Rroxscaffold_2G00154130

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004831
Physical Location & Seq
Reverse (-)
90834161 .. 90836041
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154130.1

Sequence Viewer

Length: 1191 bp
ATGCGTGGGACTTGTTCCAATGTCGATGATTCCCCTCAAGAACAACCATCTGAGCAGCCAATAATATGTGAGTGTTCCAATGAGGAAAATTGTGAATGTAGTAGAGGGATGACTTCTGCTGCACATTTTATAAAACACCTTCCGGGTGAAAGTCAAGGGCCTCAGCTGAATAATGCCGGTGTTGGATTGCTTGCCAATTTTGAATCCGAGAGGGTCAATGGCCATCTTTTATCTCATCCTGCATCTTCATCTTCATCCTCATCCCAAAGATCACATTCACATCAAGCTTTAAAACGTACAAGCAGCCAGGTCTCAAACAAGGCAGAGTGTACTTCATCAATGGTCAATCATAACTCGTCGCAAAGACTAATGGTCCGAAGGAACTCGCATCCTAATTCAGAAGCCACAGTAAGAGGCCTAACAAGTTGTATTCCCAACAGTACTCCAAACCTTATTAACTCTGGAAATTGCACCACATTGCTTTTCGGATCCACTCCTCTTTGCCGATTCTCTCTTTCTCAAGGTCTTTTGGGGGTGCCTTCGAATCTTTTCCATTCTGTAATGCCTGCCATTCAGCTTCCCCACGCAAGTGGAGATTCAACATCTCAAATAGAAGAAGCTCCGGCTACTTTTGATGATAGTGGATTCCCTAGTGAGAACCAAATAATTGATATATGGAGGCAACTCCTCATGGAATCAACTCTATATATACATAGGCCGGCTGATACCTTGTTAGAAGGTTCAAGTTCCGTCTTTAGGGTAACGGCTGACAATGGATCAGTGGACTTCAGACTTGTAACAGAGAACGCTGAAGTTGTTAACCAATCACTAGTGTACTTTTCTATTGAAATGCGCAATCGCTTTGGAGGCATGAGGCTTGATGTGGATAACATGTCATATGAGGAACTATTGGCTTTAGAAGATCATATAGGGTATGTCAGCACAGGATTATCGGAGGAAGCAGCTGTTGCATCTCTCAAACGCAGTAATTATTTTCTTCTTGCTGATGAAAATGCTCAGAAAGAGTTTTGCAGCGTCTGCCAGGAGGATTTTATAGAGGAAGATGAGCTAGGAACATTGGATTGCGGCCACAGCTTCCACATTTGTTGCATCAAACAATGGCTAGGATATAAAAACGTGTGTCCTATATGCAAATCTCCTGCCTTATCTAATACGTTCTCACTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

396

Amino Acids

43.34

Weight (kDa)

5.05

Isoelectric Point (pI)

58.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 341 - 385 7.8e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 344 - 385 1.2e-11 Ring finger domain
zf-C3HC4_2 PF13923 344 - 384 1.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 344 - 384 2.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
Acc16I TGCGCA 1 cut(s) 854
AccB1I GGYRCC 1 cut(s) 535
AciI CCGC 1 cut(s) 1086
AclWI GGATC 3 cut(s) 483, 496, 784
AcoI YGGCCR 2 cut(s) 220, 1087
AcuI CTGAAG 2 cut(s) 772, 831
AfaI GTAC 4 cut(s) 298, 331, 442, 836
AfiI CCNNNNNNNGG 1 cut(s) 756
AflIII ACRYGT 2 cut(s) 891, 1137
AgsI TTSAA 4 cut(s) 203, 600, 744, 848
AhdI GACNNNNNGTC 1 cut(s) 371
AhlI ACTAGT 1 cut(s) 829
AjnI CCWGG 2 cut(s) 306, 1041
AleI CACNNNNGTG 1 cut(s) 588
AluBI AGCT 7 cut(s) 166, 287, 577, 620, 965, 1069, 1095
AluI AGCT 7 cut(s) 166, 287, 577, 620, 965, 1069, 1095
Alw26I GTCTC 1 cut(s) 316
AlwI GGATC 3 cut(s) 483, 496, 784
AlwNI CAGNNNCTG 1 cut(s) 1038
AoxI GGCC 5 cut(s) 158, 220, 415, 716, 1087
ApeKI GCWGC 5 cut(s) 55, 119, 303, 962, 1032
Asp700I GAANNNNTTC 1 cut(s) 548
AspLEI GCGC 1 cut(s) 855
AspS9I GGNCC 2 cut(s) 158, 373
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 158
AsuII TTCGAA 1 cut(s) 542
AvaII GGWCC 1 cut(s) 373
BalI TGGCCA 1 cut(s) 222
BamHI GGATCC 1 cut(s) 488
BanI GGYRCC 1 cut(s) 535
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 5 cut(s) 67, 106, 315, 974, 1044
BccI CCATC 2 cut(s) 55, 231
BceAI ACGGC 1 cut(s) 780
BciT130I CCWGG 2 cut(s) 308, 1043
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 316
BcuI ACTAGT 1 cut(s) 829
BfaI CTAG 4 cut(s) 651, 830, 1070, 1124
BisI GCNGC 6 cut(s) 56, 120, 304, 963, 1033, 1087
BlsI GCNGC 6 cut(s) 57, 121, 305, 964, 1034, 1088
BmcAI AGTACT 1 cut(s) 442
Bme1390I CCNGG 3 cut(s) 144, 308, 1043
Bme18I GGWCC 1 cut(s) 373
BmeRI GACNNNNNGTC 1 cut(s) 371
BmgT120I GGNCC 2 cut(s) 158, 373
BmiI GGNNCC 2 cut(s) 490, 537
BmrFI CCNGG 3 cut(s) 144, 308, 1043
BmsI GCATC 4 cut(s) 251, 397, 980, 1119
Bpu10I CCTNAGC 1 cut(s) 162
Bpu14I TTCGAA 1 cut(s) 542
BpuEI CTTGAG 2 cut(s) 21, 504
BpuMI CCSGG 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 316
Bsc4I CCNNNNNNNGG 1 cut(s) 756
Bse118I RCCGGY 2 cut(s) 176, 718
Bse3DI GCAATG 1 cut(s) 476
BseBI CCWGG 2 cut(s) 308, 1043
BseGI GGATG 5 cut(s) 114, 235, 254, 260, 388
BseLI CCNNNNNNNGG 1 cut(s) 756
BseMI GCAATG 1 cut(s) 476
BseMII CTCAG 3 cut(s) 42, 176, 1031
BseRI GAGGAG 2 cut(s) 486, 677
BseXI GCAGC 5 cut(s) 67, 106, 315, 974, 1044
BsgI GTGCAG 1 cut(s) 105
BshFI GGCC 5 cut(s) 160, 222, 417, 718, 1089
BshNI GGYRCC 1 cut(s) 535
BsiSI CCGG 4 cut(s) 143, 177, 623, 719
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 756
BsmAI GTCTC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 5 cut(s) 160, 222, 417, 718, 1089
Bso31I GGTCTC 1 cut(s) 316
Bsp119I TTCGAA 1 cut(s) 542
Bsp143I GATC 4 cut(s) 269, 488, 776, 922
BspACI CCGC 1 cut(s) 1086
BspANI GGCC 5 cut(s) 160, 222, 417, 718, 1089
BspCNI CTCAG 3 cut(s) 43, 175, 1030
BspLI GGNNCC 2 cut(s) 490, 537
BspPI GGATC 3 cut(s) 483, 496, 784
BspT104I TTCGAA 1 cut(s) 542
BspT107I GGYRCC 1 cut(s) 535
BspTNI GGTCTC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 476
BsrFI RCCGGY 2 cut(s) 176, 718
BssAI RCCGGY 2 cut(s) 176, 718
BssMI GATC 4 cut(s) 269, 488, 776, 922
Bst2UI CCWGG 2 cut(s) 308, 1043
Bst4CI ACNGT 2 cut(s) 409, 440
BstAPI GCANNNNNTGC 2 cut(s) 968, 1038
BstBI TTCGAA 1 cut(s) 542
BstC8I GCNNGC 3 cut(s) 192, 567, 720
BstDEI CTNAG 3 cut(s) 51, 162, 1017
BstF5I GGATG 5 cut(s) 114, 235, 254, 260, 388
BstHHI GCGC 1 cut(s) 855
BstKTI GATC 4 cut(s) 272, 491, 779, 925
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 4 cut(s) 269, 488, 776, 922
BstMWI GCNNNNNNNGC 4 cut(s) 867, 968, 1038, 1092
BstNI CCWGG 2 cut(s) 308, 1043
BstNSI RCATGY 1 cut(s) 895
BstSCI CCNGG 3 cut(s) 142, 306, 1041
BstV1I GCAGC 5 cut(s) 67, 106, 315, 974, 1044
BstX2I RGATCY 1 cut(s) 488
BstXI CCANNNNNNTGG 1 cut(s) 590
BstYI RGATCY 1 cut(s) 488
BsuRI GGCC 5 cut(s) 160, 222, 417, 718, 1089
BtsCI GGATG 5 cut(s) 114, 235, 254, 260, 388
BtsIMutI CAGTG 1 cut(s) 786
Cac8I GCNNGC 3 cut(s) 192, 567, 720
CaiI CAGNNNCTG 1 cut(s) 1038
CfoI GCGC 1 cut(s) 855
Cfr10I RCCGGY 2 cut(s) 176, 718
Cfr13I GGNCC 2 cut(s) 158, 373
CseI GACGC 1 cut(s) 1024
Csp6I GTAC 4 cut(s) 297, 330, 441, 835
CspCI CAANNNNNGTGG 2 cut(s) 1088, 1123
CviAII CATG 3 cut(s) 691, 871, 892
CviQI GTAC 4 cut(s) 297, 330, 441, 835
DdeI CTNAG 3 cut(s) 51, 162, 1017
DpnI GATC 4 cut(s) 271, 490, 778, 924
DpnII GATC 4 cut(s) 269, 488, 776, 922
DraI TTTAAA 1 cut(s) 291
DriI GACNNNNNGTC 1 cut(s) 371
EaeI YGGCCR 2 cut(s) 220, 1087
Eam1105I GACNNNNNGTC 1 cut(s) 371
Eco147I AGGCCT 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 2 cut(s) 772, 831
EcoO109I RGGNCCY 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 306, 1041
FaeI CATG 3 cut(s) 694, 874, 895
FaqI GGGAC 1 cut(s) 22
FatI CATG 3 cut(s) 690, 870, 891
FauNDI CATATG 1 cut(s) 898
Fnu4HI GCNGC 6 cut(s) 56, 120, 304, 963, 1033, 1087
FokI GGATG 5 cut(s) 121, 222, 241, 247, 375
Fsp4HI GCNGC 6 cut(s) 56, 120, 304, 963, 1033, 1087
FspBI CTAG 4 cut(s) 651, 830, 1070, 1124
FspI TGCGCA 1 cut(s) 854
GlaI GCGC 1 cut(s) 854
GluI GCNGC 6 cut(s) 56, 120, 304, 963, 1033, 1087
HaeIII GGCC 5 cut(s) 160, 222, 417, 718, 1089
HapII CCGG 4 cut(s) 143, 177, 623, 719
HgaI GACGC 1 cut(s) 1024
HhaI GCGC 1 cut(s) 855
Hin1II CATG 3 cut(s) 694, 874, 895
Hin6I GCGC 1 cut(s) 853
HinP1I GCGC 1 cut(s) 853
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 7 cut(s) 29, 203, 507, 544, 596, 645, 695
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 4 cut(s) 143, 177, 623, 719
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 4 cut(s) 330, 784, 820, 835
Hpy188I TCNGA 8 cut(s) 52, 208, 377, 400, 488, 791, 955, 1020
Hpy188III TCNNGA 2 cut(s) 38, 462
Hpy8I GTNNAC 4 cut(s) 330, 784, 820, 835
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 4 cut(s) 149, 372, 549, 731
HpyCH4III ACNGT 2 cut(s) 409, 440
HpyCH4IV ACGT 3 cut(s) 295, 1137, 1175
HpyCH4V TGCA 7 cut(s) 122, 242, 471, 971, 1032, 1110, 1152
HpyF10VI GCNNNNNNNGC 4 cut(s) 867, 968, 1038, 1092
HpyF3I CTNAG 3 cut(s) 51, 162, 1017
HpySE526I ACGT 3 cut(s) 295, 1137, 1175
Hsp92II CATG 3 cut(s) 694, 874, 895
HspAI GCGC 1 cut(s) 853
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
KspAI GTTAAC 1 cut(s) 820
Kzo9I GATC 4 cut(s) 269, 488, 776, 922
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 5 cut(s) 67, 106, 315, 974, 1044
LweI GCATC 4 cut(s) 251, 397, 980, 1119
MaeI CTAG 4 cut(s) 651, 830, 1070, 1124
MaeII ACGT 3 cut(s) 295, 1137, 1175
MaeIII GTNAC 2 cut(s) 760, 796
MalI GATC 4 cut(s) 271, 490, 778, 924
MboI GATC 4 cut(s) 269, 488, 776, 922
MboII GAAGA 6 cut(s) 237, 243, 626, 932, 989, 1073
MflI RGATCY 1 cut(s) 488
MlsI TGGCCA 1 cut(s) 222
MluCI AATT 6 cut(s) 88, 196, 394, 466, 666, 988
MluNI TGGCCA 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 163
Mox20I TGGCCA 1 cut(s) 222
MroNI GCCGGC 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 548
MscI TGGCCA 1 cut(s) 222
MseI TTAA 4 cut(s) 290, 456, 819, 1189
MslI CAYNNNNRTG 1 cut(s) 588
Msp20I TGGCCA 1 cut(s) 222
MspA1I CMGCKG 2 cut(s) 166, 965
MspI CCGG 4 cut(s) 143, 177, 623, 719
MspR9I CCNGG 3 cut(s) 144, 308, 1043
MvaI CCWGG 2 cut(s) 308, 1043
MwoI GCNNNNNNNGC 4 cut(s) 867, 968, 1038, 1092
NaeI GCCGGC 1 cut(s) 720
NciI CCSGG 1 cut(s) 144
NdeI CATATG 1 cut(s) 898
NdeII GATC 4 cut(s) 269, 488, 776, 922
NgoMIV GCCGGC 1 cut(s) 718
NlaIII CATG 3 cut(s) 694, 874, 895
NlaIV GGNNCC 2 cut(s) 490, 537
NsbI TGCGCA 1 cut(s) 854
NspI RCATGY 1 cut(s) 895
NspV TTCGAA 1 cut(s) 542
OliI CACNNNNGTG 1 cut(s) 588
PceI AGGCCT 1 cut(s) 417
PciI ACATGT 1 cut(s) 891
PdiI GCCGGC 1 cut(s) 720
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 7 cut(s) 29, 203, 507, 544, 596, 645, 695
PkrI GCNGC 6 cut(s) 57, 121, 305, 964, 1034, 1088
PscI ACATGT 1 cut(s) 891
PsiI TTATAA 1 cut(s) 131
Psp6I CCWGG 2 cut(s) 306, 1041
PspGI CCWGG 2 cut(s) 306, 1041
PspN4I GGNNCC 2 cut(s) 490, 537
PspPI GGNCC 2 cut(s) 158, 373
PstNI CAGNNNCTG 1 cut(s) 1038
PsuI RGATCY 1 cut(s) 488
PvuII CAGCTG 2 cut(s) 166, 965
RsaI GTAC 4 cut(s) 298, 331, 442, 836
RsaNI GTAC 4 cut(s) 297, 330, 441, 835
RseI CAYNNNNRTG 1 cut(s) 588
SaqAI TTAA 4 cut(s) 290, 456, 819, 1189
SatI GCNGC 6 cut(s) 56, 120, 304, 963, 1033, 1087
Sau3AI GATC 4 cut(s) 269, 488, 776, 922
Sau96I GGNCC 2 cut(s) 158, 373
ScaI AGTACT 1 cut(s) 442
ScrFI CCNGG 3 cut(s) 144, 308, 1043
SfaNI GCATC 4 cut(s) 251, 397, 980, 1119
SfuI TTCGAA 1 cut(s) 542
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 2 cut(s) 36, 519
SmoI CTYRAG 2 cut(s) 36, 519
SpeI ACTAGT 1 cut(s) 829
Sse9I AATT 6 cut(s) 88, 196, 394, 466, 666, 988
SseBI AGGCCT 1 cut(s) 417
SsiI CCGC 1 cut(s) 1086
SspMI CTAG 4 cut(s) 651, 830, 1070, 1124
StuI AGGCCT 1 cut(s) 417
StyD4I CCNGG 3 cut(s) 142, 306, 1041
TaaI ACNGT 2 cut(s) 409, 440
TaiI ACGT 3 cut(s) 298, 1140, 1178
TaqI TCGA 2 cut(s) 24, 542
TasI AATT 6 cut(s) 88, 196, 394, 466, 666, 988
TatI WGTACW 3 cut(s) 329, 440, 834
TauI GCSGC 1 cut(s) 1089
TfiI GAWTC 7 cut(s) 29, 203, 507, 544, 596, 645, 695
Tru1I TTAA 4 cut(s) 290, 456, 819, 1189
Tru9I TTAA 4 cut(s) 290, 456, 819, 1189
TscAI CASTG 1 cut(s) 786
TseI GCWGC 5 cut(s) 55, 119, 303, 962, 1032
TspDTI ATGAA 4 cut(s) 237, 243, 324, 1023
TspGWI ACGGA 1 cut(s) 739
TspRI CASTG 1 cut(s) 786
VpaK11BI GGWCC 1 cut(s) 373
XceI RCATGY 1 cut(s) 895
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 4 cut(s) 651, 830, 1070, 1124
ZrmI AGTACT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.