCATTCTGGAGCAATGTGGCCCGATGTGGATAACATGTCATATGAGGAATTATTGGCTTTAGAAGATCGTATAGGGTATGTCAGCACAGGATTATCTGAGGAAGTAGCTGTCAAGTCTCTCAAACGCAGCCATTACTTTGTGTTTACAGAAGAAAATCCTCAGAAAGACTTCTGCAGCATCTGCCAGGAGGATTTTGTAGAAGAGGATGAGCTAGGAACATTGGACTGCGGACACGGCTTCCACATTTCGTGCATTAAGCAATGGCTAGGGTATAAAAATGTGTGTCCTATGTGCAAATCTCCCGGTATAAGTACTACTCTTCCCTCGTTTTCTCACATTCAAAGTCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

372

Amino Acids

41.46

Weight (kDa)

5.6

Isoelectric Point (pI)

56.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 309 - 354 1.3e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 313 - 354 2.1e-13 Ring finger domain
zf-C3HC4 PF00097 313 - 353 1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 313 - 353 6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 313 - 340 1.4e-07 RING-like zinc finger
zf-RING_UBOX PF13445 313 - 351 2.5e-06 RING-type zinc-finger
zf-RING_5 PF14634 313 - 354 3.5e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 761
AccBSI CCGCTC 1 cut(s) 422
AccI GTMKAC 1 cut(s) 617
AccII CGCG 1 cut(s) 513
AciI CCGC 4 cut(s) 251, 420, 548, 993
AclWI GGATC 5 cut(s) 202, 214, 242, 411, 424
AcuI CTGAAG 2 cut(s) 688, 738
AfaI GTAC 1 cut(s) 1078
AfiI CCNNNNNNNGG 1 cut(s) 270
AflIII ACRYGT 2 cut(s) 47, 798
AgsI TTSAA 6 cut(s) 125, 233, 385, 452, 755, 1106
AjnI CCWGG 2 cut(s) 361, 948
AluBI AGCT 7 cut(s) 206, 240, 347, 648, 722, 872, 976
AluI AGCT 7 cut(s) 206, 240, 347, 648, 722, 872, 976
Alw21I GWGCWC 2 cut(s) 59, 242
Alw26I GTCTC 1 cut(s) 885
AlwI GGATC 5 cut(s) 202, 214, 242, 411, 424
AlwNI CAGNNNCTG 1 cut(s) 945
AoxI GGCC 3 cut(s) 494, 635, 782
ApeKI GCWGC 4 cut(s) 187, 260, 891, 939
AseI ATTAAT 1 cut(s) 147
Asp700I GAANNNNTTC 1 cut(s) 932
AspLEI GCGC 1 cut(s) 762
AspS9I GGNCC 2 cut(s) 659, 783
AsuC2I CCSGG 1 cut(s) 1068
AsuHPI GGTGA 1 cut(s) 151
AvaII GGWCC 1 cut(s) 659
BamHI GGATCC 1 cut(s) 416
BanII GRGCYC 1 cut(s) 242
BarI GAAGNNNNNNTAC 2 cut(s) 334, 366
Bbv12I GWGCWC 2 cut(s) 59, 242
BbvI GCAGC 4 cut(s) 174, 272, 903, 951
BccI CCATC 2 cut(s) 85, 272
BceAI ACGGC 1 cut(s) 1015
BcgI CGANNNNNNTGC 2 cut(s) 739, 773
BciT130I CCWGG 2 cut(s) 363, 950
BcnI CCSGG 1 cut(s) 1068
BcoDI GTCTC 1 cut(s) 885
BfaI CTAG 2 cut(s) 977, 1031
BfmI CTRYAG 2 cut(s) 129, 937
BisI GCNGC 5 cut(s) 188, 261, 549, 892, 940
BlsI GCNGC 5 cut(s) 189, 262, 550, 893, 941
BmcAI AGTACT 1 cut(s) 1078
Bme1390I CCNGG 3 cut(s) 363, 950, 1068
Bme18I GGWCC 1 cut(s) 659
BmgT120I GGNCC 2 cut(s) 659, 783
BmiI GGNNCC 2 cut(s) 418, 569
BmrFI CCNGG 3 cut(s) 363, 950, 1068
BmsI GCATC 3 cut(s) 91, 456, 951
BpmI CTGGAG 1 cut(s) 792
BpuEI CTTGAG 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 1068
BsaAI YACGTR 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 691
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 436, 569
Bse3DI GCAATG 3 cut(s) 288, 783, 1031
BseBI CCWGG 2 cut(s) 363, 950
BseDI CCNNGG 1 cut(s) 691
BseGI GGATG 3 cut(s) 96, 471, 976
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMI GCAATG 3 cut(s) 288, 783, 1031
BseMII CTCAG 2 cut(s) 852, 938
BseNI ACTGG 2 cut(s) 436, 569
BseRI GAGGAG 2 cut(s) 24, 414
BseXI GCAGC 4 cut(s) 174, 272, 903, 951
Bsh1236I CGCG 1 cut(s) 513
BshFI GGCC 3 cut(s) 496, 637, 784
BsiHKAI GWGCWC 2 cut(s) 59, 242
BsiSI CCGG 2 cut(s) 215, 1068
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 885
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 3 cut(s) 496, 637, 784
Bsp1286I GDGCHC 2 cut(s) 59, 242
Bsp143I GATC 7 cut(s) 194, 219, 247, 416, 485, 589, 829
BspACI CCGC 4 cut(s) 251, 420, 548, 993
BspANI GGCC 3 cut(s) 496, 637, 784
BspCNI CTCAG 2 cut(s) 853, 937
BspFNI CGCG 1 cut(s) 513
BspLI GGNNCC 2 cut(s) 418, 569
BspMAI CTGCAG 2 cut(s) 133, 941
BspPI GGATC 5 cut(s) 202, 214, 242, 411, 424
BspQI GCTCTTC 1 cut(s) 337
BsrBI CCGCTC 1 cut(s) 422
BsrDI GCAATG 3 cut(s) 288, 783, 1031
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 2 cut(s) 436, 569
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 691
BssMI GATC 7 cut(s) 194, 219, 247, 416, 485, 589, 829
Bst2UI CCWGG 2 cut(s) 363, 950
Bst4CI ACNGT 1 cut(s) 80
Bst6I CTCTTC 5 cut(s) 171, 337, 432, 960, 1089
BstAPI GCANNNNNTGC 1 cut(s) 945
BstBAI YACGTR 1 cut(s) 505
BstC8I GCNNGC 2 cut(s) 9, 688
BstDEI CTNAG 2 cut(s) 861, 924
BstDSI CCRYGG 1 cut(s) 691
BstF5I GGATG 3 cut(s) 96, 471, 976
BstFNI CGCG 1 cut(s) 513
BstHHI GCGC 1 cut(s) 762
BstKTI GATC 7 cut(s) 197, 222, 250, 419, 488, 592, 832
BstMAI GTCTC 1 cut(s) 885
BstMBI GATC 7 cut(s) 194, 219, 247, 416, 485, 589, 829
BstMWI GCNNNNNNNGC 4 cut(s) 266, 548, 945, 999
BstNI CCWGG 2 cut(s) 363, 950
BstNSI RCATGY 4 cut(s) 11, 51, 631, 802
BstSCI CCNGG 3 cut(s) 361, 948, 1066
BstSFI CTRYAG 2 cut(s) 129, 937
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 4 cut(s) 174, 272, 903, 951
BstX2I RGATCY 1 cut(s) 416
BstXI CCANNNNNNTGG 1 cut(s) 51
BstYI RGATCY 1 cut(s) 416
BsuRI GGCC 3 cut(s) 496, 637, 784
BtgI CCRYGG 1 cut(s) 691
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 3 cut(s) 96, 471, 976
Cac8I GCNNGC 2 cut(s) 9, 688
CaiI CAGNNNCTG 1 cut(s) 945
CfoI GCGC 1 cut(s) 762
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 659, 783
Csp6I GTAC 1 cut(s) 1077
CviAII CATG 6 cut(s) 8, 48, 245, 610, 628, 799
CviQI GTAC 1 cut(s) 1077
DdeI CTNAG 2 cut(s) 861, 924
DpnI GATC 7 cut(s) 196, 221, 249, 418, 487, 591, 831
DpnII GATC 7 cut(s) 194, 219, 247, 416, 485, 589, 829
Eam1104I CTCTTC 5 cut(s) 171, 337, 432, 960, 1089
EarI CTCTTC 5 cut(s) 171, 337, 432, 960, 1089
Ecl136II GAGCTC 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 659
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 2 cut(s) 688, 738
EcoICRI GAGCTC 1 cut(s) 240
EcoRII CCWGG 2 cut(s) 361, 948
EcoT22I ATGCAT 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 242
FaeI CATG 6 cut(s) 11, 51, 248, 613, 631, 802
FatI CATG 6 cut(s) 7, 47, 244, 609, 627, 798
FauNDI CATATG 1 cut(s) 805
FblI GTMKAC 1 cut(s) 617
Fnu4HI GCNGC 5 cut(s) 188, 261, 549, 892, 940
FokI GGATG 3 cut(s) 103, 478, 983
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 5 cut(s) 188, 261, 549, 892, 940
FspBI CTAG 2 cut(s) 977, 1031
FspI TGCGCA 1 cut(s) 761
GlaI GCGC 1 cut(s) 761
GluI GCNGC 5 cut(s) 188, 261, 549, 892, 940
GsuI CTGGAG 1 cut(s) 792
HaeIII GGCC 3 cut(s) 496, 637, 784
HapII CCGG 2 cut(s) 215, 1068
HhaI GCGC 1 cut(s) 762
Hin1II CATG 6 cut(s) 11, 51, 248, 613, 631, 802
Hin6I GCGC 1 cut(s) 760
HinP1I GCGC 1 cut(s) 760
HincII GTYRAC 1 cut(s) 618
HindII GTYRAC 1 cut(s) 618
HindIII AAGCTT 1 cut(s) 204
HinfI GANTC 5 cut(s) 125, 409, 518, 614, 619
HpaII CCGG 2 cut(s) 215, 1068
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 5 cut(s) 110, 368, 618, 700, 909
Hpy188I TCNGA 4 cut(s) 556, 663, 862, 927
Hpy188III TCNNGA 3 cut(s) 38, 748, 771
Hpy8I GTNNAC 5 cut(s) 110, 368, 618, 700, 909
HpyAV CCTTC 1 cut(s) 650
HpyCH4III ACNGT 1 cut(s) 80
HpyCH4IV ACGT 2 cut(s) 454, 504
HpyF10VI GCNNNNNNNGC 4 cut(s) 266, 548, 945, 999
HpyF3I CTNAG 2 cut(s) 861, 924
HpySE526I ACGT 2 cut(s) 454, 504
Hsp92II CATG 6 cut(s) 11, 51, 248, 613, 631, 802
HspAI GCGC 1 cut(s) 760
Kzo9I GATC 7 cut(s) 194, 219, 247, 416, 485, 589, 829
LguI GCTCTTC 1 cut(s) 337
LmnI GCTCC 4 cut(s) 237, 273, 427, 773
Lsp1109I GCAGC 4 cut(s) 174, 272, 903, 951
LweI GCATC 3 cut(s) 91, 456, 951
MaeI CTAG 2 cut(s) 977, 1031
MaeII ACGT 2 cut(s) 454, 504
MaeIII GTNAC 2 cut(s) 74, 679
MalI GATC 7 cut(s) 196, 221, 249, 418, 487, 591, 831
MbiI CCGCTC 1 cut(s) 422
MboI GATC 7 cut(s) 194, 219, 247, 416, 485, 589, 829
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 59, 242
MluCI AATT 8 cut(s) 118, 225, 336, 355, 385, 475, 585, 812
MlyI GAGTC 2 cut(s) 613, 623
MmeI TCCRAC 2 cut(s) 686, 754
Mph1103I ATGCAT 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 932
MseI TTAA 4 cut(s) 147, 209, 330, 1020
MslI CAYNNNNRTG 1 cut(s) 52
MspI CCGG 2 cut(s) 215, 1068
MspR9I CCNGG 3 cut(s) 363, 950, 1068
Mva1269I GAATGC 1 cut(s) 67
MvaI CCWGG 2 cut(s) 363, 950
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 266, 548, 945, 999
NciI CCSGG 1 cut(s) 1068
NdeI CATATG 1 cut(s) 805
NdeII GATC 7 cut(s) 194, 219, 247, 416, 485, 589, 829
NlaIII CATG 6 cut(s) 11, 51, 248, 613, 631, 802
NlaIV GGNNCC 2 cut(s) 418, 569
NmuCI GTSAC 1 cut(s) 74
NsbI TGCGCA 1 cut(s) 761
NsiI ATGCAT 1 cut(s) 9
NspI RCATGY 4 cut(s) 11, 51, 631, 802
PaeI GCATGC 1 cut(s) 11
PciI ACATGT 2 cut(s) 47, 798
PciSI GCTCTTC 1 cut(s) 337
PcsI WCGNNNNNNNCGW 1 cut(s) 510
PctI GAATGC 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 932
PfeI GAWTC 3 cut(s) 125, 409, 518
PkrI GCNGC 5 cut(s) 189, 262, 550, 893, 941
PleI GAGTC 2 cut(s) 613, 622
PpsI GAGTC 2 cut(s) 613, 622
Ppu21I YACGTR 1 cut(s) 505
PscI ACATGT 2 cut(s) 47, 798
PshBI ATTAAT 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 242
Psp6I CCWGG 2 cut(s) 361, 948
PspGI CCWGG 2 cut(s) 361, 948
PspN4I GGNNCC 2 cut(s) 418, 569
PspPI GGNCC 2 cut(s) 659, 783
PstI CTGCAG 2 cut(s) 133, 941
PstNI CAGNNNCTG 1 cut(s) 945
PsuI RGATCY 1 cut(s) 416
RsaI GTAC 1 cut(s) 1078
RsaNI GTAC 1 cut(s) 1077
RseI CAYNNNNRTG 1 cut(s) 52
SacI GAGCTC 1 cut(s) 242
SalI GTCGAC 1 cut(s) 616
SapI GCTCTTC 1 cut(s) 337
SaqAI TTAA 4 cut(s) 147, 209, 330, 1020
SatI GCNGC 5 cut(s) 188, 261, 549, 892, 940
Sau3AI GATC 7 cut(s) 194, 219, 247, 416, 485, 589, 829
Sau96I GGNCC 2 cut(s) 659, 783
ScaI AGTACT 1 cut(s) 1078
SchI GAGTC 2 cut(s) 613, 623
ScrFI CCNGG 3 cut(s) 363, 950, 1068
SduI GDGCHC 2 cut(s) 59, 242
SfaNI GCATC 3 cut(s) 91, 456, 951
SfcI CTRYAG 2 cut(s) 129, 937
SinI GGWCC 1 cut(s) 659
SmiMI CAYNNNNRTG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
SphI GCATGC 1 cut(s) 11
Sse9I AATT 8 cut(s) 118, 225, 336, 355, 385, 475, 585, 812
SsiI CCGC 4 cut(s) 251, 420, 548, 993
SspI AATATT 1 cut(s) 145
SspMI CTAG 2 cut(s) 977, 1031
SstI GAGCTC 1 cut(s) 242
StyD4I CCNGG 3 cut(s) 361, 948, 1066
TaaI ACNGT 1 cut(s) 80
TaiI ACGT 2 cut(s) 457, 507
TaqI TCGA 4 cut(s) 59, 484, 617, 749
TasI AATT 8 cut(s) 118, 225, 336, 355, 385, 475, 585, 812
TatI WGTACW 1 cut(s) 1076
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 3 cut(s) 125, 409, 518
Tru1I TTAA 4 cut(s) 147, 209, 330, 1020
Tru9I TTAA 4 cut(s) 147, 209, 330, 1020
TseFI GTSAC 1 cut(s) 74
TseI GCWGC 4 cut(s) 187, 260, 891, 939
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 3 cut(s) 591, 657, 725
TspGWI ACGGA 2 cut(s) 708, 1101
VpaK11BI GGWCC 1 cut(s) 659
VspI ATTAAT 1 cut(s) 147
XceI RCATGY 4 cut(s) 11, 51, 631, 802
XmiI GTMKAC 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 932
XspI CTAG 2 cut(s) 977, 1031
ZrmI AGTACT 1 cut(s) 1078
Zsp2I ATGCAT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.